JP2017096985A5 - - Google Patents

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JP2017096985A5
JP2017096985A5 JP2017034071A JP2017034071A JP2017096985A5 JP 2017096985 A5 JP2017096985 A5 JP 2017096985A5 JP 2017034071 A JP2017034071 A JP 2017034071A JP 2017034071 A JP2017034071 A JP 2017034071A JP 2017096985 A5 JP2017096985 A5 JP 2017096985A5
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sample
labeling substance
varicella
zoster virus
substance holding
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JP2017034071A
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Japanese (ja)
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JP2017096985A (en
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Priority to JP2017034071A priority Critical patent/JP2017096985A/en
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試料添加部、標識物質保持部、検出部を有するクロマトグラフ媒体部及び吸収部を含む、水痘帯状疱疹ウイルス(Varicella−Zoster Virus、VZV)を検出するための免疫クロマト分析装置であって、
前記標識物質保持部及び検出部の両方が、配列番号1のアミノ酸配列からなる前記水痘帯状疱疹ウイルスの糖タンパク質E(VZVgE)のアミノ酸配列48−88番目を認識する抗体を含有する、免疫クロマト分析装置。
An immunochromatography analyzer for detecting varicella-zoster virus (VZV), comprising a sample addition part, a labeling substance holding part, a chromatographic medium part having a detection part and an absorption part,
Immunochromatographic analysis in which both the labeling substance holding part and the detection part contain an antibody recognizing the amino acid sequence 48-88 of glycoprotein E (VZVgE) of the varicella-zoster virus consisting of the amino acid sequence of SEQ ID NO: 1. apparatus.
前記試料添加部に添加する試料が、前記水痘帯状疱疹ウイルスを含む水疱内容液である、請求項に記載の免疫クロマト分析装置。 The immunochromatographic analyzer according to claim 1 , wherein the sample added to the sample addition part is a blister content liquid containing the varicella-zoster virus. 前記標識物質保持部が含有する標識物質が金コロイド粒子であり、前記標識物質保持部が前記金コロイド粒子を、0.05〜1.5μg/cm含有する、請求項1または2に記載の免疫クロマト分析装置。 The labeling substance labeling substance holding portion contains a colloidal gold particle, the labeling substance holding section the gold colloid particles, 0.05~1.5μg / cm 2 containing, according to claim 1 or 2 Immunochromatographic analyzer. 請求項1〜のいずれか1項に記載の免疫クロマト分析装置と、検体を希釈して展開するための検体希釈液とを含む、免疫クロマト分析キット。 An immunochromatography analysis kit comprising the immunochromatography analyzer according to any one of claims 1 to 3 and a sample diluent for diluting and developing the sample. 前記検体希釈液が少なくとも1種の非イオン性界面活性剤を含有する、請求項に記載の免疫クロマト分析キット。 The immunochromatographic analysis kit according to claim 4 , wherein the specimen diluent contains at least one nonionic surfactant. 試料添加部、標識物質保持部、検出部を有するクロマトグラフ媒体部及び吸収部を含む免疫クロマト分析装置を用いて、検体中の水痘帯状疱疹ウイルス(Varicella−Zoster Virus、VZV)を検出する方法であり、前記免疫クロマト分析装置の前記標識物質保持部及び検出部の両方が、配列番号1のアミノ酸配列からなる前記水痘帯状疱疹ウイルスの糖タンパク質E(VZVgE)のアミノ酸配列48−88番目を認識する抗体を含有し、以下の工程(1)〜(4)を含む免疫クロマト分析方法。
(1)検体希釈液により検体を希釈した検体含有液を試料添加部に添加する工程
(2)標識物質保持部に保持されている抗体により水痘帯状疱疹ウイルスを認識させる工程
(3)前記検体及び抗体を移動相としてクロマトグラフ媒体部に展開させる工程
(4)展開された移動相中の水痘帯状疱疹ウイルスを検出部に含まれる抗体により検出する工程
A method for detecting varicella-zoster virus (VZV) in a specimen using an immunochromatography analyzer including a sample addition part, a labeling substance holding part, a chromatographic medium part having a detection part, and an absorption part. Yes, both the labeling substance holding part and the detection part of the immunochromatography analyzer recognize the amino acid sequence 48-88 of the varicella-zoster virus glycoprotein E (VZVgE) consisting of the amino acid sequence of SEQ ID NO: 1. An immunochromatographic analysis method comprising an antibody and comprising the following steps (1) to (4).
(1) A step of adding a sample-containing solution obtained by diluting a sample with a sample dilution solution to a sample addition unit (2) a step of recognizing varicella-zoster virus by an antibody held in a labeling substance holding unit (3) the sample and (4) A step of detecting varicella-zoster virus in the developed mobile phase with an antibody contained in the detection unit.
JP2017034071A 2017-02-24 2017-02-24 Immuno chromatographic analysis device for varicella-zoster virus detection Pending JP2017096985A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2017034071A JP2017096985A (en) 2017-02-24 2017-02-24 Immuno chromatographic analysis device for varicella-zoster virus detection

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Application Number Priority Date Filing Date Title
JP2017034071A JP2017096985A (en) 2017-02-24 2017-02-24 Immuno chromatographic analysis device for varicella-zoster virus detection

Related Parent Applications (1)

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JP2015230928A Division JP6367783B2 (en) 2015-11-26 2015-11-26 Immunochromatographic analyzer for detection of varicella-zoster virus

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JP2017096985A JP2017096985A (en) 2017-06-01
JP2017096985A5 true JP2017096985A5 (en) 2019-08-29

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117074673B (en) * 2023-10-18 2024-05-28 北京华诺泰生物医药科技有限公司 Method for quantitatively detecting specific activity of recombinant herpes zoster vaccine or/and stock solution

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GB9413751D0 (en) * 1994-07-07 1994-08-24 British Bio Technology Immunodominant peptides
JP4559510B2 (en) * 2008-07-14 2010-10-06 田中貴金属工業株式会社 Developing solution for immunochromatography and measurement method using the same

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