JP2017070446A - Living body component measurement device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a living body component measurement device capable of measuring a specific component contained in body fluid in real time (immediately).SOLUTION: A living body component measurement device 10 includes a sampling part 1 indwelt in a living body for sampling body fluid, a stimulation responsive gel 2 that expands or contracts by the stimulation by a specific component contained in the body fluid, and a storage part 3 connected to the sampling part 1 for storing the stimulation responsive gel 2. The sampling part 1 includes a needle body 11 having a through-hole 111, and the through-hole 111 is preferably communicated with the storage part 3. The stimulation responsive gel 2 is preferably the one that responds to the stimulation by lactic acid as the specific component.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a biological component measuring apparatus.

  At present, as a method for obtaining in-vivo biological information, a biochemical test for examining a composition in blood obtained by blood sampling is usually performed. Such a test is generally performed in a medical institution.

  On the other hand, a blood measurement device is known in which a diabetic patient can check the blood glucose level by himself (see, for example, Patent Document 1).

  In such a blood measuring apparatus, since blood is once collected and then analyzed, the components in the blood cannot be analyzed in real time.

Japanese Patent Application Laid-Open No. 11-24201

  An object of the present invention is to provide a biological component measuring apparatus capable of measuring a specific component contained in a body fluid in real time (immediately).

The living body component measuring apparatus of the present invention is indwelled in a living body, and a collecting unit for collecting a body fluid;
A stimulus-responsive gel that expands or contracts by stimulation with a specific component contained in the body fluid;
While containing the stimulus-responsive gel, and a storage unit connected to the collection unit,
It is characterized by providing.

  Thereby, the biological component measuring device which can measure the specific component contained in a bodily fluid in real time (instantly) can be provided.

In the biological component measurement apparatus of the present invention, the collection unit includes a needle-like body having a through hole,
The through hole is preferably in communication with the accommodating portion.
Thereby, collection | collection of a bodily fluid and derivation | leading-out of the bodily fluid to an accommodating part can be performed more efficiently.

  In the living body component measuring apparatus of the present invention, it is preferable that the stimulus-responsive gel is disposed at a position separated from the through hole.

  Thereby, while being able to contact the bodily fluid supplied from the collection | collection part with a stimulus responsive gel efficiently, the dispersion | variation in the quantity of the bodily fluid supplied to each site | part of a stimulus responsive gel can be suppressed, and a stimulus response It is possible to suppress variation in the rate of change in volume at each site of the gel. As a result, the detection speed of the specific component can be improved, and the detection accuracy and reliability can be further improved.

  In the biological component measurement apparatus of the present invention, it is preferable that the collection unit includes a plurality of the needle-like bodies.

  Thereby, collection | collection of a bodily fluid and derivation | leading-out of the bodily fluid to an accommodating part can be performed still more efficiently. In addition, variation in the amount of body fluid supplied to each part of the stimulus-responsive gel can be suppressed, and variation in volume change rate in each part of the stimulus-responsive gel can be suppressed. As a result, the accuracy and reliability of detection of the specific component can be further improved.

  In the biological component measuring apparatus of the present invention, it is preferable that the biological component measuring apparatus further includes a decompression unit that makes the space in the housing portion negative.

  As a result, the pressure in the housing portion can be made lower than the pressure in the living body in which at least a part of the collecting portion is inserted and the pressure in the internal space of the collecting portion inserted in the living body, and more efficiently from the living body. The body fluid can be collected well, and the collected body fluid can be supplied to the storage unit. As a result, it is possible to further improve the detection speed of the specific component.

  In the living body component measuring apparatus of the present invention, it is preferable that the collection unit includes a flexible hollow member.

  Thereby, the length of the collection part inserted (for example, puncture) in a living body can be made longer. In addition, the safety of the biological component measuring device can be made higher.

  The biological component measuring apparatus of the present invention preferably includes a hollow fiber or a porous tube as the hollow member.

  Thereby, a bodily fluid can be collected more efficiently. Moreover, the safety | security with respect to a biological body can be made more excellent.

  The biological component measuring apparatus of the present invention preferably includes a collection assisting liquid supply unit that supplies a collection assisting liquid for assisting the collection of the body fluid into the living body.

  Thereby, the body fluid to be collected can be efficiently guided to the collection unit, and the detection efficiency of the specific component can be further improved.

  In the living body component measuring apparatus of the present invention, it is preferable that the collection auxiliary liquid supply unit is connected to the collection unit.

  Thereby, a body fluid (in particular, a body fluid diluted with a collection auxiliary liquid) can be collected more efficiently.

  In the biological component measuring apparatus of the present invention, it is preferable that the stimulus-responsive gel responds to stimulation by lactic acid as the specific component.

  Thereby, the specific component (lactic acid) can be detected with higher quantitativeness in a wide concentration range from low concentration to high concentration. Moreover, it can respond suitably also to a serious disease with a shock such as heart disease or sepsis.

  In the biological component measurement apparatus of the present invention, it is preferable that the stimulus-responsive gel includes a water-soluble vinyl monomer as a constituent monomer and a polymer having a phenylboronic acid structure.

  Thereby, the rate of change of the volume of the stimulus-responsive gel due to the concentration change of the specific component can be increased, and the specific component can be quantitatively detected more suitably. In particular, detection of lactic acid or the like can be performed more suitably.

  In the biological component measuring apparatus of the present invention, the polymer preferably contains a monomer having a phenylboronic acid structure as a constituent component.

  Thereby, the ratio etc. of the phenylboronic acid structure which a polymer has can be adjusted easily. Further, the rigidity of the polymer can be more easily performed. In addition, the chemical stability of the polymer can be further improved, and unintended deterioration, modification, and the like of the polymer can be more effectively suppressed.

  In the biological component measuring apparatus of the present invention, the polymer preferably contains N-hydroxyethylacrylamide as the water-soluble vinyl monomer.

  Thereby, the rate of change of the volume of the stimulus-responsive gel due to the concentration change of the specific component can be further increased, and the specific component can be quantitatively detected more suitably. In particular, detection of lactic acid or the like can be performed more suitably.

In the biological component measuring apparatus of the present invention, the stimulus-responsive gel includes a first polymer having a phenylboronic acid structure, and a second polymer having an OH group and having a network structure,
It is preferable that a polymer chain of the first polymer enters the network of the second polymer.

  Thereby, the reactivity (sensitivity) with respect to the specific component of a stimulus responsive gel can be made higher. In addition, the entire stimulus-responsive gel can be deformed with high uniformity, the structural color variation is suppressed at each site of the stimulus-responsive gel, and the concentration of the predetermined component can be easily and accurately determined. In addition, the predetermined stimulus can be detected stably over a long period of time, and the durability of the stimulus-responsive gel becomes better.

  In the biological component measuring apparatus of the present invention, it is preferable that the stimulus-responsive gel is formed by dispersing particles having an average particle diameter of 10 nm or more and 1000 nm or less.

  Thereby, the structural color by Bragg reflection can be recognized, and the specific component can be quantified more easily and more accurately by the color tone.

  In the biological component measurement apparatus of the present invention, it is preferable that the stimulus-responsive gel contains a conductive substance.

  Thereby, by obtaining the resistance value (resistivity) of the stimulus-responsive gel, the expansion / contraction state of the stimulus-responsive gel, that is, the concentration of the specific component can be easily and stably determined.

It is typical sectional drawing for demonstrating 1st Embodiment of a biological component measuring apparatus. It is typical sectional drawing for demonstrating 2nd Embodiment of a biological component measuring apparatus. It is a flowchart which shows suitable embodiment of the usage method of a biological component measuring apparatus.

Hereinafter, preferred embodiments will be described in detail with reference to the accompanying drawings.
<Biological component measuring device>

[First Embodiment]
First, the biological component measuring apparatus according to the first embodiment will be described.
FIG. 1 is a schematic cross-sectional view for explaining a first embodiment of a biological component measuring apparatus.

  The biological component measuring apparatus 10 is placed in a living body and collects a body fluid and includes a specific component contained in the body fluid (regardless of whether it is in a dissolved state or a dispersed state). A stimulus-responsive gel 2 that expands or contracts by stimulation and a storage unit 3 that stores the stimulus-responsive gel 2 and is connected to the collection unit 1 are provided. The stimulus responsiveness refers to expansion or contraction due to a specific component.

  As described above, the biological component measuring apparatus 10 is configured so that the body fluid collected from the living body is brought into contact with the stimulus-responsive gel 2 in the apparatus.

  Thereby, the specific component contained in the body fluid can be detected in real time (immediately). In particular, since the stimulus-responsive gel 2 generally changes in volume (expansion rate and contraction rate change) according to the concentration of the specific component, the specific component can be quantitatively detected.

  In addition, since the stimulus-responsive gel 2 reversibly changes its volume (the expansion rate and contraction rate change) according to the concentration of the specific component, the specific component in the body fluid can be monitored over a long period of time. can do. Therefore, conventionally, when examining the body fluid composition at each time point, a treatment (blood collection or the like) for collecting body fluid has to be performed a plurality of times, and the burden on the patient or the like has been great. It is not necessary to perform such processes a plurality of times, and the burden on the patient can be greatly reduced.

  Conventionally, when examining body fluids, generally, in a medical institution, a body fluid is once collected (in the case of blood, blood is collected), and a biochemical test is performed on the collected body fluid. In such a conventional method, it takes a relatively long time from obtaining a bodily fluid to obtaining a test result, and it is difficult to grasp the state of the bodily fluid in real time. Especially for patients with heart disease, sepsis, etc. who are in a shocked state or who may be in a shock state, or patients with organ failure, etc. In order to quickly detect the patient, a bedside patient condition monitoring device is required, but such a requirement is not satisfied. In addition, in the above method, it is often necessary to collect a bodily fluid a plurality of times (multiple times) after a predetermined period of time, and each time a lot of doctors, nurses, laboratory technicians, etc. The labor of the person was necessary.

  On the other hand, the biological component measuring apparatus 10 can perform a process from collection of body fluid to detection of a specific component contained in the body fluid (particularly, quantitative detection of the specific component) in a single apparatus. The body fluid can be inspected in a short time from the indwelling of the body, the state of the body fluid can be grasped in real time, and it is possible to cope with a serious disease patient and the like. In addition, once the collection unit 1 is placed, monitoring of changes in the composition of body fluid over time, etc. can be suitably performed, and the labor of doctors, nurses, laboratory technicians, etc. can be greatly reduced. Can do. In particular, even in places where there are no medical personnel such as doctors and nurses such as the patient's home, monitoring of changes in body fluid composition over time can be suitably performed. In addition, if necessary, by sending the monitoring result data to a medical institution or the like, the visit frequency of the patient to the medical institution or the like can be reduced, and the burden on the patient himself can be reduced. Moreover, since the medical personnel can take more prompt treatment (including preventive treatment) and treatment from the monitor results, it is possible to more effectively suppress exacerbation of the patient's symptoms, etc. Early recovery can be achieved.

  In this specification, the body fluid refers to a fluid contained in a living body, and examples thereof include blood, lymph, interstitial fluid, bone marrow fluid, saliva, gastric fluid, urine, amniotic fluid, and the like.

  In the present embodiment, the collection unit 1 includes a needle-like body 11 having a through hole (hollow part) 111 communicating with the storage unit 3. The through hole 111 functions as a body fluid flow path.

  With such a configuration, collection of body fluid and derivation of body fluid to the storage unit 3 can be performed more efficiently.

  Moreover, in this embodiment, the collection part 1 has the some acicular body 11 arrange | positioned so that the several site | parts from which the stimulus responsive gel 2 differs may be opposed. In other words, the accommodating portion 3 communicates the space where the stimulus-responsive gel 2 is provided with the outside by the plurality of needle-like bodies 11. And the surface in which the through-hole 111 of the some acicular body 11 of the accommodating part 3 is formed, and the surface in which the stimulus responsive gel 2 of the accommodating part 3 is provided are facing.

  Thereby, collection | collection of a bodily fluid and derivation | leading-out of the bodily fluid to the accommodating part 3 can be performed still more efficiently. Moreover, the variation in the amount of body fluid supplied to each part of the stimulus responsive gel 2 can be suppressed, and the variation in the volume change rate at each part of the stimulus responsive gel 2 can be suppressed. As a result, the accuracy and reliability of detection of the specific component can be further improved.

  The constituent material of the acicular body 11 is not specifically limited, For example, various metal materials and various plastic materials can be used. Among these, a metal material (for example, stainless steel (ISO9626 compatible product, etc.), titanium, titanium alloy, etc.) that is hard and has excellent durability and safety to living bodies is preferable.

  As the collection unit 1, a microneedle can be suitably used. The microneedle refers to a micrometer-sized needle, and here, refers to a hollow needle-like body 11 having a through hole in the extending direction of the needle-like body 11.

  The outer diameter of the through hole of the microneedle is preferably 1 μm or more and 300 μm or less. Moreover, when the collection part 1 is a microneedle, it is preferable that the length of the acicular body 11 is 10 micrometers or more and 2500 micrometers or less. If the length of the needle-like body 11 is about 2500 μm, it can be inserted to the vicinity of the boundary between the dermis and the subcutaneous tissue.

  The stimulus-responsive gel 2 is reversibly expanded or contracted by stimulation with a specific component contained in a body fluid.

  The specific component to which the stimulus responsive gel 2 reacts can be adjusted by the composition of the stimulus responsive gel 2 and the like.

The stimulus-responsive gel 2 will be described in detail later.
The storage unit 3 stores the stimulus-responsive gel 2 and is connected to the collection unit 1.

  With such a configuration, the body fluid collected by the collection unit 1 is supplied (derived) to the storage unit 3 and comes into contact with the stimulus-responsive gel 2 stored in the internal space of the storage unit 3. And according to the density | concentration of the specific component contained in the bodily fluid which contacted the stimulus responsive gel 2, the stimulus responsive gel 2 expands or contracts. In addition, the reaction between the stimulus-responsive gel 2 and the specific component is generally sufficiently fast.

  Thus, the specific component contained in the bodily fluid is contacted in real time (immediately) by bringing the collected bodily fluid into contact with the stimulus-responsive gel 2 that functions as a sensor of the specific component without taking it out of the apparatus. Can be detected.

  The stimuli-responsive gel 2 only needs to be accommodated in the accommodating part 3, and the installation site in the accommodating part 3 is not particularly limited. However, in the illustrated configuration, the stimuli-responsive gel 2 is separated from the through hole 111. It is arranged at the position.

  More specifically, in this embodiment, the stimulus-responsive gel 2 is a surface (upper surface in the drawing) that faces the surface (the lower surface in the drawing) in which the through-hole 111 is provided in the housing portion 3. ), And a space is provided between the surface (the lower surface in the figure) where the through-hole 111 of the housing portion 3 is provided and the stimulus-responsive gel 2.

  With such a configuration, the body fluid supplied from the collection unit 1 can be efficiently brought into contact with the stimulus-responsive gel 2, and variation in the amount of body fluid supplied to each part of the stimulus-responsive gel 2 is suppressed. It is possible to suppress variation in the rate of change in volume at each site of the stimulus-responsive gel 2. As a result, the detection speed of the specific component can be improved, and the detection accuracy and reliability can be further improved.

  The container 3 is disposed outside the living body from which the body fluid is collected when the biological component measuring apparatus 10 is used.

  The housing part 3 may be made of any material. Examples of the constituent material of the housing part 3 include various resins such as polyethylene terephthalate, polycarbonate, and acrylonitrile-butadiene-styrene copolymer (ABS resin). Examples include materials, various metal materials, various ceramic materials, and various glasses.

When the stimuli-responsive gel 2 includes particles (fine particles) as described later, it is preferable that the container 3 is made of a transparent material.
Thereby, the color tone of the stimulus-responsive gel 2 can be suitably recognized from the outside.

In particular, the visible light transmittance (for example, the light transmittance at a wavelength of 560 nm) of the constituent material of the housing portion 3 is preferably 70% or more, and more preferably 80% or more.
Thereby, the effects as described above are more remarkably exhibited.

  Moreover, the biological component measuring apparatus 10 of the present embodiment further includes a decompression unit 4 that makes the space in the storage unit 3 negative pressure in addition to the collection unit 1, the stimulus-responsive gel 2 and the storage unit 3 described above. Yes.

  Thereby, the pressure in the accommodating part 3 is lower than the pressure in the living body into which at least a part of the collecting part 1 is inserted and the pressure in the internal space (through hole 111) of the collecting part 1 inserted in the living body. The body fluid can be collected from the living body more efficiently, and the collected body fluid can be supplied to the storage unit 3. As a result, it is possible to further improve the detection speed of the specific component.

  As the decompression means 4, for example, various pumps such as a micro pump can be used.

In addition, the biological component measurement apparatus 10 includes a drainage unit (not shown) that discharges the body fluid supplied to the storage unit 3 to the outside of the storage unit 3.
Thereby, a specific component can be detected stably over a longer period of time.

<Stimulus responsive gel>
Hereinafter, the stimulus-responsive gel 2 constituting the biological component measuring apparatus 10 will be described in detail.
The stimulus-responsive gel 2 is reversibly expanded or contracted by stimulation with a specific component contained in a body fluid.

  The stimulus-responsive gel 2 may be made of any material as long as it responds to stimulation by a specific component, but is usually made of a material containing a polymer material and a solvent. It is a thing. Stimulation refers to various chemical reactions and physical actions.

(Polymer material (polymer))
The polymer material constituting the stimulus-responsive gel 2 is an important component for detecting a specific component, and its structure varies depending on the type of stimulus to be detected (type of the specific component).

  The polymer material constituting the stimulus-responsive gel 2 is not particularly limited, and can be selected depending on the specific component to be detected.

Hereinafter, specific examples of the polymer material constituting the stimulus-responsive gel 2 will be described.
As a polymeric material which comprises the stimulus responsive gel 2, what was obtained by making a monomer (monomer), a polymerization initiator, a crosslinking agent react, etc. can be used, for example.

  Examples of the monomer include acrylamide, N-methylacrylamide, N-isopropylacrylamide, N, N-dimethylacrylamide, N, N-dimethylaminopropylacrylamide, N, N-dimethylaminopropylacrylamide, various quaternary salts, and acryloyl. Morpholine, N, N-dimethylaminoethyl acrylate quaternary salts, acrylic acid, various alkyl acrylates, methacrylic acid, various alkyl methacrylates, 2-hydroxyethyl methacrylate, glycerol monomethacrylate, N-vinylpyrrolidone, acrylonitrile, styrene, polyethylene glycol Diacrylate, 1,6-hexanediol diacrylate, neopentyl glycol diacrylate, tripropylene glycol diacrylate, poly Lopylene glycol diacrylate, 2,2-bis [4- (acryloxydiethoxy) phenyl] propane, 2,2-bis [4- (acryloxypolyethoxy) phenyl] propane, 2-hydroxy-1-acryloxy-3 -Methacryloxypropane, 2,2-bis [4- (acryloxypolypropoxy) phenyl] propane, ethylene glycol dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, 1,3-butylene glycol di Methacrylate, 1,6-hexanediol dimethacrylate, neopentyl glycol dimethacrylate, polypropylene glycol dimethacrylate, 2-hydroxy-1,3-dimethacryloxypropane 2,2-bis [4- (methacryloxyethoxy) phenyl] propane, 2,2-bis [4- (methacryloxyethoxydiethoxy) phenyl] propane, 2,2-bis [4- (methacryloxyethoxypolyethoxy) ) Phenyl] propane, trimethylolpropane trimethacrylate, tetramethylolmethane trimethacrylate, trimethylolpropane triacrylate, tetramethylolmethane triacrylate, tetramethylolmethane tetraacrylate, dipentaerythritol hexaacrylate, N, N′-methylenebisacrylamide, N, N′-methylenebismethacrylamide, diethylene glycol diallyl ether, divinylbenzene and the like can be mentioned.

  In addition, examples of the functional group capable of interacting with saccharides (for example, glucose, fructose, etc.) include boronic acid groups (particularly phenylboronic acid groups), and therefore, using a monomer having a boronic acid group. Also good. Examples of such boronic acid group-containing monomers include acryloylaminobenzeneboronic acid, methacryloylaminobenzeneboronic acid, 4-vinylbenzeneboronic acid, and the like.

  Moreover, when detecting an ionic substance (especially one containing calcium ions) as a specific component, 4-acrylamidobenzo-18-crown-6-ether, acryloylaminobenzocrown ether, methacryloylaminobenzo as monomers. Crown ether group-containing monomers (particularly benzocrown ether group-containing monomers) such as crown ether and 4-vinylbenzocrown ether can be suitably used.

  When detecting an ionic substance such as sodium chloride as a specific component, as monomers, 3-acrylamidophenylboronic acid, vinylphenylboronic acid, acryloyloxyphenylboronic acid, N-isopropylacrylamide (NIPAAm), Ethylene bisacrylamide, N-hydroxyethyl acrylamide, etc. can be used suitably. In particular, when an ionic substance such as sodium chloride is detected as a specific component, the monomer is selected from the group consisting of 3-acrylamidophenylboronic acid, vinylphenylboronic acid, and acryloyloxyphenylboronic acid. Alternatively, two or more monomers are used in combination with one or more monomers selected from the group consisting of N-isopropylacrylamide (NIPAAm), ethylenebisacrylamide and N-hydroxyethylacrylamide. Is preferred.

  When lactic acid is detected as a specific component, monomers such as 3-acrylamidophenylboronic acid, vinylphenylboronic acid, acryloyloxyphenylboronic acid, N-isopropylacrylamide (NIPAAm), ethylenebisacrylamide, N -Hydroxyethyl acrylamide etc. can be used conveniently. In particular, when detecting lactic acid as a specific component, the monomer is one or more selected from the group consisting of 3-acrylamidophenylboronic acid, vinylphenylboronic acid and acryloyloxyphenylboronic acid. It is preferable to use a monomer in combination with one or more monomers selected from the group consisting of N-isopropylacrylamide (NIPAAm), ethylenebisacrylamide and N-hydroxyethylacrylamide.

  The polymerization initiator can be appropriately selected depending on, for example, the polymerization mode. Specific examples thereof include benzoyl peroxide, lauroyl peroxide, hydrogen peroxide, persulfates such as potassium persulfate and sodium persulfate. Azo initiators such as ammonium persulfate (for example, 2,2′-azobis (2-amidinopropane) dihydrochloride, azobisdimethylvaleronitrile, 2,2′-azobis (2-aminopropane) dihydrochloride, 2 , 2'-azobis [2- (5-methyl-2-imidazolin-2-yl) propane] dihydrochloride, 2,2'-azobisisobutyramide dihydrate, 2,2'-azobis [N- (2-Carboxyethyl) -2-methylpropionamidine] n-hydrate, 2,2′-azobis [2-methyl-N- (2-hydroxyethyl) propionami ], 2,2'-azobis (N, N'-dimethyleneisobutylamidine) dihydrochloride, 2,2'-azobis {2-methyl-N- [1,1, -bis (hydroxymethyl) -2- Hydroxyethyl] propionamide}, 2,2′-azobis [2- (2-imidazolin-2-yl) propane] dihydrochloride, 4,4′-azobis (4-cyanovaleric acid), 2,2′- Azobisisobutyronitrile, 2,2′-azobis (2,4′-dimethylvaleronitrile), etc.), benzophenone, 2,2-dimethoxy-1,2-diphenylethane-1-one, 1-hydroxycyclohexylphenyl Ketone, 2-hydroxy-2-methyl-1-phenylpropan-1-one, 2,4,6-trimethylbenzoyldiphenylphosphine oxide, 1- [4- (2-hydro Ciethoxy) -phenyl] -2-hydroxy-2-methyl-1-propan-1-one, 2,4-diethylthioxanthone, isopropylthioxanthone, 1-chloro-4-propoxythioxanthone, 2- (3-dimethylamino-2 -Hydroxypropoxy) -3,4-dimethyl-9H-thioxanthone-9-one mesochloride, 2-methyl-1 [4- (methylthio) phenyl] -2-morpholinopropane-1, 2-benzyl-2-dimethylamino -1- (4-morpholinophenyl) -butanone-1, bis (cyclopentadienyl) -bis (2,6-difluoro-3- (py-1-yl) titanium, diisopropyloxydicarbonate, 1,3- Di (t-butylperoxycarbonyl) benzene, 3,3 ′, 4,4′-tetra- (t- And peroxyesters such as butylperoxycarbonyl) benzophenone, t-butylperoxy-2-ethylhexanoate, t-butylperoxybivalate, and t-butylperoxydiisobutyrate. Hydrogen peroxide or persulfate can also be used as a redox initiator in combination with a reducing substance such as sulfite or L-ascorbic acid or an amine salt.

  The stimuli-responsive gel 2 preferably includes a polymer material (polymer) having a structure crosslinked with a crosslinking agent component.

  As a result, the heat resistance and the like of the polymer material (polymer) can be further improved. For example, the polymer material (from the container 3) under the more severe conditions (for example, in a high temperature environment) ( Leakage of the polymer) can be more suitably suppressed.

  Further, even when the stimulus-responsive gel 2 contains fine particles as described later, the fine particles can be suitably held in the stimulus-responsive gel 2 and the fine particles are more likely to leak out. It can be effectively suppressed.

  As the crosslinking agent component, a compound having two or more polymerizable functional groups can be used. Specifically, for example, ethylene glycol, propylene glycol, trimethylolpropane, glycerin, polyoxyethylene glycol, polyoxypropylene glycol , Polyglycerol, N, N′-methylenebisacrylamide, N, N-methylene-bis-N-vinylacetamide, N, N-butylene-bis-N-vinylacetamide, tolylene diisocyanate, hexamethylene diisocyanate, allylated starch Allylated cellulose, diallyl phthalate, tetraallyloxyethane, pentaerythritol triallyl ether, trimethylolpropane triallyl ether, diethylene glycol diallyl ether, triallyl trimellitate And the like, may be used alone or in combination of two or more selected from these.

  The stimulus-responsive gel 2 preferably includes at least a polymer (first polymer) including a water-soluble vinyl monomer as a constituent monomer and having a phenylboronic acid structure.

  Thereby, the change rate of the volume of the stimulus-responsive gel 2 due to the concentration change of the specific component can be increased, and the specific component can be quantitatively detected more suitably. In particular, detection of lactic acid or the like can be performed more suitably.

  Hereinafter, the case where the stimulus-responsive gel 2 includes at least a water-soluble vinyl monomer as a constituent monomer and a polymer having a phenylboronic acid structure (first polymer) will be mainly described.

  The phenylboronic acid structure may be introduced after polymerizing a polymerizable compound (monomer or the like) as a component of the polymer (first polymer), for example, but the polymer (first polymer) It is preferable that the polymerizable compound as a constituent component of In other words, the polymer (first polymer) preferably contains a monomer having a phenylboronic acid structure as a constituent component.

  Thereby, adjustment of the ratio etc. of the phenyl boronic acid structure which a polymer (1st polymer) has can be performed easily. Further, the rigidity of the polymer (first polymer) can be more easily performed. In addition, the chemical stability of the polymer (first polymer) can be made more excellent, and the unintended deterioration, modification, etc. of the polymer (first polymer) can be more effectively suppressed. .

  Examples of the monomer having a phenylboronic acid structure constituting the polymer (first polymer) include 3- (meth) acrylamide phenylboronic acid, vinylphenylboronic acid, (meth) acryloyloxyphenylboronic acid, (meta ) Acryloylaminobenzeneboronic acid, (meth) acryloylaminobenzeneboronic acid, 4-vinylbenzeneboronic acid and the like, and one or two or more selected from these can be used. The polymer (1) preferably contains 3-acrylamidophenylboronic acid as a constituent component.

  Thereby, the rate of change of the volume of the stimulus-responsive gel 2 due to the concentration change of the specific component can be further increased, and quantitative detection of the specific component can be more suitably performed.

  The polymer (first polymer) contains a water-soluble vinyl monomer (a water-soluble vinyl monomer having no phenylboronic acid structure) as a constituent monomer.

  Thereby, while the ratio of the phenylboronic acid structure which the said polymer (1st polymer) has can be adjusted to a suitable thing, the rate of change of the volume of the stimulus responsive gel 2 by the concentration change of a specific component is further It can be made large, and quantitative detection of a specific component can be performed more suitably.

The water-soluble vinyl monomer preferably has a solubility in 100 g of water at 25 ° C. of 5 g or more, more preferably 10 g or more.
Thereby, the effects as described above are more remarkably exhibited.

  The water-soluble vinyl monomer preferably has at least one of a hydroxyl group (OH group), a ketone group, a carboxylic acid group, and an ionic group, and has at least one of a hydroxyl group (OH group) and an ionic group. Those having a hydroxyl group (OH group) are more preferred.

  Thereby, the effects as described above are more remarkably exhibited. Moreover, the holding power of the solvent of the stimulus-responsive gel 2 (particularly, a solvent having a high polarity (hydrophilicity) such as water) can be further improved.

  In particular, when the water-soluble vinyl monomer has a hydroxyl group (OH group), the stimulus-responsive gel 2 has a first state in which a phenylboronic acid structure and an OH group are bonded, a phenylboronic acid structure and an OH group. And the second state in which the connection with is released.

  Such a change in state is caused by a change in the surrounding environment (the presence or absence of a specific component, a change in the concentration of the specific component, etc.). In particular, the change in the state as described above occurs in a region where the concentration of the specific component is relatively low. Therefore, the reactivity (sensitivity) with respect to the specific component of the stimulus responsive gel 2 can be made higher.

  Examples of water-soluble vinyl monomers (water-soluble vinyl monomers having no phenylboronic acid structure) constituting the polymer (first polymer) include N-hydroxyethyl (meth) acrylamide, diacetone (meth) acrylamide, N- Hydroxymethyl (meth) acrylamide, N-hydroxypropyl (meth) acrylamide, N-hydroxybutyl (meth) acrylamide, N-hydroxypentyl (meth) acrylamide, 2,3-dihydroxypropyl (meth) acrylamide, dihydroxybutyl (meth) Acrylamide, dihydroxypentyl (meth) acrylamide, tris (hydroxymethyl) methyl (meth) acrylamide, 2- (meth) acrylamide 2-methylsulfonic acid, (meth) acrylamide glycolic acid, (meth ) Acrylamide ethane carboxylic acid, (meth) acrylamide propane carboxylic acid, (meth) acrylamide butane carboxylic acid, (meth) acrylamide pentane carboxylic acid, (meth) acrylamide hexane carboxylic acid, (meth) acrylamide heptane carboxylic acid, (meth) acrylamide Octanecarboxylic acid, (meth) acrylamide ethoxyethanol, 2-hydroxy (meth) acrylate, 2-hydroxyethyl (meth) acrylate, (meth) acrylic acid monoglycerol, maleic anhydride glycerol mono (meth) acrylate, 2-hydroxy 1 -Acryloxy-3-meth (acryloxy) propane, 2-hydroxy 1,3-dimeth (acryloxy) propane, N, N-dimethylaminopropyl (meth) acrylamide Seed quaternary salt, N, N-dimethylaminoethyl (meth) acrylate, various quaternary salts, 1,4-cyclohexanedimethanol mono (meth) acrylate, (3- (meth) acrylamidopropyl) trimethylammonium chloride, trimethyl-2 -(Meth) acryloyloxyethylammonium chloride, 2-((meth) acryloyloxy) ethyl phosphate 2- (trimethylammonio) ethyl, 3-sulfopropylpotassium (meth) acrylate, 2- (meth) acrylamide 2 -Methylpropane sulfonic acid, N, N '-(1,2-dihydroxyethylene) bis (meth) acrylamide, vinyl sulfonic acid, etc. may be mentioned, and one or more selected from these may be used in combination. it can.

  Examples of the monomer having an OH group constituting the polymer (first polymer) include N-hydroxyethyl (meth) acrylamide, N-hydroxymethyl (meth) acrylamide, N-hydroxypropyl (meth) acrylamide, N -Hydroxybutyl (meth) acrylamide, N-hydroxypentyl (meth) acrylamide, 2,3-dihydroxypropyl (meth) acrylamide, dihydroxybutyl (meth) acrylamide, dihydroxypentyl (meth) acrylamide, tris (hydroxymethyl) methyl (meta ) Acrylamide, 2-hydroxy (meth) acrylate, 2-hydroxyethyl (meth) acrylate, 2-hydroxy 1-acryloxy-3-meth (acryloxy) propane, 2-hydroxy 1,3- Examples include meth (acryloxy) propane, N, N ′-(1,2-dihydroxyethylene) bis (meth) acrylamide, (meth) acrylamide ethoxyethanol, 1,4-cyclohexanedimethanol mono (meth) acrylate, and the like. The polymer (first polymer) preferably contains N-hydroxyethylacrylamide as a constituent component. However, the polymer (first polymer) can be used in combination.

  Thereby, the effects as described above are more remarkably exhibited. In addition, when the stimulus-responsive gel 2 includes the second polymer described in detail later in addition to the polymer (first polymer), the affinity between the first polymer and the second polymer is more suitable. The problem such as unintentional phase separation in the stimulus-responsive gel 2 can be more suitably suppressed over a longer period of time, and the durability and reliability of the stimulus-responsive gel 2 are particularly excellent. Can be.

  The content of the water-soluble vinyl monomer having no phenylboronic acid structure in the polymer (first polymer) is preferably 40 mol% or more and 80 mol% or less, and more preferably 50 mol% or more and 75 mol% or less. preferable.

  The content of the monomer having the phenylboronic acid structure in the polymer (first polymer) is preferably 20% by mass to 45% by mass, and more preferably 25% by mass to 43% by mass. preferable.

  Thereby, the softness | flexibility of the stimulus responsive gel 2 can be made more appropriate, and the sensitivity with respect to a specific component can be made especially excellent.

  On the other hand, if the content of the monomer having a phenylboronic acid structure in the polymer (first polymer) is less than the lower limit or exceeds the upper limit, the flexibility of the stimulus-responsive gel 2 In some cases, the sensitivity to a specific component may decrease.

  The polymer (first polymer) may contain a monomer other than the above-described monomers (monomers having a phenylboronic acid structure, water-soluble vinyl monomers having no phenylboronic acid structure) as constituent components. Good.

  Examples of such monomers include (meth) acrylamide, N-methyl (meth) acrylamide, N-ethyl (meth) acrylamide, N-isopropyl (meth) acrylamide, N, N-dimethyl (meth) acrylamide, N, N-dimethylaminopropyl (meth) acrylamide, ethylenebis (meth) acrylamide, N- [3- (dimethylamino) propyl] (meth) acrylamide, Nt-butyl (meth) acrylamide, N, N-diethyl (meth) ) Acrylamide, N- (butoxymethyl) (meth) acrylamide, N- (isobutoxymethyl) (meth) acrylamide, N-phenyl (meth) acrylamide, isobutoxymethyl (meth) acrylamide, N-vinyl lactone, methyl vinyl ether, (Meta) ak Roylmorpholine, (meth) acrylic acid, various alkyl (meth) acrylates, 1,4-cyclohexanedimethanol mono (meth) acrylate, (3- (meth) acrylamidopropyl) trimethylammonium chloride, trimethyl-2- (meth) acro Yloxyethylammonium chloride, 2-((meth) acryloyloxy) ethyl phosphate 2- (trimethylammonio) ethyl, 2- (2-ethoxyethoxy) ethyl (meth) acrylate, glycidyl (meth) acrylate, di (Ethylene glycol) divinyl ether, tri (ethylene glycol) divinyl ether, poly (ethylene glycol) divinyl ether, trimethylolpropane ethoxylate tri (meth) acrylate, trimethylolpropane propoxyler Tri (meth) acrylate, N- [tris (3- (meth) acrylamidepropoxymethyl) methyl] (meth) acrylamide, vinyl acetate, vinyl carboxylate (C3-C10), N-vinylpyrrolidone, acrylonitrile, styrene, polyethylene glycol Di (meth) acrylate, 1,6-hexanediol di (meth) acrylate, neopentyl glycol di (meth) acrylate, tripropylene glycol di (meth) acrylate, polypropylene glycol di (meth) acrylate, 2,2-bis [ 4-((meth) acryloxydiethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxypolyethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxy) Polypropoxy) phenyl] propa , Ethylene glycol di (meth) acrylate, diethylene glycol di (meth) acrylate, triethylene glycol di (meth) acrylate, 1,3-butylene glycol di (meth) acrylate, 2,2-bis [4-((meth)) Acryloxyethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxyethoxydiethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxyethoxypolyethoxy) phenyl] Propane, trimethylolpropane tri (meth) acrylate, tetramethylolmethane tri (meth) acrylate, tetramethylolmethane tetra (meth) acrylate, dipentaerythritol hexa (meth) acrylate, N, N'-methylenebis (meth) acrylamide, diety Glycol diallyl ether, divinylbenzene, 4- (meth) acrylamide benzo-18-crown-6-ether, acryloylaminobenzocrown ether, (meth) acryloylaminobenzocrown ether, 4-vinylbenzocrown ether, etc. One or more selected from these can be used in combination.

  Of the above monomers, acrylamide, methacrylamide, acrylic acid, and methacrylic acid are preferable, and acrylamide and methacrylamide are more preferable.

  The polymer (first polymer) preferably contains at least one of N-isopropylacrylamide (NIPAAm) and ethylenebisacrylamide.

  The content of the crosslinking agent component in the polymer (first polymer) is preferably 0.5 mol% or more and 10.0 mol% or less, more preferably 0.8 mol% or more and 8.0 mol% or less. 1.1 mol% or more and 7.0 mol% or less is more preferable.

  As a result, the degree of cross-linking of the polymer (first polymer) can be in a more suitable range, and the flexibility of the polymer (first polymer) can be improved while exhibiting the effects as described above more remarkably. It can be made more appropriate.

The stimulus-responsive gel 2 may include a plurality of different polymer materials.
For example, the stimulus-responsive gel 2 includes, as a polymer material, a water-soluble vinyl monomer as a constituent monomer as described above, and in addition to a first polymer having a phenylboronic acid structure, an OH group (to a carbon atom). It may contain a second polymer having a hydroxyl group to be bonded.

  Thereby, while the resin material which comprises the stimulus responsive gel 2 as mentioned above exhibits the effect more effectively by having a phenylboronic acid structure and an OH group, the strength of the stimulus responsive gel 2 is further increased. It can be made excellent, and the durability of the stimulus-responsive gel 2 and the biological component measuring apparatus 10 can be made more excellent.

  In addition, the stimulus-responsive gel 2 includes the second polymer having an OH group, so that the phenylboronic acid structure and the OH group are included even if the first polymer does not have an OH group. The first state that is bonded and the second state in which the bond between the phenylboronic acid structure and the OH group is released can be suitably taken, and even if the amount of change in the content of the specific component is small, the stimulus response The volume change of the gel 2 can be made larger, and the sensitivity to the specific component can be made more excellent. In addition, the holding power of the solvent of the stimuli-responsive gel 2 (particularly polar (hydrophilic) solvent such as water) can be made particularly excellent, and a suitable gel state can be stably maintained over a long period of time. Can do. In addition, when the first polymer has an OH group and further includes the second polymer, the effects described above are more remarkably exhibited.

  Hereinafter, the stimulus-responsive gel 2 has, as a resin material, an OH group (hydroxyl group bonded to a carbon atom) in addition to a polymer having a phenylboronic acid structure (first polymer), and has a phenylboronic acid structure. The case where the second polymer is not included will be mainly described.

(Second polymer)
The second polymer has an OH group (hydroxyl group).

  The OH group may be introduced after polymerizing a polymerizable compound (monomer or the like) as a polymer component, but is preferably contained in the polymerizable compound as a polymer component. .

  Thereby, adjustment of the ratio etc. of OH group which a 2nd polymer has can be performed easily.

  Examples of the monomer having an OH group constituting the second polymer include N-hydroxyethyl (meth) acrylamide, N-hydroxymethyl (meth) acrylamide, N-hydroxypropyl (meth) acrylamide, N-hydroxybutyl ( (Meth) acrylamide, N-hydroxypentyl (meth) acrylamide, 2,3-dihydroxypropyl (meth) acrylamide, dihydroxybutyl (meth) acrylamide, dihydroxypentyl (meth) acrylamide, tris (hydroxymethyl) methyl (meth) acrylamide, 2 -Hydroxy (meth) acrylate, 2-hydroxyethyl (meth) acrylate, 2-hydroxy 1-acryloxy-3-meth (acryloxy) propane, 2-hydroxy 1,3-dimethacrylate (acrylo) Si) Propane, N, N ′-(1,2-dihydroxyethylene) bis (meth) acrylamide, (meth) acrylamide ethoxyethanol, 1,4-cyclohexanedimethanol mono (meth) acrylate, and the like are selected. However, it is preferable that the second polymer contains N-hydroxyethylacrylamide as a constituent component.

  Thereby, the stimulus-responsive gel 2 more suitably has a first state in which the phenylboronic acid structure and the OH group are bonded, and a second state in which the bond between the phenylboronic acid structure and the OH group is released. Even if the amount of change in the content of the specific component is small, the volume change of the stimulus-responsive gel 2 can be increased, and the sensitivity to the specific component can be further improved. . In addition, the holding power of the solvent of the stimuli-responsive gel 2 (particularly polar (hydrophilic) solvent such as water) can be made particularly excellent, and a suitable gel state can be stably maintained over a long period of time. Can do.

  Further, the second polymer may include a monomer having no OH group as its constituent component. Thereby, the ratio of the OH group which the 2nd polymer has, etc. can be adjusted to a suitable thing.

  Examples of the monomer having no OH group constituting the second polymer include (meth) acrylamide, N-methyl (meth) acrylamide, N-ethyl (meth) acrylamide, N-isopropyl (meth) acrylamide, N , N-dimethyl (meth) acrylamide, N, N-dimethylaminopropyl (meth) acrylamide, ethylenebis (meth) acrylamide, N- [3- (dimethylamino) propyl] (meth) acrylamide, diacetone (meth) acrylamide, N-t-butyl (meth) acrylamide, N, N-diethyl (meth) acrylamide, N- (butoxymethyl) (meth) acrylamide, N- (isobutoxymethyl) (meth) acrylamide, N-phenyl (meth) acrylamide 2- (meth) acrylamide-2 Methylsulfonic acid, (meth) acrylamide glycolic acid, isobutoxymethyl (meth) acrylamide, (meth) acrylamide ethanecarboxylic acid, (meth) acrylamide propanecarboxylic acid, (meth) acrylamide butanecarboxylic acid, (meth) acrylamide pentanecarboxylic acid , (Meth) acrylamide hexane carboxylic acid, (meth) acrylamide heptane carboxylic acid, (meth) acrylamide octane carboxylic acid, (meth) acrylamide ethoxyethanol, N-vinyl lactone, (meth) acrylic acid monoglycerol, methyl vinyl ether, maleic anhydride Glycerol mono (meth) acrylate, N, N-dimethylaminopropyl (meth) acrylamide quaternary salts, (meth) acryloylmorpholine, N, N-dimethyl Various amino acid (meth) acrylate quaternary salts, (meth) acrylic acid, various alkyl (meth) acrylates, 1,4-cyclohexanedimethanol mono (meth) acrylate, (3- (meth) acrylamidopropyl) trimethylammonium chloride, trimethyl 2- (meth) acryloyloxyethylammonium chloride, 2-((meth) acryloyloxy) ethyl phosphate 2- (trimethylammonio) ethyl, 3-sulfopropyl potassium (meth) acrylate, 2- (meth) Acrylamide-2-methylpropanesulfonic acid, 2- (2-ethoxyethoxy) ethyl (meth) acrylate, glycidyl (meth) acrylate, di (ethylene glycol) divinyl ether, tri (ethylene glycol) divinyl ether, poly (ethylene) Glycol) divinyl ether, trimethylolpropane ethoxylate tri (meth) acrylate, trimethylolpropane propoxylate tri (meth) acrylate, N- [tris (3- (meth) acrylamidepropoxymethyl) methyl] (meth) acrylamide, vinyl Sulfonic acid, vinyl acetate, vinyl carboxylate (C3-C10), N-vinylpyrrolidone, acrylonitrile, styrene, polyethylene glycol di (meth) acrylate, 1,6-hexanediol di (meth) acrylate, neopentyl glycol di (meta ) Acrylate, tripropylene glycol di (meth) acrylate, polypropylene glycol di (meth) acrylate, 2,2-bis [4-((meth) acryloxydiethoxy) phenyl] propa 2,2-bis [4-((meth) acryloxypolyethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxypolypropoxy) phenyl] propane, ethylene glycol di (meth) acrylate , Diethylene glycol di (meth) acrylate, triethylene glycol di (meth) acrylate, 1,3-butylene glycol di (meth) acrylate, 2,2-bis [4-((meth) acryloxyethoxy) phenyl] propane, 2 , 2-bis [4-((meth) acryloxyethoxydiethoxy) phenyl] propane, 2,2-bis [4-((meth) acryloxyethoxypolyethoxy) phenyl] propane, trimethylolpropane tri (meth) Acrylate, tetramethylol methane tri (meth) acrylate, Lamethylolmethane tetra (meth) acrylate, dipentaerythritol hexa (meth) acrylate, N, N′-methylenebis (meth) acrylamide, diethylene glycol diallyl ether, divinylbenzene, 4- (meth) acrylamide benzo-18-crown-6 Examples include ether, acryloylaminobenzocrown ether, (meth) acryloylaminobenzocrown ether, 4-vinylbenzocrown ether, and the like, and one or more selected from these can be used in combination.

  The content of the monomer having no OH group in the second polymer is preferably 0.1 mol% or more and 40 mol% or less, more preferably 0.2 mol% or more and 30 mol% or less, and 0.3 mol% % To 20 mol% is more preferable.

  The content of the crosslinking agent component in the second polymer is preferably 0.5 mol% or more and 7.0 mol% or less, more preferably 0.8 mol% or more and 6.0 mol% or less, and 1.1 mol % To 5.0 mol% is more preferable.

  As a result, the degree of cross-linking of the second polymer can be in a more suitable range, and the flexibility of the second polymer can be made more appropriate while exhibiting the effects as described above more remarkably. it can.

  The content of the monomer having an OH group in the second polymer is preferably 54 mol% or more and 99 mol% or less, more preferably 65 mol% or more and 98.5 mol% or less, and 76 mol% or more and 98 mol% or less. More preferably.

  As a result, the effects of the components other than the monomer having an OH group (crosslinking agent, monomer having no OH group, etc.) are exhibited while the effect of the second polymer having the OH group is more remarkably exhibited. Can be fully exhibited.

  The hydroxyl value of the second polymer is preferably 15 mgKOH / g or more and 620 mgKOH / g or less, more preferably 34 mgKOH / g or more and 78 mgKOH / g or less.

  Thereby, the durability of the stimuli-responsive gel 2 can be made particularly excellent while the effect of the second polymer having an OH group as described above more remarkably exhibited.

The content X2 of the _second polymer in the stimulus-responsive gel 2 is preferably 0.05% by mass or more and 98% by mass or less, and more preferably 0.1% by mass or more and 70% by mass or less. .

  Thereby, while the resin material which comprises the stimulus responsive gel 2 as mentioned above exhibits the effect more effectively by having a phenylboronic acid structure and an OH group, the strength of the stimulus responsive gel 2 is further increased. It can be made excellent, and the durability of the stimulus-responsive gel 2 and the biological component measuring apparatus 10 can be made more excellent.

  The content of the second polymer in the polymer material is preferably 1.0% by mass or more and 99% by mass or less, and more preferably 1.5% by mass or more and 98% by mass or less.

  Thereby, while the resin material which comprises the stimulus responsive gel 2 as mentioned above exhibits the effect more effectively by having a phenylboronic acid structure and an OH group, the strength of the stimulus responsive gel 2 is further increased. It can be made excellent, and the durability of the stimulus-responsive gel 2 and the biological component measuring apparatus 10 can be made more excellent.

The content X ₁ of the first polymer in the stimulus-responsive gel 2 is preferably 0.01% by mass or more and 70% by mass or less, and more preferably 0.05% by mass or more and 65% by mass or less. .

  Thereby, the softness | flexibility of the stimulus responsive gel 2 can be made more suitable, and the sensitivity with respect to a specific component can be made more excellent.

  In addition, the content of the first polymer in the polymer material is preferably 1.0% by mass or more and 70% by mass or less, and more preferably 2.0% by mass or more and 65% by mass or less.

  Thereby, the softness | flexibility of the stimulus responsive gel 2 can be made more suitable, and the sensitivity with respect to a specific component can be made more excellent.

When the content of the first polymer in the stimulus-responsive gel 2 is X ₁ [% by mass] and the content of the second polymer is X ₂ [% by mass], 0.2 ≦ X ₁ / X ₂ ≦ It is preferable that the relationship of 8 is satisfied, and it is more preferable that the relationship of 1.3 ≦ X ₁ / X ₂ ≦ 1.9 is satisfied.

  Thereby, the softness | flexibility of the stimulus responsive gel 2 can be made more appropriate, and the sensitivity with respect to a specific component can be made more excellent.

  The stimulus-responsive gel 2 may include a polymer (polymer material) other than the first polymer and the second polymer described above. For example, a polymer containing neither a phenylboronic acid structure nor an OH group in the molecule may be included.

  The content of the polymer material in the stimulus-responsive gel 2 is preferably 0.7% by mass or more and 36.0% by mass or less, and more preferably 2.4% by mass or more and 27.0% by mass or less. preferable.

  When the stimulus-responsive gel 2 contains a plurality of types of polymers, at least one type of polymer has a network structure, and the polymer chain of the other polymer has the network structure. It is preferable to penetrate into the mesh.

  Thereby, durability, heat resistance, etc. of the stimulus responsive gel 2 can be made more excellent, and the polymer material (from the housing part 3) even under more severe conditions (for example, in a high temperature environment) Leakage of the polymer) can be more suitably suppressed.

  Further, even when the stimulus-responsive gel 2 contains fine particles as described later, the fine particles can be suitably held in the stimulus-responsive gel 2 and the fine particles are more likely to leak out. It can suppress suitably.

  In particular, it includes a first polymer having a phenylboronic acid structure and a second polymer having an OH group and a network structure, and the polymer chain of the first polymer enters the network of the second polymer. Is more preferable.

  Thereby, while being able to acquire the effect as mentioned above, the reactivity (sensitivity) with respect to the specific component of the stimulus responsive gel 2 can be made higher.

  In addition, when a local deformation of the stimulus-responsive gel 2 occurs due to the polymer chain of the first polymer entering the network of the second polymer, the deformation is efficiently propagated to the surroundings, As a result, since the entire stimulus responsive gel 2 is deformed with high uniformity, variations in structural color at each site of the stimulus responsive gel 2 are also suppressed. As a result, the concentration of the predetermined component can be easily and accurately determined.

  In addition, the OH group included in the second polymer and the phenylboron structure included in the first polymer have high reactivity, and can take a first state in which they are bonded. The structure in which the polymer chain of the first polymer enters the network is not easily broken and is stably held for a long period of time. Therefore, the predetermined stimulus can be detected stably over a long period of time. That is, the durability of the stimulus-responsive gel 2 is more excellent.

  In addition to the second polymer, if the first polymer has a network structure (crosslinked structure), the following effects can be obtained.

  That is, the first polymer can be more preferably prevented from dropping from the second polymer network, and the state in which the first polymer enters the second polymer network is stable for a longer period of time. Retained. This is because the closed polymer structure formed by the second polymer and the closed ring structure formed by the second polymer form a chain by the network structure (a strong structure formed by covalent bonds) of the first polymer. This is because the relationship becomes like a ring next to each other.

  As a result, the state in which the first polymer and the second polymer are close to each other can be stably maintained, and the concentration of the predetermined component can be detected more stably over a long period of time.

  Moreover, the retention power of the solvent of the stimulus-responsive gel 2 can be made particularly excellent, and a suitable gel state can be stably maintained over a long period of time.

  Whether or not the first polymer enters the network of the second polymer in the stimulus-responsive gel 2 can be confirmed by observation with a microscope, for example. It can also be determined by measuring the melting point (for example, measuring the endothermic peak of the melting point by differential scanning calorimetry (DSC)). More specifically, when the first polymer and the second polymer are independent and have different melting points, the melting point peak of the first polymer and the second polymer are measured in the melting point measurement. A melting point peak different from the melting point peak of (the first polymer and the second polymer have a structure that is microscopically integrated by entering the second polymer network) In the case where the melting point peak is confirmed, it can be determined that the first polymer has entered the network of the second polymer.

Moreover, it can also be confirmed whether the 1st polymer has penetrated into the network of a 2nd polymer using the difference in solubility of a 1st polymer and a 2nd polymer. More specifically, for example, for the stimulus-responsive gel 2, an organic solvent that dissolves only one of the first polymer and the second polymer (for example, 25 ° C. of one of the first polymer and the second polymer). The solubility at 25 ° C. of the other of the first polymer and the second polymer (amount that can be dissolved in 100 g of solvent) is 1 g / 100 g or more. In the presence of 0.01 g / 100 g or less), the organic solvent was added at 25 ° C. so that the amount added to the stimulus-responsive gel: 10 g was 1000 g, and further stirred sufficiently at this temperature. Later, filtration was performed, and the theoretical total amount of the extract (the total amount of the extract assuming that the solvent was not volatilized during filtration) was 200 times that of the stimulus-responsive gel used. The first polymer and the second polymer contained in the stimulus-responsive gel mixed with the organic solvent contained in the extract when the residue is washed with the organic solvent so as to satisfy (mass basis) Of the component soluble in the organic solvent among the first polymer and the first polymer contained in the extract with respect to the content of the component soluble in the organic solvent (W ₁ [g]) ( If it is confirmed that the ratio of W ₂ [g]) ((W ₂ / W ₁ ) · 100) is a predetermined value or less (preferably 10% by mass or less, more preferably 1% by mass or less) It can be determined that the first polymer enters the network of the two polymers.

  It is also possible to confirm whether or not the first polymer has entered the network of the second polymer by observing the structure of the first polymer and the second polymer under a microscope. More specifically, for example, the stimulus-responsive gel is cleaved in a state frozen with liquid nitrogen or a cross-sectional sample of the stimulus-responsive gel is prepared with a cryo-FIB and observed with a cryo SEM. At that time, if no island-like structure or grain is observed, it can be determined that the first polymer has entered the network of the second polymer. Furthermore, by performing mapping analysis on carbon, oxygen, and boron elements using an energy dispersive X-ray spectrometer (EDX), more detailed observation is possible, and the first polymer is in the network of the second polymer. It can be judged that it has entered.

  The network structure (crosslinked structure) as described above can be suitably introduced by using a crosslinking agent component as a constituent component of the polymer.

<Solvent>
When the stimulus-responsive gel 2 contains a solvent, the above-described polymer material can be suitably gelled.

  As the solvent, various organic solvents and inorganic solvents can be used. More specifically, for example, water; various alcohols such as methanol and ethanol; ketones such as acetone; ethers such as tetrahydrofuran and diethyl ether; dimethylformamide Amides such as: Chain aliphatic hydrocarbons such as n-pentane, n-hexane, n-heptane and n-octane; Cycloaliphatic hydrocarbons such as cyclohexane and methylcyclohexane; Aromatics such as benzene, toluene and xylene In particular, those containing water are preferable.

The stimulus-responsive gel 2 may include a plurality of different components as solvents.
The content of the solvent in the stimulus-responsive gel 2 is preferably 30% by mass or more and 95% by mass or less, and more preferably 50% by mass or more and 90% by mass or less.

<Particle>
The stimulus-responsive gel 2 may include, for example, particles (fine particles) having an average particle diameter of 10 nm to 1000 nm.

  Thereby, the structural color by Bragg reflection can be recognized. In particular, as the stimulus-responsive gel 2 expands or contracts, the distance between the adjacent fine particles changes, and the structural color (wavelength of reflected light) due to Bragg reflection also changes, so that a specific component is optically (visually) included. Can be detected. Further, since the structural color or the structural color change due to the Bragg reflection is recognized, the specific component can be quantified more easily and more accurately by the color tone, for example. Moreover, even if it does not use the detection apparatus for detecting the deformation | transformation (expansion or shrinkage | contraction) of the stimulus responsive gel 2, a specific component can be detected and quantified suitably by visual observation. Even in the case of using a detection device, it is only necessary to detect a change in color tone rather than a detection of a minute shape change, so that a relatively inexpensive detection device can be used.

When the stimuli-responsive gel 2 includes particles, the average particle size of the particles is preferably 10 nm or more and 1000 nm or less, more preferably 20 nm or more and 500 nm or less.
Thereby, the effects as described above are more remarkably exhibited.

  In the present specification, the average particle diameter means a volume-based average particle diameter. For example, a dispersion obtained by adding a sample to methanol and dispersing for 3 minutes with an ultrasonic disperser is used as a Coulter counter particle size distribution analyzer (COULTER). It can be determined by measuring with a 50 μm aperture using ELECTRONICS INC TA-II type).

  Fine particles are composed of inorganic materials such as silica and titanium oxide; polystyrene, polyester, polyimide, polyolefin, poly (meth) acrylate methyl, polyethylene, polypropylene, polyethersulfone, nylon, polyurethane, polyvinyl chloride, polychlorinated Examples thereof include organic materials (polymers) such as vinylidene, and the fine particles are preferably silica fine particles.

  Thereby, the stability of the shape of the fine particles can be made particularly excellent, and the durability and reliability of the stimulus-responsive gel 2 and the biological component measuring apparatus 10 can be made particularly excellent. Silica fine particles are relatively easy to obtain as those having a sharp particle size distribution (monodispersed fine particles), which is advantageous from the viewpoint of stable production and supply of the stimulus-responsive gel 2 and the biological component measuring apparatus 10. It is.

  The shape of the fine particles is not particularly limited, but is preferably spherical. Thereby, visual recognition of the structural color by a colloidal crystal can be performed easily, and a specific component can be quantified more easily.

The stimulus-responsive gel 2 may include a plurality of different types of fine particles.
The content of the fine particles in the stimulus-responsive gel 2 is preferably 1.6% by mass or more and 36% by mass or less, and more preferably 4.0% by mass or more and 24% by mass or less.

  Thereby, the effects as described above due to the fact that the stimulus-responsive gel 2 contains fine particles are more remarkably exhibited.

<Conductive substance>
The stimulus-responsive gel 2 may include, for example, a conductive substance.

  Thereby, according to the expansion-contraction state of the stimulus-responsive gel 2, the distance between the conductive substances contained in the stimulus-responsive gel 2 can be made different. As a result, the resistance of the stimulus-responsive gel 2 The value (resistivity) can be different. That is, when the stimulus-responsive gel 2 is in an expanded state (or in a state where the degree of expansion is large), the distance between the conductive substances (in a state where the stimulus-responsive gel 2 is contracted (or in a state where the degree of expansion is small)) ( Since the average distance) increases, the resistance value (resistivity) of the stimulus-responsive gel 2 increases. Therefore, by determining the resistance value (resistivity) of the stimulus-responsive gel 2, it is possible to easily and stably determine the expansion / contraction state of the stimulus-responsive gel 2, that is, the intensity of stimulation (the concentration of a specific component). Can do.

  When the stimulus-responsive gel 2 includes a conductive substance, the conductive substance may be included in any form, for example, may be included in a dissolved state or the like, but included as particles. Preferably.

  Thereby, the range of selection of a conductive substance is expanded. Moreover, the change rate of the resistance value (resistivity) of the stimulus-responsive gel 2 due to the expansion and contraction state of the stimulus-responsive gel 2 can be made more stable, and the detection accuracy can be made more excellent. Can do.

  Examples of the conductive substance contained in the stimulus-responsive gel 2 include metal materials (for example, Au, Ag, Pt, Cu and alloys containing at least one of these), conductive metal oxides (for example, ITO, etc.), carbon materials (eg, carbon black, etc.), conductive polymer materials (eg, PEDOT / PSS, polythiophene, polyacetylene, etc.) and the like.

  By including such a conductive substance, the rate of change in the resistance value accompanying the expansion or contraction of the stimulus-responsive gel 2 is improved while the durability of the stimulus-responsive gel 2 and the biological component measuring apparatus 10 is excellent. The detection accuracy of the stimulus can be further improved. Moreover, since these materials are comparatively cheap and can be obtained stably, they are advantageous from the viewpoint of suppressing production cost and stable supply of the stimulus-responsive gel 2 and the biological component measuring apparatus 10.

In particular, when the conductive substance contained in the stimulus-responsive gel 2 is a transparent material such as ITO,
The identification of the structural color involving the particles as described above (particles contributing to the expression of the structural color) is facilitated, and stimulation can be detected more suitably.

The electrical resistivity of the conductive substance contained in the stimulus-responsive gel 2 is preferably 1.0 × 10 ⁻³ Ω · m or less, and preferably 1.0 × 10 ⁻⁴ Ω · m or less. More preferably, it is 5.0 × 10 ⁻⁵ Ω · m or less.

  Thereby, the change rate of the said resistance value accompanying the expansion | swelling or shrinkage | contraction of the stimulus responsive gel 2 can be made larger, and the detection accuracy of a stimulus can be made more excellent.

  The refractive index of the conductive substance contained in the stimulus-responsive gel 2 is preferably 1.0 or more and 1.8 or less, and more preferably 1.1 or more and 1.7 or less.

  Thereby, the visibility (easiness of identification) of the structural color by Bragg reflection involving the fine particles (particles contributing to the expression of the structural color) as described above can be further improved.

  When the particle | grains comprised with the material containing an electroconductive substance are included, the said particle | grain may have a uniform composition in each site | part, and may have a site | part of a different composition.

  For example, the particles (particles made of a material containing a conductive substance) may have a core portion and at least one layer of coating provided on the outer surface side of the core portion.

In the case of including particles composed of a material containing a conductive substance, the particles may have any shape such as spherical shape, spindle shape, needle shape (spine shape), plate shape (scale shape), red blood cell like disk shape, etc. It may be present, but is preferably spherical.
Thereby, the reliability of the detected electrical resistivity becomes more excellent.

  When particles composed of a material containing a conductive substance are included, the average particle size of the particles is not particularly limited, but is preferably 10 nm or more and 1000 μm or less, and more preferably 20 nm or more and 500 μm or less.

  Thereby, the said particle | grain can be disperse | distributed more uniformly in the stimulus responsive gel 2, and the detection accuracy of a stimulus can be made more excellent.

<Other ingredients>
The stimulus-responsive gel 2 may contain components other than those described above (other components).

  Examples of such components include colorants, slip agents (leveling agents), antifungal agents, preservatives, antioxidants, humectants, and the like.

  The stimulus-responsive gel 2 may react with any component (specific component) as long as it is a component contained in a body fluid, but preferably reacts with stimulation by lactic acid as a specific component.

  The stimuli-responsive gel 2 as described above can be detected with excellent quantitative properties, particularly lactic acid, among various specific components, and can be quantified in a wide concentration range from a low concentration to a high concentration. It is possible to detect lactic acid.

  Lactic acid is a substance produced in the living body, and is a chemical substance (specific component) that can be suitably used for evaluating the state of the living body.

  In particular, lactic acid is a substance that increases in production due to a decrease in blood flow to tissues during severe shock conditions such as heart disease and sepsis, lack of oxygen in tissues, and increased glycolytic metabolism. . For this reason, treatment at the time of shock can be appropriately performed by detecting lactic acid as a specific component.

  The sepsis guideline also recommends measurement of blood lactate as an index for treatment. Conventionally, arterial blood is mainly collected to measure blood lactate, and blood gas analyzers are used to measure lactate. Was measuring. In such a method, it was practically impossible to continuously monitor lactic acid level fluctuations in the patient's body at the patient's bedside. Moreover, conventionally, when measuring a lactic acid level, an enzyme (enzyme electrode) is usually used. However, the enzyme is a bioreagent, and is expensive, and has a problem that it easily deteriorates due to storage environment such as temperature and humidity, and easily affects quantitativeness. In addition, since the activity of an enzyme is likely to vary depending on the production lot, manufacturer, storage environment, etc., it is necessary to calibrate the enzyme using a standard solution having a known concentration before using the enzyme electrode. Furthermore, since oxygen is usually required for enzyme reactions, in hypoxic conditions such as shock conditions, there is a high possibility that the oxygen concentration in the body tissue has decreased, and there is an error in the quantification results of lactic acid levels by enzymes. There was also a problem that it was likely to occur.

  On the other hand, in the biological component measuring apparatus 10, since it is not necessary to use an enzyme, the above problems can be prevented from occurring.

[Second Embodiment]
Next, the biological component measuring apparatus of 2nd Embodiment is demonstrated.
FIG. 2 is a schematic cross-sectional view for explaining a second embodiment of the biological component measuring apparatus. In the following description, differences from the above-described embodiment will be mainly described, and description of similar matters will be omitted.

  In the biological component measuring apparatus 10 of the present embodiment, the collection unit 1 includes a hollow member 12 having flexibility.

  Thus, the collection part 1 is not limited to what was comprised with the material with high rigidity, and may be comprised with the material which has flexibility.

  With such a configuration, the length of the collection unit 1 to be inserted (for example, punctured) into the living body can be made longer. In addition, the safety of the biological component measuring apparatus 10 can be made higher.

  Examples of the hollow member 12 include a hollow fiber, a porous tube, and the like (particularly, microdialysis using these materials).

  By providing such a hollow member 12, body fluid can be collected more efficiently. Moreover, the safety | security with respect to a biological body can be made more excellent.

  The hollow fiber is a hollow fiber that has pores having a size according to the purpose on a straw-like fiber wall surface, and can exchange substances inside and outside the hollow fiber.

  The constituent material of the hollow fiber membrane is not particularly limited. For example, polysulfone, a polysulfone polymer such as a copolymer of polysulfone and polyvinylpyrrolidone, ethylene vinyl alcohol, polyacrylonitrile, polyethylene, regenerated cellulose, cellulose diacetate, Examples thereof include cellulose triacetate, polyethersulfone, polymethyl methacrylate and the like.

  As a porous tube, for example, a fluororesin such as tetrafluoroethylene-hexafluoropropylene copolymer (FEP) and PTFE, a tube of water repellent resin such as silicone and polypropylene is stretched in the major axis direction, a carbon dioxide laser, etc. Those having a micropore (hole) formed by applying the laser beam can be suitably used.

  The porous tube refers to a tube (tube) having fine pores formed on its wall surface, and the outside and inside (hollow part) of the porous tube communicate with each other when the pores communicate with each other. doing.

  The size of the micropore is not particularly limited, but the diameter is preferably 1 nm or more and 200 μm or less, and more preferably 2 nm or more and 100 μm or less.

  In addition, the biological component measurement apparatus 10 of the present embodiment includes a collection auxiliary liquid supply unit 5 that supplies a collection auxiliary liquid (flow liquid) that assists the collection of body fluid into the living body.

  By supplying the collection auxiliary liquid from the collection auxiliary liquid supply unit 5 in the living body, the body fluid to be collected can be efficiently guided to the collection unit 1, and the detection efficiency of the specific component should be more excellent. Can do. In particular, the specific component can be suitably detected when the specific component to be detected has low fluidity in a normal living body (in a living body in a state where the collection auxiliary liquid is not supplied).

  The body fluid collected by the collection unit 1 may be diluted with a collection auxiliary liquid. Even in such a case, it is possible to determine whether or not the specific component is contained in the collected body fluid. Further, the supply speed of the collection auxiliary liquid supplied from the collection auxiliary liquid supply section 5, the collection speed of the body fluid collected by the collection section 1, the part in the living body to which the collection auxiliary liquid is supplied, and the sex of the collection section 1 By using parameters such as the installation site in the body, it is possible to obtain the dilution rate of the body fluid collected by the collection unit 1 with the collection auxiliary liquid, and the inclusion of the predetermined component in a state where it is not diluted with the collection auxiliary liquid The rate can be determined appropriately.

  Further, when the body fluid collected by the collection unit 1 is diluted with the collection auxiliary liquid, the following effects can be obtained. That is, when the stimulus-responsive gel 2 is sensitive to the specific component to be detected and the volume change rate is saturated even at a relatively low concentration, the specific component to be detected is diluted in the living body. Thus, quantitative detection of the specific component can be performed more suitably.

  The collection assisting liquid supply unit 5 may be anything as long as it can supply the collection assisting liquid for assisting the collection of the body fluid into the living body, for example, a pressure higher than the atmospheric pressure in the container. The sample may be filled with a collection auxiliary liquid (flow liquid), or may be equipped with a pump such as a micropump.

  Since the collection auxiliary liquid supply unit 5 includes a pump, the collection auxiliary liquid can be more efficiently supplied into the living body over a long period of time.

  As the collection auxiliary liquid (flow liquid), for example, an electrolyte solution such as physiological saline or Ringer's solution, a non-electrolyte solution such as a glucose aqueous solution, or the like can be suitably used. In addition, as the collection auxiliary liquid (flow liquid), a liquid in which an electrolyte and a non-electrolyte coexist may be used.

  The osmotic pressure concentration (osmolarity) of the collection auxiliary liquid (flow liquid) is not particularly limited, but is preferably 0.20 [Osm / L] or more and 0.40 [Osm / L] or less, and is 0.25 [ Osm / L] or more and 0.35 [Osm / L] or less is more preferable.

  Thereby, generation | occurrence | production of problems, such as hemolysis at the time of introduce | transducing a collection assistance liquid (flow liquid) in the biological body, can be suppressed, and a specific component can be detected more suitably.

In the illustrated configuration, the collection auxiliary liquid supply unit 5 is connected to the collection unit 1.
Thereby, a body fluid (in particular, a body fluid diluted with a collection auxiliary liquid) can be collected more efficiently.

  Moreover, in this embodiment, the biological component measuring apparatus 10 is provided with the decompression means 4 which makes the space in the accommodating part 3 a negative pressure together with the collection auxiliary liquid supply part 5.

  Thereby, the above-described effects can be obtained, and the collection auxiliary liquid can be efficiently introduced into the living body from the collection auxiliary liquid supply unit 5. In addition, the body fluid recovery rate can be improved. In addition, it is possible to more effectively suppress the collection auxiliary liquid from unintentionally diffusing into the living body, and the detection accuracy and reliability when detecting a specific component quantitatively are improved. It can be.

  In particular, when the decompression means 4 such as a pump (first pump) and the collection auxiliary liquid supply unit 5 have a pump (second pump), these functions work cooperatively, For example, the collection speed and collection rate of body fluid can be further improved, and the collection auxiliary liquid can be more effectively suppressed from unintentionally diffusing into the living body. The accuracy and reliability of detection when detecting a specific component can be further improved.

<< Use of biological component measuring device >>
Next, the use of the biological component measuring apparatus 10 will be described with specific examples.

  Since the biological component measuring apparatus 10 can easily detect a specific component contained in a body fluid, for example, a device that checks whether or not a specific component is contained in a body fluid that is a specimen, or a body fluid that is a specimen It can be used to measure the concentration of a specific component contained therein.

  In particular, the present invention can be applied to body fluid monitoring, determination of tolerance to a load during exercise, physical condition management, etc. for a patient with a predetermined disease.

  In addition, since the biological component measuring apparatus 10 can easily detect the amount of the specific component taken into the stimulus-responsive gel 2, it is used as a separation / extraction means for separating / extracting the specific component contained in the body fluid. You can also That is, when the amount of the specific component taken into the stimulus-responsive gel 2 is saturated or is likely to saturate, contact with the body fluid that is the specimen is stopped and replaced with another biological component measuring device 10 as necessary. can do. Thereby, a specific component can be collect | recovered from a bodily fluid without waste.

<< Usage method of biological component measuring apparatus >>
Next, the usage method of a biological component measuring apparatus is demonstrated.
FIG. 3 is a flowchart showing a preferred embodiment of a method for using the biological component measuring apparatus.

  As shown in FIG. 3, the method of using the biological component measuring apparatus 10 of the present embodiment includes an installation process in which at least a part of the collection unit 1 is installed at a desired site in the living body, and a body fluid collected from the collection unit 1. And a detection step of detecting a specific component contained in the bodily fluid by bringing the bodily fluid into contact with the stimuli-responsive gel 2.

<Installation process>
In the installation step, at least a part of the collection unit 1 is installed at a desired site in the living body.

  In this step, any method may be used. For example, it can be performed by surgery, but is usually performed by puncture.

  In addition, when the stimulus-responsive gel 2 includes a conductive substance as described above, a part to be an electrode of the stimulus-responsive gel 2 is electrically connected in advance to an electrical resistance measurement unit, and stimulation by a specific component is performed. Calibration may be performed by measuring (initializing) the resistance value in a state in which it has not been received. At this time, for example, calibration may be performed using a standard solution containing a specific component at a predetermined concentration. By performing the above configuration, the detection accuracy of the specific component can be further increased.

<Detection process>
In the detection step, a component contained in the body fluid is detected by bringing the collected body fluid into contact with the stimulus-responsive gel 2 in the storage unit 3.

  Thus, unlike the conventional blood test, it is not necessary to separately perform a test such as a biochemical test after collecting a body fluid, and a specific component can be detected in real time.

  Data relating to measurement results obtained using the biological component measurement apparatus 10 may be stored by, for example, a storage unit.

  Thereby, for example, fluctuations in the amount of the specific component over time can be suitably managed. In addition, for example, by managing data on a plurality of patients, a statistical tendency or the like can be found, and can be effectively used for determining a treatment policy for a specific disease.

  Moreover, you may make it link the data regarding the measurement result obtained using the biological component measuring apparatus 10 with a hospital system or an electronic medical chart, for example.

As mentioned above, although preferred embodiment was described, it is not limited to these.
For example, the biological component measurement device may have a configuration other than that described above.

  For example, the biological component measurement device may include a light source that emits light irradiated to the stimulus-responsive gel. Thereby, the further improvement of the detection sensitivity and detection accuracy of a specific component can be aimed at.

  In addition, the biological component measuring apparatus may be configured such that a part of the constituent members is configured to be replaceable, or configured to be removable and remountable. For example, the collection part or stimulus-responsive gel punctured in the living body may be configured to be replaceable or removable and remountable.

  In the above-described embodiment, the stimulus-responsive gel (stimulus-responsive gel material) is representatively described as being in a gel state even in the state before the use of the biological component measurement device. The responsive gel material only needs to be in a gel state when the biological component measuring device is used. For example, the responsive gel material is not in a gel state during storage of the biological component measuring device (for example, in a dry state). ). In such a case, for example, prior to the use of the biological component measuring device, a gel or the like can be formed by applying a liquid or the like that constitutes the solvent of the gel material to the stimulus-responsive gel material.

  In the second embodiment described above, the collection auxiliary liquid supply unit is described as being connected to the collection unit. However, the collection auxiliary liquid supply unit and the collection unit may be used separately. Good.

  DESCRIPTION OF SYMBOLS 10 ... Biological component measuring device, 1 ... Collecting part, 11 ... Needle-like body, 111 ... Through-hole (hollow part), 12 ... Hollow member, 2 ... Stimulus responsive gel, 3 ... Accommodating part, 4 ... Decompression means, 5 ... Collecting auxiliary liquid supply unit

Claims (16)

A collection unit for indwelling and collecting body fluid;
A stimulus-responsive gel that expands or contracts by stimulation with a specific component contained in the body fluid;
While containing the stimulus-responsive gel, and a storage unit connected to the collection unit,
A biological component measuring apparatus comprising: The sampling part includes a needle-like body having a through hole,
The living body component measuring apparatus according to claim 1, wherein the through hole communicates with the housing portion.   The living body component measuring apparatus according to claim 2, wherein the stimulus-responsive gel is disposed at a position separated from the through hole.   The biological component measuring apparatus according to claim 3, wherein the collection unit includes a plurality of the needle-like bodies.   The biological component measuring apparatus according to any one of claims 1 to 4, further comprising decompression means for making a negative pressure in the space in the housing portion.   The biological component measuring apparatus according to claim 1, wherein the collection unit includes a hollow member having flexibility.   The living body component measuring apparatus according to claim 6, comprising a hollow fiber or a porous tube as the hollow member.   The biological component measuring device according to any one of claims 1 to 7, further comprising a collection assisting liquid supply unit that supplies a collection assisting liquid for assisting the collection of the body fluid into the living body.   The biological component measuring device according to claim 8, wherein the collection auxiliary liquid supply unit is connected to the collection unit.   The living body component measuring apparatus according to claim 1, wherein the stimulus-responsive gel is responsive to stimulation by lactic acid as the specific component.   The living body component measuring apparatus according to any one of claims 1 to 10, wherein the stimulus-responsive gel includes a water-soluble vinyl monomer as a constituent monomer and a polymer having a phenylboronic acid structure.   The biological component measuring apparatus according to claim 11, wherein the polymer includes a monomer having a phenylboronic acid structure as a constituent component.   The biological component measuring device according to claim 11 or 12, wherein the polymer contains N-hydroxyethylacrylamide as the water-soluble vinyl monomer. The stimulus-responsive gel includes a first polymer having a phenylboronic acid structure, and a second polymer having an OH group and a network structure,
The biological component measuring apparatus according to any one of claims 1 to 13, wherein a polymer chain of the first polymer enters a network of the second polymer.   The living body component measuring apparatus according to any one of claims 1 to 14, wherein the stimulus-responsive gel is formed by dispersing particles having an average particle diameter of 10 nm or more and 1000 nm or less.   The living body component measuring apparatus according to claim 1, wherein the stimulus-responsive gel contains a conductive substance.

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