JP2017053702A - Minute channel device and measuring method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a minute channel device and a measuring method that keep cost down.SOLUTION: A minute channel device 100 comprises a minute channel where fluid flows through and is used when materials included in the fluid are measured by surface enhanced Raman spectroscopy. The minute channel device comprises: a pipe member 110 where a hollow part 111 extending along a prescribed direction is formed; and a fitting member 120 fitted into the hollow part 111. An outer peripheral part 120a of the fitting member 120 is provided with a minute groove 121 that forms a minute channel between an inner peripheral part 110a of the pipe member 110 and the outer peripheral part, and metal nanoparticles M for surface enhanced Raman spectroscopy are formed on the groove internal surface of the minute groove 121.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a microchannel device and a measuring method that are provided with a microchannel through which a fluid flows, and that are used when a substance contained in the fluid is measured by surface-enhanced Raman spectroscopy.

  Conventionally, in the field of chromatography typified by high performance liquid chromatography (HPLC), it is important to identify a substance contained in a fluid, and surface enhanced Raman spectroscopy (SERS) is a method for identifying this substance. : Surface Enhanced Raman Scattering) (see, for example, Patent Document 1).

  In surface-enhanced Raman spectroscopy, metal nanoparticles for surface-enhanced Raman spectroscopy (hereinafter, appropriately described as metal nanoparticles) formed on the inner surface of a channel carrying fluid are irradiated with excitation light. The Raman scattered light emitted from is measured. And a substance can be identified by analyzing the measured Raman scattered light.

  Here, when a substance contained in a fluid is measured by surface-enhanced Raman spectroscopy, the substance contained in the fluid comes into contact with metal nanoparticles formed on the inner surface of the flow path when the diameter of the flow path for transporting the fluid is large. Therefore, a technique using a microchannel device in which a microchannel having a channel diameter of about 100 μm is formed has been studied (for example, see Patent Document 1).

JP2013-142546A Japanese Patent Laying-Open No. 2015-014512

  However, the prior art has the following problems. The microchannel device according to the prior art is manufactured through a processing process in which fine grooves are formed inside a transparent substrate and metal nanoparticles are formed on the groove inner surface of the fine grooves. Such a fine channel device that requires fine and special processing has been very expensive. For this reason, there has been a demand for a fine channel device with reduced cost.

  This invention is made | formed in view of such a condition, and it aims at providing the microchannel device and measuring method which suppressed cost.

  A first feature of the present invention is a microchannel device that includes a microchannel for flowing a fluid, and is used when measuring a substance contained in the fluid by surface-enhanced Raman spectroscopy, along a predetermined direction. A tube member in which an extending hollow portion is formed; and a fitting member fitted into the hollow portion; at least a Raman spectroscopic measurement portion of the tube member covering the fitting member is a transparent member. A fine groove is formed on the outer peripheral portion of the fitting member to form the fine flow channel between the outer peripheral portion of the fitting member and the inner peripheral portion of the pipe member. The gist is that metal nanoparticles for Raman spectroscopy are formed.

  The second feature of the present invention relates to the above feature, and is summarized in that the tube member is a hollow tube.

  A third feature of the present invention is related to the above feature, and is summarized in that the fine groove is formed in a spiral shape on an outer peripheral portion of the fitting member.

  A fourth feature of the present invention relates to the above feature, wherein the cross-sectional shape of the fine groove is any one of a V shape, a U shape, a U shape, or a combination thereof. .

  A fifth feature of the present invention is the above feature, wherein the fitting member further includes a minute member fixed to a groove bottom of the minute groove, and the metal nanoparticles are formed on an outer surface of the minute member. The gist is that

  A sixth feature of the present invention includes a step of measuring a substance contained in the fluid by surface-enhanced Raman spectroscopy using a microchannel device including a microchannel that allows fluid to flow, and the microchannel device includes: A tube member in which a hollow portion extending along a predetermined direction is formed, and a fitting member fitted into the hollow portion, and at least a Raman spectroscopic measurement portion of the tube member covering the fitting member Is formed of a transparent member, and the outer periphery of the fitting member is provided with a minute groove that forms the minute channel between the inner periphery of the tube member, and the groove of the minute groove The gist is that metal nanoparticles for surface enhanced Raman spectroscopy are formed on the inner surface.

  ADVANTAGE OF THE INVENTION According to this invention, the microchannel device and measuring method which suppressed cost can be provided.

1 is a schematic configuration diagram illustrating a configuration of a measurement system according to a first embodiment of the present invention. It is an expanded sectional view of the fine channel device concerning a 1st embodiment of the present invention. It is an expanded sectional view of the fine channel device concerning a 1st embodiment of the present invention. It is a flowchart which shows the measuring method which concerns on 1st Embodiment of this invention. It is a graph which shows an example of the Raman spectrum measured by the measuring system concerning a 1st embodiment of the present invention. (A) It is explanatory drawing which shows the fitting member which concerns on the modification of this invention. (B) It is explanatory drawing which shows the fitting member which concerns on the modification of this invention. (A) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (B) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (A) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (B) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (C) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (A) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention. (B) It is explanatory drawing which shows the other example of the fitting member which concerns on 1st Embodiment of this invention.

  Hereinafter, a first embodiment of the present invention will be described with reference to the drawings.

[First embodiment of the present invention]
(Configuration of measurement system 1)
FIG. 1 is a schematic configuration diagram of a measurement system 1 according to the first embodiment of the present invention. The measurement system 1 according to the first embodiment of the present invention identifies a substance contained in a fluid by surface-enhanced Raman spectroscopy in a flow system.

  As shown in FIG. 1, the measurement system 1 according to the first embodiment includes a feeder 10, transport pipes 20 and 30, a fine channel device 100, a light emitter 40, and a light receiver 50.

  The supply machine 10 supplies a fluid containing a substance to be a specimen as a part of the flow system. The conveyance pipes 20 and 30 are hollow pipes and convey a fluid. The microchannel device 100 is disposed between the transport pipes 20 and 30 so as to connect the transport pipes 20 and 30.

  The light emitter 40 irradiates the microchannel device 100 with the excitation light L1, and the light receiver 50 receives the Raman scattered light L2 emitted by the excitation light L1. The Raman scattered light L2 received by the light receiver 50 is analyzed as a Raman spectrum. The measurement system 1 also includes other devices (not shown) such as a spectroscope and an analysis device, but detailed description thereof is omitted here.

(Configuration of microchannel device 100)
Next, the configuration of the fine channel device 100 will be described. FIG. 2 is a cross-sectional view taken along the central axis Ax of the microchannel device 100. In the present embodiment, as shown in FIG. 2, an X direction along the central axis Ax and a Y direction orthogonal to the central axis Ax are defined. FIG. 3 is an enlarged cross-sectional view in which a part of the microchannel device 100 in FIG. 2 is enlarged.

  The microchannel device 100 includes a microchannel that allows a fluid to flow, and is used when a substance contained in the fluid is measured by surface-enhanced Raman spectroscopy.

  As shown in FIG. 2, the microchannel device 100 includes a tube member 110 in which a hollow portion 111 extending along a predetermined direction is formed, and a fitting member 120 fitted in the hollow portion 111. That is, the pipe member 110 covers the fitting member 120. The predetermined direction is a fluid conveyance direction, and in the present embodiment, the predetermined direction is the X direction along the central axis Ax of the microchannel device 100.

In the present embodiment, at least the Raman spectroscopic measurement portion 110X of the tube member 110 is formed of a transparent member. The Raman spectroscopic measurement portion 110X is a portion through which the excitation light L1 and the scattered light L2 are transmitted. The size of the Raman spectroscopic measurement portion 110X is not particularly limited as long as it can irradiate the excitation light L1 and can receive the Raman scattered light L2. For example, the size of the Raman spectroscopic measurement portion 110X may be 2 mm × 2 mm or more (4 mm ² or more) or 2 mm or more in diameter when viewed along the Y direction orthogonal to the central axis Ax. Of course, the entire tube member 110 may be formed of a transparent member.

  The transparent member that is the material of the Raman spectroscopic measurement portion 110X of the tube member 110 is generally a transparent member at the wavelength used for surface-enhanced Raman spectroscopy. For example, the tube member 110 may be made of glass, a resin such as plastic or silicone, and any other member. In the present embodiment, “transparent” means that the transmittance is 50% or more for light of a specific wavelength in a wavelength band of 380 nm to 2000 nm. The light transmittance of the Raman spectroscopic measurement portion 110X of the tube member 110 is more preferably 80% or more. Further, the light transmittance of the Raman spectroscopic measurement portion 110X of the tube member 110 may be adjusted by increasing or decreasing its thickness.

  In the present embodiment, the pipe member 110 is a hollow tube. For example, the tube member 110 is a cylindrical hollow tube made of an elastic member such as silicone.

  The fitting member 120 is fitted into the hollow portion 111 of the tube member 110. A fine groove 121 that forms a fine flow path between the outer peripheral portion 120 a of the fitting member 120 and the inner peripheral portion 110 a of the tube member 110 is provided.

  Specifically, the outer end portion 123 formed by the fine groove 121 is in contact with the inner peripheral portion 110 a of the pipe member 110, so that a fine flow path is formed in the gap space between the fine groove 121 and the pipe member 110. . It can also be said that the fine groove 121 constitutes a fine flow path. The fine groove 121 continues from one side of the fitting member 120 in the X direction to the other side. Thereby, the fine channel communicates with both sides of the fitting member 120 in the X direction.

  In the present embodiment, the fine groove 121 is spirally formed in the outer peripheral portion 120 a of the fitting member 120. Specifically, the fine groove 121 extends in a spiral shape around the central axis Ax in the outer peripheral portion 120a.

As shown in FIG. 3, the cross-sectional shape of the fine groove 121 is V-shaped in the direction orthogonal to the extending direction of the fine groove 121. The groove width Wg of the fine groove 121 is preferably in the range of 10 μm to 1 mm. The groove depth Dg of the fine groove 121 is preferably in the range of 10 μm to 100 μm. Moreover, in the direction orthogonal to the extending direction of the fine groove 121, the cross-sectional area of the fine groove 121 is preferably in the range of 100 μm ^{2 to} 0.1 mm ² .

  Here, it is preferable to use the screw (so-called male screw) prescribed | regulated to a specification for the fitting member 120 from a viewpoint of comprising more cheaply. The standard is determined by an industrial standard effective in the region where it is used, and is, for example, a standard defined by JIS (Japanese Industrial Standards) or ISO (International Organization for Standardization).

  For example, in the microchannel device 100, when the tube member 110 having an inner diameter D110 of 3 mm is used, an M3 screw may be used for the fitting member 120. When an elastic member is used as the material of the tube member 110, the tube member 110 can be extended in the Y direction orthogonal to the central axis Ax. For this reason, the diameter D120 of the fitting member 120 may be, for example, about 5 to 10% larger than the inner diameter D110 of the tube member 110.

  Materials such as steel, special steel, stainless steel, aluminum, titanium, and resin can be applied to the material of the fitting member 120.

  The fitting member 120 is fixed in a state of being fitted into the hollow portion 111 of the tube member 110. In the present embodiment, the fitting member 120 is fixed to the hollow portion 111 of the tube member 110 by being screwed into the hollow portion 111 of the tube member 110.

  On the groove inner surface 122 of the fine groove 121, surface-enhanced Raman spectroscopic metal nanoparticles M (hereinafter, appropriately described as metal nanoparticles M) are formed. The metal which comprises the metal nanoparticle M is noble metals, such as gold | metal | money, silver, platinum, for example. Moreover, as a method for forming the metal nanoparticles M, for example, the following method can be applied.

  First, silica nanoparticles having a particle size of 50 to 500 nm are adsorbed on the groove inner surface 122 of the fitting member 120. The method of adsorbing silica nanoparticles can be appropriately performed depending on the material of the fitting member 120. For example, after forming a gold thin film on the groove inner surface 122 of the fitting member 120 by vacuum deposition or plating, the silica nanoparticles are adsorbed. You may let them. Thereby, silica nanoparticles can be easily adsorbed.

  Next, gold or silver having a thickness of 5 to 500 nm is coated on a part of the silica nanoparticles in a cap shape by vacuum deposition, thereby forming noble metal cap-shaped nanoparticles on the groove inner surface 122 of the fitting member 120.

  In the above example, the method of forming noble metal cap-shaped nanoparticles as the metal nanoparticles M is described as an example, but the present invention is not limited to this. For example, the metal nanoparticles M may be formed by adsorbing the nanoparticles themselves made of a noble metal to the groove inner surface 122 of the fitting member 120.

  Further, the fitting member 120 in which the metal nanoparticles M are formed as described above is fitted into the hollow portion 111 of the pipe member 110, whereby a fine flow is formed in the gap space between the fine groove 121 and the pipe member 110. The microchannel device 100 in which the path is formed can be manufactured.

(Measuring method)
Next, a measurement method using the measurement system 1 will be described. FIG. 4 is a flowchart showing the measurement method.

  In step S10, the measurement system 1 is prepared. Specifically, the transport pipe 20, the fine channel device 100, and the transport pipe 30 are connected to the feeder 10.

  In step S20, the substance contained in the fluid is measured by the surface enhanced Raman spectroscopy using the microchannel device 100. Specifically, in the measurement system 1, the fluid supplied from the supply machine 10 is transported via the transport pipe 20, the fine channel device 100, and the transport pipe 30.

  At this time, in the microchannel device 100, the fluid spirally flows on the outer peripheral portion 120 a of the fitting member 120 along the microgroove 121 provided in the fitting member 120.

  And when the excitation light L1 radiated | emitted from the light emitter 40 is irradiated to the metal nanoparticle M formed in the microchannel device 100 via the tube member 110, the Raman scattered light L2 is received by the light receiver 50. Detected. The Raman scattered light L2 detected by the light receiver 50 is detected as a Raman spectrum by a spectroscope (not shown). In the measurement system 1, the substance contained in the fluid is identified by analyzing the detected Raman spectrum.

(Function and effect)
As described above, the microchannel device 100 according to the first embodiment of the present invention includes the tube member 110 and the fitting member 120. A fine groove 121 is formed in the outer peripheral portion 120 a of the fitting member 120 so as to form a fine flow passage with the inner peripheral portion 110 a of the tube member 110. Further, metal nanoparticles M for surface enhanced Raman spectroscopy are formed on the groove inner surface 122 of the fine groove 121.

  That is, in the microchannel device 100, the tube member 110 and the fitting member 120 are separate bodies, and the metal nanoparticles M are formed on the groove inner surface 122 of the microgroove 121 of the fitting member 120.

  According to the fine channel device 100, if the fitting member 120 is fitted in the hollow portion 111 of the tube member 110, a fine channel in which the metal nanoparticles M are formed on the groove inner surface 122 can be easily formed. This eliminates the need for special processing such as the formation of fine grooves inside the substrate and the formation of metal nanoparticles on the inner surfaces of the fine grooves, as in the prior art. The microchannel device 100 can be manufactured at low cost.

  Thus, according to the microchannel device 100 according to the present embodiment, the microchannel device 100 with reduced cost can be provided.

  Moreover, the pipe member generally used when flowing a fluid can be applied to the pipe member 110. For example, the transport pipe 20 and the transport pipe 30 can be applied to the pipe member 110. For this reason, the cost at the time of manufacturing the microchannel device 100 can be suppressed further.

  Furthermore, if the fitting member 120 is fitted into the hollow portion 111 of the pipe member 110, a fine flow path can be formed inside the pipe member 110. Thereby, compared with the case where the plate-like substrate and other pipe members such as the transfer pipe 20 and the transfer pipe 30 are connected as in the prior art, it is possible to connect without gaps, so that fluid leaks at the connection portion. Problems such as occurrence can be prevented. Moreover, it becomes easy to incorporate the micro-channel device 100 into a part of the flow system for flowing a fluid, and it becomes easy to construct the flow system.

  In the present embodiment, the fine groove 121 is spirally formed in the outer peripheral portion 120 a of the fitting member 120. Thereby, it becomes easy to screw the fitting member 120 into the hollow portion 111 of the tube member 110. That is, since the fitting member 120 can be easily attached to the tube member 110, the microchannel device 100 is easily manufactured.

[Example]
Next, examples of the present invention will be described. In addition, this invention is not limited at all by these examples.

  Here, in the embodiment, the measurement system 1 is used to measure the first state before flowing the fluid to the microchannel device 100 and the second state while the fluid is flowing to the microchannel device 100. Each measurement result was to be compared.

  First, as the microchannel device 100 in the first state, the microchannel device 100 having the fitting member 120 to which the reference model molecule was attached was created. As a reference model molecule, trans 1,2 bis 4-pyridylethylene (common name, BPE) was used. Specifically, the fitting member 120 was immersed in a 1 mM solution of BPE, and then the fitting member 120 was washed and dried. Moreover, the fine channel device 100 was created by fitting the fitting member 120 after drying to the pipe member 110.

  Then, the microchannel device 100 in the first state was measured by surface enhanced Raman spectroscopy.

  Next, the microchannel device 100 in the second state was measured while flowing a fluid containing the target model molecule through the microchannel device 100 in the first state. Specifically, a 1 mM solution of p-aminothiophenol (common name, PATP), which is a target model molecule, was measured in real time by surface-enhanced Raman spectroscopy while flowing as a fluid.

  FIG. 5 shows a graph showing the measurement results measured using the measurement system 1. The vertical axis in FIG. 5 indicates the Raman signal intensity, and the horizontal axis indicates the Raman shift (cm−1).

  In FIG. 5, data X <b> 1 is data obtained by measuring a Raman spectrum using the microchannel device 100 in the first state in which a reference model molecule is attached without flowing a fluid through the microchannel device 100.

  On the other hand, in FIG. 5, data X2 is data obtained by measuring a Raman spectrum in real time using the microchannel device 100 in the second state in which a 1 mM solution of PATP as a target model molecule is flowed as a fluid.

  As shown in FIG. 5, the peak observed in the data X1 is decreased in the data X2, and the change of the peak between the data X1 and X2 can be confirmed. In particular, in data X2, since a peak around 1073 cm −1, which is a characteristic of PATP, can be detected, it has been confirmed that substances contained in the fluid can be measured in real time.

[Modification]
Next, a modification of the first embodiment described above will be described. In 1st Embodiment mentioned above, although the metal nanoparticle M was formed in the groove | channel inner surface 122 of the fitting member 120, it is not limited to this. For example, the fitting member 120 may further include a micro member 132 that is fixed to the groove bottom 122 b of the micro groove 121, and the metal nanoparticles M may be formed on the surface 132 a of the micro member 132.

  Specifically, as shown in FIG. 6A, the micro member 132 is fixed to the groove bottom 122 b of the micro groove 121 by being embedded in a hole 131 formed in a part of the micro groove 121. Also good. A plurality of minute members 132 may be arranged in a part of the minute groove 121 with a predetermined interval.

  The minute member 132 may be configured to penetrate the fitting member 120. The micro member 132 may be fixed using an adhesive or may be fixed by tightening the male screw formed on the micro member 132 into the female screw formed on the hole 131. .

  The shape of the micro member 132 may be any column shape such as a cylinder, a quadrangular column, or a triangular column. Moreover, the shape of the micro member 132 may be spherical as shown in FIG.

  Further, the minute member 132 may have a string shape. In this case, the string-like minute member 132 is arranged in a state of being fixed along the groove bottom portion 122 b of the minute groove 121.

  As described above, the fitting member 120 may be configured to be attached to the minute groove 121 after the minute member 132 in which the metal nanoparticles M are formed is separately formed.

  Accordingly, the metal nanoparticles M can be more reliably formed on the minute member 132 and then attached to the minute groove 121, so that the fitting member 120 in which the metal nanoparticles M are more reliably formed can be created. Therefore, the Raman scattered light L2 can be more reliably emitted by the measurement method using the surface enhanced Raman spectroscopy, and as a result, the measurement accuracy can be improved.

[Other Embodiments of the Present Invention]
Although the present invention has been described in detail using the above-described embodiments, it is obvious to those skilled in the art that the present invention is not limited to the embodiments described in this specification.

  For example, in the first embodiment described above, the case where the cross-sectional shape of the fine groove 121 is V-shaped has been described as an example, but is not limited thereto. For example, as shown in FIGS. 7A to 7B, the cross-sectional shape of the fine groove 121 may be a U-shape or a U-shape. That is, the cross-sectional shape of the fine groove 121 may be any of a V shape, a U shape, and a U shape. Alternatively, a combination thereof may be used. That is, the structure which combined 2 or more of V shape, U shape, and U shape may be sufficient.

  For example, when the cross-sectional shape of the fine groove 121 is a U-shape or a U-shape, the bottom surface of the groove bottom portion 122b can be secured wider than the V-shape, and the metal nanoparticles M are formed on the bottom surface. it can. For this reason, the excitation light L1 emitted from the light emitter 40 is easily applied to the metal nanoparticles M, and the Raman scattered light L2 is more easily emitted.

  Various shapes can be applied to the fitting member 120 and the fine groove 121. For example, as shown in FIG. 8A, even when the fitting member 120 has a columnar shape and the fine groove 121 extending along the central axis Ax is formed on the outer peripheral portion 120 a of the fitting member 120. Good. Further, as shown in FIG. 8B, even when the fitting member 120 has a conical shape and the fine groove 121 extending along the central axis Ax is formed in the outer peripheral portion 120 a of the fitting member 120. Good. Further, as shown in FIG. 8C, the fitting member 120 has an oval shape, and a fine groove 121 extending along the central axis Ax is formed in the outer peripheral portion 120 a of the fitting member 120. Also good.

Or as shown to Fig.9 (a), the fitting member 120 is a cylinder shape which has the hollow part 125, Comprising: Both ends are connected to the hollow part 125 in some outer peripheral parts 120a of the fitting member 120. As shown in FIG. The structure by which the fine groove | channel 121 is formed may be sufficient. Further, as shown in FIG. 9B, the fine groove 121 formed on the outer peripheral portion 120a of the fitting member 120 includes a first fine groove 121a having a groove cross-sectional area of a predetermined area and a groove cross-sectional area of a predetermined area. May be constituted by a small second fine groove 121b. Incidentally, groove cross-sectional area of the first fine groove 121a ^is 0.01 mm 2 to 10 mm ^2, groove cross-sectional area of the second fine groove 121b is preferably 100μm ² ~0.1mm ^2.

  According to the fitting member 120 shown in FIGS. 9A to 9B, the fine groove 121 and the second fine groove 121 b having a small groove area are formed only in a part of the outer peripheral portion 120 a of the fitting member 120. Compared with the case where it forms all over, the channel resistance at the time of conveying a fluid can be controlled.

  For example, in the above-mentioned embodiment, in the hollow part 111 of the pipe member 110, although the fitting member 120 was fixed by being screwed by fitting, it is not limited to this. For example, the fitting member 120 may be fixed to the hollow portion 111 of the tube member 110 with an adhesive. Alternatively, the fitting member 120 may be fixed by further including a sandwiching member that sandwiches the pipe member 110 and the fitting member 120 from the outside of the pipe member 110.

  Further, for example, from the viewpoint of improving the working efficiency of fitting the fitting member 120 to the pipe member 110, the fitting member 120 is screwed with a tool such as a screwdriver at the end portion (end surface) in the X direction of the fitting member 120. Therefore, a minus (−) groove or a plus (+) groove may be formed.

  As described above, the present invention is not limited to the above-described embodiments as they are, and can be embodied by modifying the constituent elements without departing from the scope of the invention in the implementation stage. Various inventions can be formed by appropriately combining a plurality of constituent elements disclosed in the embodiments. For example, some components may be deleted from all the components shown in the embodiment.

  The fine channel device and the measurement method of the present invention can be applied to real-time monitoring of a substance contained in a fluid.

DESCRIPTION OF SYMBOLS 1 ... Measurement system 10 ... Supply machine 20,30 ... Conveyance pipe 100 ... Fine flow path device 110 ... Pipe member 110a ... Inner peripheral part 111 ... Hollow part 120 ... Fitting member 120a ... Outer peripheral part 121 ... Fine groove 122 ... Groove inner surface 122b ... groove bottom part 123 ... outer end part 125 ... hollow part 131 ... hole part 132 ... minute member M ... metal nanoparticles

Claims (6)

A microchannel device comprising a microchannel for flowing a fluid, and used when measuring a substance contained in the fluid by surface-enhanced Raman spectroscopy,
A tube member formed with a hollow portion extending along a predetermined direction;
A fitting member fitted into the hollow part,
At least the Raman spectroscopic measurement part of the tube member covering the fitting member is formed of a transparent member,
The outer periphery of the fitting member is provided with a minute groove that forms the minute channel between the inner periphery of the tube member,
A microchannel device, wherein metal nanoparticles for surface enhanced Raman spectroscopy are formed on the inner surface of the microgroove. The microchannel device according to claim 1, wherein the tube member is a hollow tube. The fine channel device according to claim 1, wherein the fine groove is formed in a spiral shape on an outer peripheral portion of the fitting member. 4. The fine structure according to claim 1, wherein a cross-sectional shape of the fine groove is any one of a V shape, a U shape, a U shape, or a combination thereof. 5. Channel device. The fitting member further includes a micro member fixed to a groove bottom of the micro groove,
5. The microchannel device according to claim 1, wherein the metal nanoparticles are formed on an outer surface of the micro member. Measuring a substance contained in the fluid by surface enhanced Raman spectroscopy, using a microchannel device including a microchannel for flowing a fluid;
The fine channel device is:
A tube member formed with a hollow portion extending along a predetermined direction;
A fitting member fitted into the hollow part,
At least the Raman spectroscopic measurement part of the tube member covering the fitting member is formed of a transparent member,
A fine groove that forms the fine flow path is formed between the outer peripheral portion of the fitting member and the inner peripheral portion of the pipe member,
A measurement method, wherein metal nanoparticles for surface-enhanced Raman spectroscopy are formed on the inner surface of the fine groove.

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