JP2016516394A5 - - Google Patents
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- JP2016516394A5 JP2016516394A5 JP2015548081A JP2015548081A JP2016516394A5 JP 2016516394 A5 JP2016516394 A5 JP 2016516394A5 JP 2015548081 A JP2015548081 A JP 2015548081A JP 2015548081 A JP2015548081 A JP 2015548081A JP 2016516394 A5 JP2016516394 A5 JP 2016516394A5
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- JP
- Japan
- Prior art keywords
- seq
- primer
- reverse primer
- forward primer
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- Prior art date
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- LVTKHGUGBGNBPL-UHFFFAOYSA-N 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole Chemical compound N1C2=CC=CC=C2C2=C1C(C)=C(N)N=C2C LVTKHGUGBGNBPL-UHFFFAOYSA-N 0.000 description 1
- 102000003425 EC 1.14.18.1 Human genes 0.000 description 1
- 108060008724 EC 1.14.18.1 Proteins 0.000 description 1
- 102000013760 Microphthalmia-Associated Transcription Factor Human genes 0.000 description 1
- 108010050345 Microphthalmia-Associated Transcription Factor Proteins 0.000 description 1
- 102100007096 TYRP1 Human genes 0.000 description 1
- 108010051081 dopachrome isomerase Proteins 0.000 description 1
- 108010010447 trophoblastin Proteins 0.000 description 1
Description
プライマーを次のように設計した：ＫＩＴフォワードプライマー、５’−ＧＡＡＡＧＴＧＡＣＧＴＣＴＧＧＴＣＣＴＡＴＧＧ−３’（配列番号１）；リバースプライマー、５’−ＧＴＧＣＴＣＴＣＴＧＡＡＡＴＣＴＧＣＴＴＣＴＣＡ−３’（配列番号２）；ドーパクローム異性化酵素（ＤＣＴ）フォワードプライマー、５’−ＴＣＣＴＴＣＣＴＧＡＡＣＧＧＧＡＣＡＡＡ−３’（配列番号３）；リバースプライマー、５’−ＴＧＧＣＡＴＡＧＣＴＧＴＡＧＣＣＡＡＧＴＴＧ−３’（配列番号４）。ＭＩＴＦフォワードプライマー、５’−ＣＧＧＧＡＡＣＡＧＧＡＣＣＡＴＧＧＴＴＡ−３’（配列番号５）；リバースプライマー、５’−ＡＧＣＴＡＧＣＣＣＣＴＧＡＡＡＴＧＡＡＴＣＣ−３’（配列番号６）；ｇｐ１００フォワードプライマー、５’−ＣＣＡＧＴＧＴＡＴＣＣＣＣＡＧＧＡＡＡＣＴＧ−３’（配列番号７）；リバースプライマー、５’−ＧＡＡＴＧＡＧＣＡＡＧＡＧＧＣＡＣＡＴＡＧＣＴ−３’（配列番号８）；ＴＲＰ−１、フォワードプライマー、５’−ＴＧＣＡＣＡＣＣＴＴＣＡＣＡＧＡＴＧＣＡ−３’（配列番号９）；リバースプライマー、５’−ＡＡＧＣＧＣＣＡＡＣＴＡＣＴＧＣＴＡＴＧＧ−３’（配列番号１０）；チロシナーゼフォワードプライマー、５’−ＧＡＧＧＴＣＡＧＣＡＣＣＣＣＡＣＡＡＡＴ−３’（配列番号１１）；リバースプライマー、５’−ＧＣＡＧＣＴＴＴＡＴＣＣＡＴＧＧＡＡＣＣＡ−３’（配列番号１２）。 Primers were designed as follows: KIT forward primer, 5′-GAAAGTGACCGTCTGGTCCTATGGG-3 ′ (SEQ ID NO: 1) ; reverse primer, 5′-GTGCTCTCTGAAATCTGCTTCTCCA-3 ′ (SEQ ID NO: 2) ; dopachrome isomerase (DCT) Forward primer, 5′-TCCTTCCTGAACGGGACAAA-3 ′ (SEQ ID NO: 3) ; reverse primer, 5′-TGGCATAGCTGTCCCAAGTTG-3 ′ (SEQ ID NO: 4) . MITF forward primer, 5′-CGGGAACAGGACCATGGGTTA-3 ′ (SEQ ID NO: 5) ; reverse primer, 5′-AGCTAGCCCCCTGAAATGAATCC-3 ′ (SEQ ID NO: 6) ; gp100 forward primer, 5′-CCAGGTATCCCCAGGAAACTG-3 ′ (SEQ ID NO: 7) ; Reverse primer, 5′-GAATGAGCAAGAGCCACATAGCT-3 ′ (SEQ ID NO: 8) ; TRP-1, forward primer, 5′-TGCACACCTCCAGATAGGC-3 ′ (SEQ ID NO: 9) ; Reverse primer, 5′-AAGCGCCAACTACTGCCTTAGGG-3 ′ (SEQ ID NO: 10) ); tyrosinase forward primer, 5'-GAGGTCAGCACCCCACAAAT-3 '(SEQ ID 11); reverse primer, 5'-GCAGCTTTATCCATGGAACCA-3 '(SEQ ID NO: 12).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361807465P | 2013-04-02 | 2013-04-02 | |
| US61/807,465 | 2013-04-02 | ||
| PCT/JP2014/060045 WO2014163206A1 (en) | 2013-04-02 | 2014-04-01 | Use of functional melanocytes readily differentiated from multilineage-differentiating stress-enduring (Muse) cells, distinct stem cells in human fibroblasts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016516394A JP2016516394A (en) | 2016-06-09 |
| JP2016516394A5 true JP2016516394A5 (en) | 2016-12-08 |
Family
ID=51658491
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015548081A Pending JP2016516394A (en) | 2013-04-02 | 2014-04-01 | Use of functional melanocytes easily differentiated from Muse cells, characteristic stem cells in human fibroblasts |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2016516394A (en) |
| WO (1) | WO2014163206A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107530378A (en) * | 2014-11-18 | 2018-01-02 | 宾夕法尼亚州立大学托管会 | By the composition and method that directly reprogram generation melanocyte |
| GB201510913D0 (en) * | 2015-06-22 | 2015-08-05 | Nat University Of Singapore And Agency For Science Technology And Res | Vascularized tissue, skin or mucosa quivalent |
| EP3643316A4 (en) * | 2017-06-19 | 2021-01-20 | National University Corporation Hokkaido University | Treatment agent for epidermolysis bullosa |
| CN111330066B (en) * | 2020-04-30 | 2022-05-20 | 西安交通大学医学院第一附属医院 | Three-dimensional structured biological dressing for repairing skin lesion of severe patient |
| CN117187174B (en) * | 2023-11-08 | 2024-02-06 | 广州正源生物技术有限公司 | Muse cell culture medium and extraction method of fat Muse cells |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120120971A (en) * | 2010-02-23 | 2012-11-02 | 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) | Methods for preparing human melanocytes from human pluripotent stem cells |
-
2014
- 2014-04-01 JP JP2015548081A patent/JP2016516394A/en active Pending
- 2014-04-01 WO PCT/JP2014/060045 patent/WO2014163206A1/en active Application Filing
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