JP2016079122A - Method for increasing amount of collagen in outer corner of eye and method for reducing conspicuous pores in cheek - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for increasing the amount of collagen in the outer corner of the eye and a method for reducing conspicuous pores in the cheek by oral ingestion of collagen peptide.SOLUTION: The invention provides a method for increasing the amount of collagen in the outer corner of the eye and a method for reducing conspicuous pores in the cheek by oral ingestion of collagen peptide and ornithine at least one time during three hours before falling asleep (but excluding medical practice in any method).SELECTED DRAWING: None

Description

  The present invention relates to a method for increasing the amount of collagen in the corner of the eye and a method for suppressing the conspicuousness of cheek pores.

  Human skin is composed of an outer layer, an epidermis, and a dermis from the outside. The dermis contains collagen, and more than half of the dermis is occupied by collagen. It is known that the amount of collagen contained in the dermis decreases with age, and the decrease in the amount of collagen in the dermis causes apparent aging such as wrinkles and sagging skin. Skin sagging can also cause pores to stand out. Therefore, it has been desired to develop a method for increasing the amount of collagen in the dermis.

  In recent years, many attempts have been made to incorporate collagen into cosmetics or foods and drinks into the body. For example, foods and beverages such as beverages containing collagen extracted from animals, fish and the like are widely supported by women who expect their skin-beautifying effects. Examples of foods and beverages containing collagen include collagen peptides having an average molecular weight of 1200 or less, beverages containing specific amino acids, ascorbic acid, etc. (see, for example, Patent Document 1), collagen, plant polyphenols, basic amino acids, etc. Food / beverage products to be performed (for example, refer to Patent Document 2) have been reported.

JP 2011-125329 A JP 2008-0795565 A

  By the way, since collagen taken orally is decomposed into peptides or amino acids in the body and absorbed and then reconstituted, reconstitution into collagen is efficiently performed to increase the amount of collagen in the body. It is necessary to be Moreover, even if the amount of collagen in the body increases, it is not always exhibited at a site where the effect of collagen is expected. Therefore, it is desired to develop a method for exerting the effect of collagen taken orally at a site where the effect is expected.

  This invention is made | formed in view of the said situation, and makes it a subject to provide the method of increasing the collagen amount of an eye corner, and the method of suppressing the conspicuousness of a cheek pore by ingesting a collagen peptide orally. .

  Specific means for solving the above problems are as follows.

[1] A method of increasing the amount of collagen in the corner of the eye by orally ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep (however, excluding medical practice).
[2] A method of suppressing the conspicuous pores in the cheeks by orally ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep (however, excluding medical practice).
[3] The method according to [1] or [2], wherein the intake amount of collagen peptide per time is 10,000 mg or more, and the intake amount of ornithine per time is 400 mg or more.
[4] The method according to any one of [1] to [3], wherein the collagen peptide and ornithine are ingested in a liquid, powder, or jelly form.

  According to the present invention, it is possible to provide a method for increasing the amount of collagen in the outer corner of the eye and a method for suppressing the conspicuousness of the pores of the cheeks by orally ingesting the collagen peptide.

It is a graph which shows the change of the number of pores of the cheek before and after continuous intake of the test food for 2 weeks. It is a graph which shows the change of the collagen score of the corner of the eye before and after continuous intake of the test food for 2 weeks.

  Hereinafter, specific embodiments of the present invention will be described in detail. However, the present invention is not limited to the following embodiments, and may be implemented with appropriate modifications within the scope of the object of the present invention. be able to.

  In the present specification, a numerical range indicated using “to” means a range including the numerical values described before and after “to” as the minimum value and the maximum value, respectively.

The method of the present invention is a method of increasing the amount of collagen in the corner of the eye by orally ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep (hereinafter referred to as “first method” as appropriate). .)
In the first method, the collagen peptide and ornithine are ingested in a specific manner, that is, by oral ingestion at least once during the period from 3 hours before falling asleep until falling asleep. Increases the amount of collagen specifically in the corner of the eye.

In addition, the method of the present invention is a method for suppressing the conspicuous pores on the cheeks by orally ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep. This method is referred to as “the method of the above”.
In the second method, the collagen peptide and ornithine are ingested in a specific manner, that is, by oral ingestion at least once during the period from 3 hours before falling asleep to falling asleep, compared to before ingestion, Suppresses the appearance of cheek pores.

Foods and drinks in which collagen peptide and ornithine are blended are known in the art, and collagen peptides and ornithine are blended in the foods and drinks described in Patent Document 1 and Patent Document 2 described above.
However, combining collagen peptide with ornithine and orally ingesting in a specific manner can provide an effect of specifically increasing the amount of collagen at the corner of the eye or making cheek pores inconspicuous compared to before ingestion. Nothing is known about that.
The method of the present invention is a useful method capable of exerting the effect of collagen taken orally at a specific site where the effect is expected.

  Hereinafter, in describing the method of the present invention (first method and second method), first, the collagen peptide and ornithine used in the method of the present invention will be described.

[Collagen peptide]
The collagen peptide used in the method of the present invention is not particularly limited.
Collagen peptide is a protein containing a large amount of glycine obtained by hydrolyzing gelatin extracted from collagen by heating with an enzyme, acid or the like, and is also available as a commercial product.
The collagen may be collagen extracted from mammalian collagen tissue, or collagen extracted from fish collagen tissue, and the origin of the collagen is not particularly limited. Examples of mammals include pigs and cows. Moreover, as fish, saltwater fish may be sufficient and freshwater fish may be sufficient, for example, a tuna (yellowfin), shark, cod, flounder, flounder, Thailand, tilapia, salmon, catfish, etc. are mentioned.
From the viewpoint of easy availability and low cost, the collagen is preferably fish-derived collagen. Fish-derived collagen is collagen extracted from fish scales, skins, and the like.

The weight average molecular weight of the collagen peptide is preferably 1000 to 3000, more preferably 1500 to 2700, and more preferably 1800 to 2500 from the viewpoint of ease of application to a liquid or jelly form. Further preferred.
The weight average molecular weight of the collagen peptide can be confirmed by gel permeation chromatography (GPC: polyethylene glycol (PEG) standard). When using a commercial product, product information provided by the supplier may be followed.
In the method of the present invention, one type of collagen peptide may be used alone, or two or more types of collagen peptides may be used in combination.

[Ornithine]
Ornithine used in the method of the present invention is not particularly limited.
Ornithine is a kind of amino acid and can contribute to the improvement of the production efficiency and utilization efficiency of collagen in the body.
Ornithine may be any of L-form, D-form, and DL-form, and L-form is preferred from the viewpoint of availability.
As ornithine, those obtained by a chemical synthesis method, a fermentation production method, or the like may be used, or a commercially available product may be used.
Ornithine may be in the form of inorganic acid salts such as hydrochloride, phosphate, acetate, fumarate, maleate, citrate, malate, α-ketoglutarate, aspartate It may be in the form of an organic acid salt.
In the method of the present invention, one kind of ornithine may be used alone, or two or more kinds of ornithine may be used in combination.

  Hereinafter, the method of the present invention will be described in order by dividing it into items common to the first method, the second method, and the first method and the second method.

[First method]
The first method is a method of increasing the amount of collagen in the corner of the eye by orally ingesting the collagen peptide and ornithine at least once between 3 hours before falling asleep and falling asleep.
In the first method, the collagen peptide and ornithine are ingested in a specific manner that they are taken orally at least once from 3 hours before falling asleep until falling asleep. Increase the amount of collagen.

The “eye corner” in the present invention refers to an end portion on the ear side of the eye and its peripheral region.
The method for measuring the “collagen amount” in the present invention is not particularly limited as long as it can directly or indirectly measure the amount of collagen. As a method that can be indirectly measured, a measurement method in which a specific physical property value in the skin has a correlation with the amount of collagen in the skin can be employed. For example, the amount of collagen can be objectively quantified from a collagen score measured using an ultrasonic dermis image device (device name: DermaLab (registered trademark), model number: DermaLab) manufactured by Cortex Technology.

[Second method]
The second method is a method of suppressing the conspicuousness of cheek pores by orally ingesting a collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep.
In the second method, collagen peptide and ornithine are ingested in a specific manner of ingestion at least once during the period from 3 hours before sleep onset to sleep onset, so that the pores on the cheeks are compared with those before intake. Suppresses the noticeability.

The “conspicuous pores” in the present invention means that the area of each pore becomes large and the pores are conspicuous, and does not include conspicuous dark pores due to clogging of the pores.
The suppression of “conspicuous pores” in the present invention should be confirmed by measuring the number of pores, the size of pores, etc. from the image of the surface of the skin (skin) and reducing the number of pores, the size of pores, etc. Can do. For example, suppression of pore conspicuousness should be confirmed by a decrease in the number of pores measured using a skin image analyzer (device name: VISIA (registered trademark) Evolution, model number: VISIA-Evo) manufactured by Canfield Scientific. Can do. It means that the pore whose number was measured by the above apparatus was recognized as a conspicuous pore.

[Matters common to the first method and the second method]
In the method of the present invention (the first method and the second method), between 3 hours before falling asleep and falling asleep, preferably 2 hours before falling asleep until falling asleep, more preferably 1 hour after falling asleep. Collagen peptide and ornithine are taken orally between before and after falling asleep, more preferably just before falling asleep.
It is presumed that collagen peptides taken orally are absorbed as they are, or decomposed into amino acids in the body and absorbed and then reconstituted into collagen. In the method of the present invention, the collagen peptide and ornithine are orally ingested between 3 hours before falling asleep and falling asleep, so that the collagen peptide is decomposed and absorbed in the body, collagen is produced by ornithine, and collagen is produced (re-generated). The composition is considered to be performed efficiently at the timing of about 1 hour after falling asleep when growth hormone secretion peaks. Such a phenomenon is presumed to be one factor that leads to the realization of the effect expected of a collagen peptide at a specific site, such as an effect of increasing the amount of collagen in the corner of the eye or an effect of suppressing the conspicuousness of cheek pores. The The above estimation is not intended to limit the effect of the present invention, but is described as an example.

The number of oral intakes of the collagen peptide and ornithine between 3 hours before falling asleep and falling asleep may be one or more, and may be multiple.
The number of times can be appropriately set according to the type of collagen peptide, the intake amount of collagen peptide and ornithine per dose, and the like. The number here means the number of times of ingestion of the combination of collagen peptide and ornithine, for example, when collagen peptide and ornithine are taken separately and sequentially, instead of taking collagen peptide and ornithine at once. Counts the combined intake of collagen peptide and ornithine as one time.

In the method of the present invention, the collagen peptide and ornithine may be ingested in a lump or may be ingested separately and sequentially. From the viewpoint of increasing the amount of collagen in the outer corner of the eye or suppressing the conspicuousness of cheek pores, it is preferable to ingest the collagen peptide and ornithine in a lump, and the collagen peptide and ornithine are ingested separately and sequentially. In some cases, it is preferable that the time difference between the intake time of collagen and the intake time of ornithine is as small as possible.
The time difference between the intake time of collagen and the intake time of ornithine is not particularly limited. The time difference between the collagen intake time and the ornithine intake time is preferably within 2 hours, more preferably within 1 hour, further preferably within 30 minutes, and particularly preferably within 10 minutes.
As a method of ingesting collagen peptide and ornithine at once, a method of ingesting as a composition containing collagen peptide and ornithine, a collagen peptide alone or a composition containing it, and an ornithine alone or a composition containing it are separated. In the mouth, swallowed together and ingested.

In the method of the present invention, the intake amount of collagen peptide and ornithine per time is particularly limited as long as it is an amount that can increase the amount of collagen in the corner of the eye or suppress the conspicuous pores of the cheeks. It is not a thing.
From the viewpoint of further enhancing the effects of collagen peptide and ornithine, it is preferable that the intake amount of collagen peptide per time is 3000 mg or more, and the intake amount of ornithine per time is preferably 80 mg or more. More preferably, the intake per time is 5000 mg or more, and the intake per ornithine is more than 250 mg, the intake per collagen peptide is more than 10,000 mg, and ornithine It is more preferable that the intake per time is 400 mg or more.
The upper limit value of the intake amount of collagen peptide and ornithine per time is not particularly limited. In general, the upper limit of the intake amount of collagen peptide is preferably 30000 mg or less, and the upper limit of the intake amount of ornithine is preferably 1200 mg or less.

  If the total intake amount of collagen peptide and ornithine taken orally between 3 hours before sleep onset and sleep on is the same, the intake method with fewer intakes and higher intake per time is collagen From the viewpoint of further enhancing the effect of the peptide and ornithine, it is preferable.

  The ratio of collagen peptide intake and ornithine intake (collagen peptide intake: ornithine intake) is from the viewpoint of increasing the collagen content of the corner of the eye, or from the viewpoint of further suppressing the conspicuousness of the cheek pores, On a mass basis, it is preferably 1000: 1 to 1: 1, more preferably 500: 1 to 5: 1, and even more preferably 100: 1 to 10: 1.

In the method of the present invention, from the viewpoint of maintaining the effect of increasing the amount of collagen in the outer corner of the eye or the effect of suppressing the conspicuousness of the pores in the cheeks, the collagen peptide and ornithine are orally ingested at least once from 3 hours before falling asleep until falling asleep. This is preferably done continuously, more preferably every day.
The number of days to be continued is not particularly limited as long as the effect of increasing the amount of collagen in the outer corner of the eye or the effect of suppressing the conspicuousness of the cheek pores is exhibited.
For example, when 10000 mg of collagen peptide and 400 mg of ornithine are orally ingested once a day, the continuous days more reliably express the effect of increasing the amount of collagen in the corner of the eye or suppressing the conspicuousness of pores in the cheeks. It is preferable that it is 14 days or more from a viewpoint made to do.

In the method of the present invention, the intake form of the collagen peptide and ornithine is not particularly limited. From the viewpoint of ease of ingestion, it is preferable to orally ingest each of the collagen peptide and ornithine in a liquid, powder, or jelly form.
The intake form of the collagen peptide and ornithine may be the same or different. From the viewpoint of ease of ingestion, collagen peptide and ornithine are preferably ingested in the same form, and more orally ingested as a liquid composition, powder composition, or jelly composition containing collagen peptide and ornithine. preferable.
Examples of the liquid composition containing collagen peptide and ornithine include beverages such as soft drinks, alcoholic beverages and soups containing collagen peptide and ornithine.
A jelly composition containing a collagen peptide and ornithine can be prepared, for example, by adding a gelling agent to the collagen peptide and ornithine.

When the collagen peptide and ornithine are ingested as a composition containing the collagen peptide and ornithine, other components can be blended in the composition as necessary within a range not impairing the effects of the present invention.
Examples of other components include components that are generally accepted for use in food and drink. Such other components include, for example, sweeteners such as high-intensity sweeteners, sugar alcohols, and sucrose; phosphoric acid, citric acid, tartaric acid, phytic acid, malic acid, ascorbic acid, lactic acid, succinic acid, fumar Acids and other acidulants; amino acids such as lysine and proline (excluding ornithine); gelatinizers such as gelatin, agar and carrageenan (excluding collagen); minerals; various vitamins; coloring agents; antioxidants; Emulsifier; antifoaming agent; thickener; fragrance and the like.

When ingesting collagen peptide and ornithine in the form of powder, it is preferable to ingest with sufficient water. Examples of water include beverages such as soft drinks and soups that do not contain components that may cause undesirable interactions in combination with collagen peptides and ornithine in addition to water.
In addition, collagen peptide and ornithine in powder form may be dissolved or dispersed in the beverage, and a liquid composition containing collagen peptide and ornithine may be prepared at the time of use and ingested.

  The method of the present invention is not intended to treat or diagnose humans, and the method of the present invention excludes medical practice.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to the following examples unless it exceeds the gist thereof.

[Preparation of test food]
Collagen peptide (fish-derived, average molecular weight: 2000 to 3000, Kyowa Hakko Bio) powder and ornithine powder (trade name: L-ornithine hydrochloride Kyowa, L-ornithine hydrochloride, Kyowa Hakko Bio) The mixture was thoroughly mixed at a mass ratio [collagen peptide: ornithine (ornithine converted value)] of 25: 1. The obtained mixture was divided into 10400 mg portions to prepare a test meal.

[Study food intake]
Fourteen test meals were provided to 13 female subjects in their 30s and 40s. Each subject took the test meal dissolved in hot water with a commercially available powdered soup (37.7 kJ / 1 meal) once a day, from 3 hours before falling asleep until falling asleep, and this was continued for 2 weeks. .

[Measurement of skin condition]
Skin image analysis device (device name: VISIA (registered trademark) Evolution, model number: VISIA-Evo, manufactured by Canfield Scientific, analysis item: stain, wrinkle, and so on) Number of pores), skin viscoelasticity measuring device (device name: Cutometer (registered trademark) DUAL MPA580, model number: MPA580Dual, manufactured by Courage + Khazaka, measurement item: skin viscoelasticity), and ultrasonic dermis imaging device (device name: Measurement was performed using DermaLab (registered trademark), model number: DermaLab, manufactured by Cortex Technology, measurement item: collagen score.
The number of spots, wrinkles, and pores after 2 weeks of continuous intake of the test food was calculated as a relative value when the number of spots, wrinkles, and pores before 2 weeks of continuous intake of the test food was 100%. And compared. The skin viscoelasticity and collagen score were compared before and after continuous intake of the test food for 2 weeks.

The results of the number of cheek pores and the amount of collagen in the corners of the eye where significant effects were recognized are shown in FIGS. 1 and 2, respectively.
The measurement results shown in FIG. 1 and FIG. 2 are expressed as mean ± standard deviation (SD). Statistical analysis was performed with Microsoft (registered trademark) Excel (statistical analysis software: Excel statistics), and the significance level was set to 5% by two-sided test.
Table 1 shows details of the measuring device, measurement items, and measurement site.

As a result of the above measurement, as shown in FIG. 1, a significant decrease in the number of pores on the cheek of the test subject was observed before and after continuous intake of the test food for 2 weeks.
In addition, as shown in FIG. 2, a significant increase was observed in the collagen score of the eye corners of the subject before and after continuous intake of the test food for 2 weeks.

  On the other hand, no significant difference was observed between the number of cheek spots and wrinkles, the skin viscoelasticity of the cheek, mouth, corner of the eyes, and neck muscles, and the collagen score of the cheek before and after the continuous intake of the test food for 2 weeks.

  From the above results, it is clear that oral intake of collagen peptide and ornithine from 3 hours before falling asleep until falling asleep reduces the number of pores on the cheeks and increases the amount of collagen in the corner of the eyes. became.

Claims (4)

  A method of increasing the amount of collagen in the corner of the eye by orally ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep (however, excluding medical practice).   A method of suppressing the conspicuous pores in the cheeks by ingesting collagen peptide and ornithine at least once during the period from 3 hours before falling asleep until falling asleep (however, excluding medical practice).   The method according to claim 1 or 2, wherein the intake amount of collagen peptide per time is 10,000 mg or more, and the intake amount of ornithine per time is 400 mg or more.   The method according to any one of claims 1 to 3, wherein the collagen peptide and ornithine are ingested in a liquid, powder, or jelly form.