JP2016050173A - Medicine for intestinal disorders containing resistant glucan, and production method thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel medicine for intestinal disorders for the improvement of constipation and intestinal environment.SOLUTION: The invention provides a resistant glucan consisting of sugar condensate obtained by heating and condensing starch degradation products of DE 70-100 to under the presence of activated carbon, at 100-300°C; or a medicine for intestinal disorders containing the resistant glucan-processed product. Preferably, the heat process is carried out so that the dietary fiber content of resistant glucan (sugar condensate) may be 70% or more, for example, at 100-300°C for 1-180 minutes, more preferably, at 150-250°C for 1-180 minutes.SELECTED DRAWING: None

Description

  The present invention relates to an intestinal regulating agent containing an indigestible glucan as an active ingredient and a method for producing the same.

  The number of people suffering from constipation has been increasing in recent years due to disordered eating habits, westernization of eating habits, and increased stress. Constipation causes stool to stay for a long time in the intestine, causing an increase in harmful bacteria in the intestine or an increase in the absorption of harmful substances, which also causes colon cancer. That is, recent studies have shown that improvement of the intestinal environment is greatly related to human health maintenance and disease prevention. Therefore, it has been proposed to improve constipation and intestinal environment by ingesting foods and pharmaceuticals having an intestinal regulating action.

  To improve constipation, various compounds or materials having an effect of improving bowel movement have been proposed. For example, dietary fiber such as pectin, carrageenan, inulin, indigestible dextrin, wheat bran, xylooligosaccharide, fructo-oligo Oligosaccharides such as sugar, galactooligosaccharide and lactulose are known (Patent Documents 1 to 4). However, even with these substances, depending on the constitution, diarrhea or no effect occurs, and it cannot be said that the effect of improving bowel movement is sufficient. In addition, some substances may be obtained by extraction and purification from agricultural crops or require special enzymes for production, and thus have problems in terms of supply stability and production cost. Furthermore, the development of a new intestinal regulating agent has been desired also in the sense of expanding the options for the intestinal regulating agent.

Japanese Patent Publication No. 07-12294 JP 2002-51731 A JP 2003-12537 A JP 2009-167172 A

  An object of this invention is to provide a new intestinal regulating agent.

  The inventors of the present application have found that an indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 has an intestinal regulating action in vivo. The present invention is based on this finding.

That is, the present invention is as follows.
(1) An intestinal adjuster comprising an indigestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a processed product of the indigestible glucan.
(2) The intestinal regulating agent according to (1), wherein the indigestible glucan comprises a sugar condensate obtained by heat condensation in the presence of activated carbon.
(3) The intestinal regulating agent according to (1) or (2), wherein the indigestible glucan comprises a sugar condensate obtained by heat condensation at 100 to 300 ° C.
(4) The indigestible glucan consisting of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a processed product of the indigestible glucan is added, (1) to (3 ). The method for producing an intestinal regulating agent according to any one of the above.
(5) The indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink comprising the indigestible glucan-treated product is ingested, Intestinal adjustment method (excluding medical practice).
(6) The indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink containing the indigestible glucan-treated product is ingested, How to improve bowel movement (excluding medical practice).

  According to the present invention, the use of indigestible glucan is advantageous in that a novel intestinal regulating agent can be provided.

It is the figure which showed the design of the test. It is the figure which showed the variation | change_quantity (average value +/- standard deviation) of the "defecation days" of a period I and a period II. It is the figure which showed variation | change_quantity (average value +/- standard deviation) of "the number of defecations" of a period I and a period II. It is the figure which showed the variation | change_quantity (average value +/- standard deviation) of the "defecation amount" of a period I and a period II.

Detailed description of the invention

  The intestinal regulating agent according to the present invention is an intestinal regulating agent comprising an indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a processed product of the indigestible glucan.

  In the present invention, “indigestible glucan” means an indigestible glucan (glucose polymer), and can be obtained as a sugar condensate obtained by subjecting a starch degradation product of DE 70 to 100 to a condensation reaction by heat treatment. it can. Indigestible glucan is rich in water-soluble dietary fiber fraction.

  As a starch degradation product that is a raw material for the indigestible glucan, a starch degradation product having a DE of 70 to 100 can be used. When the DE of the starch degradation product is less than 70, the decomposition is insufficient, and thus the structure derived from starch remains in the indigestible glucan obtained and tends to be easily decomposed by the enzyme in the body and easily absorbed. And is not preferable in terms of the effect of regulating the intestines. Here, “DE (Dextrose Equivalent)” is an index of the degree of degradation of the starch degradation product, and is a value expressed as a percentage of the solid content with the reducing sugar in the sample as glucose. The starch degradation product preferably has a DE of 75 to 100, more preferably 80 to 100. The “degraded starch of DE 70 to 100” used in the present invention may be any starch degraded product in which DE satisfies a predetermined range, and examples thereof include maltooligosaccharides, starch syrup, powder cake, glucose and the like. There are no particular restrictions on the properties, and any of crystalline products (anhydrous glucose crystals, hydrous glucose crystals, etc.), liquid products (liquid glucose, starch syrup, etc.), and non-crystalline powder products (powder etc.) can be used. In view of the above, it is preferable to use a liquid product. In particular, the use of glucose syrup called “hydrol” which is a by-product generated in the purification process of glucose is extremely advantageous from the viewpoints of recycling and raw material cost reduction.

  In the present invention, “heat condensation” refers to condensation of a starch decomposition product under heating conditions, and the heat condensation method is well known to those skilled in the art. The heating conditions in the heat condensation are not particularly limited as long as an indigestible glucan (sugar condensate) rich in water-soluble dietary fiber is obtained by the condensation reaction, and those skilled in the art can appropriately determine the heating conditions. It is preferable to heat the resulting indigestible glucan (sugar condensate) so that the dietary fiber content is 70% or more, for example, 100 ° C. to 300 ° C. for 1 to 180 minutes, more preferably 150 ° C. to Heat treatment can be performed at 250 ° C. for 1 to 180 minutes.

  In the present invention, “heat condensation” may be performed under non-catalytic conditions, but is preferably performed in the presence of a catalyst from the viewpoint of the reaction efficiency of the condensation reaction. The catalyst is not particularly limited as long as it catalyzes a sugar condensation reaction, but inorganic acids (hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, etc.), organic acids (citric acid, fumaric acid, tartaric acid, succinic acid, acetic acid, etc.) ), Mineral substances (diatomaceous earth, activated clay, acidic clay, bentonite, kaolinite, talc, etc.) and activated carbon (steam coal, zinc chloride charcoal, sulfonated activated carbon, oxidized activated carbon) can be used. In consideration of coloring and safety of the resulting water-soluble dietary fiber material, as well as taste and odor, it is preferable to use activated carbon as a catalyst. Moreover, each said catalyst can also be used in combination of 2 or more type.

  As the indigestible glucan used in the present invention, the sugar condensate obtained by the above method may be used as it is, or various processed products of indigestible glucan may be used. Examples of the indigestible glucan-treated product include an indigestible glucan enzyme-treated product, an indigestible glucan fraction-treated product, and an indigestible glucan reduction-treated product.

  In the present invention, the “indigestible glucan enzyme-treated product” can be obtained by enzymatic treatment of a sugar condensate with a saccharide-degrading enzyme. Since the digestible site | part in indigestible glucan can be decomposed | disassembled by the said process, a dietary fiber content can be raised.

  The “carbohydrase” used in the present invention is an enzyme that acts on a carbohydrate and catalyzes a hydrolysis reaction, and is not particularly limited. For example, α-amylase, β-amylase, glucoamylase (amyloglucosidase) is used. , Isoamylase, pullulanase, α-glucosidase, cyclodextrin glucanotransferase, β-glucosidase, β-galactosidase, β-mannosidase, β-fructosidase, cellobiase, gentiobiase, etc. Alternatively, a plurality of enzymes may be used in combination. Α-Amylase and glucoamylase are preferred from the action of degrading into indigestible glucan, and either of these enzymes may be allowed to act alone, but it is particularly preferred that α-amylase and glucoamylase are allowed to act together.

  In the present invention, the treatment conditions for the “enzyme treatment” are not particularly limited as long as the digestible portion of the sugar condensate is digested by the enzyme treatment, and those skilled in the art can appropriately determine the enzyme treatment conditions. However, it is preferable that the glucose content is increased by 1% or more, more preferably 2% or more by enzyme treatment, for example, 20 to 120 ° C. for 30 minutes to 48 hours, more preferably 50 to 100 ° C. For 30 minutes to 48 hours.

  In the present invention, the “indigestible glucan fraction-treated product” can be obtained by fractionating the indigestible glucan enzyme-treated product so that the fraction of disaccharide or less is 15% or less. In other words, the “digestible glucan fraction-treated product” has more than 85% of saccharides of three or more sugars. Here, “%” means mass% with respect to the solid content.

  In the present invention, the “fractionation treatment” is not particularly limited as long as the fraction less than or equal to the disaccharide can be reduced to 15% or less, and the separation method can use means well known to those skilled in the art. .

  The fractionation treatment uses, for example, carbohydrate purification methods well known to those skilled in the art, such as membrane separation, gel filtration chromatography, carbon-celite column chromatography, strong acid cation exchange column chromatography, ethanol precipitation, and solvent precipitation. can do.

  The fractionation treatment is preferably performed so that the fraction of disaccharides or less is 10% or less, and particularly preferably 5% or less.

  In the present invention, the “reduced product of indigestible glucan” can be obtained by reducing the indigestible glucan.

  In the present invention, “reduction treatment” refers to treatment for reducing the aldehyde group of the glucosyl group at the reducing end of a sugar to a hydroxyl group. The reduction treatment method is well known to those skilled in the art, and examples thereof include a method using a hydride reducing agent, a method using a metal in a protic solvent, an electrolytic reduction method, and a catalytic hydrogenation reaction method. In the present invention, when preparing a small amount of sugar alcohol, the method using a hydride reducing agent is convenient and convenient without requiring a special device, while on the other hand, when carried out industrially on a large scale, A method using a catalytic hydrogenation reaction is preferable because it is economical and has few by-products. The “catalytic hydrogenation reaction” is a reaction in which hydrogen is added to a double bond portion of an unsaturated organic compound in the presence of a catalyst, and is generally also referred to as a hydrogenation reaction.

  The “reduction treatment” in the present invention will be specifically described. Indigestible glucan is dissolved in water, an appropriate amount of Raney nickel catalyst is added thereto, hydrogen gas is added, and reduction is performed under high temperature conditions. Next, decolorization and deionization treatment can be performed to obtain an indigestible glucan reduction treatment product.

  In the present invention, the “enteric agent” means a preparation that improves the intestinal environment and improves symptoms such as diarrhea and constipation. The intestinal regulating agent according to the present invention is particularly a bowel movement improving agent.

  The intestinal preparation according to the present invention only needs to contain indigestible glucan, and may be indigestible glucan alone or mixed with other components.

  The intestinal preparation according to the present invention can be formulated by adding a pharmaceutically acceptable base or carrier to the indigestible glucan, which is an active ingredient, as necessary. The intestinal preparation according to the present invention is, for example, a preparation that is administered orally, and can be provided in the form of tablets, granules, powders, solutions, powders, granules, capsules and the like. Such a formulation can be usually produced according to a method used for producing a medicine. According to the present invention, the intestinal preparation according to the present invention can be used as a medicine or a quasi drug.

  The intestinal regulating agent of the present invention can also be provided by being added to food and drink. There are no particular restrictions on the types of foods and drinks to be added, such as foods and drinks to be eaten on a daily basis, health foods (food for specified health use, nutritional functional foods, nutritional supplements, etc.), functional foods, foods for the sick, etc. Can be provided. Examples of the form include foods and drinks, tablets, liquids, capsules (soft capsules, hard capsules), powders, granules, sticks, jelly, and the like. Such foods and drinks can usually be produced according to the methods used for producing foods. The “special health food” refers to a food that may be subject to some legal restrictions from the viewpoint of health when the function or the like is displayed to manufacture or sell the food.

  The amount of the intestinal adjuster according to the present invention is not particularly limited and can be set as appropriate. For example, the indigestible glucan or the processed product thereof can be set so that 1 to 30 g, preferably 3 to 10 g, is ingested as the amount of dietary fiber per day.

    According to the present invention, an indigestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70-100 or a composition for intestinal regulation comprising the indigestible glucan treated product, particularly Provides a composition for improving bowel movements.

  According to the present invention, an indigestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a treatment product of the indigestible glucan is added. A method is provided.

  According to the present invention, indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink comprising the indigestible glucan-treated product is ingested. And an intestinal regulation method is provided. The method may exclude medical practice.

  According to the present invention, indigestible glucan composed of a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink comprising the indigestible glucan-treated product is ingested. A method for improving bowel movement is provided. The method may exclude medical practice.

  ADVANTAGE OF THE INVENTION According to this invention, the intestinal adjustment method using the indigestible glucan which consists of a sugar condensate obtained by heat condensing the starch degradation product of DE70-100, or this indigestible glucan processed material is provided. The method may exclude medical practice.

  ADVANTAGE OF THE INVENTION According to this invention, the bowel movement improvement method using the indigestible glucan which consists of a sugar condensate obtained by heat condensing the starch degradation product of DE70-100, or this indigestible glucan processed product is provided. The method may exclude medical practice.

  The present invention will be specifically described based on the following examples, but the present invention is not limited to these examples.

Example: Confirmation test of intestinal action of indigestible glucan
Table 1 shows the formulation of the test food test food.

  As the indigestible glucan, Fit Fiber # 80 (manufactured by Nippon Shokuhin Kako) was used. Fit fiber # 80 is a sugar condensate obtained by subjecting a starch degradation product of DE87 (measured by the Lane Einon method) to heat condensation using activated carbon as a catalyst according to the method described in JP2013-76044A.

  As the maltodextrin, Paindex # 2 (manufactured by Matsutani Chemical Industry Co., Ltd.) was used. In addition, the color tone of maltodextrin was adjusted using a coloring agent (caramel) so that it might become the same color as indigestible glucan.

A schematic design tests of the test in Table 2 showed a study design in Figure 1.

  The test food was taken once a day during the intake period I and intake period II (4 weeks in total) and dissolved in about 100 mL of water or hot water in about 5 minutes on an empty stomach before breakfast. . If there was a precipitate after ingestion, water was poured again to ingest everything.

Examination and investigation subjects are the test period (pre-observation period, ingestion period I, ingestion period II: total 6 weeks), daily intake of test food (ingestion period only), defecation time, number of defecations, defecation volume In addition, the time of defecation, the presence or absence of the use of pharmaceuticals including intestinal and constipation drugs, the presence or absence of physiology, the presence or absence of changes in physical condition mainly in the gastrointestinal symptoms, and the presence or absence of changes in lifestyle were recorded.

Evaluation items and evaluation criteria were as follows.
Defecation days: Count the number of days of defecation per week.
Number of defecation: Investigate the number of defecations per day. (If there is no defecation, the number of defecation is set to "0")
Defecation amount: Using M1 chicken eggs as a guide, numerical value is obtained by subject's visual observation. (If it is not divisible by one egg, fill in 0.5 and 1.5)

  Subjects are also allowed to eat meals, snacks, and prohibited foods (foods containing live bacteria such as lactic acid bacteria, bifidobacteria, and natto) during the study period (pre-observation period, intake period I, intake period II: 6 weeks total). , Oligosaccharides and dietary fiber reinforced foods, health foods (including foods for specified health use) that are considered good for constipation, and sugar alcohol-rich foods. All recorded. Nutrition calculations are not performed.

Analysis of effectiveness Regarding the number of defecation days, the number of defecations, and the amount of defecation, the following procedures were compared in the previous observation period, stage I, and stage II.
1) Calculate basic statistics (average value, standard deviation).
2) A basic statistic (average value, standard deviation) is calculated for the amount of change (Δ) between stage I and stage II.
3) Intragroup comparison: Wilcoxon signed rank test and paired t test are performed for stage I and stage II. (Phase I vs Phase II)
4) Comparison between groups: Steel test and Dunnett test are performed for the pre-observation period, stage I, and stage II. (Control Food Group vs Low Dose Group, Control Food Group vs Medium Dose Group, Control Food Group vs High Dose Group)

Test results The number of defecation days, the number of defecations and the amount of defecation were analyzed, and the amount of change in stage I and stage II was shown in FIGS. 2, 3 and 4, respectively.

  The number of days of defecation was significantly higher in stage II than in stage I (control food) in the high-dose group (paired t-test: p = 0.011, Wilcoxon signed rank test: p = 0.012 ). In addition, regarding the amount of change between stage I and stage II, the medium dose group and the high dose group showed a dose-dependent increasing tendency compared to the control food group (FIG. 2).

  The number of defecations was significantly higher in stage II compared to stage I (control food) in the high dose group (paired t test: p = 0.024, Wilcoxon signed rank test: p = 0.026 ). In addition, regarding the amount of change between stage I and stage II, the low-dose group, the middle-dose group, and the high-dose group showed a dose-dependent increase tendency compared to the control food group (FIG. 3).

  Regarding defecation, the high dose group showed significantly higher values in stage II compared to stage I (control food) (paired t test: p = 0.026, Wilcoxon signed rank test: p = 0.04968) ). In addition, regarding the amount of change between stage I and stage II, the low-dose group, medium-dose group, and high-dose group showed an increasing tendency as compared to the control food group (FIG. 4).

  By this test, the indigestible glucan according to the present invention showed significantly higher values in the stage II compared to the stage I (control food) in terms of the number of defecation days, the number of defecations, and the amount of defecation. Compared to the control food group, and the number of defecations was increased in a dose-dependent manner compared to the control food group (low dose group, medium dose group, High dose group), and the amount of defecation showed an increasing tendency compared to the control food group (low dose group, medium dose group, high dose group). It is confirmed from the subject's records that other conditions do not affect the determination of effectiveness.

  From the above, it was shown that the indigestible glucan according to the present invention has an intestinal regulating action and is useful as an intestinal regulating agent.

Claims (6)

An intestinal regulating agent comprising an indigestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a processed product of the indigestible glucan.   The intestinal regulating agent according to claim 1, wherein the hardly digestible glucan comprises a sugar condensate obtained by heat condensation in the presence of activated carbon.   The intestinal regulating agent according to claim 1 or 2, wherein the indigestible glucan comprises a sugar condensate obtained by heat condensation at 100 to 300 ° C.   The indigestible glucan consisting of a sugar condensate obtained by heat-condensing a starch degradation product of DE 70 to 100 or a processed product of the indigestible glucan is added. The manufacturing method of an intestinal regulating agent of claim | item.   A method for regulating intestines, comprising ingesting an indigestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink comprising the indigestible glucan-treated product (Excluding medical practice).   A method for improving bowel movements, comprising ingesting a non-digestible glucan comprising a sugar condensate obtained by heat condensation of a starch degradation product of DE 70 to 100 or a food or drink comprising the non-digestible glucan-treated product (Excluding medical practice).

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