JP2016014025A5 - - Google Patents

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JP2016014025A5
JP2016014025A5 JP2015141987A JP2015141987A JP2016014025A5 JP 2016014025 A5 JP2016014025 A5 JP 2016014025A5 JP 2015141987 A JP2015141987 A JP 2015141987A JP 2015141987 A JP2015141987 A JP 2015141987A JP 2016014025 A5 JP2016014025 A5 JP 2016014025A5
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hydrogel
conjugate
solution
flavonoid
forming substance
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JP2015141987A
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Japanese (ja)
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JP2016014025A (en
JP6112576B2 (en
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Claims (17)

ハイドロゲル形成物質とフラボノイドとのコンジュゲートを含むハイドロゲルを生成するための方法であって、外から加える過酸化物の非存在下およびペルオキシダーゼの非存在下で、溶液中で該コンジュゲートを化合させる段階を含む、方法。   A method for producing a hydrogel comprising a conjugate of a hydrogel-forming substance and a flavonoid, wherein the conjugate is combined in solution in the absence of externally added peroxide and in the absence of peroxidase. A method comprising the steps of: 前記溶液のpHを改変することによって、前記ハイドロゲルのゲル化速度を制御する段階を含む、請求項1記載の方法。2. The method of claim 1, comprising controlling the gelation rate of the hydrogel by modifying the pH of the solution. pHが3から10の間で改変される、請求項1または2記載の方法。The method according to claim 1 or 2, wherein the pH is modified between 3 and 10. pHが6から8の間で改変される、請求項1〜3のいずれか一項記載の方法。The method according to any one of claims 1 to 3, wherein the pH is modified between 6 and 8. カタラーゼを前記溶液に加える段階を含む、請求項1〜4のいずれか一項記載の方法。5. A method according to any one of claims 1 to 4, comprising the step of adding catalase to the solution. ハイドロゲル形成物質とフラボノイドとのコンジュゲートを含むハイドロゲルを生成するための方法であって、
（i）外から加える過酸化物の非存在下で、溶液中で該コンジュゲートとペルオキシダーゼを化合させる段階、および
（ii）該溶液のpHを改変することによってハイドロゲルのゲル化速度を制御する段階 A method for producing a hydrogel comprising a conjugate of a hydrogel-forming substance and a flavonoid, comprising:
(I) combining the conjugate with peroxidase in solution in the absence of externally added peroxide; and (ii) controlling the gelation rate of the hydrogel by modifying the pH of the solution. Stage フラボノイドがカテキン骨格フラボノイドである、請求項1〜6のいずれか一項記載の方法。The method according to any one of claims 1 to 6, wherein the flavonoid is a catechin skeleton flavonoid. フラボノイドが没食子酸エピガロカテキンである、請求項1〜7のいずれか一項記載の方法。The method according to any one of claims 1 to 7, wherein the flavonoid is epigallocatechin gallate. ハイドロゲル形成物質がポリマーである、請求項1〜8のいずれか一項記載の方法。The method according to any one of claims 1 to 8, wherein the hydrogel-forming substance is a polymer. ハイドロゲル形成物質がヒアルロン酸である、請求項1〜9のいずれか一項記載の方法。The method according to any one of claims 1 to 9, wherein the hydrogel-forming substance is hyaluronic acid. 前記溶液中の前記コンジュゲートの濃度が、0.1 mg/ml〜500 mg/mlである、請求項1〜10のいずれか一項記載の方法。The method according to any one of claims 1 to 10, wherein the concentration of the conjugate in the solution is 0.1 mg / ml to 500 mg / ml. 前記溶液中の前記コンジュゲートの濃度が、1 mg/ml〜100 mg/mlである、請求項1〜11のいずれか一項記載の方法。12. The method according to any one of claims 1 to 11, wherein the concentration of the conjugate in the solution is 1 mg / ml to 100 mg / ml. 生物活性物質を前記溶液中で前記コンジュゲートと化合させる段階をさらに含む、請求項1〜12のいずれか一項記載の方法。13. The method according to any one of claims 1 to 12, further comprising combining a bioactive substance with the conjugate in the solution. 生物活性物質が抗癌剤である、請求項13記載の方法。14. The method according to claim 13, wherein the bioactive substance is an anticancer agent. 抗癌剤がハーセプチンである、請求項14記載の方法。15. The method according to claim 14, wherein the anticancer agent is Herceptin. 請求項1〜15のいずれか一項記載の方法によって生成された、ハイドロゲル形成物質とフラボノイドとのコンジュゲートを含むハイドロゲル。 A hydrogel comprising a conjugate of a hydrogel-forming substance and a flavonoid produced by the method of any one of claims 1-15 . 請求項16記載のハイドロゲルを細胞に接触させる段階を含む、フラボノイドを細胞に送達するための方法。 17. A method for delivering a flavonoid to a cell comprising the step of contacting the cell with a hydrogel according to claim 16 .
JP2015141987A 2009-05-29 2015-07-16 Flavonoid hydrogel Expired - Fee Related JP6112576B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21333109P 2009-05-29 2009-05-29
US61/213,331 2009-05-29

Related Parent Applications (1)

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JP2012513017A Division JP5843758B2 (en) 2009-05-29 2010-05-17 Flavonoid hydrogel

Publications (3)

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JP2016014025A JP2016014025A (en) 2016-01-28
JP2016014025A5 true JP2016014025A5 (en) 2016-03-10
JP6112576B2 JP6112576B2 (en) 2017-04-12

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JP2012513017A Expired - Fee Related JP5843758B2 (en) 2009-05-29 2010-05-17 Flavonoid hydrogel
JP2015141987A Expired - Fee Related JP6112576B2 (en) 2009-05-29 2015-07-16 Flavonoid hydrogel

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JP2012513017A Expired - Fee Related JP5843758B2 (en) 2009-05-29 2010-05-17 Flavonoid hydrogel

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US (3) US8541016B2 (en)
EP (1) EP2435504B1 (en)
JP (2) JP5843758B2 (en)
SG (2) SG176286A1 (en)
WO (1) WO2010138082A1 (en)

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US8722040B2 (en) * 2011-06-24 2014-05-13 Liveleaf, Inc. Site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites
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