JP2016002120A - Epidermis thickness estimation method - Google Patents
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 210000002615 epidermis Anatomy 0.000 title claims abstract description 30
- 210000003491 skin Anatomy 0.000 claims abstract description 94
- 210000004204 blood vessel Anatomy 0.000 claims description 23
- 230000017531 blood circulation Effects 0.000 claims description 10
- 230000003796 beauty Effects 0.000 claims description 8
- 239000002537 cosmetic Substances 0.000 claims description 8
- 230000009759 skin aging Effects 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 description 11
- 230000032683 aging Effects 0.000 description 10
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 4
- 238000012014 optical coherence tomography Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 2
- 229940032094 squalane Drugs 0.000 description 2
- 206010051246 Photodermatosis Diseases 0.000 description 1
- 238000009223 counseling Methods 0.000 description 1
- 238000001739 density measurement Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000008845 photoaging Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
Description
本発明は、表皮厚の推定方法に関する。 The present invention relates to a method for estimating skin thickness.
一般に、表皮厚は、加齢に伴い薄くなっていくことが知られている（非特許文献１）。一方、同年代の人を比較した場合に、表皮が厚い人と薄い人がいるが、この個人差は蓄積した紫外線ダメージ（光老化）により、表皮の菲薄化の程度が異なるためと考えられる。個々の肌状態にあった化粧品を提供するには、肌の老化状態を知る必要があるが、表皮厚は皮膚の加齢による老化の程度や蓄積した紫外線ダメージの程度を知る重要な指標となっている。 In general, it is known that the skin thickness becomes thinner with aging (Non-Patent Document 1). On the other hand, when people of the same age are compared, there are people with thick and thin epidermis, but this individual difference is thought to be due to the degree of thinning of the epidermis due to accumulated ultraviolet damage (photoaging). It is necessary to know the aging state of the skin in order to provide cosmetics that match the individual skin condition, but the epidermis thickness is an important indicator of the degree of aging due to skin aging and the degree of accumulated ultraviolet damage. ing.
表皮厚の測定方法としては、皮膚の切断面を顕微鏡観察する侵襲法がある。また、非侵襲的な表皮厚の測定方法として、共焦点レーザー生体顕微鏡を使用して測定深度を漸次変えて皮膚の水平断面画像を撮る方法、光干渉断層法（ＯＣＴ）等により表皮の層状構造を計測する方法がある（特許文献１）。 As a method for measuring the skin thickness, there is an invasive method in which a cut surface of the skin is observed with a microscope. In addition, as a non-invasive method of measuring the skin thickness, a method of taking a horizontal cross-sectional image of the skin by gradually changing the measurement depth using a confocal laser biomicroscope, a layered structure of the epidermis by optical coherence tomography (OCT), etc. There is a method of measuring (Patent Document 1).
上記表皮厚の測定方法のうち、侵襲法は被験者への負担が大きい。
一方、共焦点レーザー生体顕微鏡、ＯＣＴ等を用いる従来の非侵襲法は装置が高額または大がかりであったり、また、操作に習熟を要する。そのため、簡便には使用することができず、店頭等で行う美容カウンセリングには適さない。
Among the methods for measuring the skin thickness, the invasive method places a heavy burden on the subject.
On the other hand, in the conventional non-invasive method using a confocal laser biomicroscope, OCT, etc., the apparatus is expensive or large-scale, and the operation requires skill. Therefore, it cannot be used conveniently and is not suitable for beauty counseling performed at a store or the like.
これに対し、本発明の課題は、非侵襲的、かつ簡便な方法で表皮厚を推定することに関する。 On the other hand, the subject of the present invention relates to estimating the skin thickness by a non-invasive and simple method.
本発明者は、皮膚表面からマイクロスコープで表皮の画像を撮影すると、血液が流れている毛細血管のみが観察され、毛細血管のうち島状に観察されるループ状の毛細血管の像の分布の密度（以下、ループ状毛細血管密度という）は表皮厚と相関することを見出し、このループ状毛細血管密度に基づいて表皮厚を推定する本発明を想到した。 When the present inventor takes an image of the epidermis with a microscope from the surface of the skin, only capillaries through which blood flows are observed, and the distribution of images of looped capillaries observed in islands among capillaries The present inventors have found that the density (hereinafter referred to as loop capillary density) correlates with the epidermis thickness, and arrived at the present invention for estimating the epidermis thickness based on the loop capillary density.
即ち、本発明は、マイクロスコープで被験者の表皮の画像を撮り、
該画像で観察される、血液が流れているループ状毛細血管像の分布の密度を計測し、複数人から取得されたループ状毛細血管密度と表皮厚との関係に基づき、被験者のループ状毛細血管密度から表皮厚を推定する表皮厚の推定方法を提供する。
That is, the present invention takes an image of a subject's epidermis with a microscope,
The density of the distribution of looped capillary images in which blood flows observed in the image is measured, and based on the relationship between the loop capillary density obtained from multiple persons and the skin thickness, the subject's looped capillaries are measured. Provided is a method for estimating the skin thickness, which estimates the skin thickness from the blood vessel density.
また、本発明は、当該方法により推定した被験者の表皮厚に基づき、被験者の皮膚の老化の程度を評価する方法を提供すると共に、被験者に好ましい化粧料を推奨する美容アドバイス方法を提供する。 In addition, the present invention provides a method for evaluating the degree of skin aging of a subject based on the skin thickness of the subject estimated by the method, and also provides a beauty advice method for recommending a preferred cosmetic to the subject.
本発明によれば、マイクロスコープで非侵襲的に皮膚表面の画像を撮ることにより、被験者の表皮厚をより簡便に推定することができる。 According to the present invention, the skin thickness of a subject can be estimated more simply by taking an image of the skin surface non-invasively with a microscope.
したがって、推定された表皮厚から、公知の表皮厚と老化との関係に基づき、被験者の皮膚の老化の程度を評価することができ、さらに、被験者の皮膚の老化の程度に応じた化粧料を推奨することができる。 Therefore, based on the estimated skin thickness, it is possible to evaluate the degree of skin aging of the subject based on the relationship between the known skin thickness and aging. Can be recommended.
以下、図面を参照しつつ本発明を詳細に説明する。
本発明は、皮膚表面からマイクロスコープで表皮の画像を撮ると、皮膚表面画像内に血液が流れているループ状毛細血管の血管像が島状に観察され、この血液が流れているループ状毛細血管の島状の血管像の分布の密度（即ち、ループ状毛細血管密度）と表皮厚には相関関係があるとの本発明者の知見に基づいている。そこで、本発明においては、予め、ループ状毛細血管密度と表皮厚との関係を取得しておき、一方、任意の被験者についてループ状毛細血管密度を計測し、予め取得しておいたループ状毛細血管密度と表皮厚との関係に基づき、任意の被験者のループ状毛細血管密度からその被験者の表皮厚を推定する。
Hereinafter, the present invention will be described in detail with reference to the drawings.
In the present invention, when an image of the epidermis is taken from the skin surface with a microscope, a blood vessel image of a looped capillary blood flowing in the skin surface image is observed in an island shape, and the looped capillary in which the blood flows is observed. This is based on the knowledge of the present inventor that there is a correlation between the density of the distribution of blood vessel island-like blood vessel images (that is, the density of looped capillaries) and the skin thickness. Therefore, in the present invention, the relationship between the loop capillary density and the epidermis thickness is acquired in advance, while the loop capillary density is measured for any subject, and the loop capillary acquired in advance is measured. Based on the relationship between the blood vessel density and the skin thickness, the skin thickness of the subject is estimated from the loop capillary density of any subject.
＜ループ状毛細血管密度と表皮厚との関係の取得＞
ループ状毛細血管密度と表皮厚との関係は、次のようにして得ることができる。
まず、複数人の被験者について、表皮厚を推定する皮膚の表面にマイクロスコープを当て、皮膚表面の画像を撮る。この場合、表皮厚を推定する皮膚の部位は、マイクロスコープにより、表皮内のループ状毛細血管の血管像を撮れる部位であれば良く、例えば、顔、腕、手甲等とすることができる。
<Acquisition of relationship between loop capillary density and skin thickness>
The relationship between the loop capillary density and the skin thickness can be obtained as follows.
First, for a plurality of subjects, a microscope is applied to the surface of the skin where the skin thickness is estimated, and an image of the skin surface is taken. In this case, the skin part for estimating the epidermis thickness may be a part where a blood vessel image of a looped capillary blood vessel in the epidermis can be taken with a microscope, and can be, for example, the face, arm, back of the hand, or the like.
マイクロスコープとしては、５０〜６００倍で動画を撮ることのできるものが好ましい。例えば、株式会社徳製、ＢＳｃａｎを使用することができる。真皮乳頭の深さの血管像を撮るため、皮膚表面から深さ１０μｍ〜１００μｍの位置の血管像を撮ることが好ましい。 As the microscope, a microscope capable of taking a moving picture at 50 to 600 times is preferable. For example, Toku Co., Ltd. and BSscan can be used. In order to take a blood vessel image at the depth of the dermal papilla, it is preferable to take a blood vessel image at a depth of 10 μm to 100 μm from the skin surface.
また、表皮の撮像時には、皮膚表面に、水又は、スクワラン、オリーブオイル等の油脂を塗布し、それにより表面反射を抑制して皮膚内部の画像を撮り易くすることが好ましい。 Further, when imaging the epidermis, it is preferable to apply water or oils such as squalane and olive oil to the skin surface, thereby suppressing surface reflection and making it easy to take an image of the inside of the skin.
皮膚表面を広い面積で撮影する場合には、撮影プローブをゆっくりと動かし、所定時間の動画を撮影してもよい。 When photographing the skin surface over a wide area, the photographing probe may be moved slowly to photograph a moving image for a predetermined time.
こうして撮った皮膚表面画像では、例えば、図１Ａに示すように、真皮乳頭に存在するループ状毛細血管の血管像１が島状に分散して見える。血管像１は、撮影画像が動画の場合も静止画の場合も、毛細血管中を流れる赤血球の色や周辺部とのコントラストによって、血液が流れている血管を識別することができる。特に、動画で観察すると、血流の有無が容易にわかる。 In the skin surface image thus taken, for example, as shown in FIG. 1A, the blood vessel image 1 of the loop-like capillaries existing in the dermal papilla appears to be dispersed in an island shape. Whether the captured image is a moving image or a still image, the blood vessel image 1 can identify a blood vessel in which blood flows by the color of red blood cells flowing in the capillary and the contrast with the peripheral portion. In particular, the presence or absence of blood flow can be easily understood by observing with moving images.
図１Ｂは、図１Ａに示した皮膚表面画像において、血流のあるループ状毛細血管の血管像１を点線２で囲んだものである。 FIG. 1B shows a blood vessel image 1 of a looped capillary with blood flow surrounded by a dotted line 2 in the skin surface image shown in FIG. 1A.
次に、こうして得た皮膚表面画像のループ状毛細血管密度を計測する。ループ状毛細血管密度は、血液が流れているループ状毛細血管像の分布の密度であるから、端的には、このループ状毛細血管像の皮膚単位面積あたりの個数として計測される。かかる個数をフォトスケールを用いて段階的に計測してもよい。フォトスケールを用いる場合に、フォトスケールのスコア（以下、血管密度スコアという）で評価してもよい。 Next, the loop capillary density of the skin surface image thus obtained is measured. Since the loop capillary density is the density of the distribution of loop capillary images through which blood flows, it is simply measured as the number of loop capillary images per unit skin area. Such a number may be measured step by step using a photoscale. When using a photoscale, evaluation may be performed using a photoscale score (hereinafter referred to as a blood vessel density score).
以上の皮膚表面画像の取得とループ状毛細血管密度の計測は、具体的には、例えば以下の順序で行う。なお、撮影した個々の皮膚表面画像におけるループ状毛細血管密度の計測を複数名の評価者で図２に示すようなフォトスケールを用いて行い、評価者の血管密度スコアを平均化したものをループ状毛細血管密度の計測値として用いてもよい。 Specifically, the acquisition of the skin surface image and the measurement of the loop capillary density are performed in the following order, for example. In addition, measurement of loop capillary density in each photographed skin surface image is performed by a plurality of evaluators using a photo scale as shown in FIG. 2, and an average of the evaluator's blood vessel density score is looped. It may be used as a measurement value of the capillary density.
工程１：予めループ状毛細血管密度が段階的に異なる複数のループ状毛細血管画像をフォトスケールとして作成しておく（図２）。
この場合、フォトスケールの作成は次の手順で行う。まず、２０代〜６０代の複数人の額、目元、頬、口元、手の各部を動画撮影する。撮影画像を、ループ状毛細血管密度によって次のようにスコア１〜５の５段階に分け、それぞれのスコアを代表する動画中から平均的なループ状毛細血管密度の静止画を選択し、各段階の基準画像とする。
スコア１：ほとんどない（ループ状毛細血管の島状の血管像１個／０．３５ｍｍ2未満）、
スコア２：わずかにある（ループ状毛細血管の島状の血管像１個／０．３５ｍｍ2以上５個／０．３５ｍｍ2未満）、
スコア３：中程度にある（ループ状毛細血管の島状の血管像５個／０．３５ｍｍ2以上１０個／０．３５ｍｍ2未満）、
スコア４：やや多い（ループ状毛細血管の島状の血管像１０個／０．３５ｍｍ2以上１５個／０．３５ｍｍ2未満）、
スコア５：多い（ループ状毛細血管の島状の血管像１５個／０．３５ｍｍ2以上）
Step 1: A plurality of loop-shaped capillary images having different loop-shaped capillary densities in steps are created in advance as a photo scale (FIG. 2).
In this case, the photo scale is created according to the following procedure. First, a movie is taken of the forehead, eyes, cheeks, mouth, and hands of multiple people in their 20s to 60s. The photographed image is divided into five stages of scores 1 to 5 as follows according to the loop capillary density, and a still image of the average loop capillary density is selected from videos representing each score. As a reference image.
Score 1: almost no (one island-like blood vessel image of looped capillaries / less than 0.35 mm 2 )
Score 2: Slightly (1 island-like blood vessel image of looped capillaries / 0.35 mm 2 or more and less than 5 / 0.35 mm 2 )
Score 3: Medium (5 island images of looped capillaries / 0.35 mm 2 or more and less than 10 / 0.35 mm 2 )
Score 4: Slightly more (loop island blood vessel image 10 / 0.35 mm 2 or more but less than 15 / 0.35 mm 2 ),
Score 5: Many (15 island images of looped capillaries / 0.35 mm 2 or more)
工程２：フォトスケールの作成のための撮影とは別に、複数人の一定面積の皮膚表面を位置をずらしながら約２分間動画を撮影する。 Step 2: Separately from photographing for creating a photoscale, a moving image is photographed for about 2 minutes while shifting the positions of the skin surfaces of a certain area of a plurality of people.
工程３：工程２で撮影した動画について、撮像視野（０．３５ｍｍ2）におけるループ状毛細血管密度が平均的な皮膚表面画像を定め、その皮膚表面画像のループ状毛細血管密度を、工程１で作成した５段階のスコアのフォトスケールを用いて段階的に計測する。
なお、フォトスケールを用いるループ状毛細血管密度の計測では、各スコアの基準画像に基づいて目視により段階的スコアでループ状毛細血管密度を計測するため、当該皮膚表面画像におけるループ状毛細血管密度が、或るスコアの基準画像と、その次のスコアの基準画像の間にあると判断される場合、隣り合うスコアの間に中間のスコアを設定し、５つの基準画像を用いる９段階のスコアでループ状毛細血管密度を計測してもよい。
Step 3: For the moving image taken in Step 2, an average skin surface image having a loop capillary density in the imaging field of view (0.35 mm 2 ) is determined, and the loop capillary density of the skin surface image is determined in Step 1 Measurements are made step by step using the photo scale of the created five-step score.
In the measurement of the loop capillary density using a photoscale, the loop capillary density in the skin surface image is determined because the loop capillary density is visually measured with a stepwise score based on the reference image of each score. If it is determined that the current image is between the reference image of a certain score and the reference image of the next score, an intermediate score is set between adjacent scores, and the score is 9 levels using five reference images. The loop capillary density may be measured.
ループ状毛細血管密度を計測する手法としては、フォトスケールを用いて段階的に血管密度スコアで計測するほかに、血流のあるループ状毛細血管の血管像の個数を計測し、皮膚表面画像の単位面積当たりの、血流のあるループ状毛細血管の血管像の個数（即ち、ループ状毛細血管密度［個／ｍｍ2］）を求める。このループ状毛細血管密度の具体的な計測方法としては、例えば、計測範囲をマイクロスコープの視野範囲全体とし、その中に確認できるループ状毛細血管の個数を数えて、単位面積あたりの個数を算出することができる。
この他、ループ状毛細血管密度は、画像処理により計測してもよい。
As a method of measuring the density of looped capillaries, in addition to measuring the blood vessel density score step by step using a photoscale, the number of blood vessel images of looped capillaries with blood flow is measured, and the skin surface image is measured. The number of blood vessel images of looped capillaries with blood flow per unit area (that is, loop capillaries density [number / mm 2 ]) is obtained. As a specific method for measuring the density of loop capillaries, for example, the measurement range is the entire field of view of the microscope, and the number of loop capillaries that can be confirmed is counted to calculate the number per unit area. can do.
In addition, the loop capillary density may be measured by image processing.
一方、ループ状毛細血管密度を計測した皮膚領域について、表皮厚を求める。表皮厚の測定方法としては、例えば、Ｉｎ ｖｉｖｏ生体共焦点レーザー顕微鏡（例えば、株式会社インテグラル製、Ｖｉｖａｓｃｏｐｅ）で水平断面画像を非侵襲的に撮影し、表皮厚を測定する領域内で表皮構造が完全に見えなくなる深さを求める。 On the other hand, the skin thickness is obtained for the skin region where the loop capillary density is measured. As a method for measuring the skin thickness, for example, a horizontal cross-sectional image is taken non-invasively with an in vivo confocal laser microscope (for example, Vivascope manufactured by Integral Co., Ltd.), and the skin structure is measured in the region where the skin thickness is measured. Find the depth at which you can no longer see.
こうして得られるループ状毛細血管密度と、表皮厚とは、例えば、６０歳以上６９歳以下の老齢者を被験者とした場合に、図３に示すように相関を有している。なお、このループ状毛細血管密度の計測では図２に示すフォトスケールを使用したが、計測されたループ状毛細血管密度の殆どがスコア２以下であったため、スコア１とスコア２の間にスコア１．５の中間値を設定した。
なお、図３に示した、ループ状毛細血管密度と表皮厚との関係は、Ｓｐｅａｒｍａｎの順位相関検定を用いて検証することにより、ｐ＜０．０５で有意な相関関係があることがわかった。
The loop-shaped capillary density obtained in this way and the skin thickness have a correlation as shown in FIG. 3, for example, when an elderly person 60 years old or older and 69 years old or younger is a subject. In the measurement of the loop capillary density, the photoscale shown in FIG. 2 was used. However, since most of the measured loop capillary density was less than or equal to score 2, score 1 between score 1 and score 2 An intermediate value of .5 was set.
The relationship between the loop capillary density and the epidermis thickness shown in FIG. 3 was verified by using Spearman's rank correlation test, and it was found that there was a significant correlation at p <0.05. .
また、この複数人は同一年代か、年齢が大きく異ならない範囲の被験者集合とすることが好ましい。 In addition, it is preferable that the plurality of persons be the same age group or a set of subjects whose age does not vary greatly.
＜任意の被験者の表皮厚の推定＞
任意の被験者のループ状血管密度を上述と同様に行い、上述の相関関係に基づいて該被験者の表皮厚を推定する。そのため、ループ状毛細血管密度と表皮厚との回帰式を求めておくことが好ましい。この場合、任意の被験者の年齢は、ループ状毛細血管密度と表皮厚との関係を求めたときの年齢幅に入るようにすることが好ましい。
<Estimation of skin thickness of any subject>
The loop blood vessel density of an arbitrary subject is performed in the same manner as described above, and the skin thickness of the subject is estimated based on the above-described correlation. Therefore, it is preferable to obtain a regression equation between the loop capillary density and the skin thickness. In this case, it is preferable that the age of any subject falls within the age range when the relationship between the loop capillary density and the skin thickness is obtained.
＜表皮厚に基づく美容アドバイス＞
本発明の方法により推定された被験者の表皮厚は、個々の被験者ごとに表皮の老化の程度の指標となることがわかっている。
そこで、美容アドバイザーは、例えば、図４に示すように、(1)現在の被験者の皮膚表面画像と、(2)その画像のループ状毛細血管密度から推定される表皮厚を示すと共に、(3)現在よりも皮膚の代謝が活発化し、肌年齢が若くなった場合の皮膚表面画像の予想図と、(4)現在よりも皮膚の代謝が衰え、肌年齢が増した場合の皮膚表面画像の予想図を示す。この画像の提示は、肌年齢を現在よりも若くしたいと希望している被験者にとって、肌の適切な管理を行うことに対する強い動機付けになる。美容アドバイザーは、表皮の老化の進行の程度に応じて、皮膚に適用すべき化粧料や美容上の肌の手入れ方法を選択することができるから、被験者に好ましい化粧料を推奨し、美容上の肌の手入れ方法を説明する美容アドバイスを行うことができる。この場合、加齢による老化、あるいは表皮の老化の程度と、老化に応じて使用すべき化粧料について、予めデータベースを構築してもよい。
<Beauty advice based on skin thickness>
It has been found that the subject's epidermis thickness estimated by the method of the present invention is an indicator of the degree of epidermis aging for each subject.
Therefore, for example, as shown in FIG. 4, the beauty advisor shows (1) the skin surface image of the current subject and (2) the skin thickness estimated from the loop capillary density of the image, and (3 ) Expected skin surface image when skin metabolism is activated and skin age is younger than (4), and (4) Skin surface image when skin metabolism declines and skin age is higher than current An expected figure is shown. The presentation of this image is a strong motivation for the appropriate management of the skin for the subject who wants to make the skin age younger than the present age. Beauty advisors can select cosmetics to be applied to the skin and cosmetic skin care methods according to the degree of progress of aging of the epidermis. Beauty advice explaining how to care for the skin can be given. In this case, a database may be constructed in advance for the degree of aging due to aging or the degree of aging of the epidermis and the cosmetics to be used according to aging.
以下、実施例に，基づいて本発明を詳細に説明する。
（１）ループ状毛細血管密度と表皮厚との関係の取得
(1-1) ループ状毛細血管密度の計測
６０歳から６８歳の女性２０名の被験者の頬にスクワランを滴下し、その上からビデオマイクロスコープ（ＢＳｃａｎ、株式会社徳製）を軽く押し立て、ゆっくりと測定位置を動かしながら、約２分間皮膚表面の動画を取得した。
Hereinafter, the present invention will be described in detail based on examples.
(1) Acquisition of relationship between loop capillary density and skin thickness
(1-1) Loop capillary density measurement Squalane was dropped on the cheeks of 20 female subjects aged 60 to 68 years, and a video microscope (BSscan, manufactured by Toku Co., Ltd.) was lightly pushed up. A moving image of the skin surface was acquired for about 2 minutes while slowly moving the measurement position.
こうして得られた皮膚表面画像に対し、予め２０代から６０代の女性被験者９０名を対象として撮影された毛細血管画像を用いて作成した図２に示す５段階のフォトスケールを用いてスコア付を行った。 The skin surface image obtained in this way is scored using a five-stage photoscale shown in FIG. 2, which was created using capillary images previously taken for 90 female subjects in their 20s to 60s. went.
(1-2)表皮厚の計測
Ｉｎ ｖｉｖｏ 生体共焦点レーザー顕微鏡（株式会社インテグラル製、Ｖｉｖａｓｃｏｐｅ １５００）を用い、頬部の皮膚の水平断面画像を角層表面より３．１７μｍ刻みで約１５０μｍ深度まで計測し、表皮層と真皮層の境界部が見え始めた深さを表皮厚上端とし、境界部が完全に見えなくなる深さを表皮厚下端とし、表皮厚上端と下端の平均値を表皮厚とした。被験者１名につき頬部３か所を計測し、３か所の表皮厚の平均値を求めた。
(1-2) Measurement of epidermis thickness Using an in vivo biological confocal laser microscope (Integra Inc., Vivascope 1500), a horizontal cross-sectional image of the cheek skin from the stratum corneum surface to a depth of about 150 μm in 3.17 μm increments. The depth at which the boundary between the epidermis layer and the dermis layer begins to be visible is taken as the upper end of the skin thickness, the depth at which the border is completely invisible is taken as the lower end of the skin thickness, and the average value of the upper and lower skin thicknesses It was. Three cheeks were measured for each subject, and the average value of the skin thickness at the three locations was determined.
(1-3)ループ状毛細血管密度と表皮厚との関係
(1-1)で得たループ状毛細血管密度Ｘと(1-2)で得た表皮厚Ｙとから次の回帰式を得た。
Ｙ＝７．９１０５Ｘ＋２４．０４８
（Ｒ＝０．５４３、ｐ＜０．０５）
(1-3) Relationship between loop capillary density and skin thickness
The following regression equation was obtained from the loop capillary density X obtained in (1-1) and the skin thickness Y obtained in (1-2).
Y = 7.9105X + 24.048
(R = 0.543, p <0.05)
（２）被験者の表皮厚の推定
３７歳の女性を被験者とし、被験者のループ状毛細血管密度を上述の(1-1)と同様に計測した。また、この被験者の皮膚表面画像のループ状毛細血管密度は、(1-1)で得たフォトスケールによれば、フォトスケールの評価スコアは「２」であった。
(2) Estimation of subject's epidermis thickness A 37-year-old female was used as a subject, and the subject's loop capillary density was measured in the same manner as in (1-1) above. Further, according to the photoscale obtained in (1-1), the photoscale evaluation score of the loop capillary density in the skin surface image of this test subject was “2”.
(1-3)で得た回帰式に基づき、ループ状毛細血管密度の計測値から表皮厚を推定したところ、３９．９μｍであった。
一方、この被験者のループ状毛細血管密度の計測部位の表皮厚を、(1-2)と同様にして共焦点レーザー顕微鏡で測定したところ、４１．２μｍであり、皮膚表面画像から表皮厚を良好に推定することができた。
Based on the regression equation obtained in (1-3), the skin thickness was estimated from the measured value of the loop capillary density, and was 39.9 μm.
On the other hand, when the skin thickness of the measurement site of the loop capillary density of this test subject was measured with a confocal laser microscope in the same manner as in (1-2), it was 41.2 μm, and the skin thickness was good from the skin surface image. Could be estimated.
１ 血管像 1 Blood vessel image
Claims (7)
該画像で観察される、血液が流れているループ状毛細血管像の分布の密度を計測し、複数人から取得されたループ状毛細血管密度と表皮厚との関係に基づき、被験者のループ状毛細血管密度から表皮厚を推定する表皮厚の推定方法。 Take an image of the subject's epidermis with a microscope,
The density of the distribution of looped capillary images in which blood flows observed in the image is measured, and based on the relationship between the loop capillary density obtained from multiple persons and the skin thickness, the subject's looped capillaries are measured. A method for estimating the skin thickness, which estimates the skin thickness from the blood vessel density.
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| JP2019025071A (en) * | 2017-07-31 | 2019-02-21 | 花王株式会社 | Skin condition evaluation method and skin condition evaluation device |
| JP2019058641A (en) * | 2017-09-22 | 2019-04-18 | 富士フイルム株式会社 | Skin inner structure estimation method, skin inner structure estimation program, and skin inner structure estimation device |
| WO2021090917A1 (en) * | 2019-11-08 | 2021-05-14 | 株式会社資生堂 | Method for determining discoloration attributes, system for determining discoloration attributes, method for estimating vascular density, and system for estimating vascular density |
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