JP2015524070A5 - - Google Patents

Claims (25)

In the detection system of particles flowing in a number of the flow stream,
An array of sample streams consisting of a number of flow streams generated by an acoustic transducer ;
A light beam configured to analyze previous SL multiple flow streams, the light beam traverses the space and the number of flow streams interposed between streams, Ru allowed and generate a spatially continuous excitation and,
A optical objective lens for focusing the emitted light of the excited particles flowing in the sample stream, said optical objective lens for forming the outgoing light array detector,
A system comprising:
The outgoing light from each of the flow streams is individually identified, and the outgoing light from any other flow stream is the outgoing light differentiated by the position at the array detector. Detection system.
Furthermore, the detection system of claim 1, in a single channel including at least two streams in the array of the sample stream, with at least one channel.
Detection system according to claim 2 for generating an acoustic wave in the within this channel is provided an acoustic transducer within the range is sufficiently close to said channel.
The detection system according to claim 2, wherein the channel is formed in a substrate.
The detection system according to claim 4 , wherein the acoustic transducer is connected to the base body.
The detection system according to claim 5 , wherein a second acoustic transducer is connected to the base body.
Detection system according to claim 2 having a large number of channels.
The detection system according to claim 4 , wherein the substrate has a window, and a light beam incident through the window traverses all of the sample streams in the array of sample streams.
Detection system according to claim 8 wherein the light beam is a Bessel beam.
The detection system according to claim 4 , wherein at least a part of the substrate is formed of an optically transparent material.
The detection system of claim 1 , wherein the array detector is a PMT array and each flow stream correlates to an individual PMT.
The detection system of claim 1 , wherein the array detector is a digital camera and each flow stream correlates to a pixel location within the camera.
The detection system of claim 1 , further comprising one or more telescopes for adjusting the size of the optical path.
The detection system of claim 1 , wherein the array detector further comprises a mask.
In a method for detecting particles flowing in multiple sample streams,
Run the sample containing the particles through a number of sample streams generated by an acoustic transducer ,
Guide light across a number of sample streams thereof by the light, the sample also in the space intervening between streams by generating a spatially continuous excitation across all sample stream including,
Collecting the output light of the sample stream by the excitation of the particles flowing into the sample stream , directing the output light towards a detector; and
Method characterized in that the light emitted from each flow stream, the light emitted from any other flow streams together when individually identified and differentiated.
Before SL multiple sample stream flows through the channel, and further by the incidence of the acoustic wave field through the channel, The method according to claim 15 for restraining the path of the particles in the sample.
The sample is considered to contain the target of interest, and the sample is further attached to a plurality of negative acoustics to which the biomolecule is attached under conditions sufficient for a functionally stable biomolecule to capture the target. 17. The method of claim 16 , wherein the acoustic wave field comprises exposing to contrast particles and the acoustic wave field comprises focusing the negative acoustic contrast particles to a maximum pressure potential.
The method of claim 17 , wherein the acoustic standing wave generates a number of nodes, each node being a discrete sample stream.

In a detection system for particles in multiple flow streams,
At least one channel containing a number of flow streams generated by an acoustic transducer;
A light beam configured to analyze the multiple flow streams, wherein the light beam generates spatially continuous excitation across all flow streams, including within the space interposed between the flow streams; ,
An optical objective that collects light from the excited particles flowing in the sample stream, the optical objective for imaging the light onto an array detector;
A system comprising:
The outgoing light from each of the flow streams is individually identified, and the outgoing light from any other flow stream is the outgoing light differentiated by the position at the array detector. Detection system.
The detection system of claim 19, further comprising a number of channels, each channel having two or more of the flow streams .
The detection system according to claim 1, wherein background information is collected from the intervening space .
The method according to claim 15, further collecting light emitted from the intervening space between the streams.
The method of claim 19, collecting background information from the intervening space .
The detection system according to claim 1, wherein particles are analyzed in a flow shape of the multiple flow streams .
The method of claim 15, wherein the excited particles flow in the flow shape of the flow stream.