JP2015226595A - Probe fixture and probe for insertion into living body - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a probe fixture enabling a probe body to be easily fixed to a guide cannula without a position deviation of a probe tip, as well as a probe for insertion into a living body.SOLUTION: A probe fixture 2 includes: a probe housing 10; a guide cannula 30 having a housing space 30a inside; and a stopper 40 that is mounted to the probe housing 10 and can fix the probe housing 10 to the guide cannula 30. The guide cannula 30 includes an engaging projection 31 projecting in a direction intersecting an insertion direction B1-B2 of the probe housing 10 into the housing space 30a. The stopper 40 includes an engaging pawl 43 that can be displaced in a direction intersecting the insertion direction B1-B2. When the probe housing 10 is inserted into the housing space 30a with the stopper 40 being mounted to the probe housing 10, the engaging pawl 43 is displaced to engage with the engaging projection 31.

Description

  The present invention relates to a probe fixture and a biological insertion probe, and more particularly to a probe fixture and a biological insertion probe that can be used for microdialysis and the like.

  In the medical and research fields, fine elements are embedded in laboratory animals and humans to detect substances in the living body. For example, the microdialysis method is a method for detecting and quantifying a substance that has permeated through a dialysis membrane by inserting a probe having a microdialysis membrane at the tip into a living body. In the microdialysis method (microdialysis method), an endogenous source derived from a living body or an external factor such as an administered drug from an unanesthetized and unrestrained animal in an extracellular fluid of a living tissue or organ, blood, or cerebrospinal fluid. Sampling of sex substances can be performed with minimal invasiveness.

  For example, by inserting a probe into the brain of a laboratory animal and performing a microdialysis method, by continuously sampling neurotransmitters in a specific region in the brain, it is possible to evaluate the efficacy of central drugs, etc. it can. The microdialysis method can also be used for monitoring pharmacokinetics and energy metabolism in local tissues.

  Furthermore, in recent years, a technique has also been developed in which a minute biosensor is embedded in a living body and a substance in the living body is monitored by a chemical or electrical technique. As such a micro biosensor, for example, a glucose sensor in which a fine electrode chip is produced using a semiconductor microfabrication technique such as photolithography is known.

  Conventionally, in the microdialysis method, a technique for fixing a dialysis probe to a guide cannula using a cap nut is known (see Patent Document 1). According to this conventional technique, thread grooves are formed in the outer periphery of the guide cannula and the inner periphery of the cap nut. Then, after the dialysis probe is inserted into the guide tube of the guide cannula, the dialysis probe is fixed between the guide cannula and the cap nut by screwing the cap nut into the guide cannula.

Japanese Patent Laid-Open No. 7-265317 (paragraphs 0022 to 0027, FIGS. 1 to 3 etc.)

  In this conventional technique, after the dialysis probe is once inserted into the guide cannula, a cap nut is further attached to the guide cannula and then rotated and screwed. When the cap nut is rotated, the dialysis probe enters toward the distal end side of the guide cannula, so that the distal end of the dialysis probe is further pushed into the living body. As a result, even if the tip of the dialysis probe can be placed at the desired position on the living tissue before it is fixed with the cap nut, the tip position of the dialysis probe can be changed to the desired position by attaching the cap nut to the guide cannula and rotating it. May deviate from. Further, the tip of the dialysis probe is pushed into the back by the rotation of the cap nut, and the living tissue may be damaged by the probe tip. Further, since the dialysis probe and the cap nut used in the microdialysis method are very small structures, it is difficult to rotate the cap nut by hand, and there is a problem that workability is poor.

  SUMMARY OF THE INVENTION An object of the present invention is to provide a probe fixture and a living body insertion probe capable of easily fixing a probe main body to a guide cannula without causing positional deviation of the probe tip.

  According to the probe fixture of the present invention, the above-described problems are a probe container that can accommodate a probe assembly, a guide cannula that has an accommodation space that can be accommodated by inserting the probe container, and the probe container. And a stopper capable of fixing the probe container accommodated in the accommodation space to the guide cannula, and either one of the guide cannula and the stopper is the one of the probe container. An engaging projection protruding in a direction intersecting with the insertion direction into the accommodating space, and the other of the guide cannula and the stopper has an engaging claw displaceable in the direction intersecting with the insertion direction. And when the probe holder is inserted into the receiving space with the stopper attached to the probe holder. , Wherein the engagement claw engages the displaced the engaging projection.

  Thus, according to the present invention, the stopper can be attached to the probe container, and the engagement protrusion and the engagement claw are engaged when the probe container is inserted into the accommodation space. If the stopper is attached to the probe holder in advance and inserted into the guide cannula in this state, the engaging protrusion and the engaging claw are engaged with each other so that the probe holder is not attached to the probe holder. It becomes possible to fix to the guide cannula. Therefore, there is no need to perform operations such as rotating the stopper to fix the probe, and the probe holder can be easily fixed to the guide cannula by inserting it into the guide cannula with the probe holder and stopper attached. can do.

  Furthermore, the probe container has an engagement recess formed in a side surface formed with a groove extending along a direction perpendicular to the direction in which the engagement protrusion protrudes, and the stopper has a shape corresponding to the engagement recess. It is preferable that it is possible to detachably attach to the probe container by inserting the engaging recess into the notch in the vertical direction.

  Thus, since the engagement recess is formed in the probe container and the stopper has a notch, the stopper can be inserted in a direction perpendicular to the direction in which the engagement protrusion protrudes and attached to the probe container. Thereby, after use, it becomes possible to remove the stopper by sliding it in a direction perpendicular to the direction in which the engaging projection protrudes. Therefore, the fixation of the probe container with the stopper can be easily released.

  Further, the engaging claw includes a bent portion that extends in the insertion direction and can be elastically deformed on the base end side, and the bent portion is elastically deformed in a direction intersecting the insertion direction to displace the engaging claw. It is preferable to do.

  As described above, since the bent portion is elastically deformed and the engaging claw is displaced, the stopper can be easily engaged with the guide cannula by pushing the stopper toward the guide cannula.

Furthermore, the engagement protrusion has a first inclined portion whose diameter is enlarged on the tip side in the insertion direction,
It is preferable that the engaging claw has a second inclined portion whose diameter is reduced on the tip side in the insertion direction.

  In this way, the distal end side of the engaging protrusion is enlarged, and the distal end side of the engaging claw is reduced in diameter. That is, the engaging protrusion and the tip of the engaging claw are inclined in the same direction with respect to the insertion direction. Therefore, when the stopper is pushed in the insertion direction, the engaging claw enters the lower side of the engaging protrusion and engages so as to slide between the inclined part of the engaging protrusion and the inclined part of the engaging claw. Thereby, a probe container can be smoothly attached to a guide cannula.

  In addition, one of the probe container and the guide cannula includes a protruding portion that protrudes from the surface, and the other of the probe container and the guide cannula inserts the probe container into the accommodation space. It is preferable to provide an engagement hole formed at a position corresponding to the raised portion.

  Thus, by engaging the raised portion with the engagement hole, the probe container can be securely fixed by the guide cannula.

  According to the living body insertion probe of the present invention, the above object includes the probe fixing tool described in any one of the above and a probe assembly housed in the probe housing tool.

  As described above, according to the present invention, there is no need to perform an operation such as rotating the stopper to fix the probe, and the probe can be accommodated by inserting the probe container and the stopper into the guide cannula. The probe assembly contained in the device can be easily fixed to the guide cannula.

  According to the present invention, it is possible to provide a probe fixing tool and a living body insertion probe that are less likely to be displaced at the probe tip and can easily fix the probe body to the guide cannula.

It is the perspective view which showed the probe fixing tool which concerns on this embodiment. It is the perspective view which exploded and showed the probe assembly. It is the perspective view which showed a mode that the probe assembly was assembled | attached to a probe container. It is the perspective view which showed the procedure which fixes a probe main body to a guide cannula. It is sectional drawing which showed a mode that the engagement nail | claw was displaced and engaged with an engagement protrusion.

  Hereinafter, a plurality of embodiments of the present invention will be described with reference to the drawings. In addition, this invention is not limited by the member, material, etc. which are demonstrated below, These members etc. can be suitably changed in accordance with the meaning of this invention.

  Hereinafter, a probe fixture and a biological insertion probe according to an embodiment of the present invention will be described. In the present embodiment, a microdialysis probe (also referred to as a microdialysis probe) will be described as an example of a living body insertion probe. The microdialysis probe is a probe for detecting a substance (for example, a drug administered in the brain such as a medicinal drug or adrenaline) that has been inserted into an in vivo organ such as a brain of a laboratory animal such as a mouse and passed through a dialysis membrane. . In the following example, a procedure for fixing a microdialysis probe to a mouse brain will be described as an example.

(1) Probe fixture 2
FIG. 1 is a perspective view showing a probe fixture 2 of the present embodiment. As shown in this figure, the probe fixture 2 is inserted into a probe receptacle 10 that can accommodate and fix the probe assembly 20, a guide cannula 30 into which the probe receptacle 10 can be inserted, and a guide cannula 30. And a stopper 40 for fixing the probe container 10 to the guide cannula 30 and preventing the probe container 10 from coming out of the guide cannula 30. Further, as shown in FIG. 3B described later, the probe main body 3 includes a probe container 10 and a probe assembly 20 that is housed in the probe container 10 and includes a dialysis membrane at the tip.

  In addition, the downward direction of FIG. 1 is the same direction as the direction (B1-B2 direction of FIG.4 (b)) which inserts the probe container 10 in the guide cannula 30. FIG. That is, in this specification, “downward direction” and “insertion direction” indicate the same direction. Further, the upward direction means a direction B2-B1 opposite to the insertion direction B1-B2. In addition, the surface facing diagonally lower right in this figure is the front.

(2) Probe container 10
As shown in FIG. 1, the probe container 10 has a substantially pentagonal front shape, and has a shape that is tapered from one end surface 10a downward in a tapered shape. A groove capable of accommodating the probe assembly 20 is formed on the back side of the probe container 10. This groove includes an introduction tube insertion groove 10b extending obliquely from a position near one side surface (right side surface in the figure) of the end surface 10a to the vicinity of the center of the probe container 10, and a side surface on the opposite side of the end surface 10a (FIG. , The lead tube insertion groove 10c extending obliquely from the position near the left side) to the vicinity of the center of the probe housing 10, and the distal side tube insertion groove 10d extending straight downward from the vicinity of the center of the probe storage 10 to the lower end. And is composed of. The tube insertion grooves 10b, 10c, and 10d communicate with each other near the center of the probe container 10.

  A pair of corner portions 11 projecting in the left and right width directions are formed on the end surface 10 a of the probe container 10. On the lower side surface of each corner 11, an engagement recess 12 is formed that is recessed inward in the width direction from the tip of the corner 11. The engaging recess 12 is a groove having a U-shaped longitudinal section extending from the front surface to the back surface of the probe container 10, and both the front side and the back surface side of the probe container 10 are open ends. The groove of the engagement recess 12 is formed in a direction perpendicular to the direction in which the engagement protrusion 31 protrudes when the probe container 10 is inserted into the guide cannula 30 as will be described later.

  A vertical surface 13 extending downward is formed below the engagement recess 12. An inclined surface 14 is formed on the lower side of the vertical surface 13 in a diagonally downward direction. Further, a tip convex portion 15 protruding downward is formed below the inclined surface 14. Further, a hemispherical bulge 16 protruding from the plane is formed near the center of the plane of the probe container 10 (near the tube insertion grooves 10b, 10c, 10d communicate).

  The material of the probe container 10 is not particularly limited, and examples thereof include resins such as polyethylene, polytetrafluoroethylene, polypropylene, polycarbonate, polyacrylate, polymethyl methacrylate, and silicone.

(2) Probe assembly 20
The probe assembly 20 is an assembly having a function that a distal end side is inserted into a living body and a substance in the living body is dialyzed and delivered to the outside. FIG. 2A is a perspective view of the probe assembly 20. As shown in this figure, the probe assembly 20 includes an inner introduction tube 21 that introduces fluid from the outside to the living tissue side, an inner outlet tube 22 that guides the dialyzed fluid from the living tissue to the outside, and an inner introduction tube. An outer introduction tube 23 for fixing 21 to the probe container 10, an outer lead tube 24 for fixing the inner lead tube 22 to the probe container 10, and the distal end side of the inner lead tube 21 to the probe container 10. An outer distal end tube 25 for fixing and a membrane 26 for dialysis of a substance from a living tissue are constituted.

  The inner introduction tube 21 is a tube for introducing a fluid into the distal end side of the probe assembly 20 and is a thin tubular member. The inner outlet tube 22 is a tube for leading the fluid on the distal end side to the outside, and this is also a thin tubular member. The outer introduction tube 23 is a tubular member, the inner diameter thereof is larger than that of the inner introduction tube 21, and the outer diameter is substantially the same as the width of the introduction tube insertion groove 10 b of the probe container 10. The outer lead-out tube 24 is also a tubular member, the inner diameter thereof is larger than that of the inner lead-out tube 22, and the outer diameter is substantially the same as the width of the lead-out tube insertion groove 10 c of the probe container 10.

  Similarly, the outer tip tube 25 is a tubular member having an inner diameter larger than that of the inner introduction tube 21 and an outer diameter substantially equal to the width of the tip side tube insertion groove 10 d of the probe container 10. The membrane 26 is a hollow cylindrical member with a closed end, and is composed of a dialysis membrane. The outer introduction tube 23 is attached to the outside of the inner introduction tube 21, and the outer lead-out tube 24 is attached to the outside of the inner lead-out tube 22. The membrane 26 is attached to the distal end side of the outer distal tube 25. The leading edge of the inner introduction tube 21 is located on the distal end side inside the membrane 26, and the leading edge of the inner lead-out tube 22 is located in the middle of the outer distal tube 25.

  Although the material of these members is not particularly limited, examples of the material of the inner introduction tube 21 and the inner outlet tube 22 include resins such as polyethylene, polycarbonate, polyacrylate, polymethyl methacrylate, and silicone. Moreover, as a material of the outer introduction tube 23, the outer outlet tube 24, and the outer tip tube 25, fused silica or the like can be used. Further, examples of the material of the membrane 26 include resins such as cellulose acetate, regenerated cellulose, polyacrylonitrile, polycarbonate, polysulfone, polyether sulfone, and polymethyl methacrylate.

  FIG. 2B is a view showing a procedure for assembling the probe assembly 20. Each member is assembled in the direction of the arrow in this figure to assemble the probe assembly 20. That is, the proximal end side of the inner introduction tube 21 is inserted into the outer introduction tube 23, and the proximal end side of the inner extraction tube 22 is inserted into the outer extraction tube 24. The distal end side of the inner introduction tube 21 and the inner outlet tube 22 is inserted into the outer distal tube 25. A membrane 26 is attached to the lower end side of the outer distal tube 25.

  Next, the probe assembly 20 is assembled and fixed to the probe container 10. When assembling the probe assembly 20 to the probe holder 10, as shown in FIG. 3B, the outer introduction tube 23 is inserted into the introduction tube insertion groove 10b, the outer extraction tube 24 is inserted into the extraction tube insertion groove 10c, and the outer tip tube. 25 is fitted and fixed to the distal tube insertion groove 10d. Thereby, the probe main body 3 shown in FIG. 3B is completed.

(3) Guide cannula 30
As shown in FIG. 1, the guide cannula 30 has a substantially rectangular shape when viewed from the front, and has a portion whose diameter is reduced toward the lower end side. Inside the guide cannula 30, an accommodation space 30a having a shape corresponding to the outer surface of the probe container 10 is formed, and the upper surface 30b side of the guide cannula 30 is an open end opened to the outside. As a result, the probe container 10 can enter and be accommodated from the open end into the accommodation space 30a.

  The upper surface 30b of the guide cannula 30 has a rectangular shape in plan view, and both short sides thereof protrude in the width direction to form a pair of engaging protrusions 31. The engagement protrusion 31 is a member that can be engaged with an engagement claw 43 of a stopper described later. The side surface of each engagement protrusion 31 is formed with an inclined portion 31 a that expands in the width direction as it goes in the insertion direction of the container 10. In the present invention, the engagement protrusion 31 may have any shape as long as it extends in a direction intersecting the insertion direction B1-B2. Here, the “intersecting direction” means a direction that is not parallel to the insertion direction B1-B2, and a direction having a predetermined angle in addition to a direction (vertical direction) that is 90 degrees with respect to the insertion direction B1-B2. (Oblique direction) may be sufficient. In the present embodiment, the engagement protrusion 31 extends in the vertical direction (width direction) with respect to the insertion direction B1-B2.

  A first vertical surface 32 is formed below the engaging claw 43, and a tapered inclined surface 33 is formed below the first vertical surface 32. Further below that, a second vertical surface 34 substantially parallel to the first vertical surface 32 is formed, and below that, a constricted portion 35 reduced in the width direction is formed. Below the constricted portion 35, a flange portion 36 that is enlarged in the width direction is formed.

  Circular engagement holes 37 are formed in the front and back planes of the guide cannula 30, respectively. The engagement hole 37 has a size capable of being inserted through the raised portion 16 of the probe container 10. The engagement hole 37 engages with the raised portion 16 when the probe storage device 10 is advanced into the storage space 30a of the guide cannula 30 so that the probe storage device 10 is pulled out on the side opposite to the insertion direction. Can be prevented, and has a role of preventing slipping.

  The material of the guide cannula 30 is not particularly limited, and examples thereof include resins such as polyethylene, polytetrafluoroethylene, polypropylene, polycarbonate, polyacrylate, polymethyl methacrylate, and silicone.

(4) Stopper 40
The stopper 40 has a circular arc portion 41 having a shape (so-called “kamaboko-shaped”) in which an ellipse is divided into half in the major axis direction when viewed from above. On the side surface on the long diameter side of the arc portion 41 (the side surface opposite to the arc), a notch portion 41a recessed in a U-shape is formed. The notch 41a has substantially the same width as the engagement recess 12 of the probe container 10, and can be engaged by inserting the engagement recess 12 inward. Accordingly, the probe container 10 can be detachably attached by inserting the stopper 40 in a direction perpendicular to the insertion direction from the side surface of the probe container 10.

  On both sides of the cutout portion 41a of the arc portion 41, bent portions 42 that are bent downward are formed. The bent portion 42 has flexibility and can be elastically deformed in a direction crossing the insertion direction B1-B2. Here, the “intersecting direction” means a direction that is not parallel to the insertion direction B1-B2, and a direction having a predetermined angle in addition to a direction (vertical direction) that is 90 degrees with respect to the insertion direction B1-B2. (Oblique direction) may be sufficient. An engaging claw 43 that is bent inward in the width direction is formed on the distal end side of the bent portion 42. When the bent portion 42 is elastically deformed, the engaging claw 43 is displaced in a direction intersecting the insertion direction B1-B2. In addition, the front end side surface of the engaging claw 43 is formed with an inclined portion 43a inclined upward.

  The material of the stopper 40 is not particularly limited, and examples thereof include resins such as polyethylene, polytetrafluoroethylene, polypropylene, polycarbonate, polyacrylate, polymethyl methacrylate, and silicone.

  Next, a procedure for performing microdialysis using the microdialysis probe 1 will be described with reference to FIGS. In FIG. 5, the probe assembly 20 is omitted for easy understanding of the drawing. First, for a mouse (not shown) to be tested, a skull at the top of the head is drilled using a known device to form a hole leading to the brain. Then, the lower surface of the flange portion 36 of the guide cannula 30 is fixed to the head of the mouse, and the entire outer peripheral portion is covered with an adhesive material such as dental cement to the upper side of the inclined portion 33, and the lower side of the guide cannula 30 is cemented. Embed in. In this state, the cement is solidified, and the guide cannula 30 is fixed to the head of the mouse.

  After the cement is solidified, a dummy cannula (not shown) is inserted into the accommodation space 30a of the guide cannula 30. The dummy cannula is an instrument that does not include the probe assembly 20 and is inserted into the accommodation space 30a until microdialysis is performed. The dummy cannula has a role of closing the accommodation space 30a of the guide cannula 30 until the physical strength of the perforated mouse is recovered and microdialysis is performed. The dummy cannula is shaped like the probe container 10 but does not need to accommodate the probe assembly 20, and therefore the tube insertion grooves 10b, 10c, and 10d are not formed. Moreover, since it is necessary to extract from the guide cannula 30 when performing microdialysis, the raised portion 16 is not provided.

  When the mouse recovers and microdialysis is performed, first, the dummy cannula is extracted from the accommodation space 30a of the guide cannula 30. A stopper 40 is attached to the probe container 10. As shown in FIG. 4A, the stopper 40 is moved horizontally in the A1-A2 direction from the front surface of the probe container 10, and the notch 41a of the stopper 40 is fitted into the engagement recess 12 of the probe container 10. . The A1-A2 direction is perpendicular to the direction in which the engagement protrusion 11 protrudes when the probe container 10 is inserted into the guide cannula 30, and the insertion direction B1-B2 of the probe container 10 It is also perpendicular to the direction.

  Subsequently, in a state where the stopper 40 is attached to the probe container 10, as shown in FIG. 4B, the insertion direction (B1-B2) from the lower side of the probe container 10 into the accommodation space 30a of the guide cannula 30. Plug in. As shown in FIG. 5B, when the probe container 10 is pushed downward, the engagement protrusion 31 of the guide cannula 30 and the engagement claw 43 of the stopper 40 come into contact with each other. If the probe container 10 is continuously pushed downward in this state, the bent portion 42 intersects the insertion direction B1-B2 with the vicinity of the base end (connecting portion with the arc portion 41) of the bent portion 42 as a fulcrum (in the drawing). It is elastically deformed in the direction of the arrow, and the engaging claw 43 is displaced in the direction intersecting the insertion direction B1-B2 (the direction of the arrow in the figure).

  Here, each of the engagement protrusion 31 and the engagement claw 43 is formed with an inclined portion 31a and an inclined portion 43a that are inclined toward the distal end side. Since these inclined portions 31a and 43a are inclined in the same direction, when the stopper 40 is pushed downward, the engaging claw 43 slides between the inclined portion 31a and the inclined portion 43a so that the engaging claw 43 of the engaging protrusion 31 Enter the bottom. Thereby, the probe container 10 can be smoothly attached to the guide cannula 30.

  Further, once the engaging claw 43 enters the engaging protrusion 31 downward and is fixed, the engaging claw 43 is bent inward in the width direction, and the engaging claw 43 and the system protrusion 31 are reversed. Since it has a stop structure, it is possible to prevent the probe container 10 from coming off on the side opposite to the insertion direction. When the probe container 10 is inserted into the housing space 30a, the raised portion 16 of the probe container 10 protrudes into the engagement hole 37 of the guide cannula 30 and engages. This can also prevent the lobe fixture 10 from coming off in the direction opposite to the insertion direction.

  Thus, as shown in FIG. 4C, the microdialysis probe 1 in which the probe main body 3 is fixed to the guide cannula 30 is completed. In this state, an experiment by the microdialysis method is performed. For example, Ringer's solution or the like is continuously introduced to the membrane 26 side through the inner introduction tube 21, and the substance in the mouse brain is selectively permeated from the membrane 26 and led out from the inner lead-out tube 22 together with the introduced Ringer's solution. The After that, a constant-line analysis is performed by a known method. For example, the Ringer's solution containing the derived brain substance is fractionated by a fraction collector (not shown), and each fraction is measured by an analytical instrument such as HPLC, thereby quantifying the brain substance.

  When the experiment by microdialysis is completed, the probe container 10 is removed from the guide cannula 30. First, from the state of FIG. 4C, the stopper 40 is slid in the opposite direction (A2-A1 direction) when the stopper 40 is attached in FIG. Next, the probe container 10 is removed from the housing space 30a in the opposite direction (B2-B1 direction) to the time of insertion.

  Through the above procedure, the experiment of the microdialysis method using the microdialysis probe 1 is completed. The extracted probe container 10, probe assembly 20, stopper 40, and the like are discarded according to a predetermined rule. In the present embodiment, the probe body 3 can be removed and replaced with another probe body. As a result, it is possible to conduct experiments using various dialysis probes.

  Next, modified examples of the present invention will be described. In the above embodiment, the microdialysis probe 1 has been described as an example. However, the present invention is not limited to this, and can be used for, for example, embedding a sensor chip in the biosensor field.

  The engaging projection 41 of the guide cannula 30 and the engaging claw 43 of the stopper 40 can be reversed. That is, it is good also as an aspect which forms the engaging protrusion which protrudes in the direction which cross | intersects the insertion direction in a stopper, and forms the engaging claw which can be displaced to the guide cannula 30 in a guide cannula.

  The raised portion 16 of the probe container 10 and the engagement hole 37 of the guide cannula 30 can be reversed. In other words, a hemispherical projection may be formed on the inner wall surface on the front side of the accommodation space 30a of the guide cannula 30, and an engagement hole corresponding to this may be formed in the probe accommodation tool 10.

  In the above-described embodiment, the engagement protrusion 41 of the guide cannula 30 is configured to protrude in the width direction (that is, the direction perpendicular to the insertion direction B1-B2). However, the present invention is not limited to this, and the insertion direction May project in any direction as long as it intersects. For example, it may project obliquely downward with respect to the insertion direction, or may project obliquely upward. Similarly, the engaging claw 43 of the stopper 40 may be displaced in any direction as long as it intersects the insertion direction.

  In the above embodiment, the mouse is used as an experimental animal and the brain is taken as an example of a living organ. However, the experimental animal is not limited to this, and rats, guinea pigs, hamsters, rabbits, Xenopus, and dogs. It can be used for other laboratory animals such as pigs and monkeys. Moreover, it can be used not only for experimental animals but also for livestock such as cattle and horses and humans. Further, the organ into which the probe is inserted is not limited to the brain, and can be used for blood vessels, heart, lungs, skin, kidneys, liver, and the like.

DESCRIPTION OF SYMBOLS 1 Microdialysis probe (probe for biological insertion), 2 probe fixing tool, 3 probe main body, 10 probe container, 10a end surface, 10b introduction tube insertion groove, 10c lead-out tube insertion groove, 10d tip side tube insertion groove, 11 angle , 12 engaging recess, 13 vertical surface, 14 inclined surface, 15 tip convex portion, 16 raised portion, 20, probe assembly, 21 inner introduction tube, 22 inner outlet tube, 23 outer inlet tube, 24 outer outlet tube, 25 Outer tip tube, 26 membrane, 30 guide cannula, 30a receiving space, 30b upper surface, 31 engaging protrusion, 31a inclined portion (first inclined portion), 32 first vertical surface, 33 inclined surface, 34 second vertical surface, 35 Constriction part, 36 flange part, 37 engagement hole, 40 stopper, 41 Arc part, 41a Notch part, 42 Bent part, 43 Engagement claw, 43a Inclined part (second inclined part)

Claims (6)

A probe container capable of accommodating a probe assembly;
A guide cannula having a storage space inside which the probe storage tool can be inserted and stored;
A stopper attached to the probe container and capable of fixing the probe container accommodated in the accommodation space to the guide cannula,
Either one of the guide cannula and the stopper has an engagement protrusion that protrudes in a direction that intersects the insertion direction of the probe container into the storage space.
The other of the guide cannula and the stopper has an engaging claw that is displaceable in a direction crossing the insertion direction,
When the probe holder is inserted into the receiving space with the stopper attached to the probe holder, the engaging claw is displaced and engages with the engaging protrusion. Ingredients. The probe container is provided with an engagement recess formed in a side surface formed with a groove extending along a direction perpendicular to a direction in which the engagement protrusion projects.
The stopper has a notch having a shape corresponding to the engaging recess, and can be detachably attached to the probe holder by inserting the engaging recess into the notch in the vertical direction. The probe fixture according to claim 1, wherein: The engaging claw includes a bent portion that extends in the insertion direction and can be elastically deformed on the base end side,
3. The probe fixture according to claim 1, wherein the bent portion is elastically deformed in a direction intersecting the insertion direction to displace the engaging claw. 4. The engagement protrusion has a first inclined portion whose diameter is enlarged on the tip side in the insertion direction,
The probe fixture according to any one of claims 1 to 3, wherein the engaging claw has a second inclined portion whose tip side is reduced in diameter in the insertion direction. Either one of the probe container and the guide cannula includes a raised portion protruding from the surface,
The other of the probe container and the guide cannula is provided with an engagement hole formed at a position corresponding to the raised portion when the probe container is inserted into the housing space. The probe fixture of any one of -4.   A probe for insertion into a living body comprising the probe fixture according to any one of claims 1 to 5 and a probe assembly accommodated in the probe receptacle.

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