JP2015189742A - Ppar activity composition and energy production facility - Google Patents

Abstract

PROBLEM TO BE SOLVED: To discover new raw material for extraction of PPAR compositions other than gramineous plants, and thus obtain a PPAR composition easily available for effective use.SOLUTION: A PPAR activity composition is mainly composed of extract obtained by extracting Chlamydomonas fine algae with extraction solvent.

Description

  The present invention relates to a technique using a peroxisome proliferator-activated receptor (PPAR) active composition.

  PPAR is a ligand-dependent transcription factor belonging to the nuclear receptor superfamily, like steroid receptors, retinoid receptors and thyroid receptors, and so far three isoforms (α Type, δ (or β) type, and γ type) have been identified in various animal species including humans (see Non-Patent Document 1). Among these, PPARα is expressed in the liver, kidney, skeletal muscle and the like having high fatty acid catabolism, and is particularly highly expressed in the liver (see Non-Patent Document 2), and is related to fatty acid metabolism and intracellular transport. By positively or negatively controlling the expression of genes (eg, acyl CoA synthase, fatty acid binding protein, lipoprotein lipase, etc.) and apolipoprotein (AI, AII, CIII) genes related to cholesterol and neutral lipid metabolism Yes. PPARδ is ubiquitously expressed in each tissue in the body centering on nerve cells. At present, the physiological significance of PPARδ is unknown. PPARγ is highly expressed in adipocytes and is involved in adipocyte differentiation (see Non-Patent Document 3). Thus, each isoform of PPAR performs a specific function in a specific organ or tissue.

  It is known that such a PPAR active composition is contained in a grass family plant. Specifically, an extract of a PPAR active composition contained in a sorghum plant is described in Patent Document 1. Yes. However, in general, even if a plant belongs to the same family or a plant of a different genus produces the same type of active substance, the active substance has an activity equivalent to that of an active substance obtained from a plant of another genus. The activity may not be inhibited by some factor. Therefore, when searching for such active substances, it is often necessary to develop extraction methods and separation methods peculiar to each plant, and active substances cannot be easily developed.

  Therefore, the present inventors have previously found a PPAR composition derived from sugarcane for the purpose of providing a peroxisome proliferator-responsive receptor (PPAR) active composition that can be efficiently obtained by a simple technique. (Patent Document 2).

JP 2004-161656 A JP 2013-193987 A

Proceedings of the National Academy of Sciences, 1992, 89, 4653-4657. Endocrinology, 1996, 137, 354-366 Journal of Lipid Research, 1996, 37, 907-925.

  However, as in the configuration of Patent Document 2, the PPAR composition contains an inhibitor and the activity of the PPAR composition may be suppressed. The activity of the PPAR composition is reduced by reducing the inhibitor or the like. It may be required to improve That is, when a known PPAR composition is used, the extracted PPAR composition cannot be used as it is, and a highly active PPAR composition can be effectively used only with other accompanying operations. Is the current situation. Therefore, it is necessary to search for a PPAR composition raw material that can obtain a PPAR composition having high activity more easily.

  In addition, when a PPAR composition is extracted from a plant, the extracted plant is discarded, but the plant waste in a state containing the extraction solvent cannot be discarded as it is, and it is effectively used for other purposes. It is requested to do.

  Therefore, an object of the present invention is to find a raw material for extracting a new PPAR composition in addition to a grass family plant, to obtain a PPAR composition that is easy to use effectively, and to further efficiently extract a PPAR composition. It also has the point of obtaining energy production facilities.

  As a result of intensive studies, the present inventors have clarified that an extract obtained by extracting Chlamydomonas microalgae with an extraction solvent contains a PPAR active substance.

[Configuration 1]
Then, the characteristic structure of this invention for solving the said subject exists in the point which has as a main component the extract which extracted the microalga of Chlamydomonas genus with the extraction solvent.

[Function 1]
From the above-mentioned new findings, it has become clear that Chlamydomonas microalgae can be used as a PPAR composition raw material other than Gramineae plants. And since the extract by the extraction solvent can be obtained as a PPAR composition, the form which can utilize a PPAR composition effectively could be provided.

  It is known that the PPAR active substance extracted in this configuration includes any of PPARα, PPARβ, and PPARγ. Even if the physiological activity of PPARβ is not known at present, these substances are considered to function effectively as pharmaceuticals and functional food ingredients and are highly useful.

  The PPAR active substance referred to in the present invention is a substance containing a compound having the ability (activity) to bind to the ligand binding region of PPAR, and the PPAR active composition contains such a substance as a component, It refers to a composition having the above activity. PPAR ligand activity can be determined by, for example, a reporter assay (Cell, 1995, 83, 803-812) for evaluating the binding of a PPAR ligand binding region to a GAL4 fusion protein by expression of luciferase, It can be measured by a competition binding assay (Cell, 1995, 83, 813-819) or the like using a protein containing the protein. In these assays, the activity of the sample is generally compared to the solvent control, and a sample that exhibits higher activity than the solvent control and is found to be dose-dependent is evaluated as “with PPAR activity”.

  The extraction method is not particularly limited, and various extraction operations such as solvent extraction, steam distillation, and extraction with carbon dioxide using a supercritical extraction technique can be used. Furthermore, the extract can be purified and used, but as long as it does not contain impurities that are inappropriate for food and drink or pharmaceuticals, it is used as an extract or in the form of a crude extract or semi-purified extract. it can.

  The microalgae used in the present invention is an algae containing microscopic phytoplankton that floats in water and grows by photosynthesis, and refers to those that form single cells or colonies with eubacteria, cyanobacteria and eukaryotes. For example, Euglena algae, which are microalgae that perform photosynthesis and that do not grow on the inner wall surface of the aeration tank, can be mentioned. Euglena algae is a group of flagellates and is included in the group of unicellular eukaryotic algae including Euglena and the like as a motile algae. As described above, Euglena algae is a motile algae and does not grow on the inner wall surface of the aeration tank or the channel wall surface of the treated sewage. Therefore, there is no blockage of the flow path of the treated sewage due to the growth, and when photosynthesis is performed, nitrogen and phosphorus are introduced and propagated in the eutrophic environment in the treated sewage, so that the treated sewage is purified. The

[Configuration 2]
In the above configuration, the microalgae may be Chlamydomonas reinhardtii.

[Operation effect 2]
When Chlamydomonas line hardy is used as a microalgae, wastewater handled by general sewage treatment facilities can be grown as a nutrient source, and as it grows, hydrogen can be produced and further energy can be supplied. This is preferable. In addition, since microalgae can be handled in a liquid state, it is easy to handle. In particular, the use of Chlamydomonas line hardy that can take the above growth form is advantageous in that it is easy to adopt a form in which a PPAR composition is continuously supplied. It is.

[Configuration 3]
The extraction solvent can be an ethanol-containing solvent or an acetone-containing solvent.

[Operation effect 3]
As the extraction solvent, various solvents such as ethanol, acetone, chloroform, methylene chloride, and ethyl acetate can be used from the viewpoint of extraction efficiency. However, the extraction solvent hardly affects the growth environment of microalgae and has a PPAR composition. In consideration of the convenience when discarding the microalgae waste liquid after the extraction of the product, it is preferable to use an ethanol-containing solvent or an acetone-containing solvent that hardly affects the environment.

[Configuration 4]
Moreover, it is preferable that the said extract is the thing which contact-treated the carbon adsorbent after extraction with an extraction solvent.

[Operation effect 4]
In many cases, so-called inhibitors are contained in the PPAR composition, which contributes to the inability to fully utilize the activity of the PPAR composition. Therefore, when the PPAR composition is contact-treated with the carbon adsorbent, it is considered that the carbon adsorbent does not adsorb a substance having a high PPAR activity in the PPAR composition but preferentially adsorbs an inhibitor. Inhibitors can be selectively removed. Therefore, the PPAR composition can be made highly active.

[Configuration 5]
The characteristic configuration of the energy production facility of the present invention is as follows:
Providing a microalgae culture tank containing Chlamydomonas microalgae to produce hydrogen by irradiating the microalgae in the microalgae culture tank with carbon dioxide-containing gas and irradiating with light while supplying nutrients In addition, the microalgae are proliferated, the proliferated microalgae are taken out of the microalgae culture tank, and a microalgae extraction tank is provided that extracts the extracted microalgae with an extraction solvent to obtain a PPAR active composition. It is in the point which provided the methane fermenter which obtains biogas by methane fermentation of the generated extraction residue.

[Operation effect 5]
According to the said structure, not only can a micro algae be cultured with a micro algae culture tank, but hydrogen can be produced and supplied as an energy source. In this state, since the microalgae in the microalgae culture tank grow, the proliferated microalgae can be taken out and used effectively. In the microalgae extraction tank, the PPAR composition can be obtained by extracting the extracted microalgae with an extraction solvent. In this extraction operation, the culture solution in the microalgae culture tank is used as it is, and it can be brought into contact with the extraction solvent while solid-liquid separation by natural filtration in the microalgae extraction tank. You may have a means to do. Moreover, since the extraction residual after recovering the PPAR composition is biomass, it can be used for methane fermentation, where methane fermentation can be performed and methane gas can be supplied as an energy source.

  That is, according to the above configuration, hydrogen gas can be supplied as energy during the cultivation process of microalgae, and methane gas can be supplied as energy after the recovery of the PPAR composition. A good energy supply.

[Configuration 6]
In addition, in the said structure, the gas containing the said carbon dioxide gas can be made into the waste gas from a cogeneration apparatus, and the said nutrient source can be made into the sewage of a sewage treatment facility.

[Operation effect 6]
In the above configuration, the microalgae culture tank requires a nutrient source, carbon dioxide gas, and light for microalgae culture. As a result, microalgae photosynthesize and produce hydrogen, but in the state where sunlight enters the wastewater as a nutrient source and the exhaust gas is introduced, like an aeration tank in a sewage treatment facility, Microalgae that perform photosynthesis can be cultured in sewage using an aeration tank as a microorganism culture tank. Therefore, microalgae can perform photosynthesis using the treated sewage, carbon dioxide in the exhaust gas, and sunlight in the treated sewage in the aeration tank, thereby fixing the carbon dioxide. .
In addition, since the aeration tank of a sewage treatment facility usually has a volume of several thousand cubic meters or more, a large amount of microalgae can be cultured and propagated in the treated wastewater, so that more carbon dioxide gas Can be immobilized. Further, since the microalgae absorb and grow nutrients such as nitrogen and phosphorus contained in the treated sewage, purification of the treated sewage is promoted.

  Here, for example, when the cogeneration apparatus is adjacent to the energy production facility, the exhaust gas from the cogeneration apparatus can be used as the exhaust gas supplied to the microorganism culture tank. Then, carbon dioxide gas can be stably supplied from the cogeneration apparatus. Further, carbon dioxide in the exhaust gas is fixed as microalgae without being released into the atmosphere. Moreover, since the methane gas collect | recovered with a fermenter can be supplied to a cogeneration apparatus, it can be set as the rational structure with which energy production and consumption are achieved efficiently.

  Therefore, microalgae can be used as an alternative raw material for sugarcane waste, and PPAR active ingredients can be obtained efficiently, and can be used as health foods, beverages and pharmaceuticals for improving metabolic syndrome.

Graph showing PPARα activity of each specimen Graph showing PPARδ activity of each specimen Graph showing PPARγ activity of each specimen Schematic diagram of energy production equipment

  Below, the PPAR active composition of this invention is demonstrated. In addition, although suitable examples are described below, these examples are described in order to more specifically illustrate the present invention, and various modifications can be made without departing from the spirit of the present invention. The present invention is not limited to the following description.

[Extraction of PPAR active composition]
The culture solution of the microalga Chlamydomonas reinhardtii Dangerard (NIES-2235) was centrifuged and the sediment was collected to obtain a microalgae sample.

  The microalgae sample was extracted with water, ethanol, and acetone.

(1) Extraction with water 9.75 g (wet weight) of a microalgae sample was suspended in 11 ml of NaCl water (0.9 w / v%, sterilized). PPAR composition in microalgae samples by sonication (using Vibra Cell, Sonics & Material Inc., Amplitude: 40, Pulser: 3 minutes x 3 times, the same sonication below) Was eluted. This was subjected to solid-liquid separation by centrifugation (39,800 × g, 20 min., 4 ° C., the same conditions for the following centrifugation) to obtain 15 mL of the supernatant (the above NaCl water was used during the recovery operation to obtain a final concentration of 0). (The above NaCl water was used as appropriate in the following operation).

The supernatant was filtered and concentrated (about 0.2 mL) using an ultrafiltration membrane (Amicon Ultra 30K), and concentrated to dryness to give Sample 1.
The filtrate obtained with Sample 1 was filtered and concentrated using an ultrafiltration membrane (Amicon Ultra 3K) (about 0.8 mL), and concentrated to dryness to give Sample 2.
The filtrate (10 mL) obtained together with sample 2 was concentrated to dryness to obtain sample 3.

(2) Ethanol extraction 9.44 g of a microalgae sample was suspended in 99.5% ethanol, 13.75 ml. This was sonicated to elute the PPAR composition in the microalgae sample. This was subjected to solid-liquid separation by centrifugation to obtain 19.5 mL of the supernatant (the above ethanol was used during the collection operation to a final concentration of 75 v / v%. The above ethanol was also used in the following operations as appropriate).

The supernatant obtained by concentrating to dryness was redissolved in 2 ml of 75 v / v% ethanol. 1 ml of this was sampled and concentrated to dryness with an evaporator to give sample 4.
Further, the remaining 1 ml was desorbed by an open column packed with 0.75 g of graphite carbon (5982-4482, SampliQ Carbon SPE, Agilent Technologies), and the resulting solution was concentrated and dried with an evaporator. -2.

(3) Acetone extraction 9.08 g of a microalgae sample was suspended in 11.5 ml of 99.9% or more pure acetone. This was sonicated to elute the PPAR composition in the microalgae sample. This was subjected to solid-liquid separation by centrifugation to obtain 18 mL of the supernatant (the above acetone was used during the recovery operation to a final concentration of 70 v / v%. In the following operation, the above acetone was also used as appropriate).

The supernatant obtained by concentrating to dryness was redissolved in 2 mL of 70% acetone. 1 ml of this was sampled and concentrated to dryness with an evaporator to give sample 5.
Further, the remaining 1 ml was desorbed by an open column packed with 0.75 g of graphite carbon, and the resulting solution was concentrated to dryness with an evaporator to obtain sample 5-2.

[Measurement of PPAR activity]
A fixed amount of DMSO was added to each of Samples 1-2 and gently stirred, and allowed to stand at room temperature for several hours. Subsequently, the sample was centrifuged (GILSON, GmCLab), and the supernatant was collected to be used as an evaluation sample. This sample for evaluation was adjusted so that the final concentration per assay system would be 1/3000, 1/1000, 1/300, 1/40 times, and a total of 28 specimens were prepared. The PPAR activity of these specimens was measured using EnBio RCAS for PPARα, EnBio RCAS for PPARδ, and EnBio RCAS for PPARγ (all manufactured by Fujikura Kasei Co., Ltd.), which are nuclear receptor / cofactor assay systems. As a result, the PPAR activity of each specimen was as shown in FIGS.

  1 to 3, it can be seen that each specimen contains a PPARα active substance and also contains PPARδ active substance and PPARγ to some extent. It can also be seen that the PPAR composition can be extracted using any of water, ethanol, and acetone. From FIG. 1, PPARα is contained in a large amount in a low molecular weight region of less than 3K, but activity was also observed in a region of 30K or more.

  In addition, when samples 4 and 4-2, 5 and 5-2 are compared, the PPAR activity is improved by treatment with a graphite carbon column (particularly in high-concentration specimens), and activity inhibitors Was removed by a graphite carbon column and was found to be effective to obtain a more active PPAR composition.

[Energy production equipment]
Hereinafter, the energy production equipment concerning the present invention is explained based on a drawing. A sewage treatment facility 1 as an energy production facility according to the embodiment of the present invention shown in FIG. 4 includes a cogeneration device 2 that discharges an exhaust gas E containing carbon dioxide gas, and a sand basin 6 into which treated sewage W flows from a sewer pipe. And an aeration tank 3 for aeration of the treated sewage W and a first settling tank 4 and a second settling tank 5 for collecting sludge in the treated sewage W.
As shown in FIG. 4, the treated sewage W flowing into the sewage treatment facility 1 from the sewer pipe is stored in the sand basin 6, the first sedimentation tank 4, the aeration tank 3 as a microalgae culture tank, and the second sedimentation tank 5. It flows in order, and the treated waste water W of the supernatant of the second sedimentation tank 5 flows out into a river or the like after chlorine disinfection as treated water that has been treated. In addition, a microalgae extraction tank 51 is connected to the second sedimentation tank 5.

  In the sand basin 6, large garbage and earth and sand G contained in the treated wastewater W are deposited. Then, the treated sewage W exiting the sand basin 6 flows into the first sedimentation tank 4. In the 1st sedimentation tank 4, 1st sedimentation sludge S1 which is comparatively easy to sink is settled. Thereafter, the treated sewage W flows into the aeration tank 3. In the aeration tank 3, aerobic microorganisms called activated sludge are mixed into the treated sewage W by aeration to oxidatively decompose dissolved / floating organic substances in the treated sewage W. Further, the microalgae 8 is introduced into the treated wastewater W, and the microalgae 8 performs photosynthesis so that the carbon dioxide gas supplied to the aeration tank 3 is fixed to the microalgae 8.

  Thereafter, the treated sewage W flows into the second settling tank 5 and the activated sludge S2 (corresponding to sludge) containing the microalgae 8 is precipitated in the second settling tank 5 to complete the treatment of the treated sewage W as a supernatant. The treated water is discharged into rivers after chlorination. On the other hand, a part of the activated sludge S2 precipitated here is returned to the aeration tank 3 and used for oxidative decomposition of dissolved / floating organic substances in the treated wastewater W.

The aeration tank 3 will be described. The aeration tank 3 into which the treated sewage W flows includes solar light incident means for allowing sunlight T to enter the treated sewage W and exhaust gas input means for introducing exhaust gas E. Is configured so that the microalgae 8 for photosynthesis can be cultured in the input state where the sunlight T enters the treated wastewater W of the aeration tank 3 and the exhaust gas E is input to the aeration tank 3 by the exhaust gas input means. ing.
In this embodiment, since the upper part of the aeration tank 3 is made into an open state, and it is set as the structure which makes sunlight T enter the process wastewater W, this upper opening 3a comprises the sunlight incident means.

Further, the aeration tank 3 is provided with a diffuser 7 for spraying air A into the treated sewage W in order to keep the treated sewage W in an aerobic state and oxidize and decompose organic matter in the treated sewage W with activated sludge. ing. The diffuser 7 is provided on the entire inner bottom portion of the aeration tank 3, and is a full-aeration type in which the air A supplied from the gas supply pipe 15 a is dispersed over the entire area of the treated sewage W in the aeration tank 3.
And since the upper part of the aeration tank 3 is made into the open state in order to discharge the air A spread | diffused by the diffuser 7 from the aeration tank 3, by using the upper opening 3a also as a sunlight incident means, Sunlight T can enter the aeration tank 3. Usually, since the aeration tank 3 of the sewage treatment facility 1 has a volume of several thousand cubic meters or more, a large amount of microalgae 8 can be cultured and propagated in the treated wastewater W. Carbon dioxide can be fixed.

  Further, by supplying the exhaust gas E of the cogeneration apparatus 2 supplied to the gas supply pipe 15a by the exhaust gas supply pipe 15b in addition to the air A to the gas supply pipe 15a connected to the diffuser 7, the diffuser 7 Can be used as exhaust gas charging means, and the diffuser 7 can simultaneously spray the air A and the exhaust gas E into the treated wastewater W. In this way, by mixing the high temperature exhaust gas E with the low temperature air A, the exhaust gas E having a lowered temperature can be supplied into the treated wastewater W, and the microalgae 8 in the treated wastewater W to be described later can be supplied. Carbon dioxide gas can be supplied without adversely affecting high temperatures. Therefore, the microalgae 8 can be cultured by supplying the carbon dioxide gas of the exhaust gas E to the microalgae 8 using the existing equipment in the sewage treatment facility 1.

  In this way, the sunlight T is incident on the treated wastewater W from the upper opening 3a of the aeration tank 3, and the exhaust gas E is sprayed on the treated wastewater W by the diffuser 7. The microalgae 8 introduced into the can be subjected to photosynthesis using the treated sewage W, carbon dioxide in the exhaust gas E, and sunlight T. Thereby, carbon dioxide can be fixed by the microalgae 8. Furthermore, since the microalgae 8 is cultured and proliferated in a state of absorbing nitrogen and phosphorus, which are nutrients contained in the treated sewage W, the treated sewage W is purified and more carbonic acid is produced by the proliferated microalgae 8. Gas can be immobilized.

Next, the microalgae 8 will be described. The microalgae 8 used in the present invention is a microalgae 8 that performs photosynthesis and that does not grow on the inner wall surface of the aeration tank 3, and examples thereof include Euglena algae. Euglena algae is a group of flagellates and is a group of unicellular eukaryotic algae including Euglena as a motile algae. Euglena algae are motility algae, so they do not adhere to the inner wall surface of the aeration tank 3 or the channel wall surface of the treated sewage W. Therefore, the blockage of the flow path of the treated wastewater W does not occur, and the function of the sewage treatment facility 1 is not hindered. Moreover, since this Euglena algae takes in nitrogen and phosphorus and proliferates in the eutrophic environment in the treated sewage W with performing photosynthesis, the treated sewage W is purified.
On the other hand, for example, in seaweeds represented by aonori and seaweed, the root-like part sticks to rocks and the like, and the leaf-like part extends into the water. Especially in brown algae such as seaweed, a large species exceeding 1 m. Therefore, since it adheres to the flow path wall surface of the treated wastewater W and may cause a blockage of the flow path of the treated wastewater W, it is suitable as the microalgae 8 used in the present invention. is not.

  The 2nd sedimentation tank 5 is demonstrated. The second settling tank 5 is located on the downstream side of the flow of the treated wastewater W in the aeration tank 3. The treated sewage W in which the microalgae 8 and the activated sludge are mixed flows out from the aeration tank 3 and flows into the second settling tank 5. Then, activated sludge S2 (corresponding to sludge) containing microalgae 8 is deposited at the bottom. A sludge transport pipe 10a and a sludge return pipe 10b are connected to the bottom of the second sedimentation tank 5, and a part of the precipitated activated sludge S2 is returned to the aeration tank 3 by the sludge return pipe 10b as described above. , Mixed in the treated waste water W and used again.

  A part of the activated sludge S2 containing the microalgae 8 is dehydrated by the dehydrator 16 equipped with a solid-liquid separation membrane and then transported to the microalgae extraction tank 51. The remaining activated sludge S2 is transported to the methane fermentation apparatus 9 by the sludge transport pipe 10a.

  The microalgae extraction tank 51 includes an extraction solvent supply unit 51a, and adds an extraction solvent Sol such as ethanol to the activated sludge S2 containing the dehydrated microalgae 8 to extract the PPAR composition. The extracted extract is recovered as a PPAR composition P by the membrane separation device 51b. The extraction residual S4 subjected to membrane separation is supplied to the methane fermentation apparatus 9 from the drawing path 51c.

The methane fermentation apparatus 9 will be described. In the sewage treatment facility 1 according to the present invention, the first sedimentation sludge S1 collected in the first sedimentation tank 4, the activated sludge S2 containing the microalgae 8 collected in the second sedimentation tank 5, and the microalgae extraction A methane fermentation apparatus 9 for methane fermentation of mixed sludge S3, which is a mixture of extraction residuals S4 from the tank 51, is provided. The methane fermentation apparatus 9 performs methane fermentation of the mixed sludge S3 containing the microalgae 8 to generate biogas fuel B mainly composed of methane. Therefore, since the mixed sludge S3 contains the microalgae 8 cultured and grown in the aeration tank 3, more biogas fuel B can be generated by the mixed sludge S3.
Moreover, in order to accelerate methane fermentation to the methane fermentation apparatus 9, the mixed sludge S3 is normally heated to about 35-40 degreeC in the methane fermentation apparatus 9. FIG. And the heat exchanger 9a in which the exhaust gas E from the cogeneration apparatus 2 mentioned later distribute | circulates is provided in this methane fermentation apparatus 9, and the mixed sludge S3 in the methane fermentation apparatus 9 is heated.

Further, a fuel supply pipe 11 for supplying the biogas fuel B generated by the methane fermentation apparatus 9 to the cogeneration apparatus 2 is provided. Thereby, the biogas fuel B produced | generated with the methane fermentation apparatus 9 is supplied to the cogeneration apparatus 2. FIG.
Therefore, the biogas fuel B generated in the methane fermentation apparatus 9 provided in the sewage treatment facility 1 can be consumed in the cogeneration apparatus 2 provided in the sewage treatment facility 1 without being transported to another place. it can. Thereby, the cost for transporting the biogas fuel B can be reduced. Moreover, the energy supplied to the sewage treatment facility 1 from the outside newly can be reduced by making the fuel of the cogeneration apparatus 2 into the biogas fuel B produced | generated with the methane fermentation apparatus 9. FIG.

A gas pretreatment device 12, a fuel tank 13, and a gas mixing device 14 are connected to the fuel supply pipe 11. Here, the gas pretreatment device 12 is provided with a desulfurization device 12a, a dehumidifier 12b, and a siloxane removal device 12c, which are configured to remove impurity components in the biogas fuel B.
The biogas fuel B produced by the methane fermentation apparatus 9 is introduced into the desulfurization apparatus 12a through the fuel supply pipe 11, and the hydrogen sulfide is processed. Next, since a large amount of water contained in the biogas fuel B adversely affects the adsorption and removal of siloxane in the siloxane removal device 12c, the biogas fuel B is introduced into the dehumidifier 12b and dehumidified. Then, it flows into the siloxane removal apparatus 12c and siloxane is removed. Thus, the biogas fuel B from which hydrogen sulfide and siloxane have been removed is stored in the fuel tank 13. The biogas fuel B stored in the fuel tank 13 is supplied to the gas mixing device 14 as necessary in accordance with the operation status of the cogeneration device 2.

  The cogeneration apparatus 2 to which the biogas fuel B stored in the fuel tank 13 is supplied includes a gas engine 2a and a generator 2b driven by the gas engine 2a. A mixed gas fuel M obtained by mixing the biogas fuel B and the city gas F is supplied to the gas engine 2a of the cogeneration apparatus 2. The mixed gas fuel M is generated by mixing the biogas fuel B and the city gas F in the gas mixing device 14.

  The power V generated by the generator 2b is configured to be supplied to each power load facility in the sewage treatment facility 1. Moreover, the heat exchanger 9a which comprises the methane fermentation apparatus 9 so that heating is possible by the exhaust heat of the gas engine 2a is provided in the methane fermentation apparatus 9. The heat exchanger 9a is configured so that heat is exchanged between the exhaust gas E of the gas engine 2a and the mixed sludge S3 in the methane fermentation apparatus 9. Thereby, using the exhaust heat of the exhaust gas E of the gas engine 2a, the mixed sludge S3 in the methane fermentation apparatus 9 can be heated to promote methane fermentation. Moreover, the electric power required for heating of the methane fermentation apparatus 9 can be reduced, and the heat recovery efficiency of the cogeneration apparatus 2 can be improved.

  Accordingly, microalgae can be used as an alternative raw material for sugarcane waste, and PPAR active ingredients can be obtained efficiently. For example, it can be used as health foods, beverages and pharmaceuticals for improving metabolic syndrome.

1 Sewage treatment facility (energy production equipment)
2 Cogeneration system 2a Gas engine 3 Aeration tank (microalgae culture tank)
3a Upper opening (sunlight entry means)
5 Second sedimentation tank (sludge collection tank)
51 Microalgae extraction tank 7 Diffuser (exhaust gas input means)
8 Microalgae 9 Methane fermentation equipment (methane fermentation tank)
11 Fuel supply pipe (fuel supply means)
B Biogas fuel E Exhaust gas M Mixed gas fuel S2 Activated sludge (sludge)
T Sunlight W Treated sewage

Claims (6)

  A PPAR active composition mainly comprising an extract obtained by extracting Chlamydomonas microalgae with an extraction solvent.   The PPAR active composition according to claim 1, wherein the microalga is Chlamydomonas reinhardtii.   The PPAR active composition according to claim 1 or 2, wherein the extraction solvent is an ethanol-containing solvent or an acetone-containing solvent.   The PPAR active composition according to any one of claims 1 to 3, wherein the extract is obtained by subjecting a carbon adsorbent to contact treatment after extraction with an extraction solvent.   Providing a microalgae culture tank containing Chlamydomonas microalgae to produce hydrogen by irradiating the microalgae in the microalgae culture tank with carbon dioxide-containing gas and irradiating with light while supplying nutrients In addition, the microalgae are proliferated, the proliferated microalgae are taken out of the microalgae culture tank, and a microalgae extraction tank is provided that extracts the extracted microalgae with an extraction solvent to obtain a PPAR active composition. An energy production facility equipped with a methane fermentation tank that obtains biogas by methane fermentation of the generated extraction residue.   An energy production facility in which the gas containing carbon dioxide is exhaust gas from a cogeneration device, and the nutrient source is sewage from a sewage treatment facility.

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