JP2015187612A - Aging evaluation device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To simply evaluate aging at a spot without using gene analysis and the like.SOLUTION: An aging evaluation device of the invention evaluates degree of progress of aging on the basis of a fluorescent substance which increases in a body accompanying the aging of organisms.

Description

  The present invention relates to an aging evaluation apparatus for evaluating human aging.

  Techniques for evaluating aging are conventionally known. For example, Patent Document 1 discloses an E-cadherin gene, T-cadherin (H-cadherin) gene, Polyvirus receptor gene, Integrin beta-1 gene, Laminin alpha 3 gene, Jagged 1 gene in a biological sample collected from a subject. Measuring the expression of at least one gene selected from the group consisting of Delta-like 1 gene and Keratin 10 gene, and evaluating the skin aging degree in the subject based on the expression level of the gene to be measured; A skin evaluation method is described.

JP 2010-115131 A (published May 27, 2010)

  However, the conventional techniques as described above are complicated, such as gene analysis, and require pretreatment such as sample preparation before performing gene analysis. Moreover, expensive analysis equipment is required for gene analysis. For this reason, it is impossible to measure and evaluate the degree of aging on the spot.

  The present invention has been made to solve the above problems, and an object of the present invention is to provide an aging evaluation apparatus that can easily evaluate aging on the spot without using genetic analysis or the like. It is.

  In order to solve the above problems, an aging evaluation apparatus according to one aspect of the present invention is an aging evaluation apparatus that evaluates the progress of aging based on a fluorescent substance that increases in the body with the aging of a living organism, A fingertip insertion part for inserting a fingertip, a measurement table for arranging the inserted fingertip, a light source part for irradiating excitation light, and fluorescence emitted by irradiating the fingertip with the excitation light A detecting unit for detecting; an incident optical fiber for propagating the excitation light emitted from the light source unit; and an outgoing optical fiber for receiving the fluorescence and connecting to the detecting unit, the incident optical fiber and the It is provided coaxially with the outgoing optical fiber, the measurement table has a hole at a predetermined position, and the incident optical fiber and the outgoing optical fiber are arranged toward the hole.

  According to one embodiment of the present invention, there is an effect that aging can be easily evaluated.

It is a figure which shows an example of a structure of the aging evaluation apparatus in this embodiment. It is the figure which showed the relationship between the wavelength of excitation light when a aging model organism is irradiated with excitation light, and a fluorescence spectrum. It is a graph which shows the increase in the fluorescence intensity accompanying the aging of an aging model organism. It is a figure which shows the structural-analysis result of 17 days old. It is a figure which shows the fluorescence spectrum obtained by saccharifying the protein which increases with aging. It is a figure which shows the fluorescence spectrum acquired when different food was given to each of two aging model organisms of 7 days old. It is a figure which shows an example of a structure of the aging evaluation apparatus in the modification 1. It is a figure which shows an example of a structure of a fingertip measuring apparatus. It is a figure which shows an example of a structure of the aging evaluation apparatus in the modification 3. It is a figure which shows the measurement probe with which the aging evaluation apparatus in the modification 4 is provided. It is a block diagram which shows an example of a structure of a control apparatus.

  Embodiments of the present invention will be described with reference to the drawings. In the following description, the same members are denoted by the same reference numerals. Their names and functions are also the same. Therefore, detailed description thereof will not be repeated.

  FIG. 1 is a diagram illustrating an example of a configuration of an aging evaluation apparatus in the present embodiment. As shown in FIG. 1, an aging evaluation apparatus 100 is an apparatus that evaluates human aging, and includes an excitation light source 101 (light source unit), a detector 103 (detection unit), a measurement probe 105, and a control device. 1.

  In the measurement probe 105, two types of optical fiber for incident 105a and optical fiber for outgoing 105b are provided coaxially. An excitation light source 101 is attached to one end of the incident optical fiber 105a, and the excitation light emitted from the excitation light source 101 is propagated and emitted to the other end of the incident optical fiber 105a. The excitation light source 101 is a light source that emits excitation light having a wavelength of 325 nm, and may be, for example, a tube type such as a halogen or xenon light source, an LED (manufactured by DOWA Electronics Co., Ltd.), or an LD.

  The end portion on the exit side of the incident optical fiber 105a is directed to, for example, a biological evaluation target. Thereby, the excitation light which the light source 101 for excitation emits to evaluation object can be irradiated. The applicant of the present application has focused on the fact that the accumulated amount of the fluorescent substance that fluoresces by the excitation light in the body increases as the living organism ages. A fluorescent substance is a substance that emits fluorescence when excited by light of a specific wavelength. Light that excites fluorescence is referred to as excitation light.

  The emission optical fiber 105b has an emission-side end portion of the incident optical fiber as an incident-side end portion, and fluorescence emitted from the evaluation target is incident from the incident-side end portion. The end of the emission side of the emission optical fiber 105 b is connected to the detector 103.

  The detector 103 is a device that detects fluorescence. For example, a CCD detector ILX511B (manufactured by SONY), a photodetector; PD (manufactured by Si PIN photodiode Hama Photo) CMOS image sensor, photomultiplier tube (PMT), channel Such as a TRON detector. The detector 103 detects the fluorescence propagating through the outgoing optical fiber 105 b and outputs the result to the control device 1.

  The control device 1 may be any device that can perform brightness adjustment of the excitation light source 101, irradiation / non-irradiation switching control, data storage, data display, and data analysis, for example, a personal computer. The control device 1 displays the fluorescence spectrum on the monitor as the detection result input from the detector 103.

  FIG. 11 is a block diagram illustrating an example of the configuration of the control device. As shown in FIG. 11, the control device 1 includes a control unit 300, an operation unit 310, a display unit 320, and a storage unit 330. Note that the control device 1 is, for example, a personal computer, and its hardware configuration is well known, so detailed description thereof will not be repeated here. The control unit 300 included in the control device 1 includes an aging evaluation value calculation unit (aging evaluation value calculation unit) 311 and an aging determination unit (aging determination unit) 313.

  The aging evaluation value calculation unit 311 calculates an aging evaluation value indicating the degree of progress of aging, and outputs the calculated aging evaluation value to the aging determination unit 313. Specifically, based on the measured fluorescence intensity and the reference fluorescence intensity stored in the storage unit 330, a value indicating the degree of the reference fluorescence intensity with respect to the measured fluorescence intensity is calculated as an aging evaluation value. The measured fluorescence intensity is the fluorescence intensity input from the detector 103. The aging evaluation value may be a difference or a ratio of the measured fluorescence intensity with respect to the reference fluorescence intensity.

  The aging determination unit 313 determines the progress of aging based on the aging evaluation value calculated by the aging evaluation value calculation unit 311 and the aging evaluation data stored in the storage unit 330, and controls the display unit 320, The determination result is displayed on the display unit 320. The aging evaluation data is data in which the degree of aging progress is defined by an aging evaluation value, and the degree of aging progress is associated with each of a plurality of aging evaluation value ranges.

  The aging determination unit 313 refers to the aging evaluation data stored in the storage unit 330, and includes a range including the aging evaluation value calculated by the aging evaluation value calculation unit 311 among a plurality of ranges indicated by the aging evaluation data. Identify. The aging determination unit 313 outputs the progress of aging corresponding to the range specified in the aging evaluation data to the display unit 320 as a determination result.

<Aging evaluation method>
Here, an aging evaluation method using the aging evaluation apparatus 100 will be described. In preparation for evaluation of aging, a fluorescence spectrum serving as an aging marker was identified. Specifically, using a commercially available (Hitachi fluorescence spectrophotometer FL-4500), the light emitted from the excitation light source 101 is adjusted in the range of 250 to 400 nm and irradiated to the aging and young age of the aging model organism. . Thereby, the characteristic fluorescence spectrum was able to be confirmed especially in the old age of the aging model organism. In addition, it is generally known that the aging model organism used is a nematode, and the nematode can be applied to a human aging model.

  FIG. 2 is a diagram showing the relationship between the wavelength of the excitation light and the fluorescence spectrum when the aging model organism is irradiated with the excitation light. The vertical axis represents the wavelength of excitation light from 250 to 400 nm, and the horizontal axis represents the fluorescence spectrum. In FIG. 2, the fluorescence spectrum corresponding to the wavelength of the excitation light is plotted. In FIG. 2, the bird's eye view having a high density shows a spectrum that emits fluorescence according to the wavelength of the specific excitation light. From FIG. 2, when excited with excitation light (Ex) having a wavelength of 325 nm, a fluorescence spectrum of 420 nm is obtained. It was confirmed that (Em) was present. For this reason, it is assumed that a fluorescent substance that emits Em: 420 nm by being excited at Ex: 325 nm is a substance that accumulates in the body with age, and uses Ex: 325 nm and Em: 420 nm as aging markers. It was decided to do.

  FIG. 3 is a graph showing an increase in fluorescence intensity with aging of an aging model organism. The horizontal axis indicates the wavelength, and the vertical axis indicates the fluorescence intensity. As FIG. 3 shows, each of several curve (1)-(9) shows the fluorescence intensity in a different measurement period of an aging model organism. Specifically, it is a curve obtained by measuring the fluorescence intensity every three days from the third day of life to every second day until the age of 17 days reaching the end of life.

  The fluorescent markers were Ex: 325 nm and Em: 420 nm described above. In order to standardize the protein concentration, the protein concentration was quantified by the Bradford method, and the fluorescence intensity was measured at the measurement time as an equivalent amount of 0.2 mg / ml. As a result, as shown in the figure, the fluorescence intensity showed a fluorescence that increased from 3 days of age to 5 days of age. It was also observed that the fluorescence intensity was very similar from 7 days to 13 days of age. Since this aging model organism reaches the egg-laying stage from the age of 5 days, it is suggested that the production of fluorescent substances occurs in the body accompanying the change of the growth factor gradually generated in the body at this time. In addition, at 17 days of age, the aging model organisms have a longer life and show higher fluorescence intensity than the curves up to 15 days of age. From this, it is expected that at 17 days of age, there is a high possibility of being subjected to oxidative stress.

  From the results shown in FIG. 3, it was predicted that a fluorescent substance having a higher fluorescence intensity at 17 days of age was produced in the body by comparing 3 days and 17 days of age, and thus this fluorescent substance was identified. Specifically, the structure of the fluorescent material was analyzed using an analyzer (LC-MALDI QSTAR 5800 manufactured by ABSciex) called LC-MALDI. By comparing the structural analysis results of 3 days and 17 days by liquid chromatography, a fluorescent substance having a fluorescence intensity that is clearly recognized to increase at 17 days of age is separated using a column. The structure of was analyzed.

  From the peaks identified by the protein database (public database Swiss Prot), Elongation factor and Vitellogenin-2, 5, 6 were confirmed at 17 days of age (see FIG. 4). Elongation factor is known to oxidize with aging, and vitellogenin is a precursor protein of yolk hormone and has been reported to accumulate in the intestinal tract. In both proteins, a substance that has been AGEd with aging emits fluorescence.

  FIG. 5 is a diagram showing a fluorescence spectrum obtained by saccharifying a protein that increases with aging. The wavelength of the excitation light (Ex) is 325 nm. Here, Riboflavin, Elongation factor, and Vitellogenin were targeted as proteins that increase with aging. These proteins were saccharified by mixing with a glucose solution and incubating at 35 ° C. for 10 days. The plurality of curves shown in FIG. 5 show the fluorescence spectra of glycated substances of these proteins.

  As shown in FIG. 5, the fluorescence spectrum (Em) of the saccharified substance of the elongation factor shows that the fluorescence intensity is high at 420 nm. That is, it was confirmed that the saccharified substance of the elongation factor has a high correlation with fluorescent markers of Ex: 325 nm and Em: 420 nm. From this result, it was clarified that the fluorescent marker of Ex: 325 nm and Em: 420 nm is a fluorescent substance mainly composed of an elongation factor.

  Vitellogenin had a possibility as an aging marker in structural analysis, but it was confirmed that Ex: 325 nm, Em was not emitted as a molecular structure because the fluorescence intensity was low under excitation conditions of Ex: 325 nm. : Excluded from 420 nm fluorescent marker.

  Next, using the aging evaluation method as described above, fluorescence spectra obtained when two different aging model organisms of the same age were given different foods were measured. FIG. 6 is a diagram showing fluorescence spectra obtained when two different 7-year-old aging model organisms were fed with different foods. The wavelength of the excitation light (Ex) is 325 nm. One aging model organism A was fed with normal food, and the other aging model organism B was fed with food containing a long-life substance. The long-life substance was vitamin C.

  As shown in FIG. 6, the fluorescence intensity of the aging model organism B was lower than that of the aging model organism A. From this, it can be seen that in the aging model organism B, the elongation factor that increases with aging could be reduced. From this, it can be judged that administration of the long-life substance was suitable as an effect of suppressing the increase of the elongation factor, and it was proved that the aging evaluation method was correct. Furthermore, the aging evaluation method was able to obtain a guideline for a long life effect.

  Moreover, since this aging model organism can be applied to a human aging model, it is possible to obtain a guideline for the effect of prolonging the human life. As a method for promoting the longevity of humans, in addition to the method of administering a long-life substance, an antioxidant substance, coenzymes such as polyphenols, vitamins, carotenoids, glutathione, ubiquinol, uric acid or It is self-evident that screening for prolonging life by administering lipoic acid or the like to suppress protein oxidation, prolonging life by exercising, or prolonging life by suppressing stress is possible.

<Modification 1>
The aging evaluation apparatus in Modification 1 is configured to detect fluorescence when the evaluation target is a solution. FIG. 7 is a diagram illustrating an example of the configuration of the aging evaluation apparatus in the first modification. As illustrated in FIG. 7, the aging evaluation apparatus 100A includes a control device 1 and a measurement unit 110. In the measurement unit 110, an excitation light source 101, a detector 103, and a cell holder 111 are arranged in a casing. The excitation light source 101 irradiates excitation light toward the cell holder 111. A transparent container containing a solution-like evaluation object is disposed in the cell holder 111, and excitation light emitted from the excitation light source 101 passes through the solution-like evaluation object and enters the detector 103.

<Modification 2>
In the modification 2, it is a structure provided with the fingertip measuring apparatus in the aging evaluation apparatus 100 in this embodiment. Since other configurations and functions are the same as those of aging evaluation apparatus 100, description thereof will not be repeated here.

  FIG. 8 is a diagram illustrating an example of the configuration of the fingertip measuring apparatus. As shown in FIG. 8, the fingertip measuring apparatus 200 includes a fingertip insertion unit 210 and a measurement member arrangement unit 220. The measurement member arrangement unit 220 has a measurement table 211 that contacts the fingertip at a portion communicating with the fingertip insertion unit 210. The measurement table 211 is provided with a hole with a diameter of 5 to 10 mm in order to extract excitation light from the measurement probe 105 described later, and a quartz cover glass (not shown) is installed in the hole. The fingertip insertion part 210 has an insertion hole 215 and a space for inserting a fingertip.

  Further, in the measurement member arrangement portion 220, the end of the measurement probe 105 that is the exit side of the incident optical fiber 105a and the incident side of the exit optical fiber 105b is directed to the cover glass of the measurement table 211. It is arranged with. Thereby, the excitation light can be irradiated to the fingertip inserted from the fingertip insertion unit 210 and placed on the measurement table 211, and the light emitted from the fingertip by the irradiated excitation light can be guided to the detector 103. Therefore, the fluorescence can be detected.

  In addition, when the user inserts a finger into the fingertip insertion portion 210, the presence of the measurement table 211 prevents the finger from pushing the tip of the measurement probe 105. Therefore, the end portion and the target on the incident side of the outgoing optical fiber 105b The distance relationship with the object (fingertip) can be kept constant. The measurement table 211 is set to be larger than the diameter of the end portion on the incident side of the outgoing optical fiber 105b. This makes it possible to measure the blood vessel position of the fingertip using an infrared camera.

  The fingertip is a place where AGEd substances tend to accumulate. Therefore, the measurement accuracy can be increased by using the fingertip as a place for measuring the fluorescence emitted by the AGE substance. In addition, since melanin does not exist in the fingertip, it is not necessary to pay attention to absorption of excitation light by melanin during transdermal fluorescence measurement. That is, it is possible to evaluate the progress of aging by eliminating the effects of sunburn and the influence of different races (such as being able to measure both colored and white races).

<Modification 3>
The aging evaluation apparatus in Modification 3 is configured to irradiate excitation light toward the fingertip insertion unit 210 and detect fluorescence without using the measurement probe 105. Differences from the fingertip measuring apparatus 200 according to Modification 2 are mainly described. FIG. 9 is a diagram illustrating an example of the configuration of the aging evaluation apparatus in the third modification. As shown in FIG. 9, the excitation light source 101 and the detector 103 included in the aging evaluation apparatus 100B are arranged in the measurement member arrangement unit 220, and a reflector 213 is arranged below the fingertip insertion unit 210 in the horizontal direction. Arranged at a predetermined angle.

  The reflecting mirror 213 reflects the excitation light emitted from the excitation light source 101 and emits it toward the space of the fingertip insertion unit 210. For this reason, when a fingertip is inserted into the fingertip insertion unit 210, the fingertip can be irradiated with excitation light. In addition, the fluorescence emitted by irradiating the fingertip with the excitation light is emitted toward the space of the measurement member arrangement unit 220, reflected by the reflecting mirror 213, and incident on the detector 103.

<Modification 4>
The aging evaluation apparatus in Modification 4 is configured to include a measurement probe 105A having a tip bent 90 degrees as shown in FIG. 10, and the other configuration is the same as that of the aging evaluation apparatus 100 in the present embodiment. The explanation will not be repeated. Since the measurement probe 105A has its support fixed to a fixed shaft, the excitation light is basically generated downward. For this reason, it is possible to simplify the measurement in the aging model organism culture vessel. In addition, the 90 ° bend significantly reduces the concern of direct light entering the eye of the measurer.

[Summary]
As described above, the aging evaluation method according to one embodiment of the present invention evaluates the progress of aging based on a fluorescent substance that increases in the body with the aging of an organism.

  According to the above method, since the aging evaluation target is a fluorescent substance, an expensive apparatus such as gene analysis and complicated processing are unnecessary. And since it is only necessary to specify the fluorescent substance which increases in the body with aging, the evaluation of aging is simple, and it is possible to evaluate on the spot. Therefore, aging can be easily evaluated on the spot without using gene analysis or the like. In addition, an apparatus for measuring aging of skin due to glycation has been developed, and an application that can show the effects of anti-aging cosmetics and anti-aging supplements can be considered.

  In addition, the aging evaluation method according to one aspect of the present invention provides a fluorescence spectrum emitted from the fluorescent material characteristic of the aging period compared to the young period by irradiating the living organism with excitation light having a wavelength in a predetermined range. The progress of aging is evaluated by measuring the fluorescence intensity.

  In the aging evaluation method according to one embodiment of the present invention, the wavelength of the excitation light is preferably in the range of 305 to 365 nm.

  In the aging evaluation method according to one embodiment of the present invention, the fluorescence spectrum in which the fluorescent substance has the maximum fluorescence intensity is preferably included in the range of 400 to 470 nm ± half-value width.

  In the present embodiment and Modifications 1 to 4, a control circuit that always keeps the output of the excitation light constant may be added to make the measurement conditions constant. This is because a commercially available fluorescence spectrophotometer has a large apparatus and is not versatile, and space and cost restrictions are imposed on the measurement.

  The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope shown in the claims, and embodiments obtained by appropriately combining technical means disclosed in different embodiments. Is also included in the technical scope of the present invention.

  In addition, the aging evaluation apparatus may be configured as follows.

<A>
An aging evaluation apparatus for evaluating aging by using the aging evaluation method according to any one of the above aspects.

<B>
The degree of progress of aging by irradiating an organism with excitation light with a wavelength in a predetermined range and performing a predetermined calculation using the fluorescence intensity of the fluorescence spectrum emitted by a fluorescent substance characteristic of the old age compared to the young Aging determination means for outputting a determination result indicating
The aging evaluation apparatus according to <A>, further comprising display control means for displaying a determination result output by the aging determination means on a display device.

<C>
A fingertip insertion portion for inserting a fingertip;
A light source unit that emits the excitation light to the fingertip inserted in the fingertip insertion unit;
The aging evaluation apparatus according to <A> or <B>, further comprising a detection unit that detects the fluorescence spectrum generated by irradiating the excitation light.

  Moreover, the aging evaluation apparatus and the aging evaluation method may be configured as follows.

<1>
An aging evaluation apparatus that evaluates the progress of aging based on a fluorescent substance that increases in the body with the aging of a living organism, and a fingertip insertion unit for inserting a fingertip;
A measurement table for placing a fingertip; a light source unit that emits excitation light; and a detection unit that detects a fluorescence spectrum. The measurement table includes a translucent region at a predetermined position. The excitation light irradiated by the light is guided to the translucent area, and the detection unit is emitted by irradiating the fingertip disposed on the measurement table with the excitation light through the translucent area. An aging evaluation apparatus, wherein the fluorescence spectrum is detected.

<2>
<1> characterized by measuring the fluorescence intensity of the fluorescence spectrum emitted by the fluorescent material characteristic of the old age compared to the young age by irradiating the organism with excitation light having a wavelength in a predetermined range. The aging evaluation apparatus according to 1.

<3>
The wavelength of the said excitation light is the range of 305-365 nm, The aging evaluation apparatus as described in <2> characterized by the above-mentioned.

<4>
The aging evaluation apparatus according to <3>, wherein a fluorescence spectrum in which the fluorescent substance has a maximum fluorescence intensity is included in a range of 400 to 470 nm ± half-value width.

<5>
The degree of progress of aging by irradiating an organism with excitation light with a wavelength in a predetermined range and performing a predetermined calculation using the fluorescence intensity of the fluorescence spectrum emitted by a fluorescent substance characteristic of the old age compared to the young Aging determination means for outputting a determination result indicating
The aging evaluation apparatus according to any one of <1> to <4>, further comprising display control means for displaying a determination result output by the aging determination means on a display device.

<6>
An aging evaluation method executed in an aging evaluation apparatus for evaluating aging of an organism,
The aging evaluation apparatus includes an aging evaluation value calculation unit and an aging determination unit,
Based on the fluorescent substance that increases in the body as the organism ages, the aging evaluation calculation unit calculates an aging evaluation value indicating the degree of aging, and
The aging determination unit evaluates the degree of aging by specifying the degree of aging corresponding to the calculated aging evaluation value based on the aging evaluation data in which the aging evaluation value and the degree of aging are associated with each other. Including steps,
The method for evaluating aging, wherein the fluorescent substance is Riboflavin or an evolution factor.

<7>
<6> characterized by measuring the fluorescence intensity of the fluorescence spectrum emitted by the fluorescent substance characteristic of the old age compared to the young age by irradiating the organism with excitation light having a wavelength in a predetermined range. The aging evaluation method described in 1.

<8>
The aging evaluation method according to <7>, wherein the wavelength of the excitation light is in a range of 305 to 365 nm.

<9>
The aging evaluation method according to <8>, wherein a fluorescence spectrum in which the fluorescent substance has a maximum fluorescence intensity is included in a range of 400 to 470 nm ± half-value width.

DESCRIPTION OF SYMBOLS 1 Control apparatus 100,100A, 100B Aging evaluation apparatus 101 Light source for excitation (light source part)
103 Detector (Detector)
105, 105A Measuring probe 105a Incident optical fiber 105b Outgoing optical fiber 110 Measuring unit 111 Cell holder 200 Fingertip measuring device 210 Fingertip inserting part 211 Measuring base 213 Reflecting mirror 215 Inserting hole 220 Measuring member arranging part 300 Control part 311 Aging evaluation Value calculation unit (aging evaluation value calculation means)
313 Aging determination unit (aging determination means)

Claims (5)

An aging evaluation apparatus that evaluates the progress of aging based on fluorescent substances that increase in the body with the aging of living organisms,
A fingertip insertion portion for inserting a fingertip;
A measuring table for placing the inserted fingertip;
A light source unit that emits excitation light;
A detection unit for detecting fluorescence emitted by irradiating the fingertip with the excitation light;
An incident optical fiber that propagates the excitation light emitted by the light source unit;
The fluorescence is incident, and includes an output optical fiber connected to the detection unit,
The incident optical fiber and the outgoing optical fiber are provided coaxially,
The measuring table has a hole at a predetermined position,
The aging evaluation apparatus, wherein the incident optical fiber and the outgoing optical fiber are arranged toward the hole.   The aging evaluation apparatus according to claim 1, wherein a cover glass is provided in the hole.   The aging evaluation apparatus according to claim 1 or 2, wherein the hole has a diameter of 5 to 10 mm. The excitation light has a predetermined range of wavelengths,
4. The fluorescence intensity of a fluorescence spectrum emitted from the fluorescent substance characteristic of the old age as compared with the young age is measured by irradiating the living organism with the excitation light. 5. The aging evaluation apparatus according to claim 1.   The aging evaluation apparatus according to claim 4, wherein the fluorescent material is an elongation factor.

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