JP2015186460A - Detection method of organism - Google Patents
Detection method of organism Download PDFInfo
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- JP2015186460A JP2015186460A JP2014065050A JP2014065050A JP2015186460A JP 2015186460 A JP2015186460 A JP 2015186460A JP 2014065050 A JP2014065050 A JP 2014065050A JP 2014065050 A JP2014065050 A JP 2014065050A JP 2015186460 A JP2015186460 A JP 2015186460A
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Landscapes
- Catching Or Destruction (AREA)
Abstract
Description
本発明は、泡を利用することにより、生物、特に有害生物の存在を検知する方法に関するものである。 The present invention relates to a method for detecting the presence of organisms, particularly pests, by using bubbles.
生物を検知する方法としては、ゴキブリを静電容量の変化に応じて検出する方法、また害虫を発光素子と受光素子の対から成る複数のセンサを用いて検出する方法が行われている。しかし、この方法は複雑で費用のかかる機器を使用する方法であり、簡便性に欠けているという問題があった。また小動物としてダニを検知する方法として水溶性色素を用いる方法が提案されているが、発色させるためにシートを押圧する操作が必要であり、検出する操作が煩雑であるという問題があった。 As a method for detecting living organisms, there are a method for detecting cockroaches according to a change in capacitance, and a method for detecting pests using a plurality of sensors comprising pairs of light emitting elements and light receiving elements. However, this method uses a complicated and expensive device, and there is a problem that it lacks simplicity. In addition, a method using a water-soluble dye has been proposed as a method for detecting mites as a small animal, but there is a problem that an operation of pressing a sheet is required for color development, and the detection operation is complicated.
従って、本発明の目的は検知する部分の構造が単純であり、生物、特に有害生物の存在の検知することができる方法を提供することが課題である。 Therefore, an object of the present invention is to provide a method capable of detecting the presence of a living organism, particularly a pest, with a simple structure of a portion to be detected.
本発明者は鋭意研究を重ねた結果、発泡させるための基材を一種類以上配合した組成物を物理的または化学的作用により泡を生じさせ、その泡を生物的作用により消失させることを利用することによって、生物、特に有害生物の存在の検知が可能であることを見出した。 As a result of extensive research, the present inventor uses a composition containing one or more base materials for foaming to generate bubbles by physical or chemical action and to eliminate the bubbles by biological action. By doing so, it was found that the presence of organisms, particularly pests, can be detected.
すなわち、本発明は、（１）発泡させるための基材が一種類以上配合された組成物を物理的または化学的作用により泡を生じさせ、その泡を生物的作用により消失させることを利用することを特徴とする生物の検知方法であり、（２）前記の組成物に、泡を安定にするための安定剤または固定剤を一種類以上配合した組成物であることを特徴とする（１）の生物の検知方法であり、（３）前記の泡を平板状に形成させることを特徴とする（１）または（２）の生物の検知方法である。 That is, the present invention utilizes (1) generating a foam by a physical or chemical action in a composition containing one or more base materials for foaming, and eliminating the foam by a biological action. (2) A composition comprising one or more stabilizers or fixing agents for stabilizing foam in the composition (1) (3) The organism detection method according to (1) or (2), wherein the bubbles are formed in a flat plate shape.
発泡させるための基材を一種類以上配合した組成物を物理的または化学的作用により泡を生じさせ、その泡を生物的作用により消失させることに利用することで、検知する部分の構造が単純であり、生物、特に有害生物の存否検知することが可能となる。 By using a composition containing one or more base materials for foaming to generate bubbles by physical or chemical action and to eliminate the bubbles by biological action, the structure of the detected part is simple. Therefore, it is possible to detect the presence or absence of organisms, particularly pests.
本発明に使用する、発泡させるための基材としては、発泡させたものが一定の期間、存続できるものであれば特に限定されるものではないが、例えば、水溶性タンパク質、水溶性多糖類、界面活性剤、水溶性樹脂、ウレタンフォームや発泡スチロール等の発泡プラスチック等が挙げられる。水溶性タンパク質としては、卵タンパク質、大豆タンパク質、小麦タンパク質、米タンパク質、トウモロコシタンパク質、乳清タンパク質、ラクトグロブリン、コラーゲン、ベタイン、ムタステイン、氷核タンパク質、ラクトフェリン、ゼラチン、カゼイン、アビジン、モネリン、ミラクリン、ムチン、レクチン、プロトロンビン、糖タンパク質等を例示することができ、水溶性多糖類としては、寒天、ゲランガム、グアガム、ペクチン、カラギーナン、キサンタンガム、アラビアガム、タラガム、ローカストビーンガム等を例示することができる。 The base material for foaming used in the present invention is not particularly limited as long as the foamed material can survive for a certain period of time. For example, water-soluble proteins, water-soluble polysaccharides, Examples include surfactants, water-soluble resins, foamed plastics such as urethane foam and polystyrene foam. Examples of water-soluble proteins include egg protein, soybean protein, wheat protein, rice protein, corn protein, whey protein, lactoglobulin, collagen, betaine, mutstein, ice core protein, lactoferrin, gelatin, casein, avidin, monelin, miraculin, Examples include mucin, lectin, prothrombin, glycoprotein and the like, and examples of water-soluble polysaccharides include agar, gellan gum, guar gum, pectin, carrageenan, xanthan gum, gum arabic, tara gum, locust bean gum and the like. .
界面活性剤としては、アニオン性、非イオン性、カチオン性、両性のいずれでも差支えないが、発泡させやすいものとしてはアニオン性界面活性剤またはカチオン性界面活性剤が好ましい。またこれらの界面活性剤は、一種類を単独で使用しても差し支えないが、異なるイオン性のものを含め二種類以上を混合しても差し支えない。アニオン性界面活性剤としてはステアリン酸ナトリウム、パルミチン酸ナトリウム、ミリスチン酸ナトリウム、ラウリル酸ナトリウム、アルキルベンゼンスルホン酸ナトリウム、ナフタレンスルホン酸ナトリウム、ポリオキシエチレンアルキルフェノールスルホン酸ナトリウム、ラウリル硫酸ナトリウム、ラウリル硫酸アンモニウム、ラウリルリン酸ナトリウム等、カチオン性界面活性剤としては塩化ベンザルコニウム、塩化アルキルトリメチルアンモニウム、塩化ドデシルジメチルベンジルアンモニウム、塩化ジアルキルジメチルアンモニウム、塩化セチルピリジニウム等、非イオン系界面活性剤としてはソルビタン脂肪酸エステル、ショ糖脂肪酸エステル、ポリオキシエチレンアルキルエーテル、モノステアリン酸グリセリン、ラウリル酸エタノールアミド、ステアリン酸ジエタノールアミド、セタノール、ステアリルアルコール等、両性界面活性剤としてはラウリルジメチルアミノ酢酸ベタイン、ドデシルアミノメチルジメチルスルホプロピルベタイン、ラウロイルグルタミン酸ナトリウム、ラウリルジメチルアミンＮ‐オキシド等を挙げることができる。 The surfactant may be any of anionic, nonionic, cationic, and amphoteric, but an anionic surfactant or a cationic surfactant is preferable as a surfactant that easily foams. These surfactants may be used alone or in combination of two or more, including different ionic ones. Anionic surfactants include sodium stearate, sodium palmitate, sodium myristate, sodium laurate, sodium alkylbenzene sulfonate, sodium naphthalene sulfonate, sodium polyoxyethylene alkylphenol sulfonate, sodium lauryl sulfate, ammonium lauryl sulfate, lauryl phosphorus Examples of cationic surfactants include benzalkonium chloride, alkyltrimethylammonium chloride, dodecyldimethylbenzylammonium chloride, dialkyldimethylammonium chloride, and cetylpyridinium chloride. Sugar fatty acid ester, polyoxyethylene alkyl ether, glyceryl monostearate, lauryl Examples of amphoteric surfactants include ethanolamide, stearic acid diethanolamide, cetanol, and stearyl alcohol. Examples of amphoteric surfactants include lauryldimethylaminoacetic acid betaine, dodecylaminomethyldimethylsulfopropylbetaine, sodium lauroylglutamate, and lauryldimethylamine N-oxide. .
水溶性樹脂は、水溶性の高いポリマーを示し、例えばポリビニルアルコール、ポリアクリル酸、ポリアクリル酸塩、ポリエーテル、ポリビニルピロリドン、ポリエチレングリコール等を挙げることができる。これらの水溶性樹脂は、一種類を単独で使用しても差し支えないが、二種類以上を混合しても差し支えない。 The water-soluble resin indicates a highly water-soluble polymer, and examples thereof include polyvinyl alcohol, polyacrylic acid, polyacrylate, polyether, polyvinyl pyrrolidone, and polyethylene glycol. These water-soluble resins may be used alone or in combination of two or more.
本発明に使用する、安定剤または固定剤は、発泡させたものを安定に長期間保持させるために使用する添加剤であり、このような作用を持つものであれば特に限定されるものではないが、ゲル化剤、エチレンオキシド付加物、脂肪酸多価アルコール等が挙げられる。ゲル化剤としては、寒天、ゲランガム、ゼラチン、グアガム、ペクチン、カラギーナン、キサンタンガム、アラビアガム、タラガム、ローカストビーンガム等を例示することができ、エチレンオキシド付加物としては、ポリオキシエチレンポリオキシプロピレンアルキルエーテル、ポリオキシアルキレンアルキルエーテル、ポリオキシアルキレングリコール等を挙げることができ、脂肪酸多価アルコールとしては、ポリオキシエチレンソルビタンモノラウレート、ポリオキシエチレンソルビタンステアレート、ポリオキシエチレンスルビタンモノオレエート等を挙げることができる。 The stabilizer or fixing agent used in the present invention is an additive used for stably maintaining a foamed product for a long period of time, and is not particularly limited as long as it has such an action. However, a gelling agent, an ethylene oxide adduct, a fatty acid polyhydric alcohol and the like can be mentioned. Examples of the gelling agent include agar, gellan gum, gelatin, guar gum, pectin, carrageenan, xanthan gum, gum arabic, tara gum, locust bean gum and the like, and examples of the ethylene oxide adduct include polyoxyethylene polyoxypropylene alkyl ether , Polyoxyalkylene alkyl ether, polyoxyalkylene glycol, and the like. Examples of fatty acid polyhydric alcohols include polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan stearate, polyoxyethylene sulfitan monooleate, and the like. Can be mentioned.
本発明の泡を生じさせるための物理的または化学的作用としては特に限定されるものではないが、物理的作用としては、攪拌機等の混合機を用いて物理的に基材を撹拌して気体を混入させる方法が挙げられる。混合機としては特に制限されるものではないが、回転羽根を有した攪拌機、ホモミキサー、気体の吹き込みを行うポンプ等が挙げられる。混入あるいは吹き込む気体については特に制限されるものではなく、窒素、アルゴン、二酸化炭素等の不活性ガスあるいは空気が挙げられ、これらの中で空気、窒素、二酸化炭素が好ましい。化学的作用としては、化学反応によって発生するガスを用いて発泡させる方法であり、このような化学反応としては、例えば炭酸塩化合物と酸性化合物の混合による二酸化炭素を発生させる反応、炭酸塩化合物を加熱することによって二酸化炭素を発生させる反応、亜鉛やマグネシウム等の金属と酸を接触させることによって水素を発生させる反応、ポリウレタンフォームの合成に利用されるポリオールとジイソシアネートとの反応、等が挙げられる。 The physical or chemical action for generating the foam of the present invention is not particularly limited, but as the physical action, the substrate is physically agitated using a mixer such as a stirrer to form a gas. The method of mixing is mentioned. Although it does not restrict | limit especially as a mixer, The stirrer which has a rotary blade, a homomixer, the pump which blows in gas, etc. are mentioned. The gas to be mixed in or blown in is not particularly limited, and examples thereof include inert gas such as nitrogen, argon and carbon dioxide, or air. Among these, air, nitrogen and carbon dioxide are preferable. The chemical action is a method of foaming using a gas generated by a chemical reaction. Examples of such a chemical reaction include a reaction that generates carbon dioxide by mixing a carbonate compound and an acidic compound, and a carbonate compound. Examples thereof include a reaction for generating carbon dioxide by heating, a reaction for generating hydrogen by bringing a metal such as zinc or magnesium into contact with an acid, a reaction of a polyol and diisocyanate used for the synthesis of polyurethane foam, and the like.
泡を消失させるための生物的作用としては特に限定されるものではないが、生物的作用としては、対象とする生物による直接的な発泡物体の破壊または消泡、生物が持っている酵素活性による発泡物体の破壊または消泡が挙げられる。また発泡物体に対象とする生物の餌となる成分を含有させ、生物が発泡物体を喫食することによって発泡物体を破壊または消泡させることができる。生物的作用による泡の消失の場合には生物を検知する方法として利用することができる。 The biological action for eliminating the foam is not particularly limited, but the biological action is based on the direct destruction or defoaming of the foamed object by the target organism or the enzyme activity possessed by the organism. Examples include destruction or defoaming of foamed objects. Moreover, the foamed object can be destroyed or defoamed by containing a component serving as a bait for the target organism and eating the foamed object by the organism. In the case of disappearance of bubbles due to biological action, it can be used as a method for detecting living things.
検知すべき対象とする生物とは、ゴキブリ、アリ、シロアリ、クモ、ダニ、チャタテムシ、シバンムシ、ムカデ、ヤスデ、キクイムシ等の匍匐性昆虫類等、ネズミ、ウサギ、イタチ、シカ、クマ、サル、タヌキ、ハクビシン、イノシシ等の哺乳類、カラス、ハト、スズメ、ムクドリ、ヒヨドリ、トビ、ワシ、タカ等の鳥類、カエル、サンショウウオ、イモリ、ヤモリ、トカゲ、ヘビ、カメ等の爬虫類や両生類が挙げられ、特に有害生物を検知する対象として選択することが好ましい。 The target organisms to be detected are cockroaches, ants, termites, spiders, ticks, scallops, dwarf insects, centipedes, millipedes, bark beetles, etc., mice, rabbits, weasels, deer, bears, monkeys, raccoon dogs , Mammals such as raccoon and wild boar, birds such as crow, pigeon, sparrow, starling, bird, tiger, eagle and hawk, reptiles and amphibians such as frog, salamander, newt, gecko, lizard, snake and turtle, especially It is preferable to select the target for detecting pests.
発泡させた物体は、上記の生物によって破壊、あるいは生物が放出する物質または生物自身の作用によって泡が消失し、検知することが可能となる。例えば、ゴキブリ、クモ等の小動物の場合では移動する際に発泡させた物体に接触し泡が消失する。ダニ等の微小生物の場合には例えば死骸の分解物や放出する糞の作用によって発泡させた物体の材料が分解し、泡が消失する。シカ、イノシシのような大型動物の場合には、発泡させた物体を適当な大きさの構造体とし、この構造体を大型動物が接触あるいは踏むことによって破壊され、その接触した痕跡あるいは足跡によって検知することができる。発泡させた構造体は密度を小さくすることが可能であり、動物が通過、接触することによって容易に痕跡を残すことができる。発泡させた構造体の強度は、検知対象の動物の重量によって調節することができ、小型動物の場合には強度を低く調整し、大型動物の場合には強度を高くする。さらに発泡させる物体を、検知対象とする生物の餌となる材料で作成することにより、対象とする生物が発泡させた物質を喫食することによって消失し、生物の存在を検出することができる。この場合にも、発泡させることによって喫食量が少ない場合にも体積減少が大きくなり検知が容易となる。 The foamed object is destroyed by the above-mentioned organism, or the foam disappears by the action of the substance released by the organism or the organism itself, and can be detected. For example, in the case of a small animal such as a cockroach or a spider, the foam disappears upon contact with the foamed object when moving. In the case of micro-organisms such as ticks, the material of the foamed object is decomposed by the action of decomposed products of dead bodies and feces released, and the bubbles disappear. In the case of large animals such as deer and wild boar, a foamed object is made into a structure of an appropriate size, and this structure is destroyed when the large animal touches or steps on it, and it is detected by the traces or footprints of the contact. can do. The foamed structure can be reduced in density and can easily leave a trace when an animal passes and contacts. The strength of the foamed structure can be adjusted according to the weight of the animal to be detected. The strength is adjusted to be low for small animals, and the strength is increased for large animals. Further, by creating an object to be foamed with a material serving as food for the organism to be detected, the object organism disappears by eating the foamed material, and the presence of the organism can be detected. Also in this case, when the amount of eating is small by foaming, the volume reduction is increased and the detection becomes easy.
発泡された物体の泡が破壊あるいは消失したことを検知する方法としては、発泡物体の形状変化を目視で確認する方法がある。これは発泡させた物体の密度が小さいことから、対象とする生物を検知する感度が高くなるものである。また発泡物体が不透明であることを利用し、検知されたことを表示する物体を発泡物体で覆うことによって見えない状態に置き、発泡物体の体積が減少することによって、検知されたことを示す物体が見える状態に変化し、検知するものである。発泡物体が覆う、検知されたことを示す物体は目視できるものであれば特に制限されるものではなく、例えば着色されたシート、文字、絵、記号、模様等を表示したシートが挙げられ、発泡物体が消失することによってシートに表示された色、文字、絵、記号、模様を目視で認識することができるようになる。また検知されたことを示す物体は、発光するものであってもよく、例えばＬＥＤ光源が挙げられ、発泡物体が消失した時に光が透過し、目視によって認識することが可能となる。光源が発光する光について、可視光であれば目視により認識することができるが、可視光に限らず紫外光や赤外光であってもよい。可視光でない場合には目視による検知ではなく、光を検出する装置が必要となる。もちろん、可視光の場合に適当な検出装置も用いても構わない。 As a method of detecting that the foam of the foamed object has been destroyed or disappeared, there is a method of visually confirming the shape change of the foamed object. Since the density of the foamed object is small, the sensitivity for detecting the target organism is increased. In addition, by utilizing the opaque foamed object, the object indicating that it has been detected is placed in an invisible state by covering it with a foamed object, and the volume of the foamed object is reduced to indicate that it has been detected. Changes to a visible state and is detected. The object that indicates that the foamed object is covered and is detected is not particularly limited as long as it is visible, and examples thereof include a colored sheet, a sheet displaying characters, pictures, symbols, patterns, etc. When the object disappears, the color, characters, pictures, symbols, and patterns displayed on the sheet can be visually recognized. Moreover, the object which shows having detected may be what emits light, for example, an LED light source is mentioned, Light is permeate | transmitted when a foaming object lose | disappears, and it becomes possible to recognize visually. The light emitted from the light source can be visually recognized as long as it is visible light, but is not limited to visible light, and may be ultraviolet light or infrared light. If it is not visible light, a device for detecting light rather than visual detection is required. Of course, a detection device suitable for visible light may be used.
発泡物体を調製するにあたり、対象とする生物によって泡の大きさ、発泡物体の破壊されやすさは適宜調整される。発泡物体は、生物的作用が及ばない限り安定して存在することが望ましいが、少なくとも生物の検知を目的とする期間安定して発泡された状態を維持するものであれば適用することが可能である。生物の検知を目的とする期間としては、１０日以上、好ましくは１か月以上、さらに好ましくは３か月以上である。 In preparing the foamed object, the size of the foam and the ease of destruction of the foamed object are appropriately adjusted depending on the target organism. It is desirable that the foamed object be stably present as long as the biological action is not exerted, but it is possible to apply the foamed object as long as the foamed object maintains a stable foamed state for at least the purpose of detecting the organism. is there. The period for detecting the organism is 10 days or longer, preferably 1 month or longer, more preferably 3 months or longer.
生物の検知、あるいは化合物の消費を検知する発泡物体は密度が低いことが特徴であり、物理的、化学的または生物的作用により消失させることによって体積変化が大きくなる。発泡物体の密度は、好ましくは１．０ｇ／ｃｍ３未満、より好ましくは０．５ｇ／ｃｍ３以下である。 Foamed objects that detect living organisms or consumption of compounds are characterized by low density, and their volume change increases when they disappear due to physical, chemical or biological action. The density of the foamed object is preferably less than 1.0 g / cm 3, more preferably 0.5 g / cm 3 or less.
本発明を利用することで、生物、特に有害生物の存在を検知するための方法について実施例を挙げて説明するが、本発明はこれらに限定されるものではない。 A method for detecting the presence of a living organism, particularly a pest, by using the present invention will be described with reference to examples, but the present invention is not limited thereto.
（実施例１）
ゼラチン１０ｇとイオン交換水９０ｇを混合して６０℃に加温し、溶解させた。溶解させた溶液を撹拌機（東京理科機械株式会社製 ２ＲＴ）にて９００ｒｐｍ、１時間撹拌して泡立たせた後、直径９ｃｍ、高さ１．７ｃｍの透明なプラスチックシャーレに１０ｇ分注したものを室温にて放熱し、泡立たせたゲルを調製した。
(Example 1)
10 g of gelatin and 90 g of ion-exchanged water were mixed, heated to 60 ° C. and dissolved. The dissolved solution was bubbled by stirring at 900 rpm for 1 hour with a stirrer (Tokyo Science Machine Co., Ltd. 2RT), and then dispensed 10 g into a transparent plastic petri dish having a diameter of 9 cm and a height of 1.7 cm. A foamed gel was prepared by releasing heat at room temperature.
（実施例２）
ゼラチン５ｇとイオン交換水９５ｇを混合して６０℃に加温し、溶解させた。溶解させた溶液を撹拌機（東京理科機械株式会社製 ２ＲＴ）にて９００ｒｐｍ、１時間撹拌して泡立たせた後、直径９ｃｍ、高さ１．７ｃｍの透明なプラスチックシャーレに１０ｇ分注したものを室温にて放熱し、泡立たせたゲルを調製した。
(Example 2)
5 g of gelatin and 95 g of ion-exchanged water were mixed, heated to 60 ° C. and dissolved. The dissolved solution was bubbled by stirring at 900 rpm for 1 hour with a stirrer (Tokyo Science Machine Co., Ltd. 2RT), and then dispensed 10 g into a transparent plastic petri dish having a diameter of 9 cm and a height of 1.7 cm. A foamed gel was prepared by releasing heat at room temperature.
（実施例３）
ゼラチン１０ｇとイオン交換水９０ｇを混合して６０℃に加温し、溶解させた。溶解させた溶液をホモミキサー（特殊機化工業株式会社製 Ｔ．Ｋ．ＨＯＭＯ ＭＩＸＥＲ ＭＡＲＫＩＩ）にて５０００ｒｐｍ、１時間撹拌して泡立たせた後、直径９ｃｍ、高さ１．７ｃｍの透明なプラスチックシャーレに１０ｇ分注したものを室温にて放熱し、泡立たせたゲルを調製した。
(Example 3)
10 g of gelatin and 90 g of ion-exchanged water were mixed, heated to 60 ° C. and dissolved. The dissolved solution was bubbled by stirring at 5000 rpm for 1 hour with a homomixer (TK KOH MIXER MARKII, manufactured by Tokushu Kika Kogyo Co., Ltd.), and then a transparent plastic petri dish having a diameter of 9 cm and a height of 1.7 cm. A 10 g aliquot was radiated at room temperature to prepare a foamed gel.
（試験例１）
実施例１〜３で調製したプラスチックシャーレを幅４０ｃｍ、奥行き２７ｃｍ、高さ１２ｃｍの容器内に設置したものを２つ用意し、一方はチャバネゴキブリを１０頭放虫し、もう一方には何もせず、室温にて３日間静置した。３日後に実施例１〜３の泡の消失を目視にて観察を行った。観察結果は下記の基準で評価した。結果を表１に示す。
○ 泡の消失が目視で確認できる
× 泡の消失が目視で確認できない
(Test Example 1)
Prepare two petri dishes prepared in Examples 1 to 3 in a container with a width of 40 cm, a depth of 27 cm and a height of 12 cm, one releasing 10 cockroaches and the other without doing anything. And allowed to stand at room temperature for 3 days. Three days later, the disappearance of the foams of Examples 1 to 3 was visually observed. The observation results were evaluated according to the following criteria. The results are shown in Table 1.
○ The disappearance of bubbles can be confirmed visually.
× The disappearance of bubbles cannot be confirmed visually
（試験例２）
実施例１〜３で調製したプラスチックシャーレを２つずつ用意し、一方にはコナヒョウヒダニ５００匹を放虫し、もう一方には何もせず、室温にて３日間静置した。３日後に実施例１〜３の泡の消失を目視にて観察を行った。結果を表２に示す。
○ 泡の消失が目視で確認できる
× 泡の消失が目視で確認できない
(Test Example 2)
Two plastic petri dishes prepared in Examples 1 to 3 were prepared, one of which was radiated with 500 white leopard mites, and the other was left unattended at room temperature for 3 days. Three days later, the disappearance of the foams of Examples 1 to 3 was visually observed. The results are shown in Table 2.
○ The disappearance of bubbles can be confirmed visually.
× The disappearance of bubbles cannot be confirmed visually
（試験例３）
実施例１〜３で調製したプラスチックシャーレを２つずつ用意し、一方にはヒラタコクヌストモドキ１０匹を放虫し、もう一方には何もせず、室温にて３日間静置した。３日後に実施例１〜３の泡の消失を目視にて観察を行った。結果を表３に示す。
○ 泡の消失が目視で確認できる
× 泡の消失が目視で確認できない
(Test Example 3)
Two plastic petri dishes prepared in Examples 1 to 3 were prepared, 10 of which were released on one side, and nothing was done on the other side, and were allowed to stand at room temperature for 3 days. Three days later, the disappearance of the foams of Examples 1 to 3 was visually observed. The results are shown in Table 3.
○ The disappearance of bubbles can be confirmed visually.
× The disappearance of bubbles cannot be confirmed visually
（試験例４）
実施例１〜３で調製したプラスチックシャーレを２つずつ用意し、一方にはヒラタチャタテムシ２０匹を放虫し、もう一方には何もせず、室温にて３日間静置した。３日後に実施例１〜３の泡の消失を目視にて観察を行った。結果を表４に示す。
○ 泡の消失が目視で確認できる
× 泡の消失が目視で確認できない
(Test Example 4)
Two plastic petri dishes prepared in Examples 1 to 3 were prepared, 20 flat-spotted beetles were released on one side, nothing was done on the other side, and left at room temperature for 3 days. Three days later, the disappearance of the foams of Examples 1 to 3 was visually observed. The results are shown in Table 4.
○ The disappearance of bubbles can be confirmed visually.
× The disappearance of bubbles cannot be confirmed visually
本発明の生物の検知方法を用いることで、単純な検知部分の構造及び単純な製造工程の検出方法を用いて高感度に生物を検知することができる。
By using the organism detection method of the present invention, it is possible to detect organisms with high sensitivity using a simple detection part structure and a simple manufacturing process detection method.
Claims (3)
3. The organism detection method according to claim 1, wherein the bubbles are formed in a flat plate shape.
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