JP2015181452A - Method for preventing epidemics of fishes - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for enhancing a prevention ability of epidemics of fishes inexpensively and conveniently.SOLUTION: A method for preventing epidemics of fishes of the invention comprises administering to fishes a feed, which contains high-protein rapeseed meal and fish meal with a protein content of 40 to 50%, and in which the high-protein rapeseed meal to the total amount of the high-protein rapeseed meal and the fish meal is 10-50% by weight, to enhance the epidemic ability of the fishes. According to the invention, particularly bactericidal activity of blood serum is enhanced from a young fish or fry stage.

Description

  The present invention relates to a fish prevention method, and more particularly to a method for preventing fish from being easily and inexpensively protected.

  In fish farming sites, fish farms may suffer from fish diseases such as viral infections, bacterial diseases, and fungal diseases, resulting in massive death. The decrease in catch due to the occurrence of fish diseases is wasteful of the cost and time spent on aquaculture such as fry, feed and labor costs. Conventionally, antibiotics, antibacterial agents, vaccines, chemicals and the like have been administered as a preventive measure. However, administration of antibiotics and the like is a concern for problems such as induction of antibiotic-resistant bacteria, burden on environmental water, cost, and increased labor during administration.

  It is desired to increase the immune activity of fish farms and prevent the occurrence of diseases. Currently, administration of immunostimulatory substances such as β-glucan, peptidoglycan, lipopolysaccharide, chitin / chitosan, and lactoferrin to fish is practiced. In addition, yeast containing astaxanthin (Patent Document 1), inactivated dead cells derived from bacteria belonging to the genus Lactococcus, etc., cell disruptions derived from Gram-positive bacteria (Patent Document 2), polys containing specific basic amino acids Peptide (Patent Document 3), Vitamin, Digestive Enzyme, Combination of Live Bacteria and Immunostimulant (Patent Document 4), Peptide Obtained by Mixed Culture of Bacillus thuringes and Bacillus pumilus (Patent Document 5), Yeast Administration of yeast cells treated with a cell wall lytic enzyme (Patent Document 6), a combination of a specific cytokine and an enteric protective polymer (Patent Document 7), etc. has also been proposed.

  In the aquaculture field, it is also important for fish to efficiently ingest the protein source as a nutritional component of the feed to promote growth. Conventionally, fish meal is a protein source commonly used in fish feed. However, fish meal has a problem that its market price is easily influenced by the amount of fish catch, so that search for fish meal substitute raw materials has been conducted, and soybean meal and rapeseed meal are known as alternative protein sources. However, since rapeseed meal has low protein content and digestibility, it has been difficult to use it for fish whose protein requirement is higher than that of cattle, pigs and other livestock.

JP 2002-080351 A JP 2001-342140 A JP2000-325026 JP 2001-190231 A JP 2000-103740 JP-A-8-280332 JP2012-505193

  It is an object of the present invention to provide a method for enhancing the prevention of epidemics of fish at low cost and in a simple manner. Another object of the present invention is to provide a method for improving rapeseed meal that has not been suitable as a fish meal substitute material for fish feed because of its low nutrient intake.

  As a result of intensive research on the above problems, the present inventors have found that the above two problems can be solved simultaneously by using a feed containing a specific amount of rapeseed meal having a specific protein content, and have completed the present invention. That is, the present invention is a feed containing a high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and the fish meal is 10 to 30% by weight. A method for the prevention of fish is provided, comprising administering to the fish the feed that is%, to enhance the prevention of the disease.

  The epidemic prevention ability is, for example, serum bactericidal activity.

  It is preferable that the feed further includes blending soybean meal.

  It is preferable that the ratio of the soybean meal to the total of the soybean meal and the fish meal is 10 to 30% by weight.

  The inventors have also found that the muscle lipids of fish bred by the above method are significantly increased. Therefore, the present invention is also a feed containing a high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and the fish meal is 10 to 10%. Provided is a method for regulating fish muscle lipid, comprising administering to a fish the feed that is 30% by weight.

  The present invention also includes a high protein rapeseed meal having a protein content of 40 to 50% and fish meal, and the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and the fish meal is 10 to 30% by weight. Provide feed.

  It is preferable that the feed for fish farming further contains soybean meal.

  The ratio of the soybean meal to the total of the soybean meal and the fish meal is preferably 10 to 30% by weight.

  The present invention also provides a high protein rapeseed meal and fish meal having a protein content of 40 to 50% such that the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal is 10 to 30% by weight. Provided is a method for producing a feed for fish farming, comprising blending.

  It is preferable that the method for producing fish feed further includes blending soybean meal.

  It is preferable that the ratio of the soybean meal to the total of the soybean meal and the fish meal is 10 to 30% by weight.

  According to the method of the present invention, serum bactericidal activity and leukocyte phagocytic ability are enhanced as compared with fish meal feed. In addition, the amount of protein in the body surface mucus decreases, which means an increase in mucopolysaccharides and an increase in the prevention of epidemics. In particular, the bactericidal activity of serum is enhanced more than when a portion of fish meal is replaced with conventional rapeseed meal. Moreover, since this enhancement is exhibited from the time of juvenile or fry, the superiority of the method of the present invention is high.

  When a fish is raised using a feed in which a part of the fish meal is replaced with a conventional rapeseed meal, the weight increase rate (growth) of the fish is clearly inferior to that of a fish meal-based feed with a uniform protein content. That is, the intake rate of nutrients derived from rapeseed meal is poor. On the other hand, according to the method of the present invention using a feed in which part of the fish meal is replaced with high-protein rapeseed meal, the rate of weight increase of the fish is improved.

  Although the protein content of the high protein rapeseed meal used in the method of the present invention is lower than that of fish meal, it is improved to the same extent as soybean meal. And the manufacturing cost of the feed used by this invention is cheap compared with a fish meal main body feed.

  Fish reared by the method of the present invention has a high muscle lipid accumulation action. Thereby, the value of livestock and fishery products can be raised.

  Therefore, the method of the present invention has sufficient industrial utility value as an alternative to feed mainly composed of fish meal.

Fish meal-based feed (control), feed in which part of fish meal is replaced with conventional rapeseed meal (Comparative Examples 1 and 2), and feed in which part of fish meal is replaced with high-protein rapeseed meal (Examples 1 and 2) The result of measuring muscle lipid (CL) after 4 weeks when using flounder for 4 weeks using is shown. In Comparative Examples 1 and 2, there is a decrease from the control. On the other hand, in Examples 1 and 2, muscle lipid is remarkably increased. As in FIG. 1, flounder was obtained using a fish meal-based feed (control), a feed replaced with conventional rapeseed meal (Comparative Examples 1 and 2), and a feed replaced with high protein rapeseed meal (Examples 1 and 2). The result of having measured the phagocytosis rate of the leukocyte of the fish after 2 weeks (2W) and 4 weeks (4W) when fed for 4 weeks is shown. In Example 2 and Comparative Example 2, the phagocytosis rate of leukocytes is enhanced compared to the fish meal-based feed (control). As in FIG. 1, flounder was obtained using a fish meal-based feed (control), a feed replaced with conventional rapeseed meal (Comparative Examples 1 and 2), and a feed replaced with high protein rapeseed meal (Examples 1 and 2). The result of measuring the protein amount in the body surface mucus of the fish after 2 weeks (2W) and 4 weeks (4W) when fed for 4 weeks is shown. In Comparative Examples 1 and 2 and Examples 1 and 2, the decrease in the amount of protein in the body surface mucus (increase in polysaccharides) is stronger than the control. In particular, in Example 2, the amount of protein in the body surface mucus decreased from the early stage of the second week. Therefore, according to the method of the present invention, it is possible to cultivate fish with high epidemiological ability by administering a feed in which a part of the fish meal is replaced with high protein rapeseed meal from the time of juvenile or fry. As in FIG. 1, flounder was obtained using a fish meal-based feed (control), a feed replaced with conventional rapeseed meal (Comparative Examples 1 and 2), and a feed replaced with high protein rapeseed meal (Examples 1 and 2). The results of measuring serum bactericidal activity (E. coli survival rate) after 2 weeks and 4 weeks when fed for 4 weeks are shown. The bactericidal activity of the sera of Comparative Examples 1 and 2 is not much different from the control group. On the other hand, both Examples 1 and 2 have enhanced bactericidal activity at 4 weeks. In particular, in Example 2, since the bactericidal activity is high from the early stage of the 2nd week, by administering a feed in which a part of the fish meal is replaced with a high protein rapeseed meal from the time of juvenile or fry, it is possible to prevent epidemics. High-quality fish can be raised.

  Hereinafter, one embodiment of the method of the present invention will be described in detail. The target of the fish epidemic prevention method of the present invention is not particularly limited as long as it is a fish. The method of the present invention is particularly applicable to farmed fish. Cultured fish include flounder, red sea bream, black sea bream, sea bream, yellowtail, yellowtail, amberjack, tuna, tiger pufferfish, striped mackerel, sea mackerel, Japanese flounder, riverfish, scorpion, rockfish, black sea bream, sea bass, sea bream, sea mackerel, cod, coho salmon, Thailand Includes Atlantic salmon, rainbow trout, carp, crucian carp, eel, catfish, etc.

  The feed contains fish meal as a protein source. As the fish meal, conventional ones can be used without particular limitation. The proportion of fish meal in the feed is usually 30 to 70% by weight, preferably 40 to 60% by weight.

  The feed used in the method of the present invention contains a high protein rapeseed meal having a protein content of 40 to 50% by weight, preferably 40 to 48% by weight, particularly preferably 40 to 46% by weight, based on water content. If the protein content is less than 40%, the effects of the present invention may be reduced, leading to deterioration in growth or a decrease in the survival rate. Conversely, if the protein content exceeds 50%, the burden on the environment due to increased nitrogen excretion (water pollution) Etc.).

  The crude lipid of the high protein rapeseed meal is usually 5% or less, preferably 1 to 4%, more preferably 1.5 to 3%.

  The crude fiber content of the high protein rapeseed meal is usually 8% or less, preferably 1 to 7%, more preferably 1 to 6%.

  Of the crude fibers, NDF (neutral detergent fibers) is usually 20% or less, preferably 18% or less. ADF (acid detergent fiber) is usually 15% or less, preferably 13.4% or less. Lignin is usually 4% or less, preferably 3% or less.

  The glucosinolate content of the high protein rapeseed meal is usually 30 μmol / g or less, preferably 20 μmol / g or less, more preferably 10 μmol / g or less.

  High protein rapeseed meal can be produced by rapeseed molting, washing, enzyme treatment, alcohol treatment, sieving treatment, and the like. Among them, the sieving classification product when the sieving treatment is performed with a sieve of 32 mesh (aperture 500 μm) or less, preferably 48 mesh (aperture 300 μm) or less, the protein content, the crude lipid, and the coarse fiber are within the above preferred ranges. It is preferable in that it can be easily obtained.

  The ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and the fish meal is 10 to 50% by weight, preferably 10 to 30% by weight. If the said ratio is 10 weight% or less, the epidemic prevention ability of fish will not be strengthened effectively. On the other hand, if it exceeds 50% by weight, a large amount of other protein sources are required to secure the amount of protein in the feed, and there is a problem that crude fibers are added more than necessary.

  The feed is preferably supplemented with other protein sources to supplement the protein content reduced by the use of high protein rapeseed meal. Examples include soybean meal, molted soybean meal, soybean protein concentrate, isolated soybean protein, rapeseed meal, cottonseed meal, lupine seed meal, corn distilled meal, corn gluten meal, corn gluten feed, alfalfa powder, potato Plant protein sources such as protein, chickpea, pea, kidney bean, lentil and black bean; animal protein sources such as meat and bone meal, blood meal, feather meal, pork meal, chicken meal and skim milk powder. Soybean meal is preferred because it supplements lysine, which is often deficient in high protein rapeseed meal.

The ratio of the soybean meal to the total of the soybean meal and the fish meal is usually:
It is 10 to 30% by weight, preferably 15 to 26% by weight. If the ratio is less than 10%, the effect is reduced. Conversely, if it exceeds 50%, the growth may be poor or the food intake may be reduced.

  In addition to fish meal, high-protein rapeseed meal, and other protein sources, those feeds that are widely used as raw materials for fish feed can be used without particular limitation. Such examples include grains such as rice, brown rice, rye, wheat, barley, corn, milo, soybean; vegetable oils, animal fats, powdered beef tallow, liver oils and other fats; lysine, methionine, taurine, Amino acids such as arginine; vitamins such as vitamin B1, vitamin B2, vitamin B6, vitamin B12, calcium pantothenate, nicotinamide, folic acid, vitamin C, biotin, choline and the like; vitamin-like substances; zinc, calcium, selenium, Minerals such as iron and phosphorus; inorganic salts such as magnesium sulfate, iron sulfate, copper sulfate, zinc sulfate, potassium iodide, cobalt sulfate, calcium carbonate, tricalcium phosphate, sodium chloride, calcium phosphate and choline chloride; Can be mentioned.

  For the feed, a general-purpose feed additive used for preventing deterioration of feed quality, promoting effective use of nutrient components, and the like may be used as long as the effects of the present invention are not impaired. Examples include antioxidants, fungicides, binders, emulsifiers, pH adjusters, antibacterial agents, flavoring agents, flavoring agents, enzymes, viable bacteria agents, organic acids, food attractants, yeast Etc.

  The feed used in the method of the present invention can be obtained by blending the above raw materials. The shape of the feed can be an extruded pellet, a dry pellet, a moist pellet, a paste, and the like.

  The method of the present invention enhances the serum bactericidal activity of fish and the phagocytosis rate of leukocytes compared to fish meal-based feed. Body surface mucus is secreted from epithelial cells and goblet cells and covers the outermost layer of the epidermis. Body surface mucus is mainly composed of mucopolysaccharides, and plays a particularly important role as a bacterial disease defense mechanism. The method of the present invention reduces the amount of protein in the body surface mucus of fish, which means an increase in mucopolysaccharide in the mucus. Mucopolysaccharides protect against bacteria and viruses that invade from the outside, so a decrease in protein content leads to an increase in the prevention of epidemics. The method of the present invention can be expected to improve the resistance of fish to streptococcal disease, nodular disease, nocardiosis, vibrio disease, iridovirus infection, edwardieria disease, parasitic diseases and the like.

  The present inventors have also found that the muscle lipids of fish bred by the above method are remarkably increased, and that this result can be used to regulate fish muscle fat, particularly up-regulation. Therefore, the present invention is also a feed containing a high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and the fish meal is 15 to A method for regulating muscle lipids in fish is provided, comprising administering to the fish the feed that is 30% by weight.

Hereinafter, the present invention will be described in more detail using Examples and Comparative Examples. However, the present invention is not limited to the following examples.
[Examples 1 and 2]
1. Preparation of feed As a control plot, a control feed mainly composed of fish meal (product name Fishmeal, manufactured by Nihon Suisan Co., Ltd.) was prepared with the composition shown in Table 1. Examples 1 and 2 were prepared by replacing 20% and 30% of the fish meal of the control diet with high protein rapeseed meal and soybean meal, respectively. The ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal was 17.2% in Example 1 and 25.0% in Example 2. The protein content reduced by replacing fish meal with high-protein rapeseed meal was supplemented with the addition of soybean meal (product name: soybean oil meal, manufactured by J-Oil Mills Co., Ltd.). The ratio of soybean meal to the total soybean meal and fish meal was 15.8% in Example 1 and 25% in Example 2.

  For comparison, feeds in which 20% and 30% of the fish meal of the control feed were replaced with conventional rapeseed meal (product name: rapeseed oil meal, manufactured by J-Oil Mills Co., Ltd.) were prepared as Comparative Examples 1 and 2, respectively. The ratio of rapeseed meal to the total of rapeseed meal and fish meal was 18.6% in Comparative Example 1 and 26.3% in Comparative Example 2. The protein content reduced by replacing fish meal with rapeseed meal was supplemented with the addition of soybean meal.

  The amount of cod liver oil added in each case was adjusted so that the oil content in all cases was the same. The amount of flour added in each example was adjusted so that the energy in all cases was the same. Table 2 shows the components of fish meal and substitute protein. Table 3 shows the contents of crude protein, crude lipid and amino acid in each feed.

High protein rapeseed meal: rapeseed meal (manufactured by J-Oil Mills Co., Ltd.) 48 mesh (mesh opening 300 μm) sieve classification vitamin MIX: β carotene, vitamin D3, vitamin K3, vitamin E, vitamin B1, vitamin A mixture of B6, vitamin B12, ascorbic acid, biotin, inositol, nicotinic acid, pantothenic acid, folic acid, choline chloride, and p-aminobenzoic acid (manufactured by Kokin Chemical Co., Ltd.)
Mineral MIX: NaCl, MgSO ₄ · 2H ₂ O, NaHPO ₄ · 2H ₂ O, KH ₂ PO ₄ , Fe Citrate, Calactate, Al (OH) ₃ , ZnSO ₄ · 2H ₂ O, CuSO ₄ , MnSO ₄ · 5H A mixture of ₂ O, Ca (IO ₃ ) ₂ , and CoSO ₄ · 7H ₂ O (manufactured by Kokin Chemical Co., Ltd.)
Stay-C: Ascorbyl monophosphate (DSM)

2. Rearing conditions Flounder flounder was reared in the rearing environment shown in Table 4.

3. Measuring growth indicators
In the control, comparative example, and example groups, fish body weights were measured 2 and 4 weeks after administration of the feed, respectively. Furthermore, the amount of food intake and the rate of weight gain were determined by the following formulas.

Table 5 shows the measurement results of fish weight, food intake and weight gain.

  When Table 5 is seen, the weight increase rate (growing condition) of Comparative Examples 1 and 2 is clearly inferior to the control. That is, when the fish meal is replaced with the conventional rapeseed meal, the nutrient intake rate deteriorates. On the other hand, in Examples 1 and 2, the weight increase rate is improved as compared with the comparative example by using the high protein rapeseed meal. For this reason, the method of the present invention in which part of the fish meal is replaced with a high protein rapeseed meal can be an alternative to a feed mainly composed of fish meal.

4). Measurement of components of growing fish One animal was sampled every 2 weeks (2W) before administration of the feed (Initial), and 4 weeks (4W). Dissect fish and determine specific liver weight, liver crude lipid, muscle crude protein (CP), crude lipid (CL), ash and moisture according to Kjeldahl method, Soxhlet method, direct ashing method and atmospheric pressure heating drying method It was.

The results of measuring the components of the fish are shown in Table 6.

  When Table 6 is seen, in the comparative examples 1 and 2 using the conventional rapeseed meal, both muscle CP and muscle CL are reducing from the control | contrast. On the other hand, in Examples 1 and 2 using the high protein rapeseed meal, the muscle CP is lowered and the muscle CL is raised. The muscle CL after 4 weeks is shown in FIG. The method of the present invention can regulate the muscle lipid by the function of increasing the muscle lipid of the fish, and enhances the value of the fish.

  The liver is generally a lipid accumulating organ, and when a vegetable protein is fed, lipid tends to accumulate in the liver. When the amount of lipid is excessive, the values of GOT and GPT are increased, resulting in an unhealthy state. In the examples, the specific liver weight decreased or remained constant for 2 weeks (2W) to 4 weeks (4W) after administration, whereas in Comparative Example 2, it increased rapidly. The liver crude lipid is not significantly different at 4 weeks (4 W) after administration, but the comparative example is not preferable because it is excessive in lipid when administered over a longer period together with enlargement of the liver.

5. Measurement of the epidemic prevention ability of growing fish (1) Leukocyte phagocytosis After 2 weeks (2W) and 4 weeks (4W) of feed administration, fish were captured and leukocyte phagocytosis was determined by leukocyte isolation method (Percoll discontinuous density gradient centrifugation). Based on the above, it was calculated by the following formula.

  Specifically, the activity was determined by the following procedure. The kidney is put into a mesh (100 mesh) (comment: it is said that it can be left as it is) and filtered with 3 mL of RPMI 1640 (manufactured by SIGMA) to prepare a leukocyte suspension. Percoll solutions (manufactured by GE Healthcare Japan) were prepared at 1.040 g / mL and 1.070 g / mL. In addition to the leukocyte suspension, the lower layer leukocytes were collected by centrifugation.

Yeast (Saccharomyces cerevisiae, manufactured by SIGMA) was opsonized and diluted with RPMI1640. 0.5 mL of this mixed solution was mixed with 0.5 mL of flounder serum and incubated at 25 ° C. for 1 hour. After centrifugation (3000 rpm, 4 ° C., 15 min), 0.8 mL of the supernatant was removed. After adding 0.8 mL of RPMI 1640 and pipetting, centrifugation was repeated twice. 200 μL of this reaction solution was taken and 0.8 mL of RPMI 1640 was added to prepare 1 × 10 ⁶ cells / mL. This yeast solution and leukocyte suspension were mixed at 200 μL each and incubated at 25 ° C. for 2 hours. 200 μL of this mixed solution was smeared on a slide glass and stained with Giemsa. After drying, the phagocytosis rate was measured with a microscope for staining.

  Furthermore, the leukocyte phagocytosis rate of each example was determined as a relative value when the control was 100. Higher numbers mean better epidemiological performance than controls.

(2) Protein mass in body surface mucus When secretion of body surface mucus increases, the main component polysaccharide increases, resulting in a decrease in protein mass. The increase in polysaccharides contributes to fish prevention. Therefore, the amount of protein in the body surface mucus is an index indicating the anti-quarantine ability of fish. Therefore, fish were captured 2 weeks (2W) and 4 weeks (4W) after administration of the feed, and the protein amount was measured based on the Lowry method. Specifically, the activity was determined by the following procedure.

  Five fish were sampled from each test group, and mucus secreted in a certain area of the body surface was scraped with a sterilized absorbent cotton a certain number of times. The absorbent cotton was collected in a microtube containing PBS, and the mucus was suspended. The supernatant was collected by centrifugation at 3000 rpm × 10 minutes at room temperature, and the protein in the supernatant was measured by the Lowry method.

  The numerical value of each example was calculated | required as a relative value when making a control | contrast 100. A lower numerical value means a higher proportion of polysaccharides and therefore higher epidemiological ability.

(3) Serum bactericidal activity Serum bactericidal activity is an indicator of the anti-quarantine ability of fish. Therefore, fish were captured 2 weeks (2W) and 4 weeks (4W) after administration of the feed, and the bactericidal activity of the serum (E. coli survival rate) was determined by the method of Yamamoto et al. (Yamamoto, A. et.al., Fish Pathology, vol.30, 1995, p123-124). Specifically, the activity was determined by the following procedure.

Escherichia coli is cultured in TSA medium, and 2 to 3 mg is fished in a test tube with a lid, and further diluted 1000 times with Tris buffer to a concentration of 1 mg / mL. E. coli suspension. 50 μL of serum in one of the two microtubes and E. After adding 50 μL of E. coli suspension, 150 μL of Tris buffer was added to each, and pipetting was performed, and 100 μL was transferred to another microtube. There are 100 μL of Tris buffer and E. coli. 200 μL of E. coli suspension was added and incubated at 24 ° C. for 1 hour. 400 μL of the reaction solution was serially diluted every 10 times with physiological saline up to 10 ^{2 to 5} times to form a 4-step dilution series. The dilution series was inoculated 50 μL at a time into TSA medium and spread with a congeal rod. The blank is E.I. E. coli suspension and Tris buffer were added to each 200 μL microtube and mixed, and 100 μL of the mixture was incubated and diluted in the same manner as the sample, and smeared on TSA medium. Culturing at 25 ° C., counting the number of colonies and calculating the number of viable bacteria. The survival rate of E. coli was determined. Note that blank E.I. The survival rate of E. coli was 100%.

  The numerical value of each example was calculated | required as a relative value when making a control | contrast 100. A lower number means higher activity than the control.

  A one-way analysis of variance was performed using Super ANOVA, and when a significant difference was observed, multiple comparisons of average values were performed by the Turkey-Kramer method. A p value <0.05 was determined to be significant.

The leukocyte phagocytosis rate, the amount of protein in the body surface mucus, and serum bactericidal activity are shown in Table 7 and FIGS.

  In Comparative Examples 1 and 2, the leukocyte phagocytosis rate is stronger than the control. In addition, the amount of protein in the body surface mucus (increase in polysaccharide) is lower than that of the control. However, the bactericidal activity of serum is not much different or worse than the control group. In Examples 1 and 2, the phagocytosis rate of leukocytes is enhanced and the bactericidal activity is enhanced in addition to the decrease in the amount of protein in the body surface mucus. From this point, the method of the present invention is superior to the method using a normal rapeseed meal in that it comprehensively enhances the prevention of epidemiology in terms of leukocyte phagocytosis rate, protein mass in body surface mucus and bactericidal activity of serum. I can say that.

  In Example 2 in which 30% of the fish meal was replaced with high protein rapeseed meal and soybean meal, the amount of protein in the body surface mucus decreased and the bactericidal activity increased from the early stage of the second week. I understand. From this, it can be said that according to the method of the present invention, it is possible to cultivate fish with high epidemiological ability by administering a feed obtained by substituting a portion of fish meal with high protein rapeseed meal from the time of juvenile or fry.

Claims (11)

  A feed comprising a high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal is 10 to 50% by weight. A method for the prevention of fish, comprising administering to fish to enhance the ability to prevent the prevention of fish.   The fish epidemic prevention method according to claim 1, wherein the epidemic prevention ability is a bactericidal activity of serum.   The method according to claim 1, wherein the feed further comprises blending soybean meal.   The method according to claim 3, wherein a ratio of the soybean meal to the total of the soybean meal and the fish meal is 15 to 30% by weight.   A feed comprising a high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal is 10 to 50% by weight. A method for regulating muscle lipids in fish, comprising administering to fish.   A feed for fish farming comprising high protein rapeseed meal and fish meal having a protein content of 40 to 50%, wherein the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal is 10 to 50% by weight.   The fish feed according to claim 6, further comprising soybean meal.   The feed for fish farming of Claim 7 whose ratio of the said soybean meal with respect to the sum total of the said soybean meal and the said fish meal is 15-30 weight%.   Including a high protein rapeseed meal and fish meal having a protein content of 40 to 50% such that the ratio of the high protein rapeseed meal to the total of the high protein rapeseed meal and fish meal is 10 to 50% by weight, A method for producing fish feed.   Furthermore, the manufacturing method of the feed for fish farming of Claim 9 including mix | blending soybean meal.   The manufacturing method of the feed for fish farming of Claim 10 whose ratio of the said soybean meal with respect to the sum total of the said soybean meal and the said fish meal is 10-30 weight%.