JP2015139487A - Platelet preparation preservation device and platelet preparation preservation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a platelet preparation preservation device and a platelet preparation preservation method capable of extending a preservation period of the platelet preparation.SOLUTION: A platelet preparation preservation device 10 includes: a platelet preparation accommodation part 12 for accommodating a platelet preparation preservation container 21 for preserving a platelet preparation; and a three-dimensional clinostat 11 for biaxially rotating the platelet preparation accommodation part 12. By the biaxial rotation of the platelet preparation accommodation part 12, the platelet preparation preservation device 10 can uniformly agitate platelets in the platelet preparation at a constant load in three dimensions. Thereby, since the platelet preparation can be preserved while suppressing the aggregation of platelets, the quality of the platelet preparation can be maintained and the preservation period of the platelet preparation can be extended.

Description

  The present invention relates to a platelet preparation storage device and a platelet preparation storage method.

  There are platelet products for blood transfusion that are used for patients who need blood transfusion. If the platelet preparation for blood transfusion is not stored in a state of being shaken horizontally, oxygen permeation becomes insufficient, and the metabolism of platelets tends to become anaerobic, resulting in a decrease in pH or a decrease in platelet function. Therefore, the platelet preparation for blood transfusion is preserve | saved, for example using a horizontal shaker, a horizontal and rotating shaker, etc. (for example, refer patent document 1). In addition, the expiration date of platelet preparations for blood transfusion is generally set to 4 days after blood collection. Thus, the storage conditions of the platelet preparation for transfusion include other erythrocyte preparations (21 days after blood collection, storage temperature: 2 to 6 ° C.) and plasma preparations (one year after blood collection, storage temperature: −20 ° C. or lower). It is stricter than blood products, and the expiration date of transfusion platelet products is also shorter than other blood products.

  In addition, as a device for proliferating and culturing bone marrow stromal cells (BMSCs) or mesenchymal stem cells (MSCs) in an undifferentiated state, the applicant of the present invention can use the target cells in a simulated microgravity environment generated by multiaxial rotation. Three-dimensional clinostats that rotate in three dimensions and perform cell culture such as culturing bone marrow stromal cells and mesenchymal stem cells and producing transplanted cells for treatment of central nervous system diseases have been proposed (for example, patent documents) 2).

Japanese Utility Model Publication No. 3-75829 JP 2010-246434 A

  From the viewpoint of effectively using the platelet preparation for blood transfusion, it is desirable to further extend the storage period of the platelet preparation for blood transfusion. However, even if the platelet preparation for blood transfusion is stored under horizontal shaking conditions, problems such as a decrease in platelet function occur as the storage period of the platelet preparation for blood transfusion becomes longer, which is clinically undesirable. Therefore, there is a need for a technique for extending the storage period of a platelet preparation for blood transfusion without reducing the platelet function.

  The present invention has been made in view of the above, and an object of the present invention is to provide a platelet preparation storage device and a platelet preparation storage method capable of suppressing a decrease in function of a platelet preparation.

  The present invention for solving the above-described problems includes a platelet preparation storage portion for storing a platelet preparation storage container for storing a platelet preparation, and rotating the platelet preparation storage portion by n-axis (n is an integer of 2 or more, Preferably, it is an integer of 2 or more, 10 or less, more preferably an integer of 2 or more and 5 or less, and further preferably 2.).

  In the present invention, the rotating device includes a holding unit that holds the platelet product storage unit, a first rotating body that is connected to the holding unit and holds the holding unit, and the first rotating body that rotates the first rotation. A first driving unit that rotates about a shaft; a second rotating body that rotatably holds the first rotating body around a first rotating shaft; and an axial center of the first rotating shaft. A leg portion rotatably held around a second rotating shaft having an axial direction in a direction different from the direction, and a second driving portion that rotates the second rotating body around the second rotating shaft. It is preferable to have.

  In this invention, it is preferable that the said platelet formulation storage part has a pair of partition part which partitions off the several said platelet formulation storage container inside the said platelet formulation storage part.

  In the present invention, it is preferable that the holding portion includes a pair of holding plates facing each other and a plurality of connecting portions that connect the holding plate and the first rotating body, and is formed in a box shape.

  Further, the present invention is characterized in that a platelet preparation storage container for storing a sample containing platelets is rotated in the n-axis direction (n is an integer of 2 or more) to store the platelets while suppressing aggregation of the platelets. And a method for preserving the platelet preparation.

  In the present invention, the rotating device includes a holding unit that holds the platelet product storage unit, a first rotating body that is connected to the holding unit and holds the holding unit, and the first rotating body that rotates the first rotation. A first driving unit that rotates about a shaft; a second rotating body that rotatably holds the first rotating body around a first rotating shaft; and an axial center of the first rotating shaft. A leg portion rotatably held around a second rotating shaft having an axial direction in a direction different from the direction, and a second driving portion that rotates the second rotating body around the second rotating shaft. It is preferable to have.

  By using the platelet preparation storage device of the present invention, it is possible to suppress a decrease in function of the platelet preparation. By using the method for preserving a platelet preparation of the present invention, it is possible to suppress a decrease in function of the platelet preparation.

FIG. 1 is a diagram showing an example of a platelet preparation storage device according to an embodiment of the present invention. FIG. 2 is a view showing a platelet preparation storage container and a platelet preparation storage part. FIG. 3 is an explanatory diagram showing a coordinate system defined for the platelet preparation storage device. FIG. 4 is a graph showing platelet-rich plasma (PRP) immediately after blood collection and PRP platelet count after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 5 is a graph showing PRP immediately after blood collection and the average platelet volume (MPV) of PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 6 is a graph showing PRP immediately after blood collection and the platelet distribution width (PDW) of PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 7 is a graph showing the PRP clot retraction rate immediately after blood collection and the PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 8 is a graph showing the low osmotic shock recovery rate of PRP immediately after blood collection and PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 9 is a diagram showing the platelet aggregation reaction of PRP immediately after blood collection and PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2. FIG. 10 is a graph showing the PRP immediately after blood collection and the PRP platelet count after storage by the methods of Example 2 and Comparative Example 3. FIG. 11 is a diagram showing PRP immediately after blood collection and MPV of PRP after storage by the method of Example 2 and Comparative Example 3. FIG. 12 is a diagram showing PRP immediately after blood collection and PDW of PRP after storage by the method of Example 2 and Comparative Example 3. FIG. 13 is a graph showing the platelet aggregation reaction of PRP immediately after blood collection and PRP after storage by the methods of Example 2 and Comparative Example 3.

  Hereinafter, a form for carrying out a platelet product storage device and a platelet product storage method according to the present invention (hereinafter referred to as an embodiment) will be described in detail with reference to the drawings. In addition, this invention is not limited by the following embodiment. In addition, constituent elements in the following embodiments include those that can be easily assumed by those skilled in the art, those that are substantially the same, and those in a so-called equivalent range. Furthermore, the constituent elements disclosed in the following embodiments can be appropriately combined.

[Embodiment]
<Platelet preparation storage device>
A platelet preparation storage device according to an embodiment of the present invention will be described. FIG. 1 is a diagram showing an example of a platelet preparation storage device according to an embodiment of the present invention. FIG. 2 is a view showing a platelet preparation storage container and a platelet preparation storage part.

  The platelet preparation storage device 10 includes a three-dimensional clinostat (rotating device) 11 and a platelet preparation storage unit 12. In the present embodiment, platelets from animals such as humans and rabbits are used as the platelet preparation.

  The three-dimensional clinostat 11 includes a holding portion 13, an inner frame (first rotating body) 14, an outer frame (second rotating body) 15, a leg portion 16, a first motor (first driving portion) 17, and a second motor ( 2nd drive part) 18 is provided.

  The holding unit 13 holds the platelet preparation storage unit 12. The holding part 13 includes a pair of opposing holding plates 13a and a plurality of connecting parts 13b, and is formed in a box shape. Each of the pair of holding plates 13a is a flat plate and is disposed so as to face a position sandwiching the inner frame 14 therebetween. That is, the two holding plates 13a are arranged in parallel so that the surfaces (front surfaces) having the largest areas face each other. One end of the connecting portion 13b is connected to the inner frame 14, and the other end is connected to one of the holding plates 13a. The connecting portion 13b is connected to any one of the four corners of the holding plate 13a. In the present embodiment, the connecting portion 13b is fixed to the holding plate 13a with screws. Each of the four corners of the holding plate 13a is connected to the connecting portion 13b. The holding plate 13a is fixed to the inner frame 14 by the connecting portion 13b. The holding part 13 fixes the two holding plates 13a to the inner frame 14 by a plurality of connecting parts 13b. In addition, in the holding unit 13, the platelet preparation storage unit 12 is disposed in a space surrounded by the two holding plates 13 a. The holding part 13 fixes the platelet product containing part 12 between the pair of holding plates 13a by sandwiching the platelet product containing part 12 between the pair of holding plates 13a. In the present embodiment, the holding unit 13 supports and fixes the platelet product storage container 12 by screwing, but the mechanism by which the holding unit 13 supports and fixes the platelet product storage container 12 is not particularly limited. You may fix by methods other than screwing. The distance between the pair of opposing holding plates 13a can be adjusted to any length by adjusting the length of the connecting portion 13b. For this reason, the holding | maintenance part 13 can be suitably adjusted to arbitrary length according to the length etc. of the platelet formulation storage container 12 pinched | interposed with the holding | maintenance part 13. FIG.

  The inner frame 14 is a quadrangular frame. The inner frame 14 holds the platelet preparation housing part 12 via the holding part 13. The inner frame 14 rotates around the first rotation axis α. In the present embodiment, the first rotation axis α is an axis that passes through the rotation axis of the first motor 17. The direction of rotation of the inner frame 14 may be either clockwise or counterclockwise.

  The outer frame 15 is a quadrangular frame composed of four side portions 15a, and the holding portion 13 and the inner frame 14 are arranged inside the frame. The outer frame 15 holds a pair of opposing sides 15a among the four sides 15a so that the inner frame 14 can rotate around the first rotation axis α. The outer frame 15 is held by the legs 16 so that the other pair of opposing sides 15a among the four sides 15a can rotate around the second rotation axis β. The second rotation axis β is an axis perpendicular to the first rotation axis α. The rotation direction of the outer frame 15 may be either clockwise or counterclockwise.

  The leg portion 16 holds the outer frame 15 so that it can rotate around the second rotation axis β. The leg portion 16 is fixed to the gantry 19. The first motor 17 rotates the inner frame 14 around the first rotation axis α.

  The first motor 17 is provided outside one side 15a of the pair of sides 15a. The first motor 17 rotates the inner frame 14 around the first rotation axis α via a power transmission mechanism such as a gear or a belt.

  The second motor 18 is provided on one leg 16 of the pair of legs 16. The second motor 18 rotates the outer frame 15 around the second rotation axis β via a power transmission mechanism such as a gear or a belt.

  In the present embodiment, the three-dimensional clinostat 11 is configured such that the first rotation axis α and the second rotation axis β are substantially orthogonal to each other. The inner frame 14, the outer frame 15, and the leg portion 16 constitute a gimbal mechanism that can be tilted in any direction by combining the first rotation axis α and the second rotation axis β that are orthogonal to each other. Note that the first rotation axis α and the second rotation axis β are not limited to be orthogonal to each other, and the first rotation axis α and the second rotation axis β have a predetermined angle. It may be provided.

  Next, the platelet preparation container 12 will be described with reference to FIG. The platelet preparation storage unit 12 is a container that can store a plurality of platelet preparation storage containers 21. Here, the platelet preparation storage container 21 is a container for storing the platelet preparation, and includes a sample enclosure 23 for enclosing the platelet preparation. The platelet preparation storage container 21 includes a lid 26 detachable from the platelet preparation storage container 21 in the sample enclosure 23. The platelet preparation storage container 21 is closed with the lid 26 after the platelet preparation is enclosed in the sample enclosure 23. The platelet preparation storage container 21 stores the platelet preparation therein by enclosing the platelet preparation in the sample enclosure 23. The method of encapsulating the platelet preparation in the sample enclosure 23 is not limited to the method of providing the removable lid 26 on the sample enclosure 23, and the sample enclosure 23 is provided with an openable / closable lid. 23 may contain a platelet preparation.

  The platelet product storage unit 12 includes a plurality of partition parts 24 inside the platelet product storage unit 12. The partition part 24 is formed at intervals corresponding to the width of the platelet preparation storage container 21. The platelet preparation storage part 12 can hold the platelet preparation storage container 21 inside by inserting the platelet preparation storage container 21 between the partition part 24 and the partition part 24. Further, since the platelet product storage unit 12 stores the plurality of platelet product storage containers 21 by partitioning the partition 24, the position of the platelet product storage container 21 in the platelet product storage unit 12 can be fixed, and The platelet preparation storage container 21 can be easily attached and detached. The platelet product container 12 is in the vicinity of the intersection of the first rotation axis α and the second rotation axis β. Since the platelet preparation storage part 12 is held by the holding part 13, it rotates integrally with the inner frame 14.

The three-dimensional clinostat 11 rotates the inner frame 14 around the first rotation axis α by the first motor 17, and rotates the outer frame 15 around the second rotation axis β by the first motor 17, so that the platelet product container 12 Is rotated two axes. The three-dimensional clinostat 11 generates a pseudo weightless state by combining the first rotation axis α and the second rotation axis β and rotating the platelet preparation storage container 21 in three dimensions. In addition, the pseudo-gravity state is a microgravity (weightlessness) state where the temporal average of gravity at the center material installation position is about 1 × 10 ⁻³ G by controlling the rotational speeds of two axes orthogonal to each other. Say. The three-dimensional clinostat 11 can maintain the quality of the platelet preparation and can increase the storage period of the platelet preparation by placing the platelet preparation in a pseudo-weightless state. Here, since the direction of gravity acting on the platelets in the platelet preparation is dispersed by rotating the platelet preparation container 12 biaxially with the three-dimensional clinostat 11, the present inventors distribute the platelets in the platelet preparation. It can be uniformly stirred with a constant load in three dimensions. For this reason, it is considered that the three-dimensional clinostat 11 can reduce the stress applied to the platelets as compared with the case where the platelet preparation is shaken horizontally using a commonly used horizontal shaker. As a result, the three-dimensional clinostat 11 can suppress the decrease in platelet aggregation function and store the platelet preparation, so that the quality of the platelet preparation can be maintained and the storage period of the platelet preparation can be extended. Think of what you can do.

  The quality of the platelet preparation includes, for example, the number of platelets in the platelet preparation, mean platelet volume (MPV), platelet distribution width (PDW), clot retraction rate, hypoosmotic shock recovery rate, and platelets. Examples include agglutination reaction. The increase or decrease in the number of platelets is used as an indicator of platelet damage. MPV and PDW serve as indices indicating changes in platelet morphology. The platelet count, MPV and PDW are measured using, for example, a conventionally known automatic blood cell counter. The clot retraction rate is an indicator of platelet function. The clot retraction rate is determined by adding a predetermined amount of thrombin solution to the platelet preparation and measuring it after a predetermined time has elapsed. The low osmotic pressure shock recovery rate is obtained by adding distilled water to a platelet preparation and measuring the change in platelet morphology and the recovery state after a predetermined time as a change in turbidity. The platelet aggregation reaction is determined by adding a predetermined concentration (for example, 10 μM) of adenosine diphosphate (ADP) to the platelet preparation and measuring the aggregation reaction by a turbidimetric method.

  In the present embodiment, the three-dimensional clinostat 11 rotates the platelet preparation storage container 12 in two axes. However, the present invention is not limited to this, and the platelet preparation storage container 12 is further connected to a plurality of rotation axes (for example, N-axis rotation: n is an integer of 2 or more, preferably an integer of 2 or more and 10 or less, more preferably an integer of 2 or more and 5 or less, and still more preferably 2. You may make it do. Even in this case, as in the case of the biaxial rotation, the direction of gravity acting on the platelets in the platelet preparation is dispersed by the rotation of the platelet preparation storage container 12, and each platelet is uniformly stirred with a constant load in three dimensions. Can do.

(Control of the rotation of the three-dimensional clinostat 11)
Next, an example in which the platelet preparation storage device 10 generates a pseudo weightless state by controlling the rotation of the three-dimensional clinostat 11 will be described. FIG. 3 is an explanatory diagram showing a coordinate system defined by the platelet preparation storage device 10. In FIG. 3, the first rotation axis α and the second rotation axis β corresponds to the X ₀ axis and Y ₀ axis. In the present embodiment, a stationary reference coordinate system {P ₀ , X ₀ , Y ₀ , Z ₀ } fixed to the gantry 19 is defined. A coordinate system {P ₁ , X ₁ , Y ₁ , Z ₁ } fixed to the outer frame 15 is defined, and a coordinate system {P ₂ , X ₂ , Y ₂ fixed to the inner frame 14 is defined. , Z ₂ } is defined. Note that the origin of the coordinate system coincide _{is P 0 = P 1 = P 2} . X ₀ axis and _{Y 0} axis oriented in the horizontal direction, _{Z 0} axis is vertically downward.

The platelet preparation storage device 10 includes a rotation angle detector 31, a rotation angle detector 32, and a control device 35 having a motor control unit (motor controller) 33 and a trajectory generation unit 34. Rotation angle detector 31 detects the rotation angle theta of about Y ₀ axis with respect to the gantry 19 of the outer frame 15, and outputs the motor control unit 33 as the detected rotation angle theta ^S. Rotation angle detector 32 detects the rotation angle phi around the X ₁ axis relative to the outer frame 15 of the inner frame 14, and outputs the motor control unit 33 as the detected rotation angle phi ^S. The trajectory generating unit 34 outputs the rotation angle command θ ^* and the rotation angle command φ ^* to the motor control unit 33. The motor control unit 33 outputs a torque command τ _θ ^* to the motor controller 33 so that the detected rotation angle θ ^S matches the rotation angle command θ ^*, and matches the detected rotation angle φ ^S with the rotation angle command φ ^*. Torque command τ _φ ^* is output to the motor controller 34. The motor controller 33 rotates the outer frame 15 around the _{Y 0} axis as the torque command tau _theta ^*. The motor controller 34 rotates the inner frame 14 about _{the X 1} axis in accordance with the torque command tau _phi ^*.

で表される。ここで、上付き文字「Ｔ」は、「転置」を意味している。 Here, P ₀ , X ₀ , Y ₀ , Z ₀ are represented by the formula (Equation 1):

It is represented by Here, the superscript “T” means “transpose”.

で表される。 The coordinate system {X ₁ , Y ₁ , Z ₁ } when the outer frame 15 rotates about the Y ₀ axis by the rotation angle θ is expressed by the following equation (Equation 2):

It is represented by

で表される。 The coordinate system {X ₂ , Y ₂ , Z ₂ } when the inner frame 14 rotates about the X ₁ axis by the rotation angle φ is an expression (Equation 3):

It is represented by

で表される。また、Ｒ_Ｘは、Ｘ_１軸まわりの回転を表す座標変換行列であり、式（数５）：

で表される。 Here, R _Y is a coordinate transformation matrix representing rotation around the Y ₀ axis, and the equation (Equation 4):

It is represented by R _X is a coordinate transformation matrix that represents rotation about the X ₁ axis, and the equation (Equation 5):

It is represented by

で表される。 From the above equation, the coordinate system fixed with respect to the inner frame 14 is the equation (Equation 6):

It is represented by

及び式（数８）：

が成立する。 Assuming that the angular velocity vector of the outer frame 15 is ω ₁ and the angular velocity vector of the inner frame 14 is ω ₂ , Expression (Equation 7):

And the formula (Equation 8):

Is established.

で表される。 Therefore, the angular acceleration vector α ₂ of the inner frame 14 is expressed by the formula (Equation 9):

It is represented by

で表される。 Therefore, the magnitude of the angular acceleration α ₂ of the inner frame 14 to which the sample is fixed is expressed by the following equation (Equation 10):

It is represented by

で表される。
ここで、Ｍ、Ｎは、任意の正の整数ｍに対して式（数１２）：

を満たす。また、ｂ_１及びｂ_２ｋ＋１は、式（数１３）：

の解である。 The trajectory generation unit 34 outputs a rotation angle command θ ^* and a rotation angle command φ ^* indicating θ (t) and φ (t), respectively, to the motor control unit 33. θ (t) and φ (t) are expressed by Equation (11) with respect to time t, predetermined period T, predetermined coefficients b ₁ , b _{2k + 1} , and predetermined positive integers k, M, and N:

It is represented by
Here, M and N are expressions (Expression 12) for an arbitrary positive integer m:

Meet. In addition, b ₁ and b _{2k + 1} are expressed by the following equation (Equation 13):

Is the solution.

  When the platelet product storage device 10 is controlled based on the formula (Equation 11), the time average of the gravity vector acting on the sample becomes zero, and the influence of the gravity acting on the sample is equal to three axes orthogonal to each other. And a pseudo-weightless state is generated.

In equation (11), because the rotational angular acceleration ^{d 2 φ} / dt ² about _{the X 1} axis is zero, as is clear from equation (10), the angular magnitude of the acceleration alpha ₂ of the inner frame 14 Becomes smaller.

  Further, since the trajectory defined by the formula (Equation 11) is expressed by an analytical formula, it is easy to predict what the trajectory will be when parameters such as M and N are changed. .

Furthermore, since the trajectory defined by the equation (Equation 11) has periodicity, it is easy to set the operating conditions of the platelet product storage device 10 when an experiment is performed in a pseudo-weightless state. For example, the experimenter determines the period T so that the experiment time is a positive integer multiple of the period T, and rotates the rotation around the Y ₀ axis and the rotation around the X ₁ axis during the period T. Therefore, the parameters M and N may be determined.

Further, in the trajectory defined by the equation (11) is the same even in the continuous infinite order differential function at any time t, Y ₀ rotation angle around the axis and around the X ₁ axis, the rotational angular velocity, angular acceleration Is prevented from changing rapidly. Therefore, vibration is prevented from occurring when the platelet preparation storage device 10 is operated.

  Hereinafter, the derivation of Expression (Expression 11) to Expression (Expression 13) will be described.

が成立すればよい。 In order for the gravitational acceleration components g _X , g _Y , and g _Z in the three axes of the coordinate system {P ₂ , X ₂ , Y ₂ , Z ₂ } to cancel out in time and become zero, (n−1) In each section of the cycle T expressed by T ≦ t ≦ nT (n = 1, 2,...), The formula (Equation 14):

Should just hold.

が成立すればよい。 Further, in order for the influence of gravity acting on the sample to be evenly distributed on the three axes orthogonal to each other, the formula (Equation 15):

Should just hold.

で表される。したがって、式（数１４）で与えられた重力が時間的に相殺される条件は、式（数１７）〜式（数１９）：

で表される。また、式（数１５）で与えられた重力の均等分散の条件は、式（数２０）：

で表される。 Here, gravitational acceleration components g _X , g _Y , and g _Z are expressed by the following equation (Equation 16):

It is represented by Therefore, the conditions under which the gravity given by the equation (Equation 14) is canceled in terms of time are the equations (Equation 17) to (Equation 19):

It is represented by Further, the condition for the uniform dispersion of gravity given by the equation (Equation 15) is the equation (Equation 20):

It is represented by

で表される。 In the equation (Equation 20), since the sum of the integrands is 1, the conditions for the uniform dispersion of gravity are the equations (Equation 21) to (Equation 23):

It is represented by

で表される。 In order to obtain, as a periodic function, a trajectory that satisfies Equation (Equation 17) to Equation (Equation 19) and Equation (Equation 21) to Equation (Equation 23), the rotational speeds around the Y ₀ axis and X ₁ axis during the period T Let M rotation and N rotation respectively. At this time, the boundary condition for each period is expressed by equation (Equation 24):

It is represented by

Here, the Y ₀ when the rotational angular velocity about the axis around or X ₁ axis is constant, because the angular acceleration about each axis is zero, the angular acceleration alpha ₂ represented by the equation (10) size Can be reduced.

に示すようにＹ_０軸まわりの回転角速度を一定とした場合に、式（数１７）〜式（数１９）及び式（数２１）〜式（数２３）の条件を満たすかどうか検討する。 Therefore, the formula (Equation 25):

If a constant rotational angular velocity around Y ₀ axis as shown in, the consider whether conditions are satisfied or equation (17) to (Expression 19) and (Expression 21) to (Expression 23).

に示されるように式（数２１）を満たさない。 In this case, Equation (Equation 17) is satisfied, but when the left side of Equation (Equation 21) is calculated, Equation (Equation 26):

Does not satisfy the equation (Equation 21).

に示されるようにＸ_１軸まわりの回転角速度を一定とした場合に、式（数１７）〜式（数１９）及び式（数２１）〜式（数２３）の条件を満たすかどうかを検討する。 Next, the equation (Equation 27):

In the case where the rotational angular velocity around the X ₁ axis constant as shown in, consider if satisfying one of the equation (17) to (Expression 19) and (Expression 21) to (Expression 23) To do.

が得られる。 When φ (t) expressed by the equation (Equation 27) is substituted into the equation (Equation 22), the equation (Equation 28):

Is obtained.

のように計算される。 Here, the sum of the first and third terms on the right side of the equation (Equation 28) is the equation (Equation 29):

It is calculated as follows.

で表される。ここで、係数ａ_０及びａ_ｍは、式（数３１）：

で表される。 Sin ² θ included in the fourth term on the right side of the equation (Equation 28) is an even function. Since the basic angular frequency of sin θ is 2πM / T because the outer frame 15 rotates M around the Y ₀ axis during the period T, the basic angular frequency of sin ² θ squared with sin θ is 4πM / T. T. At this time, since sin ² θ can be generally expressed by an even function Fourier series, the equation (Equation 30):

It is represented by Here, the coefficients a ₀ and a _m are expressed by the equation (Equation 31):

It is represented by

のように表される。 By substituting equation (Equation 30) for the integrand of the fourth term on the right side of Equation (Equation 28), the fourth term on the right side of Equation (Equation 28) becomes Equation (Equation 32):

It is expressed as

に示すように、式（数２８）の右辺第４項はゼロとなる。 Here, if the rotational speeds M and N are selected so that the formula (formula 12) is established for an arbitrary positive integer m, the formula (formula 33):

As shown, the fourth term on the right side of the equation (Equation 28) is zero.

が得られる。ここで、式（数３４）を整理すれば式（数２１）と同じ形になる。 By substituting Equation (29) and Equation (33) into Equation (28), Equation (34):

Is obtained. Here, if formula (Formula 34) is arranged, it becomes the same form as Formula (Formula 21).

  Therefore, if φ (t) is defined by the equation (Equation 27) under the boundary condition for each period shown in the equation (Equation 24) and θ (t) satisfying the equation (Equation 21) is obtained, the obtained θ (T) satisfies the equation (Equation 22) at the same time.

  From the same way of thinking, it can be seen that θ (t) satisfying the equation (Equation 21) also satisfies the equation (Equation 23).

  Therefore, θ (t) that satisfies the equation (Equation 21) is determined.

When theta of (t) is expressed by equation (25), because the angular acceleration about the rotation around Y ₀ axis does not occur, theta (t) is preferably close to the function shown in equation (25) . If the sine of both sides of the equation (31) is taken, the equation: sin θ (t) = sin (2πM / T) t is obtained. Therefore, the waveform shaping for this basic sine wave sin (2πM / T) t To obtain sin θ (t) that satisfies the equation (Equation 21).

のようなフーリエ級数として表すことができる。ここで、係数ｂ_ｉは、式（数３６）：

で表される。 Since sin θ (t) to be obtained is a periodic function of an odd function, in general, Expression (Equation 35):

Can be expressed as a Fourier series. Here, the coefficient b _i is an expression (Equation 36):

It is represented by

が得られる。ここで、ｋは任意の正の整数である。 Here, if the order of the harmonics is an odd number, the waveform at the time of increase and decrease of sin θ (t) can be made in synchronization with the basic sine wave sin (2πM / T) t, so that the rotation angle it is possible to reduce the deviation of the acceleration alpha _2. Therefore, in the equation (41), if the term of i = 1 (term of the basic sine wave) and the term of i = 2k + 1 (term of the harmonic having an odd order) are left and the other terms are eliminated, the waveform shaping is performed. Since frequency components other than the required order can be cut, the occurrence of angular acceleration can be suppressed as a result. At this time, the equation (Equation 37):

Is obtained. Here, k is an arbitrary positive integer.

Sin θ (t) expressed by the equation (Equation 37) satisfies the equation (Equation 17). Since the outer frame 15 from that M rotated around _{Y 0} axis between the period T, that sin [theta is expressed by Fourier series having a basic angular frequency of 2πM / T, sinθ (t) is equation (37 It is clear that the equation (Equation 17) holds even if it is not represented by (). Further, since cos θ is also expressed by a Fourier series having 2πM / T as a basic angular frequency, Expression (Expression 18) is satisfied, and further, Expression (Expression 12) is satisfied with respect to an arbitrary positive integer m. If the rotation speeds M and N are selected, the equation (Equation 19) is also established.

を満たす必要がある。 Also, since sin θ (t) expressed by the equation (Equation 37) needs to satisfy the equation (Equation 21), the coefficients b ₁ and b _{2k + 1} are expressed by the equation (Equation 38):

It is necessary to satisfy.

を満たす必要がある。 Further, in order to give rotation continuity, since sin θ (t) = 1 must be satisfied when t = (n−1) T + T / 4M, the coefficients b ₁ and b _{2k + 1} are expressed by the following equation (Equation 39):

It is necessary to satisfy.

になる。 From the above, when k is an odd number (k = 1, 3, 5,...), Sin θ (t) is the fundamental sine wave and the fundamental angular frequency of 3, 7, 11,.・ Harmonics with double angular frequency are superimposed. At this time, the equation (Equation 39) becomes the equation (Equation 40):

become.

で表される。 Therefore, from the equation (Equation 38) and the equation (Equation 40), the solution of the coefficients b ₁ and b _{2k + 1} is the equation (Equation 41):

It is represented by

になる。 On the other hand, when k is an even number (k = 2, 4, 6,...), Sin θ (t) is a fundamental sine wave and a fundamental angular frequency of 5, 9, 13,. A harmonic having a double angular frequency is superimposed. At this time, the equation (Equation 39) becomes the equation (Equation 42):

become.

で表される。 Therefore, from the equations (Equation 38) and (Equation 42), the solutions of the coefficients b ₁ and b _{2k + 1} are expressed by the equation (Equation 43):

It is represented by

  Therefore, Formula (Formula 11)-Formula (Formula 13) were derived.

Here, two cases are considered when k is an odd number and an even number, but in either case, two sets of solutions are obtained as solutions of the coefficients b ₁ and b _{2k + 1} . Here, either set of solutions may be selected, but if a solution whose absolute value of b _{2k + 1} is smaller than the absolute value of b ₁ is selected, the angular acceleration represented by the equation (Equation 10) It can reduce the size of the alpha _2.

Further, in order to reduce the size of the angular acceleration alpha ₂ represented by the equation (10) is preferably a low angular frequency of the harmonics of the sin [theta (t). Therefore, if k = 1 is set and a solution whose absolute value of b _{2k + 1} is smaller than the absolute value of b ₁ is selected, the magnitude of the angular acceleration α ₂ is minimized.

で表される。ここで、係数ｂ_１、ｂ_３は、式（数４５）：

で与えられる。 At this time, sin θ (t) is expressed by Equation (Equation 44):

It is represented by Here, the coefficients b ₁ and b ₃ are expressed by the equation (Equation 45):

Given in.

で表される。 Therefore, θ (t) that minimizes the magnitude of the angular acceleration α ₂ is expressed by the following equation (Equation 46):

It is represented by

<Preservation method of platelet preparation>
A method for preserving a platelet preparation according to an embodiment of the present invention will be described. The method for preserving the platelet preparation according to the present embodiment is performed in a predetermined temperature range using the three-dimensional clinostat 11.

In the present embodiment, the platelet preparation is not particularly limited as long as it can be used for blood transfusion, but in general, platelet rich plasma (PRP) which is plasma obtained by concentrating platelets from peripheral blood. It is synonymous. Usually, platelet preparations contain 0.2 × 10 ¹¹ platelets per unit in citrated plasma. In the present embodiment, the platelet preparation storage container is not particularly limited as long as it is a container that can stably store platelets. Of these materials, preferred) are preferred. In the present embodiment, the predetermined temperature range is not particularly limited as long as it is an optimal temperature range for storing the blood product, but generally, when the blood product is a platelet product, the optimal temperature range is: 22 ° C. ± 2 ° C. When the blood product is an erythrocyte product, the optimum temperature range is 4 ° C. ± 2 ° C. When the blood product is a plasma product, the optimum temperature range is −20 ° C. or lower. In this embodiment, since it is a case where a platelet formulation is preserve | saved, an optimal temperature range is 22 degreeC +/- 2 degreeC. This temperature region may be referred to as room temperature.

  The platelet preparation is enclosed in a sample enclosure 23 of at least one platelet preparation storage container 21. Thereafter, at least one platelet product storage container 21 is stored in the platelet product storage unit 12. Thereafter, the three-dimensional clinostat 11 is operated to rotate the platelet preparation container 12 biaxially. Thereby, since the platelet preparation storage container 21 can be rotated in three dimensions, the platelets in the platelet preparation can be stored while being uniformly stirred with a constant load in three dimensions.

  Therefore, if the platelet preparation storage method according to the present embodiment is used, the platelet preparation in the platelet preparation is rotated three-dimensionally and stirred uniformly with a constant load, whereby the platelet preparation is horizontally Compared with the case of shaking, the stress applied to the platelets can be reduced. Therefore, since the platelet preparation can be stored while suppressing platelet aggregation, the quality of the platelet preparation can be maintained and the storage period of the platelet preparation can be extended.

  In the present embodiment, the platelet product storage container 21 is held in the inner frame 14 before the platelet product storage container 21 is stored in the platelet product storage unit 12, but the present invention is not limited to this. Alternatively, after the platelet product storage container 21 is stored in the platelet product storage unit 12, the inner frame 14 may hold the platelet product storage unit 12.

  Further, in the present embodiment, the case where the platelet preparation storage container 21 is stored while being rotated using the three-dimensional clinostat 11 is described. However, the present invention is not limited to this, and three-dimensional or three-dimensional or more You may make it preserve | save, rotating the platelet formulation storage container 21 using the rotation apparatus which can be rotated in multiple dimensions.

  In the present embodiment, the case where the platelet preparation is stored using the three-dimensional clinostat 11 is described. However, the present invention is not limited to this, and other than platelets, for example, an anticoagulant, a storage solution, and the like. Other blood products other than platelet products such as erythrocyte product and plasma product, culture solution containing human, animal or plant cells, or artificial organs may be stored.

  Although the content of this embodiment is demonstrated in detail below using an Example and a comparative example, this embodiment is not limited to a following example.

<How to save>
Example 1
After blood of Japanese white rabbit (100 ml) was collected, centrifugation was performed at a centrifugal gravity of about 400 G for 10 minutes to collect platelet rich plasma (PRP), which was the supernatant excluding the precipitated red blood cells. . The collected PRP was stored in the storage member 22 installed in the three-dimensional clinostat 11 in the Opticell (manufactured by NUNC) and stored in a rotating state (three-dimensional rotation condition) at room temperature for 72 hours. . In addition, in a present Example, room temperature is an optimal temperature range for storing a platelet formulation, and means the range of 20 degreeC or more and 24 degrees C or less.

(Comparative Examples 1 and 2)
In Comparative Example 1, the same procedure as in Example 1 was performed except that the PRP storage condition was changed to a stationary state (stationary condition).
In Comparative Example 2, the PRP storage condition was changed to a horizontally shaken state (horizontal shaking condition: manufactured by BC-730 BIO CRAFFT), and the same as in Example 1 except that the storage was performed by two-dimensional movement. went.

<Evaluation method>
Platelet count, mean platelet volume (MPV), platelet distribution width (PDW), clot retraction rate, hypoosmotic shock recovery rate between PRP immediately after blood collection and PRP after storage by the methods of each Example and Comparative Example The platelet aggregation reaction was measured and evaluated by the following method.

(Platelet count, mean platelet volume (MPV), platelet distribution width (PDW))
Platelet count, MPV, and PDW were measured for PRP immediately after blood collection and PRP after storage by the methods of Examples and Comparative Examples using an automatic blood cell counter (F-820, Sysmex Corporation). For the platelet count and MPV, 11 samples were prepared and the average value was obtained. PDW prepared eight samples and calculated the average value.

(Clot retraction rate)
A thrombin solution of 5 units / mL was added to 0.2 mL of PRP immediately after blood collection and PRP after storage by the methods of each Example and Comparative Example, and after mixing well, the mixture was allowed to stand at 37 degrees for 30 minutes. Thereafter, the weight of serum produced by clot retraction was measured with an electronic balance, and the clot retraction rate was determined based on the following formula (A). The clot retraction rate was determined by averaging 6 samples.
Clot retraction rate = serum weight / initial PRP weight × 100 (%) Formula (A)

(Low osmotic pressure recovery rate)
The low osmotic pressure shock recovery rate was measured by the PRP immediately after blood collection and the change in turbidity of the PRP after storage by the methods of Examples and Comparative Examples. The change in turbidity was measured by adding 0.2 mL of distilled water to 0.4 mL of PRP and measuring the change in turbidity for 5 minutes measured using CAF-100 (manufactured by JASCO Corporation). The change in turbidity immediately after adding distilled water was taken as 100%, and the rate of turbidity recovery after 5 minutes was taken as the low osmotic pressure shock recovery rate. The hypoosmotic shock recovery rate was determined by averaging 8 samples.

(Platelet aggregation reaction)
The platelet agglutination reaction was performed by adding adenosine diphosphate (ADP) at a concentration of 10 μM to the PRP immediately after blood collection and 0.4 mL of PRP after being stored by the methods of each Example and Comparative Example, and the agglutination reaction was carried out by the turbidimetric method. It was measured. For the platelet aggregation reaction, 8 samples were prepared and the average value was obtained.

<Evaluation results of Example 1 and Comparative Examples 1 and 2>
Platelet count, MPV, PDW, clot retraction rate, hypoosmotic shock recovery rate, and platelet aggregation reaction of PRP immediately after blood collection and PRP after storage by the method of Example 1 and Comparative Examples 1 and 2, respectively The evaluation results are shown in FIGS. FIG. 4 is a graph showing the evaluation results of the platelet count between PRP immediately after blood collection and PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2, and FIG. FIG. 6 is a diagram showing the evaluation results of MPV with PRP after storage by the method of Example 1 and Comparative Examples 1 and 2, and FIG. 6 shows PRP immediately after blood collection, Example 1, and Comparative Example 1, FIG. 7 is a diagram showing the results of PDW evaluation with PRP after storage by the method of FIG. 2, and FIG. 7 shows PRP immediately after blood collection and PRP after storage by the methods of Example 1 and Comparative Examples 1 and 2 FIG. 8 is a graph showing the evaluation results of the blood clot retraction rate, and FIG. 8 shows the low osmotic pressure shock recovery rate between the PRP immediately after blood collection and the PRP stored by the method of Example 1 and Comparative Examples 1 and 2 FIG. 9 shows the PRP immediately after blood collection, Example 1, and Comparative Example 1, Is a graph showing evaluation results of the platelet aggregation reaction with PRP after storage at ways.

(Platelet count)
As shown in FIG. 4, Example 1 was closer to the PRP platelet count immediately after blood collection than Comparative Examples 1 and 2. A decrease in platelet count is an indicator of platelet damage. When PRP is stored at room temperature for 72 hours under static conditions, the platelet count tends to decrease compared to immediately after blood collection (see Comparative Example 1), so the platelet preparation is stored under static conditions. If this happens, there is a possibility that platelet destruction has occurred. When PRP was stored at room temperature for 72 hours under horizontal shaking conditions or three-dimensional rotation conditions, a tendency to suppress the decrease in the number of platelets was observed compared to the case where platelet preparations were stored under stationary conditions (implementation) See Example 1 and Comparative Example 2). In particular, when PRP was stored under three-dimensional rotation conditions, the platelet count was maintained at a value almost similar to the platelet count of PRP immediately after blood collection, and a significantly higher platelet count was maintained compared to the horizontal infiltration condition. (See Example 1). Therefore, it was found that when PRP was stored under a three-dimensional rotation condition, platelet destruction tends to be suppressed.

(MPV and PDW)
As shown in FIG. 5 and FIG. 6, the MPV and PDW of PRP after storing using any of the storage methods of Example 1 and Comparative Examples 1 and 2 are almost similar to MPV and PDW of PRP immediately after blood collection And there was little change. MPV and PDW serve as indices indicating changes in platelet morphology. Therefore, it was found that even when PRP was stored for 72 hours at room temperature under the three-dimensional rotation condition instead of the stationary condition and the horizontal shaking condition, the platelet morphology could be maintained in the same manner as the conventional storage method.

(Clot retraction rate)
As shown in FIG. 7, the PRP clot retraction rate after storage using any of the storage methods of Example 1 and Comparative Examples 1 and 2 is lower than the PRP clot retraction rate immediately after blood collection. However, the clot retraction rate was higher in Example 1 than in Comparative Examples 1 and 2. Clot retraction is a reaction in which clotted blood (clot) reduces volume and produces serum. Since the reaction that produces this serum involves platelets, the clot retraction rate is an indicator of platelet function. Even if PRP is stored at room temperature for 72 hours under static conditions, horizontal shaking conditions, or three-dimensional rotation conditions, the platelet function is reduced, so there is a clear difference due to differences in the storage method of PRP. Absent. However, since the blood clot retraction rate was higher in Example 1 than in Comparative Examples 1 and 2, the case where PRP was stored under the three-dimensional rotation condition rather than the stationary condition and the horizontal shaking condition was better. It was found that the decrease in platelet function tends to be suppressed.

(Low osmotic pressure recovery rate)
As shown in FIG. 8, compared with Comparative Examples 1 and 2, Example 1 was a value almost similar to the low osmotic pressure shock recovery rate of PRP immediately after blood collection, and was almost the same value. When platelets are exposed to low osmotic pressure, the volume of platelets once swells, but then the platelet volume decreases because the platelets contract. Therefore, it was found that if the PRP was stored for 72 hours at room temperature under the three-dimensional rotation condition instead of the stationary condition and the horizontal shaking condition, the function almost the same as that of the PRP immediately after blood collection could be maintained.

(Platelet aggregation reaction)
As shown in FIG. 9, the platelet aggregation reaction of PRP after storage using any of the storage methods of Example 1 and Comparative Examples 1 and 2 was lower than the platelet aggregation reaction of PRP immediately after blood collection. However, the platelet aggregation reaction of PRP after storage was higher in Example 1 than in Comparative Examples 1 and 2. Since platelet aggregation is an important reaction for hemostasis, the platelet aggregation reaction is an indicator of platelet function. It can be said that when PRP is stored under a stationary condition or a horizontal shaking condition, functions related to the platelet aggregation reaction are reduced as compared with PRP immediately after blood collection. Since the platelet aggregation reaction was higher in Example 1 than in Comparative Examples 1 and 2, platelet platelets were more preserved when PRP was stored under three-dimensional rotation conditions than with stationary conditions and horizontal shaking conditions. It can be said that it is preferable as a storage method for PRP because the deterioration of the function related to the agglutination reaction is further suppressed. In particular, in the PRP in which the PRP is stored under the three-dimensional rotation condition, the platelet aggregation reaction with respect to ADP is significantly higher than the horizontal shaking condition, and it can be said that the function related to the platelet aggregation reaction is maintained. Therefore, it was found that if the PRP was stored for 72 hours at room temperature under the three-dimensional rotation condition instead of the stationary condition and the horizontal shaking condition, the function almost the same as that of the PRP immediately after blood collection could be maintained.

(Example 2)
PRP was collected in the same manner as in Example 1 except that human blood (5 adult males in their 20s to 40s) was used. The collected PRP was stored in Opticell (manufactured by NUNC) for 7 days in a state of being accommodated in the accommodating member 22 installed in the three-dimensional clinostat 11 and rotating (three-dimensional rotation condition).

(Comparative Example 3)
In Comparative Example 3, the PRP storage condition was changed to a horizontally shaken state (horizontal shaking condition: manufactured by BC-730 BIO CRAFFT), and the same as in Example 2 except that the PRP was stored by two-dimensional movement. went.

<Evaluation results of Example 2 and Comparative Example 3>
FIG. 10 is a diagram showing the evaluation results of the platelet count between PRP immediately after blood collection and PRP after storage by the method of Example 2 and Comparative Example 3, and FIG. 11 shows PRP immediately after blood collection and Example FIG. 12 is a diagram showing the evaluation results of MPV with PRP after storage by the method of 2 and Comparative Example 3, and FIG. 12 shows PRP immediately after blood collection and PRP after storage by the method of Example 2 and Comparative Example 3 FIG. 13 is a diagram showing evaluation results of platelet aggregation reaction between PRP immediately after blood collection and PRP stored by the method of Example 2 and Comparative Example 3. .

(Platelet count)
As shown in FIG. 10, in Example 2, it was almost constant even after being stored at room temperature for 7 days, whereas in Comparative Example 3, there was a tendency that the platelet count gradually decreased with the elapsed days. Further, a significant difference in the platelet count was observed between the platelet count of Example 2 and the platelet count of Comparative Example 3 after 7 days. Therefore, it was found that when PRP is stored under three-dimensional rotation conditions, platelet destruction is suppressed.

(MPV and PDW)
As shown in FIG. 11 and FIG. 12, the PRP MPV and PDW after storage using any of the storage methods of Example 2 and Comparative Example 3 tended to increase after 2 days of storage. There was no significant difference between Example 2 and Comparative Example 3.

(Platelet aggregation reaction)
As shown in FIG. 13, the platelet aggregation reaction of PRP after storage using any of the storage methods of Example 2 and Comparative Example 3 tended to decrease with the storage days, and was almost recognized after 7 days of storage. I can't. On the other hand, the reduction rate of the platelet aggregation reaction of PRP of Comparative Example 3 is larger than the reduction rate of the platelet aggregation reaction of Example 2 PRP, and in particular, the platelet aggregation reaction of Example 2 PRP after 5 days of storage and the comparative example Significant differences were found between the three PRP platelet aggregation reactions. Since the platelet aggregation reaction of Example 2 was higher than that of Comparative Example 3, the PRP stored under a three-dimensional rotation condition tends to exhibit a higher aggregation reaction than the horizontal shaking condition. Since it is recognized, it can be said that it is preferable as a PRP storage method. In particular, in the PRP in which the PRP is stored under the three-dimensional rotation condition, the platelet aggregation reaction with respect to ADP is significantly higher than the horizontal shaking condition, and it can be said that the function related to the platelet aggregation reaction is maintained. Therefore, it was found that, by storing the PRP under the three-dimensional rotation condition instead of the horizontal shaking condition, it is possible to suppress a decrease in the function with respect to the PRP immediately after blood collection.

  From these results, when storing the platelet preparation, it can be confirmed that the platelet preparation stored in three dimensions rather than the two dimensions tends to retain platelets immediately after blood collection. Therefore, by storing the platelet preparation while rotating in three dimensions, the agitation motion when storing the platelet preparation can be minimized, and the burden on the platelet can be minimized. It can be said that it is expensive. For this reason, it can be said that storing the platelet preparation while rotating in three dimensions contributes to suppressing the decrease in the function of the platelet and maintaining the function of the platelet.

  Therefore, it is possible to store the platelet preparation while rotating the platelet preparation in three dimensions and maintaining the number of platelets and suppressing the aggregation of the platelets by minimizing the stirring motion when storing the platelet preparation. Therefore, it is possible to contribute to the extension of the storage period of the platelet preparation while maintaining the quality of the platelet preparation. As a result, it is possible to contribute to providing a high-quality platelet preparation.

10 Platelet preparation storage device 11 Three-dimensional clinostat (rotating device)
12 Platelet preparation housing part 13 Holding part 14 Inner frame (first rotating body)
15 Outer frame (second rotating body)
16 Leg 17 17 1st motor (1st drive part)
18 Second motor (second drive unit)
DESCRIPTION OF SYMBOLS 19 Base 21 Platelet preparation storage container 23 Sample enclosure part 24 Partition part 26 Lid 31 Rotation angle detector 32 Rotation angle detector 33 Motor control part (motor controller)
34 orbit generator 35 control device

Claims (6)

A platelet product storage section for storing a platelet product storage container for storing the platelet product;
A rotating device that rotates the platelet preparation container by n-axis (n is an integer of 2 or more);
A platelet preparation storage device characterized by comprising: In claim 1,
The platelet preparation storage device, wherein the rotating device generates a pseudo-weightless state. In claim 1 or 2,
The rotating device is
A holding part for holding the platelet preparation container;
A first rotating body connected to the holding unit and holding the holding unit;
A first drive section for rotating the first rotating body around the first rotation axis;
A second rotator for rotatably holding the first rotator about a first rotation axis;
A leg that holds the second rotating body rotatably about a second rotating shaft having an axial direction in a direction different from the axial direction of the first rotating shaft;
A second drive unit that rotates the second rotating body around the second rotation axis;
A platelet preparation storage device characterized by comprising: In any one of Claims 1 thru | or 3,
The platelet product storage device, wherein the platelet product storage part has a partition part for partitioning a plurality of the platelet product storage containers inside the platelet product storage part.   A method for preserving a platelet preparation, comprising storing the platelet preparation while rotating a platelet preparation storage container for storing the platelet preparation n-axis (n is an integer of 2 or more). In claim 5,
A method for preserving a platelet preparation, which comprises rotating the platelet preparation storage container n-axis to generate a pseudo weightless state.

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