JP2015124180A5 - - Google Patents
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- JP2015124180A5 JP2015124180A5 JP2013269637A JP2013269637A JP2015124180A5 JP 2015124180 A5 JP2015124180 A5 JP 2015124180A5 JP 2013269637 A JP2013269637 A JP 2013269637A JP 2013269637 A JP2013269637 A JP 2013269637A JP 2015124180 A5 JP2015124180 A5 JP 2015124180A5
- Authority
- JP
- Japan
- Prior art keywords
- skin
- cells
- heat shock
- ultraviolet rays
- heat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000003491 Skin Anatomy 0.000 description 11
- 210000004027 cells Anatomy 0.000 description 7
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 6
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 6
- 210000001519 tissues Anatomy 0.000 description 5
- 206010042496 Sunburn Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000002633 protecting Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 241000238582 Artemia Species 0.000 description 1
- 229940041514 Candida albicans extract Drugs 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 101710006484 HSPA14 Proteins 0.000 description 1
- 101710013836 HSPD1 Proteins 0.000 description 1
- 102100003681 HSPD1 Human genes 0.000 description 1
- WZUVPPKBWHMQCE-VYIIXAMBSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@@]2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-VYIIXAMBSA-N 0.000 description 1
- 101710022862 Hsp60A Proteins 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 210000004927 Skin cells Anatomy 0.000 description 1
- 206010027699 Skin injury Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 101700057149 hsp60 Proteins 0.000 description 1
- 239000000399 hydroalcoholic extract Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000004625 regulation of water loss via skin Effects 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Description
ヒートショックプロテインは、細胞が熱や紫外線等のストレスにさらされた時に発現し、細胞を保護するタンパク質の一群であり、その分子量によりそれぞれの分子に名前がつけられており、例えばＨＳＰ６０、７０、９０はそれぞれ分子量６０、７０、９０kDaのタンパク質を指す。ヒートショックプロテインに関しては、ヒートショックプロテインおよびヒートショックプロテイン因子を産生させる酵母抽出液を含む皮膚外用剤により、紫外線、熱などの外的刺激から皮膚を保護し、損傷を受けた皮膚を修復し、改善することが報告されている（例えば、特許文献１参照）。
また、紫外線や乾燥、熱、低温などの外的環境ストレスに対して、皮膚細胞の修復や保護効果を有することにより、皮膚のバリヤー機能を向上させ、肌老化や肌あれを改善、予防する効果を有する抗皮膚ストレス用化粧料としては、海洋プランクトン、アルテミア（Ａｒｔｅｍｉａ Ｓａｌｉｎａ 和名：ホウネンエビ）の孵化直前の耐久卵から水抽出した活性エキス成分を配合した化粧料（例えば、特許文献２参照）、紅藻の含水アルコール抽出物により、ストレス蛋白質の合成を誘発できる技術が知られている（例えば、特許文献３参照）。
Heat shock proteins are a group of proteins that are expressed when cells are exposed to stress such as heat and ultraviolet rays and protect the cells. Each molecule is named according to its molecular weight. For example, HSP60, 70, 90 refers to proteins with molecular weights of 60, 70, and 90 kDa, respectively. With regard to heat shock protein, skin external preparations containing yeast extract that produces heat shock protein and heat shock protein factor protect the skin from external stimuli such as ultraviolet rays and heat, repair damaged skin, Improvement has been reported (for example, see Patent Document 1).
In addition, it has the effect of improving and preventing skin aging and rough skin by improving skin barrier function by repairing and protecting skin cells against external environmental stresses such as ultraviolet rays, drying, heat, and low temperature. As an anti-skin-stress cosmetic material having a composition, a cosmetic material containing an active extract component extracted from a durable egg immediately before hatching of marine plankton and artemia (Artemia S alina Japanese name: Hoonen shrimp) (see, for example, Patent Document 2) ), And a technique capable of inducing the synthesis of stress protein by a hydroalcoholic extract of red algae (see, for example, Patent Document 3).
＜試験例２＞
（皮膚損傷予防作用試験）
ヒートショックプロテインの皮膚に対する有用性の一つとして、紫外線照射による障害を低減する作用が知られている。そこで紫外線により損傷を生じた皮膚で観察される日焼け細胞（サンバーンセル：ｓｕｎｂｕｒｎ ｃｅｌｌ）の出現数を指標として、皮膚損傷予防作用を調べた。
ヒト新鮮皮膚組織（BIOPREDIC International社製）を用意し、皮膚表面に実施例１〜４及び比較例１〜２をそれぞれ皮膚組織表面に１ｃｍ²あたり10μｌを塗布した。その後、皮膚表面に紫外線（ＵＶＢ）を１００ｍＪ／ｃｍ²となるように照射した。組織を１０％ホルマリンで固定後、組織切片標本を作製し、ヘマトキシリン・エオジン染色した。組織切片標本の１mm長当りのサンバーンセル数を数え、無塗布部位でのサンバーンセル数を100として実施例及び比較例塗布部位のサンバーンセル出現率（％）を算出し表４に示した。
<Test Example 2>
(Skin injury prevention test)
As one of the usefulness of heat shock protein to the skin, the effect of reducing damage caused by ultraviolet irradiation is known. Therefore sunburn cells observed in the skin caused damage by UV: as (San burn cell sunbur n c ell) index number of occurrences were examined skin damage prevention effect.
Fresh human skin tissue (manufactured by BIOPREDIC International) was prepared, and Examples 1 to 4 and Comparative Examples 1 to 2 were applied to the skin surface at a rate of 10 μl per 1 cm 2 on the skin tissue surface. Thereafter, the skin surface was irradiated with ultraviolet rays (UVB) so as to be 100 mJ / cm <sup2>. After fixing the tissue with 10% formalin, a tissue section specimen was prepared and stained with hematoxylin and eosin. The number of sunburn cells per 1 mm length of the tissue section sample was counted, and the number of sunburn cells at the non-application site was defined as 100. The appearance rate (%) of the sunburn cell at the application site of Examples and Comparative Examples was calculated and shown in Table 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013269637A JP6289896B2 (en) | 2013-12-26 | 2013-12-26 | Topical skin preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013269637A JP6289896B2 (en) | 2013-12-26 | 2013-12-26 | Topical skin preparation |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015124180A JP2015124180A (en) | 2015-07-06 |
| JP2015124180A5 true JP2015124180A5 (en) | 2016-12-15 |
| JP6289896B2 JP6289896B2 (en) | 2018-03-07 |
Family
ID=53535148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013269637A Active JP6289896B2 (en) | 2013-12-26 | 2013-12-26 | Topical skin preparation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6289896B2 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5143354B2 (en) * | 2005-11-22 | 2013-02-13 | 日本精化株式会社 | Oligomer ester and cosmetics and skin external preparations containing these |
| JP5113371B2 (en) * | 2006-11-22 | 2013-01-09 | 日本精化株式会社 | Hydrolyzate of water-soluble polysaccharide derived from white jellyfish, antioxidant containing the same, cosmetic or skin external preparation, food |
| JP2009126791A (en) * | 2007-11-20 | 2009-06-11 | Kracie Home Products Kk | Oil-in-polyhydric alcohol type emulsified cosmetic |
-
2013
- 2013-12-26 JP JP2013269637A patent/JP6289896B2/en active Active
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