JP2015093860A - Delayed allergy inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a delayed allergy inhibitor showing excellent inhibitory effect on delayed allergy.SOLUTION: A delayed allergy inhibitor comprises a carotenoid compound represented by the formula (I) as an active ingredient, where R-Rindependently represent a hydrogen atom or Calkanoyl.

Description

  The present invention relates to a delayed allergy inhibitor effective for the prevention and treatment of delayed allergies classified as type IV allergies.

  Allergic disease is a general term for diseases caused by excessive immune reaction, and is mainly classified from type I to type V.

  Among these, type IV allergy is characteristic in that other allergies are related to humoral immunity involving antibodies, whereas they are related to cellular immunity involving T cells and macrophages. More specifically, when T cells are sensitized by reacting with an antigen, various physiologically active substances such as macrophage activators are released to cause tissue damage. Since type IV allergy takes time to collect, proliferate and activate lymphocytes, it takes about 24 to 48 hours from contact with an antigen to onset compared to other acute allergies. Also called allergy.

  Examples of delayed allergies include contact dermatitis, tuberculin reaction, rejection of various organ transplants, insulin-dependent diabetes, Shugren's syndrome, chronic granulomatous disease, Guillain-Barre syndrome, and the like. Atopic dermatitis is said to be a combined type of type I allergy and type IV allergy.

  Conventionally, steroidal drugs have been mainly used as inhibitors of delayed allergy from the viewpoint of suppressing inflammation. However, since there are concerns about side effects in steroidal drugs, there are demands for drugs with fewer side effects. For example, Non-Patent Document 1 reports that delayed allergy is suppressed by fucoxanthin, which is a carotenoid derived from brown algae.

Sakai, S. Et al., Biosci. Biotechnol. Biochem. , 75 (5), pp. 1013-1015 (2011)

  As described above, it is known that fucoxanthin, which is a natural carotenoid compound, exhibits an inhibitory effect on delayed allergy in addition to steroidal drugs. However, since allergic diseases have become a problem in recent years, there is a demand for better delayed allergy inhibitors.

  Then, an object of this invention is to provide the delayed allergy inhibitor which shows the outstanding inhibitory effect with respect to delayed allergy.

  The inventors of the present invention have made extensive studies to solve the above problems. As a result, it was found that a specific carotenoid compound exhibits a delayed allergy suppressing effect superior to the fucoxanthin described above, and the present invention has been completed.

  The delayed allergy inhibitor according to the present invention is characterized by containing a carotenoid compound represented by the following formula (I) as an active ingredient.

[Wherein R ^{1 to} R ³ independently represent a hydrogen atom or a C _1-18 alkanoyl group]
As the carotenoid compound (I), those having a three-dimensional structure of the following formula (I ^1),

[Wherein R ^{1 to} R ³ are as defined above]
R ¹ and R ² are preferably hydrogen atoms, and R ³ is preferably an acetyl group.

  As the dosage form of the delayed allergy inhibitor according to the present invention, an external preparation is preferred. The external preparation is suitable for the prevention and treatment of contact dermatitis and atopic dermatitis among delayed allergies.

  The delayed allergy inhibitor according to the present invention can effectively suppress delayed allergy. In particular, among the onset mechanism of delayed allergy, the migration of eosinophils to eotaxin and the aggregation of eosinophils can be suppressed to reduce inflammation and the like. In addition, the delayed allergy inhibitor according to the present invention is a carotenoid compound, and it can be said that there are few side effects and high safety compared to steroidal drugs. Therefore, the delayed allergy inhibitor according to the present invention is very useful as a substance that can effectively prevent and treat delayed allergy.

FIG. 1 is a graph for comparing the effects of peridinin according to the present invention, fucoxanthin as a comparative example, and cortisol as a corticosteroid on inflammation (ear thickness) caused by delayed allergy. FIG. 2 is a graph for comparing the effects of peridinin, fucoxanthin and cortisol on the suppression of eosinophil production at sites of inflammation due to delayed allergy. FIG. 3 is a graph for comparing the effects of peridinin, fucoxanthin and cortisol on the inhibition of eosinophil production in peripheral blood due to delayed allergy. FIG. 4 is a graph comparing the effects of peridinin, fucoxanthin and cortisol on the suppression of the production of eotaxin, a ligand for eosinophil chemokine receptors, at sites of inflammation due to delayed allergy. FIG. 5 is a graph for comparing the inhibitory effects of peridinin, fucoxanthin and cortisol on eosinophil chemotaxis by eotaxin.

  The delayed allergy-suppressing agent according to the present invention is characterized by containing the carotenoid compound (I) as an active ingredient.

The “C _1-18 alkanoyl group” which is R ^{1 to} R ³ of the carotenoid compound (I) according to the present invention refers to a formyl group or a C _1-17 alkyl-carbonyl group, where “C _1-17 alkyl group” "Means a linear or branched monovalent saturated hydrocarbon group having 1 to 17 carbon atoms. Examples of the C _2-18 alkanoyl group include acetyl group, ethylcarbonyl group, n-propylcarbonyl group, isopropylcarbonyl group, n-butylcarbonyl group, pivaloyl group, n-hexylcarbonyl group, n-octylcarbonyl group, n -Nonylcarbonyl group, 6-methyloctylcarbonyl group, 7-methyloctylcarbonyl group, n-decylcarbonyl group, 8-methylnonylcarbonyl group, n-undecylcarbonyl group, 9-methyldecylcarbonyl group, n-dodecylcarbonyl Group, 10-methylundecylcarbonyl group, n-tridecylcarbonyl group, 11-methyldodecylcarbonyl group, n-tetradecylcarbonyl group, n-pentadecylcarbonyl group, n-hexadecylcarbonyl group, n-heptadecylcarbonyl Group etc. That. The C _1-18 alkanoyl group is preferably a C _1-8 alkanoyl group or a C _9-18 alkanoyl group, more preferably a C _1-6 alkanoyl group, still more preferably a C _1-4 alkanoyl group, and a C _1-2 alkanoyl group. A group is further preferred, and an acetyl group is particularly preferred.

As the carotenoid compound (I) according to the present invention, those represented by the following formula (I ¹ ) are suitable. The three-dimensional structure of the following carotenoid compound (I ¹ ) is that of the dinoflagellate Symbiodinium sp. This corresponds to the three-dimensional structure of peridinin, which is a natural carotenoid compound isolated from the OTCL2A strain.

R ¹ and R ² are preferably hydrogen atoms, and R ³ is preferably a C _1-18 alkanoyl group. The carotenoid compound (I ¹ ) in which R ¹ and R ² are hydrogen atoms and R ³ is an acetyl group is the above-described peridinin.

  The excellent delayed allergy-suppressing effect of the above preferred compounds has been demonstrated by the experimental results described below.

  The compounds of the present invention, particularly the above-mentioned peridinin, can be obtained from Symbiodinium sp. It can be isolated and purified from dinoflagellates such as OTCL2A strain.

  Among the dinoflagellates that produce the carotenoid compound (I) according to the present invention, Symbiodinium sp. Is symbiotic and has a symbiotic relationship with marine invertebrates. Examples of marine animals that are hosts include foraminifers, radiolarians, flatfish intestines, jellyfishes, corals, and bivalves. Therefore, in order to isolate the carotenoid compound (I) from nature, the dinoflagellate Symbiodinium sp. Is isolated from the marine animals and cultured, and the cultured dinoflagellate is homogenized and then extracted.

  The extraction solvent may be appropriately selected. For example, alcohol solvents such as methanol and ethanol; ketone solvents such as acetone and methyl ethyl ketone; halogenated hydrocarbon solvents such as dichloromethane and chloroform; aromatics such as benzene and toluene Hydrocarbon solvents; ester solvents such as methyl acetate and ethyl acetate; ether solvents such as diethyl ether and tetrahydrofuran; amide solvents such as dimethylformamide and dimethylacetamide; among these organic solvents having a concentration of 60 v / v% or more Mention may be made of a mixed solvent of a water-miscible organic solvent and water.

  What is necessary is just to adjust extraction conditions suitably. For example, although extraction can be performed at normal temperature, in order to improve extraction efficiency, you may heat to about 30-60 degreeC depending on temperature. Moreover, in order to improve extraction efficiency, it is preferable to stir at the time of extraction. Alternatively, after adding the extraction solvent to the dinoflagellate, the dinoflagellate may be pulverized in the extraction solvent. Further, the extraction time is not particularly limited, but can be, for example, about 1 hour or more and 10 days or less.

  After the extraction operation, the active ingredient should be identified by chromatography such as thin layer chromatography, HPLC, preparative chromatography, etc. while measuring the inhibitory effect of delayed allergy or referring to physical property data of known compounds. That's fine.

As described above, the carotenoid compound (I) may be isolated from dinoflagellate or may be produced by derivatizing the isolated compound. For example, the carotenoid compound (I) in which any one or more of R ^{1 to} R ³ is a C _1-18 alkanoyl group can be produced by esterifying a predetermined hydroxyl group. Such esterification can be carried out by a method known to those skilled in the art using an acid corresponding to the C _1-18 alkanoyl group, an activated ester compound or an acid chloride compound thereof, and, if necessary, a condensing agent. However, R ³ of peridinin, which is a natural compound in the carotenoid compound (I), is an acetyl group.

  The carotenoid compound (I) according to the present invention can be used as an active ingredient of a delayed allergy inhibitor. In the present invention, “suppression” includes both the concepts of suppression of onset of delayed allergy, ie, “prevention”, and reduction of onset delayed allergy, ie, “treatment”. Therefore, the delayed allergy inhibitor of the present invention may be used prophylactically before the onset of delayed allergy, or is used to treat or inhibit the progression of symptoms while symptoms are relatively mild. Or may be used to relieve severe symptoms.

  The dosage form of the delayed allergy suppressing agent of the present invention is not particularly limited, and includes, for example, external preparations such as ointments, lotions, solutions, suspensions, patches, liniments, poultices; troches and suppositories Preparations for mucous membranes such as: injections; powders, granules, capsules, pills, tablets, extracts, elixirs, suspensions, emulsions, tinctures, syrups, etc. Among delayed allergies, general contact dermatitis and atopic dermatitis are preferred skin preparations because they are skin diseases.

  The delayed allergy inhibitor of the present invention includes a carotenoid compound (I), which is an active ingredient, and a base material, excipient, coloring agent, lubricant, corrigent, emulsifier, emulsifier, and the like depending on its dosage form. A sticking agent, a wetting agent, a stabilizer, a preservative, a solvent, a solubilizing agent, a suspending agent, an antioxidant, an adjuvant, a buffering agent, a pH adjusting agent, a sweetener, a fragrance and the like can be blended.

  The dose of the carotenoid compound (I) according to the present invention may be appropriately adjusted according to the patient's symptoms, age, sex, area of the affected area, whether it is preventive use or therapeutic use, etc. For example, when administered to humans, 0.01 mg or more and 10 mg or less per application by application to the skin, etc., 0.1 mg / kg body weight / day or more and 50 mg / kg body weight / day or less by oral administration It can be. The number of administrations per day may be appropriately adjusted according to the severity of symptoms, particularly the degree of itchiness, and can be, for example, about once / day or more and three times / day or less.

  In addition, the delayed allergy-suppressing agent according to the present invention can be used as daily necessities.

  For example, basic cosmetics such as milky lotion, skin lotion, cream, facial cleanser, gel, cosmetic liquid, pack and mask; makeup cosmetics such as foundations, lipsticks, white powder and dusting powders, blushers, eyebrows, and beautiful nails; Hair washing cosmetics such as shampoos and rinses; hair styling agents, hair treatment agents, hair restorers, permanent wave agents, hair dyes, hair bleaches and other hair cosmetics; perfume and cologne aromatic products; soaps and liquid body washing Cosmetics such as cosmetics, suncare products, hand care products, deodorant cosmetics, body cosmetics such as depigmenting agents and hair removal agents; and oral cosmetics such as dentifrices and mouth fresheners. That is, you may mix | blend the carotenoid compound (I) based on this invention as a component of the said cosmetics in order to suppress delayed allergy.

  In addition, bathing is effective for skin diseases such as atopic dermatitis in terms of cleansing the skin, sweating, applying humidity, and increasing body temperature. Therefore, the delayed allergy inhibitor according to the present invention is used as a bath agent. That is, the carotenoid compound (I) according to the present invention may be added to hot water.

  In this case, the carotenoid compound (I) according to the present invention may be used directly in hot water, or the solution is dipped in a porous bag made of nonwoven fabric and dried, It may be used while being soaked in and squeezed out. Moreover, you may mix | blend with inorganic salts, such as sodium chloride, sodium hydrogencarbonate, sodium carbonate, sodium sulfate, potassium chloride, potassium carbonate which are hot spring components.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited by the following examples, but may be appropriately modified within a range that can meet the purpose described above and below. Of course, it is possible to implement them, and they are all included in the technical scope of the present invention.

Production Example 1: Isolation of peridinin Symbiodinium sp. The OTCL2A strain was isolated from Tridacna crocea. The OTCL2A strain was cultured in ES medium at 25 ° C. for 40 days. 82.0 g of OTCL2A strain cells obtained from 132 L of the culture solution were frozen and homogenizer (Janke & Kunkel GmbH, Co. KGIKA-Labortechnik, Germany, “ULTRATURRAX T25”) in 70 v / v% ethanol water (150 mL). After leaving still at 4 degreeC for 3 days, it centrifuged. The supernatant and the precipitate were separated, and extracted again from the precipitate twice with 70 v / v% ethanol water (150 mL each) under the same conditions as described above. The extracts were combined and concentrated under reduced pressure. The residue was suspended in water (100 mL) and extracted three times with ethyl acetate (200 mL each). The extracts were combined and concentrated under reduced pressure to obtain an ethyl acetate soluble fraction (903 mg). A part (676 mg) of the fraction was subjected to silica gel column chromatography (silica gel: “Silica Gel 60” manufactured by Nacalai Tesque, 60 mL, eluent: dichloromethane / methanol = 99/1 → 98/2 → 96/4 → 92/8. , 120 mL each). A part (199.2 mg) of the fraction eluted with dichloromethane / methanol = 96/4 and 92/8 (199.2 mg) was charged to an ODS column (manufactured by Nacalai Tesque, “Cosmosil 75C18-OPN”, 19 mL). And eluted with 80 v / v% aqueous methanol (40 mL) and 85 v / v% aqueous methanol (80 mL). Peridinine was purified (24.7 mg) by HPLC under the following conditions from a fraction (98.8 mg) eluted with 85 v / v% aqueous methanol.
HPLC conditions Column: “Cosmosil 5C18-AR-II” manufactured by Nacalai Tesque, 20 mmφ × 250 mm
Eluent: 85% acetonitrile water Flow rate: 6.0 mL / min

Comparative production example 1: Isolation of fucoxanthin A brown alga Petalonia fascia (54.52 g) was frozen and extracted four times with methanol (500 mL each). The obtained extracts were combined and concentrated under reduced pressure. The residue was dissolved in 90 v / v% aqueous methanol (100 mL), and then impurities were extracted twice with hexane (100 mL each). The remaining 90 v / v% methanol aqueous layer was concentrated. The residue was added to water (100 mL) and then extracted twice with ethyl acetate (100 mL each). The ethyl acetate soluble fraction (602.1 mg) was charged onto a silica gel column (Nacalai Tesque, “Silica Gel 60”, 12 mL) and eluted with hexane / ethyl acetate = 1/1 (170 mL). The fifth fraction (32.4 mg) was charged to an ODS column (manufactured by Nacalai Tesque, “Cosmosil 75C18-OPN”, 10 mL), and 85 v / v% methanol water (40 mL) and 90 v / v% methanol water (30 mL). ). The third fraction eluted with 90 v / v% aqueous methanol contained fucoxanthin (12.8 mg).

Test Example 1: Effect on delayed allergy BALB / c female mice were purchased from Clea Japan Co., Ltd., and 8-10 weeks old mice were used for experiments. First, in order to prevent proliferation of immunosuppressive cells, 150 mg / kg of cyclophosphamide aqueous solution was subcutaneously injected. Two weeks later, the abdomen was shaved, and a 7 mass% solution in which 2,4,6-trinitrochlorobenzene (picryl chloride, hereinafter abbreviated as “PCl”) was dissolved in a mixed solution of ethanol / acetone = 3/1. Immunization was performed by applying (0.05 mL) to the shaved part. Two weeks after sensitization, 10 μg of peridinin according to the present invention, fucoxanthin as a comparative example, or cortisol as a corticosteroid was applied to the ear, or 50 μg of peridinin or fucoxanthin was intraperitoneally administered. Three hours after the above application or administration, a 1% by mass solution (0.02 mL) in which PCI was dissolved in a mixture of acetone / olive oil = 1/4 was applied to the earlobe to perform antigen exposure. The thickness of the ears was measured with a dial gauge (manufactured by Ozaki Seisakusho) at 0, 4, 24 and 48 hours after antigen exposure. For comparison, the same experiment was conducted in an example in which no drug was administered and in an example in which no antigen exposure was performed by sensitization alone. Each experiment was performed 10 times, and the average value was calculated. In addition, the obtained results were subjected to analysis of variance and subjected to Turkey-Kramer's post hoc test. The results are shown in FIG. In FIG. 1, “*” indicates a case where there is a significant difference with respect to an example where p <0.01 and no drug is administered.

  As shown in FIG. 1, fucoxanthin could not suppress delayed allergy either by application or intraperitoneal administration. In contrast, cortisol, an adrenocortical hormone, strongly suppressed delayed allergy, and the suppression rates at 24 hours and 48 hours after antigen exposure were 22.4% and 18.2%, respectively. In addition, peridinin according to the present invention is not a steroid hormone, but can significantly suppress delayed allergy, and the inhibition rate at 24 hours and 48 hours after antigen exposure is 8 when applied to the ear. 0.9% and 9.2%, respectively, 12.7% and 11.7% when administered intraperitoneally.

Test Example 2: Inhibitory Effect of Eosinophils at Inflamed Sites Eosinophils are known to increase in the inflamed part of delayed allergy. Therefore, eosinophils were used as an index of the effect of peridinin on delayed allergy according to the present invention.

  Specifically, in the same manner as in Test Example 1, sensitization with PCI, application administration of peridinin, fucoxanthin or cortisol or intraperitoneal injection, and antigen exposure were performed. 48 hours after the antigen exposure, tissues were collected from the earlobe, and the samples were stained with a hematoxylin / Giemsa solution. Counted. Counting was performed at at least 10 places, the average value was calculated in the same manner as in Test Example 1, and a Turkey-Kramer's significance test was performed. The results are shown in FIG. In FIG. 2, “*” indicates a case where there is a significant difference from an example in which p <0.01 and no drug is administered.

  As shown in FIG. 2, fucoxanthin could not suppress the increase in eosinophils at the inflamed site by either application or intraperitoneal administration. On the other hand, when cortisol, which is a corticosteroid, was applied, the number of eosinophils was suppressed by 68.5% compared to the case where no drug was administered. In addition, peridinin according to the present invention suppressed the number of eosinophils at the inflamed site by 79.9% when applied to the ear and 60.3% when administered intraperitoneally.

Test Example 3: Inhibitory Effect of Eosinophils in Peripheral Blood In the same manner as in Test Example 1 described above, sensitization with PCI, application administration of peridinin, fucoxanthin or cortisol, intraperitoneal injection administration, and antigen exposure were performed. Forty-eight hours after antigen exposure, blood was collected from the retroorbital plexus, spread on a glass slide, and stained with Giemsa solution. In the obtained sample, the proportion of eosinophils in at least 200 leukocytes was determined. The measurement was performed on 10 or more samples, the average value was calculated in the same manner as in Test Example 1, and a Turkey-Kramer's significance test was performed. The results are shown in FIG. In FIG. 3, “*” indicates a case where there is a significant difference from an example in which p <0.01 and no drug is administered.

  As shown in FIG. 3, fucoxanthin could not suppress the increase in eosinophils in peripheral blood, either by application or intraperitoneal administration. In contrast, when cortisol, an adrenocortical hormone, was applied, the number of eosinophils in peripheral blood was suppressed by 83.1% compared to the case where no drug was administered. In addition, peridinin according to the present invention suppressed the number of eosinophils in peripheral blood by 82.2% when applied to the ear and 78.4% when administered intraperitoneally.

  Separately, the concentration of IL-5 in the serum of the peripheral blood of mice treated with peridinin was measured, but its production was not suppressed. Since IL-5 selectively induces the proliferation of eosinophils, the formation of eosinophils by peridinin is suppressed by eotaxin, which strongly induces eosinophil migration at the site of inflammation. It is thought that it is suppressed.

Test Example 4: Inhibitory effect of eotaxin In the same manner as in Test Example 1, sensitization with PCl, application of peridinin, fucoxanthin or cortisol or intraperitoneal injection, and antigen exposure were performed. 48 hours after antigen exposure, ears were collected from each mouse, and PBS containing 0.1 v / v% surfactant (Tween 20) was added at 100 μL per 10 mg ear sample, and zirconia beads having a diameter of 5 mm were added, Homogenization was performed for 2 minutes at 30 Hz using a mixer mill (Retsch, “MM300”). The resulting homogenate was centrifuged at 12,000 rpm for 10 minutes. The supernatant was stored at −80 ° C. until measurement of eotaxin. The concentration of eotaxin in the supernatant was measured with a measurement kit (manufactured by Ray Biotech, “Mouse eotaxin ELISA kit”). In addition, the obtained results were subjected to analysis of variance and subjected to Turkey-Kramer's post hoc test. In addition, the detection limit of eotaxin under the above conditions is 0.01 pg per 1 mg of tissue. The results are shown in FIG. In FIG. 4, “*” indicates a case where there is a significant difference with respect to an example where no drug is administered at p <0.01, and “**” indicates a case where there is a significant difference at p <0.05.

  As shown in the results of FIG. 4, antigen exposure with PCI promoted the production of eotaxin, which is a ligand for eosinophil chemokine receptors. Such eotaxin production was not suppressed by application of fucoxanthin, but was slightly suppressed by intraperitoneal administration at a rate of 19.4%. On the other hand, peridinin according to the present invention had no inhibitory effect when administered intraperitoneally, but inhibition was observed at an inhibition rate of 30.6% when applied by application. This inhibition rate was comparable to the inhibition rate of 42.1% for cortisol.

  From the above results, it can be said that peridinin and fucoxanthin have different production suppression mechanisms for eotaxin, and thus different eotaxin production suppression mechanisms by activated endothelial cells, epithelial cells, macrophages and the like. Moreover, it is thought that peridinin and fucoxanthin have different skin permeability.

Test Example 5: Eotaxin inhibits eosinophil chemotaxis
Tominaga, A. Et al. Exp. Med. , 173, pp. According to 429-437 (1991), IL-5 transgenic mice (C3H / HeN-TgN (IL-5) -Imega) were generated and maintained in a specific pathogen-free (SPF) state. From 8-15 week old IL-5 transgenic mice, Watanabe, Y. et al. Et al., DNA Cell Biol. , 20, pp. A composition enriched in eosinophils was obtained by a modification of the Percoll specific gravity gradient centrifugation method described in 189-202 (2001). The isotonic percoll solution was prepared using a 10 × solution of Krebs Ringer PBS (10 mM sodium phosphate buffer (pH 7.5) containing 154 mM sodium chloride, 6 mM potassium chloride and 1 mM magnesium chloride, hereinafter abbreviated as “KRP”). Prepared and diluted with KRP to 60 v / v%, 70 v / v% and 80 v / v%. After putting 2 mL of KRP suspension containing 20 to 50 × 10 ⁶ cells into a 15 mL conical polypropylene tube, 2.5 mL each of the above-mentioned percoll solutions was carefully laminated from the lowest concentration. The tube was centrifuged at 1000 xg for 20 minutes at room temperature. B lymphocytes and T lymphocytes in the 70-80 v / v% percoll fraction were bound to anti-B220 and anti-Thy1.2 binding beads (DYNAL AS, “Dynabeads”) using a permanent magnet By removing the beads, a fraction with increased purity of eosinophils was obtained. A part is separated from the fraction. Exp. Med. According to the method described in (1991), eosinophils were stained with peroxidase and the proportion thereof was measured. As a result, 93% of all cells were eosinophils.

4 × 10 ⁵ eosinophil cells obtained above were suspended in 300 μL of RPMI 1640 medium containing 0.1% by mass of BSA and placed in the upper well of a 24-well chemotaxis chamber (Kurabo) having a pore size of 5 μm. Added. To the lower chamber, 800 μL of RPMI 1640 medium containing 0.1% BSA and 20 ng / mL eotaxin derived from mouse was added. 1 μg or 3 μg of peridinin or fucoxanthin was then added to each transwell. The assay plate was incubated for 1 hour at 37 ° C. in a 5% CO ₂ atmosphere. After incubation, the number of cells that permeated the filter and moved to the lower chamber was counted. For comparison, the same experiment was performed when no drug was added and when eotaxin was not added. . In addition, the obtained results were subjected to analysis of variance and subjected to Turkey-Kramer's post hoc test. The results are shown in FIG. The result of FIG. 5 is represented by the number of cells in the range of 660 μm × 840 μm. Each group of experiments was performed in 6 wells, and the number of eosinophils in the above range of 3 cases was counted in each well. That is, the result of FIG. 5 is an average of 18 cases. In FIG. 5, “*” indicates a case where there is a significant difference at p <0.01.

  As shown in FIG. 5, chemotaxis of eosinophils to eotaxin was suppressed by 24.2% and 61.7% by 1 μg or 3 μg of fucoxanthin, respectively, but 1 μg or 3 μg of peridinin was further suppressed. By 57.4% and 72.8%, respectively. Thus, it was demonstrated that peridinin can more effectively suppress the chemotaxis of eosinophils to eotaxin than fucoxanthin.

Claims (5)

A delayed allergy inhibitor comprising a carotenoid compound represented by the following formula (I) as an active ingredient.

[Wherein R ^{1 to} R ³ independently represent a hydrogen atom or a C _1-18 alkanoyl group] The delayed allergy inhibitor according to claim 1, wherein the carotenoid compound has a three-dimensional structure represented by the following formula (I ¹ ).

[Wherein R ^{1 to} R ³ are as defined above] The delayed allergy inhibitor according to claim 1 or 2, wherein R ¹ and R ² are hydrogen atoms. The delayed allergy inhibitor according to any one of claims 1 to 3, wherein R ³ is an acetyl group.   It is an external preparation, The delayed allergy inhibitor in any one of Claims 1-4.