JP2015065870A - Medium information registration system, colony detection device, hygiene control system, and program - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a medium information registration system and the like which can prevent overdetection and non-detection to accurately perform colony detection and counting thereof.SOLUTION: A hygiene control system S includes a configuration comprising the steps of: (1) determining colony pixels on the basis of the color feature amount of respective pixels constituting a medium image of the media image data that is an imaging of a film type medium 60; (2) performing general colony detection processing on the basis of the determined colony pixels; (3) performing, when the detection condition of the general colony detection processing or the detection result thereof satisfies the predetermined conditions, special colony detection processing different from the general colony detection processing; and (4) registering colony information detected in each detection processing into the database as the medium information.

Description

  The present invention relates to a food hygiene management system and the like.

  In recent years, due to the growth of fast food and other food service industries and the increase in easy processed foods, the opportunity to eat food prepared by others has increased remarkably. On the other hand, when foods such as processed foods and processed foods are mixed with dangerous substances such as poisons or microorganisms, it directly affects the human body. When handling processed foods, not only the safety of raw materials but also the production There is a need for food safety that strictly controls the hygiene of the process and covers the entire production and processing of food.

  In particular, food poisoning incidents that have occurred in various places are the starting point, and it is a proposition to further improve the safety of foods. In addition to complying with the Food Sanitation Law, “HACCP (Hazard Analysis Critical) The number of companies introducing “Control Point System” and “FSSC (Food Safety System Certification) 22000” is also increasing.

  As a system for detecting microorganisms mixed in such processed foods, a system using a film-type medium and counting colonies generated in a specimen cultured in the film-type medium by an image is known (for example, Patent Document 1).

JP 2011-2112013 A

  However, in the system such as Patent Document 1 as described above, in order to accurately detect a colony and prevent its non-detection, detection sensitivity for various parameters and detection criteria for performing colony detection. However, if the detection sensitivity is increased for various parameters and detection criteria, over-detection that detects bubbles and other colonies as colonies may occur, and appropriate colony detection may not be possible. Many.

  The present invention has been made to solve the above-mentioned problems, and its purpose is to prevent over-detection and non-detection, and to detect and count colonies accurately, and a culture medium information registration system Is to provide etc.

  In order to solve the above-described problem, the culture medium information registration system and the like according to the present invention includes an acquisition unit that acquires, as culture medium image data, data of a culture medium imaged by imaging a culture medium in which a colony is cultured, Extraction means for extracting a color feature amount relating to the color of a pixel constituting the medium image of the obtained medium image data, and a colony pixel constituting a colony in the medium image based on the color feature amount of the extracted pixel A first detection unit for performing a first colony detection process for identifying and detecting the colonies cultured in the medium based on the identified colony pixels and counting the detected colonies; and the detection unit A determination unit that determines whether at least one of the detection condition and the detection result satisfies a predetermined determination condition When the detection condition, the detection result, or both are determined to have a predetermined determination condition, colony detection different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means for executing the process as a second colony detection process, and information on colonies detected in either the first colony detection process or the second colony detection process is registered in the database as medium information. And a registration unit for performing the above-described registration.

  The medium information registration system or the like according to the present invention determines that the predetermined condition is satisfied when, for example, the colony is not detected when the general-purpose colony detection process is executed, and the colony detection sensitivity is high sensitivity. If the special colony detection process with a higher colony detection sensitivity than the general-purpose colony detection process is performed, the parameters and detection criteria are set so that Over-detection and non-detection can be prevented.

  Therefore, the hygiene management system and the like of the present embodiment can accurately execute detection and counting of colonies, and register medium information including information on accurately detected colonies and the number thereof in the database. it can.

It is a lineblock diagram showing the composition of one embodiment of the hygiene management system concerning the present invention. It is an example of the film type culture medium used for the sanitary management system of one Embodiment. It is an example of the work instruction used for the sanitary management system of one embodiment. It is a block diagram which shows the structure of the portable communication terminal device used for the sanitary management system of one Embodiment. It is a block diagram which shows the structure of the server apparatus used for the sanitary management system of one Embodiment. It is a figure for demonstrating the principle of operation of the colony detection determination part in the server apparatus of one Embodiment. It is a figure for demonstrating the special colony detection process of the colony detection determination part in the server apparatus of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the portable communication terminal device of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the server apparatus of one Embodiment.

  Hereinafter, embodiments according to the present invention will be described with reference to the drawings. In the following embodiment, the medium information registration system, colony detection according to the present invention is applied to a hygiene management system using a film-type medium in a food production line or an inspection line (hereinafter referred to as “work line”). It is embodiment at the time of applying a device, a program, and a hygiene management system. However, the present invention is not limited to the following embodiments within the scope including the technical idea.

[1] Hygiene management system First, the hygiene management system S of this embodiment will be described with reference to FIG. In addition, FIG. 1 is a block diagram which shows the structure of the sanitary management system S of this embodiment.

  The sanitary management system S of the present embodiment extracts a food during or after manufacture as a specimen in a work line 80 having a manufacturing process for manufacturing a processed or processed food or an inspection process for inspecting the food, Information relating to the film-type medium 60 (hereinafter referred to as “medium information”) such as the culture status when cultivated bacteria (colonies) such as common bacteria or E. coli generated in the specimen in the film-type medium 60 are cultured. This is a system for managing data in association with information relating to the work from which the specimen is extracted (hereinafter referred to as “work information”), and for managing the hygienic state in the manufacture or inspection of food.

In particular, the sanitary management system S of this embodiment is
(1) Based on the color feature amount of each pixel constituting the medium image of the medium image data obtained by imaging the film-type medium 60 (hereinafter referred to as “medium image data”), each pixel is an image of the colony. Identify the pixels that make up (hereinafter referred to as “colony pixels”),
(2) Colony detection processing (hereinafter referred to as “general colony detection processing” or “first colony detection processing”) that detects colonies based on the identified colony pixels and counts the number of the detected colonies. Run,
(3) When it is determined that the detection condition or detection result of the general-purpose colony detection process has a predetermined condition, unlike the general-purpose colony detection process, the colony detection process with higher colony detection sensitivity than the general-purpose colony detection process (Hereinafter referred to as “special colony detection process” or “second colony detection process”),
(4) It has the structure which registers the information regarding the colony detected in each detection process of any one of a general-purpose colony detection process or a special colony detection process into a database as culture-medium information.

  In order to realize such a configuration, the sanitary management system S of the present embodiment, as shown in FIG. 1, includes a plurality of portable communication terminal devices 10 used when registering culture medium information regarding each film-type culture medium 60. In addition, the administrator terminal device 20 that manages the sanitary management system S, the network 30, and the database 400, and in conjunction with the portable communication terminal device 10 or the administrator terminal device 20, registration management of medium information is performed. And a server device 40 that executes various types of processing.

The work information of this embodiment includes, in addition to various types of information related to the manufacturing process or inspection process, food extracted as a specimen from the manufacturing process or inspection process and information related to the culture of the specimen, and is unique to each work. Identification information (hereinafter referred to as “work ID”) 71 is used to be registered in the database 400. Specifically, each work information includes
(1) work ID 71;
(2) Identification information (hereinafter referred to as “line ID”) of the work line 80 from which the sample is to be extracted or, if the work line 80 has a plurality of processes, the line ID and process identification information (hereinafter referred to as “line ID”). , Referred to as “process ID”) 71b;
(3) Product name of food as a sample, identification information of lot (hereinafter referred to as “lot ID”), lot name,
(4) work date and time indicating the work start time and end time;
(5) worker identification information (hereinafter referred to as “worker ID (employee ID)”) and worker name;
Is included. Each piece of work information is registered in the database 400 for each type and for each work.

  The portable communication terminal device 10 is, for example, a digital camera having a communication function, a tablet-type information terminal device, a smartphone or a portable telephone, or the like, or an imaging function such as a still image or a recording function (hereinafter simply referred to as “camera function”). And a communication terminal device portable by an operator.

  In particular, the portable communication terminal device 10 can generate a culture medium image data by imaging the film-type culture medium 60 using a camera function, and can generate an instruction sheet image data by imaging the work instruction sheet 70. It has a configuration. And the portable communication terminal device 10 can register the work ID 71, the medium image data of the film-type medium 60, and the metadata of the medium image data as medium information in the database 400 of the server device 40. It has a configuration.

  On the other hand, when the portable communication terminal device 10 transmits the culture medium information to the server device 40, BLUETOOTH (registered trademark), wireless LAN (WLAN: Wireless Local Area Network), or wireless PAN (WPAN: Wireless Personal Area Network). The data is transmitted to the server device 40 directly or via the access point 50 using a communication standard for short-range wireless communication or the like, or is transmitted to the server device 40 using a public telephone line network via a mobile base station (not shown). It has a configuration. The portable communication terminal device 10 has a browser function constructed by a markup language such as XML (extensible Markup Language), and executes an operation input instruction and an operation confirmation by the operator using the browser function. The culture medium information is transmitted to the server device 40 via the browser function.

  The administrator terminal device 20 is an information communication terminal device such as a tablet information terminal device, a smartphone, a personal computer, or a workstation.

  The administrator terminal device 20 manages administrator identification information (hereinafter referred to as “manager ID”), the administrator password, and the worker ID, manages access authority to the server device 40, and is portable. It functions as a control device capable of performing management of the terminal ID of the communication terminal device 10, correction of registered medium information, and other management.

  The administrator terminal device 20 has a browser function built in a markup language, like the portable communication terminal device 10, and uses the browser function to exchange data with the server device 40 and to generate a report. It has a configuration capable of executing browsing and the like.

  Note that the administrator terminal device 20 is configured so that, in the same lot, when each operation is interrupted due to a failure of a machine that executes a process or the like, when a registration error of medium information or the like occurs in the operator, medium information or other information It has the structure which can correct.

  The network 30 includes, for example, a public telephone line network (hereinafter referred to as “long-distance communication network”) including a mobile phone network, an IP (Internet Protocol) network such as a short-distance wireless network, or both of which are interconnected. Has been. However, the configuration of the network 30 is not limited to this.

  The server device 40 is a server device used in conjunction with the portable communication terminal device 10 or the administrator terminal device 20 to execute data processing of medium information and registration thereof.

  Specifically, the server device 40 uses a general-purpose colony detection process, which is a general-purpose detection process, for the colonies cultured in the film-type medium 60 from the medium image data obtained by imaging the film-type medium 60 and the number thereof. It has the structure to detect.

  In the general colony detection process, the server device 40 is a condition (hereinafter referred to as “determination condition”) in which detection conditions such as the type of specimen or fungus and detection results such as the number of colonies detected are determined in advance. ), For example, one or more of the parameters used when detecting a colony, its detection criteria, and the colony detection method execute a special colony detection process different from the general-purpose colony detection process The colony cultured in the film-type medium 60 and the number of the colonies are re-detected.

  By having such a configuration, in the sanitary management system S of the present embodiment, for example, when a general colony detection process is executed and a colony is not detected, it is determined that a predetermined condition is satisfied. If a special colony detection process that uses a detection method with a higher colony detection sensitivity than the general colony detection process is performed, the undetected state is detected. It is possible to prevent overdetection and non-detection of colonies.

  Therefore, in the sanitary management system S of the present embodiment, colony detection and counting can be accurately performed, and medium information including information regarding the accurately detected colonies and the number thereof is registered in the database. Can be done.

  Further, in the sanitary management system S of the present embodiment, it is possible to save resources as compared with the case where various data analyzes and provisions are performed using physical resources such as paper media, and the culture medium. By registering the culture medium information in the database 400, registration mistakes can be remarkably reduced, and management of difficult culture media can be facilitated. Therefore, resource saving can be achieved without consuming wasteful culture media.

[2] Film Type Medium Next, the film type medium 60 of the present embodiment will be described with reference to FIG. FIG. 2 is an example of a film-type medium 60 used in this embodiment.

  The film-type medium 60 used in the present embodiment is a medium for culturing bacteria generated in food as a specimen detected from each step of the work line 80 using a film or sheet-like dry medium. For example, the film-type medium 60 is used as a medium for culturing general live bacteria, coliforms, and Staphylococcus aureus. In addition, you may use as a culture medium which culture | cultivates various bacteria, substances, or microorganisms, such as mold | fungi, yeast, a Listeria bacterium, a microorganism for water quality, lactic acid bacteria, and a protein.

  The film-type medium 60 includes, for example, a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG. (Hereinafter referred to as “circular frame”) 62, a culture layer 63 for culturing bacteria provided in the frame, a cover sheet 64 covering the culture layer 63, and a base sheet 61. Medium ID66.

  For example, the culture medium ID 66 is formed of a barcode such as a two-dimensional barcode or alphanumeric characters, and is imaged together when the film-type culture medium 60 is imaged, such as a barcode recognition function or an OCR (Optical Character Recognition) function. It is acquired in the portable communication terminal device 10 or the server device 40 using the image analysis function.

  The base material sheet 61 is not particularly limited as long as it is a film-like or sheet-like base material, and for example, a plastic film or paper can be used. Preferred examples of plastic films include resin films such as polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polymethacrylate, polymethyl methacrylate, polymethyl acrylate, polyester, and polycarbonate. Preferred examples include polyethylene terephthalate and polypropylene synthetic paper. Note that the polypropylene-based synthetic paper is a film synthetic paper whose main raw material is polypropylene.

  The film-type culture medium 60 has an IC tag in which a culture medium ID 66 is stored. The short-range wireless communication interface 140 (for example, an IC tag reader) included in the portable communication terminal apparatus 10 causes the medium to be connected to the portable communication terminal apparatus 10. ID66 may be acquired.

[3] Work instruction sheet Next, the work instruction sheet 70 of this embodiment will be described with reference to FIG. FIG. 3 is an example of a work instruction sheet 70 used in the present embodiment.

  The work instruction sheet 70 of the present embodiment includes, for each food group manufactured or inspected under the same conditions, that is, for each lot, work names and contents of one or more manufacturing processes or inspection processes, and work lines 80 to be used. It is an instruction document that describes the name of the food (processed food) to be manufactured or inspected, the quantity, and other information.

  Further, the work instruction sheet 70 of the present embodiment has an instruction sheet ID 71a formed by printing or other methods on the upper right and a plurality of process IDs 71b formed by printing or other methods on a predetermined area. . The instruction sheet ID 71a and each process ID 71b are used as the work ID 71, and are formed of, for example, a barcode such as a two-dimensional barcode or alphanumeric characters. The instruction ID 71a and each process ID 71b are imaged in the same manner as the film-type medium 60 when the registration of the film-type medium 60 is executed, and analyzed by the portable communication terminal device 10 or the server device 40. Be recognized.

  The work instruction sheet 70 has an IC tag in which the work ID 71 of the instruction sheet ID 71a or the process ID 71b is stored, like the film type culture medium 60, and has an interface (short distance) that the portable communication terminal device 10 such as a tag reader has. The portable communication terminal device 10 may acquire the work ID 71 by the wireless communication interface 140). In the following description, unless otherwise specified, the work ID 71 indicates an instruction ID 71a or a process ID 71b.

[4] Portable Communication Terminal Device Next, the configuration of the portable communication terminal device 10 of the present embodiment will be described using FIG. FIG. 4 is a block diagram showing the configuration of the portable communication terminal device 10 of the present embodiment.

  The portable communication terminal device 10 of the present embodiment has a data storage unit 100 having a memory function used when various programs are executed, an imaging function, image data of the film-type medium 60, and other image data. In addition to the image data generation unit 110 that generates the media information, the media information is registered in the server device 40 after the initial registration and the post-cultivation registration immediately after the culture in conjunction with the server device 40 (hereinafter referred to as “medium information registration processing”). And an application control unit 120 that executes processing.

  The portable communication terminal device 10 includes a network communication unit 130 that communicates with the server device 40 and other communication devices, a short-range wireless communication interface 140 that exchanges data with an IC tag and other communication interfaces, Controls the current position detection unit 150 that detects the current position, the display unit 160, the display control unit 161 that controls the display unit 160, the operation unit 170 for inputting user operations, the timer 180, and the entire apparatus. A portable terminal management control unit 190.

  And the portable communication terminal device 10 has various necessary members such as a microphone, a speaker, and an e-mail transmission / reception function when having a mail function such as a telephone function and e-mail. Further, the above-described units are connected to each other by a bus 11 to exchange data.

  The data storage unit 100 includes an application storage unit 101 that stores various application programs, an image data storage unit 102 that stores image data generated by being captured by the image data generation unit 110, and portable communication. A program relating to management and control of the terminal device 10, and a ROM / RAM 103 that is used as a work area during the execution of each program and stores data used in each process executed by the portable communication terminal device 10; have.

  In particular, the application storage unit 101 includes an application program (hereinafter referred to as “application”) that is executed by the application control unit 120 in conjunction with the image data generation unit 110, the operation unit 170, the display control unit 161, and the image data storage unit 102. .) Is recorded. The application storage unit 101 also stores a browser program for realizing the above browsing function.

  In the image data storage unit 102, the imaged medium image data and instruction sheet image data, the image ID for managing each image data, various metadata corresponding to each image data such as the imaging time, and the work ID 71 The medium ID 66 and various flag information are stored in association with each other. The image ID is arbitrary identification information that is appropriately given in each portable communication terminal device 10.

  The image data generation unit 110 includes an optical system, a CCDI sensor (Charge Coupled Device Image Sensor) that converts an optical image input from the optical system into an electrical signal, and image data based on the electrical signal generated by the CCDI sensor. And a generating unit that generates

  In particular, when the work instruction sheet 70 is imaged, the image data generation unit 110 converts the imaged work instruction sheet 70 into an image and generates instruction sheet image data. The image data generation unit 110 images the film-type medium 60 that has been imaged when the bacteria generated in the specimen are cultured or the film-type medium 60 that has been cultured is imaged. 60 pieces of medium image data are generated as medium image data.

  The application control part 120 implement | achieves culture-medium information registration process with a predetermined application. In particular, the application control unit 120 executes various control programs for controlling each unit of the portable communication terminal device 10 using the culture medium registration application stored in the application storage unit 101, and the network communication unit 130, the display control unit 161, and Various processes are executed in conjunction with or under control of the operation unit 170.

  The application control unit 120 executes the medium registration application recorded in the application storage unit 101, controls the image data generation unit 110 to acquire the medium image data of the film-type medium 60 captured and the image data storage unit 102. To remember.

  Then, the application control unit 120 acquires the medium ID 66 from the imaged medium image data, and together with the acquired medium ID 66, the image data storage unit displays the medium image data, the metadata of the medium image data, and various flag information. 102. Note that the application control unit 120 may execute barcode recognition or OCR recognition in conjunction with another communication device or the database 400 via a communication line.

  In addition, the application control unit 120 performs initial registration for registering the culture medium information in the server device 40 at the start of the culture of the specimen after imaging of the culture medium image data or at a predetermined timing, and registers the culture medium information after the start of the culture of the specimen. Perform registration after incubation. In particular, the application control unit 120 identifies whether the registration is initial registration or post-cultivation registration based on the operator's instruction, and includes information (flag information) indicating either registration in the culture medium information in the server device 40. Send.

  The application control unit 120 acquires the current time from the timer 180 when the film-type medium 60 is imaged, and the image data storage unit as the metadata of the medium image data together with a predetermined image ID using the current time as the imaging time. 102. When the medium image data is transmitted to the server device 40, it is transmitted together.

  In addition, the application control unit 120, when the culture medium ID 66 and the work ID 71 that is the instruction ID 71a and the process ID 71b are stored in the IC tag, the operation unit 170, the display control unit 161, and the short-range wireless communication interface The medium ID 66 and the work ID 71 are acquired in conjunction with 140.

  When the server device 40 recognizes the medium ID 66 and the work ID 71 from the medium image data and the instruction sheet image data, the application control unit 120 transmits instruction sheet image data in addition to the medium image data as the medium information.

  Furthermore, the application control unit 120 may be realized as a function when the CPU (central processing unit) constituting the mobile terminal management control unit 190 executes an application in terms of implementation.

  The network communication unit 130 establishes a communication line with the server device 40 connected to the network 30 under the control of the application control unit 120 and the mobile terminal management control unit 190, and exchanges various data such as culture medium image data. Do.

  The short-range wireless communication interface 140 performs short-range wireless communication using an IC tag under the control of the application control unit 120 and the mobile terminal management control unit 190.

  The current position detection unit 150 recognizes the position of GPS (Global Positioning System) hygiene via the network 30 under the control of the application control unit 120 or the mobile terminal management control unit 190, and a satellite signal transmitted from the GPS satellite (GPS signal) is detected.

  Then, the current position detection unit 150 calculates (that is, detects) coordinate values indicated by the latitude and longitude of the current position of the portable communication terminal device 10 based on the detected GPS signal. In addition, the current position detection unit 150 provides the calculated coordinate value to the application control unit 120 as position information.

  In addition, when the portable communication terminal device 10 has a telephone function or a short-range wireless function, the portable communication terminal device 10 is based on the direction and the radio field intensity received by the telephone base station or the like for the radio wave used for the telephone or the short-range radio. The current position of the portable communication terminal device 10 may be calculated (detected).

  The display unit 160 has an image display area of a predetermined size (for example, 5 inches, W480 × H960 pixels), is configured by a panel of liquid crystal elements or EL (Electro Luminescence) elements, and is generated by the display control unit 161. A predetermined image is displayed based on the display data. In particular, in this embodiment, the display unit 160 is linked with the operation unit 170 when the culture medium registration application is being executed, and the work instruction sheet 70 and the film type imaged by various displays and images. An image with the culture medium 60 is displayed.

  The display control unit 161 generates drawing data necessary to cause the display unit 160 to draw a predetermined image under the control of the application control unit 120 or the mobile terminal management control unit 190, and the generated drawing data is displayed on the display unit. 160 is output.

  The operation unit 170 includes various confirmation buttons, operation buttons for inputting operation commands, a number of keys such as a numeric keypad, and a touch sensor provided on the display unit 160, and is used when performing each operation. It has become. Specifically, the operation unit 170 is used when performing operations for executing the various processes described above when the culture medium registration application is activated.

  In the present embodiment, the operation unit 170 can also be used when manually inputting the culture medium ID 66 directly.

  The timer 180 provides the application control unit 120 with the date and time when the image data generation unit 110 images the film-type medium.

  The portable terminal management control unit 190 is mainly configured by a central processing unit (CPU) and includes various input / output ports such as a key input port and a display control port, and the overall functions of the portable communication terminal device 10. In addition, overall functions for executing the information providing program are generally controlled.

[5] Server Device [5.1] Configuration Next, the configuration of the server device 40 of this embodiment will be described with reference to FIG. FIG. 5 is a configuration diagram showing the configuration of the server device 40 of the present embodiment.

  As shown in FIG. 5, the server device 40 of the present embodiment communicates with a database 400 in which various information such as work information and culture medium information is stored, and the portable communication terminal device 10 and the administrator terminal device 20. A communication control unit 410, a data processing unit 420 that executes various processes such as a culture medium information registration process, a server management control unit 430 that controls each unit of the server device 40, a ROM / RAM 440 that is used to control each unit, and a time And a timer 450 used for management. The above-described units are connected to each other by a bus 41, and data transfer is performed between the components.

  The communication control unit 410 is a predetermined network interface, establishes a communication line with the portable communication terminal device 10 or the administrator terminal device 20, and exchanges various data with the portable communication terminal device 10 or the administrator terminal device 20. I do.

  The database 400 includes an HDD, and includes a medium information database (hereinafter abbreviated as “medium information DB”) 401, a line information database (hereinafter abbreviated as “line information DB”) 402, and a lot information database (hereinafter referred to as “line information DB”). Lot information DB ”) 403 and a work management database (hereinafter abbreviated as“ work management DB ”) 404.

The medium information DB 401 is extracted for each process of each work line 80, and the medium information regarding the film-type medium 60 of the specimen acquired by being imaged by the portable communication terminal device 10 is registered for each work ID 71 at the time of registration. It is a database to which an ID is assigned and stored. For example, in the culture medium information DB 401,
(1) Medium ID
(2) Work ID 71
(3) Imaged medium image data (4) Image capture time (5) Other information including flag information (6) Number of colonies (7) Medium determination result (8) Size (number of pixels), barycentric position, each pixel Information on each detected colony such as feature amount (hereinafter referred to as “colony information”)
(9) Type of profile information (profile ID) used at the time of colony detection
Are recorded in association with each other.

  As the colony information, the number of colonies, and the medium determination in each medium information, the results determined by the data processing unit 420 are stored. The colony information, the number of colonies, and the medium determination in each medium information may be registered when the medium information is registered, or may be registered at a predetermined timing different from the registration timing of the medium information.

  When the specimen to be extracted is always extracted from the same process on the same work line 80 in the same lot, the work ID 71 may be a lot ID, and the specimen to be extracted is the same lot. However, when extracting from a different work line 80 or a different process, the line ID or the process ID 71b is obtained.

  Further, when a plurality of imaging times of medium information of the same film type culture medium 60 are registered (that is, when there are a plurality of culture examination times), in the same culture medium ID 66, a culture medium image for each culture examination time, Each medium information of imaging time, number of colonies, and medium determination is stored.

The line information DB 402 is a database in which line information regarding each work line 80 is stored for each line ID. For example, the line information DB 402 includes
(1) Line ID
(2) The process ID 71b of the process included in the work line 80 and the two types of data are recorded in association with each other. In addition, as a process classification, the name which identifies each process, such as material injection | pouring, mixing | blending, filling, boiling, packaging, and packaging, is used.

The lot information DB 403 is a database in which lot information regarding each lot is stored for each lot ID. For example, in the lot information DB 403,
(1) Lot ID
(2) Line ID of work line used for production or inspection of lots
(3) Lot work start time (4) Lot work end time (5) Work team of workers who perform lot work (6) Raw materials used for each product manufactured by lots (7) Delivery of raw materials Seven data of a trader or a place of origin are recorded in association with each other.

The work management DB 404 is a database that stores various types of information related to work contents. For example, the work management DB 404 includes
(1) Work ID 71
(2) Line ID of the work line from which the sample is to be extracted (or line ID and process ID 71b)
(3) Type of sample (food) (product name, lot ID and / or lot name)
(4) Inspection type (genus of bacteria detected as colonies such as general bacteria, Escherichia coli or Staphylococcus aureus) and color (coloring) information (RGB gradation value or range thereof) generated on the film-type medium 60
(5) Culture conditions (dilution rate, type of diluent, culture temperature and culture humidity)
(6) Culture start time (7) Culture time from the start of culture until a predetermined time has elapsed (8) Culture location (for example, the position of the incubator number and the number of steps in the incubator)
(9) Information (hereinafter simply referred to as “presence / absence of use of filter”) indicating whether or not a filter is used when a specimen is extracted in order to remove extra solid matter such as dust (hereinafter referred to as “filter information”). Called.)
(10) Work date and time (work start time and end time)
(11) Worker ID (and / or worker name)
11 data are recorded in association with each other.

  Note that only a single time (that is, an end time for ending the culture of the bacteria in the sample) may be set as the culture examination time, or after legal time from the culture start time or after a predetermined time has elapsed, for example, A plurality of times such as 24 hours from the start of culture, 48 hours, or 72 hours may be set.

The data processing unit 420 executes various types of data processing according to the application recorded in the ROM / RAM 440. In particular, the data processing unit 420 executes a predetermined program,
(1) Operation management of the communication control unit 410;
(2) Medium information registration processing executed in conjunction with each portable communication terminal device 10;
(3) Based on the culture medium ID 66 and the work ID 71 acquired during the culture medium information registration process, the colony detection standard (that is, profile) of the colony imaged in the culture medium image is specified, and the culture medium ID 66 and the culture medium ID 66 acquired during the culture medium information registration process A general colony detection process for detecting colonies imaged in the culture medium image based on the work ID 71, and performing a culture medium determination based on the count of the detected number of colonies and the number of colonies;
(4) In the general colony detection process, when a predetermined condition is satisfied, a special colony detection process for executing a colony detection process different from the general colony detection process and the inspection process;
(5) A medium determination process for performing medium determination based on the number of colonies detected by the general-purpose colony detection process or the special colony detection process,
(6) lot determination processing for determining a lot based on registered medium information;
(7) Report processing for outputting the determination result in a predetermined report format;
(8) Management and control of the database 400;
I do. Specifically, the data processing unit 420 executes a registration processing unit 421 that executes culture information registration processing, a colony detection determination unit 422 that executes profile identification processing, general-purpose colony detection processing, and special colony detection processing, and lot determination processing. A lot determination processing unit 424 for executing the report processing and a report processing unit 425 for executing the reporting processing.

  For example, the registration processing unit 421 of the present embodiment constitutes an acquisition unit and a registration unit according to the present invention, and the colony detection determination unit 422 includes an extraction unit, a first detection unit, a determination unit, and a first unit according to the present invention. 2 constitutes detection means. Furthermore, details of the registration processing unit 421, colony detection determination unit 422, lot determination processing unit 424, and report processing unit 425 of this embodiment will be described later.

  The server management control unit 430 is mainly configured by a central processing unit (CPU), and integrally controls each unit of the server device 40 by executing a program. Specifically, the server management control unit 430 performs login processing for each user and various other controls based on a login request from the portable communication terminal device 10 by a user operation.

  Various programs necessary for driving the server device 40 are recorded in the ROM / RAM 440. In particular, the ROM / RAM 440 includes information on determination criteria (hereinafter referred to as “medium determination criterion information”) used in performing each determination of medium determination (pass / fail determination), process determination, line determination, and lot determination of each film type medium 60. And each condition information such as a predetermined condition for executing the special colony detection process (hereinafter referred to as “condition information”) is stored. The ROM / RAM 440 is used as a work area during execution of each program.

  Note that the condition information includes information on the type and fungus type (colony type) of the specimen that executes the special colony detection process, and the threshold value for the number of colonies detected in the general-purpose colony detection process.

  The timer 450 is used for managing the time required when the medium information registration process and the lot determination process are executed.

[5.2] Registration Processing Unit Next, details of the registration processing unit 421 in the server device 40 of the present embodiment will be described.

  The registration processing unit 421 of the present embodiment performs a registration process for registering each medium information in the medium information DB 401 while being associated with the work ID 71 in conjunction with the portable communication terminal device 10. In particular, the registration processing unit 421 executes different medium information registration processing based on flag information indicating whether the registration is initial registration or post-culture registration included in the culture medium information.

(Initial registration)
When the flag information indicating the initial registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 executes a registration process based on the initial registration.

  Specifically, when the registration processing unit 421 acquires the culture medium information via the communication control unit 410, the registration processing unit 421 specifies the work ID 71. Further, the registration processing unit 421 searches the work management DB 404 based on the identified work ID 71, identifies the corresponding work information, and associates the acquired medium image data and imaging time with the identified work information. Register in the medium information DB 401.

  In particular, the registration processing unit 421 specifies the lot ID including the work line 80 from which the sample is extracted based on the acquired work ID 71, the line ID of the work line 80, the process ID 71b from which the sample is extracted, and the specification. The unique medium ID is assigned in association with the lot ID, line ID, and process ID 71b, and the medium image data and the imaging time included in the medium information are registered in the medium information DB 401.

  Note that the registration processing unit 421 registers the imaging time as the culture start time in the culture medium information DB 401. Further, as the culture start time, a preset time may be used instead of the imaging time on the assumption that the medium information registration process is executed at the time.

(Registration after culture)
When the flag information indicating post-cultivation registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 performs a registration process based on post-cultivation registration.

  Specifically, when the medium processing information is acquired via the communication control unit 410, the registration processing unit 421 specifies the work ID 71 included in the medium information, as in the initial registration. Then, the registration processing unit 421 searches the work management DB 404 based on the specified work ID 71, specifies the corresponding work information, associates the medium image data and the imaging time with the specified work information, and the medium information. Register in the DB 401.

  In particular, the registration processing unit 421 identifies the type of specimen and the type of bacteria to be examined in the work information, information on the type of specimen (hereinafter referred to as “specimen type information”) and information on the type of bacteria (hereinafter referred to as “ Medium information including "bacterial species information") is provided to the colony detection determination unit 422, and based on the medium information, a general-purpose colony detection process is executed, or special colonies are detected when predetermined conditions are satisfied. Execute the process.

  Then, the registration processing unit 421 causes the colony detection determination unit 422 to execute the medium determination on the colony detection determination unit 422 based on the number of colonies detected by the general colony detection process or the special colony detection process. The acquired colony information, the number of colonies, and the determination result are registered as medium information in the medium information DB 401 in association with the corresponding work ID 71.

  Note that the culture examination time in the culture medium information may use a preset time instead of the imaging time on the assumption that the registration process of the culture medium information is executed at the same time as the culture start time.

[5.3] Colony Detection Determination Unit [5.3.1] Operation Principle Next, the operation principle of the colony detection determination unit 422 of the present embodiment will be described with reference to FIG. In addition, FIG. 6 is a figure for demonstrating the operation | movement principle of the colony detection determination part 422 of this embodiment.

  The colony detection determination unit 422 detects the colonies cultured on the film-type medium 60 and the number thereof from the medium image data obtained by imaging the film-type medium 60 using a general-purpose colony detection process that is a general-purpose detection process. In addition, when the detection conditions used in the general colony detection process and the detection result detected by the general colony detection process have predetermined determination conditions, the general colony detection process is a parameter used when detecting a colony Then, a special colony detection process in which one or two or more of the detection standard and the colony detection method are different is executed, and re-detection of the colonies cultured in the film-type medium 60 and the number thereof is executed.

  Normally, when colonies are detected and counted from a medium image, depending on the occurrence of colonies, not only will the product stop shipping, but it will cause food poisoning in the worst case, thus preventing false detection. Is important.

  In addition, depending on the type of bacteria such as general bacteria or unprocessed food samples, a detection accuracy close to 100% is sufficient even if it is not 100%. In a special specimen such as food, it is necessary to determine that the film-type medium 60 is abnormal when at least one colony is detected. In this case, 100% detection accuracy is required. .

  However, when 100% detection accuracy is required for all bacterial species, there is a problem of overdetection that detects other than colonies such as bubbles or residues generated on the medium. Will be mistaken. In addition, in the case of E. coli or processed food, if undetected colonies occur, it will have a great influence as described above.

  For example, depending on the type of specimen and the type of fungus, as shown in FIG. 6A, the tone value of the color feature amount as a colony is high, and an object (for example, FIG. When the object α) of A) is formed on the film-type medium 60, it is an object that has not grown to a colony and that cannot be detected on the image even as a blurred area (for example, FIG. 6). The objects β1 to β3) in (A) tend not to be regarded as important.

  In other words, when there are clearly colonies on the image, it is not clear whether the colony is close to the color of the medium itself and the color feature value gradation value is low (light color). Tend not to detect. In FIG. 6A, objects β2 and β3 are also shown in an enlarged view.

  On the other hand, in the case of processed food or when hygiene problems occur when it is one of Escherichia coli, as shown in FIG. 6 (B), it is not clear whether it is a colony, If only the objects γ1 to γ3 having the same color feature amount as the object β in FIG. 6A are not detected as colonies in the general-purpose colony detection process, the objects γ1 to γ3 are also detected as colonies, In some cases, it may be desired to take out the portion and culture it again to determine the presence of colonies. In FIG. 6B, as in FIG. 6A, objects γ2 and γ3 are also shown in an enlarged view.

  Therefore, the colony detection determination unit 422 according to the present embodiment first executes a general-purpose colony detection process for detecting colonies in general, and determination conditions in which detection information, detection results, or both of the general-purpose colony detection processes are set in advance. In the case of the bacterial species or the type of specimen, it is estimated that colonies that are undetected or suspected to be over-detected are present on the image, and a special colony detection process with higher colony detection sensitivity than the general colony detection process is performed. It is supposed to run.

  Therefore, in the present embodiment, overdetection and non-detection can be prevented, and colony detection and counting can be accurately performed.

  Specifically, the colony detection determination unit 422 of the present embodiment first executes general-purpose colony detection processing, specimen type information and fungus information provided from the registration processing unit 421, and the general-purpose colony detection processing. The number of colonies detected by the above is compared with the condition information that is stored in the ROM / RAM 430 and that defines the specimen type and bacterial species for which the special colony detection process is executed and the threshold value for the number of colonies.

  Then, the colony detection determination unit 422, as a predetermined determination condition, when the specimen type information and the fungus species information match and the number of detected colonies is equal to or greater than the threshold specified in the condition information, A special colony detection process having higher colony detection sensitivity than the general colony detection process is executed.

  In addition, when the colony detection determination unit 422 executes the special colony detection process, the colony detection determination unit 422 provides the result to the registration processing unit 421 as the medium information, and performs the special colony detection process without providing the predetermined determination condition. When not executed, that is, when the specimen type information or the bacterial species information does not match or the number of detected colonies is smaller than the threshold specified in the condition information, the special colony detection process is not executed. In addition, the detection result by the general colony detection process is provided to the registration processing unit 421 as the medium information.

  In addition, about the determination conditions of this embodiment, it replaces with the point that each condition of sample classification information, fungus species information, and the number of colonies agrees, and any one or two conditions of sample classification information, fungus species information, and the number of colonies If they match, the colony detection determination unit 422 may execute a special colony detection process.

[5.3.2] General-purpose colony detection processing Next, details of the general-purpose colony detection processing in the colony detection determination unit 422 of the present embodiment will be described.

  The colony detection determination unit 422 uses the work information DB 404 or necessary information based on the work ID 71 specified by the registration processing unit 421 and, if necessary, information included in the culture medium information acquired by the registration processing unit 421. In such a case, information stored in another database is searched to obtain corresponding work information.

In addition, the colony detection determination unit 422 is based on conditions for detecting colonies such as the type of specimen, the bacterial species, the culture time, and the culture conditions included in the acquired work information.
(1) Type of characteristic amount related to color when colony pixels are detected from the medium image,
(2) Detection method for detecting colonies, and
(3) Parameters and detection criteria used in the detection method,
Among the plurality of profiles stored in the ROM / RAM 430, one profile is specified.

  The colony detection determination unit 422 sets various thresholds or parameters based on the specified profile, and determines the colony pixel detection method, the colony candidate detection method, and the colony specification method, Execute.

Specifically, the colony detection determination unit 422 performs general-purpose colony detection processing as follows:
(1) In order to detect pixels that are supposed to form an imaged colony from the medium image, a colony pixel having a condition set for a color-related feature amount is selected from the pixels that form the medium image. Detect
(2) Link colony pixels based on the set criteria, perform labeling to give ID, detect the connected colony pixel group as a colony candidate of one object,
(3) A colony candidate having the set standard is specified as a colony, and the number is counted.

  In particular, when the type of feature quantity is luminance or saturation, the colony detection determination unit 422 is a value obtained by executing a predetermined calculation based on the gradation value of each RGB sub-pixel of each pixel. And colony pixels are detected based on parameters defined in the profile. In addition, when the colony pixel detection method defined in the profile uses “binarization”, the colony detection determination unit 422 binarizes the feature amount defined in the profile with a threshold defined in the profile. Then, a pixel having one value is detected as a colony pixel.

  Furthermore, the colony detection determination unit 422 executes image filter processing including expansion processing and reduction processing for reducing image filter noise, connects adjacent colony pixels, performs labeling, and assigns an ID. Colony candidates are detected based on the connection method and its parameters.

  That is to say, the colony detection determination unit 422, regarding the connection of the colony pixels, based on the range that should be determined to be the same as the feature amount relating to the color of each colony pixel specified by the profile, the same colony candidate for the adjacent colony pixels Concatenate those determined to be.

  And the colony detection determination part 422 connects the colony pixel which adjoins, forms a colony pixel group, The pixel group which comprises the same colony pixel using the color characteristic for every colony pixel group, or the gravity center position on a culture medium image Connect them together.

  On the other hand, for each detected colony candidate, the colony detection determination unit 422 sets feature amounts such as color characteristics, roundness, shape, and size according to the feature amounts specified in the specified profile, and the set features Based on the amount, a likelihood indicating the likelihood of colony is calculated. And the colony detection determination part 422 specifies the colony candidate which has predetermined conditions, such as the calculated likelihood more than a threshold value, as a colony, and counts the number as a colony number.

  The colony detection determination unit 422 may detect the detected colony pixel (for example, the position on the medium image and the ID of the colony candidate to which the own pixel belongs), the detected colony candidate (for example, the arrangement position of the colony pixel on the medium image, color characteristics, or (Predetermined information such as the center of gravity position), the identified colony (for example, predetermined information such as the arrangement position of the colony pixel on the medium image, color characteristics, or the center of gravity position), and the counted number of colonies as the medium information via the registration processing unit 421 And stored in the medium information DB 401.

  In addition, the expansion process is one of image filter processes, and is a process of replacing all pixels adjacent to a colony pixel with colony pixels when other colony pixels exist adjacent to each colony pixel. .

  Further, the contraction process is a process of replacing all pixels adjacent to the colony pixel with non-colony pixels when there is a non-detected pixel adjacent to the colony pixel (hereinafter referred to as “non-colony pixel”). .

[5.3.3] Special Colony Detection Processing Next, details of the special colony detection processing in the colony detection determination unit 422 of the present embodiment will be described with reference to FIG. FIG. 7 is a diagram for explaining the special colony detection process of the colony detection determination unit 422.

  The colony detection determination unit 422 of the present embodiment performs a special colony detection process in which the work information acquired in the general colony detection process and the number of colonies detected by the general colony detection process are indicated by the condition information stored in the ROM / RAM 430. It is determined whether or not a determination for execution is provided.

  When the colony detection determination unit 422 determines that the determination condition is satisfied, the general-purpose colony detection process is a parameter used when detecting a colony, a detection criterion thereof, and a detection method of the colony. Two or more different special colony detection processes are executed.

For example, in the present embodiment, the condition information includes
(1) Escherichia coli (CC), Escherichia coli (EC), or Staphylococcus aureus (SA) as a bacterial species for executing the special colony detection process
(2) Processed food as the type of specimen (3) “0” as the threshold for the number of colonies
Is defined, the colony detection determination unit 422 executes the special colony detection process when any of the determination conditions of the acquired work information and the number of colonies detected by the general-purpose colony detection process is satisfied. That is, the colony detection determination unit 422 has the determination condition when the bacterial species to be examined is the coliform group, the sample is processed food, and the number of colonies detected by the general-purpose colony detection process is “0”. Then, based on the acquired culture medium information, a special colony detection process having higher colony detection sensitivity than the general colony detection process is executed.

  On the other hand, when the colony detection determination unit 422 executes the special colony detection process, the general colony detection process is to change the detection criteria and parameters, or change the detection method itself, that is, the same medium image, The colony detection process in the medium information is executed.

  Specifically, the colony detection determination unit 422 determines whether the colony candidate detected by the general colony detection process is a colony, that is, the colony detection sensitivity is higher than that of the general colony detection process. Change detection criteria to determine whether it is non-colony.

  For example, when executing the special colony detection process on the medium image as shown in FIG. 7, the colony detection determination unit 422 detects the colony in the RGB three-dimensional space in consideration of the color feature amount (blue) as the colony. A possible range (a range in a three-dimensional space defined by each gradation value of RGB) is determined in advance, colony candidates belonging to the range are detected as colonies, and are closer to the color of the medium (specifically, Detect colony candidates (thinner than normal colonies). That is, the colony detection determination unit 422 sets parameters and detection criteria so that the objects γ1 to γ4 that are not detected as colonies in the general-purpose colony detection process can be detected as colonies.

  In this embodiment, the colony detection determination unit 422 may use the same detection method as the general colony detection process, change the parameters and detection criteria in the detection method, and the general colony detection process. Differently, the detection method may be changed so that colonies closer to the color of the medium can be detected.

In this case, for example, when changing the parameters using the same detection method as the general-purpose colony detection process, the colony detection determination unit 422
(1) In order to increase the sensitivity for detecting a colony pixel, the threshold value of the color feature amount for detecting the colony pixel, such as a threshold value for binarization, is close to the color of the medium. Change it so that it can be detected as a colony pixel,
(2) Widen the range of gradation values of colony pixels that should be determined to be the same among colony pixels, facilitate the connection of colony pixels, and detect many colony candidates,
(3) Based on feature quantities such as color characteristics, roundness, shape, and size, the threshold value used when identifying a colony candidate as a colony based on the calculated likelihood is lower than the general-purpose colony detection process,
(4) Count the identified colonies.

  In addition, when the colony detection determination unit 422 changes the detection method, for example, the brightness is used as the color feature amount when the colony pixel is detected in the general-purpose colony detection process, while the color feature amount is used in the special colony detection process. Saturation is used as In addition, the colony detection determination unit 422 simply connects colony candidates based on the tone values of adjacent colony pixels in the general colony detection process, while using a brightness gradient between colony pixels in the special colony detection process. Connect with colony candidates.

[5.3.4] Medium determination processing Next, details of the medium determination processing in the colony detection determination unit 422 of the present embodiment will be described.

  The colony detection determination unit 422 of the present embodiment does not perform the special colony detection process when the number of colonies counted by the general colony detection process does not have a predetermined condition and performs the general colony detection process. Based on the number of colonies counted by the processing, the medium determination is executed.

  In addition, the colony detection determination unit 422 has a predetermined number of colonies counted by the general-purpose colony detection process, and when the special colony detection process is executed, the colony counted by the special colony detection process Based on the number, medium determination is performed.

  On the other hand, the colony detection determination unit 422 is obtained in the general colony detection process itself or the corresponding general colony detection process, whether the medium determination process is a general colony detection process or a special colony detection process. Based on the profile, the threshold value set in advance is compared with the number of colonies detected, and if the detected colony number is equal to or greater than the threshold value, it is determined as abnormal, and if it is smaller than the threshold value, it is normal And the medium determination of whether the specimen of each film type culture medium 60 is abnormal or normal (that is, pass / fail judgment of the specimen) is executed.

[5.4] Lot Determination Processing Unit Next, details of the lot determination processing unit 424 in the server device 40 of the present embodiment will be described.

  The lot determination processing unit 424 of the present embodiment analyzes data on hygiene management in the designated lot, work line 80 or process based on the instructions of the administrator input to the administrator terminal device 20 (ie, pass / fail determination). Execute.

  Specifically, the lot determination processing unit 424, for each lot, line information of the work line 80 used for the corresponding lot, and medium information registered in association with the corresponding work line 80 (at least the number of colonies). Based on the above, it is determined whether the food group manufactured or inspected under the same condition has a predetermined condition.

  In addition, when the lot determination is instructed, the lot determination processing unit 424 determines whether the work line 80 included in the corresponding lot and the processes belonging to the work line 80 are abnormal or normal in terms of hygiene management. 80 line determinations (that is, pass / fail determination for hygiene management) and process determinations for each process (that is, determination of pass / fail for hygiene management) are performed, and lot determination is performed using the process determination and line determination.

  In particular, when the lot determination is instructed, the lot determination processing unit 424, for example, the number of colonies of the film-type medium 60 in the medium information that has passed a predetermined culture inspection time such as 24 hours or 48 hours from the start of culture, Based on the medium information of the pass / fail determination of the specimen, the pass / fail of hygienic management of the process is determined, and the pass / fail of hygiene management of the work line 80 is determined based on the pass / fail determination of the process included in the corresponding work line 80. Based on the pass / fail determination of the work line 80 included in the corresponding lot, the pass / fail in sanitary management of the lot is determined.

  Note that the lot determination processing unit 424 of the present embodiment may execute not only lot determination but also process determination or line determination as data analysis.

(Process judgment)
The lot determination processing unit 424 has a culture start time at the same timing, a culture examination time at the same timing, a culture start time having a time within a preset time from the first time to the second time, or a preset first time. The culture start time or the culture test time has a predetermined condition, such as having a culture test time having a time within the time from the first time to the second time, and is registered in association with the same process in the same lot. Each process determination is performed based on the culture medium information of the plurality of film-type culture media 60 that has been performed. That is, the lot determination processing unit 424 executes each process determination by comprehensive determination based on each medium determination result of the plurality of film-type mediums 60.

  Specifically, the lot determination processing unit 424 displays the medium determination results of the corresponding film-type medium 60 (that is, all the medium determination results registered in the medium information having the process ID 71b of the process to be subjected to the process determination). In addition to obtaining, if the number of pass / fail of the judgment result satisfies a predetermined condition, the process judgment of the process to be judged is judged to be acceptable, and if not, the judgment should be made The process judgment of the process is judged as rejected.

  For example, the lot determination processing unit 424 determines that the conditions for all the film-type culture media 60 when the acceptable film-type culture media 60 are a predetermined ratio (60%) or more in the individual film-type culture media 60 in the corresponding process ( The number of colonies is equal to or less than a certain number), or the condition that the number of colonies of each film-type medium 60 corresponds (the average number of colonies of a single film-type medium 60 is equal to or less than a certain number). In this case, the corresponding process is determined to be acceptable.

  Note that the lot determination processing unit 424 of the present embodiment can change conditions depending on the type of specimen, the type of bacteria to be detected, etc., and can be set by an administrator or provided as part of a program. The

(Line judgment)
The lot determination processing unit 424 performs line determination for each work line 80 based on the pass / fail determination for hygiene management of a plurality of processes that are determined at a predetermined timing and registered in association with the corresponding line information. To do. That is, the lot determination processing unit 424 performs line determination by comprehensive determination based on the determination results of a plurality of processes of the same lot determined at a predetermined timing.

  Specifically, the lot determination processing unit 424 is determined at the same timing or a timing satisfying a predetermined condition, and has the line ID of the work line 80 to be subjected to line determination as described above. When the determination result of the determination is acquired and the number of pass / fail of the determination result satisfies a predetermined condition, the line determination of the work line 80 to be determined is determined to be acceptable, and the condition is not satisfied The line determination of the work line 80 to be determined is determined to be unacceptable.

  For example, when the three processes have the line ID of the work line 80 to be determined, the first process is “pass”, the second process is “pass”, and the third process is “fail”. In the case where the work line 80 is configured from a process in which the predetermined condition is “60%”, the lot determination processing unit 424 determines that the line determination of the work line 80 to be determined is acceptable.

(Lot judgment)
The lot determination processing unit 424 executes the lot determination for each lot based on the hygiene management pass / fail determination of the plurality of work lines 80 that are determined at a predetermined timing and registered in association with the corresponding lot information. To do. That is, the lot determination processing unit 424 executes lot determination by comprehensive determination based on the determination results of the plurality of work lines 80.

  Specifically, the lot determination processing unit 424 executes the lot determination based on the pass / fail determination in the work line 80 having the lot ID of the lot for which the lot determination is to be performed.

  For example, the lot determination processing unit 424 determines that the lot determination of the lot to be determined is acceptable when the line determination is successful in 60% of the work lines 80 having the lot ID, and the three work lines 80 determine the lot to be determined. Assume that a lot ID is included. In this case, when the first work line 80 is “pass”, the second work line 80 is “pass”, and the third work line 80 is “fail”, the lot determination processing unit 424 determines the lot to be determined. It is determined that the lot determination is acceptable.

  When the lot work time is long, the lot determination may be executed by dividing the time every predetermined time (for example, 8 hours or 12 hours). In this case, when the lot determination is performed while the work is suspended, a dummy result may be used, or it may be determined that the work is being suspended.

(Other)
The lot determination processing unit 424 can perform determinations and data analysis other than those described above using the culture medium information, line information, lot information, and work management DB 404, and the determination result or data analysis via the report processing unit 425. The result can be provided to the administrator terminal device 20 so that the administrator can view the result.

  Specifically, the lot determination processing unit 424 checks the reliability of the sample cultured in each film-type medium 60, that is, checks whether or not an inspection error has occurred. The medium information in the medium ID is subjected to a predetermined analysis of the medium information or the medium image data in time series from the culture start time to the end of the culture.

  For example, the lot determination processing unit 424 has a specific culture medium ID (same culture medium ID) 66 based on an instruction from the administrator received via the administrator terminal device 20, and starts from the culture start time to a predetermined time. Are extracted together with the medium image data. Then, the lot determination processing unit 424 performs a predetermined analysis on each medium information and each image data along a predetermined time series, or aggregates each medium information and each image data along a time series. In addition, the lot determination processing unit 424 causes the report processing unit 425 to generate the analysis result or the aggregation result as browsing data having a predetermined data format, and allows the administrator to browse the generated browsing data. 20 to provide.

  Note that the lot determination processing unit 424 is linked to the report processing unit 425, and at this time, based on an instruction from the manager terminal device 20, each medium information in one medium ID 66 is used for legal hygiene management reporting. May be made individually viewable.

  Further, the lot determination processing unit 424 may have a search function for medium information, line information, lot information, or work management information. Specifically, the lot determination processing unit 424 uses the lot ID (instruction ID 71a), the work line ID, the process ID 71b, the work ID 71, the culture start time, the lot work date and time, the culture medium ID 66, the specimen type, and the like as search keys. The database 400 may be searched, and the report processing unit 425 may be linked to generate data that allows the administrator to view the corresponding medium information and various types of information.

[5.5] Report Processing Unit Note that the report processing unit 425 determines the medium information (medium image data, the number of colonies, and the culture medium) specified by the search based on the administrator's instruction received via the administrator terminal device 20. (Judgment result), process information, line information, or lot-related information can be provided in a predetermined report format. That is, the report processing unit 425 is configured to be able to provide various information as evidence or reports in legal inspection reports and other cases.

[6] Hygiene management system operation process [6.1] Medium information registration process (portable communication terminal device)
Next, operation | movement of the culture medium information registration process in the portable communication terminal device 10 of this embodiment is demonstrated using FIG. FIG. 8 is a flowchart showing the culture information registration processing operation in the portable communication terminal device 10 of the present embodiment.

  In this operation, it is assumed that corresponding lot information and line information associated with the work instruction sheet 70 are already stored in the line information DB 402 and the lot information DB 403.

  Further, it is assumed that the medium ID 66 or the instruction ID 71a is attached to the film-type medium 60 and the work instruction sheet 70 by a two-dimensional barcode.

  Further, in this operation, the number of colonies in each film-type medium 60 is detected at the time of registration in the medium information database 400, and the portable communication terminal device 10 uses RGB for capturing a medium image. It is assumed that calibration for the gradation value or the luminance value is performed.

  First, in the portable communication terminal device 10, when the application control unit 120 detects an activation instruction for the culture medium registration application via the operation unit 170 (step S101), the application control unit 120 reads the culture medium registration application from the application storage unit 101 and activates it ( Step S102). At this time, the application control unit 120 performs initialization of the work memory and other necessary processes under the control of the mobile terminal management control unit 190.

  Next, the application control unit 120 displays an image for interlocking with the display control unit 161 to select whether the initial registration of the film-type medium 60 or the post-culture registration after a predetermined time has elapsed from the start of the culture. It is displayed on the unit 160 and waits for its input (step S103).

  Next, when detecting an input of a selection instruction from the operation unit 170 (step S104), the application control unit 120 determines whether initial registration or post-cultivation registration and sets the result in flag information (step S105).

  Next, the application control unit 120 causes the display unit 160 to display a screen that prompts the imaging of the film-type medium 60, and waits for imaging by the image data generation unit 110 in conjunction with the operation unit 170 (step S106). At this time, the application control unit 120 causes a predetermined image to be superimposed on the display unit 160 in order to align the medium image to be acquired.

  Next, when the application control unit 120 detects image capturing by the image data generation unit 110 in conjunction with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S107), the application data generation unit 110 and the medium ID 66 are detected. The imaged medium image data of the film-type medium 60 is acquired, and a predetermined image ID is assigned and stored in the image data storage unit 102 (step S108).

  Next, the application control unit 120 acquires the current time from the timer 180 as the imaging time of the medium image data, and stores it in the image data storage unit 102 as metadata in association with the medium image data (step S109).

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in a predetermined area of the acquired imaged culture medium image data, acquires the culture medium ID 66, and stores the image data in association with the culture medium image data. The information is stored in the unit 102 (step S110).

  Next, the application control unit 120 causes the display unit 160 to display a screen for acquiring the instruction sheet image data together with the instruction sheet ID 71a and the process ID 71b in conjunction with the display control unit 161, and waits for acquisition of the instruction sheet image data. (Step S111).

  Next, when the application control unit 120 detects imaging by the image data generation unit 110 in conjunction with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S112), the instruction sheet in which the work ID 71 is imaged. Image data is acquired and stored in the image data storage unit 102 in association with the culture medium image data acquired in step S108 (step S113).

  In addition, when acquiring the process ID 71b as the work ID 71, or when acquiring the one process ID 71b from the plurality of process IDs 71b, the application control unit 120 works in conjunction with the display control unit 161 and the operation unit 170 to inform the user. A display for selecting the process ID 71b to be acquired is displayed, and the corresponding process ID 71b is acquired.

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in the predetermined region of the captured instruction image data and the medium image data, acquires the work ID 71, and corresponds to the instruction image data In addition, the image data is stored in the image data storage unit 102 (step S114).

  Next, the application control unit 120 acquires the current time as the imaging time of the medium image data from the timer 180, and in conjunction with the display control unit 161 and the operation unit 170, the application control unit 120 acquires the acquired instruction image data, work ID 71, The medium image data, medium ID 66, and imaging time are confirmed (step S115).

  Note that the application control unit 120 may re-acquire the culture image data based on an instruction from the operator when the acquired medium information is confirmed by the operator. At this time, the application control unit 120 may be able to correct the medium image data by the operator in conjunction with the display control unit 161 and the operation unit 170.

  Next, based on the operator's instruction, the application control unit 120 uses the terminal ID and the work ID 71 as well as the acquired medium image data and its metadata, and flag information indicating whether initial registration or post-culture registration as medium information. Then, the data is transmitted to the server device 40 via the network communication unit 130 (step S116), and the registration process of the portable communication terminal device 10 is terminated.

  At this time, when transmitting the culture medium information, the network communication unit 130 executes login for accessing the server device 40 based on the terminal ID or the input ID and password, and the server device 40 is completed after the login is completed. Communication line is established and medium information is transmitted.

[6.2] Medium information registration processing (server device)
Next, the medium information registration processing operation in the server device 40 of the present embodiment will be described with reference to FIG. FIG. 9 is a flowchart showing the culture information registration processing operation in the server device 40 of this embodiment.

  In addition, this operation | movement is a process performed in conjunction with the culture medium information registration process performed by the portable communication terminal device 10, and the server apparatus 40 uses the culture medium information transmitted from the portable communication terminal apparatus 10 as a culture medium. This is a process for registering in the information DB 401.

  First, in the server device 40, when the communication control unit 410 receives the culture medium information transmitted from the portable communication terminal device 10 (step S300), the registration processing unit 421 is based on the flag information included in the received culture medium information. Whether the registration is initial registration or post-culture registration is determined (step S301).

  At this time, if the registration processing unit 421 determines initial registration, the process proceeds to step S302. If the registration processing unit 421 determines that it is not initial registration, that is, if it is determined to be post-culture registration, step S304 is performed. Move on to processing.

  Next, when it is determined as initial registration, the registration processing unit 421 extracts and specifies the work ID 71 included in the culture medium information (step S302).

  Next, the registration processing unit 421 registers the culture medium information in the culture medium information DB 401 based on the identified work ID 71 (step S303), and ends this operation. Although the culture start time is stored in advance in the work management DB 404, the registration processing unit 421 registers the imaging time included in the received medium information as the culture start time in the work management DB 404 in the process of step S303. Also good.

  On the other hand, if the registration processing unit 421 determines that the registration is after culture, it extracts and specifies the work ID 71 included in the culture medium information (step S304), and registers the culture medium information based on the imaging time included in the culture medium information. The culture examination time to be specified is specified (step S305).

  At this time, the registration processing unit 421 may be registered by designating a culture examination time for registering the culture medium information in conjunction with the portable communication terminal device 10, or the imaging time included in the received culture medium information. The culture examination time may be registered as it is. In addition, the registration processing unit 421 manages the culture medium registration at the culture test time that has already been performed, and specifies the culture test time based on the timing at which the culture medium information is received and the culture test time that has already been used to register the culture medium information. May be. That is, the registration processing unit 421 may specify the culture examination time by rewriting the execution flag each time the culture medium information is registered, referring to the information of the flag, and comparing the received imaging time.

Next, the registration processing unit 421 causes the colony detection determination unit 422 to execute a general-purpose colony detection process based on the acquired medium information and the specified work ID 71 (steps S307 to S309).
Specifically, the colony detection determination unit 422 specifies a profile for detecting a colony using the work information (step S307).

  And the colony detection determination part 422 performs image analysis with respect to the received culture medium image data based on the specified profile, and extracts the feature amount (luminance value) of each pixel constituting the culture medium image ( In step S308), colonies generated in the specimen imaged based on the feature amount of each pixel constituting the culture medium image are detected, and the number thereof is detected (step S309).

  On the other hand, the colony detection determination unit 422 acquires the condition information from the ROM / RAM 430, and the detection conditions (specifically, the specimen type and the bacterial species) included in the culture medium information provided from the registration processing unit 421 and step S309. It is determined whether or not the detected detection result (specifically, the number of colonies) satisfies the determination condition indicated by the acquired condition information (step S310). For example, in the present embodiment, the colony detection determination unit 422 is configured such that the specimen is processed food, the bacterial species is Escherichia coli, coliform group, or Staphylococcus aureus, and the number of colonies detected by the general-purpose colony detection process is In the case of “0”, it is determined that the determination condition is satisfied.

  At this time, if the colony detection determination unit 422 determines that the medium information provided from the registration processing unit 421 has the determination condition, the colony detection determination unit 422 executes a special colony detection process (step S311) and performs the process of step S312. If it is determined that the medium information provided from the registration processing unit 421 does not satisfy the determination condition, the process directly proceeds to step S312.

  In addition, the colony detection determination unit 422, as the special colony detection process, for the colony candidate detected by the general-purpose colony detection process, as described above, a predetermined color feature amount to be determined as a colony (for example, Based on RGB three-dimensional gradation values), re-detection is performed to identify colonies.

  Next, the colony detection determination unit 422 determines whether or not the detected number of colonies satisfies a predetermined condition based on the profile specified in the general-purpose colony detection process (step S312). That is, the colony detection determination unit 422 determines that it is acceptable (normal) when the predetermined condition is satisfied, and determines that it is unacceptable (abnormal) when the predetermined condition is not satisfied.

  The colony detection determination unit 422 is linked to the portable communication terminal device 10 to display the detected number of colonies and the result of the pass / fail determination on the display unit 160 of the portable communication terminal device 10 to allow the user to browse and check. You may do it.

  Next, the registration processing unit 421 specifies the culture start time information using the culture image data, the recognized medium ID 66, the determined pass / fail, the detected colony number, and the imaging time included in the medium information as the culture start time. The ID 71 is associated and registered in the culture medium information DB 401 (step S321), and this operation ends.

  The registration processing unit 421 displays line information and information to be registered on the display unit 160 of the portable communication terminal device 10, and based on a user instruction input via the portable communication terminal device 10, the culture medium Information or the like may be registered.

  Further, when the culture medium image data is transmitted from the portable communication terminal apparatus 10 and the medium ID 66 is recognized by analyzing each image data by the server apparatus 40, the registration processing unit 421 receives the reception in the process of step S201. The medium ID 66 imaged on each predetermined area of the medium image data thus obtained is analyzed, and the medium ID 66 is recognized and acquired. Similarly, the server device 40 also recognizes the work ID 71 in the instruction image data.

  Furthermore, although the above-mentioned operation is executed when the culture medium information is acquired by the portable communication terminal apparatus 10, the culture medium information acquired by the portable communication terminal apparatus 10 is recorded in the culture medium information DB 401 and then executed. May be.

[7] Modification [7.1] Modification 1
In this embodiment, instead of performing the culture information registration process using a portable communication terminal device 10 such as a tablet information terminal device, a smartphone, or a mobile phone, a laptop or desktop personal computer is used. The registration process of the culture medium information may be realized by a computer and an image input device such as a scanner, a digital camera, or a smartphone.

  In this case, the personal computer and the image input device may be connected according to a predetermined communication standard, and the registration process of the culture medium information may be realized by using the personal computer and the image input device integrally, or a memory card. The medium image data and instruction sheet image data previously acquired by the image input device are stored in other physical memory, and the registration process of the medium information is realized while the stored image data is captured by the personal computer. May be.

  In this case, the functions of the server device 40 may be provided in a personal computer to execute the various processes described above.

[7.2] Modification 2
In the present embodiment, the portable communication terminal device 10, the administrator terminal device 20, and the server device 40 may be installed or used in the same site, or each may be installed in a remote place such as outside the country or remotely. It may be used on the ground and each process described above may be executed. However, it is assumed that the portable communication terminal device 10 is used in the same lot.

[7.3] Modification 3
In the present embodiment, registration of the medium information in the specimen cultured on the film type medium 60 using the film type medium 60 is performed. However, the bacteria cultured on the agar medium using the agar medium and the bacteria Registration of medium information in a specimen may be executed.

[7.4] Modification 4
In the present embodiment, instead of acquiring the culture medium ID 66 by imaging the film-type culture medium 60 by the image data generation unit 110, the culture medium ID 66 may be input by an operator's operation input.

  In this case, the application control unit 120 interlocks with the display control unit 161 and the operation unit 170 and causes the operator to manually input the culture medium ID 66 directly using the operation unit 170.

  In addition, the application control unit 120 controls the display control unit 161 and the operation unit 170 to select a display item such as a pull-down menu by clicking or touch selection, and causes the operator to input the culture medium ID 66.

[7.5] Modification 5
In the present embodiment, various processes are executed by the single server device 40, but various processes may be executed by a server system including a plurality of server devices 40.

[7.6] Modification 6
In the present embodiment, the colony detection determination unit 422 executes the special colony detection process when the detection condition or the detection result in the general-purpose colony detection process such as the specimen type information, the bacterial species information, and the number of colonies includes the determination condition. However, the special colony detection process may be executed again when the conditions predetermined by the special colony detection process are not satisfied, such as when no colony is detected.

  In this case, the colony detection determination unit 422 may change the parameter, the inspection standard, or the detection method used for the special colony detection process, and execute the special colony detection process again. For example, the colony detection determination unit 422 changes the value that defines the detection criterion or the value of the parameter, and executes the second colony detection process again.

  Note that the colony detection determination unit 422 may repeatedly execute the special colony detection process until a condition predetermined by the special colony detection process such as until a colony is detected is satisfied. In this case, for example, the colony detection determination unit 422 repeatedly executes the second colony detection process while changing the value defining the detection criterion or the parameter value stepwise.

[7.7] Modification 7
In the present embodiment, the colony detection determination unit 422 manages the position on the medium image (the XY coordinate at the position of the center of gravity of the corresponding colony) when a colony is detected in the general colony detection process or the special colony detection process. The user terminal device 20 or the portable communication terminal device 10 performs image processing that can be displayed on the corresponding medium image, and the registration processing unit 421 registers the medium information including the image processing information in the medium information DB 401. May be.

[7.8] Modification 8
In the present embodiment, the colony detection determination unit 422 executes the special colony detection process when the detection condition or the detection result in the general-purpose colony detection process such as the specimen type information, the bacterial species information, and the number of colonies includes the determination condition. However, it may be performed based on an operator's instruction when imaging the film-type medium 60.

  In this case, the registration processing unit 421 determines whether the colony detection determination unit 422 has a determination condition when the received medium information includes an instruction from an operator forcibly executing the special colony detection processing. Regardless of whether or not, the special colony detection process is forcibly executed.

  As described above, in the sanitary management system S of the present embodiment, for example, when the colony is not detected when the general-purpose colony detection process is executed, it is determined that the predetermined condition is satisfied, and the colony detection sensitivity is set. To prevent overdetection and undetection of colonies, such as by setting parameters and detection criteria so as to be highly sensitive, or by performing special colony detection processing using a detection method, it is possible to prevent undetection. Can do.

  Therefore, in the sanitary management system S of the present embodiment, colony detection and counting can be accurately performed, and medium information including information regarding the accurately detected colonies and the number thereof is registered in the database. Can do.

  Further, in the sanitary management system S of the present embodiment, it is possible to save resources as compared with the case where various data analyzes and provisions are performed using physical resources such as paper media, and the culture medium. By registering the culture medium information in the database 400, registration mistakes can be remarkably reduced, and management of difficult culture media can be facilitated. Therefore, resource saving can be achieved without consuming wasteful culture media.

  In the sanitary management system S of the present embodiment, the description has been made using food. However, the present invention is not limited to the food, and can be applied to products that are provided to all living organisms including drugs and drugs. .

DESCRIPTION OF SYMBOLS S ... Hygiene management system 10 ... Portable communication terminal device 20 ... Manager terminal device 30 ... Network 40 ... Server device 60 ... Film type culture medium 70 ... Work instruction sheet 80 ... Work line 100 ... Data storage part 101 ... Application storage part 102 Image data storage unit 110 Image data generation unit 120 Application control unit 130 Network communication unit 140 Short-range wireless communication interface 150 Current position detection unit 160 Display unit 170 Operation unit 180 Timer 190 Mobile device management Control unit 400 ... database 401 ... medium information DB
402 ... Line information DB
403 ... Lot information DB
404 ... Work management DB
410 ... Communication control unit 420 ... Data processing unit 421 ... Registration processing unit 422 ... Colony detection determination unit 424 ... Lot determination processing unit 425 ... Report processing unit 430 ... Server management control unit

Claims (13)

An acquisition means for acquiring data of a medium image imaged by imaging a medium for culturing a colony as medium image data;
An extraction means for extracting a color feature amount relating to a color of a pixel constituting the culture medium image of the acquired culture medium image data;
Based on the color feature amount of the extracted pixel, identify a colony pixel constituting a colony in the medium image, detect the colony cultured in the medium based on the identified colony pixel, and First detection means for executing a first colony detection process for counting the detected colonies;
A determination unit that determines whether at least one of the detection condition and the detection result of the detection unit includes a predetermined determination condition;
When it is determined that the detection condition, the detection result, or both have predetermined determination conditions, a colony detection process different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means to be executed as a second colony detection process;
Registration means for registering information on colonies detected in one detection process of the first colony detection process or the second colony detection process in the database as medium information;
A culture medium information registration system comprising: In the culture medium information registration system according to claim 1,
When the second detection means determines that the detected number of colonies has a predetermined condition, based on the color feature amount of the extracted pixel, the first colony detection process determines the number of colonies. A culture medium information registration system that executes the second colony detection process with high detection sensitivity. In the culture medium information registration system according to claim 1 or 2,
When the second detection means determines that the detected number of colonies has a predetermined condition, the first colony detection process is based on the color feature amount of the extracted pixel. The culture medium information registration system which performs the said 2nd colony detection process from which the detection reference used when detecting this, the parameter at the time of detection, and the said colony detection method differ in 1 or 2 or more. In the culture medium information registration system according to any one of claims 1 to 3,
When the detection result of the second colony detection process does not have a predetermined condition, the second detection unit includes one or more of the detection standard, the parameter, and the colony detection method. The culture medium information registration system which changes and changes the said 2nd colony detection process again. In the culture medium information registration system according to claim 4,
The second detection means changes at least one of the detection standard, the parameter, and the colony detection method until the detection result of the second colony detection process satisfies a predetermined condition. A culture medium information registration system for repeatedly executing the second colony detection process. In the culture medium information registration system according to claim 4 or 5,
When the second detection means executes the second colony detection process again, medium information for changing the value defining the detection criterion or the parameter value and executing the second colony detection process again Registration system. In the culture medium information registration system according to any one of claims 1 to 6,
The culture medium information registration system, wherein the detection condition includes at least one of a type of the colony to be detected and a type of the specimen. In the culture medium information registration system according to any one of claims 1 to 7,
The culture information registration system, wherein the detection result includes at least the number of colonies detected. In the culture medium information registration system according to claim 8,
The medium information registration system, wherein the determination condition includes that the colony has not been detected by the first colony detection process. Computer
An acquisition means for acquiring data of a medium image imaged by imaging a medium for culturing a colony as medium image data,
Extraction means for extracting a color feature amount relating to a color of a pixel constituting the medium image of the acquired medium image data;
First, a colony pixel constituting a colony in the medium image is identified based on the color feature amount of the extracted pixel, the colony cultured in the medium is detected, and the detected colony is counted. First detection means for executing colony detection processing;
A determination means for determining whether at least one of the detection condition and the detection result of the detection means has a predetermined determination condition;
When it is determined that the detection condition, the detection result, or both have predetermined determination conditions, a colony detection process different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means to be executed as a second colony detection process; and
Registration means for registering information on colonies detected in at least one of the first colony detection process and the second colony detection process in the database as medium information;
Program to function as. An acquisition means for acquiring data of a medium image imaged by imaging a medium for culturing a colony as medium image data;
An extraction means for extracting a color feature amount relating to a color of a pixel constituting the culture medium image of the acquired culture medium image data;
First, a colony pixel constituting a colony in the medium image is identified based on the color feature amount of the extracted pixel, the colony cultured in the medium is detected, and the detected colony is counted. First detection means for executing a colony detection process;
A determination unit that determines whether at least one of the detection condition and the detection result of the detection unit includes a predetermined determination condition;
When it is determined that the detection condition, the detection result, or both have predetermined determination conditions, a colony detection process different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means to be executed as a second colony detection process;
A colony detection device comprising: Computer
An acquisition means for acquiring data of a medium image imaged by imaging a medium for culturing a colony as medium image data,
Extraction means for extracting a color feature amount relating to a color of a pixel constituting the medium image of the acquired medium image data;
Based on the color feature amount of the extracted pixel, identify a colony pixel constituting a colony in the medium image, detect the colony cultured in the medium based on the identified colony pixel, and A first detection means for executing a first colony detection process for counting the detected colonies;
A determination means for determining whether at least one of the detection condition and the detection result of the detection means has a predetermined determination condition; and
When it is determined that the detection condition, the detection result, or both have predetermined determination conditions, a colony detection process different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means to be executed as a second colony detection process;
A program characterized by functioning as A communication terminal device that generates a medium image imaged by imaging a medium for culturing colonies as medium image data;
A server device that detects colonies generated in the medium based on the medium image data and the number of the colonies, and registers the detection result in a database as medium information;
With
The server device is
Obtaining means for obtaining the medium image data;
An extraction means for extracting a color feature amount relating to a color of a pixel constituting the culture medium image of the acquired culture medium image data;
Based on the color feature amount of the extracted pixel, identify a colony pixel constituting a colony in the medium image, detect the colony cultured in the medium based on the identified colony pixel, and First detection means for executing a first colony detection process for counting the detected colonies;
A determination unit that determines whether at least one of the detection condition and the detection result of the detection unit includes a predetermined determination condition;
When it is determined that the detection condition, the detection result, or both have predetermined determination conditions, a colony detection process different from the first colony detection process is performed based on the color feature amount of the extracted pixel. Second detection means to be executed as a second colony detection process;
Registration means for registering information on colonies detected in the detection process of at least one of the first colony detection process and the second colony detection process in the database as medium information;
Hygiene management system characterized by comprising.