JP2015047136A5 - - Google Patents

Claims (6)

A cell culture vessel having a bottom surface and a side surface,
One or more microwells for containing cells are arranged on the bottom,
The first inclined surface formed so that the microwell becomes higher as it goes from the deepest part to the outer edge, and the second inclined surface that is connected to the first inclined surface and formed so as to become higher toward the outer edge and further toward the outer edge. An inclined surface,
An angle θ1 formed by a line perpendicular to the bottom surface of the cell culture container and the first inclined surface is less than 90 °,
The angle θ2 formed by the line perpendicular to the bottom surface of the cell culture container and the second inclined surface is 3 ° or more and 45 ° or less,
θ1 is larger than θ2,
The depth of the microwell is 0.05 mm or more and 0.5 mm or less,
Cell culture container. The cell culture container according to claim 1, wherein the opening width of the opening of the microwell is 0.1 mm or more and 1 mm or less. The cell culture container according to claim 1 or 2 , wherein an angle θ3 formed by the first inclined surface and the second inclined surface inside the microwell is 100 ° or more and 150 ° or less. The cell culture container according to any one of claims 1 to 3 , wherein a plurality of microwells are formed on a bottom surface, and an inner wall surrounding the plurality of microwells is formed. In the vertical cut surface of the microwell, curvature and the opening end the curvature of the connecting portion of the first inclined surface and the second inclined surface is 0.01mm or more 0.1mm or less, respectively, claim 1-4 1 The cell culture container according to Item. The cell according to any one of claims 1 to 5 , wherein the first inclined surface of the microwell forms a conical surface or a side surface of the truncated cone, and the second inclined surface forms a side surface of the truncated cone. Culture container.