JP2014228292A - Method for identifying pearl nucleus - Google Patents
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- JP2014228292A JP2014228292A JP2013105808A JP2013105808A JP2014228292A JP 2014228292 A JP2014228292 A JP 2014228292A JP 2013105808 A JP2013105808 A JP 2013105808A JP 2013105808 A JP2013105808 A JP 2013105808A JP 2014228292 A JP2014228292 A JP 2014228292A
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- bleached
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000004040 coloring Methods 0.000 claims abstract description 18
- 239000000975 dye Substances 0.000 claims abstract description 17
- 239000000985 reactive dye Substances 0.000 claims abstract description 13
- 108090000623 proteins and genes Proteins 0.000 abstract description 17
- 102000004169 proteins and genes Human genes 0.000 abstract description 17
- 239000011049 pearl Substances 0.000 description 93
- 238000004061 bleaching Methods 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 241000490567 Pinctada Species 0.000 description 6
- 239000007844 bleaching agent Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- XWGJFPHUCFXLBL-UHFFFAOYSA-M rongalite Chemical compound [Na+].OCS([O-])=O XWGJFPHUCFXLBL-UHFFFAOYSA-M 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 235000015170 shellfish Nutrition 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 241000237536 Mytilus edulis Species 0.000 description 2
- 238000003677 abuse test Methods 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000020638 mussel Nutrition 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 241000238366 Cephalopoda Species 0.000 description 1
- 241001481833 Coryphaena hippurus Species 0.000 description 1
- 241000510097 Megalonaias nervosa Species 0.000 description 1
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 239000000980 acid dye Substances 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 108010006161 conchiolin Proteins 0.000 description 1
- 239000010984 cultured pearl Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000000982 direct dye Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000984 vat dye Substances 0.000 description 1
Landscapes
- Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
本発明は、真珠核が漂白されているものか否かを判断するための、真珠核の識別方法に関する。 The present invention relates to a method for identifying a pearl nucleus for determining whether a pearl nucleus is bleached or not.
養殖真珠は、球状に加工した真珠核を、アコヤガイの体内に外套膜と共に挿入した後、そのアコヤガイを養殖して、真珠核表面に真珠層を形成させることで製造されている。ここで使用される真珠核は、一般に、淡水に生息するドブガイやヒレイケチョウガイ等の貝殻を球状に削って加工されたものが使用されている(例えば、特許文献1を参照)。 Cultured pearls are manufactured by inserting a spherically processed pearl nucleus into the body of an pearl oyster with a mantle and then culturing the pearl oyster to form a pearl layer on the surface of the pearl nucleus. As for the pearl nucleus used here, generally, a shell obtained by cutting a shell of a shell such as a dolphin or a flounder butterfly inhabiting in fresh water is used (see, for example, Patent Document 1).
ドブガイの貝殻が白色であるのに対し、ヒレイケチョウガイの貝殻は、オレンジ色や紫色を呈しており、この貝殻から真珠核を採取すると、白色系の真珠核と共に、何らかの色を帯びた非白色系の真珠核が生産される。ヒレイケチョウガイから白色系の真珠核を生産できる割合は20%程度に止まっており、薄い色がついている真珠核が30〜40%、残りの真珠核は濃い色を帯びている。 The white shell of the mussel is white, while the shell of the pearl oyster mussel is orange or purple. Pearl nuclei are produced. The ratio of white pearl nuclei that can be produced from the pearl oyster is only about 20%, 30-40% of pearl nuclei with a light color, and the remaining pearl nuclei are dark.
真珠核としては白色系のものが求められていることから、非白色系の真珠核を、ロンガリット等の漂白剤を用いてあらかじめ漂白し、白色系の真珠核に混入して販売される場合があった。 Since white pearl nuclei are required, non-white pearl nuclei may be pre-bleached with a bleaching agent such as Rongalite and mixed with white pearl nuclei for sale. there were.
しかしながら、真珠核が漂白されていると、真珠核の強度が低下してしまう問題がある。また、ロンガット漂白が施された真珠核は、水に浸漬すると有害なホルモリンを出すという問題もある。従って、漂白処理が施された真珠核は使用されるべきではない。 However, when the pearl nucleus is bleached, there is a problem that the strength of the pearl nucleus decreases. In addition, pearl nuclei subjected to longat bleach have a problem of producing harmful formolin when immersed in water. Therefore, pearl nuclei that have been bleached should not be used.
そのため、漂白された真珠核と、漂白されていない真珠核をあらかじめ見分ける方法が必要となる。そのような方法として、従来、ブラックライトを真珠核に照射する方法が行われている。この方法によると、漂白された真珠核には、青白い透明感のある明るさが認められる一方、漂白されていないものにはそのような明るさが認められないことから、目視によって両者を識別することが可能であった。 Therefore, a method for distinguishing a bleached pearl nucleus from an unbleached pearl nucleus in advance is required. As such a method, conventionally, a method of irradiating a pearl nucleus with black light has been performed. According to this method, bleached pearl nuclei have a pale and transparent brightness, while unbleached ones do not have such brightness. It was possible.
しかしながら、前述したブラックライトを用いた真珠核の識別方法では、検定結果にばらつきが生じる傾向があり、また、この方法のみでは漂白されている真珠核か否かを判断しにくい場合もあることから、真珠核の漂白の有無について、より確実で、簡易な識別方法の開発が望まれている。 However, in the method for identifying pearl nuclei using the black light described above, the test results tend to vary, and it may be difficult to determine whether the pearl nuclei are bleached by this method alone. Therefore, it is desired to develop a more reliable and simple identification method for the presence or absence of bleaching of pearl nuclei.
本発明は、上記現状に鑑み、貝殻から採取された真珠核が漂白されているものであるか否かを識別するための、より確実で、簡易な方法を提供することを目的とする。 The present invention has been made in view of the above situation, and an object of the present invention is to provide a more reliable and simple method for identifying whether or not a pearl nucleus collected from a shell is bleached.
貝殻から採取された真珠核に含まれる貝殻真珠層は、炭酸カルシウム層と、タンパク質層であるコンキオリンとの積層構造から構成されており、その特性として、色素が炭酸カルシウム層ではなく、タンパク質層に存在することが知られている。 The pearl layer contained in the pearl nucleus collected from the shell consists of a laminated structure of calcium carbonate layer and conchiolin, which is a protein layer. As a characteristic, the pigment is not in the calcium carbonate layer but in the protein layer. It is known to exist.
本発明者らが真珠核に対して虐待劣化試験及び強度テストを行って得られた結果から、漂白処理が施された真珠核では、色素を持っているタンパク質層が破壊されていると考えられた。 From the results obtained by the present inventors conducting abuse deterioration tests and strength tests on pearl nuclei, it is considered that the protein layer having the pigment is destroyed in the pearl nuclei subjected to the bleaching treatment. It was.
以上の知見に基づいて、本発明者らは、タンパク質と反応し得る染料を用いて真珠核を染色すれば、漂白処理が施されていない真珠核に含まれる破壊されていないタンパク質にのみ着色できる可能性があると推測し、その推測に基づき鋭意検討した結果、本発明を完成するに至った。 Based on the above findings, the present inventors can color only unbroken proteins contained in pearl nuclei that have not been bleached by staining pearl nuclei with dyes that can react with proteins. As a result of presuming that there is a possibility and intensively studying based on the presumption, the present invention has been completed.
すなわち本発明は、検査対象である真珠核をタンパク質反応性染料で処理した後、真珠核表面に付着した染料を除去し、その後、当該真珠核の着色状況を根拠にして、当該真珠核が漂白されているものか否かを判断することを特徴とする、真珠核の識別方法である。 That is, the present invention treats the pearl nucleus to be inspected with a protein-reactive dye, removes the dye attached to the surface of the pearl nucleus, and then bleaches the pearl nucleus based on the coloring state of the pearl nucleus. It is the identification method of the pearl nucleus characterized by determining whether it is what is done.
本発明を実施することで、貝殻から採取された真珠核において、漂白が施されることでタンパク質層が破壊されている場合には、当該真珠核の着色状況は、漂白されていない真珠核の着色状況と異なるので、真珠核の着色状況に基づいて、簡易かつ確実に、真珠核が漂白されているものか否かを識別することができる。 By carrying out the present invention, in the pearl nucleus collected from the shell, when the protein layer is destroyed by being bleached, the coloration state of the pearl nucleus is that of the unbleached pearl nucleus. Since it is different from the coloring situation, it is possible to easily and reliably identify whether or not the pearl nucleus is bleached based on the coloring situation of the pearl nucleus.
また、本発明によれば、漂白の有無だけではなく、生産から時間が経過して強度が低下している真珠核や、高温下での保存や日光照射により強度が低下している真珠核についても、識別することが可能である。 Further, according to the present invention, not only the presence or absence of bleaching, but also for pearl nuclei whose strength has been reduced over time from production, and for pearl nuclei whose strength has been reduced due to storage at high temperature or irradiation with sunlight. Can also be identified.
本発明では、真珠核と染料を接触させて一定時間加熱するという簡易な処理で漂白の有無の識別が可能になるので、実施が容易である。 In the present invention, since it becomes possible to identify the presence or absence of bleaching by a simple treatment of bringing the pearl nucleus and the dye into contact with each other and heating for a certain time, it is easy to implement.
以下に本発明の実施形態を説明する。 Embodiments of the present invention will be described below.
本発明の真珠核の識別方法では、まず、検査対象である真珠核をタンパク質反応性染料で処理する。この識別方法では、検査対象として、真珠核は1個のみを用いてもよいが、識別の精度を上げるために、複数の真珠核を用いることが好ましい。 In the pearl nucleus identifying method of the present invention, first, the pearl nucleus to be examined is treated with a protein reactive dye. In this identification method, only one pearl nucleus may be used as an inspection object, but it is preferable to use a plurality of pearl nuclei in order to increase the accuracy of identification.
本発明で用いるタンパク質反応染料とは、タンパク質と反応することで、当該タンパク質を含む材料を染めることが可能な染料であれば特に限定されない。タンパク質反応染料の具体例としては、直接染料、酸性染料、カチオン染料、建染染料、反応染料等が挙げられる。これらタンパク質反応染料を用いると、漂白処理が施されていない真珠核は着色されるが、漂白処理が施されてタンパク質が破壊又は変質している真珠核は、着色されない、あるいは、異なるように着色される。その結果、真珠核が漂白されているものか否かを識別することが可能となる。 The protein reactive dye used in the present invention is not particularly limited as long as it is a dye capable of dyeing a material containing the protein by reacting with the protein. Specific examples of protein reactive dyes include direct dyes, acid dyes, cationic dyes, vat dyes, reactive dyes and the like. When these protein reactive dyes are used, pearl nuclei that have not been bleached are colored, but pearl nuclei that have been bleached to destroy or alter proteins are not colored or colored differently. Is done. As a result, it is possible to identify whether or not the pearl nucleus is bleached.
タンパク質反応性染料としては1種類のみを使用してもよいし、複数を使用してもよい。複数使用する場合には、真珠核の着色に基づいた識別がより容易になるような、タンパク質反応性染料の組合せを用いることができる。 Only one kind or a plurality of protein reactive dyes may be used. In the case of multiple use, a combination of protein-reactive dyes can be used so that identification based on the coloration of the pearl nucleus becomes easier.
真珠核をタンパク質反応染料で処理する際の条件としては、使用するタンパク質反応染料の種類に応じて適宜決定することができる。一例としては、水の中に染料と真珠核を投入し、100℃以下の加温下で1分〜1時間程度保持すればよい。 The conditions for treating the pearl nucleus with a protein reactive dye can be appropriately determined according to the type of protein reactive dye used. As an example, a dye and a pearl nucleus may be put into water and kept at a temperature of 100 ° C. or lower for about 1 minute to 1 hour.
次に、染料で処理した系から真珠核を取り出した後、真珠核表面に付着した染料を除去する。除去する際の条件としては、染料が真珠核表面から十分に除去される条件を適宜決定すればよいが、一例として、適当な界面活性剤を投入した水中に真珠核を浸漬して、室温または100℃未満の加温下で1分〜1時間程度保持すればよい。これにより、余剰な染料を真珠核表面から取り除く。 Next, after removing the pearl nucleus from the system treated with the dye, the dye attached to the surface of the pearl nucleus is removed. The conditions for removal may be determined as appropriate so that the dye is sufficiently removed from the surface of the pearl nucleus, but as an example, the pearl nucleus is immersed in water charged with an appropriate surfactant, at room temperature or What is necessary is just to hold | maintain about 1 minute-1 hour under the heating of less than 100 degreeC. This removes excess dye from the pearl nucleus surface.
最後に、未反応の染料が除去された真珠核の着色の色及び程度に応じて、真珠核が漂白されているものか否かを判断する。着色の色及び程度に関しては、一例として、後述する実施例では、貝殻真珠層から採取され、かつ、漂白されていない真珠核はピンク色を呈する一方、漂白されている真珠核はピンク色以外の色に着色されるか、あるいは、着色されなかった。このような着色状況の結果に応じて、真珠核があらかじめ漂白されているものか否かを判断することができる。 Finally, it is determined whether or not the pearl nucleus is bleached depending on the color and degree of coloring of the pearl nucleus from which the unreacted dye has been removed. With regard to the color and degree of coloring, as an example, in the examples described below, unbleached pearl nuclei that are taken from the shell pearl layer exhibit a pink color, while bleached pearl nuclei are other than pink. Colored or not colored. Depending on the result of such a coloring situation, it can be determined whether or not the pearl nucleus has been previously bleached.
しかし、着色の色及び程度は、使用するタンパク質反応性染料の種類や量、あるいは、染料による処理条件等に異なるため、上記に限定されない。あらかじめ、漂白されていない真珠核及び漂白されている真珠核を準備し、所定の条件で、上述した染料の処理及び除去を実施して、各真珠核の着色状況を確認しておくことで、識別を実施する際の判断基準を作製しておけばよい。 However, the color and degree of coloring differ depending on the type and amount of the protein-reactive dye to be used, the treatment conditions with the dye, etc., and are not limited to the above. By preparing the unbleached pearl nuclei and the bleached pearl nuclei in advance, carrying out the treatment and removal of the above-described dye under predetermined conditions, and confirming the coloring status of each pearl nuclei, What is necessary is just to prepare the criteria for carrying out identification.
本発明の識別方法は、入手した多数の真珠核の中から、識別用のサンプルとして少数の真珠核を取り出してこれを検査に付することにより実施することができる。 The identification method of the present invention can be carried out by taking out a small number of pearl nuclei as a sample for identification from a large number of obtained pearl nuclei and subjecting them to inspection.
以下に実施例を掲げて本発明をさらに詳細に説明するが、本発明はこれら実施例に限定されるものではない。 The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.
(実施例1)
本実施例では、真珠核のサンプルとして、以下の表1に示すものを用いた。
Example 1
In this example, the pearl nucleus samples shown in Table 1 below were used.
各サンプルをポリエステル製の袋に投入し、複合染料で5分間煮沸した後、余剰な染料を除去し、各サンプルを乾燥させて着色状況を目視にて評価した。 Each sample was put into a polyester bag and boiled with a composite dye for 5 minutes, after which excess dye was removed, each sample was dried, and the coloring state was visually evaluated.
その結果、サンプル1−10はピンク色に着色されているが、漂白処理が施されているサンプル11−15は、着色されないか、あるいは、ピンク色とは明らかに異なる紫がかった色や緑色に着色された。このことから、ピンク色に着色された真珠核は漂白処理が施されていない真珠核であり、着色されないか、又はピンク色以外の色に着色された真珠核は漂白処理があらかじめ施されている真珠核であると判断することができる。 As a result, sample 1-10 is colored pink, but sample 11-15, which has been bleached, is not colored or has a purplish color or green that is clearly different from pink. Colored. From this, pearl nuclei colored in pink are pearl nuclei that have not been bleached, and pearl nuclei that are not colored or colored in a color other than pink are pre-bleached It can be judged that it is a pearl nucleus.
サンプル1〜3は、いずれもピンク色に着色されたが、サンプル2のピンク色が最も鮮やかであったことから、漂白処理が施されていない真珠核の中でも、新しい真珠核は着色度合が強く、製造年数が経過するにつれ、着色度合が薄くなることが分かった。 Samples 1 to 3 were all colored pink, but the pink color of Sample 2 was the most vivid, so the new pearl nuclei have a strong degree of coloring among the pearl nuclei that have not been bleached. It has been found that the coloring degree becomes lighter as the manufacturing years elapse.
サンプル7〜9の結果から、原貝の種類によって着色度合に若干の相違があったが、漂白処理が施されていないサンプルは、原貝の種類に関わらず、いずれもピンク色に着色された。このことから、本発明は原貝の種類によらず適用できることが分かった。 From the results of Samples 7 to 9, there was a slight difference in the degree of coloring depending on the type of raw shellfish, but the samples that were not bleached were all colored pink regardless of the type of raw shellfish. . From this, it was found that the present invention can be applied regardless of the type of raw shellfish.
また、サンプル10の再生核は、ピンク色に着色されたので漂白処理が施されていないと判断される。しかし、着色度合のばらつきが大きかった。これは、種々の履歴の真珠核が混在していることによるものと考えられる。 Moreover, since the reproduction nucleus of the sample 10 is colored pink, it is determined that the bleaching process has not been performed. However, the variation in the degree of coloring was large. This is thought to be due to the presence of various pearl nuclei.
サンプル11は、無漂白のサンプル1に対して、漂白剤としてロンガリットを使用して22時間の漂白を行って得たサンプルである。着色試験の結果、サンプル1がピンク色に着色したのに対し、サンプル11は緑色に着色した。 Sample 11 is a sample obtained by subjecting unbleached sample 1 to bleaching for 22 hours using Rongalite as a bleaching agent. As a result of the coloring test, sample 1 was colored green while sample 11 was colored green.
サンプル12〜14は、無漂白のサンプル2に対して、漂白剤としてロンガリットを使用してそれぞれ8時間、14時間、22時間と漂白時間を変更して漂白を行って得たサンプルである。着色試験の結果、サンプル2がピンク色に着色したのに対し、22時間の漂白を施したサンプル14は、まったく着色しなかった。一方、8時間又は14時間の漂白を施したサンプル12及び13は、紫色に着色した。 Samples 12 to 14 are samples obtained by bleaching unbleached sample 2 using Rongalite as a bleaching agent and changing the bleaching time to 8 hours, 14 hours, and 22 hours, respectively. As a result of the coloring test, sample 2 was colored pink, whereas sample 14 subjected to bleaching for 22 hours was not colored at all. On the other hand, Samples 12 and 13 that had been bleached for 8 hours or 14 hours were colored purple.
サンプル15は、漂白はされているものの、漂白時間が不明なサンプルであるが、サンプル14と同様、着色しなかった。 Sample 15 was bleached but the bleaching time was unknown, but, like sample 14, it was not colored.
(実施例2)
本実施例では、以下の4種類の真珠核を用いた。
1.中国製イケチョウ核
2.日本製米国産三山核
3.日本製米国産ニガーヘッド核
4.日本製米国産ウォッシュボード核
これらの真珠核に対して下記の虐待試験のいずれかを行った。
1.水漂白。35%過酸化水素水を水で3%に希釈した溶液に7日間浸漬。
2.メタノール漂白。35%過酸化水素水をメタノールで3%に希釈した溶液に7日間浸漬。
3.加温メタノール処理。50℃に加温したメタノールに1晩浸漬。
4.日光照射(7日)
5.80℃で加熱(7日)
6.無処理(対照区)
虐待試験後、4種類の核について6試験ずつ、全部で24試料について染色試験を実施した。
(Example 2)
In this example, the following four kinds of pearl nuclei were used.
1. Chinese squid butterfly nucleus 2. US made Sanzan core 3. 3. Japanese niger head core made in Japan US-made washboard nuclei made in Japan One of the following abuse tests was performed on these pearl nuclei.
1. Water bleaching. Immerse in a solution of 35% hydrogen peroxide solution diluted to 3% with water for 7 days.
2. Methanol bleaching. Immerse in a solution of 35% hydrogen peroxide solution diluted to 3% with methanol for 7 days.
3. Warm methanol treatment. Soak overnight in methanol heated to 50 ° C.
4). Sunlight irradiation (7th)
5. Heat at 80 ° C (7 days)
6). No treatment (control zone)
After the abuse test, staining tests were performed on a total of 24 samples, 6 tests for each of the four nuclei.
その結果、無処理の真珠核がピンク色に着色したのに対し、W漂白を施した真珠核は、濃い紫色に着色した。HotMeOH前処理を施した真珠核は、無処理の真珠核と同程度の着色度合であった。一方、M漂白、日光照射、及び加熱による処理を施した真珠核は、無処理の真珠核よりも薄い色目に着色された。 As a result, untreated pearl nuclei were colored pink, whereas pearl nuclei subjected to W bleaching were colored deep purple. The pearl nuclei subjected to the hot MeOH pretreatment had the same degree of coloring as the untreated pearl nuclei. On the other hand, the pearl nuclei treated with M bleaching, sunlight irradiation, and heating were colored to a lighter color than the untreated pearl nuclei.
実施例1との対比から、本発明の識別方法を使用すると、ロンガリット等の還元漂白剤による処理を施された真珠核を最も有効に識別することができるが、酸化水素等の酸化漂白剤による処理を施された真珠核や、高温下での保存や日光照射に晒された真珠核をも識別できる可能性があることが示された。 From the comparison with Example 1, when the identification method of the present invention is used, pearl nuclei treated with a reducing bleach such as Rongalite can be most effectively identified. It has been shown that pearl nuclei that have been treated and pearl nuclei that have been stored at high temperatures or exposed to sunlight may be identified.
本発明によれば、真珠核をアコヤガイの体内に挿入する前の段階で、真珠核に漂白処理が施されているものか否かを識別することができるため、アコヤガイの養殖により真珠を製造するにあたって、漂白処理により強度が低下した真珠核の使用を回避することができる。
According to the present invention, since it is possible to identify whether or not the pearl nucleus has been subjected to bleaching treatment before the pearl nucleus is inserted into the body of the pearl oyster, the pearl is produced by culturing the pearl oyster. In doing so, it is possible to avoid the use of pearl nuclei whose strength has been reduced by bleaching.
Claims (1)
After the pearl nucleus to be inspected is treated with a protein-reactive dye, the dye attached to the pearl nucleus surface is removed, and then the pearl nucleus is bleached based on the coloring situation of the pearl nucleus A method for identifying a pearl nucleus, characterized by determining whether or not.
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