JP2014150761A5

JP2014150761A5 -

Info

Publication number: JP2014150761A5
Application number: JP2013023123A
Authority: JP
Filing date: 2013-02-08
Publication date: 2016-03-17
Anticipated expiration: 2033-02-08

Claims

An anti-human NGF antibody Fab ′ fragment comprising:
Heavy chain variable comprising CDR1 consisting of amino acid sequence 31-35 of SEQ ID NO: 6, CDR2 consisting of amino acid sequence 50-65 of SEQ ID NO: 6, and CDR3 consisting of amino acid sequence 98-110 of SEQ ID NO: 6 Area; and
Light chain variable comprising CDR1 consisting of the 24th to 39th amino acid sequence of SEQ ID NO: 4, CDR2 consisting of the 55th to 61st amino acid sequence of SEQ ID NO: 4, and CDR3 consisting of the 94th to 102nd amino acid sequence of SEQ ID NO: 4 region
An anti-human NGF antibody Fab ′ fragment.

Comprising a heavy chain constant region is a human Igγ1 constant region, Fab 'fragment according to claim 1.

It comprises a light chain constant region is a human Igκ constant region, Fab 'fragment according to claim 1.

It comprises a light chain constant region is a heavy chain constant region and the human Igκ constant region Ru human Igγ1 constant region der, Fab 'fragment according to claim 1.

The Fab ′ fragment according to any one of claims 1 to 4 , wherein polyethylene glycol is bound thereto.

An anti-human NGF antibody Fab ′ fragment comprising culturing a host cell selected from the group consisting of the following (a) and (b) to express an anti-human NGF antibody Fab ′ fragment: Anti-human NGF antibody Fab ′ fragment produced by the method of producing antibody Fab ′ fragment:
(A) a polynucleotide comprising a heavy chain fragment comprising a heavy chain variable region comprising the amino acid sequence represented by SEQ ID NO: 6 and a sequence encoding a light chain comprising the light chain variable region comprising the amino acid sequence represented by SEQ ID NO: 4 A host cell transformed with an expression vector comprising; and
(B) an expression vector comprising a polynucleotide comprising a sequence encoding a heavy chain fragment comprising a heavy chain variable region comprising the amino acid sequence represented by SEQ ID NO: 6, and a light chain variable region comprising the amino acid sequence represented by SEQ ID NO: 4 A host cell transformed with an expression vector comprising a polynucleotide comprising a sequence encoding a light chain comprising.

An anti-human NGF antibody Fab ′ fragment comprising culturing a host cell selected from the group consisting of the following (a) and (b) to express an anti-human NGF antibody Fab ′ fragment: Anti-human NGF antibody Fab ′ fragment produced by the method of producing antibody Fab ′ fragment:
(A) an expression vector comprising a polynucleotide comprising a heavy chain fragment consisting of the amino acid sequence shown in SEQ ID NO: 10, SEQ ID NO: 14 or SEQ ID NO: 16 and a sequence encoding a light chain consisting of the amino acid sequence shown in SEQ ID NO: 12 A host cell transformed with; and
(B) an expression vector comprising a polynucleotide comprising a heavy chain fragment comprising the amino acid sequence represented by SEQ ID NO: 10, SEQ ID NO: 14 or SEQ ID NO: 16 and a light chain comprising the amino acid sequence represented by SEQ ID NO: 12 A host cell transformed with an expression vector comprising a polynucleotide comprising a sequence encoding

The Fab 'fragment according to claim 6 or 7, conjugated with polyethylene glycol.

An anti-human NGF antibody Fab fragment comprising:
Heavy chain variable comprising CDR1 consisting of the 31st to 35th amino acid sequences of SEQ ID NO: 2, CDR2 consisting of the 50th to 65th amino acid sequences of SEQ ID NO: 2, and CDR3 consisting of the 98th to 110th amino acid sequences of SEQ ID NO: 2 Area; and
Light chain variable comprising CDR1 consisting of the 24th to 39th amino acid sequence of SEQ ID NO: 4, CDR2 consisting of the 55th to 61st amino acid sequence of SEQ ID NO: 4, and CDR3 consisting of the 94th to 102nd amino acid sequence of SEQ ID NO: 4 region
An anti-human NGF antibody Fab fragment.

An anti-human NGF antibody Fab fragment comprising:
Heavy chain variable comprising CDR1 consisting of amino acid sequence 31-35 of SEQ ID NO: 6, CDR2 consisting of amino acid sequence 50-65 of SEQ ID NO: 6, and CDR3 consisting of amino acid sequence 98-110 of SEQ ID NO: 6 Area; and
Light chain variable comprising CDR1 consisting of the 24th to 39th amino acid sequence of SEQ ID NO: 4, CDR2 consisting of the 55th to 61st amino acid sequence of SEQ ID NO: 4, and CDR3 consisting of the 94th to 102nd amino acid sequence of SEQ ID NO: 4 region
An anti-human NGF antibody Fab fragment.

An anti-human NGF antibody Fab fragment comprising a heavy chain variable region consisting of the amino acid sequence shown in SEQ ID NO: 2 or 6 and a light chain variable region consisting of the amino acid sequence shown in SEQ ID NO: 4.