JP2014092422A - Specimen inspection chip - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a specimen inspection chip for stably detecting biological information from a specimen disposed in an inspection device with the specimen injected therein.SOLUTION: The specimen inspection chip includes: an injection port for injecting a specimen; a holding chamber for holding the specimen injected from the injection port; plural flow paths 4 for allowing the specimen flowing being separated from the holding chamber; plural measuring chambers 5 for allowing the specimen flowing in via plural flow paths 4 to reacts with a reagent held therein; and plural sealed parts 6 filled with thermal melting agent disposed in the flow path 4. Each of the sealed parts 6 has a reservoir 7 in a direction crossing the flow path.

Description

  The present invention relates to a sample test chip, and more particularly to a sample test chip that is arranged in a test apparatus in a state where a sample such as blood is injected and detects biological information from the sample.

  Conventionally, as a specimen testing chip used as described above, an inlet for injecting a specimen, a holding chamber for holding a specimen injected from the inlet, and a specimen flowing from the holding chamber branch and flow. Filled in a plurality of flow paths, a plurality of measurement chambers for reacting each of the specimens flowing in through the plurality of flow paths, and a reagent held therein, and a closing portion provided in the plurality of flow paths A temperature melting agent (for example, Patent Document 1).

International Publication No. 2008/106719

  The problem in the above-described conventional example is that the test becomes unstable when the sample test chip is used.

  That is, such a specimen testing chip is arranged in a testing apparatus, and rotates and drives the specimen injected into the holding chamber to supply the measurement chamber through a plurality of flow paths using centrifugal force. The sample is reacted with the reagent in this measurement chamber, and thereby the specimen is inspected.

  At the time of such an inspection, the temperature melting agent provided at the closing portion of the flow path is melted by heating, and as a result, as described above, the specimen injected into the holding chamber has a plurality of flows due to centrifugal force. The sample is supplied to the measurement chamber via the channel and reacted with the reagent in the measurement chamber, so that the specimen can be inspected.

  In such a specimen test chip, the temperature melting agent such as wax applied to the flow path is required to start to melt quickly when the specimen test chip reaches a predetermined temperature. The amount is very small.

  However, when the flow path closing portion is closed by such a small amount of temperature melting agent, when this specimen test chip is rotationally driven, it receives the centrifugal force and moves to the measurement chamber side. As a result, the specimen flows into only some of the measurement chambers, which makes the examination unstable.

  Accordingly, an object of the present invention is to stabilize a test using a sample test chip.

In order to solve the above-described problems, a sample test chip according to the present invention includes an injection port for injecting a sample, a holding chamber for holding a sample injected from the injection port, and a sample flowing from the holding chamber. A plurality of measurement chambers for reacting a plurality of flow channels flowing through the plurality of flow channels, a sample flowing in through each of the plurality of flow channels, and a reagent held therein, and a closing portion provided in the plurality of flow channels And a reservoir portion is provided in the closing portion in a direction crossing the flow path, thereby achieving an intended purpose.

  As described above, the sample test chip according to the present invention includes an injection port for injecting a sample, a holding chamber for holding a sample injected from the injection port, and a plurality of samples flowing from the holding chamber flowing in a branched manner. A flow path, a plurality of measurement chambers for reacting each of the specimens flowing in through the plurality of flow paths, and a reagent held therein, and a temperature filled in a closing portion provided in the plurality of flow paths And a reservoir is provided in the closing portion in a direction crossing the flow path, so that a test using this specimen test chip can be stabilized.

  In other words, in the present invention, since the closing portion is provided with a reservoir portion in a direction crossing the flow path, the temperature melting agent crosses the flow path even if a centrifugal force is applied to the sample test chip. As a result, the temperature melting agent does not inadvertently flow into the measurement chamber, and the inflow timing of the sample flowing into each measurement chamber is almost the same. In conclusion, the test using this sample test chip can be stabilized.

Configuration diagram of the specimen testing chip in the first embodiment of the present invention (A) is an enlarged view of the vicinity of the closing portion of the sample test chip in Embodiment 1 of the present invention, and (b), (c), and (d) are enlarged views showing other embodiments of the present invention, respectively. Overview of specimen test chip and biochemical analyzer according to Embodiment 1 of the present invention

Hereinafter, a sample test chip according to an embodiment of the present invention will be described in detail with reference to the drawings.
(Embodiment 1)
As shown in FIG. 1, the sample test chip 1 of the present embodiment is supplied from an injection port 2 for injecting a sample, a holding chamber 3 for holding a sample injected from the injection port 2, and a holding chamber 3. A plurality of flow channels 4 in which the sample to be branched flows, a plurality of measurement chambers 5 for reacting the samples respectively flowing through the plurality of flow channels 4 and the reagent held therein,
And a temperature melting agent (for example, wax) filled in the closing portions 6 provided in the plurality of flow paths 4. Further, as shown in FIG. 2A, the closing portion 6 is provided with a reservoir portion 7 in a direction intersecting with the flow path 4.

  The reservoir 7 has a rectangular shape (a cylinder as an actual three-dimensional shape) as shown in the plan view of FIG. 2 (a), but has an elliptical shape as shown in the plan view of FIG. 2 (b). Even if it has a shape with rounded corners relative to the rectangle as shown in FIG. 2C, it may have a hexagonal shape as shown in the plan view of FIG. In any shape, the reservoir 7 is formed in a direction crossing the flow path 4 other than on the extension of the flow path 4, and the filled temperature melting agent is caught in the reservoir 7. is there.

As described above, in the present embodiment, the reservoir 6 is provided in the direction intersecting the flow path 4 in the closing portion 6, so that even if centrifugal force is applied to the sample test chip 1, the temperature melting agent Is in a so-called hooked state in the reservoir 7 provided in the direction crossing the flow path 4. As a result, the temperature melting agent does not inadvertently flow into the measurement chamber 5, whereby the inflow timing of the sample flowing into each measurement chamber 5 becomes substantially constant. As a result, the test using this sample test chip 1 is performed. Can be stabilized.

  Further, as shown in FIG. 2A, a branch portion 8 is provided at the axial center portion of the flow path in the closing portion 6. The branch portion 8 is formed by a protrusion that protrudes from the inner surface of the closing portion 6 toward the axial center of the flow path.

  As described above, in the present embodiment, by providing the branch portion 8 at the axial center portion of the flow path 4 in the closing portion 6, when the temperature melting agent is melted by heating and the specimen flows into the measurement chamber 5, Since it works to prevent the flow of the specimen, the possibility of the temperature melting agent inadvertently flowing into the measurement chamber 5 can be further lowered, and the examination using the specimen test chip 1 can be further stabilized. .

  Next, the biochemical analyzer 9 using the sample test chip 1 in the present embodiment will be described with reference to FIG. The biochemical analyzer 2 is a device for detecting and analyzing a living body in a hospital, a clinic, or the like. Specifically, the specimen test chip 1 on which the specimen is spotted is set from the insertion port 10 provided in the biochemical analyzer 9, and the detection target is detected. A touch panel 11 capable of displaying the measurement result is provided on the front surface of the biochemical analyzer 9. The user performs operations such as measurement start and stop using the touch panel 11.

  As described above, since the sample test chip according to the present invention can stabilize the test, the sample test chip is arranged in the test apparatus in a state in which a sample such as blood is injected. This is useful in a biochemical analyzer that detects information.

DESCRIPTION OF SYMBOLS 1 Specimen inspection chip 2 Inlet 3 Holding chamber 4 Flow path 5 Measurement chamber 6 Closure part 7 Reservoir part 8 Branch part 9 Biochemical analyzer 10 Input port 11 Touch panel

Claims (3)

An inlet for injecting a specimen;
A holding chamber for holding a specimen injected from the inlet;
A plurality of flow paths through which the analyte flowing in from the holding chamber flows;
A plurality of measurement chambers for reacting each of the specimens flowing in through the plurality of flow paths and the reagent held therein;
A temperature melting agent filled in a closing portion provided in the plurality of flow paths,
The specimen testing chip according to claim 1, wherein a reservoir is provided in the closing portion in a direction intersecting the flow path. The specimen test chip according to claim 1, wherein a branch portion is provided in an axial center portion of the flow path in the closing portion. The specimen test chip according to claim 2, wherein the branch portion is formed by a protrusion protruding from the inner surface of the closing portion toward the axial center of the flow path.