JP2014061459A5 - - Google Patents
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- Publication number
- JP2014061459A5 JP2014061459A5 JP2012206518A JP2012206518A JP2014061459A5 JP 2014061459 A5 JP2014061459 A5 JP 2014061459A5 JP 2012206518 A JP2012206518 A JP 2012206518A JP 2012206518 A JP2012206518 A JP 2012206518A JP 2014061459 A5 JP2014061459 A5 JP 2014061459A5
- Authority
- JP
- Japan
- Prior art keywords
- extracting
- metabolite
- organic solvent
- solution
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000003960 organic solvent Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000002207 metabolite Substances 0.000 claims 11
- WEVYAHXRMPXWCK-UHFFFAOYSA-N acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 2
- 238000000605 extraction Methods 0.000 claims 2
- 239000007791 liquid phase Substances 0.000 claims 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 230000001464 adherent Effects 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 230000001629 suppression Effects 0.000 claims 1
- -1 thiol compound Chemical class 0.000 claims 1
- 239000012498 ultrapure water Substances 0.000 claims 1
Description
又、前記第１ステップで添加する有機溶媒を、水を含む第１の溶液とすることができる。 Further, the organic solvent added in the first step can be a first solution containing water .
又、前記第２ステップで添加する水を、有機溶媒を含む第２の溶液とすることができる。 Further, the water added in the second step may be a second solution containing organic solvent.
又、前記第１の溶液と前記第２の溶液の合計量に対する前記第１の溶液中の有機溶媒と前記第２の溶液中の有機溶媒の合計量の割合を５０％以上とすることができる。 The ratio of the total amount of the organic solvent in the first solution and the organic solvent in the second solution to the total amount of the first solution and the second solution can be 50% or more. .
Claims (9)
まず、水と任意に混合する有機溶媒を添加して、酸化を抑制しながら酵素を失活させる第１ステップと、
次いで、水を添加することにより、親水性の代謝物を液相に溶解させる第２ステップと、
その後、液相を分取する第３ステップとを含むことを特徴とする代謝物の抽出方法。 When extracting metabolites,
First, a first step of adding an organic solvent arbitrarily mixed with water to deactivate the enzyme while suppressing oxidation,
Next, a second step of dissolving the hydrophilic metabolite in the liquid phase by adding water;
And a third step of separating the liquid phase.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012206518A JP6173667B2 (en) | 2012-09-20 | 2012-09-20 | Metabolite extraction method |
| PCT/JP2013/074319 WO2014045932A1 (en) | 2012-09-20 | 2013-09-10 | Method for extracting metabolites |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012206518A JP6173667B2 (en) | 2012-09-20 | 2012-09-20 | Metabolite extraction method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014061459A JP2014061459A (en) | 2014-04-10 |
| JP2014061459A5 true JP2014061459A5 (en) | 2015-11-05 |
| JP6173667B2 JP6173667B2 (en) | 2017-08-02 |
Family
ID=50341246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012206518A Active JP6173667B2 (en) | 2012-09-20 | 2012-09-20 | Metabolite extraction method |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP6173667B2 (en) |
| WO (1) | WO2014045932A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614212B (en) * | 2015-01-20 | 2017-06-06 | 济宁神康生物科技有限公司 | A kind of method that sample pretreatment is analysed in attached cell Quantitative metabolite group credit |
| US10927401B2 (en) | 2015-09-11 | 2021-02-23 | Keio University | Method for extracting substance from feces sample |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4840804B2 (en) * | 2006-01-20 | 2011-12-21 | 学校法人慶應義塾 | Determination method of oxidative stress |
| EP2273267A1 (en) * | 2009-07-08 | 2011-01-12 | BASF Plant Science GmbH | Methods or analyzing polar metabolites of the engergy metabolism |
| KR101867808B1 (en) * | 2010-06-18 | 2018-06-18 | 각고호우징 게이오기주크 | Liver disease marker, method and apparatus for measuring same, and test method for pharmaceutical preparation |
-
2012
- 2012-09-20 JP JP2012206518A patent/JP6173667B2/en active Active
-
2013
- 2013-09-10 WO PCT/JP2013/074319 patent/WO2014045932A1/en active Application Filing
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