JP2014028787A5 - - Google Patents

Claims (14)

Keratinocytes (keratinocytes, keratinocyte) selects a whitening agent and an indicator cell adhesion inhibitory effect, the screening method of whitening. The step of evaluating the cell adhesion inhibitory action of keratinocytes seen including,
The process, keratinocytes were added a test substance, and in keratinocytes without addition of the test substance, integrin production inhibitory effect of keratinocytes, adhesion inhibitory action to the basal membrane of keratinocytes, and keratinocytes It is performed by comparing one or more kinds of actions selected from the group consisting of the upper layer movement promoting action,
The whitening agent screening method according to claim 1 , wherein the test substance is selected as a whitening agent when the action on the keratinocytes to which the test substance is added is greater than that of the keratinocytes to which the test substance is not added . The method for screening a whitening agent according to claim 2 , comprising a step of imparting a cell adhesion promoting factor to keratinocytes .   The whitening agent screening method according to claim 3, wherein the cell adhesion promoting factor is melanin and / or a substance related thereto. The whitening agent screening method according to any one of claims 2 to 4, wherein the integrin production inhibitory action of keratinocytes is an integrin alpha 6 and / or beta 4 gene expression inhibitory action.   The whitening agent screening method according to claim 5, wherein the integrin α6 and / or β4 gene expression inhibitory effect is an integrin α6 and / or β4 mRNA expression inhibitory effect. The method for screening a whitening agent according to any one of claims 2 to 4, wherein the integrin production inhibitory effect of keratinocytes is an integrin α6 and / or β4 protein expression inhibitory effect. The whitening agent screening according to any one of claims 2 to 4, wherein the inhibitory action of keratinocytes on the basement membrane is an inhibitory action of keratinocytes on Laminin-5. Method. Step performs subscription over training method whitening agent according to any one of claims 1 to 8, and also contain a whitening agent selected by the process, the design method of whitening composition. The design method according to claim 9, wherein the whitening composition is an external preparation for skin. The design method according to claim 9 or 10, wherein the whitening composition is a cosmetic (including a quasi-drug).   A whitening agent comprising a cell adhesion inhibitor for keratinocytes.   The cell adhesion inhibitor of keratinocytes is one or more kinds of actions selected from the action of inhibiting keratinocyte integrin production; The whitening agent of Claim 12 which has 2 or more types.   A skin external preparation containing the whitening agent according to claim 12 or 13.