JP2014009089A - Low temperature storage system - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a low temperature storage system which at least suppresses a temperature rise of a sample stored in a container held by a storage plate after taking out the storage plate from a storage rack, and prevents dew condensation and frost which tend to occur to the container and the storage plate.SOLUTION: A low temperature storage system 100 comprises: a plurality of storage racks 110 which accommodate a plurality of storage plates B; a low temperature storage chamber 120 for storing the plurality of storage racks 110; a working chamber 130 arranged adjacent to the low temperature chamber 120; a plate conveying mechanism 140 which puts in and takes out a plate B to/from a storage rack 110; a pickup mechanism 150 configured in such a manner that it is capable of taking out each container C individually; and a low temperature accommodating tank 160 which is attached to the plate conveying mechanism 140 and accommodates the storage plates B; and provides a low dew-point temperature environment for the containers C and the storage plates B.

Description

  The present invention relates to a cryogenic storage system for storing a container containing a sample at a low temperature, and particularly used in drug discovery, that is, a process for discovering and designing a drug in medicine, biotechnology and pharmacy. The present invention relates to a cryogenic storage system used for storing a drug discovery sample at an ultra-low temperature.

In the field of drug discovery research, it is necessary to conduct experiments for storing and analyzing large quantities of samples at low temperatures with high efficiency.
For this reason, a plurality of storage plates, each holding a plurality of containers for storing samples to be stored at low temperatures, are vertically stacked on the storage rack and stored individually and automatically from the target storage plate as necessary. A low-temperature storage system for taking out has been proposed (see, for example, Patent Document 1).

  The low-temperature storage system described above is provided with a plurality of storage racks that accommodate a plurality of storage plates stacked in the vertical direction, a low-temperature storage chamber that stores the plurality of storage racks, and an upper side of the low-temperature storage chamber. A working chamber, a plate conveying mechanism that is provided in the working chamber and conveys the storage plate and puts it in and out of the storage rack, and a storage plate and a plate conveyance that are conveyed by the plate conveying mechanism from an input / output chamber provided adjacent to the working chamber. And a pickup mechanism configured to be able to individually transfer containers to and from the storage plate taken out from the storage rack by the mechanism.

JP 2012-56730 A (all pages, all figures)

  However, in the above-described low-temperature storage system, the temperature of the working chamber is not as low as the temperature of the low-temperature storage chamber, so after the storage plate is taken out from the storage rack, it is taken out from the container or storage plate held on this storage plate. There was a problem that the containers were exposed to a temperature higher than the temperature of the cold storage chamber, and the temperature in the samples in these containers increased.

  In addition, it is possible to reduce the effect on the sample in the container by setting the cryogenic storage chamber to an ultra-low temperature state and reducing the exposure time to the external environment for a short period of time. There was a problem that a large amount of dew condensation and frost were generated in the container.

  Therefore, the technical problem to be solved by the present invention, that is, the object of the present invention is to increase the temperature of the sample contained in at least the container held on the storage plate after taking out the storage plate from the storage rack. Another object of the present invention is to provide a low-temperature storage system that suppresses condensation and frost that tend to occur on containers and storage plates while realizing a low dew point environment.

  According to the first aspect of the present invention, there are provided a plurality of storage racks that respectively accommodate a plurality of storage plates that respectively hold a plurality of containers in the vertical direction, a low-temperature storage chamber that stores the plurality of storage racks, and the low-temperature storage chamber A work chamber provided adjacent to the work chamber, a plate transport mechanism that is provided in the work chamber and transports the storage plate and puts it in and out of the storage rack, and a pickup mechanism configured to be able to take out the containers individually. In the low-temperature storage system provided, a low-temperature storage tank that stores the storage plate is attached to the plate transport mechanism, thereby solving the above-described problems.

  In the invention according to claim 2, in addition to the configuration of the low-temperature storage system according to claim 1, the pickup mechanism is transported by the plate transport mechanism from an input / output chamber provided adjacent to the work chamber. The container is attached to the plate transport mechanism in a state in which the container can be individually transferred in the low temperature storage tank between the storage plate and the storage plate taken out from the storage rack by the plate transport mechanism. This solves the above-mentioned problems.

  According to the third aspect of the present invention, in addition to the configuration of the low temperature storage system according to the first or second aspect, the plate transport mechanism is configured to allow the storage rack to be moved up and down individually from the low temperature storage chamber to the work chamber. Storage rack lifting and lowering means, storage plate pulling means for individually taking in and out the storage plate from the storage rack, and transport configured to be movable in the work chamber in a state where the storage rack lifting and lowering means and the storage plate pulling means are installed. The above-described problems are further solved by having the moving means.

  In the invention according to claim 4, in addition to the configuration of the low temperature storage system according to any one of claims 1 to 3, the low temperature storage chamber is maintained in an ultra low temperature environment of −80 ° C. or lower. Since the working chamber is maintained in a low humidity environment, the above-described problems are further solved.

A cryogenic storage system according to the invention of claim 1 includes a plurality of storage racks that respectively accommodate a plurality of storage plates that respectively hold a plurality of containers in the vertical direction, a cryogenic storage chamber that stores the plurality of storage racks, A working chamber provided adjacent to the low-temperature storage chamber, a plate conveying mechanism provided in the working chamber for conveying the storage plate and taking it in and out of the storage rack, and a pickup mechanism configured to be able to take out the containers individually In addition to being able to transfer containers individually and automatically from the storage plate, the following effects specific to the present invention can be achieved.
That is, in the low temperature storage system according to the invention of claim 1, the low temperature storage tank for storing the storage plate is attached to the plate transport mechanism, so that the storage plate stored in the low temperature storage tank at a lower temperature than the work chamber. In addition, the temperature rise of the container in the storage plate is suppressed, and the temperature difference between the storage plate, the container, and the low temperature storage tank is smaller than that of the storage plate, the container, and the working chamber. While suppressing the rise, a low dew point environment can be realized to prevent dew condensation and frost that tend to occur on containers and storage plates.

According to the low-temperature storage system according to the second aspect, in addition to the effect exhibited by the low-temperature storage system according to the first aspect, the pickup mechanism is transported by the plate transport mechanism from the loading / unloading chamber provided adjacent to the work chamber. By being attached to the plate transport mechanism in a state where the container can be individually transferred in the low temperature storage tank between the storage plate and the storage plate taken out from the storage rack by the plate transport mechanism, Since the storage plate and the container in the storage plate are in a low temperature environment lower than the work room, not only during the transfer of the storage plate but also during the transfer of the container, the temperature rise of the sample during the transfer of the container is suppressed and the container Condensation and frost that tend to occur in containers and storage plates during transfer can be prevented.
In addition, compared to the case where the pickup mechanism is spaced apart from the plate transport mechanism, the transport distance for transporting the storage plate taken out from the storage rack to the pickup mechanism is shortened, so that the storage plate is removed from the storage rack and stored again. Since the time until it is stored in the rack is shortened, the time during which the storage rack and the storage plate stored in the storage rack are carried out of the low temperature storage chamber is shortened to reduce the chance of condensation or frost, that is, the time. Can do.
Furthermore, since the plate mounting portion provided in the pickup mechanism for mounting the storage plate is locally cooled in the low temperature storage tank, the container and the storage plate that have just come out of the low temperature storage chamber when the container is transferred and these Condensation and frost can be prevented by making it difficult to increase the temperature difference from the surrounding space.

  According to the low temperature storage system according to the third aspect, in addition to the effect exhibited by the low temperature storage system according to the first or second aspect, the plate transport mechanism lifts the storage rack individually from the low temperature storage room to the work room. Storage rack lifting and lowering means that can be freely inserted and removed, storage plate pulling means for individually loading and unloading storage plates from the storage rack, and transport configured to be movable in the work chamber with the storage rack lifting and lowering means and the storage plate pulling means installed For moving the storage plate taken out from the low temperature storage chamber in the vertical and horizontal directions and transporting it in the work chamber quickly, so that the storage plate is taken out from the low temperature storage chamber. It is possible to suppress the temperature rise of the sample by shortening the plate transport time that leads to the temperature rise of the storage plate and the container in this storage plate. That.

  According to the cryogenic storage system according to claim 4, in addition to the effects exhibited by the cryogenic storage system according to any one of claims 1 to 3, the cryogenic storage chamber is placed in an ultra-low temperature environment of −80 ° C. or lower. Because the work chamber is kept in a low-humidity environment, the storage plate and the container even if the container held on the storage plate is taken out into the work chamber at an extremely low temperature of −80 ° C. or lower. Since the amount of water vapor condensed on the surface of the container is small, the amount of condensation or frost that can occur in the container or the storage plate can be reduced to an extent that does not adversely affect the storage state of the sample in the container.

BRIEF DESCRIPTION OF THE DRAWINGS Whole side explanatory drawing of the low-temperature storage system of this invention. FIG. 2 is an overall plan view of the low-temperature storage system of FIG. 1. The perspective view of the container and storage plate stored in the low-temperature storage system of FIG. Operation | movement explanatory drawing at the time of carrying in the empty storage plate of the low-temperature storage system of FIG. Operation | movement explanatory drawing at the time of the empty storage plate movement of the low-temperature storage system of FIG. Operation | movement explanatory drawing at the time of taking out the storage plate stored in the low-temperature storage system of FIG. Operation | movement explanatory drawing at the time of the storage plate movement stored in the low-temperature storage system of FIG. The whole top view of the cryogenic storage system which concerns on the other Example of this invention. FIG. 9 is a partial perspective view of the cryogenic storage system of FIG. 8.

The present invention provides a plurality of storage racks that respectively store a plurality of storage plates that respectively hold a plurality of containers in a vertical direction, a low-temperature storage chamber that stores the plurality of storage racks, and an adjacent to the low-temperature storage chamber. In a low-temperature storage system comprising: a work chamber provided; a plate transport mechanism that is provided in the work chamber and transports a storage plate and takes it in and out of a storage rack; and a pickup mechanism configured to be able to individually take out containers A low-temperature storage tank for storing the storage plate is attached to the plate transport mechanism to suppress the temperature rise of the sample stored in the container and to reduce the temperature difference between the low dew point environment, that is, the storage plate and the container and the surrounding space. In order to prevent condensation and frost that tend to occur on containers and storage plates in an environment that suppresses the formation of condensation and frost. As long as, manner of its specific implementation, may any be any one.
Moreover, in the low-temperature storage system of this invention, operation | movement of a system is controlled by a control apparatus so that a container can be taken out separately.
In each of the following embodiments, when it is necessary to distinguish a plurality of storage plates from each other for convenience of explanation, the reference symbol B is distinguished from each other by adding subscripts 1 and 2.

First, an embodiment of the low-temperature storage system of the present invention will be described with reference to FIGS.
As shown in FIGS. 1 to 3, a low temperature storage system 100 according to an embodiment of the present invention includes a plurality of storage plates B that respectively hold a plurality of containers C and accommodate a plurality of storage plates B stacked in the vertical direction, that is, in the vertical direction. Storage rack 110, a low temperature storage chamber 120 for storing a plurality of storage racks 110, a work chamber 130 provided adjacent to the low temperature storage chamber 120, and a storage plate B provided in the work chamber 130. A plate transport mechanism 140 for taking in and out of the storage rack 110 and a pickup mechanism 150 configured to be able to take out the containers C individually are provided.

Here, the plate transport mechanism 140 described above includes a storage rack elevating mechanism 141 that allows an arbitrary storage rack 110 among the plurality of storage racks 110 stored in the low temperature storage chamber 120 to be moved up and down individually, and a storage rack 110. A storage plate pull-out mechanism 142 that pulls out and puts out an arbitrary storage plate B out of the stored storage plates B from the storage rack 110 that is pulled out individually in the horizontal direction, a longitudinally moving slide portion 143A, and a lateral movement And a transfer moving body 143 including a slide portion 143B.
In this embodiment, the storage rack lifting / lowering mechanism 141, the storage plate pulling mechanism 142, and the transport moving body 143 are the "storage rack lifting / lowering means", "storage rack pulling means", and "transport moving means" in the present invention. It is an example.

Further, the storage rack elevating mechanism 141 and the storage plate pulling mechanism 142 are supported by a vertically moving slide portion 143A that is movable in the horizontal front-rear direction. The vertically moving slide portion 143A is supported by a horizontally moving slide portion 143B configured to be movable on the rail R along the horizontal right and left direction perpendicular to the moving direction of the vertically moving slide portion 143A, that is, in the horizontal direction. ing.
Further, the plate transport mechanism 140 is configured to move in the working chamber 130 in accordance with the movement of the longitudinally moving slide portion 143A and the laterally moving slide portion 143B.
The storage plate drawing mechanism 142 is configured to be able to transfer the storage plate B to the plate mounting portion 152 and the plate turning table 180 at a position facing the loading / unloading chamber 170 and a position facing the pickup mechanism 150. .

The pickup mechanism 150 is installed at a corner of the work chamber 130. Further, the pickup mechanism 150 has a plate placement portion 152 on which a plurality of storage plates B can be placed, and a grip portion that can move above the plate placement portion 152 and can individually take out and insert the container B. And a pickup arm 151.
For this reason, the pickup mechanism 150 can transfer the containers C individually and automatically between the plurality of storage plates B.

  The low-temperature storage system 100 according to the present embodiment places the loading / unloading chamber 170 provided at a position adjacent to the pickup mechanism 150 and the storage plate B carried in / out of the loading / unloading chamber 170 and the plate entrance / exit 190. And a plate turning table 180 for turning in a direction facing the plate conveying mechanism 140.

  As will be described later, the plate transport mechanism 140 is movable between a position above the low temperature storage chamber 120 where the storage rack lifting mechanism 141 operates, a position facing the loading / unloading chamber 170, and a position facing the pickup mechanism 150. It has become.

  As shown in FIG. 3, the storage plate B stores and stores a plurality of containers C in a matrix and is transported by the plate transport mechanism 140. On the other hand, the container C is a so-called drug discovery microtube, and is composed of a small and cylindrical container body C1 for storing a solution in which a sample is dissolved, and a cap C2 for sealing the solution in the container body C1. ing.

The carrying-out operation of the container B in the low temperature storage system 100 will be described with reference to FIGS.
As shown in FIG. 4, after the operator P places an empty storage plate B1 on the plate turning table 180 from the plate entrance / exit 190 on the side surface of the loading / unloading chamber 170 and closes the plate entrance / exit 190, a control (not shown) is performed. Give start instructions to the device. Then, the empty storage plate B <b> 1 carried in from the plate entrance / exit 190 and placed on the plate turning table 180 is directed to the side facing the plate transport mechanism 140 when the plate turning table 180 is turned. In this state, the storage plate pulling mechanism 142 of the plate transport mechanism 140 takes out the empty storage plate B1 from the plate turning table 180.

  Subsequently, as shown in FIG. 5, the storage plate pulling mechanism 142 transfers the empty storage plate B <b> 1 to the plate mounting portion 152 of the pickup mechanism 150.

  Subsequently, as shown in FIG. 6, the plate transport mechanism 140 moves to the position of the storage rack 110 in which the storage plate B2 to be taken out is accommodated. Here, the storage rack lifting mechanism 141 pulls the storage rack 110 upward from the low temperature storage chamber 120. At that time, the storage plate drawer mechanism 142 takes out the storage plate B2 containing the container C to be taken out from the storage rack 110.

  Subsequently, as shown in FIG. 7, the plate transport mechanism 140 moves, and the storage plate B <b> 2 that accommodates the container C to be taken out is transferred to the plate mounting portion 152 of the pickup mechanism 150 by the storage plate drawing mechanism 142. Then, the pickup arm 151 moves above the plate mounting unit 152 in a state where the empty storage plate B1 and the storage plate B2 that accommodates the container C to be taken out are mounted on the plate mounting unit 152 of the pickup mechanism 150. Then, the target container C is picked up from the storage plate B2 containing the container C to be taken out and transferred to the empty storage plate B1.

When the transfer of the container C to be taken out to the empty storage plate B1 is completed, the storage plate B1 on which the container C to be taken out is transferred is transported to the loading / unloading chamber 170 in the reverse order of the above-described operation.
That is, the storage plate B2 in which only the container C to be taken out is picked up is taken out again from the plate mounting portion 152 of the pickup mechanism 150 to the storage plate pulling mechanism 142 as shown in FIG. It is conveyed to position 110.

  Then, the storage rack elevating mechanism 141 pulls the storage rack 110 upward from the low temperature storage chamber 120, and the storage plate B 2 is accommodated in the storage rack 110 by the storage plate pulling mechanism 142. Thereafter, the storage rack 110 containing the storage plate B2 is stored in the low temperature storage chamber 120 again.

Subsequently, the storage plate B <b> 1 on which the container C to be taken out is transferred is taken out from the plate placement unit 152 of the pickup mechanism 150 by the storage plate drawing mechanism 142 and transferred to the plate turning table 180. Then, by rotating the plate turning table 180, the storage plate B1 is directed toward the plate entrance / exit 190, and the operator P can take out from the plate entrance / exit 190 on the side surface of the loading / unloading chamber 170.
By operating as described above, the low temperature storage system 100 transfers the containers C individually and automatically from the storage plates B2 without carrying out the storage plates B2 containing the containers C to be taken out from the work chamber 106. It can be taken out.

Next, a specific form of the low-temperature storage tank 160 that is the most characteristic of the low-temperature storage system 100 of the present embodiment will be described with reference to FIGS. 1 to 7.
As shown in FIGS. 1 to 7, in the low-temperature storage system 100, the low-temperature storage tank 160 that stores the storage plate B is attached to the plate transport mechanism 140, so that the low-temperature storage tank 160 is cooler than the work chamber 130. The rise of the temperature of the storage plate B and the container C in the storage plate B is suppressed, and the storage plate B, the container C, and the low temperature storage tank 160 are compared with the storage plate B, the container C, and the work chamber 130. The temperature difference becomes smaller. For this reason, while suppressing the temperature rise of the sample accommodated in the container C, the low dew point environment is implement | achieved and the dew condensation and the frost which tend to arise in the container C and the storage plate B are prevented.

  Further, in the low temperature storage system 100 of the present embodiment, the plate transport mechanism 140 stores the storage rack 110 from the storage rack 110 and the storage rack lifting mechanism 141 that allows the storage rack 110 to be moved up and down individually from the low temperature storage chamber 120 to the work chamber 130. A storage plate pulling mechanism 142 for individually loading and unloading the plate B; and a transfer moving body 143 configured to be movable in the work chamber 130 in a state where the storage rack lifting / lowering mechanism 141 and the storage plate pulling mechanism 142 are installed. Accordingly, the movement operation of the storage plate B taken out from the low temperature storage chamber 120 in the vertical direction and the horizontal direction and the transfer operation in the work chamber 130 are accelerated. For this reason, the plate conveyance time which leads to the temperature rise of the storage plate B taken out from the low temperature storage chamber 120 and the container C in this storage plate B is shortened, and the temperature rise of the sample is suppressed.

In the low temperature storage system 100 of the present embodiment, the low temperature storage chamber 120 is maintained in an ultra low temperature environment of −80 ° C. or lower, and the work chamber 130 is maintained in a low humidity environment, whereby the storage plate B and the storage plate B Even if the container C held in the container is taken out into the work chamber 130 in an ultra-low temperature state of −80 ° C. or lower, the water vapor condensed on the surfaces of the storage plate B and the container C is reduced. For this reason, the amount of condensation or frost that may occur in the container C or the storage plate B is reduced to such an extent that it does not adversely affect the storage state of the sample in the container C.
Moreover, the low humidity environment of the work chamber 130 may be maintained by providing a shutter between the work room 130 and the work room 130 so as to isolate the work room 130 from the work room 130.

  In the low temperature storage system 100 of the present embodiment obtained in this way, the low temperature storage tank 160 for storing the storage plate B is attached to the plate transport mechanism 140, so that the temperature rise of the sample stored in the container C is increased. In addition, the temperature rise of the storage plate can be suppressed, and condensation and frost that tend to occur in the container C and the storage plate B can be prevented in a state where a low dew point environment is realized.

Next, another embodiment of the cryogenic storage system according to the present invention will be described with reference to FIGS.
The low-temperature storage system 100A according to the present embodiment is different from the above-described low-temperature storage system 100 in that the pickup mechanism 150A is attached to the plate transport mechanism 140 and the container C is transferred or picked up in the low-temperature storage tank 160. The configuration is different. For this reason, common reference numerals are assigned to portions common to the cryogenic storage systems 100 and 200, and detailed descriptions thereof are omitted.

As shown in FIGS. 8 and 9, in the low temperature storage system 100 </ b> A according to the present embodiment, the pickup mechanism 150 </ b> A is stored by the plate transport mechanism 140 from the loading / unloading chamber 170 provided adjacent to the work chamber 130. The container C is attached to the plate transport mechanism 140 in such a state that the container C can be individually transferred in the low temperature storage tank 160 between the plate B1 and the storage plate B2 taken out from the storage rack 110 by the plate transport mechanism 140. Yes. That is, the pick-up mechanism 150A is configured so that the storage plate B can be interchanged with the storage plate drawing mechanism 142, and the pickup arm 151A that is movable in the vertical and horizontal directions in the figure. And the container C can be transferred between the plurality of storage plates B in the low-temperature storage tank 160.
In the low-temperature storage system 100A of the present embodiment thus obtained, the storage plate B and the container C in the storage plate B are transferred from the work chamber 130 not only during the transfer of the storage plate B but also during the transfer of the container C. Since it is in a low temperature environment, it suppresses the temperature rise of the sample during container transfer and prevents condensation and frost that may occur in the container C and storage plate B in a state where a low dew point environment is realized during container transfer. .

  In addition, as compared with the case where the pickup mechanism 150A is spaced apart from the plate transport mechanism 140, the transport distance for transporting the storage plate B taken out from the storage rack 110 to the pickup mechanism 150A is shortened, so that the storage plate B is stored. The time from taking out the rack 110 and storing it again in the storage rack 110 is shortened. For this reason, the time when the storage plate 110 stored in the storage rack 110 and the storage rack 110 is carried out of the low temperature storage chamber 120 is shortened, and the chances of dew condensation and frost are reduced.

At this time, the plate mounting portion 152A provided in the pickup mechanism 150A and mounting the storage plate B is locally cooled. For this reason, it is difficult to increase the temperature difference between the container C and the storage plate B that have just come out of the low temperature storage chamber 120 and their surrounding space when the container is transferred, thereby preventing condensation and frost.
In addition, the plate mounting portion 151A of the pickup mechanism 150A reduces the amount of condensation and frost that can be generated in the container C and the storage plate B when kept in an ultra-low temperature environment of −80 ° C. or lower as in the low temperature storage chamber 120. It is more preferable in reducing to a level that does not adversely affect the storage state of the sample in the container C.

100, 100A ... Low temperature storage system 110 ... Storage rack 120 ... Low temperature storage chamber 130 ... Work chamber 140 ... Plate transport mechanism 141 ... Storage rack movement mechanism 142 ... Storage plate movement Mechanism 143... Transporting body 143A... Vertical movement slide portion 143B... Horizontal movement slide portion 150, 150A... Pickup mechanism 151, 151A... Pickup arm 152, 152A. Unit 160 ... Low temperature storage tank 170 ... Entry / exit chamber 180 ... Plate turning table 190 ... Plate doorway B, B1, B2 ... Storage plate C ... Container C1 ... Container body C2 ... Cap

Claims (4)

A plurality of storage racks that respectively accommodate a plurality of storage plates that respectively hold a plurality of containers in the vertical direction, a low-temperature storage chamber that stores the plurality of storage racks, and a work chamber provided adjacent to the low-temperature storage chamber A low-temperature storage system provided with a plate transport mechanism that is provided in the working chamber and transports the storage plate and puts it in and out of the storage rack; and a pickup mechanism configured to be able to take out the containers individually;
A low temperature storage system, wherein a low temperature storage tank for storing the storage plate is attached to the plate transport mechanism.   The container between the storage plate transported by the plate transport mechanism and the storage plate taken out from the storage rack by the plate transport mechanism from an entry / exit chamber provided adjacent to the work chamber. The low-temperature storage system according to claim 1, wherein the plate conveying mechanism is attached in a state that can be individually transferred in the low-temperature storage tank.   The plate transport mechanism includes a storage rack lifting / lowering means for moving the storage rack individually up and down from a low temperature storage room to a work room, a storage plate pulling means for individually loading / unloading the storage plate from the storage rack, and the storage The low-temperature storage system according to claim 1 or 2, further comprising a transfer moving means configured to be movable in the work chamber in a state where the rack elevating means and the storage plate pulling means are installed. . The low-temperature storage room is kept in an ultra-low temperature environment of −80 ° C. or lower,
The low temperature storage system according to any one of claims 1 to 3, wherein the work chamber is maintained in a low humidity environment.

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