JP2013534144A5 - - Google Patents

Description

FIG. 5 is a plot of oil release rate as a function of retention / mixing time for samples mixed in different ways including a vortex mixer , rotary mixer, stir bar, and ULTRA-TURRAX® disperser.

The ratio of oil phase to water phase can affect the PIT of the composition or emulsion. In general, PIT increases with increasing ratio of oil phase to water phase. Furthermore, the lower the surfactant concentration, the higher the rate of increase in PIT. In some embodiments, the ratio of oil phase to aqueous phase is about 1: 100 to about 100: 1, about 1:50 to about 50: 1 , about 1:20 to about 20: 1, about 1:10. To about 10: 1, about 1: 8 to about 8: 1, about 1: 6 to about 6: 1, about 1: 5 to about 5: 1, about 1: 4 to about 4: 1, about 1: 3 To about 3: 1, or from about 1: 2 to about 2: 1.

In another example, the purified composition can be final purified by the addition of a chelating agent to reduce the amount of metal in the composition. In certain embodiments, the purification step also includes removing metals present in the crude bioorganic composition by the addition of a chelating agent. Any suitable chelating agent can be used. Illustrative of suitable chelating agents, ascorbic acid, citric acid, maleic acid, oxalic acid, succinic acid, di-carboxymethyl glutamic acid, ethylenediamine disuccinic acid (EDDS), and the like ethylenediaminetetraacetic acid (EDTA).

Claims (15)

(a) providing a composition comprising a surfactant, a host cell, an aqueous medium, a bio-organic compound produced by the host cell, and an oil-in-water emulsion formed therefrom, comprising the step of: Wherein the solubility of the surfactant decreases with increasing temperature, and the temperature of the composition is at least about 1 ° C. lower than the phase inversion temperature of the composition;
(b) raising the temperature of the oil-in-water emulsion to a temperature at least about 1 ° C. above the phase inversion temperature, thereby converting the oil-in-water emulsion to a water-in-oil emulsion; and
(c) A method comprising liquid-liquid separation of the composition to provide a crude bioorganic composition.   The method of claim 1, wherein the composition is an oil-in-water emulsion.   The method further comprising the step of reducing the amount of the composition prior to step (b), wherein substantially all of the bioorganic compound remains in the composition. The method described.   4. The method of claim 3, wherein the amount of the composition is reduced by about 75% or more.   The temperature in step (a) is at least about 5 ° C. or at least about 10 ° C. lower than the phase inversion temperature, and the temperature in step (b) is at least about 5 ° C. or at least about at least about the phase inversion temperature. 5. A method according to any one of claims 1 to 4 wherein the temperature is increased to 10C or at least about 15C higher.   6. The method according to any one of claims 1 to 5, further comprising the step of purifying the crude bioorganic composition to obtain a purified bioorganic composition.   7. The method of claim 6, wherein the purification of the crude bioorganic composition is by flash distillation.   7. The method of claim 6, further comprising treating the purified bioorganic composition with an antioxidant or a phenolic antioxidant.   A composition comprising a surfactant, a host cell, an aqueous medium, and a bioorganic compound produced by the host cell, wherein the solubility of the surfactant in the aqueous medium decreases with increasing temperature, and the composition The composition wherein the temperature of the product is at least about 1 ° C. higher than the phase inversion temperature of the composition and the bioorganic compound is an isoprenoid. The surfactant comprises a nonionic surfactant, or the surfactant is a polyether polyol, polyoxyethylene C _8-20 -alkyl ether, polyoxyethylene C _8-20 -alkyl aryl ether, polyoxy Ethylene C _8-20 -alkylamine, polyoxyethylene C _8-20 -alkenyl ether, polyoxyethylene C _8-20 -alkenylamine, polyethylene glycol alkyl ether, or combinations thereof; or polyether polyol, polyoxyethylene nonyl 9. The composition of claim 9 or the method of any one of claims 1-8, comprising phenyl ether, polyoxyethylene dodecyl phenyl ether, or a combination thereof.   11. The composition of any one of claims 9 to 10, wherein the temperature of the composition is at least about 5 ° C, at least about 10 ° C, or at least about 15 ° C higher than the phase inversion temperature.   The composition according to any one of claims 9 to 10, wherein the bioorganic compound is a hydrocarbon, isoprenoid, or farnesene, and the farnesene is α-farnesene, β-farnesene, or a combination thereof. Or a process according to any one of claims 1-8.   The composition according to any one of claims 9 to 12, or the method according to any one of claims 1 to 8, wherein the host cell is a bacterium, a fungus, an algae, or a combination thereof.   The host cell is Escherichia, Bacillus, Lactobacillus, Criveromyces, Pichia, Saccharomyces, Yarrowia, Saccharomyces cerevisiae, Chlorella Minutissima, Chlorella emazoni, Chlorella solokiniana, Chlorella ellipsoida, 13. A composition according to any one of claims 9-12 or a method according to any one of claims 1-8, selected from species, Chlorella protosequoides, and combinations thereof.   15. The composition according to any one of claims 9 to 14, wherein the composition is an emulsion.

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