JP2013215189A - Nonpathogenic xanthomonas strain and microbial pesticide using the strain - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a nonpathogenic bacterial strain exhibiting stable plant disease-controlling effects even in an actual crop-producing site, also exhibiting effects except the plant disease control, and safely usable as a microbial pesticide and the like, and to provide a new plant disease-controlling agent and the like using the strain.SOLUTION: There are provided an agent for controlling bacterial plant disease such as an agent for controlling black rot of Cruciferae, an agent for controlling bacterial spot of Solanaceae, an agent for controlling bacterial spot of lettuce, an agent for controlling bacterial shot hole of peach and an agent for controlling cankar of citrus fruits, a pest controller such as agents for controlling aphids and/or spider mites, a plant growth accelerator such as a promoter for promoting seed germination of goosefoot family plants, and the like, containing one or more microbial cells selected from two strains of Xanthomonas sp. 11-100-01 strain (NITE P-1243) and Xanthomonas sp. 11-110-01 strain (NITE P-1244) and/or a cultured product of the microbial cells as active ingredients.

Description

  The present invention relates to a novel non-pathogenic Xanthomonas bacterium strain, a plant disease control agent, a pest control agent, a plant growth regulator (plant growth promoter) and the like using the strain.

  In the prevention of crop diseases (plant diseases) caused by pathogenic bacteria, copper agents and antibiotic agents are used prophylactically. However, the use of large amounts of antibiotic agents is associated with the risk of appearance of drug-resistant bacteria. Is limited. In addition, the use of a large amount of copper agent may promote the appearance of copper agent-resistant bacteria and may cause phytotoxicity such as fouling in the product. And when the usage-amounts, such as a copper agent and an antibiotic agent, are restrict | limited, the scene which cannot acquire sufficient control effect can also be seen.

  For this reason, the development of a new drug (plant disease control agent) to replace copper and antibiotics has been desired for some time. Among these, development of microbial pesticides using non-pathogenic microorganisms has attracted attention in recent years because of its safety.

  Biokeepers (Central Glass Co., Ltd. products: registered trademark and patent document), which are soft rot control agents using non-pathogenic Erwinia carotovora bacteria, have been successfully used as microbial pesticides against bacterial plant diseases. 1) etc.

  However, most conventional microbial pesticides are basically limited to specific diseases that are subject to control, and are therefore less versatile, and all development and manufacturing costs are reflected in the selling price. For this reason, it is the present situation that only drugs targeting crops with high economic advantages can be developed.

  Therefore, the development of microbial pesticides that are useful as comprehensive control agents for plant diseases that are not economically affected by multiple plant diseases, but also that exhibit effects other than plant disease control. However, there is a strong demand in the industry. In particular, various types of plant diseases caused by pathogenic Xanthomonas bacteria have occurred, and there is a great deal of economic damage. There is a strong demand for the development of control agents.

Japanese Patent Laid-Open No. 10-327849

  The present invention provides a stable plant disease control effect at an actual crop production site, and exhibits an effect other than plant disease control. This was made for the purpose of providing a novel plant disease control agent used.

  In order to achieve the above object, as a result of intensive studies from various directions, the present inventors have found that two new strains belonging to non-pathogenic Xanthomonas exhibit remarkable effects in controlling various plant diseases. In addition, the present inventors have found that the present invention has also demonstrated pest control effects and plant growth regulation effects, and can be used safely in actual crop production sites.

That is, the embodiment of the present invention is as follows.
(1) Xanthomonas sp. 11-100-01 strain (NITE P-1243).
(2) Xanthomonas sp. 11-110-01 strain (NITE P-1244).
(3) One or more bacterial cells selected from the 11-100-01 strain described in (1) and the 11-110-01 strain described in (2) and / or a culture of the bacterial cells are contained as active ingredients. A plant disease control agent characterized by that.
(4) The agent according to (3), which is a bacterial plant disease control agent.
(5) The agent according to (4), which is a cruciferous control agent (eg, cabbage, cabbage, radish, turnip, broccoli, cauliflower, chingensai).
(6) The agent according to (4), wherein the agent is an eggplant family (eggplant, tomato, pepper, etc.) spot bacterial disease control agent.
(7) The agent according to (4), which is a lettuce spot bacterial disease control agent.
(8) The agent according to (4), which is a peach perforated bacterial disease control agent.
(9) The agent according to (4), which is a citrus (oranges, grapefruits, mandarin oranges, perfumed citrus fruits, etc.) scab control agent.
(10) One or more cells selected from the 11-100-01 strain described in (1) and the 11-110-01 strain described in (2) and / or a culture of the cells are contained as active ingredients. A pest control agent characterized by that.
(11) The agent according to (10), which is a control agent for aphids (eg, cotton aphids) and / or spider mites (eg, spider mites).
(12) One or more cells selected from the 11-100-01 strain described in (1) and the 11-110-01 strain described in (2) and / or a culture of the cells are contained as active ingredients. An agent for regulating plant growth (promoting plant growth).
(13) The agent according to (12), which is a seed germination promoter of a red crustacean crop (such as spinach, sugar beet, quinoa, red akaza).

  According to the present invention, one or more cells selected from two novel strains (11-100-01 strain and / or 11-110-01 strain) belonging to non-pathogenic Xanthomonas and / or the strain By using body culture as an active ingredient, it is effective for controlling many types of plant diseases, etc., and is a comprehensive plant disease control agent and pest control agent that can be used safely in actual crop production sites. A plant growth regulator and the like can be provided.

  In the present invention, new strains 11-100-01 and 11-110-01, which are non-pathogenic and belong to Xanthomonas, are used. Both the 11-100-01 strain and the 11-110-01 strain have bacteriological properties as shown below.

(A) Characteristics of morphology (1) Cell size: 0.4 to 0.7 μm × 0.7 to 2.0 μm
(2) Cell shape: cocoon (3) Motility: Existence (4) Gram staining: Negative (b) Physiological and chemical taxonomic properties (1) Optimal growth conditions (temperature, pH): 28-30 ° C , Neutral zone (2) Anaerobic growth: None (3) OF test: O
(4) Nitrate availability: negative (5) Organic acid availability: acetic acid, citric acid, propionic acid, succinic acid, lactic acid, D-tartaric acid can be used (6) carbon source utilization: L-arabinose, Producing acids from D-glucose, sucrose, D-mannose, galactose, trehalose, D-cellobiose, D-fructose, maltose, D-ribose, dextrin, glycerin, α-methylglucoside, D-raffinose, D-melezitose, Does not produce acid from D-sorbitol, D-galactitol, meso-inositol, inulin (7) Levan production: +
(8) Growth in the presence of 4% NaCl: +
(9) Growth on nutrient agar medium: Good (10) Starch hydrolysis: +
(11) Gelatin hydrolysis: +
(12) Esclin hydrolysis: +
(13) Milk digestion: +
(14) H ₂ S production: +
(15) Catalase activity: +
(16) Lecithinase activity: +
(17) Phytopathogenicity: green beans, soybeans, cucumbers, bitterns, cucumbers, cabbage, Chinese cabbage, turnips, Japanese radish, Japanese mustard spinach, cauliflower, mustard greens, tomatoes, peppers, eggplant, lettuce, red clover, sunflower, Italian ryegrass, perennial Ryegrass, oats, orchardgrass, sorghum, corn, rice, millet millet, white-nosed grasshopper are non-pathogenic by needle inoculation

  These 11-100-01 strain and 11-110-01 strain are respectively incorporated by the National Institute of Technology and Evaluation Microbiology Depositary Center (2-8-8 Kazusa Kamashi, Kisarazu City, Chiba Prefecture, Japan 292-0818). ) As of February 21, 2012, and the accession numbers are NITE P-1243 and NITE P-1244, respectively.

  As for any of the 11-100-01 strain and the 11-110-01 strain, any medium may be used as long as the strain can grow. For example, as the carbon source used in the medium, glucose, starch, starch saccharified solution, sugars such as molasses, organic acids such as citric acid, and the like are appropriately used. In addition, inorganic salts and trace organic nutrients (vitamins, amino acids, nucleic acid-related substances, etc.) may be added, or organic substances such as peptone, meat extract, yeast extract, and soybean meal may be added. Furthermore, various additives such as an antifoaming agent can be added as necessary.

  The cultures of the 11-100-01 strain and the 11-110-01 strain can be cultured by a liquid culture method or a solid culture method under aerobic conditions. The culture conditions are not limited to this, but the temperature is preferably 25-30 ° C. and the pH is neutral.

  The 11-100-01 and 11-110-01 strains cultured as described above can be used as active ingredients such as plant disease control agents in the state of the culture without being separated from the culture. Moreover, each said microbial cell is isolate | separated from the said culture by a normal method, for example, processes, such as membrane separation or centrifugation, and it can also utilize in the state of a microbial cell. Furthermore, it can also be used in the state of a dried product obtained by drying the above-mentioned culture or separated bacterial cells by a method such as freeze drying or spray drying. Moreover, it can also be used in the state formulated into various dosage forms together with various additives according to conventional methods for agricultural chemical formulations. Examples of such dosage forms include granules, emulsions, wettable powders and flowable agents.

The plant disease control agent and the like according to the present invention may contain 11-100-01 strain or 11-110-01 strain alone, or contain these strains in combination at any ratio. It may be a thing. The concentration of bacteria contained in the drug is not particularly limited as long as the desired effect is exhibited. For example, the total number of bacteria of the strain per 100 g of drug is 1 × 10 ⁵ to 1 × 10 ¹⁴ , preferably 1 × 10 ⁷ to Examples are those containing 1 × 10 ^13, more preferably 1 × 10 ⁸ to 1 × 10 ¹¹ . Even if it is prepared and used as a liquid agent, the concentration of bacteria contained in it is not limited as long as the desired effect can be obtained. However, if the concentration of bacteria is too low, sufficient results cannot be obtained. On the contrary, since the bacteria may be wasted even if the bacteria concentration is increased too much, for example, it can be appropriately adjusted in the range of 1 × 10 ³ to 1 × 10 ¹² cfu / ml, and preferably 1 × 10 6 ^{A range of 5} to 1 × 10 ¹¹ cfu / ml, more desirably 1 × 10 ⁶ to 1 × 10 ⁹ cfu / ml is preferable.

  The present invention is not particularly limited, but various plant diseases such as various bacterial plant diseases caused by pathogenic Xanthomonas bacteria (Brassicaceae (such as cabbage, cabbage, radish, turnip, broccoli, cauliflower, tingensai, komatsuna, mizuna) Widely suitable for black rot, solanaceous (such as eggplant, tomato, pepper) spot bacterial disease, lettuce spot bacterial disease, citrus (orange, grapefruit, mandarin orange, citrus citrus, etc.) Exhibits a good control effect. Further, it is also characterized in that it exhibits a suitable control effect against bacterial plant diseases (for example, peach perforated bacterial diseases) involving pathogenic bacteria other than pathogenic Xanthomonas bacteria.

Further, the present invention is not particularly limited, but it exerts a wide and favorable control effect against various plant pests such as aphids (cotton aphids, peach aphids, etc.), spider mites (spider spider mites, Kanzawa spider mites, etc.) and the like.
In the present invention, “control” refers to avoiding plant diseases and the like by preventing the crops (plants) from being infected with the target plant pests or the target pests being infested. Means.

  Furthermore, the present invention is not particularly limited, but also exerts effects such as plant growth regulation, for example, germination promoting action of seeds of red crustacean crops (such as spinach, sugar beet, quinoa, red akaza).

  The plant disease control agent etc. which concern on this invention can be applied directly as it is, or can be applied by diluting with water etc. The application method as a chemical | medical agent is not specifically limited, For example, the method of spraying to a crop directly, the method of spraying to soil, the method of adding to the water and fertilizer added to a crop or soil, etc. are mention | raise | lifted.

  The drug application rate of the present invention varies depending on the applied crop, target disease, application method, occurrence tendency, degree of damage, environmental conditions, dosage form to be used, etc. For example, 1 mL to 5 L, preferably 10 mL to 1 L of the liquid agent per crop is applied, and 0.5 to 1000 L of the liquid agent is applied per 10 ares. Also, the application time varies depending on the applied crop, dosage form, etc., but it is preferable to perform the treatment at about 2 to 4 weeks after sowing, and if it is a tree, it may be treated for about 1 to 2 years. Furthermore, since the present invention is a microbial pesticide that is free from the appearance of resistant bacteria, it can be used continuously for several days or continuously.

  Furthermore, the plant disease control agent and the like according to the present invention is an inorganic copper compound, for example, basic copper sulfate, anhydrous copper sulfate, cupric hydroxide, basic copper chloride, etc. within the range of use amount that does not cause phytotoxicity to plants. Organic copper compounds such as organic copper, copper nonylphenol sulfonate, inorganic sulfur compounds such as sulfur, total sulfurized sulfur, etc., organic sulfur compounds such as dinebu, mannebu, propineb, thiadiazine, thiuram, polycarbamate, anilinopyrimidine, etc. Compounds such as cyprodinil, pyrimethanil, mepanipyrim, phenylpyrrole compounds such as fludioxonil, organochlorine compounds such as chlorothalonil, captan, triazine, fluazinam, sulfenic acid, and fusalides, bicarbonate agents such as sodium bicarbonate, Organophosphorous compounds such as potassium bicarbonate EDDP, fosetyl, tolcrofosmethyl, IBP, etc., benzimidazole compounds such as carbendazim, thiophanate methyl, thiabendazole, benomyl, fuberidazole, etc., dicarboximide compounds such as iprodione, procymidone, vinclozoline, etc., azole compounds such as phen Buconazole, Cimeconazole, Diclobutrazole, Triticonazole, Ipconazole, Fluconazole, Microbutanyl, Penconazole, Viteltanol, Bromuconazole, Oxpoconazole, Cyproconazole, Difenoconazole, Diniconazole, Epoxyconazole, Fenbuconazole, Fluconazole Quinconazole, flusilazole, flutriazole, hexaconazole, imibenconazole, metco Sol, propiconazole, cypconazole, tebuconazole, tetraconazole, triadimethone, triazimenol, etc., imidazole compounds such as triflumizole, prochloraz, imazalyl, pefrazoate, piperazine compounds such as triphorin, morpholine compounds such as Fenpropimorph, tridemorph, fenpropidin, etc., hydroxypyrimidine compounds such as ethylimole, dimethylirimol, etc., guanidine compounds such as iminoctadine acetate, iminoctadine arbesylate, guazatine, etc., acid amide compounds such as oxycarboxin, etc. Benzoanilide compounds such as mepronil, dichromedin, flutolanil, pencyclon, furametopyl, tifluzamide, etc., acylalanine compounds such as , Oxazixyl, metalaxyl, methoxy acrylate compounds such as azoxystrobin, cresoxime methyl, metminostrobin, trifloxystrobin, picoxystrobin, pyraclostrobin, orissastrobin, quinoxaline compounds such as quinomethionate, hydroxyanilide Compounds such as phenhexamide, cyanoacetamide compounds such as simoxanil, cyanoimidazole compounds such as cyazofamide, etc.Famoxadone, spiroxamine, triazoxide, pyrazophos, fluorimide, dimethomorph, iprovaricarb, fenamidone, ethaboxam, cyflufenamide, dithianone, carpropamide , Probenazole, metasulfocarb, pyroxylone, hydroxyisoxy It tetrazole, tricyclazole, diflumetorim, Fenajinkishido, isoprothiolane, oxolinic acid, acibenzolar -S- methyl, quinoxyfen, benthiavalicarb-isopropyl, also be used in combination one or more chemical pesticides selected from triazinyl. In this case, it may be applied in the form of a mixture, or may be applied separately at a time, or both may be applied simultaneously.

  Thus, one or more cells selected from the new strain Xanthomonas sp. 11-100-01 and 11-110-01 and / or the cell culture are used as active ingredients. Show significant effects in controlling plant diseases such as cruciferous black rot, solanaceous spotted bacterial disease, lettuce spotted bacterial disease, peach perforated bacterial disease, citrus canker disease, and also pest control effect and plant growth promotion It is possible to provide a drug that can be used safely even in actual crop production sites, which is also effective.

  Examples of the present invention will be described below, but the present invention is not limited to these examples, and various modifications can be made within the technical idea of the present invention.

(Hakusai black rot onset suppression effect)
Chinese cabbage (variety: yellowish green) is sown in a black pot (diameter 13 cm), and the bacterial solution 1 × 10 ⁸ cfu of 11-100-01 strain and 11-110-01 strain is planted for about 3 weeks after sowing. / Ml (OD ₆₀₀ = 0.3 calculated as 2 × 10 ⁸ cfu / ml) was sprayed 10 ml per strain. Next day, spray 10 ml of causal bacteria solution of cabbage black rot (about 2 × 10 ⁵ cfu / ml) on the next day. It was evaluated by the spot area.

The test results are shown in Table 1. In both the 11-100-01 strain spray group and the 11-110-01 strain spray group, the diseased leaf rate and the lesion area were greatly reduced as compared to the untreated group. On the other hand, it was shown that a high control effect was exhibited.
In addition, the lower case letters in the disease leaf rate and lesion area data in Table 1 are not significantly different at the 5% level due to multiple comparison of the average values between the same signs in each item, and between the different signs. Indicates that there is a significant difference.

(Tomato spotted bacterial disease onset suppression effect)
Tomato (variety: Resina) is sown in a black pot (diameter 13 cm), and the bacterial solution of 11-100-01 strain, 11-110-01 strain is 1 × 10 ⁸ cfu / ml (about 3 weeks after sowing) OD ₆₀₀ = 0.3 was calculated as 2 × 10 ⁸ cfu / ml) and sprayed 10 ml per strain. The next day, tomato spot bacterial disease pathogenic bacteria solution (about 4 × 10 ⁵ cfu / ml) was sprayed 10 ml per strain, and on the 15th day after inoculation with pathogenic bacteria, the degree of disease was evaluated by the number of lesions compared to the untreated group did.

The test results are shown in Table 2. In both the 11-100-01 strain spray group and the 11-110-01 strain spray group, the number of lesions is greatly reduced compared to the untreated group, and these strains are also high against tomato spot bacterial disease. It was shown to exert a control effect.
The lower case letters in the data on the number of lesions in Table 2 indicate that there is no significant difference between the same signs at the 5% level due to multiple comparison of the average values, and there is a significant difference between the different signs.

(Inhibition effect of peach perforation bacterial disease)
Peach (variety: white birch, second grader, planted in No. 10 pot (diameter of about 30 cm)) 11-100-01 strain, 11-110-01 strain 5 × 10 ⁸ cfu / ml per strain 300 ml sprayed. Two days later, 1 L of peach perforated bacterial disease pathogenic bacterial solution (about 1 × 10 ⁸ cfu / ml) was sprayed per strain, and on the 20th day after inoculation with the pathogenic bacteria, the degree of pathogenesis and the disease leaf rate and The control value was evaluated. The control value was calculated by the following formula.

  Control value = 100 × (number of diseased leaves in untreated group−number of diseased leaves in treated group) / (number of diseased leaves in untreated group).

  The test results are shown in Table 3. In both the 11-100-01 spray group and the 11-110-01 spray group, the diseased leaf rate is greatly reduced as compared with the untreated group, that is, it shows a high control value. It was shown to exert a high control effect against bacterial diseases.

(Inhibition effect of citrus canker disease)
Navel orange (10 spring branches per ward, randomly selected from 30 trees in the field) 11-100-01 in NB liquid medium (27 ° C, 120 rpm, 2 days) once Sufficient amounts of each of the centrifugally washed suspensions 1 × 10 ⁷ (1E + 07) cells / ml and 1 × 10 ⁸ (1E + 08) cells / ml were dispersed by hand spray about every 3 weeks. As a control group, a commercially available antibiotic agent (validacin solution 5, Takeda Pharmaceutical Co., Ltd. product: registered trademark) and copper agent (Kosaide DF, Maruwa Biochemical Co., Ltd. product: registered trademark) were adjusted to a predetermined concentration. A thing was used. Two months after the first spraying, the severity of citrus canker was evaluated by the disease index, disease severity and control value compared to the control and untreated (tap water). In addition, the disease index, the disease severity, and the control value were investigated based on the following Japanese planting prevention survey criteria for spring branches and leaves.

<Disease index>
0: No lesion 1: 1: 1-3 lesions 3: 4-10 lesions 5: 11-20 lesions 7: 21 lesions or more

<Disease severity>
Disease severity = 100 × Σ (number of diseased leaves by index × index) / (number of leaves surveyed × 7)

<Control value>
Control value = 100 × (1− (average disease severity in treated area) / (average disease severity in untreated area))

  The test results are shown in Table 4. In the Kosid DF spraying zone, the control value was around 70 for both the 1000-fold dilution and the 2000-fold dilution. The control value was about 35 in the 5 spray area of validacin solution. On the other hand, in the non-pathogenic Xanthomonas sp. 11-100-01 spraying area, 1E + 07 and 1E + 08cells / ml show control values of 39.9 and 56.7, respectively, which are inferior to coricide DF, but validacin It was shown that there was an effect of controlling the scab of the same level as or higher than that of Solution 5.

(Lettuce spot bacterial disease onset suppression effect)
Lettuce (variety: red fire) is sown in a black pot (diameter 13 cm), and the bacterial solution of 11-100-01 and 11-110-01 strains is 1 × 10 ⁸ cfu / ml on the plant body for about 5 weeks after sowing. (Calculated as OD ₆₀₀ = 0.3 as 2 × 10 ⁸ cfu / ml) was sprayed 10 ml per strain. Two days later, 10 ml of lettuce spot bacterial disease liquid (about 1 × 10 ⁶ cfu / ml) was sprayed per strain, and on the 16th day after inoculation with the pathogenic bacteria, the degree of disease was evaluated by the number of lesions.

The test results are shown in Table 5. In both the 11-100-01 strain spray group and the 11-110-01 strain spray group, the number of lesions is greatly reduced compared to the untreated group, and these strains are also high against lettuce spot bacterial disease. It was shown to exert a control effect.
The lower case letters in the data on the number of lesions in Table 5 indicate that there is no significant difference between the same signs at the 5% level due to multiple comparison of the average values, and there is a significant difference between the different signs.

(Control effect of cotton aphids and spider mites)
The 11-100-01 and 11-110-01 strains were each inoculated into a 100 ml Erlenmeyer flask containing 20 ml of NB medium and cultured with shaking (27 ° C., 120 rpm, 18 hours). 1 ml of this culture solution was inoculated into a 500 ml Erlenmeyer flask containing 200 ml of NB medium, and cultured with shaking (27 ° C., 120 rpm, 24 hours). 0.02% of Kumiten (spreading agent, Kumiai Chemical Industry Co., Ltd.) was added to the culture solution to prepare a test bacterial solution. The number of viable bacteria in the test bacterial solution was 1E + 11 cfu / ml for the 11-100-01 strain and 5E + 10 cfu / ml for the 11-110-01 strain.

  As for cotton aphids (Aphis gossypii), 3 adults were inoculated into cucumber seedlings in the first true leaf development stage and allowed to lay at 25 ° C. (16 hours light / 8 hours dark) overnight. After removing the adults, the number of larvae was examined, and cucumber seedlings infested with 1st instar larvae were immersed in each test fungus solution for 5 seconds. Three days after the treatment, the number of live insects was examined, and the death rate was calculated.

  In addition, as for Tani ticks (Tetranychus urticae), 35 adult nymph mite were inoculated into soybeans at the initial leaf stage and immersed in each test bacterium solution for 5 seconds. It was left to stand at 25 ° C. (16 hours light / 8 hours dark), and the number of adult females was investigated 2 to 6 days after the treatment, and the death rate was calculated.

In all cases, the mortality rate was corrected by the following formula using the mortality rate data of the control (immersion group with only the spreading agent), and the corrected mortality rate was calculated.
Corrected mortality rate = {(mortality rate of treated area−0.02% Kumiten-added water mortality rate) / (100−0.02% Kumiten-added water mortality rate)} × 100

  The test results are shown in Table 6. Both the 11-100-01 strain immersion group and the 11-110-01 strain immersion group have a significantly increased death rate compared to the control, and these strains have a high control effect against cotton aphids and urticae. It was shown to work.

(Spinach seed germination promoting effect)
The 11-100-01 and 11-110-01 strains were each inoculated into a 100 ml Erlenmeyer flask containing 20 ml of NB medium and cultured with shaking (27 ° C., 120 rpm, 18 hours). 1 ml of this culture solution was inoculated into a 500 ml Erlenmeyer flask containing 200 ml of NB medium, and cultured with shaking (27 ° C., 120 rpm, 24 hours). After incubation, the cells were washed twice with sterile water by centrifugation (4 ° C., 4,000 g, 15 minutes).

  Then, the cultured strain was adjusted to a predetermined concentration (1E + 9 cfu / ml) with sterilized water, and spinach seeds (Spinia oleracea, variety: Jiromaru) were soaked for 24 hours (25 ° C.). After the soaking treatment, a filter paper moistened with distilled water was applied to the petri dish, and the soaked seeds were sown therein. Then, it stored at 20 degreeC and investigated the seed germination rate 4 days later.

The seed germination rate was calculated by the following formula.
Germination rate = (number of germinated seeds / number of seeds investigated) × 100

  The test results are shown in Table 7. Both the 11-100-01 strain immersion group and the 11-110-01 strain immersion group have an increased germination rate compared to the control (non-treated group), and in particular, germination of the 11-100-01 strain immersion group. The rate was greatly increased, indicating that these strains exerted spinach seed germination promoting effects.

As described above, the novel microorganisms 11-100-01 strain and 11-110-01 strain of the present invention all exhibit extremely excellent control effects against various plant diseases, and are comprehensive plant disease control agents. It has been shown that it can also be used as a pest and exerts a pest control action and a plant growth regulation action.
In addition, both the microorganisms 11-100-01 strain and 11-110-01 strain according to the present invention belong to non-pathogenic Xanthomonas that exist in nature, have excellent safety, and have an impact on the environment. Few.

  The present invention is summarized as follows.

  The present invention provides a stable plant disease control effect at an actual crop production site, and exhibits an effect other than plant disease control. It aims at providing the used novel plant disease control agent etc.

  And selected from two strains of Xanthomonas sp. 11-100-01 strain (NITE P-1243) and Xanthomonas sp. 11-110-01 strain (NITE P-1244) By using one or more cells and / or a culture of the cells as an active ingredient, the cruciferous black rot control agent, the solanaceous spot bacterial disease control agent, the lettuce spot bacterial disease control agent, the peach perforation bacterial disease control , Plant growth control agents such as citrus scab control agents, pest control agents such as aphids and / or spider mites control agents, plant growth regulators such as rhizome crop seed germination promoters, and all these effects It can provide comprehensive microbial pesticides that can also be used.

The accession numbers of the microorganisms deposited in the present invention are shown below.
(1) Xanthomonas sp. 11-100-01 strain (NITE P-1243).
(2) Xanthomonas sp. 11-110-01 strain (NITE P-1244).

Claims (13)

  Xanthomonas sp. 11-100-01 strain (NITE P-1243).   Xanthomonas sp. 11-110-01 strain (NITE P-1244).   It contains one or more bacterial cells selected from the 11-100-01 strain according to claim 1 and the 11-110-01 strain according to claim 2 and / or a culture of the bacterial cells as an active ingredient. A plant disease control agent.   The agent according to claim 3, which is a bacterial plant disease control agent.   The agent according to claim 4, which is a cruciferous black rot control agent.   The agent according to claim 4, which is a solanaceous spotted bacterial disease control agent.   The agent according to claim 4, which is a lettuce spot bacterial disease control agent.   The agent according to claim 4, which is a peach perforated bacterial disease control agent.   The agent according to claim 4, which is a citrus canker disease control agent.   It contains one or more bacterial cells selected from the 11-100-01 strain according to claim 1 and the 11-110-01 strain according to claim 2 and / or a culture of the bacterial cells as an active ingredient. A pest control agent.   The agent according to claim 10, which is an aphid and / or spider mite control agent.   It contains one or more bacterial cells selected from the 11-100-01 strain according to claim 1 and the 11-110-01 strain according to claim 2 and / or a culture of the bacterial cells as an active ingredient. Plant growth regulator.   The agent according to claim 12, wherein the agent is a rhesus crop seed germination promoter.