JP2013156268A - Measurement sample handling device having channel filled with liquid and sealed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a measurement sample handling device (1) which can be easily handled, including a handling unit (200) and a measurement device (10) for taking in a liquid sample.SOLUTION: A measurement device (10) has: a channel filled with a liquid prior to use; a measurement surface (20) for making contact with a liquid sample at use; and a plug portion (40) fittable into a socket of the measurement evaluation device and having an electric contact. A unit (200) has an opening (210) to which the measurement device (10) is inserted; an opening (220); and a sealing (34) for preventing the liquid leaking from the channel. After insertion of the measurement device (10), the opening (210) provides an access to the measurement surface (20), and the opening (220) provides an access to the electric contact. Both of the openings provide an electric contact seal for the unit (200) to seal the electric contact from the measurement surface (20).

Description

  The present invention relates to a sensor for components in a liquid sample. In particular, the present invention relates to a sensor for evaluating the concentration of charged species in a sample, such as blood, which can be easily handled by a user or patient, in particular an ion concentration, for example a lithium ion concentration.

  Inorganic ions are essential for life and are found in large quantities in drinking water, living blood and cells, and the environment. For example, the presence of many ions inside and outside the cell, such as sodium, potassium, magnesium, and calcium, is essential for living organisms. Consequently, the measurement of ion concentrations in the blood and blood cells of animals and humans is very important for various body functions.

  Normally, lithium is not present at all in plasma or merely as a trace element, but it is also used as a drug to treat bipolar mood disorders. It is estimated that over a million people around the world are taking lithium daily. The disadvantage with the use of lithium lies in a very low therapeutic index, ie the ratio between toxic and therapeutic concentrations. Most patients respond well to plasma with a lithium concentration of 0.4-1.2 mmol / L, while lithium concentrations above about 1.6 mmol / L are considered toxic. Long-term high blood lithium levels can cause permanent damage and even death to the nervous system. Therefore, it is essential to monitor the lithium concentration during treatment by keeping the lithium level at the desired level with periodic inspections every two months.

  The direct measurement of lithium in whole blood and the determination of inorganic cations in plasma are described in Electrophoresis 2004, 25, 1660-1667 and in Electrophoresis 2005, 26, 3032-3042. Described and demonstrated by Vrouwe et al. Using microchip capillary electrophoresis (CE) loaded with a given sample and applying the principle of column binding, the blood alkali metal concentration in a drop of whole blood was measured. Blood collected by finger puncture was transferred onto the chip without extraction or removal of components from the blood. The lithium concentration in the plasma of patients undergoing lithium treatment can be determined without sample pretreatment. Using a chip that conducts conductivity detection, a detection limit of 0.1 mmol / L was obtained for lithium in a 140 mmol / L sodium matrix.

  In these disclosures, the components of the blood sample are separated electrophoretically within the microchannel. A double T-injection configuration is used to select the ionic components of interest and direct them to the detection electrode.

  A method and apparatus for measuring ion concentration in a liquid sample is disclosed in co-pending PCT application PCT / EP2006 / 011148, the teachings of which are incorporated herein by reference. This PCT application describes an apparatus for the measurement of the concentration of charged species in a sample, the sample comprising a plurality of types of charged species and at least one insoluble component, the apparatus having at least a filter function At least one channel having one opening, at least two electrophoretic electrodes disposed along the at least one channel, and at least one for measuring at least one type of charged species in the at least one channel Includes two sensors.

  The dimensions of the openings and channels used in such devices are usually very small to reduce the amount of liquid required and the size of the device. Typical channel dimensions are on the order of a width of less than 1 cm and a depth of less than 100 μm. As a result, the device can even be made very small to minimize the amount of material used for the device. The material is often expensive, such as glass.

  The device should also be easily usable by the patient or other user. In particular, patients with bipolar mood disorders or similar illnesses often suffer from shaking or trembling hands and have problems handling small pieces.

  Furthermore, blood samples and consequently channels can be easily contaminated by blood or other liquids and cannot be reused without thorough cleaning and sterilization.

  In contrast, known conventional measurement devices are complex microfluidic and electronic components that are expensive and therefore not suitable for one-time use.

  It is an object of the present invention to provide a device and method for easy handling of a liquid sample measuring device for measuring a small amount of sample.

  It is a further object of the present invention to provide a measuring device that can be used as a disposable while using advanced measurement techniques.

  These and other objects of the present invention are achieved by a measurement sample handling device and method for taking a liquid sample according to the present invention, wherein the measurement sample handling device includes a measurement device and a handling unit.

  A measuring device for taking in a liquid sample comprises a measuring part having a measuring surface for contact with the liquid sample in use, a plug part having a plurality of electrical contacts and attachable to a socket of a measuring evaluation apparatus including. The measurement surface may be placed at a position on the measurement device that is different from the plug portion in order to avoid the liquid of the liquid sample from contacting the electrical contacts. In particular, the plug part may be arranged on the side of the measuring device that is different from the measuring surface.

  The measurement surface and the measurement part may be made from the same material, for example from glass, or may be realized as one piece. The measurement device may further include a plurality of electrodes coupled to the electrical contacts. The measuring device may not have any active electrical components such as switches, transistors or power sources. In some cases, the measurement device may include several passive electrical components such as a temperature sensor or the like.

  During assembly, the measurement device may be inserted into a handling unit, the handling unit having a first opening for the measurement surface and at least a second opening for the plurality of electrical contacts. Therefore, when inserted into the handling unit, the measurement surface is accessible by the user or patient to place the liquid sample. On the other hand, the plug part is accessible through a second opening of the handling unit to allow access to the electrical contacts, for example by a socket. Therefore, the handling unit may form an electrical contact seal to prevent liquid from coming into contact with electrical contacts in the plug portion of the measurement device.

  Since all electrical contacts are provided by the measuring device, the handling unit may not contain any electrical components. However, in some cases, the handling unit may include electrical contacts and electrical components.

  The handling unit may be substantially larger than the measuring device. Therefore, the handling unit can be reasonably sized for easy and safe handling of the measurement sample handling device, even by patients suffering from trembling hands or the like. At the same time, the measuring device can be kept small in order to minimize the amount of liquid sample required for reliable measurement. In addition, smaller measurement devices may be cheaper to make. The measurement sample handling device can therefore be a disposable device for several or one use. This is particularly useful when the liquid sample is a bodily fluid such as blood or another sample that requires a sterilized and / or cleaned environment.

  The handling unit may also be configured to accommodate multiple measurement devices for performing multiple sample measurements in series or in parallel. The measuring device also provides for easy access to the plug part from the outside in use, for example by means of a plurality of electrical pins, for example when the measuring sample handling device is inserted into the socket of the measuring evaluation device. As provided, it may be positioned on a particular side of the handling unit. The plurality of electrical pins may be configured to make electrical contact with the plurality of electrical contacts of the measurement device when the measurement device or measurement sample handling device is attached to the socket.

  A measurement evaluation device for evaluating at least one parameter of a liquid sample is a power source for the measurement device and all further electrical and electronic means for performing a measurement for evaluating the at least one parameter. May be included. In particular, the measurement evaluation device may include control means for controlling and monitoring the electrodes in the measurement device when the measurement device is inserted into the socket.

  The present invention also provides a step of placing a liquid sample on a measurement surface of a measurement device, the measurement device comprising a step having a plurality of electrical contacts, and at least some of the plurality of electrical pins comprising a plurality of electrical contacts. Assessing at least one parameter of the liquid sample comprising inserting a measuring device into a socket having a plurality of electrical pins so as to contact at least some and determining at least one parameter by electrical measurement A method for doing so.

  The measurement device may also be part of a measurement sample handling device and may include a handling unit.

  The method can be advantageously applied even if the hand shakes by the patient or the elderly user. A liquid sample, such as a blood sample or another body fluid, is placed on the measurement surface prior to insertion of the measurement device into the socket. Therefore, when handling a liquid sample, there is no power in the measurement device. Furthermore, the measurement of a liquid sample can only be started once the sample has been placed on the measurement surface. In some cases, the measurement surface may be hidden by a closure device to protect the liquid sample and / or prevent evaporation.

  The invention also includes a method for assembly of a measurement sample handling device, the method comprising filling at least one channel in a measurement device having a measurement surface and a plug portion with a solution, at least one of which One channel having at least one channel opening in the measurement surface, inserting the measurement device into the opening of the handling unit so that the measurement surface of the measurement device is accessible, and using the measurement device Closing the channel opening with a protective layer to be removed previously.

  This allows easy, fast and inexpensive fabrication of the measurement sample handling device.

  Insertion of the measuring device into the handling unit may be performed through the first opening. The measuring device may also be inserted, for example, through a third opening on the opposite side of the handling unit. The second opening may also be enlarged or combined with the third opening to allow insertion of the measuring device into the handling unit.

  The step of inserting the measuring device into the opening of the handling unit and the step of closing or sealing the channel opening may be performed immediately after the step of filling at least one channel in order to prevent liquid evaporation.

  The measurement environment may also be kept in a moist or humid environment prior to the closing step, or the step of sealing the channel opening is immediately after the step of removing the measuring device from the moist or humid environment. May be implemented.

  At least one channel in the measuring device may be filled with a solution before use. The solution may be an electrolyte solution (BGE). The solution may also include an electroosmotic flow inhibitor such as polyvinyl alcohol (PVA) or a dynamic coating.

  The term before use is understood in this respect as being before use of the measurement sample handling device by the patient or user. Pre-use also includes prior to shipment to the user or patient.

  The present invention can be better understood by reference to the drawings and detailed description of the preferred embodiments, which are merely exemplary and not limiting of the invention.

FIG. 1 shows a measurement system according to the invention comprising a measurement evaluation device with a socket for a measurement sample handling device comprising a handling unit and a measurement device. 2a-2c show a disposable device according to the invention in exploded view, in an assembled state and in a partial detail view of the disposable device. 3a to 3c are perspective views, side views, and top views, respectively, of the measuring device. FIG. 4 is a schematic diagram showing the measurement device in more detail. FIG. 5 is a detailed view showing a specific embodiment of the two openings of the measuring device. FIG. 6 shows in more detail a plurality of electrical contacts of the measuring device according to the invention. FIG. 7 shows the socket of the measurement evaluation device in more detail. Figures 8a and 8b show a fingertip positioning tool on the handling unit and a plurality of openings and control electrodes in the openings. FIG. 9 shows a cross-sectional view of a handling unit with an inserted measuring device and sealing droplets. FIGS. 10 a to 10 c show a sealing variant and a sealing droplet attached to a measuring device. FIG. 11 is a diagram showing a handling unit having a fixing device for fixing a measuring device in the handling unit. Figures 12a and 12b show the insertion of the measuring device into the handling unit through a third opening in the handling unit. FIG. 13 shows a locking mechanism for closing the handling unit using the closure device.

  In the figures, like reference numerals describe the same or similar.

  FIG. 1 shows a measurement system comprising a combination of a measurement sample handling device or a disposable device 1 including a measurement evaluation apparatus 100 having a socket 110 and a measurement device 10 (shown in FIG. 2 a) attachable to the socket 110. The measurement / evaluation apparatus 100 includes electronic equipment for calculating and evaluating the concentration of seed ions taken from a sample in the measurement sample handling device 1. The measurement evaluation apparatus 100 may include a control device for controlling and examining the measurement and evaluation process. The measurement evaluation apparatus 100 may also include display means such as a display or the like to display the results and settings of the measurement system to the user. The display means is not shown in the figure. The measurement evaluator 100 may also include an interface for connecting the measurement system to a computer or clinical data system (not shown) for data transfer and measurement system control. The measurement evaluation device 110 may also be a personal computer with a socket 110 for receiving the measurement sample handling device 1.

  The measurement sample handling device may be a single use disposable that is only used for a single measurement. However, the disposable device may also be used several times, for example for repeated or parallel measurements. The terms disposable device and measurement sample handling device are used synonymously within this disclosure.

  Figures 2a to 2c show the measurement sample handling device 1 in more detail. In Fig. 2a an exploded view is shown, an assembly view is shown in Fig. 2b and in Fig. 2c a part of the measurement sample handling device 1 that can be attached to the socket 110 is shown in more detail. The measurement sample handling device 1 also includes a handling unit 200. The handling unit 200 has a first opening 210 on the first side 202 defined as the measurement side and a second on the second side 204 of the handling unit 200, as shown in FIGS. 2a and 2c. The opening 220 is provided. The second surface 204 faces toward the socket 110 of the measurement evaluation device 100 when the measurement sample handling device 1 is mounted in the measurement evaluation apparatus 100. The first opening 210 and the second opening 220 may also be located on the bottom surface of the handling unit 200, on the edge of the bottom surface, and on the second surface 204 or any other side of the handling unit 200. Good. The opening may also be enlarged in size to allow insertion of the measuring device 20 into the handling unit 200.

  The first opening 210 and the second opening 220 are interconnected within the handling unit 200 as shown by the dotted lines in FIGS. 2a and 2c.

  The measuring device 10 is inserted into the first opening 210 of the handling unit 200. The measuring device 10 has a measuring surface 20 and a plug part 40. The measuring device 10 may also be inserted through the second opening 220 or the third opening 230, as will be described with respect to FIGS. 9 and 12a, b. The measurement surface 20 is in substantially the same plane as the first side 202 of the handling unit 200 when the measurement device 10 is inserted into the handling unit 200. Thereby, the plug part 40 is accessible from the outside of the measurement sample handling device 1 through the second opening 220 of the handling unit 200. Measurement device 10 is described in further detail below with respect to FIG.

  The measuring device 10 may be made from a different material than the handling unit 200. In particular, the measuring device 10 may be made partially or entirely from a glass material, while the handling unit 200 is made from a plastic material.

  The measuring device 10 may also be formed from a polymer material.

  The measuring device 10 is much smaller than the handling unit 20. Therefore, a millimeter-sized measuring device 10 may be implemented, while the measuring device 10 can be easily handled using the handling unit 200. The size of the handling unit 200 can be adapted to the needs of the user (patient). For example, the handling unit 200 may have dimensions that provide easy handling even with trembling hands. For example, the size of the handling unit 200 may be at least one dimension larger than 1 cm, especially about 4 cm or more. In addition, at least the second side 204 of the handling unit 200 is configured to fit within the socket 110. The sides of the handling unit 200 and other geometric parameters may also be configured to fit within the socket 110.

  The socket 110 and handling unit 200 may be formed in a manner that provides the only possibility that a disposable or measurement sample handling device 1 including the handling unit is inserted into the socket 110. Thereby, erroneous operations by inexperienced or elderly users or patients can be eliminated and measurement errors can be avoided.

  The measuring device 10 can be placed in close proximity to the second side 204 when inserted into the handling unit 200. Therefore, the measurement device is close to the socket 110 when the measurement sample handling device 1 is inserted into the socket 110. The measuring device 10 can be placed inside the handling unit 200 such that the side of the measuring device 10 including the plug 40 is parallel to the second side 204 when inserted into the handling unit 200.

  The handling unit 200 and the measurement surface 20 may be covered by a permeable layer 32 (as clearly seen in FIG. 2a) to provide access to the measurement surface 20 of the measurement device 10. The permeable layer 32 may completely or partially cover the measurement side 202 and the measurement surface 20.

  A sealing body 34 is provided on top of the measurement side 202 to seal the permeable layer 32 or the measurement surface 20 and prevent fluid leakage or evaporation. The sealing layer 34 may be removed by the patient or user prior to use of the measurement device. The permeable layer 32 and the sealing body 34 may be different sizes. One skilled in the art will appreciate that more or fewer layers may be disposed on top of measurement surface 20 or first surface 202.

  The closure device 30 may be used to close the measurement surface 20 before and / or after use. The sealing body 34 and the permeable layer 32 may be attached to the measuring device 10, the closing device 30 or the handling unit 200.

  The handling device 200 and the closure device 30 may be made from the same material, for example a plastic material. The handling device 200 and the closure device 30 may also be made in one piece. An integral hinge may be provided to separate the closure device portion from the handling device portion and to allow the closure device to be stacked on top of the handling device to hide or close the measurement surface 20 .

  3a, 3b and 3c show the measuring device 10 in perspective, side and top views, respectively.

  The measuring device 10 has a first opening 25 in the measuring part 15. A microfluidic channel 60 (shown in FIG. 4) is provided in the measurement portion 15 inside the measurement device 10. The first opening 25 provides access to the microfluidic channel 60 from around the measurement surface 20. One skilled in the art will appreciate that multiple openings 25 can be provided and that the microfluidic channel 60 can include a network of different ones of the channels 60 implemented within the measurement device 10. An example for a channel 60 with a first opening 25 that is particularly useful in connection with the present invention can be found in the patent application PCT / EP2006 / 011148. The measuring device 10 may be at least partially formed in a glass material or another material that can be microstructured.

  The first opening 25 may be in the measurement surface 20. The first opening 25 may also be in another side of the measurement portion 15 of the measurement device 10 that is close to the measurement surface 20 to which the liquid sample is applied. In this case, the liquid sample will travel from the measurement surface 20 to the first opening 25.

  The plug portion 40 is arranged on the side of the measurement device 10 that is different from the measurement surface 20 including the first opening 25. Therefore, when inserted into the handling unit 200, the plug portion 40 is accessible only through the second opening 220 of the handling unit 200, while the measurement surface 20 is the first of the handling unit 200. Can only be accessed through the opening 210. The handling unit 200 may therefore be provided with a seal that ensures that the liquid sample applied to the measurement surface 20 in use cannot contact any of the plurality of electrical contacts 50. Therefore, electrical shorts between two or more of the plurality of contacts 50 that would impair measurement or function control of the measuring device 10 can be advantageously eliminated.

  Plug portion 40 and measurement surface 20 may also be disposed on the same side of measurement device 10. However, the plug portion 40 and the measurement surface 20 are separated from each other by the sealing portion of the handling unit 200 when the measurement device 10 is inserted into the handling unit 200. Therefore, liquid on the measurement surface 20 is prevented from contacting the electrical contacts of the plug portion 40.

  FIG. 4 shows in more detail a schematic diagram of the measuring device 10 in the diagram of FIG. 3b. Microfluidic channel 60 is disposed between two microfluidic reservoirs 61 and 62. The microfluidic channel 60 further has a first opening 25 in the measurement surface 20. The first opening 25 may be connected to the microfluidic channel 60 via the sample channel 26.

  In addition, the electrode 65 may be integrated within the measurement device 10. Electrode 65 may be configured as electrophoretic electrodes 65b and 65c for separating charged species in the sample inside microfluidic channel 60. The electrophoresis electrode 65b may be integrated in each of the reservoirs 61, 62, and 64. The reservoirs 61, 62, 64 may be closed so that the microfluidic channel 60 provides only access to the reservoirs 61, 62, 64. In this way, the liquid inside the reservoir is prevented from evaporating and generating gas. The reservoirs 61, 62, 64 may be substantially larger in size than the width, height or depth of the microfluidic channel 60.

  Each of the electrodes 65b is in electrical contact with the electrical contacts 50b, 50h, and 50g via an electrical path. Therefore, electrophoresis within the microfluidic channel 60 can be controlled by applying a voltage independently to each of the electrophoresis electrodes 65b by the measurement evaluation device 100 when the measurement device 10 is attached to the socket 110. The opening electrode 65c may be integrated at the first opening 25 and connected to the electrical contact 50i. Open electrode 65c may also serve as an electrophoresis electrode or as a control electrode, as will be described later.

  The electrode 65 may also be provided as a conductivity electrode 65a for measuring the conductivity in the section of the microfluidic channel 60 for determining the charge concentration in this section of the microfluidic channel 60. Conductivity electrode 65a is connected to and directed to electrical contacts 50a and 50d (as shown in FIG. 4) and is therefore controlled by measurement evaluation device 100 when measurement device 10 is attached to socket 110.

  The electrophoresis electrode 65b in the capillary electrophoresis system may be based on a material capable of adsorbing hydrogen atoms, such as palladium or platinum, due to its inherent properties. The adsorption makes it possible to prevent the generation of, for example, hydrogen gas near the electrophoretic electrode 65b used as the cathode.

  The use of palladium or platinum as material is particularly useful for electrophoretic electrode 65b used as a cathode, but others of electrode 65 may also be made from the same material.

  The electrophoretic electrode 65b and / or the aperture electrode 65c used as the anode may also be made from different materials to prevent oxygen gas generation. For example, the electrophoretic electrode 65b used as the anode may be a silver / silver chloride electrode or made from copper. In this case, chloride and pure silver or copper ions will be formed instead of oxygen.

  Palladium, platinum, nickel, silver / silver chloride and / or copper and further materials may also be mixed within one or more electrodes 65 to combine the advantages of the respective materials.

  The one or more electrodes 65, 65a, 65b, 65c may be provided with an adhesive layer made of an inert metal such as tantalum or chromium.

  Measurement device 10 may further include electrical components such as temperature sensors, pH sensors and others that may be in electrical contact with and controlled by the remaining electrical contacts 50c, 50c and 50f. It will be apparent to those skilled in the art that the number of electrical contacts 50, 50a to 50i is purely exemplary and that more or fewer electrical contacts can be provided within the scope of the present invention.

  It is an advantage of the present invention that the measuring device 10 can include only passive electrical components such as wires, conductors and electrodes. Active components such as transistors, diodes, flip-flops or other similar active electronic components are not necessary. The measurement device 10 may be electronically controlled by the measurement evaluation device 100. However, the sensor may be integrated into the measurement device 10 that may include a semiconductor element that may also be an active semiconductor element in some cases.

FIG. 5 shows a detailed view of a particular embodiment of the first opening 25 of the measurement device 20 connected to the microfluidic channel 60 by the sample channel 26. In addition, the second opening 27 may be provided, for example, to prevent evaporation of the fluid. The second opening 27 is fluidly connected to the sample channel 26 and the first opening 25. The second opening 27 may be substantially larger in size than the first opening 25. The difference in size results in different contact angles θ ₁ and θ ₂ in the first opening 25 and the second opening 27, respectively, when liquid is filled into the microfluidic system and the sample channel 26. The difference between the contact angles θ ₁ and θ ₂ results in a pressure difference in the first opening 25 and the second opening 27 and when liquid is allowed to evaporate from the first opening 25 and the second opening 27. In the first opening 25, the liquid level will remain essentially the same while the liquid level in the second opening will be reduced due to evaporation.

  One skilled in the art will appreciate that additional openings of different or the same size can be added to modify the evaporation behavior at the first opening 25.

  FIG. 6 shows the plurality of electrical contacts 50 in more detail. Each of the plurality of electrical contacts 50 may be disposed within a hole 42 formed in the plug portion 40 of the measurement device 10. For example, the electrical contact may be provided at the bottom of the hole 42. As shown, each of the plurality of electrical contacts 50 will be positioned within an individual hole 42. In some cases, two or more of the plurality of electrical contacts 50 may also be placed together within a single hole of hole 42. In some cases, the hole 42 may be provided without any contact if the measurement device 10 provides only certain functionality. For example, the electrical contacts 50d, 50e and 50f shown in FIG. 4 may be omitted if no further electrical components are used. However, the plug portion provides a corresponding hole 42 that provides space for the corresponding pin of the socket 110.

  The hole 42 may be circular and cylindrical or conical, or may have any other shape known to those skilled in the art. The conical shape may be used to align or guide the pins of the socket 110 toward each of the plurality of contacts 50. Other shapes of holes 42 may also be implemented within the scope of the present invention.

  Furthermore, the layout or arrangement of the electrical contacts may be varied and is in no way limited to the line-arrangement shown in the figure. It is a feature of the present invention that all electrical contacts of the measurement sample handling device 1 are disposed within the measurement device 10 and that the handling unit 200 does not include any electrical components such as contacts, wiring or the like. is there.

  FIG. 7 shows the socket 110 of the measurement evaluation device 100 in more detail. The socket 110 may be provided in the sidewall of the measurement evaluation device 100, as shown in FIG. 1, or may be provided in a separate socket container that is electrically connectable to the measurement evaluation device 100. .

  The socket 110 includes a plurality of contacts of the measurement device 10 such that at least a portion of the plurality of pins 120 is in electrical contact with at least one of the plurality of contacts 50 when the measurement sample handling device 1 is inserted into the socket 110. A plurality of pins 120 arranged in a pattern corresponding to 50 are included. The number of pins 120 may be below, equal to or above the number of contacts 50 of the measuring device 10. Therefore, the same socket 110 and consequently the same measurement evaluation device 100 may be used for a plurality of different measurement devices 10. The measuring device 10 may be integrated into the measuring device 10 for additional sensors, such as temperature, pH sensors or the like, or for different numbers of electrodes 65 for different applications of the measuring device 10. The number of electrical contacts 50 may be different. The number of electrical contacts 50 may vary, but the number and shape of the holes 42 in the plug portion 40 may vary depending on whether the measurement sample handling or measurement sample handling device 1 with the measurement device 10 is inserted into the socket 110. The number and shape of the pins 120 in the socket 110 may be adapted to provide correct contact and positioning for each of the pins 120.

  The plurality of pins 120 are electrical spring contacts to ensure that the plurality of pins 120 come into contact with corresponding ones of the plurality of electrical contacts 50 when the measuring device 100 is inserted into the socket 110. May be made with. The spring contact may be retracted when the measuring device 10 is inserted into the socket 110 and the electrical contact 50 is pressed against the pin 120, thus preventing damage to the measuring device 10.

  The plurality of pins 120 may be disposed inside the socket 110 as shown in FIG. Therefore, when the measurement sample handling device 1 or simply the measurement device 10 is introduced into the socket 110, the measurement device 10 is positioned entirely or partially within the socket 110. In this case, while keeping the measuring device 10 small and thus inexpensive, no modification to the sample on the measuring surface is possible after the measurement has been started and the electrical contacts are not in the handling unit 200. It is unnecessary.

  In addition, direct electrical connection with a patient or other user is not possible. Thus, the measuring device can be safely used by a patient or other user without specific training or attention. This is important because high voltages such as in the range of 1000 volts may be used during sample measurements.

  The measurement evaluation device 100 may start the measurement only when the measurement sample handling device 1 having the measurement device 10 is correctly inserted into the socket 110. For example, the measurement may be initiated only if the required contacts 50a through 50i are actually in contact with the corresponding pins.

  The actual measurement may be initiated only after a successful control measurement is performed to ensure correct operation of the measuring device 10. The control measurement may be, for example, measuring the sodium concentration in the liquid sample 5. Sodium concentration may be measured and evaluated substantially in parallel with the actual measurement of lithium concentration. For successful control measurements, the sodium concentration must be in a range corresponding to that normally found in blood. If different sodium concentrations are measured, it cannot be guaranteed that something goes wrong in the measurement and that the measured lithium concentration is correct. That measurement will therefore be ignored.

  Additional and initial control can be implemented, for example, by measuring the conductivity or temperature of the background electrolyte solution (BGE), for example to check the correctness of the sodium concentration.

  FIG. 8a shows a specific embodiment of the present invention with the fingertip positioning tool integrated within the handling unit 200 described in detail above with respect to FIGS. 2a to 2c. The rim 212 is provided on one or more sides of the first opening 210 in the measurement side 202 of the handling unit 200. The rim 212 has a shape and height that allows a user (patient) using the measurement sample handling device 1 to easily feel and / or see it when placing a finger on top of the measurement side 202. The rim 212 may be arranged along the first opening 210 around the position where the opening 25 is placed when the measuring device 10 is inserted into the handling unit 200. The rim 212 can also serve as a positioning tool for placing a liquid or blood sample on the measurement surface 20 over the opening 25 because it can be felt by the user's fingertips or easily seen by the eye. This is particularly useful because the aperture 25 itself is too small to be seen by the user (patient).

  A cavity or groove in the measurement surface 20 may also be used as a positioning tool. The cavity or groove has the further advantage that the cavity or groove can act as a collector for the sample liquid and prevent the sample liquid from leaking or spreading over the measurement device.

  As shown in FIG. 4 or FIG. 8b, the aperture electrode 56c present in the first aperture 25 may be used to detect the presence of sample liquid on or around the first aperture 25. For example, the aperture electrode 65c can be at a position or height within the positioning cavity or groove. Therefore, by checking for the presence of the sample liquid 5 and even the presence of a certain amount of sample liquid, it can be ascertained that the required amount of sample liquid has been added, for reliable measurements. Indispensable for.

  FIG. 8 b shows an example of how additional electrodes can be placed in the first opening 25. In addition to the aperture electrode 65c, at least one control electrode 65d, 65e, and 65f can be used. At least one control electrode 65d, 65e, 65f can be positioned proximate to the first opening 25 to measure additional parameters such as the conductivity of the liquid sample. For example, the conductivity of the liquid sample can be measured between the control electrode 65d and the control electrode 65e. The electrode 65f can be made from different materials or provided with a coating to measure different parameters of the liquid sample.

  The channel electrode 65g may be provided in the vicinity of the first opening 25. The channel electrode 65g is in contact with the solution inside the sample channel 26 when the sample channel is filled with the electrolyte solution. In the unlikely event of evaporation of the electrolyte solution, the level of the electrolyte solution will sink below the channel electrode 65g, which can be easily detected by conductivity measurement.

  The channel electrode 65g as well as the opening electrode 65c and the control electrodes 65d, 65e, and 65f can therefore be used for initial control measurements such as, for example, initial conductivity or display for evaporation and / or bubble detection.

  The opening electrode 65c or the control electrode 65d, 65e or 65f or the channel electrode 65g may also be used as an electrophoresis electrode for capillary electrophoresis inside the sample channel 26, for example.

  FIG. 9 shows a cross-sectional view of the handling unit 200 with the measuring device 10 inserted. Prior to use, the sealed droplet 29 may be placed at least above the opening 25. The sealed droplet 29 may be made of silicone, PDMS or other material and covers the opening 25 and hence the microfluidic channel 60 to prevent evaporation and contamination. The seal 34 described above with respect to FIG. 2a may be an adhesive foil to cover the measurement surface before use. The sealed droplet 29 may stick to the adhesive foil. The user (patient) may remove the sticky foil and at the same time the sealed droplet 29 attached thereto, thereby providing access to the opening 25.

  FIGS. 10 a to 10 c show different arrangements of the sealing body 34 and the sealing droplet 29 on the first opening 25 of the measuring device 10. As shown in FIG. 10 a, the sealed droplet 29 may be made of silicon material or the like, and the microfluidic network including the microfluidic channel 60 and the sample channel 26 fills with liquid prior to use of the measurement device 10. May be placed over the first opening 25. Thus, the sealed droplet 29 prevents liquid evaporation from the microfluidic network through the first opening 25. A further sealing body 34, for example in the form of a tape or foil, may be applied to the top of the sealing droplet 29. When the patient or user wishes to use the measuring device 10, the sealing body 34 and the sealing droplet 29 are removed before the liquid sample 5 is added to the first opening 25. The sealed droplet 29 may be attached to the sealed body 34 to facilitate its removal.

  The seal 34 may also include a hole 35 that is substantially aligned with the top of the first opening 25 when the seal 34 is placed on the measurement surface 20 of the measurement device 10 as shown in FIG. 10b. Good. In this case, the sealed droplet 29 may spread through the hole 35 in the sealed body 34 for secure attachment. Therefore, the sealed droplet 29 is removed from the first opening 25 when the user or patient removes the seal 34 prior to use of the measurement device 10.

  The seal 34 may also be attached directly to the measurement surface 20 of the measurement device 10. The sealing body 34 may therefore directly seal the first opening 25. The seal may be a tape or foil made of or covered by silicone or other suitable material.

  Seal 34 and finally seal droplet 29 may also be attached to closure device 30. In this case, the sealing body is removed when the closure device 30 is opened prior to use of the measuring device 10. The seal may also be applied when closing the closure device 30 after placing the liquid sample 5 on the measurement surface 20 to prevent contamination and evaporation of the liquid sample 5.

  One skilled in the art will appreciate that the seal 34 described above with respect to the first opening 25 can also be applied to a further opening in the measurement device 10, such as the second opening 27 described and illustrated with respect to FIG. Will.

  FIG. 11 shows the handling unit 200 of the present invention with a securing device 214 for securing the measuring device 10 within the handling unit 200. The measuring device 10 may be inserted into the handling unit 200 through the first opening 210 as shown in FIG. 2a. A fixation device 214 in the form of a rim can be provided at the opening 210. Thus, the width of the opening 210 in at least one direction may be somewhat less than or equal to the corresponding size of the measurement device 10. The fixation device 214 may act as a fixation or snap-on mechanism for the measurement device 10 in the handling unit 200.

  FIGS. 12 a and 12 b show the insertion of the measuring device 10 into the handling unit 200 through a third opening 230 provided in the handling unit 200. A third opening may be provided on the opposite side of the first side 202 of the handling unit 200. Therefore, the first opening 210 in the handling unit 200 can be smaller in size and can essentially provide only access to the first opening 25 on the measurement surface 20. In this way, the contact seal for preventing the sample liquid from contacting the plug portion 40 can be substantially increased in size. Furthermore, the positioning of the measurement surface 20 and the first opening 25 can be performed more accurately.

  The third opening 230 may also be combined with the second opening 220 to form one enlarged opening for insertion of the plug portion 40 and measurement device 10.

  The securing device 234, which may be a snap-on mechanism, may be handled by the handling unit 200 and / or measurement to ensure the securing and correct positioning of the measuring device 10 within the handling unit 200, as shown in FIG. 12b. Provided at device 10. The seal 34, the permeable layer 32 or other means provided in the handling unit 200 is inserted so as to ensure that the measuring device 10 closes the first opening 25 to prevent contamination and evaporation. Can provide reaction force for when.

  FIG. 13 shows a locking mechanism for closing the handling unit 200 using the closure device 30. The closure device 30 can be provided with a hook 38 for the closure device 30 to cover and protect the measurement surface 20 and the opening 25, in particular after a liquid sample has been placed on the opening 25 of the measurement device 10. In addition, when positioned on the first surface 202 of the handling unit 200, it can engage with a corresponding notch 238 in the handling unit 200. The hook 38 and notch 238 may engage each other in a non-removable manner, forming a snap-on fixation device. In this case, after closing the closure device 30, the handling unit 200 cannot be opened again and therefore cannot be used again. This prevents sample contamination and tampering with the measurement results.

  It will be apparent that other snap-on or locking mechanisms can be used with the present invention. For example, the locking mechanism may be provided as a mechanism that can be opened and closed several times to allow multiple access to the measurement surface. Such mechanisms are generally known and widely used.

  The invention has been described with reference to several embodiments. However, it will be apparent to those skilled in the art that the present invention is not limited thereto. On the contrary, the scope of the invention should be construed in conjunction with the following claims.

The measuring device 10 has a first channel opening 25 in the measuring part 15. A microfluidic channel 60 (shown in FIG. 4) is provided in the measurement portion 15 inside the measurement device 10. The first channel opening 25 provides access to the microfluidic channel 60 from around the measurement surface 20. One skilled in the art will appreciate that multiple openings 25 can be provided and that the microfluidic channel 60 can include a network of different ones of the channels 60 implemented within the measurement device 10. An example for a channel 60 with a first channel opening 25 that is particularly useful in connection with the present invention can be found in the patent application PCT / EP2006 / 011148. The measuring device 10 may be at least partially formed in a glass material or another material that can be microstructured.

The first channel opening 25 may be in the measurement surface 20. The first channel opening 25 may also be in another side of the measurement portion 15 of the measurement device 10 that is close to the measurement surface 20 to which the liquid sample is applied. In this case, the liquid sample will travel from the measurement surface 20 to the first channel opening 25.

The plug part 40 is arranged on the side of the measurement device 10, which is different from the measurement surface 20 including the first channel opening 25. Therefore, when inserted into the handling unit 200, the plug portion 40 is accessible only through the second opening 220 of the handling unit 200, while the measurement surface 20 is the first of the handling unit 200. Can only be accessed through the opening 210. The handling unit 200 may therefore be provided with a seal that ensures that the liquid sample applied to the measurement surface 20 in use cannot contact any of the plurality of electrical contacts 50. Therefore, electrical shorts between two or more of the plurality of contacts 50 that would impair measurement or function control of the measuring device 10 can be advantageously eliminated.

FIG. 4 shows in more detail a schematic diagram of the measuring device 10 in the diagram of FIG. 3b. Microfluidic channel 60 is disposed between two microfluidic reservoirs 61 and 62. The microfluidic channel 60 further has a first channel opening 25 in the measurement surface 20. The first channel opening 25 may be connected to the microfluidic channel 60 via the sample channel 26.

Each of the electrodes 65b is in electrical contact with the electrical contacts 50b, 50h, and 50g via an electrical path. Therefore, electrophoresis within the microfluidic channel 60 can be controlled by applying a voltage independently to each of the electrophoresis electrodes 65b by the measurement evaluation device 100 when the measurement device 10 is attached to the socket 110. The opening electrode 65c may be integrated at the first channel opening 25 and connected to the electrical contact 50i. Open electrode 65c may also serve as an electrophoresis electrode or as a control electrode, as will be described later.

FIG. 5 shows a detailed view of a particular embodiment of the first channel opening 25 of the measurement device 20 connected to the microfluidic channel 60 by the sample channel 26. In addition, a second channel opening 25 may be provided, for example to prevent fluid evaporation. The second channel opening 25 is fluidly connected to the sample channel 26 and the first channel opening 25. The second channel opening 25 may be substantially larger in size than the first channel opening 25. The difference in size results in different contact angles θ ₁ and θ ₂ at the first channel opening 25 and the second channel opening 25 , respectively, when liquid is filled into the microfluidic system and the sample channel 26. The difference in the contact angle theta ₁ and theta ₂ is to bring the pressure difference between the first channel opening 25 and a second channel opening 25, the liquid evaporates from the first channel opening 25 and a second channel opening 25 when is allowed, the in the first channel opening 25 while allowing stay the level of the liquid at essentially the same level, it will reduce the liquid level in the second channel opening 25 due to evaporation.

One skilled in the art will appreciate that additional apertures of different or the same size can be added to modify the evaporation behavior at the first channel aperture 25.

As shown in FIG. 4 or FIG. 8b, the opening electrode 56c present in the first channel opening 25 may be used to detect the presence of on or around the sample liquid in the first channel opening 25 . For example, the aperture electrode 65c can be at a position or height within the positioning cavity or groove. Therefore, by checking for the presence of the sample liquid 5 and even the presence of a certain amount of sample liquid, it can be ascertained that the required amount of sample liquid has been added, for reliable measurements. Indispensable for.

FIG. 8 b shows an example of how additional electrodes can be placed in the first channel opening 25. In addition to the aperture electrode 65c, at least one control electrode 65d, 65e, and 65f can be used. At least one control electrode 65d, 65e, 65f can be positioned proximate to the first channel opening 25 to measure additional parameters such as the conductivity of the liquid sample. For example, the conductivity of the liquid sample can be measured between the control electrode 65d and the control electrode 65e. The electrode 65f can be made from different materials or provided with a coating to measure different parameters of the liquid sample.

The channel electrode 65g may be provided proximate to the first channel opening 25. The channel electrode 65g is in contact with the solution inside the sample channel 26 when the sample channel is filled with the electrolyte solution. In the unlikely event of evaporation of the electrolyte solution, the level of the electrolyte solution will sink below the channel electrode 65g, which can be easily detected by conductivity measurement.

FIGS. 10 a to 10 c show different arrangements of the sealing body 34 and the sealing droplet 29 on the first channel opening 25 of the measuring device 10. As shown in FIG. 10 a, the sealed droplet 29 may be made of silicon material or the like, and the microfluidic network including the microfluidic channel 60 and the sample channel 26 fills with liquid prior to use of the measurement device 10. May be placed over the first channel opening 25. Thus, the sealed droplet 29 prevents evaporation of liquid from the microfluidic network through the first channel opening 25. A further sealing body 34, for example in the form of a tape or foil, may be applied to the top of the sealing droplet 29. When the patient or user wishes to use the measuring device 10, the sealing body 34 and the sealing droplet 29 are removed before the liquid sample 5 is added to the first channel opening 25. The sealed droplet 29 may be attached to the sealed body 34 to facilitate its removal.

The seal 34 also includes a hole 35 that is substantially aligned with the top of the first channel opening 25 when the seal 34 is placed on the measurement surface 20 of the measurement device 10 as shown in FIG. 10b. But you can. In this case, the sealed droplet 29 may spread through the hole 35 in the sealed body 34 for secure attachment. Therefore, the sealed droplet 29 is removed from the first channel opening 25 when the user or patient removes the seal 34 prior to use of the measurement device 10.

The seal 34 may also be attached directly to the measurement surface 20 of the measurement device 10. The sealing body 34 may therefore directly seal the first channel opening 25. The seal may be a tape or foil made of or covered by silicone or other suitable material.

One skilled in the art will recognize that the seal 34 described above with respect to the first channel opening 25 can also be applied to a further opening in the measurement device 10, such as the second channel opening 25 described and illustrated with respect to FIG. You will understand.

FIGS. 12 a and 12 b show the insertion of the measuring device 10 into the handling unit 200 through a third opening 230 provided in the handling unit 200. A third opening may be provided on the opposite side of the first side 202 of the handling unit 200. Therefore, the first opening 210 in the handling unit 200 can be smaller in size and can provide essentially only access to the first channel opening 25 on the measurement surface 20. In this way, the contact seal for preventing the sample liquid from contacting the plug portion 40 can be substantially increased in size. Furthermore, the positioning of the measurement surface 20 and the first channel opening 25 can be performed more accurately.

The securing device 234, which may be a snap-on mechanism, may be handled by the handling unit 200 and / or measurement to ensure the securing and correct positioning of the measuring device 10 within the handling unit 200, as shown in FIG. 12b. Provided at device 10. The seal 34, the permeable layer 32 or other means provided in the handling unit 200 is inserted so as to ensure that the measuring device 10 closes the first channel opening 25 to prevent contamination and evaporation. Can provide a reaction force for when

Claims (73)

A measuring device (10) for taking a liquid sample,
A measuring part (15) having a measuring surface (20) for contacting the liquid sample in use;
A measuring device (10) comprising a plug portion (40) having a plurality of electrical contacts (50) and attachable to a socket (110) of a measurement evaluation device (100).   The measuring device (10) according to claim 1, wherein the plug portion (40) is placed on a different side of the measuring device (10) with respect to the measuring surface (30).   The measuring device (10) according to claim 1 or 2, wherein the measuring device (10) is made at least in part from a glass material.   Measurement according to any of the preceding claims, wherein the measurement portion (15) comprises at least one channel opening (25) providing access to at least one channel (60) in the measurement device (10). Device (10).   The measuring device (10) according to claim 4, wherein the at least one channel (60) is filled with a solution before use.   The measuring device (10) according to claim 5, wherein the solution comprises an electroosmotic flow suppressing substance.   The measuring device (10) according to any of claims 4 to 6, wherein the at least one opening (25) is covered by a partially permeable layer (32).   The measuring device (10) according to any of claims 4 to 7, further comprising an evaporative seal (29, 34) for sealing the at least one opening (25).   The measurement device (10) according to any of claims 4 to 8, further comprising an electrode (65) disposed along the at least one channel (60).   The measuring device (10) according to claim 9, wherein the electrode comprises a gas generation preventing material.   11. A measuring device (10) according to claim 10, wherein the gas generation preventing material of the electrode is selected from the group of nickel, palladium, platinum, silver / silver chloride, copper or mixtures thereof.   The measuring device (10) according to any of claims 9 or 11, wherein at least one of the electrodes (65) comprises an adhesive layer.   The measuring device (10) according to any of claims 9 to 12, wherein the electrode (65) comprises a conductivity electrode (65a).   The measuring device (10) according to any of claims 9 to 13, wherein the electrode (65) comprises an electrophoretic electrode (65b).   15. Measuring device (1) according to any of claims 9 to 14, wherein the electrode (65) comprises at least one open electrode (65c) for measuring the presence of a sample liquid in the first opening (25). 10).   16. The measuring device (10) according to claim 15, wherein the at least one open electrode (65c) is adapted for measuring at least one parameter of the sample liquid (25).   17. Measuring device (10) according to any of claims 9 to 16, wherein the electrode (65) comprises at least one control electrode (65d) for measuring at least one parameter in the supply channel (26). .   The measurement device (10) according to any of claims 9 to 17, wherein each of the electrodes (65) is in electrical contact with at least one of the plurality of electrical contacts (50).   19. Measurement device (10) according to any of claims 4 to 18, further comprising at least one closed reservoir (61, 62) fluidly connected to the at least one channel (60).   The measurement device (10) according to claim 19, wherein the at least one closed reservoir (61, 62) is substantially larger in dimension than the width or depth of the at least one channel (60).   21. A measuring device (10) according to any of claims 4 to 20, further comprising an evaporation preventing means (27).   The measuring device (10) according to claim 21, wherein the means for preventing evaporation comprises a second opening (27) fluidly connected to the first opening (25).   The measuring device (10) according to claim 22, wherein the second opening (27) is substantially larger in size than the first opening (25).   24. A measuring device (10) according to any of claims 4 to 23, wherein the at least one channel (60) is formed of a glass material.   A measuring device (10) according to any preceding claim, wherein the measuring part (15) and the plug part (40) are made at least partly from the same material.   The measuring device (10) according to any of the preceding claims, wherein the measuring part (15) and the plug part (40) are realized as one piece.   A measurement device (10) according to any preceding claim, wherein at least one of the plurality of electrical contacts (50) is positioned in at least one hole (42) in the plug portion (40).   The measuring device (10) according to any of the preceding claims, wherein the liquid sample (5) is a body fluid sample.   The measuring device (10) according to any of the preceding claims, wherein the liquid sample (5) is a blood sample.   The measurement device (10) according to any of the preceding claims, wherein the measurement device is configured to measure lithium in the liquid sample (5).   The measurement device (10) according to any of the preceding claims, further comprising a temperature sensor (80).   A measurement device (10) according to any of the preceding claims, comprising an inactive electrical element.   The measurement device (10) according to any of the preceding claims, further comprising an electrical contact seal for sealing the measurement surface (20) from the at least one of the plurality of electrical contacts (50).   34. A measuring device (10) according to claim 33, wherein the electrical contact seal is provided by a handling unit (200).   A measurement sample handling device (1) comprising a measurement device (10) according to any of claims 1 to 34 and a handling unit (200).   The handling unit (200) has a first opening (210) for the measurement surface (20) and at least a second opening (220) for the plurality of electrical contacts (50). The measurement sample handling device (1) according to 35.   37. A measurement sample handling device (1) according to claim 36, wherein the handling unit (200) is substantially larger in size than the measurement device (10).   38. A measurement sample handling device (1) according to claim 36 or 37, wherein the measurement part (40) of the measurement device (10) is placed inside the handling unit (200).   39. A measurement sample handling device (1) according to any of claims 36 to 38, wherein the handling unit comprises an evaporative seal (29, 34).   40. A measurement sample handling device (1) according to any of claims 36 to 39, further comprising a third opening (230) for insertion of the measurement device (10) into the handling unit (200). ).   41. A measurement sample handling device (42) according to any of claims 36 to 40, further comprising a measurement device fixing mechanism (214, 234) for fixing the measurement device (10) in the handling unit (200). 1).   The measurement sample handling device (1) according to claim 41, wherein the measurement device fixing mechanism (214, 234) is a one-time fixing mechanism.   43. A measurement sample handling device (1) according to claim 41 or 42, wherein the measurement device securing mechanism (214, 234) is a snap-on mechanism.   44. A measurement sample handling device (1) according to any of claims 36 to 43, wherein the measurement surface (20) is substantially concealable by a closure device (30).   45. A measurement sample handling device according to claim 44, further comprising a seal (34) between the closure device (30) and the measurement surface (20) for substantially sealing the measurement surface (20) from the surroundings. Device (1).   46. A measurement sample handling device (1) according to claim 44 or 45, wherein the closure device (30) is substantially larger in size than the measurement surface (20).   47. A measurement sample handling device (1) according to any of claims 44 to 46, wherein the handling unit (200) and the closure device (30) are realized as one piece.   48. A measurement sample handling device (1) according to any of claims 44 to 47, further comprising a closure device securing mechanism (38, 238) for concealing the measurement surface (20) with the closure device (30).   49. A measurement sample handling device (1) according to claim 48, wherein the closure device fixation mechanism (38, 238) is a one-time fixation mechanism.   50. A measurement sample handling device (1) according to claim 48 or 49, wherein the closure device securing mechanism (38, 238) is a snap-on mechanism. A socket (110) connectable with a measuring and evaluating device (100) for evaluating at least one parameter of a liquid sample (5),
A measuring device (10) according to any of claims 1 to 33 or any of claims 34 to 48, comprising a plurality of electrical pins (120), said plurality of pins (120) being attachable to said socket. A socket (100) arranged to make electrical contact with a plurality of electrical contacts of a measurement sample handling device (1) according to the above.   52. The socket (110) of claim 51, wherein the socket (110) is disposed on one side of the measurement evaluation device (100).   53. The socket (110) of claim 51 or 52, wherein the electrical pin comprises a spring contact.   54. A measurement evaluation device (100) for evaluating at least one parameter of a liquid sample (5), said measurement evaluation device (100) comprising a socket according to any of claims 51 to 53.   55. The measurement and evaluation device of claim 54, wherein the at least one parameter includes a concentration of lithium ions.   A combination of a measurement evaluation device (100) according to any of claims 54 to 55 and a measurement device (10) according to any of claims 1 to 34.   A combination of a measurement evaluation device (100) according to any of claims 54 to 55 and a measurement sample handling device (1) according to any of claims 35 to 50. In a method for evaluating at least one parameter of a liquid sample (5),
Placing the liquid sample (5) on a measurement surface (20) of a measurement device (10), the measurement device (10) having a plurality of electrical contacts (50);
The measuring device (110) in a socket (110) having the plurality of electrical pins (120) such that at least some of the plurality of electrical pins (120) are in contact with at least some of the plurality of electrical contacts (50). 10) inserting,
Determining the at least one parameter by electrical measurement.   59. Method according to claim 58, wherein the measuring device (10) is part of a measuring sample handling device (1) according to any of claims 34 to 49.   60. A method according to claim 58 or 59, further comprising the step of partially removing the seal (34) covering the first opening (25).   61. A method according to any of claims 58 to 60, further comprising opening the closure device (30).   62. Placing the liquid sample (5) on the measurement surface (20) comprises identifying the position of the first opening (25) with a positioning tool (212). The method described in 1.   63. A method according to any of claims 58 to 62, wherein the liquid sample (5) is a sample of body fluid.   64. A method according to any of claims 58 to 63, wherein the liquid sample (5) is a blood sample.   65. A method according to any of claims 58 to 64, wherein the at least one parameter is a concentration of lithium ions. In a method for assembling a measurement sample handling device (1) for taking a liquid sample (5),
Filling at least one channel (60) in a measuring device (10) having a measuring surface (10) and a plug portion (40) with a solution, said at least one channel (60) being said measuring surface ( 20) having at least one channel opening (25) within;
Inserting the measurement device (10) into the opening (210, 230) of the handling unit (200) so that the measurement surface (20) of the measurement device (10) is accessible;
Closing the channel opening (25) with a protective layer (29, 34, 30) to be removed before use of the measuring device (10).   Inserting the measuring device (10) into the opening of the handling unit and closing the channel opening (25) is performed immediately after filling the at least one channel (60). Item 67. The method according to Item 66.   The step of inserting the measuring device (10) into the opening of the handling unit and closing the channel opening (25) is performed immediately after the step of removing the measuring device from a moist environment. Item 67. The method according to Item 66.   69. A method according to any of claims 66 to 68, further comprising applying an evaporative seal (29, 34).   70. A method according to any of claims 66 to 69, further comprising applying an electrical contact seal.   71. A method according to any of claims 66 to 70, further comprising providing a closure device (30) for concealing the channel opening (25) after adding the sample (5).   72. A method according to any of claims 66 to 71, wherein the protective layer is a seal (28, 34) for sealing the first opening (25).   73. A method according to any of claims 66 to 72, wherein the protective layer is a closure device (30) for repeatedly concealing the opening.

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