JP2013113633A - Strip - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new strip for providing improved sensitivity for biochemical testing that uses an immunochromatography method or the like.SOLUTION: A hold part for holding molecules that support reaction in a biochemical testing, such as an immunochromatography method, is provided with: (i) a plurality of fine projections composed of resin having hydrophilic surfaces and/or (ii) a mesh composed of resin having hydrophilic surfaces. The hold part holds the molecules for supporting reactions in the biochemical testing. The current invention also provides a strip for simplifying affinity purification.

Description

  The present invention relates to a strip used for biochemical tests or purification of target substances. The strip of the present invention comprises (i) a plurality of minute protrusions made of a resin having a hydrophilic surface as a holding part for holding a molecule responsible for a reaction in a biochemical test or a molecule that specifically binds a target substance. And / or (ii) a mesh made of a resin having a hydrophilic surface.

  In order to easily perform biochemical tests using immunochromatography or the like, strips using filter paper or the like are used. In the conventional immunochromatographic strip, the antibody solution that detects the target substance in the sample is applied to the filter paper as a test line, the antibody solution that detects the control substance is applied to the filter paper as the control line, and the sample moves through the strip. In doing so, the target substance or control substance reacts with these antibodies. The amount of antibody retained by applying the antibody solution to the test line or control line depends on the concentration of antibody in the solution and the amount of antibody solution to be applied. Therefore, in order to increase the detection sensitivity, it is necessary to apply more antibody solution. However, when the amount of the antibody solution to be applied is increased, the width of the test line or control line becomes wider and the reaction becomes difficult to see. Therefore, a new strip is sought in order to provide a higher detection sensitivity than in conventional methods using strips that are filter paper.

7-60158

  An object of the present invention is to provide a novel strip capable of providing improved sensitivity for biochemical tests using immunochromatography and the like.

  An object of the present invention is to provide (i) a plurality of minute protrusions made of a resin having a hydrophilic surface and / or (ii) hydrophilicity as a holding part for holding molecules responsible for reactions in biochemical tests such as immunochromatography. This problem has been solved by providing a mesh made of a resin having a conductive surface. The holding part holds molecules responsible for reactions in the biochemical test.

For example, the present invention provides the following.
(Item 1)
A strip including a sample introduction part, a development part, and a holding part, wherein the holding part is a plurality of minute protrusions made of a resin having a hydrophilic surface and / or a mesh made of a resin having a hydrophilic surface And the retainer retains a material for biochemical testing on its surface.
(Item 2)
A strip including a sample introduction part, a development part, and a holding part, wherein the holding part is a plurality of minute protrusions made of a resin having a hydrophilic surface and / or a mesh made of a resin having a hydrophilic surface And the holding part holds a substance that specifically binds to the target substance.
(Item 3)
Item 3. The strip according to Item 1 or 2, wherein the surface of the resin having a hydrophilic surface has absorption in the vicinity of 3500 / cm in the infrared absorption spectrum.
(Item 4)
4. The strip according to any one of items 1 to 3, wherein the surface of the resin having a hydrophilic surface is subjected to a hydrophilic treatment.
(Item 5)
The strip according to item 4, wherein
Here, the conditions of the hydrophilic treatment in the case of revolving the strip are as follows: input power is 0.4 to 3 (KW), treatment time is 1 to 300 (seconds), and degree of vacuum is 0.1 to 50 (Pa ) And
Here, the conditions of the hydrophilic treatment when the substrate is stationary are as follows: input power is 0.4 to 3 (KW), treatment time is 1/50 to 60 (seconds), and vacuum is 0.1 to 50 ( Pa), the strip.
(Item 6)
Item 6. The strip according to any one of Items 1 to 5, wherein the resin is a transparent resin selected from the group consisting of polycarbonate, ABS resin, acrylic resin, and polystyrene.
(Item 7)
The plurality of protrusions have a predetermined height (H), a diameter (D), and a mutual interval, the height (H) of the protrusion is 10 to 5000 μm, the diameter (D) of the protrusion is 10 nm to 500 μm, Item 7. The strip according to any one of Items 1 to 6, wherein an interval between the protrusions is 0.1 μm to 1000 μm.
(Item 8)
2. The strip according to item 1, wherein the biochemical test is a test using an immunochromatography method, and the substance for the biochemical test is an antibody.
(Item 9)
2. The strip according to item 1, wherein the biochemical test is a detection test for a metal atom, and the substance for the biochemical test is a substance that forms a complex with the metal atom.
(Item 10)
A strip according to item 1, comprising a filter paper.
(Item 11)
Item 3. A strip according to item 1 or 2, comprising a microchannel through which a sample moves.
(Item 12)
Item 12. The strip according to Item 11, wherein the surface of the microchannel contains a resin subjected to hydrophilic treatment.
(Item 13)
Item 12. The strip according to item 11, wherein the microchannel has a width of 0.05 to 5 mm.
(Item 14)
Item 12. The strip according to item 11, wherein the microchannel is a plurality of microchannels arranged in parallel.

  The present invention also provides a strip for convenient affinity purification.

  Since the strip of the present invention includes (i) a plurality of minute protrusions made of a resin having a hydrophilic surface and / or (ii) a mesh made of a resin having a hydrophilic surface, Arbitrary substances do not diffuse and are limited to a predetermined position. Moreover, it becomes possible to fix | immobilize the arbitrary substances for a larger amount of biochemical tests in a fixed area compared with the conventional method using a filter paper. As a result, it is possible to prevent a decrease in accuracy or sensitivity due to unclearness of the signal caused by diffusion.

  The fine protrusions and / or meshes of the present invention can adjust the density of any material for biochemical testing per fixed area of the strip by adjusting its height, Therefore, the amount of biochemical reaction per certain area can be adjusted. As a result, the strip of the present invention can increase the amount of biochemical reaction per the same area as compared with the conventional method, and therefore can increase the sensitivity as compared with the conventional method. .

  Furthermore, the strip of the present invention can also be used for simple affinity purification.

FIG. 1 is a cross-sectional view of the strip (11) of the present invention. The strip (11) includes an introduction part (21), a development part (31), and a holding part (41). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 2 is a cross-sectional view of the strip (12) of the present invention. The strip (12) comprises an introduction part (21), a development part (31), a holding part (41) and a further development part (32). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 3 is a cross-sectional view of the strip (13) of the present invention. The strip (13) includes an introduction part (21), a development part (31), a holding part (41), and an absorption part (51). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 4 is a cross-sectional view of the strip (14) of the present invention. The strip (14) comprises an introduction part (21), a development part (31), a holding part (41), a further development part (32), a further holding part (42) and a further development part (33). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 5 is a plan view of the strip (12) of the present invention with a single microchannel. The strip (12) comprises an introduction part (21), a development part (31), a holding part (41) and a further development part (32). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 6 is a plan view of the strip (12) of the present invention comprising a plurality of microchannels. The strip (12) comprises an introduction part (21), a development part (31), a holding part (41) and a further development part (32). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 7 is a cross-sectional view of the strip (15) of the present invention where the sample is developed using gravity. The strip (12) comprises an introduction part (21), a development part (31), a holding part (41) and a further development part (32). The height of the bottom surface of each of these parts becomes lower according to the development direction. The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of arrow A by the development part. FIG. 8 is a cross-sectional view of a holding portion including a plurality of minute protrusions made of a resin having a hydrophilic surface. FIG. 9 is a perspective view of a main part of the holding unit shown in FIG.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. However, it should be understood that these embodiments are examples of the present invention, and the scope of the present invention is not limited to such preferred embodiments. It is. It should be understood that those skilled in the art can easily make modifications, changes and the like within the scope of the present invention with reference to the following preferred embodiments.

(1. Strip of the present invention)
The strip of the present invention includes (i) a plurality of minute protrusions made of a resin having a hydrophilic surface and / or (ii) a holding portion including a mesh made of a resin having a hydrophilic surface (41, 42). .

  In one aspect, the strip (11) of the present invention includes an introduction part (21), a development part (31), and a holding part (41) (FIG. 1). The introduction part (21) and the development part (31) may be different parts of the same member. The sample introduced into the introduction part is developed in the direction of the holding part by the developing part (in the direction of arrow A in FIG. 1) and moves to the holding part. The sample that has moved to the holding unit comes into contact with molecules responsible for the reaction in the biochemical test held by the holding unit. The strip (12) of the present invention may further include a developing part (32) in the direction of developing the sample, adjacent to the holding part (41) (FIG. 2).

  The strip of the present invention may include an absorption part (51) for absorbing the developed liquid phase at the end in the direction of developing the sample. FIG. 3 is a strip (13) further comprising an absorbent part (51) adjacent to the holding part (41) at the end of the strip (11) of FIG. In this invention, you may further provide the absorption part (51) adjacent to the expansion | deployment part (32) in the edge part of the strip (12) of FIG.

  The strip of the present invention may include two or more holding portions. For example, the strip (12) of the present invention shown in FIG. 2 further includes a holding part (42) and a developing part (33) adjacent to the developing part (32) at the end in the direction in which the sample is developed. (14) (FIG. 4). The developing part (32) between the holding part (41) and the holding part (42) may be omitted. In this case, the holding part (41) and the holding part (42) Directly adjacent without. Further, the development portion (33) is not essential and can be omitted in the strip (14). In the strip (14 or 14 lacking 33), an absorption part (51) for absorbing the developed liquid phase may be provided at the end in the direction of developing the sample.

  Similarly, the strip of the present invention may comprise more than two holding parts.

(1. Introduction part)
The sample to be analyzed is introduced into the introduction part. The introduction part may be constituted by the same member as the development part adjacent to the introduction part or may be constituted by a different member. The introduction part and the development part adjacent to the introduction part do not necessarily need to be physically distinguished. Moreover, you may utilize a part of expansion | deployment part as an introduction part.

(2. Development part)
The developing part provided in the strip of the present invention can use any material capable of developing the sample solution. When utilizing capillary action, it is possible to use, for example, filter paper, membranes, and / or microchannels.

(2.1. Filter paper or membrane)
As the filter paper or membrane, materials usable for immunochromatography can be used. For example, examples of the membrane include, but are not limited to, a nitrocellulose membrane and cellulose acetate.

(2.2. Micro flow path of molding resin whose surface is hydrophilic)
When utilizing the capillary phenomenon, it is possible to utilize a micro flow channel of a molding resin whose surface is subjected to hydrophilic treatment. As the resin, polyester, ABS (acrylonitrile / butadiene / styrene copolymer), acrylic resin, PC (polycarbonate), polyamide, PS (polystyrene) and other thermoplastic resins, biodegradable resins, UV curable resins, etc. are used. can do. Usually, a thermoplastic resin is used. In particular, an amorphous resin that is transparent and excellent in moldability is preferable. Particularly preferred resins are transparent resins such as PC, ABS, PMMA (acrylic), and PS.

  Any method can be used for hydrophilic treatment of the resin surface. A typical hydrophilic treatment is a method in which a gas such as atmospheric gas or argon is put in an apparatus, and the gas is activated in a vacuum and applied to the substrate surface to increase hydroxyl groups on the substrate surface. By selecting the gas to be used, the power supply system (RF, DC, etc.) and the degree of vacuum (high vacuum (about 10 −3 Pa or higher) to low vacuum (about 1 to 100 Pa)), the hydrophilic treatment conditions are Can be adjusted. Preferably, the water contact angle on the surface of the substrate is set to 20 ° or less, or 2 to 15 °, or 10 ° or less by hydrophilic treatment. The water contact angle can be measured in accordance with JIS R3257 (static drop method, also simply referred to as θ / 2 method). The hydrophilic treatment conditions may be changed according to the type of resin, such as the degree of vacuum, input power, and treatment time.

  In general, when the distance between the electrode and the substrate to be processed is about 1 to 300 mm and the substrate is revolved and processed, the input power is 0.4 to 3 (KW), the processing time is 1 to 300 (seconds), and the degree of vacuum Is 0.1 to 50 (Pa). Preferably, the input power is 1.6 to 2 (KW), the processing time is 60 to 120 (seconds), and the degree of vacuum is 5 to 20, particularly 10 (Pa). In addition, the substrate can be processed while being stationary, and in this case, the processing time may be set to 1/5 to 1/50 in the case of revolution.

  An appropriate width of the microchannel for utilizing the capillary phenomenon can be appropriately determined by those skilled in the art in consideration of the viscosity of the sample to be used. The width of the microchannel is typically 0.05 mm to 5 mm, preferably 0.1 mm to 5 mm, more preferably 1 mm to 3 mm, and most preferably 1 mm to 2 mm.

  As the micro flow channel of the present invention, for example, a single flow channel can be used as shown in FIG. On the other hand, since the width of the microchannel is limited in order to utilize the capillary phenomenon, in the present invention, as shown in FIG. 6, a plurality of parallel microchannels are produced as necessary. You can also.

(2.3. Deployment by centrifugal force)
It is also possible to deploy using a centrifugal force without using the capillary phenomenon or with the capillary phenomenon. A person skilled in the art can appropriately select the degree and time of the centrifugal force.

(2.4. Deployment by gravity)
It is also possible to deploy using gravity without using the capillary phenomenon or with the capillary phenomenon. For example, as shown in FIG. 7, the sample can be developed by gravity by lowering the bottom surface of each part in accordance with the direction in which the sample is developed. The difference in the height of the bottom surface can be appropriately selected by those skilled in the art in order to obtain a desired development speed. It is also possible to combine the development by gravity with the development by centrifugal force.

(2.5. Development by adding liquid phase)
When using the capillary phenomenon, if the amount of the liquid phase remaining in the introduction portion is small, the speed of development is reduced. Therefore, by limiting the amount of liquid phase (for example, sample solution) to be added to the introduction, it is possible to reduce the speed of development or to temporarily stop development. In such a case, it is possible to resume deployment or increase the deployment speed by adding a liquid phase to the introduction.

(3. Holding part)
The holding part provided in the strip of the present invention is composed of (i) a plurality of minute protrusions made of a resin having a hydrophilic surface and / or (ii) a mesh made of a resin having a hydrophilic surface.

(3.1. Plural minute protrusions made of a resin having a hydrophilic surface)
Resin that can be used for a plurality of minute protrusions made of a resin having a hydrophilic surface, a hydrophilic treatment, and a preferable water contact angle are described in 2.2. It is as described in.

  In a plurality of minute protrusions made of a resin having a hydrophilic surface, the protrusion 2 extends in the vertical direction from the surface of the substrate 1 as shown in FIGS. The height, diameter, and spacing between adjacent protrusions can be set according to the type of resin, the degree of hydrophilicity (contact angle) of the substrate, the purpose of use, and the like. The height (H) is preferably 10 to 5000 μm, the diameter (D) of the protrusions is preferably 10 nm to 500 μm, and the distance between the protrusions is preferably 0.1 μm to 1000 μm.

  In the present invention, the diameter D of the protrusion 2 is an equivalent diameter at an intermediate position of the protrusion. Note that the term equivalent diameter is used because the cross section of the protrusion is not necessarily circular, but may be an ellipse, a polygon, an asymmetric shape, etc. In the present invention, the equivalent diameter is used to encompass all of these. . The aspect ratio (H / D) is preferably 4 or more, and more preferably 8 to 30. However, 100 or less is preferable from the viewpoint of structural strength. The shape of the protrusion is typically a substantially cylindrical shape. By carrying out chemical or biological treatment on the surface of the protrusion, the molecule responsible for the reaction in the biochemical test is retained. Examples of the chemical treatment include, but are not limited to, active esterification with N-hydroxysuccinimide (NHS), activation with bromocyan (CNBr), epoxy activation, and SDS treatment.

(3.2. Mesh made of resin having hydrophilic surface)
Resin that can be used for a mesh made of a resin having a hydrophilic surface, a hydrophilic treatment, and a preferable water contact angle are described in 2.2. It is as described in.

  The mesh size of the mesh can be appropriately determined by those skilled in the art based on the sample to be used and the target biochemical reaction. The mesh size of the mesh is typically 0.1 μm to 1000 μm, preferably 0.5 μm to 500 μm, more preferably 0.5 μm to 10 μm, and most preferably 0.1 μm to 1.0 μm. The thickness of the mesh is typically 0.01 mm to 10 mm, preferably 0.05 mm to 5.0 mm, more preferably 0.1 mm to 5.0 mm, and most preferably 1.0 mm to 3.0 mm.

(3.3. Multiple holding units)
The strip of the present invention may include two or more holding portions (see FIG. 4). For example, one holding unit may be used for detection of a target molecule, and another holding unit may be used as a control. When two or more holding parts are provided, these holding parts are preferably separated by a developing part, but in a specific aspect, two or more holding parts may be adjacent to each other.

(4. Absorbing part)
In order to remove an unnecessary liquid phase or promote capillary action, the end of the strip of the present invention (the end opposite to the introduction part) may be provided with a liquid phase absorption part (see FIG. 3). ). Examples of the absorption part of the present invention include a filter paper or absorption pad that absorbs the liquid phase, a liquid phase reservoir having a bottom surface lower than the bottom surface of the adjacent developing part or holding part, and a liquid phase suction device. It is not limited.

(2. Use of strip of the present invention)
The strip of the present invention can be used for any biochemical test. As will be described later, the strip of the present invention can be used for isolation and purification of a target substance.

(1. Immunochromatography)
The strip of the present invention can be used for immunochromatography. Two types of antibodies against the target molecule are prepared, and the first antibody is immobilized on the holding part (41). The labeled second antibody is mixed with the sample to be analyzed and introduced into the introduction section (21). The sample / antibody mixture in which the developing part (31) is developed moves to the holding part (41). When the target molecule is present in the sample, the target molecule forms a complex with the second antibody, and the complex binds to the immobilized first antibody. As a result, a complex of [immobilized first antibody]-[target molecule]-[labeled second antibody] is formed in the holding part. By detecting the label of the second antibody in the complex, it is possible to detect the presence of the target molecule in the sample. (In this case, it is preferable that the binding between the target substance and the first antibody is not affected by the binding between the target substance and the second antibody.)
If necessary, the strip is provided with a further holding part. A further holding part can be used as a control. For example, by immobilizing an antibody specific to the labeled second antibody on the additional holding part, the mixture containing the sample is appropriately developed, and the label is appropriately applied to the second antibody. Can be confirmed.

  When there are a plurality of target substances, an additional holding part is provided to immobilize an antibody that specifically binds to another target substance.

  Any substance that can elicit an antibody can be an immunochromatographic detection target. Examples of such substances include, but are not limited to, food allergy allergens (eg, but not limited to gluten, ovomucoid, ovalbumin, soy protein, chitin, etc.), heavy metals ( Examples include, but are not limited to, cadmium, cesium, mercury, arsenic), viruses (including but not limited to norovirus, rotavirus, influenza virus), mycoplasma, spirochetes, pathogenic protozoa, bacteria ( Escherichia coli, Salmonella, Pseudomonas aeruginosa, staphylococci, hemolytic streptococci, pneumococci, tuberculosis, and the like), agonists, antagonists, hormones, cytokines, nucleic acids, DNA, RNA, and fragments thereof, As well as combinations of these But are lower but are not limited to these.

(2. Purification)
The strip of the present invention can be used for purification of a target substance.

  A sample containing the target substance is introduced into the introduction part (21) and moved to the holding part (41) via the development part (31). A molecule that specifically binds to the target substance (for example, an antibody or a ligand when the receptor is the target substance, a receptor when the ligand is the target substance, a monomer or a complex when the multimer is formed in the holding part. The target substance in the sample that has moved to the holding part is captured by the holding part because the component of the complex or any binding partner (including but not limited to this) is immobilized. . The captured target substance is released from the holding part by a change in salt concentration and / or a change in pH, or by cleavage of the immobilized molecule. By collecting the released target substance, the target substance can be purified.

(3. Detection)
When using the strip of the present invention for detection, any known detection method can be utilized. For example, when used for immunochromatography as described in (1) above, it is possible to visually detect the formation of a complex in the holding part by labeling the second antibody with a gold colloid.

  In addition to colloidal gold, it is possible to label using a fluorescent substance, biotin, enzyme, or phenylboronic acid.

  Hereinafter, the present invention will be described in detail with reference to examples and the like, but the present invention is not limited thereto.

Example 1
Whether or not the immune reaction can be confirmed with the naked eye on the nanoimprint film of methyl acrylate was observed by the following experiment.

(Preparation of nanoimprint film)
Protrusions having a height (H) of 30 μm, a diameter (D) of 10 μm, and a spacing of 15 μm were formed on the methyl acrylate resin. Next, the resin was stopped, and hydrophilic treatment was performed with an applied power of 2.0 (KW), a processing time of 60 (seconds), and a vacuum of 0.1 (Pa) to produce a methyl acrylate nanoimprint film. . This nanoimprint film corresponds to the holding part of the present invention.

  An anti-CRP mouse antibody bound to a gold colloid with an average particle diameter of 400 nm was dropped onto the nanoimprint film, allowed to spread uniformly, and then dried at room temperature for 15 to 20 minutes. Next, the CRP protein solution which is an antigen of various concentration was dripped.

  As a result, the portion where the antigen was dropped spread concentrically, and a purple ring was formed along the concentric circle, confirming that an antigen-antibody reaction occurred.

  As mentioned above, although this invention has been illustrated using preferable embodiment of this invention, this invention should not be limited and limited to this embodiment. It is understood that the scope of the present invention should be construed only by the claims. It is understood that those skilled in the art can implement an equivalent range based on the description of the present invention and the common general technical knowledge from the description of specific preferred embodiments of the present invention. Patents, patent applications, and documents cited herein should be incorporated by reference in their entirety, as if the contents themselves were specifically described herein. Understood.

  Since the strip of the present invention includes (i) a plurality of minute protrusions made of a resin having a hydrophilic surface and / or (ii) a mesh made of a resin having a hydrophilic surface, Arbitrary substances do not diffuse and are limited to a predetermined position. Moreover, it becomes possible to fix | immobilize the arbitrary substances for a larger amount of biochemical tests in a fixed area compared with the conventional method using a filter paper. As a result, it is possible to prevent a decrease in accuracy or sensitivity due to unclearness of the signal caused by diffusion.

  The fine protrusions and / or meshes of the present invention can adjust the density of any material for biochemical testing per fixed area of the strip by adjusting its height, Therefore, the amount of biochemical reaction per certain area can be adjusted. As a result, the strip of the present invention can increase the amount of biochemical reaction per the same area as compared with the conventional method, and therefore can increase the sensitivity as compared with the conventional method. .

  Furthermore, an apparatus for conveniently performing affinity purification is also provided according to the present invention.

DESCRIPTION OF SYMBOLS 1 Substrate 2 Protrusion 11, 12, 13, 14, 15 Strip 21 Introduction part 31, 32, 33 Deployment part 41, 42 Holding part 51 Absorption part

Claims (14)

  A strip including a sample introduction part, a development part, and a holding part, wherein the holding part is a plurality of minute protrusions made of a resin having a hydrophilic surface and / or a mesh made of a resin having a hydrophilic surface And the retainer retains a material for biochemical testing on its surface.   A strip including a sample introduction part, a development part, and a holding part, wherein the holding part is a plurality of minute protrusions made of a resin having a hydrophilic surface and / or a mesh made of a resin having a hydrophilic surface And the holding part holds a substance that specifically binds to the target substance.   The strip according to claim 1 or 2, wherein the surface of the resin having a hydrophilic surface has absorption in the vicinity of 3500 / cm in an infrared absorption spectrum.   The strip according to any one of claims 1 to 3, wherein a surface of the resin having the hydrophilic surface is subjected to a hydrophilic treatment. The strip according to claim 4,
Here, the conditions of the hydrophilic treatment in the case of revolving the strip are as follows: input power is 0.4 to 3 (KW), treatment time is 1 to 300 (seconds), and degree of vacuum is 0.1 to 50 (Pa ) And
Here, the conditions of the hydrophilic treatment when the substrate is stationary are as follows: input power is 0.4 to 3 (KW), treatment time is 1/50 to 60 (seconds), and vacuum is 0.1 to 50 ( Pa), the strip.   The strip according to any one of claims 1 to 5, wherein the resin is a transparent resin selected from the group consisting of polycarbonate, ABS resin, acrylic resin, and polystyrene.   The plurality of protrusions have a predetermined height (H), a diameter (D), and a mutual interval, the height (H) of the protrusion is 10 to 5000 μm, the diameter (D) of the protrusion is 10 nm to 500 μm, The strip according to any one of claims 1 to 6, wherein an interval between the protrusions is 0.1 µm to 1000 µm.   The strip according to claim 1, wherein the biochemical test is a test using an immunochromatography method, and the substance for the biochemical test is an antibody.   The strip according to claim 1, wherein the biochemical test is a metal atom detection test, and the substance for the biochemical test is a substance that forms a complex with the metal atom.   The strip according to claim 1, comprising a filter paper.   3. A strip according to claim 1 or 2, comprising a microchannel through which the sample moves.   It is a strip of Claim 11, Comprising: The strip containing the resin by which the surface of the said microchannel was hydrophilically processed.   The strip according to claim 11, wherein the width of the microchannel is 0.05 to 5 mm.   The strip according to claim 11, wherein the microchannel is a plurality of microchannels arranged in parallel.

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