JP2013076673A - Measurement apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prevent a measurement result from being influenced by dew condensation even when the dew condensation is generated in a temperature controlling member for controlling a temperature of an analysis chip.SOLUTION: A measurement apparatus using a photodetection method for analyzing a test substance by detecting light generated from a photoresponsive labeled substance combined with a substance to be detected in a sensor part of an analysis chip 10 includes: an aluminum block 70 for controlling the temperature of the analysis chip 10 by bringing the analysis chip 10 into contact with a predetermined measurement position of the upper surface of the aluminum block 70; temperature control means 80 for controlling the temperature of the aluminum block 70; and analysis chip moving means, not illustrated, for moving the analysis chip 10 loaded from the external up to the measurement position while sliding on the upper surface of the aluminum block 70. A slit 71 for collecting waterdrops accumulated when the analysis chip 10 slides on the upper surface of the aluminum block 70 is formed on the upper surface.

Description

  The present invention relates to a measuring apparatus using a photodetection method for analyzing light to be detected by detecting light generated from a photoresponsive labeling substance bound to a substance to be detected in a sensor portion of an analysis chip.

  In bio-measurement and the like, the fluorescence method is widely used as a highly sensitive and easy measurement method. The fluorescence method is qualitative or qualitative by irradiating a sample considered to contain a substance to be detected that is excited by light of a specific wavelength to emit fluorescence and irradiating the excitation light of the specific wavelength and detecting the fluorescence emitted at this time. This is a method for quantitatively confirming the presence of a substance to be detected. In addition, when the substance to be detected is not a fluorescent material, the substance to be detected is labeled with a fluorescent label such as an organic fluorescent dye, and then the fluorescence is detected in the same manner. It is a method to confirm.

  In the above fluorescence method, for the reason that only a specific target substance can be efficiently detected while flowing a sample, the target substance is fixed on the surface of the sensor part of the analysis chip by the following two methods, and then fluorescence detection is performed. The technique to perform is common. One such technique is, for example, when the substance to be detected is an antigen, the antigen is specifically bound to the primary antibody immobilized on the surface of the sensor unit, and then a fluorescent label is attached. A secondary antibody that specifically binds to the antigen, and further binds to the antigen to form a primary antibody-antigen-secondary antibody binding state, and the fluorescence from the fluorescent label attached to the secondary antibody This is a so-called sandwich method for detection. The other is, for example, when the substance to be detected is an antigen, a secondary antibody in which an antigen and a fluorescent label are attached to the primary antibody immobilized on the surface of the sensor unit (unlike the above-described secondary antibody, Is a so-called competition method in which the primary antibody is bound to the primary antibody competitively and the fluorescence from the fluorescent label attached to the competitively bound secondary antibody is detected. .

  In addition, for the reason that the S / N ratio can be improved in fluorescence detection, an evanescent fluorescence method has been proposed in which a fluorescent label indirectly fixed to a sensor unit by the above-described method is excited by evanescent light. In the evanescent fluorescence method, excitation light is incident from the back surface of the sensor unit, and the fluorescent label is excited by evanescent light that oozes out to the surface of the sensor unit, and fluorescence generated from the fluorescent label is detected.

  On the other hand, in order to improve sensitivity in the evanescent fluorescence method, a method using the effect of electric field enhancement by plasmon resonance has been proposed in Patent Document 1, Non-Patent Document 1, and the like. In the surface plasmon enhanced fluorescence method, in order to generate plasmon resonance, a metal layer is provided in the sensor unit, surface plasmon is generated in the metal layer, and the S / N ratio is increased by increasing the fluorescence signal by the electric field enhancing action. It is to improve.

  Further, in the evanescent fluorescence method, as in the surface plasmon enhanced fluorescence method, as a method having the effect of enhancing the electric field of the sensor unit, a method using the electric field enhancement effect by the optical waveguide mode is proposed in Non-Patent Document 2. . In this optical waveguide mode enhanced fluorescence spectroscopy (OWF), a metal layer and an optical waveguide layer made of a dielectric or the like are sequentially formed on a sensor portion, and an optical waveguide mode is formed on the optical waveguide layer. The fluorescence signal is enhanced by the electric field enhancement effect.

  Further, in Patent Document 2 and Non-Patent Document 3, instead of detecting fluorescence from a fluorescent label as in the fluorescence method described above, the fluorescence is generated by newly inducing surface plasmon in the metal layer. A method for detecting synchrotron radiation (SPCE: Surface Plasmon-Coupled Emission) has been proposed.

  As described above, various methods have been proposed as measurement methods in biomeasurement and the like.

JP-A-10-307141 US Patent Application Publication No. 2005/0053974

W. Knoll et al., Analytical Chemistry 77 (2005), p.2426-2431 2007 Spring Japan Society of Applied Physics Proceedings No.3, P.1378 Thorsten Liebermann Wolfgang Knoll, "Surface-plasmon field-enhanced fluorescence spectroscopy" Colloids and Surfaces A 171 (2000) 115-130

  In particular, in bio-measurement, the measurement result is strongly influenced by the temperature of the analysis chip. Therefore, in the measurement apparatus as described above, a temperature adjusting means for keeping the temperature of the analysis chip constant is generally provided.

  This temperature adjustment means is composed of a temperature adjustment member such as an aluminum block and a temperature control means for controlling the temperature of this temperature adjustment member, and the analysis chip placed in the measurement device is adjusted at the measurement position. It is moved inside the apparatus so as to be in contact with the member.

  In order to simplify the configuration of the measurement device, the analysis chip may be moved to a predetermined measurement position on the temperature adjustment member by sliding the analysis chip on the upper surface of the temperature adjustment member. If condensation occurs on the temperature adjusting member, when the analysis chip is slid on the temperature adjusting member, water droplets are collected by the analysis chip and carried to the measurement position. If this water droplet hinders fluorescence detection or the like, an error may occur in the measurement result.

  The present invention has been made in view of the above problems, and uses a photodetection method for analyzing a test substance by detecting light generated from a photoresponsive label substance bound to the test substance in a sensor portion of an analysis chip. It is an object of the present invention to provide a measuring apparatus that does not affect the measurement result even when condensation occurs on a temperature adjusting member for adjusting the temperature of the analysis chip.

  The measuring device of the present invention is a measuring device using a photodetection method for analyzing a test substance by detecting light generated from a photoresponsive labeling substance bonded to the test substance in a sensor part of an analysis chip, A temperature adjusting member for adjusting the temperature of the analysis chip by contacting the analysis chip at a predetermined measurement position on the upper surface, and a temperature control means for controlling the temperature of the temperature adjusting member, the upper surface of the temperature adjusting member Is provided with a slit for collecting water droplets collected as the object slides on the upper surface.

  In the measurement apparatus of the present invention, the analysis chip loaded from outside moves the analysis chip moving means to the measurement position by sliding the upper surface of the temperature adjusting member, or the analysis chip loaded from the outside is measured at the measurement position. And an analysis chip moving means having a sliding portion that slides on the upper surface of the temperature adjusting member.

  Moreover, it is preferable that a slit shall be extended in directions other than the direction parallel to a sliding direction.

  Moreover, it is preferable that a slit shall be extended in directions other than the direction orthogonal to a sliding direction.

  The width of the slit is preferably 1 mm or less.

  Further, the inside of the slit is preferably hydrophilic.

  Moreover, it is preferable that the slit is provided at a position that does not overlap the sensor unit when the analysis chip is at the measurement position.

  Moreover, it is preferable that the temperature adjusting member includes a water droplet collection hole that communicates with the slit and penetrates in the vertical direction.

  In this case, measurement light irradiating means for irradiating measurement light toward the water droplet collection hole from either above or below the water droplet collection hole, and measurement for detecting the measurement light either above or below the water droplet collection hole You may provide a light detection means and the judgment means which judges the presence or absence of the water in a water droplet collection | recovery hole based on the detection result by a measurement light detection means.

  In this case, the inside of the water droplet collection hole is preferably made hydrophobic.

  According to the measuring apparatus of the present invention, in the measuring apparatus using the photodetection method for analyzing the test substance by detecting light generated from the photoresponsive labeling substance bound to the test substance in the sensor part of the analysis chip, A temperature adjusting member for adjusting the temperature of the analysis chip by contacting the analysis chip at a predetermined measurement position on the upper surface, and a temperature control means for controlling the temperature of the temperature adjusting member, the upper surface of the temperature adjusting member In addition, since a slit for collecting water droplets collected as the object slides on the upper surface is provided, even if condensation occurs on the temperature adjusting member, some object is slid on the upper surface of the temperature adjusting member. Since the water droplets can be collected by moving, it is possible to prevent condensation from affecting the measurement results.

  In the measurement apparatus of the present invention, the analysis chip moving means for moving the analysis chip loaded from the outside to the measurement position by sliding the upper surface of the temperature adjusting member, or the analysis chip loaded from the outside to the measurement position In addition to the movement of the analysis chip moving means by using the analysis chip moving means normally provided in the apparatus by further providing an analysis chip moving means having a sliding portion that slides on the upper surface of the temperature adjusting member. Therefore, the present invention can be realized at low cost.

  Also, if the slit is extended in a direction parallel to the sliding direction, water droplets can hardly be collected, so by extending the slit in a direction other than the direction parallel to the sliding direction, Water droplet collection efficiency can be improved.

  Further, by making the slit extend in a direction other than the direction orthogonal to the sliding direction, it becomes easier to move the water droplets along with the sliding of the analysis chip, so that the recovery efficiency of the water droplets can be improved.

  In addition, by reducing the width of the slit to 1 mm or less, it is possible to minimize the reduction of the contact area with the analysis chip while collecting water droplets, so that the influence of the slit during temperature adjustment is minimized. be able to.

  Further, by making the inside of the slits hydrophilic, water droplets can easily move into the slits, so that the recovery efficiency of the water droplets can be improved.

  In addition, when the analysis chip is at the measurement position, the slit is provided at a position that does not overlap the sensor unit, so that the influence of the slit at the time of the main measurement can be minimized.

  Moreover, the temperature adjusting member is provided with a water droplet recovery hole that communicates with the slit and penetrates in the vertical direction, whereby the accumulated water droplets can be recovered efficiently.

  In this case, measurement light irradiating means for irradiating measurement light toward the water droplet collection hole from either above or below the water droplet collection hole, and measurement for detecting the measurement light either above or below the water droplet collection hole Since it is possible to detect that dew condensation has occurred inside the measuring apparatus by providing the light detection means and the determination means for determining the presence or absence of water in the water droplet collection hole based on the detection result by the measurement light detection means, It is possible to assist the appropriate operation of the measuring device, such as issuing a warning to the user.

  In this case, by making the inside of the water droplet collection hole hydrophobic, it becomes easy for the water droplet to block the inside of the water droplet collection hole, so that the water in the water droplet collection hole can be easily detected.

Schematic diagram of a fluorescence detection apparatus which is an embodiment of the measurement apparatus of the present invention Block diagram of the fluorescence detection device Schematic diagram showing an example of an analysis chip used in the fluorescence detection device Schematic diagram of temperature adjusting member and its periphery in the fluorescence detection device Top view of temperature adjusting member in the fluorescence detection device Sectional view taken along line VI-VI in FIG. FIG. 2 is a schematic diagram showing how a sample is extracted from a sample container using a nozzle tip by the sample processing means of FIG. FIG. 2 is a schematic diagram showing how the sample in the nozzle tip is injected and stirred into the reagent cell by the sample processing means of FIG. The schematic diagram which shows an example of the analysis chip in the said fluorescence detection apparatus, light irradiation means, and fluorescence detection means The graph which shows an example of the measurement result by the said fluorescence detection apparatus

  Hereinafter, a fluorescence detection apparatus according to an embodiment of the measurement apparatus of the present invention will be described in detail with reference to the drawings. FIG. 1 is a schematic diagram of a fluorescence detection apparatus according to an embodiment of the measurement apparatus of the present invention, FIG. 2 is a block diagram of the fluorescence detection apparatus, and FIG. 3 is a schematic diagram illustrating an example of an analysis chip used in the fluorescence detection apparatus. 4 is a schematic diagram of the temperature adjusting member and its surroundings in the fluorescence detection device, FIG. 5 is a top view of the temperature adjustment member in the fluorescence detection device, FIG. 6 is a sectional view taken along the line VI-VI in FIG. FIG. 7 is a schematic diagram showing a state in which a sample is extracted from a sample container using a nozzle tip by the sample processing means of FIG. 2, and FIG. FIG. 9 is a schematic view showing an example of stirring, FIG. 9 is a schematic view showing an example of the analysis chip, light irradiation means and fluorescence detection means in the fluorescence detection device, and FIG. 10 is a graph showing an example of measurement results by the fluorescence detection device. is there.

  This fluorescence detection device 1 is an immunological analysis device using surface plasmon resonance, and when performing analysis by the fluorescence detection device 1, a sample container CB containing the sample shown in FIG. 1 and a sample and a reagent are extracted. The nozzle chip NC used at the time, and the analysis chip 10 in which the reagent cell and the microchannel are formed are loaded. Note that the sample container CB, the nozzle tip NC, and the analysis chip 10 are all disposable items that are discarded once they are used. Then, the fluorescence detection apparatus 1 performs a quantitative or qualitative analysis on the test substance in the sample while flowing the sample through the microchannel 15 of the analysis chip 10.

  The fluorescence detection apparatus 1 includes a sample processing unit 20, a light irradiation unit 30, a fluorescence detection unit 40, a light irradiation unit 50, a light detection unit 60, an aluminum block (temperature adjusting member) 70, a temperature control unit 80, and a data analysis unit. 90 grade. The sample processing means 20 extracts a sample from the sample container CB containing the sample using the nozzle chip NC, and generates a sample solution obtained by mixing and stirring the extracted sample with a reagent.

  FIG. 3 is a schematic diagram illustrating an example of the analysis chip 10. The analysis chip 10 has a structure in which an inlet 12, an outlet 13, sample cells 14 a and 14 b, and a flow path 15 are formed in a main body 11 made of a dielectric plate such as a light transmissive resin. The injection port 12 communicates with the discharge port 13 via the flow channel 15, and by applying a negative pressure from the discharge port 13, the specimen is injected from the injection port 12, flows into the flow channel 15, and is discharged from the discharge port 13. The The sample cells 14a and 14b are containers for storing a fluorescent reagent (second antibody) to be mixed with the specimen in the specimen container CB. Note that the openings of the sample cells 14a and 14b are sealed with a sealing member, and the sealing member is pierced when the specimen and the fluorescent reagent are mixed.

  Further, a test region TR as a sensor unit for detecting a test substance in the specimen and a control region CR provided on the downstream side of the test region TR are formed in the flow channel 15. A first antibody is immobilized on the test region TR, and the labeled antibody is captured by a so-called sandwich method. In addition, a reference antibody is fixed to the control region CR, and the reference antibody captures the fluorescent substance when the sample solution flows on the control region CR. Two control regions CR are formed, a so-called negative control region CR for detecting non-specific adsorption, and a so-called positive control region CR for detecting a difference in reactivity due to a difference in specimen. Is formed.

  FIG. 4 is a schematic view of a temperature adjusting member and its surroundings in the fluorescence detection apparatus. The aluminum block 70 is for adjusting the temperature of the analysis chip 10 by contacting the analysis chip 10 at a predetermined measurement position on the upper surface, and the temperature control means 80 is for controlling the temperature of the aluminum block 70. is there. The analysis chip 10 loaded from outside is provided with an analysis chip moving means (not shown) that moves the upper surface of the aluminum block 70 to the measurement position.

  FIG. 5 is a top view of the aluminum block 70 in the fluorescence detection apparatus. A slit 71 is provided on the upper surface of the aluminum block 70 for collecting water droplets collected when the analysis chip 10 slides on the upper surface. The aluminum block 70 is provided with a water droplet collection hole 72 that communicates with the slit 71 and penetrates in the vertical direction. The analysis chip 10 is slid from the upper side to the lower side in FIG. 5 and moved to the measurement position. At this time, condensation occurs on the aluminum block 70, and the water collected by the slit 71 when the analysis chip 10 slides on the upper surface finally moves into the water droplet collection hole 72.

  The slits 71 are parallel to a plurality of slits 71a extending in a direction inclined from a direction orthogonal to the sliding direction of the analysis chip 10 (up and down direction in FIG. 5) and an end side (lower side in FIG. 5) on the light irradiation means 30 side. And a plurality of slits 71 a and a slit 71 c for communicating the slits 71 b with the water droplet collection hole 72.

  The slit 71a is formed so as to extend in a direction inclined from a direction orthogonal to the sliding direction of the analysis chip 10, and this facilitates the movement of the water droplet as the analysis chip 10 slides. The recovery efficiency can be improved.

  The slit 71b is formed so as to extend in parallel to the end side on the light irradiation means 30 side from the position of the sensor unit (CR / TR) when the analysis chip 10 is at the measurement position. Since the water droplets collected from when the tip of 10 passes the immediately preceding slit 71a until reaching the measurement position can be finally collected, it is possible to prevent water droplets from adhering to the excitation light incident surface of the analysis chip 10. .

  The widths of the slits 71a to 71c are formed to be 1 mm or less, and this makes it possible to minimize the decrease in the contact area with the analysis chip 10 while collecting water droplets. The influence of the slit can be minimized.

  The depths of the slits 71a to 71c are also formed to be 1 mm or less. This makes it possible to minimize the decrease in the volume of the aluminum block 70 while collecting water droplets. The impact can be minimized.

  Moreover, since the inside of the slits 71a to 71c is processed to be hydrophilic, and this makes it easier for the water droplet to move into the slit, the recovery efficiency of the water droplet can be improved.

  In FIG. 5, the position of the sensor unit (CR / TR) when the analysis chip 10 is at the measurement position is indicated by a dotted line. As shown in FIG. Is provided at a position that does not overlap the sensor section. Thereby, the influence of the slit at the time of this measurement can be suppressed to the minimum.

  6 is a cross-sectional view taken along line VI-VI in FIG. In the fluorescence detection apparatus 1 of the present embodiment, a light irradiation means 50 such as an LED and a light detection means 60 are arranged so as to sandwich the analysis chip 10 and the aluminum block 70. This is to see whether the tip of the sample solution has reached by light transmission. For example, when light is irradiated from the light irradiation means 50 from the lower side to the upper side of the analysis chip 10 and the amount of light is detected by the upper light detection means 60, if the sample solution has not reached the arrival measurement position, A part of light (about 4%) is reflected at the boundary between the lower member 11 and the flow path and the boundary between the flow path and the upper member 12 constituting the road member, and finally the light quantity is about 8%. When the sample solution has reached the measurement end position, the refractive index of the sample solution and the transparent resin is so close that no light is reflected, so that the amount of light hardly decreases. Therefore, when the sample solution has reached the arrival measurement position, the detected light amount is about 8% higher than when the sample solution has not reached. In this way, it is possible to know whether the tip of the sample solution has reached the arrival measurement position (that is, whether all of the sensor sections have passed) due to the change in the detected light quantity.

  Further, the water droplet collection hole 72 formed in the aluminum block 70 is provided at a position corresponding to the arrival measurement position by the light irradiation means 50 and the light detection means 60, and the light irradiation means 50 and the light detection means 60 are provided. The presence / absence of water in the water droplet collection hole 72 can also be confirmed by use.

  This confirmation is performed independently of the above-described confirmation of the arrival of the sample solution. Light is irradiated from the light irradiation means 50 from below to above the water droplet collection hole 72, and the upper light detection means 60 When the amount of light is detected, if there is water in the water droplet collection hole 72, light is absorbed and scattered by the water and the amount of light is reduced. If there is no water in the water droplet collection hole 72, the amount of light is reduced. Absent. Therefore, the presence or absence of water in the water droplet collection hole 72 can be known from the change in the detected light amount.

  The data analysis means 90 has a function as a judgment means for judging the presence or absence of water in the water droplet collection hole based on the detection result by the light detection means 60. The data analysis means 90 has water, that is, the fluorescence detection device 1 When it is determined that condensation has occurred inside, a warning is displayed or an alarm is sounded to notify the user.

  In addition, since the inside of the water droplet collection hole 72 is processed so as to be hydrophobic, and the water droplet easily closes the inside of the water droplet collection hole, the water in the water droplet collection hole 72 can be easily detected.

  When the start of analysis is instructed after the analysis chip 10 is placed at the measurement position, the sample processing means 20 aspirates the sample from the sample container CB using the nozzle chip NC as shown in FIG. Thereafter, as shown in FIG. 8, the sample processing means 20 pierces the seal member of the sample cell 14a, mixes and stirs the sample with the reagent in the sample cell 14a, and then sucks the sample solution again using the nozzle tip NC. . This operation is similarly performed for the sample cell 14b. Then, a sample solution is generated in which the second antibody B2, which is a second binding substance specifically binding in the reagent, to the test substance (antigen) A present in the sample is modified on the surface. Then, the sample processing means 20 installs the nozzle chip NC containing the sample solution on the injection port 12, and the sample solution in the nozzle chip NC flows into the flow path 15 by the negative pressure from the discharge port 13.

  In the present embodiment, the sample processing means 20 is illustrated as supplying the sample solution in which the sample and the reagent are mixed into the flow channel 15. However, the reagent is filled in the flow channel 15 in advance. The sample processing means 20 may allow only the sample to flow from the inlet 12.

  FIG. 9 is a schematic diagram showing an example of an analysis chip, light irradiation means, and fluorescence detection means. In FIG. 9, description will be given focusing on the test region TR, but the excitation light L is similarly applied to the control region CR.

  Specifically, the main body 11 of the analysis chip 10 includes a base 11a and an upper lid 11b formed of a dielectric material such as a light transmissive resin. The substrate (dielectric plate) 11a is provided with a groove for forming a flow path 15 (sample liquid holding portion), and an upper lid 11b is attached on the groove so that the groove portion becomes the flow path 15 (sample liquid). Functions as a holding unit). A metal film 16 is laminated on the bottom surface of the test region TR (same for the control region CR) of the flow path 15.

  The light irradiating means 30 irradiates the dielectric plate 17 and the metal film 16 in the test region TR through the prism with the excitation light L from the back side of the analysis chip 10 at an incident angle that is a total reflection condition. The fluorescence detection means 40 is composed of, for example, a CCD, a CMOS, etc., and obtains an image signal FS by photographing the test area TR.

  Then, the excitation light L is incident on the interface between the dielectric plate 17 and the metal film 16 at a specific incident angle equal to or greater than the total reflection angle by the light irradiation means 30, thereby entering the sample S on the metal film 16. The evanescent wave Ew oozes out, and surface plasmons are excited in the metal film 16 by the evanescent wave Ew. This surface plasmon causes an electric field distribution on the surface of the metal film 16 to form an electric field enhancement region. Then, the fluorescent labeling substance F bound to the first antibody B1 fixed on the metal film 16 is excited by the evanescent wave Ew to generate enhanced fluorescence.

  In detection using plasmon enhancement, metal quenching may occur and the sensitivity may be lowered. For example, if a quenching prevention layer made of a silica layer, a polystyrene layer, or the like is provided on the metal layer 16, Such a problem can be solved. In addition, as for the fluorescent labeling substance F, for example, as a quenching preventive substance such as a fluorescent dye encapsulated in polystyrene particles or silica particles, or a gold colloid surface coated with polystyrene, the problem of metal quenching is solved. be able to.

  The data analysis means 90 in FIG. 2 analyzes the test substance based on the temporal change of the fluorescence signal FS detected by the fluorescence detection means 40. Specifically, since the fluorescence intensity changes depending on the amount of the fluorescent labeling substance F bound thereto, the fluorescence intensity changes with time as shown in the graph of FIG. The data analysis means 90 acquires a plurality of fluorescent signals FS in a predetermined sampling period (for example, 5 minutes) at a predetermined sampling period (for example, a period of 5 seconds), and analyzes the change rate of the fluorescence intensity over time to analyze the test in the sample. Perform quantitative analysis of substances (rate method). Note that the results obtained as described above may be further corrected using the measurement results in the control region CR.

  The finally obtained analysis result is output from the information output means 4 comprising a monitor, a printer or the like.

  As mentioned above, although preferable embodiment of this invention was described, this invention is not limited to the said embodiment.

  For example, the number and arrangement shape of the slits 71 are not limited to the above, and any mode may be used.

  Further, the depth of the slit 71 is not limited to the above, and may be a depth of 1 mm or more, and does not need to be a uniform depth. For example, the depth of the slit 71 is increased toward the water droplet collection hole 72. If so, the water droplets in the slit 71 can be more easily moved to the water droplet collection hole 72.

  In this embodiment, the analysis chip 10 is slid on the aluminum block 70 and moved to the measurement position by the analysis chip moving means. However, the analysis chip 10 is not necessarily moved automatically to the measurement position. Instead, the operator may manually slide the analysis chip 10 and move it to the measurement position. Even if the operator manually slides the analysis chip 10, the water droplets are collected by the slit 71 as described above.

  In the present embodiment, when the analysis chip 10 slides on the aluminum block 70, water droplets are collected by the slit 71. However, the analysis chip 10 does not necessarily slide on the aluminum block 70. . For example, the analysis chip moving means includes a sliding portion that slides on the aluminum block 70, and when the analysis chip 10 is moved to the measurement position, the sliding portion slides on the aluminum block 70 and drops of water by the slit 71. May be recovered. In this case, the analysis chip 10 does not necessarily have to slide on the aluminum block 70.

  In addition to the surface plasmon enhanced fluorescence method and evanescent fluorescence method, the fluorescence detection apparatus of the present invention can be adapted to various systems such as an optical waveguide mode enhanced fluorescence spectroscopy.

  In addition to the above, it goes without saying that various improvements and modifications may be made without departing from the scope of the present invention.

DESCRIPTION OF SYMBOLS 1 Fluorescence detection apparatus 10 Analytical chip 20 Sample processing means 30 Light irradiation means 40 Fluorescence detection means 50 Light irradiation means 60 Light detection means 70 Aluminum block (temperature adjustment member)
71 Slit 72 Water droplet collection hole 80 Temperature control means 90 Data analysis means CR Control area FS Fluorescence signal L Excitation light TR Test area

Claims (11)

A measuring device using a photodetection method for detecting light generated from a photoresponsive labeling substance bonded to a substance to be detected in a sensor part of an analysis chip and analyzing the substance to be tested,
A temperature adjusting member for adjusting the temperature of the analysis chip by contacting the analysis chip at a predetermined measurement position on the upper surface;
Temperature control means for controlling the temperature of the temperature adjusting member,
The measuring apparatus according to claim 1, wherein a slit for collecting water droplets collected as an object slides on the upper surface is provided on the upper surface of the temperature adjusting member.   2. The measuring apparatus according to claim 1, further comprising an analyzing chip moving means for moving the analyzing chip loaded from the outside to the measuring position by sliding the upper surface of the temperature adjusting member.   2. The analysis chip moving means for moving the analysis chip loaded from the outside to the measurement position and having a sliding portion that slides on the upper surface of the temperature adjusting member. Measuring device.   The measuring apparatus according to claim 1, wherein the slit extends in a direction other than a direction parallel to the sliding direction.   The measuring apparatus according to claim 1, wherein the slit extends in a direction other than a direction orthogonal to the sliding direction.   The measuring apparatus according to claim 1, wherein a width of the slit is 1 mm or less.   The measuring apparatus according to claim 1, wherein the inside of the slit is hydrophilic.   The measurement device according to claim 1, wherein the slit is provided at a position that does not overlap the sensor unit when the analysis chip is at the measurement position.   9. The measuring apparatus according to claim 1, wherein the temperature adjusting member includes a water droplet collecting hole that communicates with the slit and penetrates in the vertical direction. A measurement light irradiating means for irradiating measurement light from either above or below the water droplet recovery hole toward the water droplet recovery hole;
A measurement light detection means for detecting the measurement light at either the upper side or the lower side of the water droplet recovery hole;
The measuring apparatus according to claim 9, further comprising a determination unit that determines the presence or absence of water in the water droplet collection hole based on a detection result of the measurement light detection unit.   The measuring apparatus according to claim 10, wherein the inside of the water droplet collection hole is hydrophobic.

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