JP2013071897A5 - - Google Patents

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JP2013071897A5
JP2013071897A5 JP2011210463A JP2011210463A JP2013071897A5 JP 2013071897 A5 JP2013071897 A5 JP 2013071897A5 JP 2011210463 A JP2011210463 A JP 2011210463A JP 2011210463 A JP2011210463 A JP 2011210463A JP 2013071897 A5 JP2013071897 A5 JP 2013071897A5
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bonito
amino acid
dipeptide
acid sequence
trp
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JP2011210463A
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JP5417405B2 (en
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鰹、鰹荒節、鰹枯節、宗田鰹、宗田鰹節、鰯、鰯節、鯵、鯵節、鯖、鯖節、煮干またはその他雑節の魚肉性タンパク質由来の、アンジオテンシン変換酵素阻害活性を有するジペプチドを含有する組成物であって、Dipeptides with angiotensin-converting enzyme inhibitory activity derived from fish-like proteins of bonito, arabic bonito, bonito bonito, soda bonito, soda bonito, bonito, bonito, bonito, bonito, bonito, bonito, niboshi or other miscellaneous knots A composition comprising:
Ｔｒｐ−Ｌｅｕのアミノ酸配列から成るジペプチド、A dipeptide consisting of the amino acid sequence of Trp-Leu,
Ｌｅｕ−Ｔｒｐのアミノ酸配列から成るジペプチド、A dipeptide consisting of the amino acid sequence of Leu-Trp,
Ｔｒｐ−Ｉｌｅのアミノ酸配列から成るジペプチド、A dipeptide consisting of the amino acid sequence of Trp-Ile,
Ｖａｌ−Ｔｙｒのアミノ酸配列から成るジペプチド及びA dipeptide consisting of the amino acid sequence of Val-Tyr, and
Ｔｒｐ−Ａｓｎのアミノ酸配列から成るジペプチド及び／又はA dipeptide consisting of the amino acid sequence of Trp-Asn and / or
それらの酸付加塩Their acid addition salts
を含有する組成物。A composition containing 請求項１に記載の組成物を含有する加工食品又は特定保健用食品。Processed food or food for specified health use containing the composition according to claim 1. Ｔｒｐ−Ｌｅｕのアミノ酸配列から成るジペプチド、Ｌｅｕ−Ｔｒｐのアミノ酸配列から成るジペプチド、Ｔｒｐ−Ｉｌｅのアミノ酸配列から成るジペプチド、Ｖａｌ−Ｔｙｒのアミノ酸配列から成るジペプチド及びＴｒｐ−Ａｓｎのアミノ酸配列から成るジペプチド及び／又はそれらの酸付加塩を含有する組成物の製造方法であって、
１）鰹、鰹荒節、鰹枯節、宗田鰹、宗田鰹節、鰯、鰯節、鯵、鯵節、鯖、鯖節、煮干またはその他雑節の魚肉性タンパク質を熱水で抽出し、
２）その熱水抽出後に残留する水不溶性タンパク質を粉砕し、得られた粉砕物を水分に分散して該水不溶性タンパク質の水分散液を作り、該水分散液中で、分散された水不溶性タンパク質の粒子に、プロテアーゼをｐＨ５．０〜９．０の至適条件下に４０〜６０℃の温度で反応させ、これにより該水不溶性タンパク質の酵素的加水分解を行い、その後、酵素反応を停止させ、そして得られた含水の加水分解反応混合物から水不溶性の粒子を除去し、これにより、疎水性・親水性高分子・低分子プチドおよび水溶性アミノ酸を含む水溶液を収得し、
３）該水溶液から疎水性樹脂カラム法により得られた吸着画分をさらに限外ろ過（分子量１０００）に負荷し透過画分の組成物を分離することから成る、製造方法。 A dipeptide comprising the amino acid sequence of Trp-Leu, a dipeptide comprising the amino acid sequence of Leu-Trp, a dipeptide comprising the amino acid sequence of Trp-Ile, a dipeptide comprising the amino acid sequence of Val-Tyr, and a dipeptide comprising the amino acid sequence of Trp-Asn and A method for producing a composition containing an acid addition salt thereof,
1) Extract fish-like protein from bonito, bonito, bonito, soda, soda, bonito, bonito, bonito, bonito, bonito, bonito, boiled or other miscellaneous fish with hot water,
2) The water-insoluble protein remaining after the hot water extraction is pulverized, and the obtained pulverized product is dispersed in moisture to form an aqueous dispersion of the water-insoluble protein. Protease is reacted with protein particles at a temperature of 40-60 ° C. under optimum conditions of pH 5.0-9.0, thereby enzymatically hydrolyzing the water-insoluble protein, and then the enzymatic reaction is stopped. And removing water-insoluble particles from the resulting hydrous hydrolysis reaction mixture, thereby obtaining an aqueous solution containing a hydrophobic / hydrophilic polymer / low molecular peptide and a water-soluble amino acid,
3) A production method comprising further subjecting an adsorbed fraction obtained from the aqueous solution by a hydrophobic resin column method to ultrafiltration (molecular weight 1000) to separate the composition of the permeated fraction. 前記プロテアーゼは、食品工業用のプロテアーゼである、請求項３に記載のジペプチドの製造方法。   The method for producing a dipeptide according to claim 3, wherein the protease is a protease for food industry. 前記プロテアーゼは、プロチンＮＹ１００（天野エンザイム）及びサモアーゼＰＣ１０Ｆ（大和化成）より選択される、請求項３又は４に記載のジペプチドの製造方法。   The method for producing a dipeptide according to claim 3 or 4, wherein the protease is selected from protin NY100 (Amano Enzyme) and Samoaase PC10F (Daiwa Kasei). 経口単回投与実験で１単位中０．０００１ｍｇ〜１ｍｇ／ｋｇの投与量で請求項１に記載の組成物を含むアンジオテンシン変換酵素阻害剤と血圧降下剤。 An angiotensin converting enzyme inhibitor and an antihypertensive agent comprising the composition according to claim 1 at a dose of 0.0001 mg to 1 mg / kg per unit in a single oral administration experiment. 請求項１に記載される組成物を配合してあることを特徴とする、飲食料。 Characterized in that are mixed with compositions according to claim 1, food and beverage.
JP2011210463A 2011-09-27 2011-09-27 Method for producing angiotensin converting enzyme inhibitory antihypertensive peptide composition Active JP5417405B2 (en)

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WO2014002571A1 (en) * 2012-06-26 2014-01-03 ヤマキ株式会社 Angiotensin-converting-enzyme inhibiting dipeptide
WO2019006955A1 (en) * 2017-07-07 2019-01-10 广州世优生物科技有限公司 Application of acid amide dipeptide for preparing antihypertensive drug or health care product
CN113929734B (en) * 2021-09-22 2023-09-05 湖北泓肽生物科技有限公司 Synthesis method of dipeptide-2
CN113912673B (en) * 2021-11-09 2024-02-09 河南省农业科学院 Sesame-derived low-bitter ACE (angiotensin converting enzyme) inhibitory peptide as well as preparation method and application thereof
CN115197309A (en) * 2022-07-15 2022-10-18 广东南兴天虹果仁制品有限公司 Amygdalus communis protein-derived ACE inhibitory peptide, and preparation method and application thereof
CN117384239B (en) * 2023-12-12 2024-02-09 深圳保时健生物工程有限公司 Anti-aging salmon roe tripeptide as well as preparation method and application thereof

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JP3592593B2 (en) * 1999-10-15 2004-11-24 日本合成化学工業株式会社 Angiotensin converting enzyme inhibitor
EP2161029A1 (en) * 2008-09-09 2010-03-10 Unilever N.V. Composition comprising peptides
JP5416964B2 (en) * 2008-12-26 2014-02-12 ヤマキ株式会社 Novel tripeptides, methods for producing these tripeptides, and methods for producing angiotensin converting enzyme inhibitors

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