JP2013068462A5 - - Google Patents
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- JP2013068462A5 JP2013068462A5 JP2011205938A JP2011205938A JP2013068462A5 JP 2013068462 A5 JP2013068462 A5 JP 2013068462A5 JP 2011205938 A JP2011205938 A JP 2011205938A JP 2011205938 A JP2011205938 A JP 2011205938A JP 2013068462 A5 JP2013068462 A5 JP 2013068462A5
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Claims (8)
前記装着部に前記反応容器を装着した場合に、前記流路に対して、前記反応液が移動する方向に温度勾配を形成する温度勾配形成部と、
前記装着部に前記反応容器を装着した場合に、前記装着部及び前記温度勾配形成部の配置を、第１の配置と、重力の作用する方向における前記流路の最下点の位置が前記第１の配置とは異なる第２の配置と、の間で切換える駆動機構と、
前記反応容器内からの前記所定の波長の光の強度を検出する検出部と、
前記駆動機構を制御する制御部と、
を含み、
前記制御部は、
前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置と前記第２の配置との間で切換えさせるタイミングである切換タイミングと、
前記検出部によって検出された前記光の強度が基準値を跨いで変化するタイミングである検出タイミングと、に基づいて、
前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置及び前記第２の配置のうち少なくとも一方に保持させる時間である保持時間を決定する、熱サイクル装置。 The reaction liquid, different specific gravity from that of the reaction liquid, and the liquid immiscible with the reaction liquid is filled, either the liquid immiscible with the reaction solution and the reaction solution is, light of a predetermined wavelength a mounting portion which includes a fluorescent substance, attaching the reaction vessel containing a flow path in which the reaction solution is moved to emit,
A temperature gradient forming unit that forms a temperature gradient in a direction in which the reaction solution moves with respect to the flow path when the reaction vessel is mounted on the mounting unit;
When the reaction vessel is attached to the attachment part, the arrangement of the attachment part and the temperature gradient forming part is the first arrangement, and the position of the lowest point of the flow path in the direction in which gravity acts is the first position. a drive mechanism for switching between a different second configuration to the first configuration,
A detection unit for detecting the intensity of light of the predetermined wavelength from within the reaction vessel;
A control unit for controlling the drive mechanism;
Including
The controller is
A switching timing that is a timing for switching the placement of the mounting portion and the temperature gradient forming portion between the first placement and the second placement by the driving mechanism;
Based on the detection timing, which is the timing at which the intensity of the light detected by the detection unit changes across a reference value,
A thermal cycle device that determines a holding time that is a time for holding the placement of the mounting portion and the temperature gradient forming portion in at least one of the first placement and the second placement by the driving mechanism.
前記制御部は、
前記切換タイミングから前記検出タイミングまでの時間を計測する計測手段と、
前記計測手段によって計測された前記時間に基づいて、前記保持時間を決定する決定手段と、
を含む、熱サイクル装置。 The thermal cycle apparatus according to claim 1, wherein
The controller is
Measuring means for measuring the time from the switching timing to the detection timing;
Determining means for determining the holding time based on the time measured by the measuring means;
Including a heat cycle device.
する蛍光物質を含み、前記反応液が移動する流路を含む反応容器を装着する装着部と、
前記装着部に前記反応容器を装着した場合に、前記流路に対して、前記反応液が移動する方向に温度勾配を形成する温度勾配形成部と、
前記装着部に前記反応容器を装着した場合に、前記装着部及び前記温度勾配形成部の配置を、第１の配置と、重力の作用する方向における前記流路の最下点の位置が前記第１の配置とは異なる第２の配置と、の間で切換える駆動機構と、
前記反応容器内からの前記所定の波長の光の強度を検出する検出部と、
前記駆動機構を制御する制御部と、
を含み、
前記制御部は、
前記検出部によって検出された前記光の強度が基準値を跨いで変化するタイミングである検出タイミングから所定の時間が経過するまで、前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置及び前記第２の配置のうち少なくとも一方に保持させる、熱サイクル装置。 The reaction liquid, different specific gravity from that of the reaction liquid, and the liquid immiscible with the reaction liquid is filled, either the liquid immiscible with the reaction solution and the reaction solution is, light of a predetermined wavelength a mounting portion which includes a fluorescent substance, attaching the reaction vessel containing a flow path in which the reaction solution is moved to emit,
A temperature gradient forming unit that forms a temperature gradient in a direction in which the reaction solution moves with respect to the flow path when the reaction vessel is mounted on the mounting unit;
When the reaction vessel is attached to the attachment part, the arrangement of the attachment part and the temperature gradient forming part is the first arrangement, and the position of the lowest point of the flow path in the direction in which gravity acts is the first position. a drive mechanism for switching between a different second configuration to the first configuration,
A detection unit for detecting the intensity of light of the predetermined wavelength from within the reaction vessel;
A control unit for controlling the drive mechanism;
Including
The controller is
Until the predetermined time has elapsed from the detection timing, which is the timing at which the intensity of the light detected by the detection unit changes across a reference value, the arrangement of the mounting unit and the temperature gradient forming unit is arranged by the drive mechanism. A thermal cycle device that is held in at least one of the first arrangement and the second arrangement.
前記検出部は、前記第１の配置となった場合に、前記流路内において重力の作用する方向における最下点を含む領域からの前記光の強度を検出する、熱サイクル装置。 In the heat cycle apparatus according to any one of claims 1 to 3,
The said detection part is a heat cycle apparatus which detects the intensity | strength of the said light from the area | region containing the lowest point in the direction which gravity acts in the said flow path when it becomes the said 1st arrangement | positioning.
前記反応容器に充填された前記反応液は、前記蛍光物質を含み、
前記制御部は、前記検出部によって検出された前記光の強度が前記基準値を上回ったタイミングを前記検出タイミングとする、熱サイクル装置。 In the heat cycle apparatus according to any one of claims 1 to 4,
The reaction solution filled in the reaction vessel contains the fluorescent substance,
The said control part is a thermal cycle apparatus which makes the detection timing the timing when the intensity | strength of the said light detected by the said detection part exceeded the said reference value.
前記反応容器に充填された前記反応液とは混和しない液体は、前記蛍光物質を含み、
前記制御部は、前記検出部によって検出された前記光の強度が前記基準値を下回ったタイミングを前記検出タイミングとする、熱サイクル装置。 In the heat cycle apparatus according to any one of claims 1 to 4,
The liquid immiscible with the reaction liquid filled in the reaction vessel contains the fluorescent substance,
The said control part is a thermal cycle apparatus which makes the said detection timing the timing when the intensity | strength of the said light detected by the said detection part fell below the said reference value.
前記装着部に前記反応容器を装着した場合に、前記流路に対して、前記反応液が移動する方向に温度勾配を形成する温度勾配形成部と、
前記装着部に前記反応容器を装着した場合に、前記装着部及び前記温度勾配形成部の配置を、第１の配置と、重力の作用する方向における前記流路の最下点の位置が前記第１の配置とは異なる第２の配置と、の間で切換える駆動機構と、
を含む熱サイクル装置の制御方法であって、
前記反応容器に充填された前記反応液及び前記反応液とは混和しない液体のいずれか一方は、所定の波長の光を発する蛍光物質を含み、
切換タイミングに基づいて、前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置と前記第２の配置との間で切換えさせることと、
前記反応容器内からの所定の波長の光の強度が基準値を跨いで変化するタイミングである検出タイミング検出することと、
前記切換タイミングと、前記検出タイミングとに基づいて、前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置及び前記第２の配置のうち少なくとも一方に保持させる時間である保持時間を決定することと、
を含む、熱サイクル装置の制御方法。 The reaction liquid, and the different specific gravity from the reaction liquid, the reaction liquid and the liquid immiscible is filled with a mounting portion for mounting a reaction vessel containing a flow path in which the reaction liquid is moved,
A temperature gradient forming unit that forms a temperature gradient in a direction in which the reaction solution moves with respect to the flow path when the reaction vessel is mounted on the mounting unit;
When the reaction vessel is attached to the attachment part, the arrangement of the attachment part and the temperature gradient forming part is the first arrangement, and the position of the lowest point of the flow path in the direction in which gravity acts is the first position. a drive mechanism for switching between a different second configuration to the first configuration,
A control method for a heat cycle apparatus including:
Either one of the reaction liquid filled in the reaction container and the liquid immiscible with the reaction liquid contains a fluorescent substance that emits light of a predetermined wavelength,
Based on the switching timing, the arrangement of the mounting part and the temperature gradient forming part is switched between the first arrangement and the second arrangement by the driving mechanism;
Detecting a detection timing that is a timing at which the intensity of light of a predetermined wavelength from within the reaction vessel changes across a reference value;
Based on the switching timing and the detection timing, the drive mechanism holds the placement of the mounting portion and the temperature gradient forming portion in at least one of the first placement and the second placement. Determining the retention time;
A control method for a thermal cycle device.
前記装着部に前記反応容器を装着した場合に、前記流路に対して、前記反応液が移動する方向に温度勾配を形成する温度勾配形成部と、
前記装着部に前記反応容器を装着した場合に、前記装着部及び前記温度勾配形成部の配置を、第１の配置と、重力の作用する方向における前記流路の最下点の位置が前記第１の配置とは異なる第２の配置と、の間で切換える駆動機構と、
を含む熱サイクル装置の制御方法であって、
前記反応容器に充填された前記反応液及び前記反応液とは混和しない液体のいずれか一方は、所定の波長の光を発する蛍光物質を含み、
前記反応容器内からの所定の波長の光の強度が基準値を跨いで変化するタイミングである検出タイミング検出することと、
前記検出タイミングから所定の時間を経過するまで、前記駆動機構によって前記装着部及び前記温度勾配形成部の配置を前記第１の配置及び前記第２の配置のうち少なくとも一方に保持させることと、
を含む、熱サイクル装置の制御方法。 The reaction liquid, and the different specific gravity from the reaction liquid, the reaction liquid and the liquid immiscible is filled with a mounting portion for mounting a reaction vessel containing a flow path in which the reaction liquid is moved,
A temperature gradient forming unit that forms a temperature gradient in a direction in which the reaction solution moves with respect to the flow path when the reaction vessel is mounted on the mounting unit;
When the reaction vessel is attached to the attachment part, the arrangement of the attachment part and the temperature gradient forming part is the first arrangement, and the position of the lowest point of the flow path in the direction in which gravity acts is the first position. a drive mechanism for switching between a different second configuration to the first configuration,
A control method for a heat cycle apparatus including:
Either one of the reaction liquid filled in the reaction container and the liquid immiscible with the reaction liquid contains a fluorescent substance that emits light of a predetermined wavelength,
Detecting a detection timing that is a timing at which the intensity of light of a predetermined wavelength from within the reaction vessel changes across a reference value;
Holding the arrangement of the mounting part and the temperature gradient forming part in at least one of the first arrangement and the second arrangement by the drive mechanism until a predetermined time has elapsed from the detection timing;
A control method for a thermal cycle device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011205938A JP5896110B2 (en) | 2011-09-21 | 2011-09-21 | Thermal cycle device and control method for thermal cycle device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011205938A JP5896110B2 (en) | 2011-09-21 | 2011-09-21 | Thermal cycle device and control method for thermal cycle device |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013068462A JP2013068462A (en) | 2013-04-18 |
| JP2013068462A5 true JP2013068462A5 (en) | 2014-10-09 |
| JP5896110B2 JP5896110B2 (en) | 2016-03-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011205938A Active JP5896110B2 (en) | 2011-09-21 | 2011-09-21 | Thermal cycle device and control method for thermal cycle device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5896110B2 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7163400B2 (en) * | 2018-09-27 | 2022-10-31 | 株式会社日立ハイテク | Reaction vessel for automatic analyzer |
| CN111474151B (en) * | 2020-04-18 | 2023-03-21 | 杭州准芯生物技术有限公司 | Liquid detection method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003102179A1 (en) * | 2002-05-31 | 2003-12-11 | Kankyo Engineering Co., Ltd. | Novel method of assyaing nucleic acid using labeled nucleotide |
| JP5196126B2 (en) * | 2007-12-10 | 2013-05-15 | セイコーエプソン株式会社 | Biological sample reaction apparatus and biological sample reaction method |
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