JP2013037007A - Microchannel plate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microchannel plate capable of simply and promptly carrying out the inspection of a specimen.SOLUTION: A microchannel plate 300 is provided with a first plate member 310, a first sheet member 320, a second plate member 330, and a second sheet member 340. A reagent and a specimen are mixed in a space including a first space formed in the first plate member 310 and a second space formed in the second plate member 340. The first sheet member 320 has a communication hoel 321 for communicating the first space and the second space. The second space includes reagent storage spaces 332 and 333 for storing reagent containers, a mixing space 331, a reagent channel, and an upstream side waste liquid channel. The first space includes a waste liquid collecting space 315, and a downstream side waste liquid channel. The second sheet member has pressing holes 342 and 343 enabling a user to directly press the reagent containers stored in the reagent storage spaces 332 and 333.

Description

  The present invention relates to a microchannel plate, and in particular, includes a plate member and a sheet member laminated on the surface of the plate member, and a reagent and a sample are mixed in a space in a recess formed on the surface of the plate member. The present invention relates to a microchannel plate.

  Conventionally, preparations are used for testing specimens such as cells and blood. Generally, a preparation is composed of a slide glass and a cover glass that covers a specimen placed on the surface of the slide glass, so that it is easily broken and requires care in handling. In the preparation, since the specimen and the reagent are mixed on the surface of the slide glass, it is necessary to hold the specimen on the surface of the slide glass or to remove the excessive reagent spread on the surface of the slide glass.

  In recent years, a microchannel plate using a plastic substrate has been developed (see, for example, Patent Document 1). In this microchannel plate, since the annular recess is formed in the central portion of the plastic substrate, the specimen can be mixed with the reagent in the recess without being fixed to the plastic substrate. In addition, since the micro flow path plate does not use a slide glass or a cover glass unlike the above-mentioned preparation, there is no problem caused by the slide glass or the cover glass.

Utility Model Registration No. 3056680

  However, in the microchannel plate described in Patent Document 1, no reagent is accommodated in the microchannel plate, and no space for accommodating the reagent is provided. In order to perform mixing, it was necessary to prepare a reagent separately from the microchannel plate. For this reason, the microchannel plate described in Patent Document 1 requires a lot of labor and time to inspect the specimen.

  The present invention has been made in view of the above problems, and an object of the present invention is to provide a micro-channel plate that can easily and quickly test a specimen.

  In order to solve the above-described problems, a microchannel plate according to the present invention includes a hard first plate member having a recess formed on a surface thereof, and a first sheet member laminated on the surface of the first plate member. A hard second plate member laminated on the surface of the first sheet member and having at least one through hole penetrating in the thickness direction and a recess formed on the front and back surfaces; and on the surface of the second plate member A reagent in a space including a first space in a recess formed in the first plate member, and a second space in the through hole and in the recess formed in the second plate member. The first sheet member has a communication hole that communicates the first space and the second space, and the second space is filled with a liquid reagent. Reagent storage space for storing reagent containers A mixing space in which the sample is stored and the sample and the reagent are mixed, a reagent flow path connecting the reagent storage space and the mixing space, and an upstream waste liquid flow path connecting the mixing space and the communication hole The first space includes a waste liquid storage space in which a waste liquid containing a reagent flowing out from the mixing space through the upstream side waste liquid flow path and the communication hole is stored, and a downstream connecting the communication hole and the waste liquid storage space. The second sheet member includes a side waste liquid flow path, and the second sheet member has a pressing hole that allows the reagent container accommodated in the reagent accommodating space to be directly pressed. The reagent container can be a blister pack.

  According to this configuration, since the reagent storage space for storing the reagent is provided in the first plate member, the reagent can be stored in the reagent storage space in advance. Further, according to this configuration, since the first space of the first plate member and the second space of the second plate member are connected, the reagent storage space and the waste liquid storage space can be obtained without increasing the area of the plate member. Etc., and more complicated flow paths can be formed. Furthermore, according to this structure, the reagent container accommodated in the reagent accommodating space can be pressed directly. Therefore, according to the microchannel plate according to the present invention, the specimen can be inspected easily and quickly.

  In the microchannel plate according to the present invention, the second plate member is formed such that the through hole and the recess formed in the second plate member communicate with the first through hole penetrating in the thickness direction and the first through hole. A first recess formed on the back surface, a second recess formed on the surface of the second plate member, a second through hole connecting the first recess and the second recess, the second recess and the first A reagent containing space comprising a space in the first through hole and in the first recess, a mixing space comprising the space in the second recess, and a reagent flow path. May comprise a space in the second through hole, and the upstream waste liquid flow path may comprise a space in the third through hole.

  Further, the second plate member has an air suction port formed by extending a concave portion on the surface of the second plate member to the end, and the second space is formed in a through hole formed in the second plate member. A pump space in which air is sucked and discharged, a space in a recess formed on the surface of the second plate member, an air suction flow path connecting the air suction port and the pump space, and the second plate An air discharge passage formed of a space in a recess formed on the surface of the member and connecting the pump space and the mixing space; and the first plate member can press a lower portion of the pump space of the first sheet member It is preferable to have a pressing hole, and a portion that covers the air suction flow path of the second sheet member is provided with a backflow prevention valve that prevents air from being discharged from the pump space to the air suction port. When the pump space lower part of the seat member and the pump space upper part of the second seat member are continuously pressed, the air sucked from the air suction port passes through the air suction flow path, the pump space, and the air discharge flow path. The waste liquid in the mixing space is preferably pushed out by the air and flows into the waste liquid storage space through the upstream waste liquid flow path and the downstream waste liquid flow path.

  According to these structures, since the pump space and the waste liquid reservoir space are provided, the pump space lower part of the first sheet member and the pump space upper part of the second sheet member are continuously pressed, so that the inside of the mixing space The waste liquid can be pushed out by the air sucked from the air suction port and allowed to flow into the waste liquid storage space. Thereby, it is possible to prevent the reagent from accumulating in the mixing space.

  Furthermore, the micro flow path plate according to the present invention further includes a hard back plate member laminated on the surface of the second sheet member, and the back plate member communicates with the pressing hole of the second sheet member and has a reagent containing space. It is preferable to have the 1st press hole which enables the reagent container accommodated in 1 to be directly pressed, and the 2nd press hole which can press the pump space upper part of a 2nd sheet | seat member.

  According to this configuration, the first sheet member is sandwiched between the hard first plate member and the hard second plate member, and the second sheet member is a hard second plate member and a hard back plate member. Therefore, it is possible to prevent the reagent from leaking between the first sheet member and the first plate member and between the second sheet member and the second plate member.

  Furthermore, it is preferable that the back plate member is transparent, and the second sheet member has an observation hole for observing the specimen in the upper part of the mixing space, thereby using a microscope from the surface of the back plate member. The specimen can be observed.

  Furthermore, it is preferable that the microchannel plate according to the present invention is provided with a filter that allows the waste liquid to pass through the upstream waste liquid flow path without allowing the specimen to pass therethrough.

  According to this configuration, the specimen can be prevented from flowing into the waste liquid storage space, and the specimen can be examined on the filter.

  Note that the term “elastomer” in the present specification is made of an elastic polymer material such as rubber or thermoplastic elastomer.

  ADVANTAGE OF THE INVENTION According to this invention, the microchannel plate which can test | inspect a sample simply and rapidly can be provided.

It is a microchannel plate concerning a 1st embodiment, (a) is a perspective view and (b) is an exploded perspective view. It is the microchannel plate which concerns on 2nd Embodiment, Comprising: (a) is a perspective view, (b) is a disassembled perspective view. It is a plate member of the micro channel plate concerning a 2nd embodiment, (a) is a top view, (b) is a bottom view, and (c) is an AA sectional view of (a). It is a sheet | seat member of the microchannel plate which concerns on 2nd Embodiment, Comprising: (a) is a top view, (b) is a bottom view, (c) is BB sectional drawing of (a). It is CC sectional drawing of Fig.4 (a), Comprising: (a) is a figure which shows the state which the backflow prevention valve closed, (b) is a figure which shows the state in which the backflow prevention valve opened. It is a backplate member of the microchannel plate concerning a 2nd embodiment, (a) is a top view, (b) is a bottom view, (c) is a DD sectional view of (a). It is the microchannel plate which concerns on 3rd Embodiment, Comprising: (a) is a perspective view, (b) is a disassembled perspective view. It is the 2nd plate member of the micro channel plate concerning a 3rd embodiment, (a) is a top view, (b) is a bottom view, (c) is an EE sectional view of (a), (d) ) Is an FF enlarged cross-sectional view of (a). FIG. 6 is a micro flow path plate according to a third embodiment, where (a) is a vicinity of an upstream waste liquid flow path, and (b) is a partial cross-sectional view of a reagent storage space. It is a 1st sheet | seat member of the microchannel plate which concerns on 3rd Embodiment, Comprising: (a) is a top view, (b) is a bottom view, (c) is GG sectional drawing of (a). It is a 1st plate member of the microchannel plate concerning a 3rd embodiment, and (a) is a top view, (b) is a bottom view, and (c) is a HH sectional view of (a). It is a 2nd sheet | seat member of the microchannel plate which concerns on 3rd Embodiment, Comprising: (a) is a top view, (b) is a bottom view, (c) is II sectional drawing of (a). It is a backplate member of the micro channel plate concerning a 3rd embodiment, and (a) is a top view, (b) is a bottom view, and (c) is a JJ sectional view of (a). It is a microchannel plate concerning a 4th embodiment, (a) is a perspective view and (b) is an exploded perspective view.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings.

[First Embodiment]
1A and 1B are microchannel plates according to a first embodiment of the present invention, in which FIG. 1A is a perspective view and FIG. 1B is an exploded perspective view. As shown in the figure, the microchannel plate 100 according to the present embodiment includes a hard plate member 110 having a concave portion formed on the surface, and a sheet member 120 laminated on the surface of the plate member 110, The reagent and the specimen are mixed in the space in the recess.

  The plate member 110 is made of transparent plastic and has a mirror surface treatment on the front and back surfaces. The plate member 110 has a width of 20.0 mm, a length of 80.0 mm, and a thickness of 1.5 mm. The space in the recess formed in the plate member 110 includes a mixing space 111, a reagent storage space 112, and a reagent channel 116.

  The mixing space 111 is a space in an annular recess formed in the center of the plate member 110 and having a diameter of 7.0 mm and a depth of 0.8 mm. In the mixing space 111, the specimen is accommodated, and the specimen and the reagent accommodated in the reagent accommodating space 112 are mixed. The mixing space 111 may be provided with a trap mechanism such as a double-sided tape for holding the specimen, and an absorption mechanism such as felt or foam for absorbing excess reagent.

  The reagent storage space 112 is a space in an annular recess formed adjacent to the mixing space 111 and having a diameter of 10.0 mm and a depth of 1.0 mm. In the reagent storage space 112, the reagent is stored in a liquid state or sealed in a container such as a blister pack.

  The reagent channel 116 is a space in a recess having a width of 2.0 mm and a depth of 0.4 mm that connects the mixing space 111 and the reagent storage space 112.

  The sheet member 120 is made of an elastomer molded to have a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.8 mm, and the plate member 110 covers the mixing space 111, the reagent storage space 112, and the reagent channel 116. It is laminated on the surface of.

  As described above, in the microchannel plate 100 according to the present embodiment, since the reagent storage space 112 in which the reagent is stored is provided, the reagent can be stored in the reagent storage space 112 in advance. Furthermore, in the microchannel plate 100 according to the present embodiment, since the sheet member 120 is made of an elastomer, the reagent stored in the reagent storage space 112 is removed by pressing a portion covering the reagent storage space 112 of the sheet member 120. It can flow into the mixing space 111. Therefore, according to the microchannel plate 100 according to the present embodiment, it is possible to easily and quickly test the specimen.

[Second Embodiment]
2A and 2B are microchannel plates according to a second embodiment of the present invention, in which FIG. 2A is a perspective view and FIG. 2B is an exploded perspective view. As shown in the figure, the micro-channel plate 200 according to the present embodiment includes a hard plate member 210 having a concave portion formed on the surface, a sheet member 220 laminated on the surface of the plate member 210, and the sheet. The back plate member 230 laminated on the surface of the member 220 is configured so that the reagent and the sample are mixed in the space in the recess as in the microchannel plate 100 according to the first embodiment. .

  The plate member 210 is made of a transparent plastic having a width of 20.0 mm, a length of 80.0 mm, and a thickness of 2.5 mm. As shown in FIGS. 3A and 3C, the space in the recess formed on the surface of the plate member 210 includes a mixing space 211, reagent storage spaces 212 and 213, a pump space 214, and a waste liquid reservoir. A space 215 and a plurality of flow paths 216a to 216f are included. Further, the plate member 210 has an air suction port 217 and a waste liquid discharge port 218 formed with the recess extending to the end. Further, as shown in FIGS. 3B and 3C, a concave portion 219 for observing the specimen using a microscope is formed on the back surface of the plate member 210. In addition, the plate member 210 is subjected to mirror surface treatment on at least the bottom surface of the recesses forming the mixing space 211 and the bottom surface of the recesses 219 formed on the back surface.

  The mixing space 211 and the reagent storage spaces 212 and 213 are the same as those provided in the microchannel plate 100 according to the first embodiment. In the microchannel plate 200 according to this embodiment, two reagents are used. The accommodation spaces 212 and 213 are provided at symmetrical positions around the mixing space 211. In these two reagent storage spaces 212 and 213, the same type or different types of reagents are stored in a liquid state or in a state of being enclosed in a blister pack, respectively.

  The pump space 214 and the waste liquid storage space 215 are spaces in an annular recess having a diameter of 12.0 mm and a depth of 1.8 mm, which are formed symmetrically around the mixing space 211. The portion of the sheet member 220 that covers the pump space 214 is continuously pressed, so that air is sucked and discharged in the pump space 214, and the reagent that has been pushed out by the air and has flowed out of the mixing space 211. Is stored in the waste liquid storage space 215.

  The plurality of channels 216a to 216f include reagent channels 216a and 216b that connect the mixing space 211 and the reagent storage spaces 212 and 213, an air discharge channel 216c that connects the pump space 214 and the mixing space 211, A waste liquid channel 216d that connects the mixing space 211 and the waste liquid storage space 215, an air suction channel 216e that connects the air suction port 217 and the pump space 214, and a waste liquid storage space 215 and a waste liquid discharge port 218 are connected. A waste liquid discharge channel 216f is included. These channels 216a to 216f have a width of 2.0 mm and a depth of 0.4 mm.

  The sheet member 220 is made of an elastomer molded to have a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.8 mm. As shown in FIG. 4, back flow prevention valves 221 a to 221 d having a width of 2.0 mm and a height of 0.4 mm are provided on the back surface of the sheet member 220 in order to prevent the reagent and air from flowing back. . Specifically, a backflow prevention valve 221a for preventing the reagent from flowing back from the mixing space 211 to the reagent storage space 212 is provided at the boundary between the mixing space 211 and the reagent flow path 216a. A backflow prevention valve 221b for preventing the reagent from flowing back from the mixing space 211 to the reagent storage space 213 is provided at the boundary between the mixing space 211 and the reagent flow path 216b. A backflow prevention valve 221c for preventing the reagent from flowing back from the mixing space 211 to the pump space 214 is provided at the boundary between the mixing space 211 and the air discharge channel 216c. A backflow prevention valve 221 d for preventing air in the pump space 214 from being discharged from the air suction port 217 is provided in the vicinity of the air suction port 217.

  As shown in FIG. 5, the backflow prevention valves 221 a to 221 d are protrusions that are inclined in the forward flow direction of the reagent or air, and are integrally formed with the sheet member 220. The positive flow direction is a direction from the reagent storage spaces 212 and 213 through the mixing space 211 to the waste liquid storage space 215 in the case of a reagent, and from the air suction port 217 through the pump space 214 in the case of air. The direction is toward the mixing space 211. As shown in FIG. 5B, the backflow prevention valves 221a to 221d are opened by the flow pressure of the reagent or air when the reagent or air flows in the forward flow direction. On the other hand, as shown in FIG. 5 (a), when the reagent or the like is about to flow in the reverse flow direction, the reagent is closed and the flow of the reagent or air is blocked.

  The back plate member 230 is made of plastic having a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.8 mm. As shown in FIG. 6, the back plate member 230 is formed with a plurality of annular pressing holes 231 to 235 penetrating in the thickness direction. The plurality of pressing holes 231 to 235 can press the upper part of the mixing hole 211 of the sheet member 220 and the upper part of the pressing hole 231 having a diameter of 7.0 mm and the reagent storage spaces 212 and 213 of the sheet member 220. Pressure holes 232 and 233 having a diameter of 10.0 mm, and pressure holes 234 and 235 having a diameter of 12.0 mm that enable pressing above the pump space 214 and the waste liquid storage space 215 of the sheet member 220 are included.

  Further, as shown in FIG. 6A, on the back surface of the back plate member 230, a flow path made of plastic having a width of 4.0 mm and a thickness of 0.2 mm above the flow paths 216a to 216f of the plate member 210. A convex part 236 wider than 216a to 216f is integrally formed. Further, as shown in the figure, the convex portion 236 is also formed on the periphery of the pressing holes 231 to 235. For this reason, the pressure applied to the sheet 220 member is highest in the portion sandwiched between the convex portion 236 and the plate member 210 (peripheral portions such as the flow paths 216a to 216f and the mixing space 211). Therefore, the adhesiveness between the sheet member 220 and the plate member 210, particularly the adhesiveness of the peripheral portions such as the flow paths 216 a to 216 f and the mixing space 211 is improved, and the reagent leaks from between the sheet member 220 and the plate member 210. Can be prevented.

  Next, a method for inspecting a fungus parasitic on human skin (specimen) using the microchannel plate 200 according to the present embodiment will be described.

  First, the mixing space of the microchannel plate 200 in which caustic (reagent) for dissolving the skin is accommodated in the reagent accommodating space 212 and fungiflora Y (reagent) for staining the fungus is accommodated in the reagent accommodating space 213. In 211, the skin collected from the subject is accommodated.

  After the skin is accommodated in the mixing space 211, the plate member 210 and the sheet member 220 are firmly bonded with a double-sided tape, an adhesive, or the like. The sheet member 220 and the back plate member 230 are bonded together in advance.

  Subsequently, in order to mix the skin with caustic and fungiflora Y, the portion of the sheet member 220 covering the reagent containing spaces 212 and 213 is pressed. As a result, the caustic and fungiflora Y stored in the reagent storage spaces 212 and 213 flow into the mixing space 211 through the reagent flow paths 216a and 216b, respectively. Then, the skin housed in the mixed space 211 is mixed with caustic and fungiflora Y, so that a part of the skin is dissolved and the fungus parasitic on the skin is dyed.

  Further, in order to cause waste liquid including caustic and fungiflora Y in the mixing space 211 and dissolved skin and the like to flow out of the mixing space 211, a portion covering the pump space 214 of the sheet member 220 is continuously pressed (FIG. 2). (See arrow 1 in (a)). As a result, the air sucked from the air suction port 217 (see arrow 2 in FIG. 2B) flows into the mixing space 211 through the air suction flow path 216e, the pump space 214, and the air discharge flow path 216c. The waste liquid in the mixing space 211 is pushed out by the air, flows into the waste liquid storage space 215 through the waste liquid flow path 216d, and is discharged from the waste liquid discharge port 218 (see arrow 3 in FIG. 2B). As a result, only the skin stained with Fungiflora Y remains in the mixed space 211.

  And by observing with a microscope from the recessed part 219 formed in the back surface of the plate member 210, it can be confirmed whether the fungus is parasitic on skin.

  The skin accommodated in the mixed space 211 is held by a trap mechanism such as a double-sided tape. However, when the portion covering the mixed space 211 of the sheet member 220 is pressed, the skin is formed between the sheet member 220 and the trap mechanism. Can hold the skin more securely.

  Moreover, it is preferable that a trap mechanism consists of a double-sided tape etc. which show the poor solubility with respect to caustic and are not dye | stained by Fungiflora Y. Similarly, the plate member 210 is preferably made of a plastic that does not contain a benzene ring, such as polyolefin that is not dyed by the Fungiflora Y.

[Third Embodiment]
7A and 7B are microchannel plates according to a third embodiment of the present invention, in which FIG. 7A is a perspective view and FIG. 7B is an exploded perspective view. As shown in the figure, the microchannel plate 300 according to the present embodiment includes a hard first plate member 310 having a recess formed on the surface, and a first sheet laminated on the surface of the first plate member 310. A member 320, a hard second plate member 330 having a recess formed in the front and back surfaces and at least one through-hole penetrating in the thickness direction, laminated on the surface of the first sheet member 320; The second sheet member 340 laminated on the surface of the plate member 330 and the back plate member 350 laminated on the surface of the second sheet member 340, and the first in the recess formed in the first plate member 310. The reagent and the specimen are mixed in a space including the space and the second space in the through hole and the recess formed in the second plate member 330.

  The second plate member 330 is made of a plastic having a width of 20.0 mm, a length of 80.0 mm, and a thickness of 2.5 mm. As shown in FIG. 8, a mixing space 331, a reagent storage space 332, 333, a pump space 334, and an upper waste liquid storage space are provided in the second space in the through hole and in the recess formed in the second plate member 330. 335 and a plurality of flow paths 336a to 336e are included. The second plate member 330 has an air suction port 337 formed with a recess extending to the end.

  As shown in FIG. 8, the plurality of flow paths 336 a to 336 e include reagent flow paths 336 a and 336 b and upstream waste liquid flow paths 336 c made up of spaces in the through holes, air suction flow paths 336 d made up of spaces in the recesses, and And an air discharge channel 336e.

  As shown in FIG. 8C, the reagent flow path 336b (336a) connects the recess 333b (332b) constituting the reagent storage space 333 (332) and the mixing space 331, for example, a through hole having a diameter of 0.5 mm. It is the space in the hole.

  The upstream waste liquid flow path 336c is a space in a through hole that connects the mixing space 331 and the communication hole 321 of the first sheet member 320, and the diameter decreases toward the back side as shown in FIG. 8 (d). And an upper part having a constant diameter (2.0 mm). The upper portion of the upstream waste liquid flow path 336c has a maximum diameter of 1.0 mm and a minimum value of 0.5 mm. On the other hand, as shown in FIG. 9A, a filter 338 that allows the waste liquid to pass through without passing the specimen and a bush 339 for supporting the filter 338 are accommodated in the lower portion of the upstream side waste liquid channel 336c. Has been. As shown in the figure, the bush 339 is supported by the first sheet member 320.

  As shown in FIG. 8A, the air suction channel 336d is a space in a recess having a width of 2.0 mm and a depth of 0.5 mm that connects the air suction port 337 and the pump space 334. The air discharge flow path 336e is a space in a recess having a width of 2.0 mm and a depth of 0.3 mm that connects the pump space 334 and the mixing space 331.

  As shown in FIG. 8, the reagent storage space 333 (332) includes a space in the first through hole 333a (332a) having a diameter of 10.0 mm, the first through hole 333a (332a), and the second through hole 336b ( 336a) and a space in the first recess 333b (332b) formed at the periphery on the back surface side of the first through hole 333a (332a). Further, as shown in FIG. 9B, the reagent storage spaces 332 and 333 store blister packs 360 in which reagents are sealed. The blister pack 360 has a tongue-shaped protrusion 361 formed on the back side, and a spout for pouring out the reagent is formed at the tip of the protrusion 361.

  As shown in FIG. 8A, the mixing space 331 includes a space in the second recess formed on the surface of the second plate member 330. Specifically, the space in the second recess is a depth formed continuously from the space in the annular recess having a diameter of 6.0 mm and a depth of 0.5 mm and a part of the outer periphery of the recess. It consists of a space in a concave portion having a triangular shape in plan view of 0.5 mm and a side length of 2.0 mm. As shown in the figure, in the mixing space 331, reagent flow paths 336a and 336b are connected to the bottom surface of the annular recess, and the upstream waste liquid flow consisting of the space in the third through hole is formed on the bottom surface of the triangular recess. The path 336c is connected.

  The pump space 334 is a space in a through hole having a diameter of 12.0 mm. In the pump space 334, when the lower part of the pump space 334 of the first sheet member 320 and the upper part of the pump space 334 of the second sheet member 340 are continuously pressed, air is sucked and discharged.

  The upper waste liquid storage space 335 is a space in a through hole having a diameter of 12.0 mm. In the upper waste liquid storage space 335, waste liquid containing a reagent and the like flowing out from the mixing space 331 is stored.

  The first sheet member 320 is made of an elastomer molded to have a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.7 mm. As shown in FIG. 10, the first sheet member 320 has a communication hole 321 having a diameter of 0.5 mm that communicates with the upstream waste liquid flow path 336 c of the second plate member 330, and an upper waste liquid reservoir of the second plate member 330. A communication hole 325 having a diameter of 12.0 mm and communicating with the space 335 is formed.

  The first plate member 310 is made of a plastic having a width of 20.0 mm, a length of 80.0 mm, and a thickness of 1.0 mm. As shown in FIG. 11, the first space in the recess formed in the first plate member 310 includes a lower waste liquid storage space 315 and a downstream waste liquid flow path 316. The lower waste liquid storage space 315 communicates with the upper waste liquid storage space 335 of the second plate member 330 and the communication hole 325 of the first sheet member 320, and is a space in an annular recess having a diameter of 12.0 mm and a depth of 0.5 mm. It is. The downstream side waste liquid flow path 316 is a space in a recess having a width of 2.0 mm and a depth of 0.3 mm, one end communicating with the communication hole 321 of the first sheet member 320 and the other end connected to the lower waste liquid storage space 315. It is. Further, the first plate member 310 is formed with a pressing hole 314 having a diameter of 12.0 mm that allows the lower portion of the pump space 334 of the first sheet member 320 to be pressed.

  The second sheet member 340 is made of an elastomer molded to have a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.7 mm. As shown in FIG. 12, an observation hole 341, pressing holes 342 and 343, and a communication hole 345 are formed in the second sheet member 340. The observation hole 341 is a through hole that is provided above the mixing space 331 of the second plate member 330 in order to observe the specimen using a microscope and has the same shape as that of the mixing space 331 in plan view. The pressing holes 342 and 343 are through holes having a diameter of 10.0 mm provided above the reagent storage spaces 332 and 333 of the second plate member 330. The communication hole 345 is a through hole having a diameter of 12.0 mm that communicates with the upper waste liquid storage space 335 of the second plate member 330. In addition, on the back surface of the second sheet member 340, backflow prevention valves 346a and 346b having a width of 2.0 mm and a height of 0.4 mm are provided in order to prevent the reagent and air from flowing back. The backflow prevention valve 346a is provided at the boundary between the mixing space 331 and the air discharge flow path 336e in order to prevent the reagent in the mixing space 331 from flowing back into the pump space 334. The backflow prevention valve 346 b is provided in the vicinity of the air suction port 337 in order to prevent air in the pump space 334 from being discharged from the air suction port 337.

  The back plate member 350 is made of a transparent plastic having a width of 20.0 mm, a length of 80.0 mm, and a thickness of 0.7 mm. As shown in FIG. 13, the back plate member 350 is formed with a plurality of annular pressing holes 352 to 354 and a waste liquid discharge port 355 penetrating in the thickness direction. The pressing holes 352 and 353 are through holes having a diameter of 10.0 mm that communicate with the pressing holes 342 and 343 of the second sheet member 340 and allow the blister pack 360 stored in the reagent storage spaces 332 and 333 to be directly pressed. is there. The pressing hole 354 is a through-hole having a diameter of 12.0 mm that allows pressing above the pump space 334 of the second sheet member 340. The waste liquid discharge hole 355 is a through hole having a diameter of 1.0 mm that communicates with the communication hole 345 of the second sheet member 340.

  Next, a method for inspecting fungi parasitic on human skin (specimen) using the microchannel plate 300 according to the present embodiment will be described.

  First, a blister pack 360a encapsulating caustic is stored in the reagent storage space 332, and a blister pack 360b enclosing Fungiflora Y is collected from the subject in the mixing space 331 of the microchannel plate 300 stored in the reagent storage space 333. Accommodate skin.

  After the skin is accommodated in the mixing space 331, the second sheet member 340 and the back plate member 350 are firmly bonded with a double-sided tape, an adhesive, or the like. The first plate member 310 and the first sheet member 320, the first sheet member 320 and the second plate member 330, and the second plate member 330 and the second sheet member 340 are bonded together in advance.

  Subsequently, in order to mix the skin with caustic and fungiflora Y, the blister packs 360a and 360b are directly pressed from the pressing holes 352 and 353 of the back plate member 350 and the pressing holes 342 and 343 of the second sheet member 340. As a result, caustic and fungiflora Y sealed in the blister packs 360a and 360b flow into the mixing space 331 through the reagent flow paths 316a and 316b, respectively. Then, the skin housed in the mixing space 331 is mixed with caustic and fungiflora Y, so that a part of the skin is dissolved and the fungus parasitic on the skin is dyed.

  Further, in order to move the stained skin of the mixing space 331 to the surface of the filter 338, the lower portion of the pump space 334 of the first sheet member 320 and the upper portion of the pump space 334 of the second sheet member 340 are continuously pressed. (See arrow 1 in FIG. 7A). As a result, the air sucked from the air suction port 337 (see arrow 2 in FIG. 7B) flows into the mixing space 331 through the air suction flow path 336d, the pump space 334, and the air discharge flow path 336e. The stained skin in the mixing space 211 and waste liquid such as caustic and fungiflora Y are pushed out by the air to the upstream waste liquid flow path 336c. The stained skin moves to the surface of the filter 338 provided in the upstream side waste liquid flow path 336c. On the other hand, after passing through the filter 338, the waste liquid flows into the lower waste liquid storage space 315 through the communication hole 321 and the downstream side waste liquid flow path 316, and is discharged from the waste liquid discharge port 355 (arrow in FIG. 7A). 3).

  Then, by observing the surface of the filter 338 with a microscope from the upper part of the observation hole 341 formed in the second sheet member 340 of the back plate member 350, it can be confirmed whether or not fungus is parasitic on the skin.

[Fourth Embodiment]
14A and 14B are microchannel plates according to a fourth embodiment of the present invention, in which FIG. 14A is a perspective view and FIG. 14B is an exploded perspective view. As shown in the figure, the micro flow path plate 400 according to the present embodiment has a micro flow plate according to the first embodiment except that the plate member 410 is made of an elastomer and the sheet member 420 is made of a transparent plastic. It is the same as the road plate 100.

  Further, in the space in the recess formed in the plate member 410, the mixing space 411, the reagent storage space 412, the mixing space 411 and the reagent storage space are the same as in the microchannel plate 100 according to the first embodiment. A reagent flow path 416 that connects 412 is included. The mixing space 111 is provided with a trap mechanism for holding the specimen. The trap mechanism is a double-sided tape that does not react with the reagent.

  As described above, in the microchannel plate 400 according to the present embodiment, since the plate member 410 is made of an elastomer, the sample can be easily collected by bending the plate member 410 and rubbing the trap mechanism against the sample. . Furthermore, in the microchannel plate 400 according to the present embodiment, the reagent stored in the reagent storage space 412 can be caused to flow into the mixing space 411 by pressing the lower portion of the plate member 410 reagent storage space 412. Therefore, according to the microchannel plate 400 according to the present embodiment, it is possible to easily and quickly test the specimen.

  Although preferred embodiments of the present invention have been described above, the present invention is not limited to these configurations.

  For example, in Embodiments 2 and 3 described above, an example in which skin (fungus) is used as a specimen and Kasaikari and Fungiflora Y are used as reagents has been described. However, an antibody in blood is used as a specimen, and an antigen and a labeled antibody are used. By using it as a reagent, various diseases can be examined. In this case, the number and position of the mixing space and the reagent storage space can be arbitrarily changed according to the specimen and the reagent.

  In the first and second embodiments, the trapping mechanism for holding the specimen is provided in the mixing spaces 111 and 211. However, the trapping mechanism is provided in a portion covering the mixing spaces 111 and 211 of the sheet members 120 and 220. It may be provided.

  In the second and third embodiments, the pump spaces 214 and 334 are provided between the air suction passages 216e and 336d and the air discharge passages 216c and 336e, but the air suction passages 216e and 336d and the air discharge passages are provided. The flow paths 216c and 336e may be directly connected, and air may be introduced from the outside using a pump, a syringe, or the like.

  In the second embodiment, the waste liquid storage space 215 is provided between the waste liquid flow path 216d and the waste liquid discharge flow path 217f. However, the waste liquid flow path 216d and the waste liquid discharge flow path 217f are directly connected, and the waste liquid is discharged. The liquid may be discharged from the waste liquid discharge port 218 without being stored in the micro flow path plate. Similarly, in the third embodiment, the lower waste liquid storage space 315 is provided in the first plate member 310 and the lower waste liquid storage space 335 is provided in the second plate member 330. However, the downstream waste liquid flow path 316 is provided in the second plate. You may extend to the edge part of the member 330, and may discharge a waste liquid from there. In this case, the upper waste liquid storage space 315, the communication holes 325 and 345, the upper waste liquid storage space 335, and the waste liquid discharge port 355 need not be provided.

  In addition, it is of course possible to make various design changes and modifications within the scope of the claims attached to this specification.

100, 200, 300, 400 Microchannel plate 110, 210, 410 Plate member 310 First plate member 330 Second plate member 120, 220, 420 Sheet member 320 First sheet member 340 Second sheet member 230, 350 Back plate Member 111, 211, 331, 411 Mixing space 112, 212, 213, 332, 333, 412 Reagent storage space 214, 334 Pump space 215 Waste liquid storage space 315 Lower waste liquid storage space 335 Upper waste liquid storage space 217, 337 Air inlet 218 355 Waste liquid discharge port 231 to 235, 314, 342, 343, 352, 353

Claims (7)

A hard first plate member having a recess formed on the surface, a first sheet member laminated on the surface of the first plate member, and a layer that penetrates in the thickness direction laminated on the surface of the first sheet member. A hard second plate member having at least one through hole and a recess formed on the front and back surfaces; and a second sheet member laminated on the surface of the second plate member, and formed on the first plate member. A microchannel plate in which a reagent and a sample are mixed in a space including a first space in a recess and a second space in a through hole and in a recess formed in the second plate member,
The first sheet member has a communication hole that communicates the first space and the second space;
The second space includes a reagent storage space in which a reagent container filled with the liquid reagent is stored, a mixing space in which the sample is stored and the sample and the reagent are mixed, and the reagent storage A reagent flow path connecting the space and the mixing space, and an upstream waste liquid flow path connecting the mixing space and the communication hole,
The first space includes a waste liquid storage space in which waste liquid containing the reagent that has flowed out of the mixing space through the upstream side waste liquid flow path and the communication hole is stored, and the communication hole and the waste liquid storage space. Including a downstream waste liquid flow path to be connected,
The microchannel plate, wherein the second sheet member has a pressing hole that allows the reagent container accommodated in the reagent accommodating space to be directly pressed. The through hole and the recess formed in the second plate member are a first through hole penetrating in the thickness direction and a first hole formed on the back surface of the second plate member so as to communicate with the first through hole. A recess, a second recess formed on the surface of the second plate member, a second through hole connecting the first recess and the second recess, and the second recess and the first sheet member. A third through hole connecting the communication hole,
The reagent storage space consists of a space in the first through hole and in the first recess,
The mixing space consists of a space in the second recess,
The reagent channel is composed of a space in the second through hole,
The micro flow path plate according to claim 1, wherein the upstream waste liquid flow path includes a space in the third through hole. The second plate member has an air suction port formed by extending a concave portion on the surface of the second plate member to the end,
The second space includes a space in a through hole formed in the second plate member, and includes a pump space in which air is sucked and discharged, and a space in a recess formed on the surface of the second plate member. An air suction flow path that connects the air suction port and the pump space, and an air discharge flow path that connects the pump space and the mixing space, the space being in a recess formed on the surface of the second plate member. And further including
The first plate member has a pressing hole that allows the pump space lower part of the first sheet member to be pressed,
A portion of the second sheet member that covers the air suction flow path is provided with a backflow prevention valve that prevents air from being discharged from the pump space to the air suction port.
When the pump space lower part of the first sheet member and the pump space upper part of the second sheet member are continuously pressed, the air sucked from the air suction port becomes the air suction channel and the pump space. And the waste liquid in the mixing space is pushed out by the air and passes through the upstream waste liquid flow path and the downstream waste liquid flow path. The microchannel plate according to claim 1 or 2, wherein the microchannel plate flows into the reservoir space. A hard back plate member laminated on the surface of the second sheet member;
The back plate member communicates with the pressing hole of the second sheet member and allows the reagent container accommodated in the reagent accommodating space to be directly pressed, and the pump of the second sheet member. The microchannel plate according to claim 3, further comprising a second pressing hole that enables pressing of the upper portion of the space. The back plate member is transparent,
The microchannel plate according to claim 4, wherein the second sheet member has an observation hole for observing the specimen in an upper portion of the mixing space.   The microchannel plate according to any one of claims 1 to 5, wherein the upstream waste liquid flow path is provided with a filter that allows the waste liquid to pass through without passing the specimen.   The microchannel plate according to claim 1, wherein the reagent container is a blister pack.

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