JP2013018731A - Agent for improving brain function and food and drink for improving brain function - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a pharmaceutical product and/or food and drink for improving brain functions, each of which effectively improves memory and learning abilities that degrade with age, effectively improves cranial nerve disease such as dementia represented by Alzheimer disease, and can be safely and easily ingested by a human on a daily basis.SOLUTION: Each of the agent and/or food and drink for improving brain functions contains, as active ingredients, a water extract of Chinese parsley by 0.1 to 5 mass%, and includes transglycosylation rutin and/or transglycosylation hesperidin by 0.1 to 10 mass% in total.

Description

  The present invention relates to a pharmaceutical or food / drink product for improving brain function that can be safely and routinely ingested by humans.

  With the advent of an aging society, the desire to be healthy both physically and mentally is increasing. As humans grow older, their learning and memory skills gradually decline. Apart from this natural providence, dementia such as Alzheimer's disease and Parkinson's disease can lead to serious situations that threaten even the dignity of human beings. In recent years, it has been energetically advanced. For example, as a therapeutic agent for Alzheimer's disease, a brain function improving agent containing bovine phosphatidylcholine or bovine phosphatidylserine (Patent Literature 1), a therapeutic agent containing a salt of deferoxamine as an active ingredient (Patent Literature 2), a docosahexaenoic acid derivative Examples thereof include a brain function improving composition containing an ester, amide, triglyceride, phospholipid derivative or the like as an active ingredient (Patent Document 3).

  However, apart from the effects of the above drugs, the active ingredients of these drugs are not generally distributed as food materials and are not rich in food experience. (Continuously) It is hard to say that it can be ingested easily.

  Under such circumstances, pharmaceuticals and foods and drinks for improving brain function that have the action of effectively improving human brain function and that can be safely and daily consumed by humans are available in the industry. There was a strong demand.

  On the other hand, rutin and hesperidin are plant-derived flavonoids and their physiological functions have been clarified. Furthermore, glycosylated rutin and / or glycosylated hesperidin with increased water solubility are also disclosed, but as an effect of these administration, learning ability and memory ability, or relation to dementia such as Alzheimer's disease and Parkinson's disease There is no disclosure of sex.

Japanese Patent Laid-Open No. 07-017855 JP 58-012123 A Japanese Patent Laid-Open No. 02-049723

  The present invention effectively improves memory / learning ability associated with aging and effectively improves cranial nerve diseases such as dementia typified by Alzheimer's disease. It aims at providing the pharmaceutical and food-drinks for brain function improvement which can be performed.

  In order to achieve the above-mentioned object, the present inventors, as an active ingredient, as a result of earnest research on ingredients that can effectively improve memory and learning ability that occurs with aging from familiar food materials, Discovered that a brain function improving agent or food / beverage product for improving brain function comprising a Chinese parsley water extract and sugar-transferred rutin and / or sugar-transferred hesperidin at a predetermined ratio solves the above-mentioned problems and completed the present invention. It came to do.

That is, the embodiment of the present invention is as follows.
(1) A liquid pharmaceutical comprising 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient and 0.1 to 10% by mass in total of transglycosyl rutin and / or transglycosyl hesperidin Brain function improving agent.
(2) Contains 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient, 0.1 to 10% by mass in total of transglycosyl rutin and / or transglycosyl hesperidin, and has a pH of 3 to 5 brain function improving agents (for example, liquid pharmaceuticals).
(3) The brain function improving agent according to (1) or (2), further comprising at least 0.01% by mass (preferably 0.01 to 1% by mass) of a coffee extract.
(4) Brain function-improving food or drink containing 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient and 0.1 to 10% by mass in total of transglycosyl rutin and / or transglycosyl hesperidin Goods.
(5) As an active ingredient, 0.1 to 5% by mass of a Chinese parsley water extract, 0.1 to 10% by mass in total of transglycosyl rutin and / or transglycosyl hesperidin, and having a pH of Food or drink for improving brain function, which is 3 to 5.
(6) The food or drink for improving brain function according to (4) or (5), further comprising at least 0.01% by mass (preferably 0.01 to 1% by mass) of coffee extract .

  ADVANTAGE OF THE INVENTION According to this invention, when the human body takes this invention goods on a daily basis (continuous), the fall of memory and learning ability which arises with an aging can be improved effectively. The product of the present invention can effectively improve cranial nerve diseases such as dementia typified by Alzheimer's disease. From the fact that both the sugar-transferred rutin and / or sugar-transferred hesperidin, or the Chinese parsley water extract and the coffee extract, which are the active ingredients of the product of the present invention, have been used as food ingredients for a long time. The product of the present invention containing these components as an active ingredient has an advantage that humans can safely and easily take it on a daily basis.

  In the present invention, improvement of brain function means improvement of a decrease in memory / learning ability caused by aging, and improvement of symptoms such as Alzheimer's disease, which is a typical disease of dementia.

  Chinese parsley (English name: coriander), which is an active ingredient of the product of the present invention, is a plant belonging to the genus Ciendro, and in Europe, its leaf part is sometimes called Cilantro. On the other hand, Chinese parsley is sometimes called a cilantro in Japan, regardless of the part of the leaf or dried grain. The Chinese parsley water extract used in the present invention means a product obtained by extracting water from a whole plant of Chinese parsley. That is, the Chinese parsley water extract used in the present invention means a component that is substantially extracted with water among components contained in Chinese parsley.

  As a specific extraction method, for example, after removing roots from whole grass of Chinese parsley, draining, and then mincing with a blender, and passing this through a filter using a centrifuge Filter. The obtained filtrate is heat-treated at 121 ° C. for 10 minutes and allowed to cool to room temperature to obtain a Chinese parsley water extract used in the present invention. The Chinese parsley water extract thus obtained usually contains about 500 to 20,000 μg / ml of polyphenol. Although the solids concentration of the Chinese parsley water extract varies depending on the temperature and time when the Chinese parsley is heated, the amount of Chinese parsley contained in the product of the present invention is included in the above Chinese parsley water extract. Based on polyphenol content. That is, in many cases, the product of the present invention contains other components other than polyphenol contained in the Chinese parsley water extract together with polyphenol. The amount of the Chinese parsley water extract contained in the product of the present invention It means the polyphenol content contained in the water extract.

  The preferred content of the Chinese parsley water extract in the product of the present invention is 0.1 to 10% by mass, preferably 0.1 to 5% by mass, and more preferably 0.5 to 3% by mass. When a Chinese parsley water extract is used in combination with sugar-transferred rutin and / or sugar-transferred hesperidin, which will be described later in the above range, the desired effect of the present invention is effectively or synergistically increased. On the other hand, when the concentration of the Chinese parsley water extract is out of the above lower limit or upper limit, even if it is used in combination with glycosylated rutin and / or glycosylated hesperidin, sufficient effects due to the combined use cannot be obtained.

  The transglycosyl rutin, which is an active ingredient of the product of the present invention, is an equimolar or more (usually 1 to 20) D-glucose molecule bound by α-1,4 bond at the 4-position of D-glucose in rutin. It means α-glycosyl rutin or a mixture of various α-glycosyl rutins having different molecular numbers of D-glucose. Glucose transfer rutin is a highly safe substance that has been used in the fields of food, cosmetics, pharmaceuticals, etc., and is finally hydrolyzed into rutin and D-glucose by the action of enzymes in the body. It is degraded and exerts its original physiological effects. Examples of the physiological action of rutin include capillary strengthening action, bleeding prevention action, blood pressure regulation action and the like.

  The transglycosylated hesperidin, which is an active ingredient of the product of the present invention, is an equimolar or more (usually 1 to 20) D-glucose molecule bonded with α-1,4 bond at the 4-position of D-glucose in hesperidin. It means α-glycosyl hesperidin or a mixture of various α-glycosyl hesperidins having different numbers of D-glucose molecules. Glycosylated hesperidin is a highly safe substance conventionally used in the fields of foods, cosmetics, pharmaceuticals, etc., and is finally hydrolyzed into hesperidin and D-glucose under the action of enzymes in the body. It is degraded and exerts the original physiological effects of hesperidin. Examples of the physiological action of hesperidin include capillary strengthening action, bleeding prevention action, blood pressure regulation action and the like.

  The above-mentioned glycosyl rutin and glycosyl hesperidin can be prepared by various methods, but from the viewpoint of economy, a biochemical method using a glycosyltransferase is advantageous. Examples of the method include rutin or hesperidin, α-glucosidase, cyclomaltodextrin / glucanotransferase, and α-amylase in the presence of an α-glucosyl sugar compound such as a partially hydrolyzed starch or maltooligosaccharide. Can be obtained in a high yield by allowing a plurality of types of enzymes to act. Regarding such enzyme reaction, JP-A-3-7593, JP-A-3-27293, JP-A-3-58790, JP-A-3-115292, JP-A-10-70994, Japanese Patent Application No. A method described in Japanese Patent Laid-Open No. 9-69588 can be exemplified. Commercially-available products prepared by such a method include sugar-transferred rutin powder (trade name “αG rutin P” (total rutin content 40 to 44% per solid weight) or trade name “αG rutin PS” (solid (All rutin content per minute weight 80 to 84%), all Toyo Seika Co., Ltd.) and sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content 70% or more per solid weight), Hayashibara Co., Ltd. Trading). Although depending on the method of use, in the present invention, the sugar-transferred rutin and the sugar-transferred hesperidin do not necessarily have to be purified to the highest purity, and their preparation methods are not required unless they significantly disturb the intended purpose. The thing of the form containing the contaminant originating in may be sufficient.

  The preferred content of transglycosyl rutin and / or transglycosyl hesperidin contained in the product of the present invention is 0.1 to 10% by mass in total, more preferably 0.5 to 5% by mass, and still more preferably. Is 0.8 to 5% by mass. Moreover, when the total amount of glycosylated rutin and / or glycosylated hesperidin deviates from the above lower limit and / or upper limit, it is not preferable because the effects achieved by the present invention are significantly reduced or not exhibited. The amount of transglycosyl rutin and / or transglycosyl hesperidin contained in the product of the present invention means the total rutin and / or total hesperidin contained in the sample.

  The production method of the product of the present invention is not limited as long as it contains Chinese parsley water extract, sugar-transferred rutin and / or sugar-transferred hesperidin in the above range as active ingredients. For example, a predetermined amount of water and a Chinese parsley water extract as an active ingredient, sugar-transferred rutin and / or sugar-transferred hesperidin are uniformly mixed, and the active ingredient is prepared so as to be included in the predetermined range. After sterilizing this by one or more sterilization methods selected from known microfiltration, ultraviolet sterilization, heat sterilization, radiation sterilization, autoclave, and autoclave sterilization, After aseptically filling a container of the material, shape and capacity of the above, or filling an appropriate container with a liquid before sterilization, one or more of the above sterilization methods may be appropriately applied and sterilized. is there. As the container, a transparent or light-shielding glass container, a metal container, a paper container, or a polyethylene container, a polyethylene terephthalate container, and the like, which do not substantially change the quality during the storage period, and Examples thereof include plastic containers such as polypropylene containers and pouch containers. Although there is no restriction | limiting in the capacity | capacitance of these containers, For example, containers, such as 1 ml or more, 50 ml or more, 0.1 to 2L, 5 to 25L, or 100 to 2,000L, can be selected suitably and can be used. Furthermore, when a large amount of the product of the present invention is transported, it is optional to fill and transport the container, tank lorry, tanker, etc. of an appropriate volume.

  The form of the product of the present invention means not only liquids as described above, but also general drugs, quasi-drugs, foods and drinks (including beverages) that can be taken orally by humans, such as gels and pastes. To do. The product of the present invention is adjusted to an acidic pH, preferably pH 3 to 5, more preferably pH 3 to 4.5, and still more preferably pH 3.5 to 4, particularly in a liquid state. It is desirable that The pH of the product of the present invention is adjusted to fall within the above range using an appropriate pH adjuster. Any pH adjusting agent can be used as long as it is recognized as a food additive. However, in the present invention, when organic acids such as acetic acid and citric acid and salts thereof are used as a pH adjuster, there is an advantage that the intended effects of the present invention are more remarkably exhibited. When the pH is lower than the lower limit of the above range or higher than the upper limit, the intended effect of the present invention is remarkably reduced or not exhibited, and the storage stability of the active ingredient is reduced or exhibited. This is not preferable because the taste is significantly reduced.

  All of the active ingredients of the product of the present invention are highly safe and can be safely ingested by humans, and all of the above ingredients are industrially stable and inexpensively supplied. Therefore, the product of the present invention has an advantage that it is industrially inexpensive and stably provided.

  And to the product of the present invention, alcohol, starch, protein, amino acid, fiber, sugar (glucose, maltose, trehalose, etc.), sugar alcohol (sorbitol, maltitol, etc.) as long as the desired effect is not hindered. ), Fatty acids including lipids, essential fatty acids, vitamins (L-ascorbic acid, 2-O-α-D-glucosyl-L-ascorbic acid, etc.), minerals (magnesium, calcium, zinc, potassium, iron) , Sodium and their salts, etc.), herbal medicine, flavoring, coloring, sweetener, seasoning (including high-intensity sweeteners such as sucralose, saccharin, stevia, aspartame), stabilizers, preservatives, etc. 1 type, or 2 or more types of the raw material and / or raw material which are normally used can be mix | blended. In addition, pharmaceuticals and pharmaceuticals such as additives, stabilizers, surfactants, preservatives (antibacterial agents), thickeners, antioxidants, chelating agents, aqueous media, water-soluble polymers, pH adjusters, foaming agents, etc. It can also be set as the pharmaceutical formulation which mix | blended combining suitably 1 type, or 2 or more types of the pharmacologically acceptable component in quasi-drug preparation manufacture. Then, in consideration of the composition of the target food composition or preparation and the purpose of use, the active ingredient may be blended in the process from the raw material stage to the completion of the product.

  The product of the present invention can effectively improve the memory / learning ability associated with aging by daily oral intake by humans. The dose is usually 5 to 1,000 mL / adult per adult. Daily, preferably 25 to 500 mL / day, more preferably 50 to 500 mL / day in an amount of 1 to 10 times / day, preferably 3 to 6 times / day at intervals of every day or several days Ingestion for 1 week or longer, preferably 1 month or longer, more preferably 6 months or longer.

  Examples of the application target of the present invention include humans who want to suppress or improve the decrease in memory / learning ability associated with aging, or humans who develop or are at risk of developing Alzheimer's. In addition, the beverage for improving brain function of the present invention can not only suppress or improve a decrease in memory / learning ability associated with aging, but also exhibits an excellent effect for maintaining and promoting health.

  Examples of the present invention will be described below, but the present invention is not limited to these examples, and various modifications can be made within the technical idea of the present invention.

(Manufacture of beverages for improving brain function)
The product of the present invention and comparative products were produced as follows.

  2 parts by mass of sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content of 70% or more by weight of solid content), Hayashibara Corporation) and 10 parts by mass of purified water are stirred and mixed uniformly. Was adjusted to pH 3.8 and finely filtered to obtain a beverage (Reference Example 1).

  Glucose transfer rutin powder (trade name “αG rutin PS” (total rutin content per solid content weight 80 to 84%), Toyo Seika Co., Ltd.) 0.8 parts by mass and 10 parts by mass of purified water are stirred and mixed uniformly. Then, the pH was adjusted to 3.8 with sodium acetate, followed by microfiltration to obtain a beverage (Reference Example 2).

  Roots were removed from the whole harvested Chinese parsley, washed with water, drained, and then minced with a blender. The shredded Chinese parsley was filtered using a 150 mesh filter using a centrifugal filter separator (number of revolutions 3,000 rpm). The filtrate was collected, treated at 121 ° C. for 10 minutes, and allowed to cool to 25 ° C. to obtain a Chinese parsley water extract (Reference Example 3). The polyphenol content in the extract was about 1,000 μg / ml.

  0.3 parts by mass of Chinese parsley water extract obtained in Reference Example 3, sugar-transferred rutin powder (trade name “αG rutin PS” (total rutin content 80 to 84% per solid weight), Toyo Seika Co., Ltd.) 1 Part by mass and 10 parts by mass of purified water were uniformly stirred and mixed, adjusted to pH 3.8 with sodium acetate, and subjected to microfiltration to obtain a beverage preparation according to the present invention (Test Example 1).

  0.3 parts by mass of Chinese parsley water extract obtained in Reference Example 3, sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content of 70% or more per solid weight), Hayashibara Corporation) 2 parts by mass and 10 parts by mass of purified water were stirred and mixed uniformly, adjusted to pH 3.8 with sodium acetate, and subjected to microfiltration to obtain a beverage preparation according to the present invention (Test Example 2).

  2 parts by weight of Chinese parsley water extract obtained in Reference Example 3, sugar-transferred rutin powder (trade name “αG rutin PS” (total rutin content 80 to 84% per solid weight), 2 parts by weight of Toyo Seika Co., Ltd.) , Sugar transfer vitamin C powder (trade name “Asco Fresh”, Hayashibara Shoji Co., Ltd.) 0.2 parts by weight, sodium acetate 0.05 parts by weight, citric acid 2 parts by weight, coffee extract 0.01 parts by weight, and purification 75 parts by mass of water was uniformly stirred and mixed, adjusted to pH 3.7 with acetic acid, and subjected to microfiltration to obtain a beverage that is the product of the present invention (Test Example 3).

  1. 0.5 parts by mass of Chinese parsley water extract obtained in Reference Example 3, sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content of 70% or more per solid weight), Hayashibara Corporation) 5 parts by mass, sugar-transferred vitamin C powder (trade name “Asco Fresh”, Hayashibara Shoji Co., Ltd.) 0.1 part by mass, sodium acetate 0.05 part by mass, citric acid 2 parts by mass, coffee extract 0.01 part by mass And 75 parts by mass of purified water were stirred and mixed uniformly, adjusted to pH 3.5 with sodium acetate, and subjected to microfiltration to obtain a beverage preparation according to the present invention (Test Example 4).

  0.3 parts by mass of Chinese parsley water extract obtained in Reference Example 3, sugar-transferred rutin powder (trade name “αG rutin PS” (total rutin content 80 to 84% per solid weight), Toyo Seika Co., Ltd.) 1 1 part by mass, sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content of 70% or more by weight of solid content, Hayashibara Corporation) and 10 parts by mass of purified water are stirred and mixed uniformly. The pH was adjusted to 3.8 with sodium acetate, and the solution was microfiltered to obtain a beverage that is the product of the present invention (Test Example 5).

  The products of the present invention shown in Test Examples 1 to 5 effectively improve the decline in memory / learning ability that occurs with aging by daily (continuous) ingestion by humans. Can do. In addition, these beverages can effectively improve cranial nerve diseases such as dementia typified by Alzheimer's disease.

(Effectiveness confirmation test I for beverages for improving brain function I)
The following tests were conducted on the beverages obtained in Example 1 in order to compare and confirm the brain function improvement effect in rats.

  As old rats, 20 Wistar male rats (80-week-old) were randomly divided into 4 groups of groups A to D, each of which was divided into 5 groups using the standard feed and drinking water shown in Table 1 below. Bred in the same environment.

  After rats A to D were bred for 10 weeks, a water maze test for learning and memory ability was performed using the Morris water maze (J. Neurosci. Meth., 11, 47-60, 1984). . That is, a circular platform (diameter 20 cm, height 30 cm) was placed in a circular pool (diameter 180 cm, depth 70 cm), and water was poured up to 2 cm above the platform so that the surface of the platform was hidden. Each rat was allowed to swim from a different start position each time, and the time until the rat stopped on the platform for at least 5 seconds (Escape Latency: EL) was measured. The EL measurement time was set to a maximum of 80 seconds from the viewpoint of animal welfare. This test was conducted once every three days once a day for a total of seven times over a three week period. As a test result, the ratio (%) of the rats that have risen to the platform within 60 seconds is shown in Table 2 below, and the time until the rat stops on the platform for 5 seconds or more is shown in Table 3 below.

  In the water maze test, the rate at which all rats in each group reached the platform within 60 seconds increased as the test was repeated. Moreover, since the time for which the rat stays on the platform for 5 seconds or longer has been shortened as the test is repeated, it has been learned and memorized that each rat in each test group can escape from the water by going up to the platform. To be judged. The proportion of rats that reached the platform within 60 seconds was the highest in the D group rats among the A to D group rats, and conversely the lowest in the A group rats. The rats in Group B and Group C were equivalent. On the other hand, the time until the rats stopped on the platform for 5 seconds or longer was the shortest among the rats in the groups A to D, and the longest the rats in the group A. The mice in Group B and Group C were equivalent.

  From this test result, it was found that the brain function improving beverage (Group D) has an action of effectively improving learning / memory ability.

(Effect confirmation test II for beverages for improving brain function, etc.)
The following tests were conducted on the beverages obtained in Example 1 in order to compare and confirm the brain function improvement effect in humans.

  Thirty-five elderly persons (15 males and 20 females) who are physically healthy 60 to 70 years old were subjects, and after daily breakfast, the product of the present invention shown in Test Example 2 was served at a rate of 50 mL / day for 12 weeks. The subjects were allowed to drink, and the memory ability of each subject was evaluated at 6 weeks after the start of drinking and 1 week after the final drinking at 12 weeks, respectively. The evaluation method used was the MAC method (Neurology, Vol. 41, pages 644 to 649, 1991) devised by T. Crook and others. The MAC method is a method for examining and evaluating memory ability in daily life, such as storing a person's face and name, storing a telephone number, and storing a shopping list. The memory age of all the subjects was calculated using the MAC method, and the average value was obtained. The result was 66.5 years old at the start of the test, but 64.2 weeks after the start of drinking. He became 62.8 years old in the first week after the last drink in the 12th week.

  In addition, after the end of this experiment, for all the subjects, after having allowed them to live a normal life for one month without drinking the beverage of the present invention, the memory age of all the subjects was calculated using the MAC method, and the average When the value was calculated, it became 65.5 years old.

  These results indicate that the product of the present invention has an improvement effect on memory ability, and the improvement effect is further enhanced by continuously taking it.

(Effect confirmation test III for beverages for improving brain function III)
The following tests were carried out on the beverage obtained in Example 1 in order to continuously confirm the effect of improving brain function in humans.

  The brains shown in Test Example 2 were 10 healthy subjects aged 50 to 60 who were suspected of having Alzheimer-type dementia and were not administered a dementia treatment drug (5 men and women). The function improving beverage was drunk for 24 weeks at a rate of 100 ml / day after breakfast every day. In addition, confirmation of drinking was performed by a person other than the subject. A dementia diagnostic test was performed on each subject 25 weeks after the start of the drinking test. The test followed the Hasegawa simplified intelligence evaluation scale. The Hasegawa-style simplified intelligence evaluation scale is a test used to determine the symptoms of memory, memorization, and disorientation by asking age and location, or by memorizing simple words. On the Hasegawa simplified intelligence evaluation scale, the higher the score, the lower the suspicion of dementia. The average score of subjects before and after the administration test of the beverage for improving brain function of the present invention was 19 points before the administration test (full score is 30 points, 20 points or less are suspected of dementia), The score was 23 after the administration test. From the results of this study, the subjects' memory, memory, and disorientation symptoms were clearly improved after the study compared to before the administration study.

  In addition, after the completion of this test, the above-mentioned Hasegawa simplified intelligence evaluation scale was performed again for all the subjects, after having had a normal life for one month without drinking the product of the present invention. 20 points.

These results indicate that the product of the present invention has an effect of improving the symptom of memory ability, memorizing ability, and disorientation, and can be effectively produced by continuously taking it.
From this result, the product of the present invention effectively improves memory / learning ability associated with aging and can effectively treat cranial nerve diseases such as dementia typified by Alzheimer's disease. It can be improved.

(Safety confirmation test for beverages for improving brain function)
The following tests were conducted on the beverages of the present invention in order to confirm their safety with mice.

  Chinese parsley water extract obtained by the method of Reference Example 3 and sugar transfer rutin as sugar transfer rutin powder (trade name “αG rutin PS” (total rutin content 80 to 84% per solid weight), Toyo Seika Co., Ltd. ) And sugar-transferred hesperidin, sugar-transferred hesperidin powder (trade name “Hayashibara Hesperidin S” (total hesperidin content of 70% or more by weight of solid content), Hayashibara Corporation) was sterilized according to a conventional method. Each of these sterilized products was injected into the abdominal cavity of ddy mice (10 mice / group) weighing 20 to 25 g, or orally with a gastric sonde, and the course was observed over 7 days. As a result, “Chinese parsley water extract”, “Hayashibara Hesperidin S”, or “αG rutin PS” can be used in any of the administration routes, even in the case of about 10 g administration per kg body weight of the mouse. No deaths were observed.

  From the results of this test, Chinese parsley water extract, sugar-transferred rutin, and sugar-transferred hesperidin, which are active ingredients of the beverage for improving brain function of the present invention, are all safe substances that can be administered to humans.

  The results of Examples 2 to 5 show that the product of the present invention effectively improves the memory / learning ability associated with aging by drinking it on a daily basis, as well as dementia such as Alzheimer's disease. It supports that it can effectively improve cranial nerve diseases and is safe to administer to humans.

  The present invention is summarized as follows.

  The present invention effectively improves memory / learning ability associated with aging and effectively improves cranial nerve diseases such as dementia typified by Alzheimer's disease. The purpose is to provide a drug for improving brain function and / or food and drink.

  And a brain function improving agent comprising 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient, and 0.1 to 10% by mass in total of sugar-transferred rutin and / or sugar-transferred hesperidin and / or Or the said subject is solved with food-drinks.

Claims (6)

  A brain function improving agent comprising 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient, and 0.1 to 10% by mass in total of transglycosyl rutin and / or transglycosyl hesperidin.   As an active ingredient, 0.1 to 5% by mass of Chinese parsley water extract, 0.1 to 10% by mass in total of sugar-transferred rutin and / or sugar-transferred hesperidin, and pH of 3 to 5 Is a brain function improving agent.   The brain function improving agent according to claim 1 or 2, further comprising a coffee extract.   A food and drink for improving brain function comprising 0.1 to 5% by mass of Chinese parsley water extract as an active ingredient, and 0.1 to 10% by mass in total of sugar-transferred rutin and / or sugar-transferred hesperidin.   As an active ingredient, 0.1 to 5% by mass of Chinese parsley water extract, 0.1 to 10% by mass in total of sugar-transferred rutin and / or sugar-transferred hesperidin, and pH of 3 to 5 Food and drink for improving brain function.   The food or drink for improving brain function according to claim 4 or 5, further comprising a coffee extract.