JP2013000047A - On-site article-authenticating system using dna ink - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve the problems that the genuineness of commodity, property or literary work cannot be determined promptly on the site by a conventional mechanism for determining the genuineness of the same.SOLUTION: The on-site article-authenticating system using a DNA ink includes the DNA ink (2) containing a fixed nucleic acid (1), and a detecting nucleic acid (3) binding only to the fixed nucleic acid (1). The indication by printing or marking is carried out by using the DNA ink (2) on the article (6). When determining the genuineness of the article (6), the detecting nucleic acid (3) is spread on the fixed nucleic acid (1) on the site. The genuineness of the article is determined by the presence or absence of a signal because the signal is sent out when the detecting nucleic acid (3) binds with the fixed nucleic acid (1), but the signal is not sent out unless binding.

Description

  The present invention relates to an on-site product authentication system using DNA ink, and printing is performed on an article (including all tangible objects to be transferred; the same shall apply hereinafter) using ink containing a nucleic acid substance having a DNA base sequence such as an orthonormal sequence. By applying, etc., “dots, lines, aggregates of these, etc.” (hereinafter referred to as “display”) are applied, and on the other hand, only the base sequence substance is bonded, and a signal (for example, light emission, current, electromagnetic wave, The same applies to the sound, etc.), and when the authenticity of the article is determined, the other base sequence substance is applied to the article, and the other base sequence substance The authenticity of the article is determined by detecting the presence or absence of transmission. In addition, in the title of the present specification, this paragraph 0001 and the following description, and the description in the abstract, “on-site” is used to mean “in-situ” and “DNA ink”. Does not transmit alone, and does not mix with or bind to other base sequence substances, but mixes nucleic acid substances based on base nucleic acid sequences that exist alone (hereinafter referred to as “fixed nucleic acids”). Means ink.

  2. Description of the Related Art Conventionally, there is a system (for example, Japanese Patent Application Laid-Open No. 2006-77191) that applies an ink using a light emitting phosphor with a wavelength that is not visible to an article and determines the authenticity of the article using the presence or absence of the light emission. In addition, there is one that performs quantitative analysis of a nucleic acid base sequence based on an orthonormal sequence of the DNA base sequence (for example, JP-A-2006-211982). Furthermore, there is known a technique (for example, Japanese Patent Publication No. 2003-525429) that observes a mark pattern attached to a solid object and detects the presence or absence of a mark by the appearance of the pattern.

JP 2006-77191 A Japanese Patent Laid-Open No. 2006-211982 Special table 2003-525429 gazette

  The invention described in Patent Document 1 can be used for the same authenticity determination even when the composition of the light-emitting phosphor is known, or when another light-emitting phosphor is used. This means that if there is a luminescent phosphor, it is easy to impersonate the authenticity of the article and cannot be used as the authenticity determination of the original authentic article.

  In the invention described in Patent Document 2, a nucleic acid having a new base sequence is formed from an orthonormal sequence and used. The orthonormal sequence is designed, for example, by calculating a continuous match length, a melting temperature prediction, a Hamming distance, and a secondary structure prediction for a base DNA sequence obtained from a random number. An orthonormal sequence is a sequence of nucleic acid molecules having a uniform melting temperature, that is, a sequence designed so that the melting temperature is within a certain range, and binding to its complementary base sequence It is a sequence that does not form a stable secondary structure that inhibits the activity, and has a property that does not form a stable bond other than its complementary base sequence. Can't have the ability.

  The invention described in Patent Document 3 requires a video observation device or the like to detect the appearance of a mark pattern, and the mark cannot be detected on-site within 5 seconds by the detection. In addition, since the mark size needs to be a certain size in order to draw the mark with particles, since the present invention does not use particles, a smaller mark, for example, a size of several micrometers can be drawn. Application to is possible. Furthermore, since the present invention can detect marks at room temperature instead of 37 ° C., it does not require a heating device and can be used for articles that are vulnerable to heat.

  In the present invention, DNA ink is applied to an article requiring authenticity determination. In this state, the article does not transmit, and it is unknown to a third party that the article has been subjected to a measure for authenticity determination. On the other hand, another base sequence substance (hereinafter referred to as “detection nucleic acid”) having the property of binding only to the fixed nucleic acid and transmitting when the binding is performed is prepared. When it is necessary to determine the authenticity of the article, by applying the detection nucleic acid on the fixed nucleic acid, the fixed nucleic acid and the detection nucleic acid are bound to each other, and the detection nucleic acid immediately starts transmission. . An object of the present invention is to provide an on-site article authentication system using DNA ink for judging whether or not an article is authentic within 5 seconds on-site.

(Claim 1) An on-site article authentication system using a DNA ink according to the present invention includes a DNA ink containing an immobilized nucleic acid and a detection nucleic acid that binds only to the immobilized nucleic acid.

  Since the detection nucleic acid binds only to the fixed nucleic acid, if one of the fixed nucleic acid, for example, is applied to an article, and the detection nucleic acid is detected by bringing the detection nucleic acid into contact with the other, for example, the presence or absence of binding. The identity of the article can be determined. Since the fixed nucleic acid and the detection nucleic acid have a property of emitting a signal, for example, light immediately when the binding is performed, when it is necessary to determine the authenticity of the article, the detection nucleic acid is bound to the fixed nucleic acid. Thus, it can be examined whether or not the detection nucleic acid emits a signal on site.

(Claim 2) The binding between the fixed nucleic acid and the detection nucleic acid may be performed immediately before detection.
There are various timings for determining the authenticity of the article. However, if it is performed on the spot where the determination is necessary, an immediate determination is possible, and it is easy to match the authenticator and the detector. The authenticator holding the detection nucleic acid can authenticate on-site at any place at any time.

(Claim 3) The binding between the fixed nucleic acid and the detection nucleic acid may be performed immediately after the fixed nucleic acid is affixed or applied to the article immediately before the detection for article authentication.
In this way, since the detection nucleic acid is always transmitted, when it is necessary to determine the authenticity of the article, the determination operation can be performed only by detecting the presence or absence of transmission.

(Claim 4) The immobilized nucleic acid may be immobilized on a solid phase of the article or an accessory to the article.
In this way, the immobilized nucleic acid is not easily detached from the article or the accessory, and is effective as an authenticity determination means for an article having a long life.

(Claim 5) The fixed nucleic acid may be a normal orthogonal sequence or a sequence that does not interfere with the specific detection of the fixed nucleic acid.
In this way, due to the characteristics of the orthonormal sequence, the detection nucleic acid can be specifically detected by the detection nucleic acid. Further, the detection nucleic acid is prevented from non-specifically binding to the solid phase of the article or the accessory by using a fixed nucleic acid different from the fixed nucleic acid, and the selective binding of the detection nucleic acid to the fixed nucleic acid is prevented. It becomes possible to detect reliably.

(Claim 6) The fixed nucleic acid may include a dummy fixed nucleic acid having the same length as the fixed nucleic acid and having a sequence that does not interfere with detection of the base nucleic acid sequence by the detection nucleic acid.
In this way, in order to make the candy article appear to be a genuine article, the fixed nucleic acid is peeled off and the base sequence of the fixed nucleic acid is analyzed, and the same base sequence substance is forged and applied to the article. By mixing the dummy immobilizing nucleic acid in the DNA ink, it is possible to prevent the distribution of fakes by preventing the identification of the immobilizing nucleic acid that is the object of authenticity determination.

(Claim 7) The detection nucleic acid may have a property of specifically binding only to a specific fixed nucleic acid.
In this way, the detection nucleic acid binds only to the fixed nucleic acid applied on the article that is the object of authenticity determination, and does not bind to other fixed nucleic acids, so the authenticity of the article can be reliably determined.

(Claim 8) The detection nucleic acid may have a property of transmitting only when bound to a specific fixed nucleic acid.
In this way, since the detection nucleic acid is selectively bound to the target fixed nucleic acid and transmitted, the authenticity of the article can be determined by whether or not the detection nucleic acid transmits.

(Claim 9) The detection nucleic acid is a complementary sequence of an orthonormal sequence possessed by the fixed nucleic acid, a sequence having performance equivalent to that of the complementary sequence in the specific detection of the fixed nucleic acid, or a sequence that does not interfere with the specific detection of the fixed nucleic acid. You may have.
This prevents the detection nucleic acid from non-specifically binding to the solid phase of the article or the accessory by a detection nucleic acid different from the detection nucleic acid, and selectively binds the detection nucleic acid to the fixed nucleic acid. In order to ensure that the detection nucleic acid is detected more reliably, and to prevent the detection nucleic acid from being produced for the purpose of making the product appear to be a genuine product, it is possible to effectively distribute counterfeits. Can be prevented.

(Claim 10) In addition to the detection nucleic acid for detecting the base nucleic acid sequence of the fixed nucleic acid, the detection nucleic acid has a base nucleic acid sequence having the same length as that of the detection nucleic acid, and a base based on the detection nucleic acid. A dummy detection nucleic acid having a sequence that does not interfere with detection of the nucleic acid sequence may be included.
In this way, even if a base sequence of the detection nucleic acid is prepared, a large number of nucleobases having the same base sequence as the detection nucleic acid substance are mixed with DNA ink to prepare a dummy detection nucleic acid. Thus, identification of the detection nucleic acid as a detection substance for authenticity determination can be prevented, and the circulation of fakes can be effectively prevented.

(Claim 11) A plurality of types of the fixed nucleic acid are prepared, and one or more types are further selected as the selected fixed nucleic acid, and a detection nucleic acid simultaneously containing a type of detection nucleic acid corresponding to the selected fixed nucleic acid is provided. May be detected.
In this way, since there are a variety of uses for determining the authenticity of an article, a single type of fixed nucleic acid cannot be used, but a plurality of types of selected fixed nucleic acids can be obtained by selectively mixing a plurality of types of base sequences. By adopting the detection nucleic acid corresponding to this, it is possible to meet the demands for a wide variety of applications. For example, when M types of selected fixed nucleic acid are used, an authentication system having 2 ^M −1 types of selected fixed nucleic acid species can be provided.

(Claim 12) A plurality of different types of indications may be given to the article or the accessory by each different DNA ink that selectively contains one or more of the selectively immobilized nucleic acids.
Thereby, the reliability and safety | security of an authentication system can be improved. For example, rather than detecting one type of the display applied using one type of the DNA ink and determining the authenticity of the article, a plurality of different types of the type applied using the plurality of different types of DNA inks are used. If the display is detected and the authenticity of the article is determined, the reliability of authentication can be improved. In addition, if a plurality of different types of display are applied to the article using a plurality of different types of DNA inks in advance, even if the display is counterfeited, the different display is detected. Then, if the authenticity of the article is determined, the article can be continuously authenticated without using another different type of DNA ink to display another different kind of display on the article. Therefore, the security of authentication is improved. This is particularly effective in authentication when it is difficult to repeatedly display the article using the DNA ink.

(Claim 13) The detection nucleic acid bound to the fixed nucleic acid may be rendered undetectable by a control process after detection by the detection nucleic acid.
This is convenient when there is no need for detection because the article has already been sold. In order to make it undetectable, a method of removing the detection nucleic acid with the removed nucleic acid, and in the case where the emission is luminescence, the detection nucleic acid is further coated with a substance that hinders the luminescence ability of the detection nucleic acid to emit the light. There are a method of stopping, a method of eliminating the light emitting ability of the detection nucleic acid by strong light, and the like.
For example, when selling an article as a product, the buyer is witnessed to determine the authenticity of the product, and after confirming that the buyer is authentic, the transmission is stopped and the product is delivered to the buyer. It can be determined that the property has been owned by the buyer, and if the transmission is continued, it can be estimated that the item has been stolen.

(Claim 1) According to the on-site article authentication system using the DNA ink according to the present invention, since the detection nucleic acid binds only to the fixed nucleic acid, for example, the fixed nucleic acid is applied to the article, and the detection nucleic acid is applied to the detection nucleic acid. The identity of the article can be determined on-site by contacting the immobilized nucleic acid and detecting whether or not they are bound.

  According to claim 2, when it is necessary to determine the authenticity of the article, the detection nucleic acid is applied to the article on the spot, and the authenticity of the article can be determined simply, easily and immediately, It is easy to match the authenticator and the detector, and the authenticator holding the detection nucleic acid can authenticate at any time and at any place.

  According to claim 3, since the detection nucleic acid is in a state of always transmitting, when it is necessary to determine the authenticity of the article, the determination work can be performed only by detecting the presence / absence of transmission at any time on the spot. Yes.

  According to the fourth aspect of the present invention, the immobilized nucleic acid does not easily peel off from the article or the accessory, and is effective as an authenticity determination means for an article having a long life.

  According to claim 5, it is possible to prevent nonspecific binding of the detection nucleic acid by a fixed nucleic acid different from the fixed nucleic acid, and to reliably detect the selective binding of the detection nucleic acid to the fixed nucleic acid. It becomes.

  According to claim 6, for the purpose of making the bag article appear to be a genuine article, the fixed nucleic acid is peeled off, the base sequence of the fixed nucleic acid is analyzed, and the same base sequence substance is forged and applied to the article. However, since a large amount of the dummy fixed nucleic acid and the DNA ink are mixed, the identification of the fixed nucleic acid that is the object of authenticity determination can be prevented, and the circulation of fakes can be effectively prevented.

  According to claim 7, since the detection nucleic acid binds only to the fixed nucleic acid coated on the article to be authenticated, and does not bind to other fixed nucleic acids, the authenticity of the article can be reliably determined. Yes.

  According to the eighth aspect, since the detection nucleic acid is selectively combined with the target fixed nucleic acid and transmitted, the authenticity of the article can be determined by whether or not the detection nucleic acid transmits.

  According to claim 9, a nucleobase having a base sequence complementary to the detection nucleic acid substance is prepared, mixed with the detection nucleic acid substance with DNA ink, and the fixed nucleic acid is non-specifically bound by another detection nucleic acid. Can prevent the detection nucleic acid from being selectively bound to the fixed nucleic acid, and can prevent the detection nucleic acid from being identified as a detection substance for authenticity determination. Can be prevented.

  According to claim 10, a dummy detection nucleic acid having a base sequence of the same fluorescent molecule as that of the detection nucleic acid substance and having a sequence that does not prevent detection of the base nucleic acid sequence by the detection nucleic acid is used as a DNA ink. To prevent the identification of the detection nucleic acid as a detection substance for authenticity determination and effectively prevent the distribution of fake.

According to the eleventh aspect, even if the uses for determining authenticity are diverse due to the variety of articles, a plurality of types of fixed nucleic acids can be provided by selectively mixing a plurality of types of fixed nucleic acids, and a wide variety of authentication is determined. Can also be supported. If M types of fixed nucleic acids are used, an authentication system having 2 ^M −1 types of fixed nucleic acid species can be provided, and an authentication system that can be widely applied to authenticity determination of articles can be provided.

  According to the twelfth aspect, since it is possible to determine the authenticity of an article using a plurality of different types of display made using a plurality of different types of the DNA inks, Compared with the case where authenticity determination is performed, a highly reliable authentication system can be provided. In addition, even if the display used for authentication is stolen, it is possible to detect another display different from the display and determine the authenticity of the article. Authentication can be continued without applying to the article. Thus, an effective authentication system can be provided even when it is difficult to add a new display using another type of DNA ink, such as an article after being shipped.

  According to claim 13, since the product purchased as a genuine product by the buyer in the presence of the product can be stopped immediately after the sale by the control process, it becomes evidence that the normal sale has been performed and the transmission is made. If it continues, it is presumed that it has been stolen, and it can be used as an authentication system for theft prevention.

It is a flowchart of the on-site article authentication system using the DNA ink 2 concerning this invention. It is a conceptual diagram of the binding reaction between the fixed nucleic acid 1 of the display 7 and the detection nucleic acid 3 by the DNA ink 2 applied to the article 6 or the accessory 6 '. It is a flowchart which makes the DNA ink 2 select one kind of fixed nucleic acid 1 from the several fixed nucleic acid 1a produced | generated using the method of an orthonormal arrangement | sequence. It is explanatory drawing which mixed the fixed nucleic acid 1 and the some dummy fixed nucleic acid 1b to make the DNA ink 2, and accommodated in the container 2 '. It is explanatory drawing of the state which mixed the detection nucleic acid 3 and the some dummy detection nucleic acid 3a, and accommodated in the container 9. FIG. It is a flowchart which selects the some fixed nucleic acid 1c from the some fixed nucleic acid 1a produced | generated using the method of an orthonormal arrangement | sequence, and accommodates it in the container 2 '. It is a flowchart of the state which performs the control process 11 from the transmission state which the fixed nucleic acid 1 and the detection nucleic acid 3 couple | bonded, and stops transmission. It is a figure which shows the Example of DNA ink 4 second detection.

Embodiments of the present invention will be described below with reference to the drawings.
(Claim 1) In FIG. 1 and FIG. 2, the article authentication system based on the DNA ink according to the present invention comprises DNA containing a fixed nucleic acid 1 obtained by analyzing a base nucleic acid sequence obtained by analyzing a DNA base as a basic seed. It consists of an ink 2 and a detection nucleic acid 3 that binds only to the fixed nucleic acid 1.

The DNA ink 2 obtained by mixing the immobilized nucleic acid 1 generated using the method of the orthonormal arrangement with the ink base material is printed or applied to the article 6 by using the printing machine 4 or the inkjet machine 5 and the display 7 is given. On the other hand, the detection nucleic acid 3 binds only to the fixed nucleic acid 1 and emits light 8 when it is bound, for example, emits light, and is stored in a container 9.
When the authenticity of the article 6 is determined, the detection nucleic acid 3 is applied to the display 7 of the article 6 by the detection nucleic acid applicator 10.

  By this operation, in the article 6 to which the DNA ink 2 is attached, the fixed nucleic acid 1 and the detection nucleic acid 3 are combined to perform transmission 8. When this article authentication system detects this transmission 8, it is proved that the article 6 is genuine. On the other hand, in the article 6 on which the fixed nucleic acid 1 is not coated with the DNA ink 2, even if the detection nucleic acid 3 is coated, no binding occurs, and therefore no transmission 8 is performed. For this reason, by detecting the presence / absence of the transmission 8, it is possible to authenticate on site whether or not the article 6 is a genuine product.

(Claim 2) The fixed nucleic acid 1 and the detection nucleic acid 3 are bound immediately before detection.
In this case, since the fixed nucleic acid 1 and the detection nucleic acid 3 are transmitted 8 in about 4 seconds after they are combined, it can be said that the time zone that exceeds 4 seconds and is closest to the 4 seconds is immediately before. Therefore, when it is necessary to determine the authenticity of the article 6, the detection nucleic acid 3 is applied to the display 7 of the article 6 on the spot, so that the authenticity of the article 6 can be determined simply, easily, and immediately. It is easy to make the person and the detector coincide with each other, and the authenticator holding the detection nucleic acid 3 can authenticate at any time and at any place.

(Claim 3) The immobilization of the fixed nucleic acid 1 and the detection nucleic acid 3 is performed immediately after the immobilization of the fixed nucleic acid 1 to the article 6 before or just before the detection for the article authentication.
In this case, since the detection nucleic acid 3 is always transmitted by the binding with the fixed nucleic acid 1, the authenticator can authenticate at any place as long as it has a device or instrument for detecting the transmission. Can be done.

(Claim 4) The immobilized nucleic acid 1 is immobilized on the solid phase of the article 6 or an accessory 6 'to the article 6.
In this case, the immobilized nucleic acid 1 does not easily peel off from the article 6 or the accessory 6 ′, and is effective as a means for determining the authenticity of the article 6 having a long lifetime. Examples of the attachment 6 ′ include a seal and a cloth piece.

(Claim 5) In FIG. 3, the immobilized nucleic acid 1 is an orthonormal sequence or a sequence that does not interfere with the specific detection of the immobilized nucleic acid. For example, it is generated by an orthonormal sequence designed by calculating a continuous match length, melting temperature prediction, Hamming distance, and secondary structure prediction for a DNA sequence of a base obtained from a random number.
In this case, the detection nucleic acid 3 is prevented from non-specifically binding to the solid phase of the article 6 or the accessory 6 ′ by the fixed nucleic acid 1b different from the fixed nucleic acid 1 by the detection nucleic acid 3, and the detection nucleic acid 3 is fixed. The selective binding to the nucleic acid 1 can be reliably detected.

(Claim 6) In FIG. 4, the fixed nucleic acid 1 includes a dummy fixed nucleic acid 1b having the same length as the fixed nucleic acid 1 and having a sequence that does not interfere with the detection of the base nucleic acid sequence by the detection nucleic acid 3. .
In this case, if there is only one type of fixed nucleic acid 1 applied to the article 6, the fixed nucleic acid is peeled off and analyzed for the purpose of making it appear as if it is a genuine article. It is conceivable to forge the substance and apply it to the article, but it has the same base length as the fixed nucleic acid 1 in the DNA ink 2 containing the fixed nucleic acid 1, but does not bind to the detection nucleic acid 3 and thus transmits. Forgery of the fixed nucleic acid 1 can be prevented by preparing a large number of dummy fixed nucleic acids 1b mixed with nucleic acids that do not exhibit 8.

(Claim 7) The detection nucleic acid 3 has a property of specifically binding only to the specific fixed nucleic acid 1.
In this case, if the fixed nucleic acid 1 changes, the detection nucleic acid 3 does not bind and the transmission 8 is not sent out, so that the authenticity of the article 6 can be easily determined.

(Claim 8) The detection nucleic acid 3 has a property of transmitting only when it is bound to a specific fixed nucleic acid 1.
In this case, since the authenticity of the article 6 is determined based on whether or not the detection nucleic acid 3 is transmitted, the determination is easy.

(Claim 9) The detection nucleic acid 3 is a complementary sequence of an orthonormal sequence possessed by the fixed nucleic acid 1, a sequence having performance equivalent to that of the complementary sequence in the specific detection of the fixed nucleic acid 1, or the specific detection of the fixed nucleic acid 1. It has an array that must not be.
In this case, by using the complementary base sequence, it becomes possible to easily and reliably bind to the fixed nucleic acid sequence, and the authenticity of the article 6 can be reliably determined.

(Claim 10) In FIG. 5, the detection nucleic acid 3 has, in addition to the detection nucleic acid for detecting the base nucleic acid sequence of the fixed nucleic acid 1, a base nucleic acid sequence of the same length and the same fluorescent molecule as its own base nucleic acid sequence, and A dummy detection nucleic acid 3a having a sequence that does not interfere with detection of the base nucleic acid sequence by the detection nucleic acid is included.
In this case, since the dummy detection nucleic acid 3a does not bind to the fixed nucleic acid 1 and thus does not transmit, the detection nucleic acid 3 can be prevented from being counterfeited.

(Claim 11) In FIG. 6, a plurality of types 1a of the fixed nucleic acid 1 are prepared, and one or more types are further selected from these to make a selected fixed nucleic acid 1c. This selective fixed nucleic acid 1c is detected using a detection nucleic acid that simultaneously contains detection nucleic acids of the type corresponding to them.
In this case, since the articles 6 requiring authentication have a wide variety of types and properties, the detection nucleic acid 3 applied for authentication of each type, property, manufacturer or seller also requires a plurality of types. However, by appropriately combining one or more kinds of fixed nucleic acids from a plurality of types 1a to form a selective fixed nucleic acid 1c, and preparing a DNA ink 2 in which the selected fixed nucleic acid 1c is mixed, it is possible to meet the demand. If M types of fixed nucleic acids are prepared, the maximum number of detection types of 2 to the power of M-1 is possible.

(Claim 12) A plurality of different types of indications 7 are provided on the article 6 or the accessory 6 'by each of the different DNA inks 2a that selectively contain one or more of the selectively immobilized nucleic acids 1c.
In this case, as shown in FIG. 8, three types of DNA inks 2 (D1-16, D1) including different types of fixed nucleic acids 1 further selected from a plurality of types of fixed nucleic acids 1 having an orthonormal sequence. -27, D1-44), three types of different displays 7 are given to the substrate which is the article 6. When a detection nucleic acid solution containing a detection nucleic acid 3 that emits light only by binding to the fixed nucleic acid 1 contained in the DNA ink 2 of D1-27 is applied and detected, the “x” applied using the DNA ink 2 of D1-27 is detected. A display 7 in the form of "" is detected. Further, when a detection nucleic acid solution containing a detection nucleic acid 3 that emits light by binding only to the fixed nucleic acid 1 contained in the DNA ink 2 of D1-44 was applied and detected, the DNA ink 2 of D1-44 was applied. A display 7 in the shape of “◯” is detected. If the authenticity of the article 6 is determined using the display 7 having these two different shapes, the reliability of authentication is improved as compared with the case where the determination is performed using one display. Also, for example, when authentication is performed using the “x” -shaped display 7 made using the DNA ink 2 of D1-27, the “x” -shaped display 7 is stolen. Even if it has been counterfeited, if it is changed to perform authentication using the display 7 in the shape of “O” that was previously applied to the article 6 using the DNA ink 2 of D1-44, Since the authenticity of the article 6 can be determined, highly secure authentication is possible.

(Claim 13) In FIG. 7, the detection nucleic acid 3 bound to the fixed nucleic acid 1 is made undetectable by the control process 11 after the detection nucleic acid 3 is detected.
In this case, after the authenticity of the article 6 is determined, the transmission of the detection nucleic acid 3 can be stopped by applying the control process 11 to the detection nucleic acid 3.

＊5’-アミノ修飾ＤＮＡが末端で基板に固定されている。 FIG. 8 shows the detection state of the fixed pattern applied to the article using the DNA ink according to the present invention for each time.
The name, length and arrangement of the DNA ink are as shown in Table 1 below.

* 5'-Amino modified DNA is fixed to the substrate at the end.

* Type of DNA ink on fixed pattern Black circle: D1-44
Light circle: D1-27
Scarlet circle: D1-16
Karamemaru: D1-44 + D1-27
White circle: no ink

* Experiment protocol The DNA ink fixed on the substrate was detected in a room temperature environment using a detection solution (1xSSC, 0.1% SDS) containing 1 μmol of Cy5-terminal modified linear DNA. The linear DNA sequence is a sequence complementary to the DNA ink sequence. The detection procedure is as follows.

“Experiment 1” 20 microliters (μL) of a detection solution is dropped on a region where DNA ink is fixed, and after reacting for 2 seconds, the solution is blotted with a wiping cloth for 2 seconds and immediately detected.

“Experiment 2” μL is dropped onto the area where the DNA ink is fixed, and after reacting for 2 seconds, the solution is sucked off with a wiping cloth for 2 seconds. Thereafter, 20 μL of a cleaning solution (1 × SSC, 0.1% SDS) is dropped, and after waiting for 2 seconds, the cleaning solution is blotted with a wiping cloth for 2 seconds and immediately detected.

“Experiment 3” The process up to the dripping and sucking of the liquid is the same. Thereafter, the cleaning liquid is dropped again, and after waiting for 2 seconds, the cleaning liquid is sucked and detected immediately.

Detection can be performed with the naked eye using a simple fluorescence detection device using an LED light source and a dichroic mirror. The detection image in the figure of the example is obtained by a fluorescence image scanner.
As can be seen from this figure, in the case of Experiment 1, the X mark by D1-27 and the ◯ mark by D1-44 are detected 4 seconds after the start of the experiment. It can be seen that the X mark and the ◯ mark are more clearly detected as 8 seconds in Experiment 2 and 12 seconds in Experiment 3 are passed.

DESCRIPTION OF SYMBOLS 1 Fixed nucleic acid 1a Plural kinds 1b Dummy fixed nucleic acid 1c Selection fixed nucleic acid 2 DNA ink 2 'Container 3 Detection nucleic acid 3a Dummy detection nucleic acid 4 Printing machine 5 Inkjet machine 6 Article 6' Attachment 7 Display 8 Transmission 9 Container 10 Coating machine 11 Control processing

Claims (13)

An on-site article authentication system using a DNA ink comprising a DNA ink (2) containing a fixed nucleic acid (1) and a detection nucleic acid (3) that binds only to the fixed nucleic acid (1). The on-site article authentication system using DNA ink according to claim 1, wherein the binding between the fixed nucleic acid (1) and the detection nucleic acid (3) is performed immediately before detection. The binding between the fixed nucleic acid (1) and the detection nucleic acid (3) is performed immediately after the fixed nucleic acid (1) is applied or applied to the article (6) immediately before the detection for article authentication. An on-site article authentication system using the DNA ink according to 1. The on-site using the DNA ink according to claim 1, wherein the immobilized nucleic acid (1) is immobilized on a solid phase of the article (6) or an attachment (6 ') to the article. Item authentication system. The on-site article authentication system using DNA ink according to claim 1, wherein the immobilized nucleic acid (1) is an orthonormal sequence or a sequence that does not interfere with specific detection of the immobilized nucleic acid. The fixed nucleic acid (1) comprises a dummy fixed nucleic acid (1b) having the same length as the fixed nucleic acid (1) and having a sequence that does not interfere with detection of the base nucleic acid sequence by the detection nucleic acid (3). An on-site article authentication system using the DNA ink according to one of items 1 to 5. The on-site article authentication system using the DNA ink according to claim 1, 2, 3 or 6, wherein the detection nucleic acid (3) has a property of specifically binding only to a specific fixed nucleic acid. The on-site article authentication system using the DNA ink according to claim 1, 2, 3, 6, or 7, wherein the detection nucleic acid (3) has a property of transmitting only when bound to a specific fixed nucleic acid. . The detection nucleic acid (3) is a complementary sequence of an orthonormal sequence possessed by the fixed nucleic acid (1), a sequence having the same performance as a complementary sequence in the specific detection of the fixed nucleic acid (1), or the fixed nucleic acid (1) An on-site article authentication system using the DNA ink according to claim 1, 2, 3, 6, 7, or 8 having a sequence that does not interfere with the specific detection of. The detection nucleic acid (3) has, in addition to the detection nucleic acid for detecting the base nucleic acid sequence of the fixed nucleic acid (1), the base nucleic acid sequence of the same length and the same fluorescent molecule as its own base nucleic acid sequence and their detection. The on-site article using the DNA ink according to claim 1, 2, 3, 6, 7, 8 or 9, comprising a dummy detection nucleic acid (3a) having a sequence that does not prevent detection of a base nucleic acid sequence by a nucleic acid. Authentication system. A plurality of types (1a) of the fixed nucleic acid (1) are prepared, and one or more types are further selected from them to form a selected fixed nucleic acid (1c), and a type corresponding to the selected fixed nucleic acid (1c) is detected. The on-site article authentication system using the DNA ink according to any one of claims 1 to 10, wherein detection is performed using a detection nucleic acid simultaneously containing a nucleic acid. A plurality of different types of indications (7) are displayed on the article (6) or the accessory (6 ′) by each separate DNA ink (2a) that selectively contains one or more of the selectively immobilized nucleic acids (1c). An on-site article authentication system using the DNA ink according to claim 11. The detection nucleic acid (3) bound to the fixed nucleic acid (1) is rendered undetectable by a control process (11) after detection by the detection nucleic acid (3). An on-site article authentication system using the DNA ink according to one of the items.

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