JP2012501187A5 - - Google Patents
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- JP2012501187A5 JP2012501187A5 JP2011525249A JP2011525249A JP2012501187A5 JP 2012501187 A5 JP2012501187 A5 JP 2012501187A5 JP 2011525249 A JP2011525249 A JP 2011525249A JP 2011525249 A JP2011525249 A JP 2011525249A JP 2012501187 A5 JP2012501187 A5 JP 2012501187A5
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- JP
- Japan
- Prior art keywords
- embryos
- mosm
- embryo
- medium
- lipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002257 embryonic structure Anatomy 0.000 claims description 100
- 210000001161 mammalian embryo Anatomy 0.000 claims description 61
- 238000000034 method Methods 0.000 claims description 40
- 150000002632 lipids Chemical class 0.000 claims description 37
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 34
- 238000000338 in vitro Methods 0.000 claims description 21
- 210000002459 blastocyst Anatomy 0.000 claims description 17
- 238000005138 cryopreservation Methods 0.000 claims description 17
- 239000011780 sodium chloride Substances 0.000 claims description 17
- 229930006000 Sucrose Natural products 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 14
- 238000004017 vitrification Methods 0.000 claims description 13
- 210000004340 zona pellucida Anatomy 0.000 claims description 12
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethyl sulfoxide Natural products CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 11
- 238000003776 cleavage reaction Methods 0.000 claims description 9
- 230000007017 scission Effects 0.000 claims description 9
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- 238000011084 recovery Methods 0.000 claims description 6
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- 239000000594 mannitol Substances 0.000 claims description 4
- 235000010355 mannitol Nutrition 0.000 claims description 4
- 235000000346 sugar Nutrition 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
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- 238000004321 preservation Methods 0.000 claims description 3
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- 239000005715 Fructose Substances 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
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- 230000003204 osmotic effect Effects 0.000 claims 2
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
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- -1 0.3 M mannitol Chemical compound 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
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- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
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- GBOGMAARMMDZGR-UHFFFAOYSA-N UNPD149280 Natural products N1C(=O)C23OC(=O)C=CC(O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 GBOGMAARMMDZGR-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
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- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
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- GBOGMAARMMDZGR-JREHFAHYSA-N cytochalasin B Natural products C[C@H]1CCC[C@@H](O)C=CC(=O)O[C@@]23[C@H](C=CC1)[C@H](O)C(=C)[C@@H](C)[C@@H]2[C@H](Cc4ccccc4)NC3=O GBOGMAARMMDZGR-JREHFAHYSA-N 0.000 description 2
- GBOGMAARMMDZGR-TYHYBEHESA-N cytochalasin B Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@@H]3/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(=O)O[C@@]23C(=O)N1)=C)C)C1=CC=CC=C1 GBOGMAARMMDZGR-TYHYBEHESA-N 0.000 description 2
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- 230000002727 hyperosmolar Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- 229940049954 penicillin Drugs 0.000 description 2
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- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
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- 239000004310 lactic acid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
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- 210000000056 organ Anatomy 0.000 description 1
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- 239000000725 suspension Substances 0.000 description 1
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- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
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- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
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Images
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19051508P | 2008-08-29 | 2008-08-29 | |
| US61/190,515 | 2008-08-29 | ||
| PCT/US2009/055424 WO2010025404A1 (en) | 2008-08-29 | 2009-08-28 | High-throughput and non-invasive method to vitrify porcine embryos |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012501187A JP2012501187A (ja) | 2012-01-19 |
| JP2012501187A5 true JP2012501187A5 (enExample) | 2013-09-12 |
Family
ID=41721960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011525249A Pending JP2012501187A (ja) | 2008-08-29 | 2009-08-28 | ブタ胚をガラス化する高スループットおよび非侵襲的方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8852078B2 (enExample) |
| EP (1) | EP2331677A4 (enExample) |
| JP (1) | JP2012501187A (enExample) |
| CN (1) | CN102197130A (enExample) |
| AU (1) | AU2009285530A1 (enExample) |
| BR (1) | BRPI0918262A2 (enExample) |
| CA (1) | CA2741754A1 (enExample) |
| CL (1) | CL2011000438A1 (enExample) |
| WO (1) | WO2010025404A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
| US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
| WO2017099865A1 (en) | 2015-12-07 | 2017-06-15 | Coopersurgical, Inc. | Low temperature specimen carriers and related methods |
| US11856947B2 (en) | 2020-02-17 | 2024-01-02 | Cook Medical Technologies Llc | System for automated permeation of a biological material and method of using same |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995005075A1 (en) * | 1993-08-13 | 1995-02-23 | Bresatec Limited | Cryopreservation of porcine embryos |
| US6485959B1 (en) * | 1998-10-07 | 2002-11-26 | Cedars Sinai Medical Center | Cell preconditioning and cryopresevation medium |
| US20040161735A1 (en) * | 1998-11-24 | 2004-08-19 | The University Of Queensland | Cryopreservation of oocytes and embryos and methods for producing animals involving the same |
| US20020045156A1 (en) * | 2000-05-16 | 2002-04-18 | Mehmet Toner | Microinjection of cryoprotectants for preservation of cells |
| US6503698B1 (en) * | 2000-06-16 | 2003-01-07 | The United States Of America As Represented By The Secretary Of Agriculture | Cryopreservation of swine embryos |
| CN1199688C (zh) * | 2003-06-05 | 2005-05-04 | 于靖波 | 促卵巢功能细胞生长素及其制备方法 |
-
2009
- 2009-08-28 BR BRPI0918262A patent/BRPI0918262A2/pt not_active IP Right Cessation
- 2009-08-28 WO PCT/US2009/055424 patent/WO2010025404A1/en not_active Ceased
- 2009-08-28 US US13/061,222 patent/US8852078B2/en not_active Expired - Fee Related
- 2009-08-28 JP JP2011525249A patent/JP2012501187A/ja active Pending
- 2009-08-28 CN CN2009801428076A patent/CN102197130A/zh active Pending
- 2009-08-28 AU AU2009285530A patent/AU2009285530A1/en not_active Abandoned
- 2009-08-28 CA CA2741754A patent/CA2741754A1/en not_active Abandoned
- 2009-08-28 EP EP09810683.4A patent/EP2331677A4/en not_active Withdrawn
-
2011
- 2011-02-28 CL CL2011000438A patent/CL2011000438A1/es unknown
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