JP2012187014A5 - - Google Patents

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JP2012187014A5
JP2012187014A5 JP2011051179A JP2011051179A JP2012187014A5 JP 2012187014 A5 JP2012187014 A5 JP 2012187014A5 JP 2011051179 A JP2011051179 A JP 2011051179A JP 2011051179 A JP2011051179 A JP 2011051179A JP 2012187014 A5 JP2012187014 A5 JP 2012187014A5
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nucleic acid
seq
amino acid
protein
progeny
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JP2011051179A
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JP2012187014A (en
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Priority to JP2011051179A priority Critical patent/JP2012187014A/en
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Claims (6)

以下の（ａ）〜（ｄ）のいずれかに記載の植物細胞の伸長抑制機能タンパク質をコードする核酸により形質転換された矮小化植物又はその子孫を用いることを特徴とする、内在性又は外来性のタンパク質の大量生産方法；
（ａ）配列番号１に示されるアミノ酸配列をコードする核酸、
（ｂ）配列番号１に示されるアミノ酸配列において１若しくは数個のアミノ酸が欠失、置換若しくは付加されたアミノ酸配列をコードする核酸、
（ｃ）配列番号２に示される塩基配列からなる核酸、
（ｄ）配列番号２に示される塩基配列の相補配列とストリンジェントな条件下でハイブリダイズする塩基配列からなる核酸。 Endogenous or exogenous, characterized by using a dwarfed plant or its progeny transformed with a nucleic acid encoding a plant cell elongation-suppressing function protein according to any one of (a) to (d) below: A method for mass production of proteins of
(A) a nucleic acid encoding the amino acid sequence represented by SEQ ID NO: 1,
(B) a nucleic acid encoding an amino acid sequence in which one or several amino acids have been deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 1.
(C) a nucleic acid comprising the base sequence represented by SEQ ID NO: 2,
(D) A nucleic acid comprising a base sequence that hybridizes with a complementary sequence of the base sequence shown in SEQ ID NO: 2 under stringent conditions. 前記（ａ）〜（ｄ）のいずれかに記載の植物細胞の伸長抑制機能タンパク質をコードする核酸により形質転換された矮小化植物又はその子孫を片親とし、所望の外来タンパク質をコードする遺伝子により形質転換した植物又はその子孫を他の片親として、両者を交配して得られた植物又はその子孫に外来タンパク質を生産させることを特徴とする、請求項１に記載の方法。   A dwarf plant or its progeny transformed with a nucleic acid encoding a plant cell elongation-inhibiting function protein according to any one of (a) to (d) above, and having a single parent as a trait by a gene encoding a desired foreign protein The method according to claim 1, wherein the transformed plant or its progeny is used as another single parent, and the plant or its progeny obtained by crossing the two plants is allowed to produce a foreign protein. 前記伸長抑制機能タンパク質が、そのＣ末側に転写抑制ドメインを結合させたキメラタンパク質であることを特徴とする、請求項１又は２に記載の方法。   The method according to claim 1 or 2, wherein the elongation-suppressing functional protein is a chimeric protein having a transcriptional repression domain bound to the C-terminal side thereof. 前記転写抑制ドメインが、「(K/R)LFGV」、「(L/F)DLN(L/F)(X)P」、「(R/K)LFGV」、「(X)(R/K)LFGV(X) 」からなるモチーフ（但し、Ｘは任意のアミノ酸残基を示す。）及び「DLELRL」の中から選択されるいずれかのアミノ酸配列を含むことを特徴とする、請求項３に記載の方法。   The transcription repression domains are `` (K / R) LFGV '', `` (L / F) DLN (L / F) (X) P '', `` (R / K) LFGV '', `` (X) (R / K ) LFGV (X) "(where X represents any amino acid residue) and any amino acid sequence selected from" DLELRL ". The method described. 前記外来タンパク質が抗体タンパク質である、請求項１〜４のいずれかに記載の方法。   The method according to any one of claims 1 to 4, wherein the foreign protein is an antibody protein. 以下の（ａ）〜（ｄ）のいずれかに記載の植物細胞の伸長抑制機能タンパク質をコードする核酸により形質転換された矮小化植物又はその子孫であって、内在性又は外来性のタンパク質の大量生産方法に用いるための形質転換植物又はその子孫；  A dwarfed plant or its progeny transformed with a nucleic acid encoding a plant cell elongation-inhibiting function protein according to any one of the following (a) to (d), and a large amount of endogenous or exogenous protein Transformed plants or their progeny for use in production methods;
（ａ）配列番号１に示されるアミノ酸配列をコードする核酸、(A) a nucleic acid encoding the amino acid sequence represented by SEQ ID NO: 1,
（ｂ）配列番号１に示されるアミノ酸配列において１若しくは数個のアミノ酸が欠失、置換若しくは付加されたアミノ酸配列をコードする核酸、(B) a nucleic acid encoding an amino acid sequence in which one or several amino acids have been deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 1.
（ｃ）配列番号２に示される塩基配列からなる核酸、(C) a nucleic acid comprising the base sequence represented by SEQ ID NO: 2,
（ｄ）配列番号２に示される塩基配列の相補配列とストリンジェントな条件下でハイブリダイズする塩基配列からなる核酸。(D) A nucleic acid comprising a base sequence that hybridizes with a complementary sequence of the base sequence shown in SEQ ID NO: 2 under stringent conditions.
JP2011051179A 2011-03-09 2011-03-09 Highly effective producing method of protein by transformed plant Withdrawn JP2012187014A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2011051179A JP2012187014A (en) 2011-03-09 2011-03-09 Highly effective producing method of protein by transformed plant

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Application Number Priority Date Filing Date Title
JP2011051179A JP2012187014A (en) 2011-03-09 2011-03-09 Highly effective producing method of protein by transformed plant

Publications (2)

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JP2012187014A JP2012187014A (en) 2012-10-04
JP2012187014A5 true JP2012187014A5 (en) 2013-08-01

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