JP2012088201A - Device and method for measuring active oxygen amount - Google Patents

Abstract

PROBLEM TO BE SOLVED: To measure an amount of active oxygen in a whole living body noninvasively and easily.SOLUTION: A device 900 for measuring an active oxygen amount comprises: hydrogen administration means 100 for administering hydrogen water in order to raise a hydrogen concentration in a living body; emitted hydrogen amount measuring means 200 for measuring a hydrogen amount emitted from the living body; active oxygen amount measuring means 300 for measuring an active oxygen amount in the living body from a difference between a hydrogen amount administered to the living body by the hydrogen administration means 100 and the hydrogen amount emitted from the living body that has been measured by the emitted hydrogen amount measuring means 200.

Description

  The present invention relates to an apparatus and method for measuring the amount of active oxygen in a living body.

  In recent years, methods for reducing active oxygen present in the living body, which is one of the causes of diseases such as cancer and lifestyle-related diseases and aging, have been studied. As one method for reducing active oxygen in a living body, for example, Non-Patent Document 1 and Non-Patent Document 2 show that hydrogen is administered into a living body and hydrogen reacts with active oxygen.

  On the other hand, in order to prevent diseases caused by active oxygen as described above, methods for measuring the amount of active oxygen in a living body are required in various fields such as the medical field.

  Conventionally, as a method for measuring the amount of active oxygen in a living body, an electron spin resonance (ESR) method has been used as the most reliable method. In addition to this method, for example, Patent Document 1 discloses a method for measuring the amount of active oxygen by using a chemical substance that emits light by reacting with active oxygen and measuring the amount of light emitted. In addition, a method for measuring the amount of active oxygen by measuring fluorescence generated by reaction with active oxygen using a fluorescent reagent is presented in, for example, Patent Document 2 and Patent Document 3, and a metal complex, active oxygen, For example, Patent Document 4 and Patent Document 5 disclose a method for measuring the amount of active oxygen by measuring the current generated by the above reaction. In the methods described in Patent Documents 1 to 5, blood is collected from a living body and measurement is performed on the collected blood. In addition, a luminescent reagent is applied to the surface of a living tissue such as skin, A method for locally measuring the amount of active oxygen is proposed in Patent Document 6, for example. Further, for example, Patent Document 7 discloses a method for measuring the amount of active oxygen by inserting a needle-like sensor for measuring active oxygen into a living body and bringing it into contact with blood in the living body.

International Publication WO03 / 038429 JP-A-10-332667 International Publication WO2005 / 103282 International Publication No. WO2004 / 074828 International Publication No. WO03 / 045436 JP 2000-333907 A JP 2002-055078 A

Ohsawa I et al., Nature medicine 13,688-694 (2007) Sato Y, Kajiyama S, etc., Biochem Biophys Res Commun, 375 (3), 346-350 (2008)

  However, the ESR method, which is a conventional method for measuring the amount of active oxygen, can identify active oxygen species and measure the concentration thereof, but is not easy to introduce because the apparatus is expensive and large. In addition, complicated pretreatment with a spin trap agent necessary for fixing active oxygen is required. Since the method of measuring the amount of active oxygen by measuring luminescence or fluorescence requires blood collection, it is an invasive and invasive method similar to the method using a needle sensor. The method of applying a reagent to the skin or the like is non-invasive, but can only measure locally, and cannot measure the amount of active oxygen in the whole body.

  In view of the above problems, an object of the present invention is to non-invasively and easily measure the amount of active oxygen in the entire living body.

  An active oxygen amount measuring apparatus according to the present invention includes a hydrogen administration unit that administers hydrogen water to increase the concentration of hydrogen in a living body, an exhaust hydrogen amount measurement unit that measures the amount of hydrogen exhausted from a living body, and a hydrogen administration And means for measuring the amount of active oxygen in the living body from the difference between the amount of hydrogen administered to the living body by the means and the amount of hydrogen exhausted from the living body measured by the means for measuring the amount of exhaust hydrogen. Yes.

  According to the active oxygen amount measuring apparatus according to the present invention, the in vivo activity is determined from the difference between the amount of hydrogen administered to the living body by the hydrogen administration means and the amount of hydrogen exhausted from the living body measured by the exhaust hydrogen amount measuring means. Since the amount of oxygen is measured, the amount of active oxygen in the whole living body can be measured noninvasively and simply.

  In the active oxygen amount measuring apparatus according to the present invention, the exhaust hydrogen amount measuring means includes an expiratory exhaust amount measuring means for measuring the exhaled breath amount of the living body and an expiratory hydrogen concentration measuring means for measuring the hydrogen concentration in the exhaled breath of the living body. The amount of hydrogen exhausted from the living body includes the amount of exhaled breath of the living body measured by the expiratory exhaust amount measuring means during the unit time and the amount of the exhaled breath of the living body measured by the expiratory hydrogen concentration measuring means during the unit time. It is preferable that the product of the hydrogen concentration is calculated by a value obtained by integrating from the time immediately after the mouthpiece or the mask is worn to the end of the measurement of the hydrogen concentration after drinking the hydrogen water.

  In this case, the expiratory hydrogen concentration measuring means may include a breathing circuit including an inhalation part through which a gas to be taken in by the living body passes and a pipe part through which the exhaled air exhausted from the living body passes. .

  Further, in this case, a container for collecting exhaled air exhausted from the living body may be connected to the end of the tube part.

  Moreover, the apparatus which measures a hydrogen concentration may be provided in the pipe part.

  In the active oxygen content measuring apparatus according to the present invention, the expiratory hydrogen concentration measuring means may include a device that intermittently collects exhaled breath in a living body and measures the hydrogen concentration based on the expiratory gas in the container.

  In the active oxygen content measuring apparatus according to the present invention, the active oxygen preferably includes at least one of hydroxyl radical, superoxide, hydrogen peroxide, singlet oxygen, and nitric oxide.

  The method for measuring the amount of active oxygen according to the present invention comprises a step (a) of administering hydrogen water to a living body, a step (b) of measuring the amount of hydrogen exhausted from the living body, and the amount of hydrogen administered to the living body and the living body. A step (c) of measuring the amount of active oxygen in the living body from the difference from the amount of exhausted hydrogen.

  According to the method for measuring the amount of active oxygen according to the present invention, the amount of active oxygen in the living body is measured from the difference between the amount of hydrogen administered to the living body and the amount of hydrogen exhausted from the living body. It becomes possible to measure the total amount of active oxygen.

  In the method for measuring the amount of active oxygen according to the present invention, the step (b) includes a step (b1) of measuring the exhalation amount of the living body and a step (b2) of measuring the hydrogen concentration in the exhalation of the living body, The amount of hydrogen exhausted from the body is calculated by integrating the product of the exhaled breath volume of the living body per unit time and the hydrogen concentration in the living body breath from the end of administration of the hydrogen water until the end of measurement of the hydrogen concentration. It is preferable.

  In this case, in step (b2), the hydrogen concentration in the exhalation of the living body is measured using a breathing circuit having an inhalation part through which a gas to be taken in by the living body passes and a pipe part through which the exhaled air exhausted from the living body passes. May be.

  Further, in this case, exhaled air exhausted from the living body may be collected by a container connected to the downstream end of the tube part, and the hydrogen concentration may be measured based on the exhaled gas in the container.

  Alternatively, the hydrogen concentration may be measured based on the exhaled breath passing through the tube.

  In the method for measuring the amount of active oxygen according to the present invention, in step (b2), exhaled breath of the living body may be intermittently collected in a container, and the hydrogen concentration may be measured based on the exhaled breath in the container.

  In the method for measuring the amount of active oxygen according to the present invention, the active oxygen preferably contains at least one of hydroxyl radical, superoxide, hydrogen peroxide, singlet oxygen, and nitric oxide.

  According to the method for measuring the amount of active oxygen according to the present invention, the amount of active oxygen in the whole living body can be measured noninvasively and simply.

It is a schematic diagram which shows the structure of the active oxygen amount measuring apparatus which concerns on the 1st Embodiment of this invention. It is a schematic diagram which shows the embodiment with respect to the biological body of the active oxygen content measuring apparatus which concerns on the 1st Embodiment of this invention. It is a block diagram which shows the structure of the active oxygen content measuring means of the active oxygen content measuring apparatus which concerns on the 1st Embodiment of this invention. It is a schematic diagram which shows the embodiment with respect to the biological body of the active oxygen content measuring apparatus which concerns on the 2nd Embodiment of this invention. It is a graph which shows the change of the hydrogen concentration in the expiration | expired_air measured in the 1st Example of this invention.

(First embodiment)
An active oxygen amount measuring apparatus and method according to a first embodiment of the present invention will be described with reference to the drawings.

  As shown in FIG. 1, an active oxygen amount measuring apparatus 900 according to the first embodiment of the present invention includes a hydrogen administration unit 100, an exhaust hydrogen amount measuring unit 200, and an active oxygen amount measuring unit 300. A combination of

  The hydrogen administration means 100 has a sealed container 110 in which hydrogen water 120 is stored, and an opening 130 is provided at the top of the sealed container 110, and the opening 130 seals and opens the opening 130. It is closed by a lid 140 that can be repeated. The sealed container 110 is made of a material that does not easily allow hydrogen to pass through, and is preferably an aluminum pouch or the like.

  The hydrogen water 120 is water in which more hydrogen is dissolved than normal water. The hydrogen concentration of the hydrogen water 120 in the sealed container 110 is preferably about 0.2 mM to 0.79 mM (saturated concentration) in order to sufficiently increase the hydrogen concentration in the living body. Further, the hydrogen concentration of the hydrogen water 120 is more preferably about 0.4 mM which is most easily handled when measuring the hydrogen concentration. By allowing the living body to drink such hydrogen water 120, hydrogen can be administered into the living body. The hydrogen concentration of the hydrogen water 120 may change from the time of preparation of the hydrogen water 120 to the time of administration. For this reason, the hydrogen water 120 was administered to the living body and a part of the hydrogen water 120 was taken and placed in a sealed container such as a glass bottle, and shaken for about 30 minutes, for example, so that the gas phase and liquid phase hydrogen reached equilibrium. In a state, it is preferable to take a small amount of gas in the gas phase with a syringe and measure the hydrogen concentration with, for example, a semiconductor hydrogen sensor.

  The exhaust hydrogen amount measuring means 200 includes, for example, a mouthpiece 10 with a one-way valve that can be inserted into the mouth of a living body and serves as an inhalation part through which a gas to be taken in by the living body, and the mouthpiece 10 with a one-way valve. A breathing hydrogen concentration measuring means 70 including a breathing circuit 50 including a downstream pipe 12 as a pipe portion through which the exhausted exhaled gas is connected and a hydrogen concentration measuring device 16 such as a semiconductor hydrogen sensor or a gas chromatography is provided. Yes. Further, the exhaust hydrogen amount measuring means 200 also includes a flow meter 15 as expiratory exhaust amount measuring means.

  As shown in FIG. 2, the mouthpiece 10 with a one-way valve has a mouthpiece portion which is a portion where a living body is attached to the mouth and an airway portion which becomes a gas flow path. An upstream pipe 11 is connected to one end side of the airway part, and a downstream pipe 12 that is a pipe part through which exhaled air exhausted from the living body passes is connected to the other end side. The living body takes in the gas flowing from the upstream pipe 11 to the mouthpiece 10 with a one-way valve, and then the exhaled air discharged by the living body flows to the downstream pipe 12. In order to do this, the mouthpiece 10 with a one-way valve is provided with a valve for flowing gas from the upstream pipe 11 to the downstream pipe 12 in one direction. That is, by this valve, gas flows only from the upstream pipe 11 to the downstream pipe 12 through the mouthpiece 10 with a one-way valve, and does not flow backward. A gas supply device, a reservoir bag, or the like may be connected to the end of the upstream pipe 11 opposite to the mouthpiece 10 with a one-way valve. Therefore, the indoor air may flow into the upstream pipe 11 without connecting anything. Moreover, you may make it the gas for a living body to take in into the mouthpiece 10 with a one-way valve directly flow without providing the upstream pipe 11. Even in this case, only the exhaled breath of the living body flows to the downstream pipe 12 by the valve. A bag 13, which is a container for collecting exhaled breath, is connected to the end of the downstream pipe 12 opposite to the one-way valved mouthpiece 10. For example, polyethylene, polyvinyl fluoride, aluminum, and the like can be used as the material of the bag 13, and it is particularly preferable to use an aluminum bag that is a material that hardly permeates hydrogen, and a Douglas bag or the like. It doesn't matter. Further, the downstream pipe 12 is provided with the flow meter 15, and the flow meter 15 is preferably, for example, a hot-wire flow meter or the like. From the exhaled breath collected in the bag 13, for example, the hydrogen concentration in the exhaled breath is measured using the hydrogen concentration measuring device 16. In addition, when using the mouthpiece 10 with a one-way valve, it is preferable to attach the nose clip 14 which prevents exhalation from leaking from the living body's nose to the living body's nose.

  The active oxygen amount measuring means 300 is a means for calculating the amount of active oxygen by calculating the difference between the measured amount of hydrogen in the breath of the living body and the amount of hydrogen administered to the living body. Specifically, the active oxygen amount measuring means 300 is, for example, as shown in FIG. 3, an administration calculated from the product of the volume of the hydrogen water 120 administered to the living body and the hydrogen concentration measured before administration as described above. The dose hydrogen input unit 310 for inputting the hydrogen amount, the discharged hydrogen amount input unit 320 for inputting the measured hydrogen amount in the expiration of the living body, the dose hydrogen amount input unit 310, and the discharged hydrogen amount input unit 320 are input. And an in-vivo hydrogen amount calculation unit 330 that calculates the amount of hydrogen consumed in the living body from the numerical value. Furthermore, the active oxygen amount measuring means 300 includes an in vivo active oxygen amount calculating unit 340 that calculates the in vivo active oxygen amount from the numerical value calculated by the in vivo hydrogen amount calculating unit 330, and the in vivo active oxygen amount calculating unit. And a display unit 350 for displaying the result calculated by 340. Since the difference between the amount of hydrogen in the breath of the living body and the amount of hydrogen administered to the living body is the amount of hydrogen that has disappeared by reacting with the active oxygen in the living body, the amount of active oxygen can be calculated using this value. it can. The active oxygen here includes, for example, hydroxyl radical, superoxide, hydrogen peroxide, singlet oxygen, nitric oxide and the like.

  Next, a usage mode in which the amount of active oxygen in a living body is measured using such an active oxygen amount measuring apparatus 900 will be described.

  First, the lid 140 of the sealed container 110 is removed from the opening 130, the opening 130 is opened, and the hydrogen water 120 filled in the sealed container 110 is drunk by the living body, whereby hydrogen is administered into the living body. Thereafter, the nose clip 14 is worn on the nose of the living body, and the living body is breathed with the mouthpiece portion of the mouthpiece 10 with the one-way valve held in the mouth. Thereby, when the living body inhales, the gas flowing from the upstream pipe 11 to the mouthpiece 10 with the one-way valve is taken into the living body. When the living body exhausts the taken-in gas, only the exhaled air discharged from the living body flows through the downstream pipe 12. As a result, only the exhalation of the living body is collected in the bag 13 which is a container for collecting exhalation. The exhaled air discharged from the living body may be collected in the bag 13 intermittently every predetermined unit time. The predetermined unit time is preferably, for example, 30 seconds to 10 minutes, particularly 90 seconds to 3 minutes in consideration of the measurement accuracy of the amount of active oxygen and the burden on the living body. The exhaled air discharged from the living body may be collected in the bag 13 intermittently every unit time, and the capacity of the bag 13 may be a capacity that can collect exhaled air within a predetermined unit time. Can be used. In the present embodiment, the configuration in which exhalation is collected in the bag 13 using the breathing circuit 50 including the mouthpiece 10 with the one-way valve and the downstream pipe 12 has been described. May be collected directly in a container such as the bag 13. Moreover, when using the mouthpiece 10 with a one-way valve, it is preferable to attach the nose clip 14 to the nose of the living body, but the one-way valve that covers the mouth and nose instead of the mouthpiece 10 with the one-way valve. An attached mask may be used. In this case, it is not necessary to attach the nose clip 14 to the nose of the living body.

  While collecting the exhaled air discharged from the living body, the exhaled air exhaust amount is measured by the flow meter 15 provided in the downstream pipe 12. However, since the daily expiratory volume at rest is not substantially changed in the same individual, the expiratory volume at rest may be separately measured without providing the flow meter 15.

  The amount of hydrogen discharged from the living body input by the discharged hydrogen amount input unit 320 of the active oxygen amount measuring means 300 is measured by measuring the hydrogen concentration using the hydrogen concentration measuring device 16 with respect to the collected exhaled air. It can be calculated on the basis of the hydrogen concentration and the exhaled exhaust amount. Prior to the administration of hydrogen water to the living body, the hydrogen concentration in the breath of the living body is measured in advance, and the value of this concentration is subtracted from the value of the hydrogen concentration in the breath measured after the administration of the hydrogen water. It is preferable to calculate the hydrogen concentration in the breath due to the administration of water. Specifically, the amount of hydrogen exhausted from a living body is calculated by integrating the product of the concentration of hydrogen in exhaled breath collected during a predetermined unit time and the amount of exhaled breath during a unit time from the start of measurement to the end of measurement. Is done. In other words, the amount of exhaled hydrogen per unit time is calculated by calculating the amount of exhaled hydrogen per unit time by the product of the concentration of exhaled hydrogen collected in a given unit time and the amount of exhaled breath during the unit time. The amount of hydrogen discharged from the living body is calculated by the sum of the above. Here, the amount of hydrogen includes, but is not limited to, general amounts such as mass, volume, concentration, and number of moles.

  Next, the amount of hydrogen administered to the living body is input to the administration hydrogen amount input unit 310, and the amount of hydrogen in the expired air discharged from the living body is input to the discharged hydrogen amount input unit 320. Then, for example, the amount of hydrogen consumed in the living body is calculated from the in-vivo hydrogen amount calculation unit 330 based on the difference between the amount of hydrogen administered to the living body and the calculated amount of hydrogen discharged from the living body. The in vivo active oxygen amount calculation unit 340 obtains, for example, the product of the amount of hydrogen consumed in the living body and a predetermined coefficient, or substitutes the value of the amount of hydrogen consumed in the living body into a predetermined function. Accordingly, the amount of active oxygen in the living body is calculated from the numerical value calculated by the in-vivo hydrogen amount calculating unit 330, and the calculated amount of active oxygen in the living body is displayed on the display unit 350.

  As described above, the amount of active oxygen in the living body is measured. According to the apparatus and method for measuring the amount of active oxygen according to the first embodiment of the present invention, the amount of active oxygen in the whole living body can be measured noninvasively and simply.

(Second Embodiment)
The active oxygen amount measuring apparatus and method according to the second embodiment of the present invention will be described below with reference to the drawings. In addition, in this embodiment, description is abbreviate | omitted about the member same as 1st Embodiment.

  As shown in FIG. 4, in the active oxygen amount measuring apparatus according to the present embodiment, unlike the first embodiment, a flow meter 15 is disposed in a downstream pipe 12 that is a pipe portion through which exhaled air exhausted from a living body passes. In addition, a hydrogen concentration measuring device 16 is connected to the downstream pipe 12 near the flow meter 15. The bag 13 in the first embodiment is not connected to the end of the downstream pipe 12. Moreover, the mask 20 with a one-way valve which covers a mouth and a nose is used instead of the mouthpiece with a one-way valve. As with the mouthpiece with a one-way valve, the one-way valve-equipped mask 20 is provided with a valve that prevents backflow from downstream to upstream. A mouthpiece with a one-way valve may be used instead of the mask with one-way valve 20. The hydrogen concentration measuring device 16 is, for example, a semiconductor hydrogen sensor or the like connected so that exhaled gas flows from the downstream pipe 12. As a result, exhaled gas discharged from the living body flows into the downstream pipe 12, and the exhaled gas volume and the exhaled gas. The hydrogen concentration of can be continuously monitored. In this way, a bag for collecting exhalation is not necessary, but for example, the downstream pipe is used so that indoor air or the like does not enter the vicinity of the region where the hydrogen concentration measuring device 16 is provided in the downstream pipe 12. The length of 12 may be made sufficiently long, or other means may be used. By setting it as such a structure, the amount of active oxygen in a living body can be measured more simply than 1st Embodiment.

  According to the active oxygen amount measuring apparatus and method according to the second embodiment of the present invention, the amount of active oxygen in the whole living body can be measured non-invasively and simply.

(First embodiment)
A first embodiment using the apparatus and method for measuring the amount of active oxygen according to the present invention will be described.

  In this example, the amount of active oxygen in each living body was measured using seven adults as subjects. Measurements were taken early in the morning with the subject hungry.

  In this embodiment, a breathing circuit is used, and the breathing circuit comprises a high-purity air pipe, a gas flow regulator, a reservoir bag, a connector, a mouthpiece with a one-way valve, a bellows hose, and a Douglas bag. In addition, a hot-wire flow meter is provided on the downstream side (exhalation side) of the mouthpiece with the one-way valve of the breathing circuit so that the exhalation exhaust amount can be automatically measured.

  The subject put on a nose clip in a resting position, put a mouthpiece with a one-way valve in his mouth, and performed mouth breathing. First, before drinking hydrogen water, in order to measure the hydrogen concentration (baseline) in exhaled air at normal times, perform 19 minutes of rest ventilation, collect the exhaled exhaled air, and the method described below The hydrogen concentration in exhaled breath was measured by the method of Subsequently, the mouthpiece with a one-way valve is once removed from the mouth, and after drinking 500 mL of 0.4 mM to 0.7 mM hydrogen water for 1 minute, the minute expiration exhalation amount for 60 minutes and the hydrogen concentration in the expiration Was measured. Here, the hydrogen concentration in the exhalation was measured with a hydrogen sensor using a gas chromatograph semiconductor detection method by collecting exhalations from the Douglas bag at intervals of 2 minutes. Immediately before drinking hydrogen water, 50 μL of the hydrogen water to be actually drunk is collected, put into a 22 mL Pyrex (registered trademark) container, immediately closed, and after 30 minutes or more of shaking, the headspace (inside the container) The hydrogen concentration dissolved in the hydrogen water was measured by measuring the hydrogen concentration in the gas phase part). Thus, the amount of hydrogen water actually drunk was calculated. The hydrogen concentration in the head space was measured by a gas chromatograph semiconductor detection method in the same manner as the measurement of the hydrogen concentration in exhaled breath.

  As a result of the measurement, the breath hydrogen concentration curve shown in FIG. 5 was obtained. The total amount of hydrogen exhausted with the administration of hydrogen water is the area between the breath hydrogen concentration curve and the baseline from 0 minutes (immediately after the end of drinking of hydrogen water) to 60 minutes, and 60 minutes of minute exhalation. Calculated as the product of the quantity. However, if there is little undigested food residue due to early morning hunger, there is often little change in the baseline, but the hydrogen concentration at 60 minutes is below the baseline or higher than the baseline. Sometimes it is. For this reason, a regression line (baseline regression line) is obtained from each measured value of the baseline and the hydrogen concentration value at 60 minutes, and the difference between the expiratory hydrogen concentration curve and the baseline regression line is obtained, and this is exhaled. The amount of exhaled hydrogen derived from hydrogen water was determined by the product with the amount. That is, the product of the integral value from 0 minute to 60 minutes of (expiratory hydrogen concentration curve-baseline regression line) and the minute breath exhalation amount for 60 minutes gives the total amount of exhausted hydrogen accompanying administration of hydrogen water. Can be sought. From the above, the amount of hydrogen taken into the living body and reacted with active oxygen can be estimated from the total amount of hydrogen actually taken into the living body and the amount of exhausted hydrogen. As a result, as shown in [Table 1], it was found that an average of 59 ± 9% of the administered hydrogen was exhausted during expiration.

  Other experiments have shown that the total amount of hydrogen exhausted from the skin is negligible, on the order of 0.1% of the administered hydrogen water. It has also been found that hydrogen leakage in drinking water of hydrogen water is no more than 1%. Therefore, it was estimated that about 40% of the administered hydrogen was consumed by active oxygen in vivo. The hydrogen consumption of 7 adults was 29 ± 12 (SD) nmol / kg (body weight) / min or 1.0 ± 0.3 (SD) μmol / m 2 (body surface area) / min. This amount was estimated to correspond to the amount of active oxygen.

(Second embodiment)
The second embodiment using the active oxygen amount measuring apparatus and method according to the present invention will be described below.

  In this example, the change in the amount of active oxygen accompanying exercise load was measured in a 55-year-old healthy adult male. The subject measured in the early hungry state in the same manner as in the first example. Also in this embodiment, a breathing circuit is used, and the breathing circuit is the same as that of the first embodiment except that a gas flow rate regulator having a large flow rate is used.

  The subject first kept on the bicycle ergometer in a resting position (no load, not pedaled) for 70 minutes (before exercise), and then held the pedal at a load of 20 watts for 70 minutes (baseline). Rowing (including 10 minutes for measurement) and then kept in a resting position on the ergometer with no load for 70 minutes (after exercise). The amount of hydrogen in the breath during these times was measured. Baseline measurements before, during and after exercise are taken in 10 minutes, drinking hydrogen water in the last minute, with 0 minutes immediately after the end of drinking water, and 60 minutes thereafter The total amount of hydrogen in the exhalation was determined. The total amount of hydrogen in the exhalation was calculated by the same method as in the first example.

  As a result, as shown in [Table 2], the total amount of hydrogen taken into the living body by hydrogen water is approximately equal to 267 μmol, 261 μmol and 249 μmol before, during and after exercise, respectively. The total hydrogen displacement was 159 μmol, 110 μmol and 215 μmol, respectively, and the hydrogen consumption was 108 μmol, 152 μmol and 34 μmol, respectively. That is, it was found that the hydrogen consumption increased during exercise compared with before exercise, and further decreased after exercise, compared with before exercise.

  The exercise load in the present embodiment is a load level of aerobic exercise corresponding to rapid walking. It is clear that the amount of active oxygen increases during exercise due to increased metabolism in the living body, but it decreases after exercise compared to before exercise. It was.

  The method for measuring the amount of active oxygen according to the present invention can noninvasively and simply measure the amount of active oxygen in the entire living body, and is particularly useful for an apparatus and method for measuring the amount of active oxygen in the living body. is there.

10 Mouthpiece with one-way valve (suction part)
11 Upstream pipe 12 Downstream pipe (pipe part)
13 Bag (container)
14 Nose clip 15 Flow meter (exhalation exhaust gas measuring means)
16 Hydrogen concentration measuring device 20 Mask with one-way valve 50 Breathing circuit 70 Expiratory hydrogen concentration measuring means 100 Hydrogen dosing means 110 Sealed container 120 Hydrogen water 130 Opening 140 Lid 200 Exhaust hydrogen amount measuring means 300 Active oxygen amount measuring means 310 Dosing hydrogen amount Input unit 320 Exhaust hydrogen amount input unit 330 In vivo consumed hydrogen amount calculation unit 340 In vivo active oxygen amount calculation unit 350 Display unit 900 Active oxygen amount measuring device

Claims (14)

Hydrogen administration means for administering hydrogen water to increase the concentration of hydrogen in the living body;
Exhaust hydrogen amount measuring means for measuring the amount of hydrogen exhausted from the living body;
Active oxygen for measuring the amount of active oxygen in the living body from the difference between the amount of hydrogen administered to the living body by the hydrogen administering means and the amount of hydrogen exhausted from the living body measured by the exhaust hydrogen amount measuring means An active oxygen amount measuring apparatus comprising: a quantity measuring means. The exhaust hydrogen amount measuring means includes expiratory exhaust amount measuring means for measuring the exhaled breath amount of the living body, and expiratory hydrogen concentration measuring means for measuring the hydrogen concentration in the exhaled breath of the living body,
The amount of hydrogen exhausted from the living body is the amount of exhaled breath of the living body measured by the expiratory exhaust amount measuring means during a unit time and the amount of the living body measured by the expiratory hydrogen concentration measuring means during the unit time. 2. The active oxygen amount measuring apparatus according to claim 1, wherein the product of the hydrogen concentration in the exhaled breath is calculated by a value obtained by integrating the product from the end of administration of the hydrogen water to the end of measurement of the hydrogen concentration.   3. The breath hydrogen concentration measuring means includes a breathing circuit including an inhalation part through which a gas to be taken in by the living body passes and a pipe part through which exhaled air exhausted from the living body passes. Active oxygen content measuring device.   The active oxygen amount measuring device according to claim 3, wherein a container for collecting exhaled air exhausted from the living body is connected to an end portion on the downstream side of the tube portion.   The apparatus for measuring the amount of active oxygen according to claim 3, wherein a device for measuring a hydrogen concentration is provided in the pipe portion.   The active oxygen according to claim 2, wherein the expiratory hydrogen concentration measuring means includes a device that intermittently collects the exhaled breath of the living body into a container and measures the hydrogen concentration based on the expiratory gas in the container. Quantity measuring device.   The active oxygen includes at least one of hydroxyl radical, superoxide, hydrogen peroxide, singlet oxygen, and nitric oxide, according to any one of claims 1 to 6. The active oxygen amount measuring apparatus described. A step (a) of administering hydrogen water to a living body;
Measuring the amount of hydrogen exhausted from the living body (b);
And (c) measuring the amount of active oxygen in the living body from the difference between the amount of hydrogen administered to the living body and the amount of hydrogen exhausted from the living body. . The step (b) includes a step (b1) of measuring the exhalation volume of the living body, and a step (b2) of measuring a hydrogen concentration in the exhalation of the living body,
The amount of hydrogen exhausted from the living body is the product of the exhalation amount of the living body during the unit time and the hydrogen concentration in the exhalation of the living body during the unit time of the hydrogen concentration from the end of administration of the hydrogen water. The method for measuring the amount of active oxygen according to claim 8, wherein the active oxygen amount is calculated by a value integrated until the end of the measurement.   In the step (b2), the concentration of hydrogen in the exhaled breath of the living body is measured using a breathing circuit including an inhaling part through which a gas to be taken in by the living body passes and a pipe part through which exhaled air exhausted from the living body passes. The method for measuring the amount of active oxygen according to claim 9.   11. The exhaled air exhausted from the living body is collected by a container connected to the downstream end of the pipe part, and the hydrogen concentration is measured based on the exhaled gas in the container. Method for measuring the amount of active oxygen in   The method for measuring the amount of active oxygen according to claim 10, wherein the hydrogen concentration is measured based on exhaled air passing through the pipe portion.   The method for measuring the amount of active oxygen according to claim 9, wherein in the step (b2), exhaled breath of the living body is intermittently collected in a container, and the hydrogen concentration is measured based on the exhaled breath in the container.   The active oxygen includes at least one of hydroxyl radical, superoxide, hydrogen peroxide, singlet oxygen, and nitric oxide, according to any one of claims 8 to 13. The active oxygen content measuring method as described.

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