JP2012055226A - Cell treatment device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cell treatment device for preventing impurities from being mixed therein and also for collecting end products efficiently.SOLUTION: The cell treatment device 1 includes: a container 10 for containing cell suspension; a motor for rotating the container 10 about a rotational axis L; and a filter 15 arranged in the container 10, and allowing the cell suspension to pass therethrough when the pressure is at a predetermined value or more while prohibiting the cell suspension from passing therethrough when the pressure is at a predetermined value or less. The filter 15 divides the container 10 vertically into a first container 11 formed in the upper side and a second container 12 formed in the lower side. The first container 11 has a first inclined surface 21 formed extending upward from the filter 15 in the direction along the rotational axis L and separating from the rotational axis L. The second container 12 has a second inclined surface 22 formed extending upward from the bottom surface 23 in the direction along the rotational axis L and separating from the rotational axis L.

Description

  The present invention relates to a cell processing apparatus.

  Conventionally, a cell processing apparatus is known in which a digestion chamber for performing a digestion step is connected to a centrifugal chamber for performing a centrifugation step (see, for example, Patent Document 1). In this cell treatment apparatus, cells are aspirated and recovered by jetting a washing solution to a cell pellet concentrated by centrifugation in a centrifuge chamber, and loosening it to the bottom of the digestion chamber. The apparatus can be made compact by integrating the chamber for performing the digestion step and the centrifuge chamber in this way.

International Publication No. 2008/133874

  However, in the cell treatment device disclosed in Patent Document 1 described above, the final product is recovered after returning to the bottom of the digestion chamber. Here, since undigested materials and foreign matters are likely to remain in the digestion chamber, there is a disadvantage that these impurities may be mixed into the final product.

  In addition, the above Patent Document 1 also describes that after digestion in the digestion chamber, the digested material is returned again after cleaning the digestion chamber once through an external filter. Takes time, and it takes time and effort to filter the digest with an external filter.

  This invention is made | formed in view of the said situation, Comprising: It aims at providing the cell processing apparatus which can collect | recover final products efficiently while preventing mixing of an impurity.

In order to solve the above problems, the present invention employs the following means.
The present invention includes a container that contains a cell suspension, a motor that rotates the container around a rotation axis, and a cell suspension that is disposed in the container and passes the cell suspension at a predetermined pressure or higher. And a filter that prohibits passage of the cell suspension below, the filter partitioning the inside of the container in the vertical direction, and a first storage portion formed above and a second formed below The first accommodating portion has a first inclined surface formed so as to be spaced apart from the rotational axis toward the upper direction along the rotational axis from the filter. The cell processing apparatus has a second inclined surface formed so that the two accommodating portions are spaced apart from the rotation axis toward the upper side in the direction along the rotation axis from the bottom surface.

  According to the present invention, the inside of the container is partitioned by the filter, and the first storage portion and the second storage portion are formed in the vertical direction. Among these, the biological tissue and the digestive enzyme solution are accommodated in the second accommodating portion disposed below, the motor is operated, and the containers (the first accommodating portion and the second accommodating portion) are moved at low speed around the rotation axis. By rotating, the living tissue is digested and a cell suspension is generated in the second container. That is, the second storage unit functions as a digestion unit that digests a living tissue to generate a cell suspension.

  Then, the cell suspension is rotated at a high speed around the rotation axis so that the cell suspension is rotated upward in the direction along the rotation axis by the centrifugal force. Along the inclined surface formed so as to be separated from the axis), and reaches the filter. Here, the filter is configured to allow the cell suspension to pass above a predetermined pressure, while prohibiting the passage of the cell suspension below a predetermined pressure. Therefore, when the pressure difference between the first storage portion and the second storage portion becomes a predetermined value or more, the cell suspension passes through the filter and enters the first storage portion.

  The cell suspension that has entered the first housing portion is separated from the rotation axis by a centrifugal force toward the first inclined surface (in the direction along the rotation axis from the bottom surface) formed in the first housing portion. (Inclined surface formed on the outer surface) in the direction perpendicular to the rotation axis. And each component in a cell suspension is centrifuged by the difference in specific gravity. Specifically, a group of cells having a large specific gravity is collected on the side surface (outward in the direction perpendicular to the rotation axis) of the first housing part, and supernatants such as lipids and plasma are collected inside. That is, the first storage unit functions as a concentration unit that concentrates the cell suspension.

  As described above, according to the present invention, a cell suspension is generated by digesting a living tissue using a digestive enzyme with respect to the second storage section that is partitioned by a filter and disposed below. The first storage unit disposed above is allowed to function as a concentrating unit that concentrates the cell suspension. In this way, the digestion process and the concentration process can be performed in a closed system using a single device, thus reducing the risk of foreign matter and the like, and eliminating the work of transferring containers, thereby reducing the labor of the operator. It can be omitted.

In the above invention, the predetermined pressure may be a pressure applied to the filter by a cell suspension when the container is rotated at high speed around the rotation axis by the motor.
With such filter characteristics, when the motor is rotated at a high speed, the cell suspension is passed from the second container to the first container, while from the first container to the second container. The cell suspension can be prohibited from passing through and can be stored in the first accommodating portion.

In the above invention, the filter may be made of a water-repellent material, and the aperture of the filter may be 10 μm or more and 100 μm or less.
By configuring the filter in this way, when the motor is rotated at a high speed, the cell suspension is allowed to pass from the second storage portion to the first storage portion, while from the first storage portion to the second storage portion. The cell suspension can be prohibited from passing through and can be stored in the first accommodating portion. Note that the aperture of the filter is preferably 10 μm or more and 100 μm or less, more preferably about 20 μm.

In the above invention, the filter may be provided with a water-repellent coating, and the aperture of the filter may be 10 μm or more and 100 μm or less.
By configuring the filter in this way, when the motor is rotated at a high speed, the cell suspension is allowed to pass from the second storage portion to the first storage portion, while from the first storage portion to the second storage portion. The cell suspension can be prohibited from passing through and can be stored in the first accommodating portion.

In the above invention, the filter may have a plurality of holes of 10 μm or more and 100 μm or less, and a water repellent treatment may be performed around the holes.
By configuring the filter in this way, when the motor is rotated at a high speed, the cell suspension is allowed to pass from the second storage portion to the first storage portion, while from the first storage portion to the second storage portion. The cell suspension can be prohibited from passing through and can be stored in the first accommodating portion.

In the above invention, an injection nozzle that injects the cleaning liquid toward the outside in a direction perpendicular to the rotation axis in the first housing portion, and an opening in the vicinity of the filter in the first housing portion, and is stored on the filter It is good also as providing with the suction nozzle which attracts | sucks the made liquid.
By doing in this way, a washing | cleaning liquid can be sprayed from a spray nozzle to the cell group centrifuged in the 1st accommodating part, and the sprayed cleaning liquid can be attracted | sucked with the impurity in a cell group from a suction nozzle. Thereby, impurities such as digestive enzymes contained in the cell group can be removed, and the cell group can be washed. In addition, by performing the above operation while the motor is rotating at low speed or while it is stopped, the pellet-like cell group pressed on the side surface (outward in the direction perpendicular to the rotation axis) of the first housing portion is loosened with a washing solution. (Resuspended) and can be recovered from the suction nozzle.

In the above invention, a recess may be provided in a substantially central portion of the filter, and the suction nozzle may be open near the bottom of the recess.
In this way, the supernatant on the filter can be collected in the recess and discharged by the suction nozzle. Thereby, the washing | cleaning efficiency of a cell group can be improved. In addition, after washing the cell group, the cell suspension in which the pellet-like cell group is loosened and resuspended can be collected in the recess and collected by the suction nozzle. Thereby, the collection efficiency of a cell group can be improved.

In the above invention, a second suction nozzle may be provided that is provided in the second housing portion, sucks the inside of the second housing portion, and allows the liquid in the first housing portion to pass through the filter. .
By doing in this way, when the pressure difference between the first storage portion and the second storage portion becomes a predetermined value or more due to the suction force of the second suction nozzle, on the filter (in the first storage portion) The supernatant passes through the filter and enters the second accommodating portion. Then, the supernatant that has entered the second container is discharged from the second container to the outside by the second suction nozzle. Thereby, a supernatant can be discharged | emitted efficiently and the washing | cleaning efficiency of a cell group can be improved.

  According to the present invention, it is possible to prevent the contamination of impurities and to efficiently recover the final product.

It is a schematic block diagram of the cell processing apparatus which concerns on one Embodiment of this invention. It is the elements on larger scale of the filter of FIG. It is a figure which shows the state of the digestion process by the cell processing apparatus of FIG. It is a figure which shows the state of the cell suspension transfer process (before filter passage) by the cell processing apparatus of FIG. It is a figure which shows the state of the cell suspension transfer process (after filter passage) by the cell processing apparatus of FIG. It is a figure which shows the state of the centrifugation process (before separation) by the cell processing apparatus of FIG. It is a figure which shows the state of the centrifugation process (after isolation | separation) by the cell processing apparatus of FIG. It is a figure which shows the state which stopped the motor after centrifugation by the cell processing apparatus of FIG. It is a figure which shows the state of the supernatant discharge process by the cell processing apparatus of FIG. It is a figure which shows the state of the washing | cleaning liquid supply process by the cell processing apparatus of FIG. It is a figure which shows the state by which the cell group centrifuged in the cell processing apparatus of FIG. 1 was resuspended. It is a figure which shows the state of the cell collection process by the cell processing apparatus of FIG. It is the elements on larger scale of the filter which concerns on the modification of FIG. It is a schematic block diagram of the cell processing apparatus which concerns on the 1st modification of FIG. It is a schematic block diagram of the cell processing apparatus which concerns on the 2nd modification of FIG.

Hereinafter, a cell treatment apparatus according to an embodiment of the present invention will be described with reference to the drawings.
As shown in FIG. 1, the cell processing apparatus 1 according to the present embodiment includes a container 10 that contains a cell suspension in which cells in a living tissue are isolated, and a motor that rotates the container 10 around a rotation axis L. 20 and a filter 15 disposed in the container 10.

  The container 10 is a sealed container that contains a cell suspension, has a substantially conical shape, and is arranged with its apex (bottom 23) facing downward. The container 10 is rotated around the rotation axis L by the motor 20. Here, the cell suspension is a suspension in which fat-derived cells or the like (cell group) constituting the adipose tissue are isolated and suspended by adding a digestive enzyme to a living tissue such as adipose tissue and stirring. It is.

  In the container 10, a filter 15 is disposed in a substantially horizontal direction (a direction orthogonal to the rotation axis L). Thus, the inside of the container 10 is partitioned in the vertical direction by the filter 15 and is divided into a first accommodating portion 11 formed above and a second accommodating portion 12 formed below.

  The first accommodating portion 11 formed above the container 10 is formed so as to spread upward in the direction along the rotation axis L from the filter 15. That is, the first accommodating portion 11 has the first inclined surface 21 formed so as to be separated from the rotation axis L toward the upper side in the direction along the rotation axis L from the filter 15. Further, a pocket portion 13 formed in a concave shape is provided on the bottom of the side surface of the first housing portion 11 (outward in the direction perpendicular to the rotation axis L). As will be described later, the pocket portion 13 functions as a cell accommodating portion that accommodates a concentrated cell suspension (cell pellet).

An injection nozzle 16 and a suction nozzle 17 are inserted into the first housing portion 11 in the direction along the rotation axis L from the outside.
The ejection nozzle 16 is a nozzle that ejects the cleaning liquid, and has a shape curved in a direction orthogonal to the rotation axis L in the first storage portion 11. By having such a configuration, the injection nozzle 16 injects the cleaning liquid toward the outside in the direction perpendicular to the rotation axis L (the pocket portion 13) in the first housing portion 11.

  The suction nozzle 17 opens in the vicinity of the filter 15 in the first housing portion 11 and sucks the liquid stored on the filter 15. By having such a configuration, the suction nozzle 17 can suck the cleaning liquid ejected by the ejection nozzle 16 together with impurities in the cell group, as will be described later. As will be described later, the suction nozzle 17 is capable of sucking a cell suspension in which the cell group accommodated in the pocket portion 13 is resuspended.

  The second accommodating portion 12 formed below the container 10 is formed so as to spread upward from the bottom surface 23 in the direction along the rotation axis L. That is, the second accommodating portion 12 has the second inclined surface 22 formed so as to be separated from the rotation axis L toward the upper side in the direction along the rotation axis L from the bottom surface 23.

  The second storage unit 12 is provided with a tissue introduction port 18 into which a living tissue such as a fat tissue is introduced, and an enzyme introduction port 19 into which a digestive enzyme solution for digesting fat cells is introduced. Adipose tissue is introduced from the tissue introduction port 18 into the second accommodation unit 12, a digestive enzyme solution is introduced into the second accommodation unit 12 from the enzyme introduction port 19, and the second accommodation unit 12 ( By rotating the container 10) about the rotation axis L, the internal liquid mixture is stirred, digestion of adipose tissue is promoted, and a cell suspension is generated.

  As shown in FIG. 2, the filter 15 is made of a water-repellent material, and has an opening of 10 μm to 100 μm, more preferably 20 μm. Here, the cells in the cell suspension are 10 μm to 20 μm. Therefore, by having the above-described configuration, the filter 15 allows the cell suspension to pass above a predetermined pressure, while prohibiting the passage of the cell suspension below a predetermined pressure.

Here, the predetermined pressure is applied from the second container 12 to the first container 11 by the cell suspension when the container 10 is rotated at high speed around the rotation axis L by the motor 20. Pressure.
By using such a filter 15, when the motor 20 is rotated at a high speed, the cell suspension is passed from the second container 12 to the first container 11, while the motor 20 is rotated at a low speed or When stopped, the passage of the cell suspension can be prohibited and stored in the first accommodating portion 11.

The operation of the cell processing apparatus 1 having the above configuration will be described below.
First, as shown in FIG. 3, the adipose tissue A is introduced from the tissue introduction port 18 into the second accommodation unit 12, and the digestive enzyme solution B is introduced from the enzyme introduction port 19 into the second accommodation unit 12. The second container 12 (container 10) is rotated around the rotation axis L by the motor 20. Thereby, an internal liquid mixture is stirred, digestion of the adipose tissue A by the digestive enzyme liquid B is accelerated | stimulated, and the cell suspension C is produced | generated. That is, the 2nd accommodating part 12 functions as a digestion part which digests a biological tissue and produces | generates the cell suspension C. FIG.

  Next, the container 10 is rotated around the rotation axis L at a high speed by the motor 20. Thereby, as shown in FIG. 4, the cell suspension C in the second storage portion 12 is moved upward along the second inclined surface 22 formed in the second storage portion 12 by centrifugal force. The filter 15 is pushed up. Here, the filter 15 is configured to allow the cell suspension C to pass at a predetermined pressure or higher, while prohibiting the passage of the cell suspension C below the predetermined pressure. Therefore, when the pressure difference between the first storage unit 11 and the second storage unit 12 becomes equal to or greater than a predetermined value, the cell suspension C passes through the filter 15 and is first filtered as shown in FIG. 1 intrudes into the accommodating portion 11.

  In this state, the container 10 is further rotated around the rotation axis L at a high speed by the motor 20. Thereby, as shown in FIG. 6, the cell suspension C that has entered the first housing portion 11 is along the first inclined surface 21 formed in the first housing portion 11 by centrifugal force. Thus, it is moved outward in the direction orthogonal to the rotation axis L (side surface of the first housing portion 11).

  In this state, the container 10 is further rotated around the rotation axis L at a high speed by the motor 20. Thereby, as shown in FIG. 7, in the cell suspension C, each component in the cell suspension C is centrifuged due to the difference in specific gravity. Specifically, a cell group D having a large specific gravity is collected in the pocket portion 13 formed on the side surface (outward in the direction perpendicular to the rotation axis L) of the first accommodating portion 11, and lipid or Supernatant E such as plasma is collected. That is, the first storage unit 11 functions as a concentration unit that concentrates the cell suspension C.

Next, the motor 20 is stopped and the rotation of the container 10 is stopped. As a result, as shown in FIG. 8, the pellet-shaped cell group D remains in the pocket portion 13 formed on the side surface of the first accommodating portion 11, and the supernatant E is stored on the filter 15. .
In this state, as shown in FIG. 9, the supernatant E stored on the filter 15 is sucked by the suction nozzle 17 opened near the filter 15.

Next, as shown in FIG. 10, the cleaning liquid F is ejected from the ejection nozzle 16 curved in the direction orthogonal to the rotation axis L toward the outside of the direction orthogonal to the rotation axis L (the pocket portion 13). At this time, the container 10 may be rotated around the rotation axis L at a low speed by the motor 20.
Thereby, the pellet-shaped cell group D is loosened by the washing solution F and resuspended in the washing solution F. Thereby, as shown in FIG. 11, the resuspended cell suspension G is stored on the filter 15.
By repeating the operations shown in FIGS. 6 to 11, impurities such as digestive enzymes contained in the cell group D can be removed and the cell group D can be washed.

  Next, as shown in FIG. 12, the washing is performed as described above, and the cell suspension G stored on the filter 15 is sucked by the suction nozzle 17 opened near the filter 15. Thereby, the cell suspension G (cell group D) which is the final product that has been washed and concentrated can be recovered.

  As described above, according to the cell processing apparatus 1 according to the present embodiment, the inside of the container 10 is partitioned by the filter 15 and the biological tissue is digested using the digestive enzyme with respect to the second storage unit 12 disposed below. It can function as a digestion unit that generates a cell suspension C by digestion, and the first storage unit 11 disposed above can function as a concentration unit that concentrates the cell suspension C. As described above, since the digestion process and the concentration process can be performed in a closed system using a single device, the risk of foreign matter mixing is reduced, and the labor of transferring the container 10 is eliminated and the labor of the operator is reduced. Can be omitted.

  In addition, an injection nozzle 16 that injects the cleaning liquid F toward the outside in the direction perpendicular to the rotation axis L in the first housing portion 11 and an opening in the vicinity of the filter 15 in the first housing portion 11. And the suction nozzle 17 that sucks the liquid stored in the first storage section 11, the cleaning liquid F is sprayed from the spray nozzle 16 to the cell group D that has been centrifuged in the first storage portion 11, and the sprayed cleaning liquid F is sucked. It can be sucked together with impurities in the cell group D from the nozzle 17. Thereby, impurities such as digestive enzymes contained in the cell group D can be removed, and the cell group D can be washed. In addition, by performing the above operation while the motor is rotating at low speed or while it is stopped, the cell group D pressed in the pocket portion 13 of the first accommodating portion 11 is loosened (resuspended) with the washing solution F, It can be recovered from the suction nozzle 17.

  Further, when the filter 15 is made of a water-repellent material and has an opening of 10 μm or more and 100 μm or less, when the motor 20 is rotated at a high speed, the second storage unit 12 to the first storage unit 11 are used. While the cell suspension C is allowed to pass through, the cell suspension C can be prohibited from passing from the first storage portion 11 to the second storage portion 12 and stored in the first storage portion 11. The aperture of the filter 15 is preferably 10 μm or more and 100 μm or less, more preferably about 20 μm.

The filter 15 may be provided with a water-repellent coating, and the opening may be 10 μm or more and 100 μm or less.
Further, as shown in FIG. 13, the filter 15 may have a plurality of holes 31 of 10 μm or more and 100 μm or less, and a water repellent pattern (water repellent treatment) 32 may be provided around the holes 31. In this case, the filter 15 is formed with a hydrophilic portion 33 except for the holes 31 and the water repellent pattern 32. FIG. 13 is a top view of the filter 15 as viewed from above in the direction along the rotation axis L. Reference numeral 34 denotes the flow of the final product (cell suspension G) from the pocket portion 13 toward the suction nozzle 17. Show.

[First modification]
Next, a first modification of the present invention will be described below.
As shown in FIG. 14, in addition to the configuration of the cell processing apparatus 1 (see FIG. 1), the cell processing apparatus 2 according to the present modified example has a concave portion at a substantially central portion (near the intersection with the rotation axis L). 38 is provided. Further, the suction nozzle 17 extends to the vicinity of the bottom of the recess 38.

  The recess 38 is formed by opening an opening in the upper surface of the filter 15, that is, in the first accommodating portion 11, and functions as a storage portion that collects and stores the liquid on the filter 15. Then, the liquid stored in the recess 38 can be sucked by the suction nozzle 17.

  According to the cell processing apparatus 2 having the above configuration, the supernatant E on the filter 15 can be collected in the recess 38 and discharged by the suction nozzle 17. Thereby, the washing | cleaning efficiency of the cell group D can be improved. In addition, after washing the cell group D, the cell suspension G in which the pellet-like cell group D is loosened and resuspended can be collected in the recess 38 and collected by the suction nozzle 17. Thereby, the collection | recovery efficiency of the cell group D can be improved.

  In the cell treatment device 2 according to this modification, the filter 15 may be configured to be inclined downward from the peripheral portion toward the central portion (concave portion 38). By doing so, the liquid (supernatant E, cell suspension G) on the filter 15 can be efficiently collected in the recess 38, and the washing efficiency of the cell group D and the recovery efficiency of the cell group D are improved. can do.

[Second modification]
Next, a second modification of the present invention will be described below.
As shown in FIG. 15, the cell treatment device 3 according to the present modification includes a suction nozzle (second second) in the vicinity of the bottom 23 of the second storage unit 12 in addition to the configuration of the cell treatment device 1 (see FIG. 1). A suction nozzle) 39 is provided.

  The suction nozzle 39 operates in a state where the cell suspension C is separated into the cell group D and the supernatant E. That is, as shown in FIG. 8, the pellet-shaped cell group D remains in the pocket portion 13 formed on the side surface of the first accommodating portion 11, and the supernatant E is stored on the filter 15. Then, the suction nozzle 39 is operated.

Then, when the pressure difference between the first housing portion 11 and the second housing portion 12 exceeds a predetermined value due to the suction force of the suction nozzle 39, the top of the filter 15 (inside the first housing portion 11). The clean E passes through the filter 15 and enters the second accommodating portion 12.
The supernatant E that has entered the second storage unit 12 is discharged from the second storage unit 12 to the outside by the suction nozzle 39.

  According to the cell processing apparatus 3 having the above configuration, the supernatant E on the filter 15 can be discharged by the suction nozzle 39 provided in the second storage unit 12 through the filter 15. Thereby, the washing | cleaning efficiency of the cell group D can be improved.

As mentioned above, although embodiment of this invention was explained in full detail with reference to drawings, the specific structure is not restricted to this embodiment, The design change etc. of the range which does not deviate from the summary of this invention are included.
For example, in the present embodiment, the container 10 has been described as having a substantially conical shape, but the first inclined surface 21 is provided in the first accommodating portion 11 and the second Any shape may be used as long as the second inclined surface 22 that satisfies the above-described conditions (inclination angle through which the filter 15 passes when rotated at a high speed) is provided in the housing portion 12, such as a triangular pyramid or a quadrangular pyramid. Polygonal pyramids and spheres may be used.

A Adipose tissue B Digestive enzyme solution C Cell suspension D Cell group E Supernatant F Wash solution G Cell suspension L Rotation axis 1, 2, 3 Cell processing device 10 Container 11 First container 12 Second container 13 Pocket portion 15 Filter 16 Injection nozzle 17 Suction nozzle 18 Tissue introduction port 19 Enzyme introduction port 20 Motor 21 First inclined surface 22 Second inclined surface 23 Bottom surface 39 Suction nozzle (second suction nozzle)

Claims (8)

A container containing a cell suspension;
A motor for rotating the container around an axis of rotation;
A filter disposed in the container and passing a cell suspension above a predetermined pressure, while prohibiting passage of the cell suspension below a predetermined pressure,
The filter divides the inside of the container in the vertical direction, and divides the container into a first housing portion formed above and a second housing portion formed below,
The first accommodating portion has a first inclined surface formed so as to be separated from the rotation axis toward the upper direction along the rotation axis from the filter,
The cell processing apparatus which has a 2nd inclined surface formed so that the said 2nd accommodating part may leave | separate from the said rotating shaft toward the direction upper direction along the said rotating shaft from a bottom face.   The cell processing apparatus according to claim 1, wherein the predetermined pressure is a pressure applied to the filter by a cell suspension when the container is rotated at high speed around the rotation axis by the motor. The filter is made of a water-repellent material,
The cell processing apparatus according to claim 2, wherein the opening of the filter is 10 µm or more and 100 µm or less. The filter is provided with a water-repellent coating,
The cell processing apparatus according to claim 2, wherein the opening of the filter is 10 µm or more and 100 µm or less. The filter has a plurality of holes of 10 μm or more and 100 μm or less,
The cell treatment apparatus according to claim 2, wherein a water repellent treatment is performed around the hole. An injection nozzle for injecting the cleaning liquid toward the outside in the direction perpendicular to the rotation axis in the first accommodating portion;
The cell processing apparatus according to claim 1, further comprising: a suction nozzle that opens in the vicinity of the filter in the first housing portion and sucks the liquid stored on the filter. A concave portion is provided in a substantially central portion of the filter,
The cell processing apparatus according to claim 6, wherein the suction nozzle is opened near the bottom of the recess. The cell treatment according to claim 6, further comprising a second suction nozzle that is provided in the second storage portion, sucks the inside of the second storage portion, and passes the filter through the liquid in the first storage portion. apparatus.

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