JP2012050357A5 - - Google Patents
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- JP2012050357A5 JP2012050357A5 JP2010193828A JP2010193828A JP2012050357A5 JP 2012050357 A5 JP2012050357 A5 JP 2012050357A5 JP 2010193828 A JP2010193828 A JP 2010193828A JP 2010193828 A JP2010193828 A JP 2010193828A JP 2012050357 A5 JP2012050357 A5 JP 2012050357A5
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- cells
- stem cells
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（1）ヒト人工多能性幹細胞を、造血因子を含む培地中で培養する工程、
（2）前記（1）で培養したヒト人工多能性幹細胞からCD34+CD43+細胞を分画する工程、
（3）前記（2）で調製したCD34+CD43+細胞分画に、転写因子Sox17をコードする遺伝子を発現させる工程、および
（4）前項工程（3）で調製した細胞を培養する工程。 In vitro (in vitro) amplification method of hematopoietic stem cells and / or hematopoietic progenitor cells comprising the following steps:
(1) culturing human induced pluripotent stem cells in a medium containing hematopoietic factors,
(2) a step of fractionating CD34 + CD43 + cells from the human induced pluripotent stem cells cultured in (1) above,
(3) a step of expressing a gene encoding the transcription factor Sox17 in the CD34 + CD43 + cell fraction prepared in the above (2), and (4) a step of culturing the cell prepared in the step (3) in the previous section.
（1）ヒト人工多能性幹細胞を、ヒト骨形成タンパク質4（bone morphogenetic protein 4；BMP4）およびヒト線維芽細胞成長因子（fibroblast growth factor）を含む培地中で培養する工程、
（2）前記工程（1）で培養した細胞を、さらにヒトBMP4およびヒト血管内皮細胞増殖因子（vascular endothelial growth factor；VEGF）を含む培地中で培養する工程、
（3）前記工程（2）で培養した細胞を、さらにヒトBMP4、ヒトVEGF、ヒトトロンボポエチン（thrombopoietin）およびヒト幹細胞因子（stem cell factor）を含む培地中で培養する工程、
（4）前記工程（3）で培養した細胞からCD34+CD43+細胞を分画する工程、
（5）前記工程（4）で調製したCD34+CD43+細胞分画に、転写因子Sox17をコードする遺伝子を含むレトロウイルスベクターをトランスフェクションする工程、および
（6）前項工程（5）で調製した細胞を、ストローマ細胞上で培養する工程。 In vitro (in vitro) amplification method of hematopoietic stem cells and / or hematopoietic progenitor cells comprising the following steps:
(1) culturing human induced pluripotent stem cells in a medium containing human bone morphogenetic protein 4 (BMP4) and human fibroblast growth factor;
(2) a step of culturing the cells cultured in the step (1) in a medium further containing human BMP4 and human vascular endothelial growth factor (VEGF);
(3) culturing the cells cultured in the above step (2) in a medium further containing human BMP4, human VEGF, human thrombopoietin and human stem cell factor,
(4) a step of fractionating CD34 + CD43 + cells from the cells cultured in the step (3),
(5) Transfecting the CD34 + CD43 + cell fraction prepared in the above step (4) with a retroviral vector containing a gene encoding the transcription factor Sox17, and (6) the cell prepared in the previous step (5) Is cultured on stromal cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010193828A JP5794510B2 (en) | 2010-08-31 | 2010-08-31 | Efficient induction and amplification method of hematopoietic stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010193828A JP5794510B2 (en) | 2010-08-31 | 2010-08-31 | Efficient induction and amplification method of hematopoietic stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012050357A JP2012050357A (en) | 2012-03-15 |
| JP2012050357A5 true JP2012050357A5 (en) | 2013-10-17 |
| JP5794510B2 JP5794510B2 (en) | 2015-10-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010193828A Active JP5794510B2 (en) | 2010-08-31 | 2010-08-31 | Efficient induction and amplification method of hematopoietic stem cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5794510B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2970881A4 (en) * | 2013-03-14 | 2017-01-25 | Children's Medical Center Corporation | Compositions and methods for reprogramming hematopoietic stem cell lineages |
| JP6995750B2 (en) * | 2015-12-03 | 2022-02-04 | ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッド | Methods for Generating Functional Hematopoietic Stem Cells |
| CN110904041A (en) * | 2019-12-05 | 2020-03-24 | 中山大学 | Medicine for promoting hemopoietic stem cell expansion and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2352816B1 (en) * | 2008-11-07 | 2015-06-24 | Sunnybrook Health Sciences Centre | Human progenitor t-cells |
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2010
- 2010-08-31 JP JP2010193828A patent/JP5794510B2/en active Active
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