JP2012007923A - Automatic analysis device and automatic analysis method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To realize an automatic analysis device that allows the improvement of the stability and accuracy of data and detection with high sensitivity by reducing the density difference of solid distribution without enlargement of the device.SOLUTION: Immediately after reagent dispensation into a reaction container 101 which contains a sample, the reaction container 101 is rotated by a driving gear 102 and a passive gear 103 which are rotation mechanisms, and the sample and the reagent are agitated and mixed. High agitation efficiency can be expected because the agitation by the rotation of the reaction container 101 allows a shear layer to be formed over the entire inner wall surface of the reaction container 101 contacted by the mixed liquid. Even if the reagent splatters against the inner wall surface of the reaction container 101 when discharging the reagent into the reaction container 101, the splattered reagent can be collected because the mixed liquid rises gradually by centrifugal force. Therefore the improvement of the stability of the reagent dispensation can be also expected.

Description

  The present invention relates to an automatic analyzer for analyzing the amount of components contained in a sample such as blood or urine.

  Measures the amount of transmitted or scattered light of single or multiple wavelengths obtained by irradiating light from a light source to a reaction solution that is a mixture of a sample or a sample and a reagent as an analyzer that analyzes the amount of components contained in the sample Thus, an automatic analyzer that calculates a component amount from the relationship between the light amount and the concentration is known.

  In the automatic analyzer described in Patent Document 1, optically transparent reaction vessels are arranged circumferentially on a reaction disk that repeatedly rotates and stops, and a transmitted light measurement unit that is arranged in advance during the reaction disk rotation. Thus, the change over time (reaction process data) of the light amount due to the reaction is measured at a constant time interval for about 10 minutes. After completion of the reaction, the reaction vessel is washed by the washing mechanism and used again for analysis.

  There are two main types of reaction in the reaction solution: biochemical analysis using a color reaction between a substrate and an enzyme, and immunoassay using an agglutination reaction due to the binding of an antigen and an antibody. For analysis, measurement methods such as immunoturbidimetry and latex agglutination are known.

  Test items for immunoassay include CRP (C-reactive protein), IgG (immunoglobulin), RF (rheumatoid factor) and the like, and since the concentration of the measurement substance in blood is low, a highly sensitive detection system is required. In the immunoturbidimetric method, an antibody-containing reagent is used to generate an immune complex with a measurement object (antigen) contained in a sample, and these are optically detected to quantify the amount of components. In the latex agglutination method, a reagent containing latex particles sensitized (bound) with an antibody on the surface is used to agglutinate latex particles by antigen-antibody reaction with the antigen contained in the sample, and these are detected optically. Quantify the amount of ingredients.

  There is also an automatic analyzer for measuring the coagulation ability of blood described in Patent Document 2. Blood flows while maintaining fluidity inside blood vessels, but once it bleeds, coagulation factors present in plasma and platelets are activated in a chain, and fibrinogen in plasma is converted into fibrin and deposited. Lead to hemostasis.

  Such blood coagulation ability includes an endogenous one that stops bleeding in tissue and an extrinsic one that stops bleeding due to trauma or the like. The measurement items related to blood coagulation ability include prothrombin time (PT) in the extrinsic blood coagulation reaction test, activated partial thromboplastin time (APTT) in the intrinsic blood coagulation reaction test, and fibrinogen amount (Fbg).

  Each of these items is based on detecting fibrin precipitated by adding a reagent that initiates coagulation by an optical, physical, or electrical technique. As a method using optical means, the reaction solution is irradiated with light, and the fibrin deposited in the reaction solution is detected by measuring the intensity change over time of scattered light or transmitted light, so that the time when fibrin starts to precipitate can be increased. A method of calculating is known. In the blood coagulation automatic analyzer represented by Patent Document 2, the coagulation time of the blood coagulation reaction (particularly Fbg item) is as short as several seconds, so that photometry is required at intervals as short as 0.1 seconds, and the reaction solution Since the reaction vessel cannot be reused by washing once the solution has solidified, the reaction is performed at an independent photometric port, and the reaction vessel is disposable. The automatic analyzer of the present invention also assumes an independent photometric port. In addition, since the reaction time is short and the time for raising the reagent after the reagent is discharged is insufficient, the reagent dispensing mechanism has a reagent temperature raising function (37 ° C.).

U.S. Pat. No. 4,451,433 JP 2000-32286 A

  By the way, the optical means in the above-mentioned immunoaggregation method, latex aggregation method, and blood coagulation analysis are all changes in the number density and size of solids (immunocomplexes, latex aggregates, fibrin, etc.) present in the liquid. Is detected by an optical method. Such a suspension consisting of solid and liquid is more likely to have a density difference in solid distribution than a liquid-only solution (the reaction solution for biochemical analysis is this), and high-sensitivity detection that detects slight signals The fluctuation becomes a big obstacle when doing it.

  On the other hand, the technique of Patent Document 1 performs photometry while moving during rotation, and scans and integrates a certain range to obtain photometric data. Therefore, in the direction of alleviating density fluctuations in the solid distribution. is there. In this case, a method of expanding the scan area by expanding the cell width is also conceivable, but the cross-sectional shape of the cell is rectangular, and it is the reaction that is perpendicular to the optical axis when crossing the optical axis of the irradiated light. Only in the vicinity of the center of the container, the angular relationship with the optical axis is shifted at both ends of the reaction container.

  On the other hand, if the diameter of the reaction table is increased, the end of the reaction vessel can be approximately perpendicular to the optical axis. However, there is a problem that the apparatus becomes large, and there is a limit to enlargement of the scan area.

  In addition, since the technique of Patent Document 2 performs photometry with the reaction vessel stopped, the only way to enlarge the scan area is to enlarge the photometry area. If the photometry area is equivalent to the apparatus of Patent Document 1, It is difficult to enlarge the scanning area beyond that of the apparatus of Patent Document 1.

  In addition, since the light is radiated while the reaction vessel is stopped, the temperature of the reaction solution in the irradiated area rises locally, and bubbles are generated on the inner wall of the reaction vessel, obstructing photometry. In some cases, a temperature gradient is generated in the liquid and the density difference of the solid distribution increases due to convection, both of which greatly affect the accuracy and reproducibility of the data.

  The object of the present invention is to realize an automatic analysis apparatus and an automatic analysis method capable of suppressing the difference in density of solid distribution, increasing the stability and accuracy of data without increasing the size of the apparatus, and performing highly sensitive detection. It is to be.

  In order to achieve the above object, the present invention is configured as follows.

  In the automatic analyzer and the automatic analysis method of the present invention, while rotating the reaction vessel arranged at the photometric position, the reaction vessel is irradiated with light, and transmitted light emitted from the mixed solution of the sample and the reagent in the reaction vessel or Detects scattered light.

  According to the present invention, in the analysis of a mixed solution of a sample and a reagent that is a solid-liquid suspension, the density difference of the solid distribution is suppressed, and the stability and accuracy of data are improved without increasing the size of the apparatus. Thus, an automatic analyzer and an automatic analysis method capable of highly sensitive detection can be realized.

It is a schematic block diagram of the detection system of the automatic analyzer which is one Example of this invention. It is a schematic block diagram of the automatic analyzer which is one Example of this invention. It is sectional drawing of the photometry port in one Example of this invention. It is explanatory drawing of the photometry method in one Example of this invention. It is a figure which shows the concept of the normal photometry state in one Example of this invention. It is a figure which shows the concept of the photometry state of an example in which the bubble in one Example of this invention exists. It is a figure which shows the concept of the photometry state of the other example in which the bubble in one Example of this invention exists. It is a figure which shows the concept of the photometry state of the other example in which the bubble in one Example of this invention exists. It is a figure which shows the concept of the photometry state of the other example in which the bubble in one Example of this invention exists. It is a figure which shows the concept of the photometry state of the other example in which the bubble in one Example of this invention exists. It is an analysis operation | movement flowchart in one Example of this invention. It is a modification of one Example of this invention, and is a figure which shows the example which made the photometry port multi-port.

Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings.

  FIG. 1 is a schematic configuration diagram of a detection system for an automatic analyzer according to an embodiment of the present invention, and FIG. 2 is a schematic configuration diagram of an automatic analyzer according to an embodiment of the present invention.

  In FIG. 1, a sample is dispensed into a reaction vessel 101 having an optically transparent and circular cross-sectional shape by a sample dispensing mechanism 204 (shown in FIG. 2), and then the reaction vessel 101 is dispensed by a reagent dispensing mechanism 204. The reagent is dispensed into Regarding the cross-sectional shape of the reaction vessel 101, a circular shape is preferable, but a polygonal shape may also be used.

  Immediately after the reagent is dispensed into the reaction vessel 101 containing the sample, the reaction vessel 101 is rotated at, for example, 1000 rpm for 2 seconds by the drive gear 102 and the passive gear 103 which are rotating mechanisms, and the sample and the reagent are stirred and mixed. . The stirring method based on the rotation of the reaction vessel 101 can form a shear layer on the entire inner wall surface of the reaction vessel 101 in contact with the mixed solution, and therefore, a large stirring efficiency can be expected as compared to stirring with a spatula or the like.

  In addition, even when reagent splatters on the inner wall of the reaction vessel 101 when the reagent is discharged into the reaction vessel 101, the splattered reagent can be recovered by the rising of the mixed liquid due to centrifugal force, improving the stability of reagent dispensing. We can expect contribution to.

  Regarding the direction of rotation during stirring, in addition to rotating in one direction, forward / reverse rotation is also conceivable. When forward / reverse rotation is performed, stirring efficiency is improved and bubbles are attached to the inner wall of the reaction vessel 101 during reagent discharge. In addition, the effect of detaching bubbles can be expected.

  Light is irradiated to the mixed solution of the sample and the reagent in the reaction vessel 101 from the light source 104 having a single wavelength (LED, laser, etc.) or multiple wavelengths (halogen lamp, etc.), and transmitted light from the mixed solution in the reaction vessel 101 or Scattered light is received by detectors 105a-105b (photodiode, phototransistor, etc.). In the first embodiment of the present invention, the detectors 105a to 105b are arranged on a horizontal plane including the optical axis of the light source 104, and the direction that matches the optical axis and allows detection of transmitted light is 0 °. That is, α ° with respect to the optical axis means that scattered light in the α ° direction can be detected.

  The light received by the detectors 105a to 105b is converted into a photocurrent, amplified by the amplification unit 106, and input to the A / D conversion unit 107 as a light intensity signal. The light intensity signal input to the A / D conversion unit 107 is converted from an analog signal to a digital signal, and stored in the storage unit 108 as photometric data over time.

  The photometric data stored in the storage unit 108 is determined by the determination unit 109 which photometric data is used for density calculation according to a determination procedure described later, and the selected photometric data is transmitted to the calculation unit 110. . The calculation unit 110 calculates the component concentration from the photometric data transmitted from the determination unit 109 and transmits the result to the control unit 111. Then, the control unit 111 outputs the result to the output unit 112. The input unit 113 is a keyboard or the like for inputting various setting contents such as an operator to the control unit.

  Next, in FIG. 2, the reaction container 101 is transferred from the reaction container supply unit 205 to the sample dispensing port 207 by the transfer mechanism 206. The sample dispensing mechanism 204 dispenses a sample from the sample container 202 mounted on the sample disk 203 to the reaction container 101 located at the dispensing port 207. Then, the reaction container 101 is transferred from the dispensing port 207 to the photometric port 201 by the transfer mechanism 206.

  On the other hand, the reagent in the reagent container 208 is dispensed by the reagent dispensing mechanism 209 into the reaction container 101 containing the sample. After dispensing the reagent into the reaction vessel 101, the reaction vessel 101 is rotated by the drive gear 102, the sample and the reagent are mixed, and the reaction is started. After completion of the reaction between the sample and the reagent, when it is difficult to wash the reaction vessel 101 as in the case of the coagulation analysis item, the reaction vessel 101 is discarded to the disposal port 211 by the transfer mechanism 206.

  When the reaction vessel 101 can be washed and reused as in an immunological analysis item, the reaction vessel 101 is washed by the washing mechanism 210 capable of moving in the XY direction and used again for analysis.

  When washing, the reaction vessel 101 is rotated by the drive gear 102, so that an improvement in washing effect can be expected. The reaction container 101 that has been reused a plurality of times is judged to be discarded from information such as the number of uses or the amount of attenuation of the transmitted light quantity in the empty state from the new state, and is discarded to the disposal port 211 by the transfer mechanism 206. The

  The operation control of each mechanism and the determination of the disposal of the reaction vessel 101 are performed by the control unit 111.

  FIG. 3 is a cross-sectional view of the photometric port 201 in one embodiment of the present invention. In FIG. 3, the reaction vessel 101 is gripped by a gripping part 301 having a passive gear 103, and the gripping part 301 is installed in a constant temperature part 303 for maintaining a constant temperature during the reaction between the sample and the reagent via a bearing 302. Has been.

  A pulse motor 304 for rotating the reaction vessel 101 is provided with a drive gear 102, and a drive force is transmitted from the drive gear 102 to the passive gear 103 to rotate the reaction vessel 101. The drive gear 102 is provided with a rotational positioning sensor 305, and the rotational position of the reaction vessel 101 can be controlled. As a rotational drive system of the reaction vessel 101, a configuration using a pulley and a belt is also conceivable.

  The sensor 305 may be installed on the passive gear 103. Or the structure which attaches a marker to the photometry part of the reaction container 101, and grasps | ascertains a rotation position from photometry data is also considered.

  FIG. 4 is an explanatory diagram of a photometric method in one embodiment of the present invention.

  In FIG. 4, the home position of the rotation of the reaction vessel 101 is known by the sensor 305, and in one embodiment of the present invention, one rotation (360 °) is divided into eight equal portions (one equal 45 °) to obtain eight photometric areas. Set. In an embodiment of the present invention, considering a configuration in which one rotation (50 rpm) is performed in 1.2 seconds, the distance that can be scanned for 0.15 seconds in one area and the radius of the reaction vessel 101 is r is r. (Π · r) / 4.

  In an immunoassay, since a reaction for 10 minutes is usually measured, 500 photometric data are obtained in each photometric area. Generally, in an automatic analyzer, the number of photometric data for 10 minutes is about 30 to 100, and in one embodiment of the present invention, more photometric data can be used for concentration calculation.

  In addition, when it is desired to increase the scan area for integration and to further average the photometric data, the photometric area may be set to be wide. For example, it can be a half-round, one-round, or two-round. That is, the concentration of the target substance in the sample is calculated as a single photometric data by summing the light intensity signals of transmitted light or scattered light for half a revolution, one revolution, or a plurality of rotations of the reaction vessel. However, when the photometry area is set to one or more rounds, the countermeasure for noise removal described later cannot be implemented, but the true data can be increased compared with the noise and the S / N ratio can be improved.

  5 to 10 are diagrams showing the concept of various photometric states in one embodiment of the present invention. In immunoassay, a major factor that hinders the accuracy and reproducibility of data is the influence of bubbles adhering to the inner wall of the reaction vessel.

  FIG. 5 is a conceptual diagram of a normal photometric state, and FIG. 6 is a diagram illustrating an example in which irradiation light is largely blocked by bubbles immediately after entering the reaction vessel 101. In the case shown in FIG. 6, the amount of light is reduced for both transmitted light and scattered light. FIG. 7 shows an example in which incident light is blocked by bubbles immediately before passing through the reaction vessel 101, and only the amount of transmitted light decreases.

  FIGS. 8 and 9 are examples in which only the amount of scattered light increases due to the reflected light from the bubbles. FIG. 10 is an example in which only scattered light is blocked by bubbles and the amount of light decreases. 5 to 10 are conceptual diagrams, and the influence on the light amount varies depending on the size and position of the bubbles.

  FIG. 11 is a diagram showing an analysis operation flowchart in one embodiment of the present invention.

  In FIG. 11A, the 100-round determination unit 109 acquires the data stored in the storage unit 108 as the photometric data of the regions 1 to 8 shown in FIG. 4 (step S1).

  Next, the determination unit 109 detects and excludes areas having abnormal values from the photometric data of the areas 1 to 8, and determines a photometric area from which reaction process data is acquired (steps S2a and S3a). Thereafter, photometric data of the selected region is collected until the end of the reaction (step S4), and the photometric data is averaged and the concentration of the target substance is calculated by the calculation unit 110 (step S5, step S6).

  Then, the result is transmitted to the control unit 111, and the control unit 111 outputs the result to the output unit 112 (step S7).

  In step S2a of FIG. 11A, the photometric data of the eight areas are compared, and the photometric data of the area having the abnormal data is excluded using the photometric data outside the range of a certain threshold, for example, 2SD as the abnormal data. . In this case, the photometric data averaged in step S5 can be considered as a representative value for 1.2 seconds (one round), and it can also be considered that no deviation in photometric timing has occurred.

  FIG. 11B is a modified example of the operation flow shown in FIG. Steps S1 and S4 to S7 in FIG. 11B are the same as the example shown in FIG.

  In the example shown in FIG. 11B, photometric data is obtained by simply detecting a region having a maximum value and a minimum value from 100 photometric data and obtaining reaction process data for a region other than the detected region. An area is determined (step S2b, step S3b).

  By the way, although the reaction container 101 is maintained at a certain constant temperature, for example, 37 ° C. by the constant temperature unit 303, the sample and the reagent are usually dispensed at a temperature of 37 ° C. or less. Particularly, in most cases, the reagent is stored in a cold storage in the apparatus at 10 ° C. or lower until immediately before dispensing. Therefore, even if there are small bubbles immediately after reagent dispensing, the volume of the bubbles expands in the process of raising the temperature to 37 ° C.

  Therefore, it is necessary to grasp in advance the temperature rising time up to 37 ° C. and select the light intensity data after reaching that time. In one embodiment of the present invention, as described above, determination is made from photometric data for 100 laps (120 seconds). As a configuration for determining immediately after dispensing, a reagent dispensing mechanism with a reagent temperature raising function may be used.

  A method is also conceivable in which the photometric data of the eight regions are compared and used for density calculation using the photometric data of the median region. FIG. 11C is a modified example of the operation flow shown in FIG. 11A, and is a method used for density calculation using photometric data of a region that becomes a median value. Steps S1, S4, S6, and S7 in FIG. 11C are the same as the example shown in FIG. 11A, and step S5 in FIG. 11A is omitted.

  In FIG. 11C, an area having a median value is detected, and the area having the detected median value is determined as a photometric area for obtaining reaction process data (steps S2c and S3c).

  When the light intensity data of one area having the median value is used, the maximum photometric timing deviation is 1.05 seconds. As a method of reducing the influence of deviation, it is preferable to reduce the number of parts divided.

  However, in the automatic analyzer, for example, when 34 points are measured in 10 minutes, the light measurement interval is 18 seconds, and the median value can be considered as a representative value of 1.2 seconds, and the influence thereof may be considered to be small. it can.

  In blood coagulation analysis, blood coagulation time is calculated by using the time until the light intensity data reaches a certain amount as the coagulation time, and the differentiation of the light intensity data that changes with time by differentiating the light intensity data. There are known a method of setting the time at which the value is maximized as the blood coagulation time, a method of determining the time until the time corresponding to 1 / N of the time at which the differential value of the light intensity data is maximized as the coagulation time, and the like. . In either method, the coagulation time is calculated by capturing the light intensity change, and photometry is usually required at intervals of 0.1 second.

  Therefore, in order to use the same method as in immunoassay, it is necessary to increase the rotation speed of the reaction container. However, if the rotation speed is increased too much, the mixture solution sticks to the reaction container surface due to centrifugal force. is there. Therefore, in the case of blood coagulation analysis, a configuration is considered in which one rotation (75 rpm) is performed in 0.8 seconds, and photometry is performed in each part of 0.1 seconds. In this case, since the reaction process data cannot be prepared for each part, the light intensity data of the part having the abnormal data is removed on the assumption that all the light intensity data is used.

  In addition, since the blood coagulation reaction may be completed in a few seconds, there is no time to wait for the temperature of the reagent in the reaction container, so a reagent dispensing mechanism with a reagent temperature raising function is essential.

  In order to improve the processing capacity of the automatic analyzer, a configuration in which a plurality of analysis ports are provided is conceivable. When the immunoassay and coagulation analysis are shared, a pulse motor is required for each photometric port in order to switch the rotation speed.

  FIG. 12 is a diagram showing an example in which the number of photometric ports is increased only for immune analysis in one embodiment of the present invention. In FIG. 12, a plurality of constant temperature parts 303 are arranged in series, and the passive gear 103 of the constant temperature part 303 is connected via a transmission gear 701. The passive gear 103 of the constant temperature unit 303 located at the end is meshed with the drive gear 102. The drive gear 102 is driven by a pulse motor 304 as shown in FIG.

  The driving force from the pulse motor 304 can be transmitted to all the reaction vessels 101 by the driving gear 102 and the transmission gear 701. The pulse motor 304 has a stop time (for example, 1 second) for each rotation, and repeats the same operation in a cycle of 2.2 seconds when combined with the rotation time.

  During the stop time, the reaction vessel 101 is attached and detached. In the example shown in FIG. 12, the number of light intensity data points is 272 in 10 minutes. Moreover, in the case of the structure shown in FIG. 12, it is necessary to provide a stirring mechanism separately. As a means for transmitting the driving force, a configuration using a pulley and a belt may be used.

  As described above, according to one embodiment of the present invention, the reaction vessel 101 positioned in the photometry port is irradiated with light while being rotated by the rotation drive mechanism (102, 103, 301, 302, 304), Since it is configured to measure light, an automatic analyzer and an automatic analysis method capable of suppressing the difference in density of the solid distribution, increasing the stability and accuracy of data without increasing the size of the apparatus, and performing highly sensitive detection Can be realized.

  DESCRIPTION OF SYMBOLS 101 ... Reaction container, 102 ... Drive gear, 103 ... Passive gear, 104 ... Light source, 105a, 105b ... Detector, 106 ... Amplification part, 107 ... A / D Conversion unit, 108 ... storage unit, 109 ... determination unit, 110 ... calculation unit, 111 ... control unit, 112 ... output unit, 113 ... input unit, 201 ... photometry Port 202 202 Sample container 203 Sample disk 204 Sample dispensing mechanism 205 Reaction container supply unit 206 Transfer mechanism 207 Sample dispensing port 208 ... Reagent container, 209 ... Reagent dispensing mechanism, 210 ... Cleaning mechanism, 211 ... Disposal port, 301 ... Gripping part, 302 ... Bearing, 303 ... Constant temperature part, 304 ... pulse motor, 305 ... sensor, 7010 ... transmission gear

Claims (19)

Light emitted from a light source for irradiating light to a reaction vessel arranged at the photometric position for mixing and reacting the sample and the reagent, and a mixed solution of the sample and the reagent in the reaction vessel arranged at the photometric position In an automatic analyzer having a detector that detects transmitted light or scattered light, and a calculation unit that calculates a concentration of a target substance in the sample from a light intensity signal obtained from the detector,
An automatic analyzer comprising a rotation mechanism for rotating the reaction container disposed at the photometric position.   2. The automatic analyzer according to claim 1, further comprising a control unit that controls a rotation operation of the rotation mechanism, wherein the control unit changes a rotation speed of the rotation mechanism according to a measurement item of a sample. Analysis equipment.   The automatic analyzer according to claim 1, further comprising a control unit that controls a rotation operation of the rotation mechanism, wherein the control unit operates the rotation mechanism to stir the sample and the reagent in the reaction container. Automatic analyzer characterized by   4. The automatic analyzer according to claim 3, wherein the control unit causes the rotation mechanism to repeat forward rotation and reverse rotation to stir the sample and the reagent in the reaction vessel. .   2. The automatic analyzer according to claim 1, further comprising a control unit that controls a rotation operation of the rotation mechanism, wherein the control unit operates the rotation mechanism when cleaning the reaction vessel. .   The automatic analyzer according to claim 1, further comprising: a control unit that controls a rotation operation of the rotation mechanism; and a storage unit that stores a light intensity signal from the detector, wherein the control unit is controlled by the detector. Assuming that the detected light intensity signal is a natural number n, a photometric area is set for every 360 ° / n rotation of the reaction vessel, and the optical intensity signal for each photometric area is stored in the storage unit. An automatic analyzer characterized by causing the calculation unit to calculate the concentration of a target substance in the sample as a total of light intensity signals for each photometric region as photometric data for one time.   7. The automatic analyzer according to claim 6, wherein the control unit controls the calculation unit to average the photometric data for one rotation of the reaction vessel to calculate the concentration.   7. The automatic analyzer according to claim 6, wherein the control unit controls the calculation unit to remove deviation data from photometric data for one rotation of the reaction vessel, and averages other photometric data to obtain a concentration. An automatic analyzer characterized by being operated.   7. The automatic analyzer according to claim 6, wherein the control unit controls the calculation unit to select a median value of photometric data for one rotation of the reaction vessel to perform concentration calculation. Analysis equipment. Light transmitted to a reaction vessel placed at the photometric position for mixing and reacting the sample and the reagent is reacted, and transmitted light or scattering emitted from the mixed solution of the sample and the reagent in the reaction vessel placed at the photometric position In an automatic analysis method for detecting light and calculating the target substance concentration in the sample from the detected light intensity signal,
Irradiating the reaction container with light while rotating the reaction container arranged at the photometric position, and detecting transmitted light or scattered light emitted from a mixed solution of a sample and a reagent in the reaction container. Automatic analysis method to do.   The automatic analysis method according to claim 10, wherein the rotation speed of the reaction vessel is changed according to the measurement item of the sample.   The automatic analysis method according to claim 10, wherein the reaction container is rotated to stir the sample and the reagent in the reaction container.   13. The automatic analysis method according to claim 12, wherein the sample and the reagent in the reaction vessel are agitated by repeatedly rotating the reaction vessel forward and backward.   The automatic analysis method according to claim 10, wherein the reaction vessel is rotated even when the reaction vessel is washed.   11. The automatic analysis method according to claim 10, wherein if the detected light intensity signal is a natural number, a photometric area is set for every 360 ° / n rotation of the reaction vessel, and the optical intensity for each photometric area. An automatic analysis method characterized in that a signal is stored, and the concentration of a target substance in the sample is calculated using the total of the stored light intensity signals for each photometric area as a single photometric data.   16. The automatic analysis method according to claim 15, wherein the concentration calculation is performed by averaging photometric data for one rotation of the reaction vessel.   16. The automatic analysis method according to claim 15, wherein deviation data is removed from photometric data for one rotation of the reaction vessel, and other photometric data are averaged to calculate a concentration.   16. The automatic analysis method according to claim 15, wherein the concentration is calculated by selecting a median value of photometric data for one rotation of the reaction vessel.   The automatic analysis method according to claim 10, wherein the light intensity signals of the transmitted light or scattered light for a plurality of rotations of the reaction vessel are summed, and the target substance concentration in the sample is calculated as a single photometric data. Automatic analysis method characterized by

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