JP2011246358A - Aerosol device for mildew-proofing - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an aerosol device for mildew-proofing capable of storing mildew-proofing microorganisms in an effective state for a long period of time, easily depositing the mildew-proofing microorganisms on the whole of an object for a mildew-proofing treatment uniformly as much as possible to easily obtain a high mildew-proofing effect, and securing safeness of a human body in a mildew-proofing treatment.SOLUTION: The mildew-proofing aerosol device 1 contains: mildew-proofing microorganisms that form spores under prescribed conditions and proliferously grow on a surface of an object for a mildew-proofing treatment to suppress proliferous growth of other fungi; a liquid in which the mildew-proofing microorganisms are mixed; an aerosol container 2 housing the mildew-proofing microorganisms and the liquid; and an inert compressed gas with low chemical reactivity, which fills the aerosol container 2.

Description

  The present invention relates to an anti-mold aerosol device used, for example, when a mold-proof treatment is applied to a bathroom or the like.

  2. Description of the Related Art Conventionally, in bathrooms or the like, it is known that an antifungal agent that prevents the generation of mold is sprayed in a trigger type sprayer (see, for example, Patent Document 1). The antifungal agent of Patent Document 1 is obtained by mixing a water repellent film forming component with a mold repellent component, and the water repellent film is formed after spraying, so that the fungicidal effect lasts for a long period of time. It has come to be.

JP 2006-188468 A

  By the way, for example, in the bathroom, mold may occur not only on the wall surface but also on the ceiling. Therefore, when using the trigger type sprayer as in Patent Document 1 in the bathroom, the user has to operate the trigger many times to spread the antifungal agent from the wall surface to the ceiling, Work is complicated. In particular, since the ceiling is at a high place, it is difficult to disperse the fungicide, or it may be difficult to obtain the fungicide effect desired by the user due to uneven dispersion.

  On the other hand, various microorganisms exist in nature, and among them, the following properties are known particularly for bacteria belonging to the genus Bacillus. That is, when a Bacillus bacterium adheres to an object, it begins to grow on the surface of the object. In the process of Bacillus genus growth, the source of nutrients for other bacteria is not taken in, and the Bacillus bacterium that has grown is present so as to cover the surface of the bacterium, and other bacteria are present on the surface. Suppresses adhesion to objects. By these things, the proliferation of another microbe will be suppressed.

  Therefore, it is conceivable to focus on the above-mentioned properties of the bacteria belonging to the genus Bacillus, and to suppress the generation of mold by growing it on the surface of the mold prevention object. However, since Bacillus bacteria are organisms and cannot be effective when they are killed, how to keep them in an effective state until use becomes a problem. In particular, when it is distributed in the general market, it is preferable that the usable period is longer, and there is a strong demand for a longer period for keeping the bacteria of the genus Bacillus in an effective state.

  In addition, mold prevention treatment is often performed in a closed place, for example, and it is necessary to ensure safety to the human body at the time of treatment.

  The present invention has been made in view of such a point, and the object of the present invention is to prevent mold microorganisms that exhibit fungicidal action, such as Bacillus bacteria, having a durability called spores under certain conditions. Focusing on the point that forms a certain cell structure, it is possible to preserve mold-proof microorganisms capable of obtaining an anti-fungal effect in an effective state over a long period of time by utilizing the property of forming the spore. It is possible to easily obtain a high antifungal effect by attaching microorganisms to the entire antifungal object as easily and as easily as possible, and also to ensure safety to the human body during the antifungal treatment. is there.

  In order to achieve the above object, in the present invention, mold-proof microorganisms are stored in an aerosol container together with a predetermined compressed gas, and the mold-proof microorganisms are jetted from the aerosol container using the compressed gas.

The first invention is
An antifungal microorganism that forms a spore under predetermined conditions and grows on the surface of the antifungal object to suppress the growth of other fungi;
A liquid in which the above fungicidal microorganisms are mixed;
An aerosol container containing the above fungicidal microorganisms and liquid;
An inert compressed gas having a low chemical reactivity and filled in the aerosol container at atmospheric pressure or higher,
The fungus-proof microorganisms are jetted by the compressed gas.

  According to this configuration, the mold-proof microorganism is housed in the aerosol container, so that the mold-proof microorganism is placed in an environment different from the normal growth environment, and the mold-proof microorganism forms a spore. At this time, the aerosol container is filled with an inert gas having low chemical reactivity as a compressed gas for injecting the mold-proof microorganisms. The inert gas does not adversely affect the mold-proof microorganism, and the mold-proof microorganism is hardly killed. Therefore, it is possible to keep the mold-proof microorganisms in an effective state for a long period until use.

  In use, mold-proof microorganisms are jetted from the aerosol container by the compressed gas. Therefore, compared with the case where a trigger type sprayer as in the conventional example is used, it is possible to easily attach the mold-proof microorganisms to the entire surface of the mold-proof object with less unevenness. Since the anti-fungal microorganism attached to the anti-mold object is placed in a normal growth environment, it germinates and proliferates. Thereby, it is possible to suppress the occurrence of mold on the mold prevention object.

  Further, since the gas for injecting the mold-proof microorganism is an inert gas having low chemical reactivity, it has low flammability, no possibility of explosion, and no adverse effect on the human body.

  In addition to the fungi, the anti-fungal microorganisms can grow on the surface of the anti-mold object, and for example, it is possible to suppress the growth of bacteria that cause slimming.

According to a second invention, in the first invention,
The compressed gas is characterized in that the container is filled so that the internal pressure of the aerosol container is 0.7 Mpa or more.

  According to this configuration, the internal pressure of the aerosol container is set to 0.7 Mpa or more, so that the mold-proof microorganisms can be ejected vigorously compared to the case where the pressure is less than that, and the mold-proof object such as the ceiling is less uneven Can be attached.

According to a third invention, in the first or second invention,
The liquid is characterized in that it is a dispersion for dispersing mold-proof microorganisms in an aerosol container.

  According to this configuration, it is possible to disperse the mold-proof microorganisms in the aerosol container without shaking the aerosol container before use. Therefore, it is possible to reliably inject the mold-proof microorganisms from the start to the end of the injection as intended.

According to a fourth invention, in the third invention,
The dispersion contains water and a swellable clay mineral that maintains a dispersed state in water, and both are mixed.

  According to this configuration, the swellable clay mineral swells with water and maintains a state of being dispersed in water. At this time, since it has thixotropic properties, the viscosity increases. And, since the antifungal microorganisms exist between or on the particles constituting the clay mineral, sedimentation of the antifungal microorganisms is suppressed by the clay mineral and can be dispersed inside the aerosol container. become.

  On the other hand, when the swellable clay mineral has thixotropy, when a shear stress is generated in the dispersion after the start of jetting, the viscosity is lowered and the fluidity is improved. Thus, the swellable clay mineral does not hinder the spraying of the mold-proof microorganism.

  In addition, the mold-proof microorganisms are jetted from the aerosol container together with the clay mineral. Therefore, the mold-proof microorganisms adhere to the mold-proof object together with the clay mineral. When mold-proof microorganisms adhere together with clay minerals, for example, when water is splashed on the mold-proof object, the mold-proof microorganisms are prevented from flowing along with the water, and the mold-proof microorganisms. The survival of microorganisms is improved.

According to a fifth invention, in any one of the first to fourth inventions,
The aerosol container is characterized by being a full-volume injection type configured to inject substantially all of the fungicidal microorganisms in a single operation.

  According to this configuration, at the time of the mold prevention treatment, the user can spray almost all of the mold prevention microorganisms from the aerosol container with only one operation, and thus the treatment can be performed more easily. .

In the sixth invention,
The dispersion contains at least one of smectite and mica clay mineral as the swellable clay mineral.

  According to this configuration, among the swellable clay minerals, smectite and mica clay mineral are particularly highly swellable and strong in thixotropy, so that the mold-proof microorganisms are maintained in a dispersed state in the aerosol container. Is possible over a long period of time, and when sprayed, the clay mineral exhibits sufficient fluidity and does not hinder the spraying of mold-proof microorganisms.

In a seventh invention, in any one of the first to sixth inventions,
The aerosol container contains lower alcohol,
The amount of the lower alcohol is set so as to occupy 20% or less of the total volume of contents other than the compressed gas contained in the aerosol container.

  According to this structure, since the germs in the aerosol container are sterilized by the action of the lower alcohol, no off-flavor is generated during use. By setting the amount of the lower alcohol as described above, the antifungal microorganism is hardly adversely affected, and the antifungal microorganism can be kept in an effective state.

  According to the first invention, since the mold-proof microorganisms are contained in the aerosol container and filled with the inert compressed gas having low chemical reactivity, the mold-proof microorganisms are effective for a long period of time by forming spores. It can be stored in a state, and the anti-fungal microorganisms can be easily attached to the whole anti-mold object with little unevenness and easily attached, and a high anti-mold effect can be easily obtained. Safety to the human body can be secured.

  According to the second aspect of the invention, since the internal pressure of the aerosol container is set to 0.7 Mpa or more, the antifungal effect can be further enhanced by adhering the antifungal microorganisms to the antifungal object with little unevenness.

  According to the third invention, the mold-proof microorganisms can be dispersed in the aerosol container, so that the mold-proof microorganisms can be reliably sprayed as intended, and the mold-proof microorganisms are unevenly applied to the mold-proof object. It can be made to adhere in a state with little.

  According to the fourth aspect of the invention, since the dispersion liquid contains water and the swellable clay mineral that maintains the state dispersed in water, sedimentation of mold-proof microorganisms can be suppressed over a long period of time. In addition, since the antifungal microorganisms adhere to the antifungal object together with the clay mineral, the persistence of the antifungal microorganisms can be increased, and the antifungal effect can be continuously obtained over a long period of time. it can.

  According to the fifth aspect of the invention, since the aerosol container is a full-injection type, the mold prevention treatment can be performed more easily.

  According to the sixth invention, among the swellable clay minerals, a clay mineral having a particularly high swellability and strong thixotropy is used. Can be an obstacle during injection, and the mold prevention treatment can be more reliably performed.

  According to the seventh invention, the lower alcohol is accommodated in the aerosol container, and the amount of the lower alcohol is set so as to occupy a volume of 20% or less. Can be sterilized with lower alcohol. Thereby, generation | occurrence | production of a strange odor at the time of use can be suppressed, and it can be set as the anti-mold aerosol apparatus with a favorable usability | use_condition.

It is a perspective view of an aerosol device for mold prevention concerning an embodiment of the present invention. It is a graph which shows the change of the number of bacteria in an aerosol container by the case where nitrogen is filled into an aerosol container, and the case where dimethyl ether is filled. It is a graph which shows the relationship between the internal pressure of an aerosol container, and the number of adhesion bacteria. It is a figure which shows the outline of a test chamber. It is a graph which shows the change of the number of bacteria in an aerosol container at the time of changing the quantity of ethanol.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. It should be noted that the following description of the preferred embodiment is merely illustrative in nature, and is not intended to limit the present invention, its application, or its use.

  FIG. 1 is a perspective view of an anti-mold aerosol device 1 according to an embodiment of the present invention. The anti-fungal aerosol apparatus 1 includes an anti-fungal microorganism, a dispersion liquid (liquid) for mixing the anti-fungal microorganism and dispersing the anti-fungal microorganism, and an aerosol container in which the anti-fungal microorganism and the dispersion liquid are accommodated. 2 and a compressed gas filled in the aerosol container 2.

  The mold prevention aerosol device 1 can be used, for example, in a bathroom, a washroom, etc., and the mold prevention microorganisms are sprayed from the aerosol container 2 by the pressure of the compressed gas so as to adhere to the mold prevention object. It is configured. Examples of the anti-mold object include a wall, a ceiling, and a floor in the case of a bathroom.

  First, the structure of the aerosol container 2 will be described. The aerosol container 2 includes, for example, a pressure-resistant can 3 having water resistance whose inner surface is coated with a resin or the like, and a cap 4 attached to the pressure-resistant can 3 so as to cover a discharge pipe (not shown) of the pressure can 3. And. The discharge pipe is provided at the upper end of the pressure can 3, and therefore the cap 4 is located at the top of the pressure can 3. The internal capacity of the pressure can 3 is, for example, about 300 ml to 400 ml.

  The cap 4 includes a button 5 for operating the discharge pipe and a nozzle 6 communicating with the discharge pipe. The button 5 is configured to operate the discharge pipe in the opening direction when pressed by the user. The cap 4 is provided with a button holding mechanism that can hold the pressed button 5 as it is. That is, the aerosol container 2 is configured such that when the user presses the button 5 once, substantially all of the contents in the aerosol container 2 continue to be injected until the compressed gas runs out even if the user releases the hand. This is a so-called full injection type.

  As the button holding mechanism, a conventionally known one can be used, and for example, a mechanism disclosed in JP-A-2005-298051 can be used.

  Antifungal microorganisms are bacteria of the genus Bacillus. Specifically, Bacillus subtilis. Bacillus subtilis bacteria, including natto bacteria, are harmless to the human body. Bacillus subtilis has a property of forming spores under an environment (predetermined conditions) different from the normal growth environment. In addition, Bacillus subtilis grows when it adheres to the surface of an antifungal object, and has the property of suppressing the adhesion of other fungi and taking in and eliminating the nutrients of other fungi during the growth process. ing. In addition to Bacillus subtilis, bacteria that form spores under predetermined conditions can be used as antifungal microorganisms. For example, Bacillus simplex can be used.

  The dispersion contains water, a swellable clay mineral that maintains a dispersed state in water, and ethanol as a lower alcohol, which are mixed. The water is, for example, ion exchange water.

  When water and a swellable clay mineral that maintains a dispersed state in water are mixed, the swellable clay mineral swells and spreads throughout the water. And since the swellable clay mineral has thixotropic properties, the viscosity of the dispersion increases. When Bacillus subtilis is stirred in water together with the swellable clay mineral, it exists between the particles of the swellable clay mineral or exists on the particles. Thereby, sedimentation of Bacillus subtilis bacteria is suppressed, and Bacillus subtilis bacteria can be dispersed inside the aerosol container 2. Therefore, the swellable clay mineral that maintains a dispersed state in water is suitable as a dispersant for dispersing Bacillus subtilis.

  Hereinafter, the reason why the swellable clay mineral that maintains the dispersed state in water is suitable as a dispersant for dispersing Bacillus subtilis will be described based on experimental results. The experimental results are shown in Table 1.

  In this experiment, the presence or absence of sedimentation of Bacillus subtilis and the presence or absence of fluidity of the dispersant solution (dispersion) were confirmed using various dispersants.

  The experimental method will be described. First, a solution in which each dispersant is dissolved in water is prepared. The amount of the dispersant is an amount suitable for each dispersant. In each solution, 0.4% by weight of a powder preparation of Bacillus subtilis was added and sufficiently stirred. And after accommodating in a predetermined container, it left still at room temperature for about 1 week, and checked the presence or absence of sedimentation of Bacillus subtilis by visual observation, and confirmed fluidity | liquidity.

  As a result, precipitation of Bacillus subtilis was observed because the polymer dispersant was polyvinyl pyrrolidone (trade name: Pitzkor), an anionic surfactant (trade name: Charol), and a nonionic surfactant (trade name). : Neugen). About these three, it experimented by setting the quantity of a dispersing agent to 1.0 weight%, 3.0 weight%, and 5.0 weight% about each, but all are precipitation of Bacillus subtilis bacteria. It was seen. That is, these three dispersants are not preferable as a dispersant for Bacillus subtilis even if the amount is adjusted.

  When Bacillus subtilis settles, it becomes agglomerated and hardened, and there is a problem that it cannot be ejected from the aerosol container 2.

  When acrylic acid (trade name: Carbopol) was used as the polymer dispersant in Table 1, no precipitation was observed. However, the fluidity is poor and it is not preferable for use in the mold prevention aerosol apparatus 1. That is, in the mold prevention aerosol apparatus 1, the dispersant is also ejected from the aerosol container 2, but in the case of carbopol, the fluidity is so bad that it cannot be ejected from the aerosol container 2. Experiments were carried out with the amount of carbopol being 0.1% by weight, 0.5% by weight, and 1.0% by weight.

  Of the swellable clay minerals in Table 1, kaolin (trade name: kaolin) has hydrophilicity but has a property of separating in water. Therefore, precipitation of Bacillus subtilis was observed. Experiments were carried out with the amount of kaolin being 1.0% by weight, 5.0% by weight, and 10.0% by weight. In all cases, precipitation of Bacillus subtilis was observed.

  Among the swellable clay minerals, bentonite (trade name: Kunipia, Bengel), saponite (trade name: smecton), hectorite (product), which are hydrophilic and maintain a dispersed state in water When using name: Laponite B), synthetic smectite (Lucentite), and mica clay mineral (trade name: Somasif), sedimentation of Bacillus subtilis was not observed. In addition, these swellable clay minerals have strong swellability and thixotropic properties. By having thixotropic properties, when shearing stress is generated in the swellable clay mineral after the start of injection, the viscosity decreases, the fluidity becomes better than carbopol, and injection from the aerosol container 2 is easy. Bentonite, saponite and hectorite are a kind of smectite.

  From the above experimental results, it was found that smectite or mica clay mineral is suitable as a dispersant for Bacillus subtilis. Either smectite or mica clay mineral may be used as a dispersant, and a mixture of smectite and mica clay mineral may be used as a dispersant. Also, any two or more of bentonite, saponite and hectorite may be mixed and used as a dispersant.

  Moreover, as shown in the following Table 2, when a swellable clay mineral that maintains a dispersed state in water is used as a dispersant, Bacillus subtilis bacteria that adhere to the mold-proof object after injection are likely to remain.

  This table shows the results of experiments on how the persistence of Bacillus subtilis changes depending on the presence and concentration of the swellable clay mineral.

  The experimental method is as follows. First, three types of test solutions 1 to 3 shown in the following table are prepared.

  The test liquid 1 is a liquid obtained by mixing ion-exchanged water, ethanol, and a powder preparation of Bacillus subtilis. The amount of ion exchange water is 36 ml. Ethanol is 99% synthetic ethanol and the amount is 4 ml. The powder preparation of Bacillus subtilis is 0.16 g.

  Test solution 2 is a solution obtained by adding laponite B (trade name) as a swellable clay mineral to test solution 1. The amounts of ion-exchanged water, ethanol, and Bacillus subtilis powder preparation are the same as in Test Solution 1. The amount of laponite B is 0.2 g, corresponding to 0.5% by weight.

  Test liquid 3 is a liquid in which the amount of laponite B in test liquid 2 is increased. The amount of laponite B is 0.8 g, corresponding to 2.0% by weight.

  250 μl of each of the above test solutions 1 to 3 was applied to the entire surface of a 25 mm square tile and an ABS plate having the same dimensions as a test piece. The number of Bacillus subtilis bacteria in each test piece is measured to obtain the number of applied bacteria. It is assumed that tiles and ABS plates are anti-mold objects.

  The specimen is left overnight after preparation. Then, each test piece is soaked in 100 ml of physiological saline for 30 minutes and then shaken, and then the number of Bacillus subtilis bacteria present in the physiological saline is measured to obtain the number of bacteria dropped from the test piece. .

  The residual ratio in Table 2 is a percentage calculated based on the following formula.

(Number of bacteria applied-number of bacteria dropped) / number of bacteria applied As shown in Table 2, when the swellable clay mineral is not included, Bacillus subtilis is more likely to fall from the test piece than the case where it is included. Is low (poor persistence). Further, when the concentration of the swellable clay mineral is high, the residual rate is increased. This is presumably because the swelling clay mineral acts as a kind of adhesive to prevent Bacillus subtilis from falling off the test piece. In particular, in the case of an ABS plate, the effect of the swellable clay mineral appears remarkably.

  Next, the compressed gas will be described. Nitrogen is used as the compressed gas. The reason will be described based on the experimental results shown in FIG. FIG. 2 shows a case where a liquid corresponding to the test solution 2 is contained in an aerosol container 2 and further filled with nitrogen (plotted with a triangular mark in FIG. 2) and filled with dimethyl ether (marked with the same square). It is a graph which shows the result of having left for 3 months and measuring the number of bacteria every month. The internal pressure of the aerosol container 2 filled with nitrogen and the internal pressure of the aerosol container 2 filled with dimethyl ether are set to 0.4 Mpa. Moreover, the atmospheric temperature of each aerosol container 2 is set to 40 ° C.

  When filled with nitrogen, the change in the number of Bacillus subtilis is small. On the other hand, when dimethyl ether was filled, the number of Bacillus subtilis bacteria was approximately halved after 1 month, and was approximately 0 after 2 months.

  Therefore, it can be seen that the aerosol container 2 containing Bacillus subtilis is preferably filled with nitrogen. Since nitrogen is an inert gas with low chemical reactivity, it does not adversely affect Bacillus subtilis and hardly kills Bacillus subtilis. Therefore, it is possible to keep the Bacillus subtilis bacteria in an effective state for a long period until use.

  The gas filled in the aerosol container 2 may be an inert gas having low chemical reactivity other than nitrogen, and may be, for example, carbon dioxide, nitrous oxide, argon, helium, or the like. Moreover, you may fill the aerosol container 2 with what mixed arbitrary 2 or more types among these gas in arbitrary ratios. An inert gas having a low chemical reactivity is a chemically stable gas that does not easily cause a chemical reaction with other substances. In this specification, as described above, a wide range including elements other than rare gas group elements. Use in concept.

  The internal pressure of the aerosol container 2 is preferably 0.7 Mpa or more. This is because, when it is less than 0.7 Mpa, the amount of Bacillus subtilis adhering to the anti-mold object is greatly reduced as compared with 0.7 Mpa or more.

  This will be described with reference to FIGS. The test solution 2 was placed in an aerosol container 2 and filled with nitrogen. By changing the internal pressure of the aerosol container 2 by changing the filling amount of nitrogen, prepare four mold prevention aerosol devices 1 whose internal pressure is 0.6Mpa, 0.7Mpa, 0.8Mpa, 0.9Mpa, Were injected in a test chamber as shown in FIG. The size of the test chamber is a cubic shape with a side of 186 cm. The inner surface of the test chamber becomes a mold prevention object. The mildew-proof aerosol apparatus 1 was placed in the vicinity of the substantially central portion of the floor surface of the test chamber, and the entire contents were injected. A plurality of agar media are stuck downward on the ceiling of the test room.

As shown in FIG. 3, when the internal pressure is 0.6 Mpa, the number of bacteria in the agar medium is only about 100 cfu / 64 cm ² , whereas the number of bacteria in the case of 0.7 Mpa is that in the case of 0.6 Mpa. It became about 420 cfu / 64 cm ² more than 3 times. As described above, by setting the internal pressure to 0.7 Mpa or more, it is possible to significantly increase the amount of Bacillus subtilis attached to the antifungal object as compared with the case of less than 0.7 Mpa. In addition, since it will become difficult to ensure the pressure resistance of the aerosol container 2 if the internal pressure of the aerosol container 2 is too high, 1.0 MPa or less is preferable.

  The amount of ethanol in the dispersion occupies 20% or less of the total volume of the contents (water, swellable clay mineral, Bacillus subtilis) other than the compressed gas contained in the aerosol container 2. Is set to This is because when it exceeds 20%, Bacillus subtilis decreases with the passage of time. This will be described with reference to FIG.

  FIG. 5 shows that the ethanol amount is 10% (10% ethanol) and 20% of the total volume of the contents contained in the aerosol container 2 (20%). 20% ethanol) and 40% ethanol (40% ethanol) were prepared and left for 3 months, and the number of bacteria was measured every month.

  The aerosol container 2 is filled with nitrogen, and the internal pressure is set to 0.7 MPa. Moreover, the atmospheric temperature of each aerosol container 2 is set to 40 ° C. In 40% ethanol, the number of bacteria gradually decreased, whereas in 10% ethanol and 20% ethanol, the change in the number of bacteria is small.

  Moreover, when ethanol is not added at all, a strange odor may be felt at the time of jetting due to the presence of various germs in the aerosol container 2. In order to sterilize various bacteria, it is preferable to add ethanol, and the minimum amount thereof is preferably an amount occupying 5% or more of the total volume of the contents contained in the aerosol container 2.

  The above-mentioned dispersion and Bacillus subtilis are accommodated in an aerosol container 2 and filled with nitrogen to obtain an anti-mold aerosol device 1. The Bacillus subtilis bacterium housed in the aerosol container 2 is placed in an environment different from the normal growth environment for the Bacillus subtilis bacterium. Thus, Bacillus subtilis forms a spore. At this time, the aerosol container 2 is filled with nitrogen as a compressed gas for injecting Bacillus subtilis. Therefore, the compressed gas does not adversely affect Bacillus subtilis, and Bacillus subtilis is hardly killed. Therefore, as shown in FIG. 2, it is possible to keep the Bacillus subtilis bacteria in an effective state for a long period until use.

  Moreover, the decrease of Bacillus subtilis is also avoided by setting the amount of ethanol to 20% or less as described above. Therefore, the mold-proof aerosol apparatus 1 is suitable for distribution in the general market.

  Next, the usage point of the anti-mold aerosol apparatus 1 comprised as mentioned above is demonstrated. For example, when used in a bathroom, the mold prevention aerosol device 1 is placed on the floor. Then, the button 5 of the cap 4 is pressed. Then, the contents of the aerosol container 2 are ejected from the nozzle 6. Since the pressed button 5 is held as it is, almost all of the contents of the aerosol container 2 are ejected even if left unattended, and the mold prevention treatment can be easily performed.

  The Bacillus subtilis bacteria are dispersed in the aerosol container 2 without being settled by the action of the swellable clay mineral in the dispersion liquid in the aerosol container 2. For this reason, it is possible to disperse Bacillus subtilis in the aerosol container 2 without shaking the aerosol container 2 before use. Therefore, it is possible to reliably inject the Bacillus subtilis bacteria as intended from the start to the end of the injection of Bacillus subtilis.

  Moreover, since the internal pressure is 0.7 Mpa or more, Bacillus subtilis adheres to the ceiling of the bathroom. Further, since nitrogen is used as a compressed gas for injecting Bacillus subtilis, there is no flammability, there is no possibility of explosion, and there is no adverse effect on the user, which is safe.

  When the Bacillus subtilis is sprayed, both swellable clay minerals are sprayed from the aerosol container 2. Therefore, Bacillus subtilis bacteria adhere together with the swellable clay mineral on the wall surface and ceiling of the bathroom, which is the object of mold prevention.

  Bacillus subtilis adheres to the object of mold prevention together with the swellable clay mineral, for example, when water is splashed on the object of mold prevention, Bacillus subtilis will be washed away with the water. Is suppressed by the swellable clay mineral. Thereby, the persistence of Bacillus subtilis is improved.

  Since the Bacillus subtilis bacteria attached to the antifungal object are placed in a normal growth environment, they germinate and start to multiply. In the process of Bacillus subtilis growth, the source of nutrients for other fungi is removed, and the grown Bacillus subtilis is present so as to cover the surface of the fungicidal object. Is prevented from adhering to the mold-proof object. By these things, generation | occurrence | production of mold | fungi is suppressed.

  As described above, according to the mold prevention aerosol device 1 according to this embodiment, since the aerosol container 2 contains Bacillus subtilis and filled with nitrogen, the spore is formed in the Bacillus subtilis. It can be stored in an effective state over a period of time, and Bacillus subtilis can be easily attached to the entire antifungal object with little unevenness and easily with a high antifungal effect. In addition, safety to the human body during mold prevention treatment can be ensured.

  In addition, since the internal pressure of the aerosol container 2 is 0.7 Mpa or more and the aerosol container 2 is a full-injection type, the mold prevention treatment can be performed more easily, and the Bacillus subtilis bacteria are unevenly formed on the mold prevention object. The anti-fungal effect can be further enhanced by adhering in a state where there is a small amount of.

  Moreover, since Bacillus subtilis bacteria can be dispersed in the aerosol container 2, the Bacillus subtilis bacteria can be sprayed as intended, and can be attached to the mold-proof object with little unevenness. .

  Moreover, since the dispersion contains water and a swellable clay mineral, sedimentation of Bacillus subtilis can be suppressed over a long period of time. In addition, since Bacillus subtilis adheres to the antifungal target together with the clay mineral, it is possible to increase the persistence of the antifungal microorganism and to obtain the antifungal effect continuously over a long period of time. Can do.

  Also, among the swellable clay minerals, by using a clay mineral that is particularly highly swellable and has strong thixotropic properties, while maintaining the state in which Bacillus subtilis is dispersed, the dispersion liquid is An obstacle can be avoided, and the mold prevention treatment can be more reliably performed.

  Further, since ethanol is accommodated in the aerosol container 2 and the amount of this ethanol is set to occupy a volume of 20% or less, other bacteria can be sterilized without adversely affecting Bacillus subtilis. Thereby, generation | occurrence | production of a strange odor at the time of use can be suppressed, and it can be set as the mildew-proof aerosol apparatus 1 with a favorable usability | use_condition.

  In the above embodiment, ethanol is accommodated in the aerosol container 2, but the present invention is not limited to this, and any lower alcohol may be used.

  Moreover, as what is accommodated in the aerosol container 2, things other than the above-mentioned thing can also be accommodated.

  Examples of the present invention will be described below.

  The inner volume of the aerosol container 2 was 370 ml.

  The dispersion liquid accommodated in the aerosol container 2 contains water and Laponite B as a swellable clay mineral. The water was ion-exchanged water and the amount was 36 ml. Laponite B was mixed in an amount of 0.2 g. The amount of laponite B is 0.5% by weight. Moreover, ethanol was mixed with the dispersion liquid, the ethanol was 99% synthetic ethanol, and the amount was 4 ml. In the dispersion, 0.16 g of a powder preparation of Bacillus subtilis was mixed.

The number of Bacillus subtilis was about 2 × 10 ⁷ cells / ml.

  The compressed gas was nitrogen, and the aerosol container 2 was filled until the internal pressure became 0.9 MPa. Then, the mildew-proof aerosol apparatus 1 was placed in a bathroom of a general house and sprayed. Immediately before spraying, the mildew-proof aerosol apparatus 1 was not shaken. The results are shown in Table 4.

  Bathrooms A to E in Table 4 are bathrooms in which the contents are actually jetted, and are different bathrooms. The mold occurrence locations 1 and 2 are locations where mold has occurred before the mold prevention treatment, that is, locations where mold is easily generated. In addition, the mold in each bathroom is removed before the use of the mold prevention aerosol apparatus 1.

  In addition, “++” in the table indicates that growth of Bacillus subtilis is observed in a range of about 100%, and “++” indicates growth of Bacillus subtilis in a range of about 90%. “+” Indicates that growth of Bacillus subtilis is observed in a range of about 30%.

  Growth of Bacillus subtilis was observed in all of the bathrooms A to E. Such a result was obtained because the Bacillus subtilis bacteria were stored in an effective state inside the aerosol container 2 and the Bacillus subtilis bacteria were dispersed in the aerosol container 2.

  In addition, no strange smell of bacteria was felt during use. This is because miscellaneous bacteria are sterilized with ethanol.

  About four places (mold generation | occurrence | production location 1, 2, a wall, ceiling) of each bathroom of bathroom AE, the mold generation | occurrence | production situation after one month was investigated after the said process. Table 5 shows the results.

  The initial column of Table 5 indicates immediately after the mold prevention treatment. In the case of no treatment, the detection rate of Bacillus subtilis was naturally 0%. When the antifungal treatment was performed, the detection rate of Bacillus subtilis was 100%. Here, the detection rate of Bacillus subtilis is the ratio of the number of Bacillus subtilis detected from 4 locations of the above five bathrooms A to J, that is, 20 locations, and is shown below. The value calculated by the formula was expressed as a percentage.

Number of detection sites of Bacillus subtilis / 20 sites When antifungal treatment was performed, the detection rate of Bacillus subtilis after 1 month was 75%. Further, the mold occurrence rate after one month was 25% in the case of no treatment, and was only 5% in the case of the mold prevention treatment. The mold generation rate is a percentage calculated by the following formula.

The number of mold occurrence places / 20 places When the above results were expressed in terms of mold inhibition rate, it was 80%. As for the mold inhibition rate, the value calculated by the following formula was expressed as a percentage.

(Generation rate of mold in untreated bathroom-Mold occurrence rate in mold-proof bathroom) / Mold occurrence rate in mold-free bathroom As shown in Table 5, even after a long period of one month has passed since mold-proof treatment It was found that a high mold inhibition rate of 80% was obtained. This is because the amount of Bacillus subtilis attached is sufficient in the early stage of the antifungal treatment, and the residual of Bacillus subtilis is high.

  As described above, the antifungal treatment tool according to the present invention is suitable for treating, for example, a bathroom in a house.

1 Aerosol device for mold prevention 2 Aerosol container 3 Pressure-resistant can 4 Cap 5 Button 6 Nozzle

Claims (7)

An antifungal microorganism that forms a spore under predetermined conditions and grows on the surface of the antifungal object to suppress the growth of other fungi;
A liquid in which the above fungicidal microorganisms are mixed;
An aerosol container containing the above fungicidal microorganisms and liquid;
An inert compressed gas with low chemical reactivity filled in the aerosol container,
An antifungal aerosol device, wherein the antifungal microorganism is sprayed by the compressed gas. In the mold prevention aerosol apparatus of Claim 1,
An aerosol apparatus for mold prevention, wherein the compressed gas is filled in the aerosol container so that the internal pressure of the aerosol container is 0.7 Mpa or more. In the aerosol-proof aerosol apparatus of Claim 1 or 2,
An antifungal aerosol apparatus, wherein the liquid is a dispersion for dispersing antifungal microorganisms in an aerosol container. In the aerosol-proof aerosol apparatus of Claim 3,
The dispersion liquid contains water and a swellable clay mineral that maintains a dispersed state in water, and both are mixed. In the aerosol-proof aerosol apparatus as described in any one of Claim 1 to 4,
The aerosol container is an all-injection type aerosol apparatus configured to inject substantially all of the mold-proof microorganisms in one operation. In the aerosol-proofing aerosol apparatus according to claim 4 or 5,
The dispersion liquid contains at least one of smectite and mica clay mineral as a swellable clay mineral. In the aerosol-proof aerosol apparatus as described in any one of Claim 1 to 6,
The aerosol container contains lower alcohol,
The amount of lower alcohol is set so that it may occupy 20% or less of the total volume of the contents other than the compressed gas contained in the aerosol container.

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