JP2011205989A - Bacterium for reducing soil-borne plant disease and functional compost utilizing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional compost utilizing a bacterium having a pathogenesis reducing effect on all of fusarium wilt, damping-off and root rot of spinach.SOLUTION: A bacterium KS22 strain that grows in a food waste as a main nutrient source, does not have pathogenicity to spinach but has antagonism to pathogen of fusarium wilt, pathogen of damping-off and pathogen of root rot of spinach and belongs to the genus Bacillus is subjected to liquid culture. The liquid culture is mixed with a solid medium containing a plant-based full ripeness compost and the bacterium KS22 strain is acclimatized to produce compost.

Description

  The present invention relates to a microorganism for reducing soil diseases such as plant blight, wilt and strain rot, and functional compost utilizing the microorganism. More specifically, the present invention relates to microorganisms collected from a compost production process and functional compost utilizing the microorganisms, which reduce soil diseases such as spinach blight, wilt and strain rot.

  Spinach in Hiroshima Prefecture is widely cultivated after “Daikon” in terms of the acreage in the area of vegetables. As an important disease of spinach, it is a wilt that causes wilt of the strain. Spinach cultivation is thorough for the purpose of avoiding soil diseases because there are blight that causes symptoms and seedlings disappear and becomes a missing stock, or there is a stock rot that causes seedling withering and constriction and browning of the main root. Cultivation system based on soil disinfection with a pesticide is used.

  However, soil disinfection with agricultural chemicals disrupts the microflora, degrades the soil, increases production costs, environmental problems that contaminate the surrounding soil and water, and needs for consumers 'safety and security, as well as producers' lightening needs There was a problem that it was deviating from.

  Therefore, as a method of reducing plant diseases instead of soil disinfection with pesticides, we tried to select and identify microorganisms that have antagonistic action against pathogenic bacteria of soil diseases, and tried to reduce soil diseases.

  For example, a plant disease control agent or control technology comprising a vegetative cell of a bacterium belonging to the genus Bacillus having an ability to suppress infection or proliferation of phytopathogenic fungi, bacteria or viruses as an active ingredient is disclosed. (For example, refer to Patent Document 1).

  In paragraph [0019] of Patent Document 1, the vegetative cell can be used alone or in combination with an organic powder solid carrier or an adjunct adjuvant as an optional component. In paragraph [0020], vegetative cell is described. There is a description that processing such as ground part spraying and soil mixing is performed.

  In paragraph [0025], there is a description that the pathogenesis of spinach blight, strain rot and wilt disease can be prevented by preventing the growth or transmission of the pathogen in soil.

  In addition, a technique relating to an agricultural and horticultural fungicide composition containing spores of bacteria belonging to Bacillus subtilis that antagonize phytopathogenic fungi and a moisturizer is disclosed (for example, see Patent Document 2).

  And in paragraph [0020] of Patent Document 2, there is a description that the humectant may be used as a humectant in food additives or the like in general such as oligosaccharides, and is applied to paragraph [0029]. There is a description that spinach strain rot fungus Rhizoctonia solani is included as a plant pathogen.

JP 2007-55982 A Japanese Patent No. 3527557

  Invention of patent document 1 sprays the control agent which combined the vegetative cell of the Bacillus genus bacteria alone or the solid support | carrier which is other arbitrary components, and an adjuvant with a spray spray directly on the leaf of a plant, or soil It is mixed to reduce disease.

  However, when liquid is sprayed on the ground such as plant leaves, microorganisms exhibit antibacterial activity and a mitigating effect is recognized. There was a problem that it did not demonstrate and there was almost no sustained reduction effect.

  When mixed with soil in the state of the control agent itself, depending on the components of the soil, there may be a decrease in the number of microorganisms having an antagonistic action and the growth of soil-borne disease-causing fungi. There was a problem.

  In addition, when the microorganism is used alone as a control agent, or when the microorganism is used as a control agent in combination with a solid carrier such as pearlite and an auxiliary agent such as gelatin, there is a problem that the microorganism is difficult to grow after spraying onto plants or soil. there were.

  Since the example which mixed the control agent and soil in patent document 1 was an example in an ice cream cup, the problem that the control effect in the field where the survival of microorganisms and pathogenic bacteria is influenced by soil is not clear was there.

  Moreover, there was no example about the soil disease of spinach, and there existed a problem that it was not clear whether it had the reduction effect with respect to the soil disease of the spinach in a field.

  In the invention of Patent Document 2, there is a description in the paragraph [0033] that the application of the agricultural and horticultural fungicide composition is soil application, but all examples are only applied by spraying. When the composition itself is sprayed on the soil, depending on the components of the soil, the microorganisms having an antagonistic action may be reduced and the soil infectious disease bacteria may grow, and in the examples also, the effect of reducing the disease in the field is clear. There was no problem.

  In addition, when an agricultural and horticultural fungicide composition containing microorganisms and a moisturizing agent is used, when the liquid is sprayed on the above-ground parts such as plant leaves, the microorganisms exhibit antibacterial activity and a reduction effect is recognized. When liquid was sprayed onto the soil, which is the underground part of the plant, there was a problem that the microorganisms did not exert much antibacterial activity, and there was almost no sustained reduction effect.

  In addition, there is a description that spinach soil disease is applied to bacterial wilt in paragraph [0029], but there is no example, and in particular, all pathogens of spinach bacterial rot, strain rot and wilt disease There is a problem that it is not clear whether it has an effect to antagonize and reduce soil disease.

  The present invention has been devised in view of these problems, and antagonizes all of the wilt disease fungus, blight fungus, and strain rot fungi, which are the major pathogens of spinach, among the microorganisms surviving in the compost production process. It is an object of the present invention to provide a compost capable of selecting and identifying microorganisms having an action and capable of continuously growing the microorganisms that reduce the serious disease of the spinach for a long period of time.

  The “KS22 strain” in the present invention means a microorganism of “reception number FERM AP-21194” belonging to the genus Bacillus.

  The invention of the microorganism for reducing plant soil disease according to claim 1 is characterized in that the microorganism for reducing plant soil disease is collected from a composting process of food waste, grown as a main nutrient source, and spinach. It is a microorganism KS22 strain belonging to the genus Bacillus, which has an antagonistic action against pathogenic bacteria of spinach wilt, pathogenic bacteria of blight, and pathogenic bacteria of strains, which are not pathogenic to To do.

  The invention of functional compost utilizing microorganisms that reduce plant soil diseases according to claim 2 is a liquid culture obtained by inoculating the microorganism KS22 strain in a liquid culture solution using food waste as a nutrient source. It is added to a solid medium containing plant-type ripe compost and mixed, and the mixed solid medium is allowed to stand at 20 ° C. to 50 ° C. for 2 to 8 days to produce the microorganism KS22 strain by solid culture. And

  The invention of the functional compost using the microorganisms for reducing the plant soil disease according to claim 3 is the invention according to claim 3, wherein the solid medium is water-absorbing or breathable with respect to 100 parts by weight of the plant-based mature compost. It is a culture medium containing 5 to 15 weight part of the auxiliary material which improves the physical property of this invention.

  In the invention of claim 1, one kind of microorganism having a specific antagonistic action generally suppresses the growth of one specific kind of soil-borne pathogen, and inhibits the growth of a plurality of pathogenic bacteria at a time. In some cases, it is necessary to prepare a plurality of types of microorganisms according to the type of pathogenic bacteria, but the microorganism KS22 strain of the present invention can alleviate all soil infectious diseases such as spinach wilt, blight and rot. There is an effect.

  In addition, the microorganism KS22 strain of the present invention is a fermentation that mixes and composts at least one of sludge generated during food production, residues generated during beverage production such as beer casks and sake lees, and beverages expired such as milk. Since it was collected in the process, it is safe and secure for consumers and has the effect of not causing soil diseases in plants.

  The action of the microorganism KS22 strain of the present invention has the effect of decomposing and detoxifying the crop growth inhibitory substance contained in the organic matter, since it was collected in the fermentation process of composting food waste. is there.

  Since the microorganism KS22 strain of the present invention can use components contained in food waste as nutrients, it is suitable for long-term survival in a state mixed with compost produced from food waste. There is an effect.

  In the invention of claim 2, since the microorganism KS22 strain is inoculated into a solid medium together with a nutrient source of the microorganism KS22 strain and cultivated by acclimatizing to the solid medium, the microorganism KS22 strain is easy to grow and has a long-lasting effect on reducing soil infectious diseases. The effect of doing.

  In addition to the three functions of soil improvement, such as chemical improvement effects such as nutrient replenishment, physical improvement effects such as water retention and breathability, and biological improvement effects such as organic matter decomposition, certain antagonistic bacteria It is a functional compost with the biological improvement effect to reduce the disease in particular because it uses, and grows various microorganisms, but all of the wilt disease, withering disease and strain rot of spinach It has the effect of reducing the disease.

  In general, compost application increases the soil's biological buffering capacity due to an increase in soil organisms and microorganisms, which may reduce the occurrence of soil disease. It may become nourishing and promote the activity of pathogenic bacteria to ease the occurrence of disease. However, the functional compost of the present invention has the effect of reducing any of the diseases of spinach wilt, withering and stock rot.

  The functional compost of the present invention establishes a microorganism having an antagonistic action against all of spinach wilt, bacterial wilt and strain rot, and serves as a nutrient source for the microorganism. An environment that facilitates the survival of microorganisms having a soil is established, and the effect of reducing soil disease is sustained.

  The functional compost of the present invention has an effect of being suitable for organic cultivation because it has an effect of substituting an agrochemical for reducing the three major diseases of spinach.

  The functional compost utilizing the KS22 strain of the present invention has not only the effect of having an antagonistic action, but also not only the five major elements of nitrogen, phosphorus, potassium, calcium, and magnesium that bring about an effect of improving soil chemistry. It also contains trace elements such as zinc, manganese, iron, and boron, and when the organic matter content in the soil increases, soil agglomeration progresses and a relatively large pore is formed, resulting in an effect of improving soil physical properties. This has the effect of improving the root growth environment, such as making the roots of crops easier to grow.

  Since the KS22 strain has the property of growing by pure culture even in a non-sterile medium, a liquid culture obtained by inoculating the microbial strain KS22 into a liquid culture solution using food waste as a nutrient source is added. There is an effect that the solid medium containing the mature ripe compost to be used may be non-sterile.

  The functional compost of the invention of claim 3 has the same effect as that of claim 2. And since the auxiliary material which improves physical properties, such as water absorption and air permeability, is mixed, there exists an effect of increasing microbial activity.

It is a schematic diagram in the case of prevention circle formation. It is a schematic diagram in the case of inhibition spot formation. It is a figure showing the influence which it has on the growth of pH of KS22 strain. It is a figure which shows the molecular phylogenetic tree with the known related microorganisms calculated | required based on 16SrDNA base sequence.

  First, if microorganisms are collected from the composting process of food waste, it is safe for plants, and if they are returned to composting, the microorganisms can grow, and the microorganisms are collected from the composting process. In particular, microorganisms having an antagonistic action against the pathogens of spinach wilt, withering and strain rot were searched.

  The microorganism having an antagonistic action of the present invention is fermented by mixing at least one or more of sludge generated in the food production process, residues generated in the beverage production process such as beer casks and sake lees, and expired beverages such as milk. From each of the deposits in the process of composting, 1 g of 123 samples having different ripening levels were collected.

  Next, microorganisms having an antagonistic action are searched for by the method of confronting the pathogens of spinach wilt, wilt and strain rot.

  About 1 g of the collected sample was immediately weighed into a test tube having a volume of 26 ml, 9 ml of sterilized physiological saline was added, and the mixture was stirred and mixed with a vortex mixer for about 5 minutes. The supernatant of 10% suspension water that was allowed to stand indoors for about 20 minutes was immersed in a circular filter paper 1 (diameter 8 mm, thick, manufactured by Advantech) and then used as a test sample for the antipodal test.

  As the PDA plate medium for the antipodal test, 3.9 g of potato dextrose agar medium (manufactured by Nissui) was dissolved in 100 ml of deionized water, sterilized at 121 ° C. for 15 minutes, and then transferred to a petri dish and transferred to a solidified PDA medium. . All of the PDA media used in the present invention have the same composition as the PDA plate media used in the antipodal test.

  And inoculate spinach pathogen 2 in the center of the PDA plate culture medium, place the soaked circular filter paper 1 at four equally spaced points, and culture in a thermostatic bath at 25 ° C. for 2 days, The formation of blocking circle 3 or blocking plaque 4 and the growth of pathogenic bacteria 2 were observed.

  Here, an antibacterial determination method will be described with reference to FIG. 2 or FIG. If a microorganism group producing an antibacterial substance is present in the supernatant, a growth inhibition circle 3 or a growth inhibition spot 4 of the pathogenic fungus 2 is formed around the circular filter paper 1. In the case of the growth inhibition spots 4, the antibacterial property was determined by measuring the distance 6 between the outer edge of the circular filter paper 1 and the outer edge of the growth zone of the pathogenic bacteria 2.

  In the same manner as the method for observing the formation of the inhibition circle 3 or the inhibition plaque 4 of the pathogen of the spinach blight, the inhibition circle 3 or the inhibition plaque 4 is observed for the pathogen of the spinach wilt and the strain rot. I was able to do it.

  The group of antagonistic bacteria that formed clear inhibition circle 3 or inhibition plaque 4 was purified by repeated streak smear culture. Further, a confrontation test was performed using purified bacteria, and 15 strains that formed clear inhibition circles 3 or inhibition spots 4 were treated as isolates.

  A confrontation test with each pathogenic fungus of spinach blight, strain rot and wilt was carried out for each of the 15 isolates. Tables 1 and 2 show the results of the confrontation test. In Tables 1 and 2, since the inhibition circle 3 is formed in the case of the stock rot fungus, the inhibition circle 3 has a diameter 5 of 10 mm or more as ◯, 5 mm or more and less than 10 mm as △, and in the case of withering or wilt disease bacteria Since the inhibition spots 4 are formed, the distance 6 between the outer edge of the circular filter paper 1 and the growth zone of the pathogen 2 is 10 mm or more, and ◯ is 5 mm or more and less than 10 mm is Δ. The number indicates the strain number temporarily assigned.

  From Table 1 and Table 2, the strains that showed antibacterial activity against the three types of pathogenic bacteria 2 are narrowed down to six types. Among the six types of strains, the size of the inhibition circle 3 against the strain fungus is 21 mm to 32 mm in diameter, and among the six types of strains, the diameter 5 of the inhibition circle 3 of the KS22 strain is 32 mm and is the largest antibacterial activity. Confirmed the strongest.

  Next, the six strains were cultured (30 ° C., 3 days) in a test tube slant medium (PDA medium), and the growth state was observed with the naked eye or using a microscope. The characteristics of the visual observation results are shown in Tables 3 and 4. The color tone and gloss of the strain are all milky white and glossy. In Tables 3 and 4, “surface” indicates that the strain grew on the surface of the agar, and “deep” indicates that it grew to the deep portion of the agar. In addition, A in the “origin” column is a primary fermentation completed product (2 weeks after the start of fermentation), B is a primary fermentation product (within 2 weeks from the start of fermentation), and C is a secondary fermentation product (2 to 2 from the start of fermentation). 4 months later).

  From Table 3, the KS22 strain does not have a sticky substance and does not form a capsule, etc., so the KS22 strain has a property that it can be easily picked with a platinum loop.

  Furthermore, in addition to the visual characteristics and physiological properties shown in Tables 3 and 4, in the process of handling the strain, the growth of the KS22 strain is fast, the formation of a typical crater-like colony, at 80 ° C. The KS22 strain was selected because it was alive and the tough bacteria were easy to handle.

  Next, the pH of the optimal growth conditions for the KS22 strain was tested. First, the KS22 strain was cultured and grown in a test tube slant medium (PDA medium) (30 ° C., 3 days), then agar was removed from the PDA medium, and 20 ml each of the medium from which the agar had been removed was placed in a 100 ml Erlenmeyer flask. Then, after shaking culture at 30 ° C. and 120 rpm for 20 hours in a constant temperature water bath, the growth degree OD660 (absorbance measurement at 660 nm) was carried out while changing the pH value. The result is shown in FIG. FIG. 3 shows that the optimum growth pH of the KS22 strain is 7-9.

  Next, other physiological properties for the KS22 strain are shown in Table 5. In Table 5, + indicates that the material has the properties described in the item.

From Table 5, the KS22 strain was able to grow at 25 ° C. to 50 ° C., was Gram-positive with Neisseria gonorrhoeae, had spore-forming ability, and showed catalase activity and oxidase activity. From these properties, the KS22 strain was estimated to be a bacterium of the genus Bacillus . The KS22 strain has heat resistance because it survives in 80 ° C. compost.

  Next, Tables 6 and 7 show the test results using API50CHB. Positive is represented by + and negative is represented by-.

  Table 6 or Table 7 shows that glycerol, glucose, fructose or the like was fermented, D-arabinose, inulin or raffinose was not fermented, gelatin was hydrolyzed, and nitrate was reduced.

  Further tested physiological properties are shown in Table 8. Positive is represented by + and negative is represented by-.

  From Table 8, KS22 strain grew even under anaerobic conditions, grew at 50 ° C., grew with 10% NaCl, hydrolyzed starch and casein, and showed lipase activity.

Next, 16S ribosomal DNA (16SrDNA) of KS22 strain was sequenced. As a result, as a result of BLAST search of the obtained base sequence with an international base sequence database, it showed a very high homology with the 16S rDNA base sequence of Bacillus bacteria. The homology rate with the reference strain Bacillus amyloliquefaciens NBRC15535 was 99.7%, and the homology rate with Bacillus subtilis IAM12118 was 99.4%. The obtained molecular phylogenetic tree is shown in FIG.

In the molecular phylogenetic tree of FIG. 4, the KS22 strain is closely related to Bacillus amyloliquefaciens , and it is shown that a perfectly matched strain has not been identified.

Furthermore, it can grow under aerobic and anaerobic conditions, it does not oxidize raffinose, it has been separated from the sediment during the compost production process, it can grow well in the aqueous extract of immature compost, and composting microorganisms The malodorous component and germination inhibitory component of lower fatty acids, which are the characteristics of the above, are used as a carbon source, and peptides (molecular weight of about 10,000 Dalton, crop growth inhibitory substance) are used as a nitrogen source, growing in addition to the carbon source and the nitrogen source. It grows well in an artificial medium that requires cobalt ion as an element, is a kind of non-brominated and composted microorganism, and spinach blight, strain rot and wilt disease with only one strain properties or characteristics of KS22 strain that has an antimicrobial effect at the same time with respect to the properties or characteristics and differences Bacillus amyloliquefaciens is KS22 strains from Rukoto was determined to be a new strain.

  Next, the KS22 strain having antagonistic properties against the three important pathogens of spinach is grown.

  The KS22 strain was inoculated into an in vitro slant medium of PDA medium, cultured at 30 ° C. for 2 days, and stored in a cool dark place.

150 g of mature ripe compost (trade name “Toyo” manufactured by Kume Sangyo Co., Ltd.) is suspended in 1 l of water, stirred and mixed for about 5 minutes, filtered through absorbent cotton, and the filtrate is centrifuged (about 5000 × g , 5 minutes), and the supernatant was used as the basal medium of KS22 strain.

  To the basal medium, 1% by weight of waste powdered skim milk was added as food waste to prepare a liquid culture medium. 20 ml each of the liquid culture medium is put into six 100 ml Erlenmeyer flasks, sterilized by autoclave (120 ° C., 15 minutes), cooled, inoculated with one platinum loop of KS22 strain slope culture, and 30 ° C. constant temperature water bath Shaking culture (150 rpm) was performed for 2 days.

  200 ml each of the liquid culture medium is put into 6 500 ml Erlenmeyer flasks, sterilized by autoclave (120 ° C., 15 minutes), and after cooling, the total amount of culture for one 100 ml Erlenmeyer flask is added to one 500 ml Erlenmeyer flask. Each was added, and shaking culture (150 rpm) was performed for 2 days in a 30 ° C. constant temperature water bath with six 500 ml Erlenmeyer flasks.

  Next, functional compost mixed with KS22 strain is produced.

  A wooden box (25cm wide x 25cm deep x 40cm high) with a wire mesh attached to the bottom, 5kg of plant compost (product name: fertility) that is fully matured, and physical properties such as water absorption and breathability As a secondary material to improve the above, 0.5 kg of pearlite was mixed to obtain a solid culture medium. The solid medium was left unsterilized.

  Here, the solid medium may be in a non-sterile state or a sterilized state without being sterilized. Even if the solid medium is non-sterile, the KS22 strain is characterized by being able to grow purely.

  The plant ripe compost (product name: fertility) is made from woody raw materials such as bark (bark), pruned branches, and waste from forest cutting, and is used as a nitrogen source for food industry sludge (milk beverages, confectionery and bakery, This is a fully-ripened compost that has been fermented and matured for 6 months or more by fusing the waste water from the noodle industry, brewing industry, etc. by the activated sludge method), animal and plant residues, waste milk, molasses and the like.

  Of the six 500 ml Erlenmeyer flasks, five (5 liters) were centrifuged (1 l, 5 min) to collect the KS22 strain, and the collected KS22 strains were cultured in the remaining one 500 ml Erlenmeyer flask. I put it in a thing.

Next, in order to acclimate and culture the KS22 strain to a solid, the liquid culture in one 500 ml Erlenmeyer flask collected in the solid medium is inoculated, and at least 20 solid culture medium inoculated with the liquid culture is inoculated. About 5 kg of functional compost was produced by standing at room temperature for 4 days. The functional compost had a pH of about 9 and 9 × 10 ⁹ cfu / g KS22 strain had grown.

  In the functional compost, the KS22 strain is inoculated into a solid medium together with a nutrient source of the KS22 strain and cultivated by acclimatizing the solid, so that the microorganism KS22 strain is easily grown in the functional compost, and the functional compost is used as soil. After mixing, the effect of reducing soil infectious diseases can be prolonged.

  Cultivation tests were conducted for three important diseases of spinach.

First, spinach wilt disease pathogen ( Fusarium oxysporum ), spinach blight pathogen ( Pythium aphanidermatum ), and spinach strain rot pathogen ( Rhizoctonia solani ), respectively, bran: vermiculite (1: 4) medium After cultivation at 25 ° C. for 10 days, commercially available soil (JA soil, manufactured by Hiroshima Fertilizer Co., Ltd., containing 0.2 g nitrogen, 1 g phosphorus, 0.2 g phosphorus, 0.2 g potassium) The functional compost was mixed at a ratio of 80 g.

  8 l per planter (590 mm × 180 mm × 180 mm) of the pathogen-contaminated soil was filled and allowed to stand for 7 days.

  After 7 days, 100 grains of spinach (variety New Anna R4) were sown per pulter. In the house, the soil was irrigated so that it would not become dry soil, and the soil temperature was controlled at a minimum of 20 ° C. so that soil pathogens could easily develop.

  The onset after sowing is summarized in Table 9 for spinach wilt, Table 10 for wilt, and Table 11 for strain rot. In each table, functional compost using plant-based mature compost (trade name Toyoji) is described as “present invention”, and the case where compost utilizing antagonistic bacteria is not mixed is described as “none”. .

  From Table 9, when the functional compost utilizing the microorganism of the present invention is used, the establishment rate is improved by about 1.4 times compared to the case where the compost utilizing the antagonistic bacteria is not used, and the disease incidence is The effect was reduced to about 1/4.

  From Table 10, when the functional compost utilizing the microorganisms of the present invention is used, the seedling establishment rate is substantially the same as when the compost utilizing antagonistic bacteria is not used, but the disease incidence is about The effect of drastically decreasing to 1/14 was observed.

  From Table 11, when the functional compost utilizing the microorganisms of the present invention is used, the establishment rate is improved by about 1.1 times compared to the case where the compost utilizing antagonistic bacteria is not used, and the disease incidence rate Was drastically reduced to about 1/8.

  From Tables 9 to 11, the functional compost utilizing the microorganisms of the present invention is found to have antagonistic properties against pathogens of spinach blight, wilt and strain rot, and spinach blight. It can be seen that the effect of reducing wilt and strain rot is extremely large.

1 Circular filter paper 2 Pathogen 3 Stop circle 4 Stop spot 5 Diameter 6 Distance

Claims (3)

  Microorganisms that reduce plant soil diseases are collected from the composting process of food waste, grow as food nutrients as the main nutrient source, have no pathogenicity to spinach, but pathogens of spinach wilt disease, A microorganism for reducing plant soil diseases characterized by being a microorganism KS22 strain belonging to the genus Bacillus, having an antagonistic action against pathogenic bacteria of bacterial wilt disease and pathogenic fungi of stock rot.   A liquid culture obtained by inoculating the microbial strain KS22 into a liquid culture solution using food waste as a nutrient source is added to and mixed with a solid medium containing plant-type fully-ripened compost, and the mixed solid medium is mixed at 20 ° C. A functional compost utilizing microorganisms that reduce plant soil diseases, wherein the microorganism KS22 strain is produced by solid culture at -50 ° C for 2 to 8 days.   The solid medium is a medium containing a mixture of 5 to 15 parts by weight of an auxiliary material that improves physical properties such as water absorption and air permeability with respect to 100 parts by weight of plant-based mature compost. The functional compost using the microorganisms which reduce the plant soil disease of Claim 3 characterized by these.

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