JP2011110448A - Crushing medium and crushing kit - Google Patents

Crushing medium and crushing kit Download PDF

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JP2011110448A
JP2011110448A JP2009266326A JP2009266326A JP2011110448A JP 2011110448 A JP2011110448 A JP 2011110448A JP 2009266326 A JP2009266326 A JP 2009266326A JP 2009266326 A JP2009266326 A JP 2009266326A JP 2011110448 A JP2011110448 A JP 2011110448A
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Hiroshi Nishida
博 西田
Dai Yonezawa
大 米澤
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a crushing medium capable of crushing a sample effectively although the impact upon the container is small. <P>SOLUTION: The crushing medium 1 has such a structure that two or more annular members 2 are connected together. More specifically, the crushing medium 1 and a target sample are contained together in the container, and the container vibrates to crush the sample. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、試料を破砕するための破砕媒体および当該破砕媒体を用いた破砕キットに関するものである。   The present invention relates to a crushing medium for crushing a sample and a crushing kit using the crushing medium.

従来より、破砕容器に試料と破砕媒体とを収容した後、破砕容器に振動を加えることで、試料を破砕媒体により破砕する破砕装置が知られている。このような破砕装置では、破砕容器の振動により、破砕媒体が試料に衝突する、あるいは破砕媒体が試料を破砕容器の壁面に押し付けることで、試料が破砕される（たとえば、特許文献１参照。）。   2. Description of the Related Art Conventionally, a crushing apparatus that crushes a sample with a crushing medium by applying vibration to the crushing container after accommodating the sample and the crushing medium in a crushing container is known. In such a crushing device, the crushing medium collides with the sample by the vibration of the crushing container, or the crushing medium presses the sample against the wall surface of the crushing container (for example, refer to Patent Document 1). .

特開２００４−２０９３２２号公報（全文、請求項１等）JP 2004-209322 A (the whole sentence, claim 1 etc.)

特許文献１に記載の発明では、破砕媒体として、ガラス、セラミックあるいは金属などから成る微小ビーズを用いている。微小ビーズを破砕媒体に用いて短時間で破砕を進行させる場合には、重量が大きいビーズを用いることでより大きな衝撃を試料に与えることが必要となる。   In the invention described in Patent Document 1, microbeads made of glass, ceramic, metal, or the like are used as the crushing medium. In the case where crushing is advanced in a short time using micro beads as a crushing medium, it is necessary to give a larger impact to the sample by using beads having a large weight.

しかし、重量が大きいビーズを用いる場合には、破砕容器がその衝撃に耐えられず、破砕容器が破損するという問題がある。   However, when using heavy beads, there is a problem that the crushing container cannot withstand the impact and the crushing container is damaged.

そこで、本発明は、容器への衝撃が小さいにも関わらず、短時間で試料を破砕できる破砕媒体およびそれを用いた破砕キットを提供することを目的とする。   Therefore, an object of the present invention is to provide a crushing medium capable of crushing a sample in a short time despite a small impact on the container and a crushing kit using the same.

上記目的を達成するために、本発明の一形態は、２以上の環状部材が連結されている破砕媒体に関する。特に、本発明は、破砕対象である試料と共に容器内部に収容されて、容器が振動することで上記試料を破砕する破砕媒体であって、２以上の環状部材が連結された破砕媒体に関する。   In order to achieve the above object, one embodiment of the present invention relates to a crushing medium in which two or more annular members are connected. In particular, the present invention relates to a crushing medium that is housed inside a container together with a sample to be crushed, and crushes the sample when the container vibrates, in which two or more annular members are connected.

本明細書において「破砕媒体」とは、試料と接触することにより、試料を破砕する機能を有する媒体のことである。破砕媒体は、例えば、破砕対象である試料と共に容器内部に収容されて、容器内において試料と接触することにより上記試料を破砕する破砕媒体であってよく、好ましくは、破砕対象である試料と共に容器内部に収容されて、容器内において試料と共に振動することにより上記試料を破砕する破砕媒体であってもよい。   In this specification, the “crushing medium” is a medium having a function of crushing a sample by contacting the sample. The crushing medium may be, for example, a crushing medium that is accommodated inside the container together with the sample to be crushed, and crushes the sample by contacting the sample in the container. Preferably, the crushing medium is a container together with the sample to be crushed. It may be a crushing medium that is housed inside and crushes the sample by vibrating with the sample in the container.

本明細書において、「環状部材」とは、環状構造を備える部材であれば特に限定はされないが、好ましくは、連結された別の環状部材と、面接触するように構成された環状部材である。また、環状部材は、１以上の異なる大きさの環状部材を含んでいてもよい。   In this specification, the “annular member” is not particularly limited as long as it is a member having an annular structure, but is preferably an annular member configured to be in surface contact with another connected annular member. . The annular member may include one or more different sized annular members.

また、別の本発明の一形態は、２以上の環状部材が連結されている破砕媒体と、その破砕媒体が破砕する対象である試料を収容する容器と、を含む破砕キットに関する。   Another embodiment of the present invention relates to a crushing kit including a crushing medium to which two or more annular members are connected, and a container for storing a sample that is a target to be crushed by the crushing medium.

本発明の破砕媒体によれば、容器への衝撃が小さいにも関わらず、短時間で試料を破砕することができる。   According to the crushing medium of the present invention, the sample can be crushed in a short time despite the small impact on the container.

本発明の第１の実施の形態に係る破砕媒体の斜視図である。It is a perspective view of the crushing medium concerning a 1st embodiment of the present invention. 図１の破砕媒体を、図１のＡ−Ａ線で切断し矢印方向に見た場合の断面図である。It is sectional drawing at the time of cut | disconnecting the crushing medium of FIG. 1 by the AA line of FIG. 1, and seeing in the arrow direction. 本発明の破砕媒体と共に用いる破砕容器の斜視図である。It is a perspective view of the crushing container used with the crushing medium of this invention. 破砕容器を、図３のＢ−Ｂ線にて切断し矢印方向に見た場合の断面図である。It is sectional drawing at the time of cut | disconnecting a crushing container by the BB line of FIG. 3, and seeing in the arrow direction. 図３の破砕容器に破砕媒体を収容した状態を示す斜視図である。It is a perspective view which shows the state which accommodated the crushing medium in the crushing container of FIG. 本実施の形態の変形例に係る環状部材を図１のＡ−Ａ線と同様の線で切断し矢印方向に見た場合の断面図である。It is sectional drawing at the time of cut | disconnecting the cyclic | annular member which concerns on the modification of this Embodiment with the same line as the AA line of FIG. 1, and seeing in the arrow direction. 本実施の形態の他の変形例に係る破砕媒体の斜視図である。It is a perspective view of the crushing medium concerning other modifications of this embodiment.

本発明に係る破砕媒体１の実施の形態の一例について、図面を参照しながら説明する。図１は、本発明の第１の実施の形態に係る破砕媒体１の斜視図である。また、図２は、破砕媒体１を図１のＡ−Ａ線を含む線で矢印方向に切断した場合の切断面を示す断面図である。   An example of an embodiment of the crushing medium 1 according to the present invention will be described with reference to the drawings. FIG. 1 is a perspective view of a crushing medium 1 according to the first embodiment of the present invention. FIG. 2 is a cross-sectional view showing a cut surface when the crushing medium 1 is cut in the direction of the arrow along a line including the line AA in FIG.

破砕媒体１は、破砕媒体１の長さ方向に、２以上の環状部材２が互いに可動に連結されている、いわゆるあずきチェーン状の部材である。具体的には、破砕媒体１は、環状部材２の穴部に、他の環状部材２の一部を通すようにして、環状部材２同士を連結することで、２以上の環状部材２が鎖状に連なった構造を有する。連結された環状部材２同士の連結部分には、その体積が変化しうる間隙Ｓが形成される（図２参照）。また、本実施の形態では、環状部材２は、たとえば、その外周が２．５ｍｍ〜５ｍｍ程度であり、その内周が１．５ｍｍ〜３ｍｍ程度である。また、環状部材２は、ステンレス、炭素、鉛、チタン、タングステン、ジルコニア、タングステンカーバイド、セラミック、ガラスあるいはポリフッ化エチレン等の単体または複合体等、どのような材料から形成されていてもよい。特に、環状部材２を主に金属から構成して成る場合には、安価な破砕媒体１とすることができる。その中でも溶液の種類を選ばない耐薬品性のステンレスが好ましく、たとえば、ステンレスの中でもＳＵＳ３１６を用いるのが好ましい。また、環状部材２の材料は、破砕しようとする試料に応じて適宜決定することができる。   The crushing medium 1 is a so-called chain-shaped member in which two or more annular members 2 are movably connected to each other in the length direction of the crushing medium 1. Specifically, in the crushing medium 1, two or more annular members 2 are chained by connecting the annular members 2 so that a part of the other annular member 2 is passed through the hole of the annular member 2. It has a continuous structure. A gap S in which the volume can be changed is formed in a connecting portion between the connected annular members 2 (see FIG. 2). In the present embodiment, the annular member 2 has, for example, an outer circumference of about 2.5 mm to 5 mm and an inner circumference of about 1.5 mm to 3 mm. Moreover, the annular member 2 may be formed of any material such as stainless steel, carbon, lead, titanium, tungsten, zirconia, tungsten carbide, ceramic, glass, or a single substance or a composite of polyfluorinated ethylene. In particular, when the annular member 2 is mainly composed of metal, the crushing medium 1 can be made inexpensive. Among these, chemical-resistant stainless steel that does not select the type of solution is preferable. For example, SUS316 is preferably used among stainless steel. Moreover, the material of the annular member 2 can be appropriately determined according to the sample to be crushed.

また、本実施の形態では、破砕媒体１を構成する環状部材２は、環が切断されていない輪状部材２ａと、環の一部が切断された連結部材２ｂと、から構成されている。以後、輪状部材２ａおよび連結部材２ｂの両方を指す場合には、「環状部材２」という。連結部材２ｂは、環の一部が切断されているので、輪状部材２ａと容易に連結することができる。なお、本実施の形態においては、破砕媒体１は、輪状部材２ａ同士の間に２つの連結部材２ｂが介在するように連結されている。言い換えると、破砕媒体１は、輪状部材２ａが２つおきに連結されている。   Moreover, in this Embodiment, the cyclic | annular member 2 which comprises the crushing medium 1 is comprised from the ring-shaped member 2a from which the ring is not cut | disconnected, and the connection member 2b from which a part of ring was cut | disconnected. Hereinafter, when referring to both the ring-shaped member 2a and the connecting member 2b, they are referred to as “annular member 2”. Since a part of the ring is cut off, the connecting member 2b can be easily connected to the ring-shaped member 2a. In the present embodiment, the crushing medium 1 is connected so that two connecting members 2b are interposed between the ring-shaped members 2a. In other words, the crushing medium 1 is connected to every two ring-shaped members 2a.

次に、本発明の実施の形態に係る破砕媒体１と共に用いる容器（以後、「破砕容器」という）１１について図面を参照しながら説明する。図３は、破砕容器１１の斜視図である。また、図４は、破砕容器１１を図３のＢ−Ｂ線で切断し、矢印方向に見た場合の断面図である。図５では、破砕容器１１の内部に破砕媒体１を入れた状態を示す斜視図である。   Next, a container (hereinafter referred to as “crushing container”) 11 used with the crushing medium 1 according to the embodiment of the present invention will be described with reference to the drawings. FIG. 3 is a perspective view of the crushing container 11. 4 is a cross-sectional view of the crushing container 11 taken along line BB in FIG. 3 and viewed in the direction of the arrow. In FIG. 5, it is a perspective view which shows the state which put the crushing medium 1 in the inside of the crushing container 11. FIG.

破砕容器１１は、容器本体１２および蓋体１３を主に有する。破砕容器１１は、破砕媒体１、試料の他、好適に補助液等を入れて加振するための容器である。破砕容器１１は、振動する破砕媒体１からの衝撃を受けても耐え得る十分な強度を有していればどのような容器であってもよい。破砕容器１１の材質としては、たとえば、ポリカーボネート、ポリプロピレン、ポリエチレン、ポリスチレンおよびポリフッ化エチレン等の合成樹脂、ステンレス等の金属あるいはガラス等を用いることができる。さらに、容器本体１２の内面に、耐薬品性が高く、かつ摩擦係数が小さいフッ素系樹脂等にてコーティングしたものを用いてもよい。フッ素系樹脂等にて容器本体１２の内面をコーティングした場合には、破砕容器１１に腐食性の緩衝液や抽出液を収容する場合にも用いることができる。   The crushing container 11 mainly has a container body 12 and a lid body 13. The crushing container 11 is a container for suitably adding a supplementary liquid or the like in addition to the crushing medium 1 and the sample. The crushing container 11 may be any container as long as it has sufficient strength to withstand impact from the vibrating crushing medium 1. As a material of the crushing container 11, for example, synthetic resin such as polycarbonate, polypropylene, polyethylene, polystyrene, and polyfluorinated ethylene, metal such as stainless steel, glass, or the like can be used. Further, the inner surface of the container body 12 may be coated with a fluorine resin having a high chemical resistance and a low friction coefficient. When the inner surface of the container main body 12 is coated with a fluorine-based resin or the like, it can also be used when a crushing buffer 11 contains a corrosive buffer or extract.

容器本体１２は、有底円筒状で、略半球状に凹設された底面を有する容器である。容器本体１２の底面は、略半球状に限らず、半楕円球状または円錐状に凹設されていてもよい。また、容器本体１２としては、試料の材質や量に応じて２ｍｌ〜５０ｍｌの容積のものを用いることができる。さらに、容器本体１２が、透明もしくは半透明である場合には、容器本体１２に収容した試料の状態、あるいは破砕の進行状態を確認しながら破砕を行いやすいものとなる。   The container body 12 is a container having a bottomed cylindrical shape and having a bottom surface recessed in a substantially hemispherical shape. The bottom surface of the container body 12 is not limited to a substantially hemispherical shape, and may be recessed in a semi-elliptical spherical shape or a conical shape. Moreover, as the container main body 12, the thing of the volume of 2 ml-50 ml can be used according to the material and quantity of a sample. Furthermore, when the container main body 12 is transparent or translucent, it becomes easy to perform crushing while confirming the state of the sample accommodated in the container main body 12 or the progress of crushing.

蓋体１３は、容器本体１２の開口部を開閉可能に封止する役割を有する。容器本体１２の開口部外周および蓋体１３の内周部にねじが形成されているので、容器本体１２の開口部に、蓋体１３を螺合できるように構成されている。なお、容器本体１２の開口部側に当接する蓋体１３の部分には、ゴム等の弾性体から主に成るパッキン１４が配置されている。したがって、蓋体１３が容器本体１２に螺合されると、破砕容器１１が密閉される。   The lid 13 has a role of sealing the opening of the container body 12 so that the opening can be opened and closed. Since screws are formed on the outer periphery of the opening of the container body 12 and the inner periphery of the lid body 13, the lid body 13 can be screwed into the opening of the container body 12. In addition, a packing 14 mainly made of an elastic body such as rubber is disposed on a portion of the lid body 13 that contacts the opening side of the container body 12. Therefore, when the lid 13 is screwed into the container body 12, the crushing container 11 is sealed.

次に、上記にて説明した破砕媒体１および破砕容器１１を用いて試料を破砕する方法の一例について説明する。   Next, an example of a method for crushing a sample using the crushing medium 1 and the crushing container 11 described above will be described.

まず、容器本体１２に、破砕媒体１、試料および補助液を収容する。試料は、破砕媒体１により破砕可能な試料であれば特に限定されない。好ましくは、動物組織、植物組織、細胞、食品、微生物等の生物由来の試料である。また、試料は、数ｍｍ角の動物組織あるいは植物組織の塊を容器本体１２に入れてもよい。あるいは、破砕容器や試料の種類により、試料をある程度の大きさに予め切断した後、その切断した試料を容器本体１２に入れてもよい。また、試料がしなやかで切断され難い繊維質を含む場合等、試料を破砕し難い場合には、液体窒素浴等により凍結させた試料を用いてもよい。凍結させた試料は、容易に破砕されるため、効率よく試料を破砕できる。なお、冷却した破砕媒体１を用いることにより、破砕の際の発熱を防ぐようにしてもよい。   First, the crushing medium 1, the sample, and the auxiliary liquid are accommodated in the container body 12. The sample is not particularly limited as long as the sample can be crushed by the crushing medium 1. Preferably, it is a sample derived from an organism such as animal tissue, plant tissue, cell, food or microorganism. In addition, as a sample, a lump of animal tissue or plant tissue of several mm square may be put in the container body 12. Alternatively, the sample may be preliminarily cut into a certain size depending on the type of the crushing container or the sample, and then the cut sample may be put into the container body 12. In addition, when it is difficult to crush the sample, such as when the sample contains fibers that are flexible and difficult to cut, a sample frozen in a liquid nitrogen bath or the like may be used. Since the frozen sample is easily crushed, the sample can be crushed efficiently. In addition, you may make it prevent the heat_generation | fever in the case of crushing by using the cooling crushing medium 1 cooled.

補助液としては、水、アルコール、ＰＢＳ、培地等、試料の性質及び破砕の目的に応じて適宜決定することができる。たとえば、試料から核酸又はタンパク質等を抽出する場合には、核酸又はタンパク質を安定的に保持することができる補助液や核酸やタンパク質が溶けやすい補助液を選択するのが好ましい。なお、補助液は、抽出物を安定化する塩等の溶質を含んでいてもよい。   The auxiliary liquid can be appropriately determined according to the properties of the sample and the purpose of crushing, such as water, alcohol, PBS, and culture medium. For example, when nucleic acid or protein is extracted from a sample, it is preferable to select an auxiliary solution that can stably hold the nucleic acid or protein or an auxiliary solution that easily dissolves nucleic acid or protein. The auxiliary liquid may contain a solute such as a salt that stabilizes the extract.

次に、試料と破砕媒体１とを接触させ、破砕媒体１に動きを加える（以下、「破砕工程」という）。破砕工程は、例えば、容器本体１２を、蓋体１３により密閉した状態で行うことができる。また、破砕工程は、試料等を収容した破砕容器１１に振動を加え、破砕容器１１内の試料と破砕媒体１とを攪拌し、破砕容器１１内の試料と破砕媒体１を超音波処理し、又は破砕容器１１内の破砕媒体１のみを振動させることにより行うことができる。破砕容器１１又は破砕媒体１に加える振動としては、破砕容器１１を上下左右に往復運動させてもよいし、あるいは、破砕容器１１を８の字状に旋回させる等、往復運動以外の振動であってもよい。破砕工程における温度は、破砕する試料及び破砕の目的、使用する破砕媒体１の材質に応じて適宜決定することができる。破砕工程の時間も、破砕する試料及び破砕の目的、使用する破砕媒体１の材質に応じて適宜決定することができるが、好ましくは、３秒〜３０分であり、より好ましくは、１分〜１５分である。また、破砕工程は、１回の操作により行ってもよいし、条件その他を変更し又は変更することなく２回以上に分けて行ってもよい。例えば、試料が塊状である組織である場合、当該試料を、破砕媒体１を用いて上記方法により破砕した後、より小さい環状部材からなる破砕媒体１又はビーズ等を用いて更に破砕してもよい。試料として生体由来資料を使用した場合、好ましくは破砕後の試料が、更に分離・抽出操作を行うことにより、各種分析用試料として使用することができる程度まで破砕する。   Next, the sample and the crushing medium 1 are brought into contact with each other to add movement to the crushing medium 1 (hereinafter referred to as “crushing step”). A crushing process can be performed in the state which sealed the container main body 12 with the cover body 13, for example. Further, in the crushing step, vibration is applied to the crushing container 11 containing the sample and the like, the sample in the crushing container 11 and the crushing medium 1 are agitated, and the sample in the crushing container 11 and the crushing medium 1 are subjected to ultrasonic treatment. Or it can carry out by vibrating only the crushing medium 1 in the crushing container 11. The vibration applied to the crushing container 11 or the crushing medium 1 may be a vibration other than the reciprocating movement, such as reciprocating the crushing container 11 up and down, left and right, or turning the crushing container 11 into a figure 8 shape. May be. The temperature in the crushing step can be appropriately determined according to the sample to be crushed, the purpose of crushing, and the material of the crushing medium 1 to be used. Although the time of the crushing process can also be appropriately determined according to the sample to be crushed, the purpose of crushing, and the material of the crushing medium 1 to be used, it is preferably 3 seconds to 30 minutes, more preferably 1 minute to 15 minutes. Moreover, the crushing step may be performed by a single operation, or may be performed in two or more times without changing or changing conditions and the like. For example, when the sample is a massive tissue, the sample may be crushed by the above method using the crushing medium 1, and then further crushed using the crushing medium 1 or beads made of a smaller annular member. . When a biological material is used as a sample, the sample after crushing is preferably crushed to such an extent that it can be used as various analytical samples by further separation and extraction operations.

上述のような破砕媒体１では、効率よく試料を破砕できる。なぜなら、試料は、破砕媒体１との衝突により破砕されるだけではなく、破砕媒体１の環状部材２同士の連結部分の間隙Ｓに挟まるようにして破砕されるからである。つまり、破砕媒体１に動きが加わることで、破砕媒体１の環状部材２同士の連結部分の間隙Ｓが変化するため、間隙Ｓに入り込んだ試料が破砕されてより小片になる。   With the crushing medium 1 as described above, the sample can be crushed efficiently. This is because the sample is not only crushed by the collision with the crushing medium 1 but also crushed so as to be sandwiched between the gaps S of the connecting portions of the annular members 2 of the crushing medium 1. That is, the movement of the crushing medium 1 changes the gap S at the connecting portion between the annular members 2 of the crushing medium 1, so that the sample that has entered the gap S is crushed into smaller pieces.

また、破砕媒体１は、個々の環状部材２の重量が従来の破砕用ビーズの重量より小さい場合であっても、試料が効果的に破砕されるものとなる。したがって、破砕媒体１が容器へ与える衝撃が小さいにも関わらず、試料を短時間で破砕できる。そのため、耐衝撃性があまり高くない樹脂製あるいはガラス製の破砕容器１１を用いても、試料をより効果的に破砕できる。   Moreover, even if the crushing medium 1 is a case where the weight of each cyclic | annular member 2 is smaller than the weight of the conventional bead for crushing, a sample will be crushed effectively. Therefore, the sample can be crushed in a short time even though the impact of the crushing medium 1 on the container is small. Therefore, even if the resin-made or glass-made crushing container 11 whose impact resistance is not so high is used, the sample can be more effectively crushed.

また、図５に示すように、本実施の形態に係る破砕キット１０は、破砕媒体１と破砕容器１１とを少なくとも含む。特に、破砕媒体１と、樹脂製あるいはガラス製の破砕容器１１と、を含む破砕キット１０である場合には、樹脂製あるいはガラス製の破砕容器１１を用いて試料を破砕する場合であっても、破砕容器１１を変形させたり破損させたりすることなく、試料を短時間で破砕できる。   As shown in FIG. 5, the crushing kit 10 according to the present embodiment includes at least a crushing medium 1 and a crushing container 11. In particular, when the crushing kit 10 includes the crushing medium 1 and the crushing container 11 made of resin or glass, even if the sample is crushed using the crushing container 11 made of resin or glass. The sample can be crushed in a short time without deforming or damaging the crushing container 11.

以上、本発明に係る破砕媒体の実施の形態の一例について説明したが、本発明は、上述の実施の形態に何ら限定されることなく、種々変形した形態にて実施可能である。   As mentioned above, although the example of embodiment of the crushing medium which concerns on this invention was demonstrated, this invention is not limited to the above-mentioned embodiment at all, It can implement in the form variously deformed.

たとえば、上述の実施の形態では、具体的な材料および数値を例示したが、その数値や材質に限られることはない。たとえば、環状部材２の外周は、２．５ｍｍ〜５ｍｍ程度であり、その内周は、１．５ｍｍ〜３ｍｍ程度のものとしたが、これは、破砕容器１１の大きさ、試料の種類および抽出目的物により変化しえるものである。   For example, in the above-described embodiment, specific materials and numerical values are exemplified, but the numerical values and materials are not limited thereto. For example, the outer circumference of the annular member 2 is about 2.5 mm to 5 mm, and the inner circumference is about 1.5 mm to 3 mm. It can change depending on the object.

また、上述の実施の形態では、破砕媒体１は、輪状部材２ａ同士が２つの連結部材２ｂを介在させて連結されるものとしているが、このような形態に限らない。破砕媒体１は、連結部材２ｂのみで構成されていてもよい。あるいは、破砕媒体１は、輪状部材２ａ同士が１つの連結部材２ｂを介在させて連結されるものとしてもよい。あるいは、破砕媒体１は、輪状部材２ａ同士が３つ以上の連結部材２ｂを介在させて連結されるものとしてもよいし、不規則的に輪状部材２ａと連結部材２ｂとが連結されているような形態であってもよい。   Moreover, in the above-mentioned embodiment, although the crushing medium 1 shall be connected with the ring-shaped members 2a interposing the two connection members 2b, it is not restricted to such a form. The crushing medium 1 may be composed of only the connecting member 2b. Alternatively, the crushing medium 1 may be configured such that the ring-shaped members 2a are connected to each other with one connecting member 2b interposed therebetween. Alternatively, the crushing medium 1 may be configured such that the annular members 2a are connected to each other with three or more connecting members 2b interposed therebetween, or the annular members 2a and the connecting members 2b are irregularly connected. It may be a form.

また、上述の実施の形態では、連結部材２ｂは、環の一部が切断されたものであるが、このような形態に限らない。たとえば、連結部材２ｂを用いて破砕媒体１を形成した後で、連結部材２ｂのうち、環の切断された部分を融着等により接続することで、連結部材２ｂを切断部分のない輪状の部材としてもよい。   Moreover, in the above-mentioned embodiment, although the connection member 2b has a part of the ring cut, it is not limited to such a form. For example, after forming the crushing medium 1 using the connecting member 2b, the connecting member 2b is connected to the cut portions of the ring by fusion or the like, so that the connecting member 2b is a ring-shaped member having no cutting portion. It is good.

また、破砕媒体１の表面に１又は複数の溝や凸部を形成してもよい。破砕媒体１の表面に溝や凸部が形成されている場合には、破砕されにくい繊維質を含む試料であっても、溝部で、その繊維質が効果的に切断されるものとなる。   In addition, one or a plurality of grooves or protrusions may be formed on the surface of the crushing medium 1. In the case where grooves and protrusions are formed on the surface of the crushing medium 1, even if the sample includes a fiber that is difficult to crush, the fiber is effectively cut at the groove.

図６は、本実施の形態の変形例に係る破砕媒体１ａを、図２と同じ断面で見た場合の断面図である。図２に示される破砕媒体１では、環状部材２の内周面が環状部材２の穴部の貫通方向と略同一の方向に延びる平面とされているのに対して、図６に示す破砕媒体１ａでは、輪状部材２０ａの内周面は、輪状部材２０ａの中心方向へ向かって凸形状となっている。一方、破砕媒体１ａの有する連結部材２ｂは、破砕媒体１が有する連結部材２ｂと同様に、輪状部材２ｂの穴部の貫通方向と略同一の方向に水平な平面とされている。   FIG. 6 is a cross-sectional view of the crushing medium 1a according to the modification of the present embodiment when viewed in the same cross section as FIG. In the crushing medium 1 shown in FIG. 2, the inner peripheral surface of the annular member 2 is a flat surface extending in the substantially same direction as the penetration direction of the hole of the annular member 2, whereas the crushing medium shown in FIG. 6. In 1a, the inner peripheral surface of the ring-shaped member 20a is convex toward the center of the ring-shaped member 20a. On the other hand, like the connecting member 2b of the crushing medium 1, the connecting member 2b of the crushing medium 1a is a flat surface that is horizontal in the same direction as the through direction of the hole of the annular member 2b.

図６に示すように、輪状部材２０の穴部の内周面が、輪状部材２０ａの中心方向へ向かって凸形状となっている場合には、破砕媒体１ａが、より効果的に試料を破砕できるものとなる。なぜなら、輪状部材２０ａの穴部の内周面が、輪状部材２０ａの中心方向へ向かって凸形状となっている場合には、輪状部材２０ａと連結部材２ｂとが近づいた場合に、面接触しやすくなるからである。この結果、輪状部材２０ａと連結部材２ｂとの間隙Ｓに入った試料は、輪状部材２０ａと連結部材２ｂとの間隙Ｓが小さくなるに従い、より効果的につぶされて、より均一に破砕される。   As shown in FIG. 6, when the inner peripheral surface of the hole of the ring-shaped member 20 is convex toward the center of the ring-shaped member 20a, the crushing medium 1a crushes the sample more effectively. It will be possible. This is because when the inner peripheral surface of the hole of the ring-shaped member 20a is convex toward the center of the ring-shaped member 20a, the ring-shaped member 20a and the connecting member 2b come into surface contact when approaching each other. This is because it becomes easier. As a result, the sample that has entered the gap S between the annular member 20a and the connecting member 2b is more effectively crushed and more uniformly crushed as the gap S between the annular member 20a and the connecting member 2b becomes smaller. .

また、破砕媒体１は、環の貫通方向から見た面が平坦な、板状の環状部材２を連ねた、いわゆる「あずきチェーン」と呼ばれる種類の媒体であるが、このような形態に限られない。   Further, the crushing medium 1 is a medium called a “red chain” in which plate-like annular members 2 having a flat surface when viewed from the penetration direction of the ring are connected, but are not limited to such a form. Absent.

図７は、本発明の変形例であって、環状部材２の代わりに、環状部材２の平坦面が略９０度ねじれているような環状部材２０ｃを用いた場合の破砕媒体１ｂを示す斜視図である。   FIG. 7 shows a modification of the present invention, and is a perspective view showing a crushing medium 1b when an annular member 20c in which the flat surface of the annular member 2 is twisted approximately 90 degrees is used instead of the annular member 2. It is.

環状部材２の代わりに、平坦面が、略９０度ねじれているような環状部材２０ｃを用いた場合には、破砕媒体１ｂは、いわゆる、喜平チェーンを形成する。   When the annular member 20c whose flat surface is twisted approximately 90 degrees is used instead of the annular member 2, the crushing medium 1b forms a so-called Kihei chain.

また、本実施の形態の破砕媒体１では、図１等に、同程度の大きさおよび形状を有する環状部材２が鎖状に連なっているものとされているが、このような形態に限らない。互いに異なる大きさの環状部材２が、鎖状に連なるような形態としてもよい。互いに異なる大きさの環状部材２が鎖状に連なった破砕媒体では、それぞれの間隙Ｓの大きさも異なるため、様々な大きさの試料を破砕できる破砕媒体となる。また、破砕媒体１を構成する環状部材２のうち１つのみ、あるいは２以上の環状部材２のみが、他の環状部材２と異なる大きさあるいは形状とされてもよい。   Moreover, in the crushing medium 1 of this Embodiment, although the cyclic | annular member 2 which has comparable magnitude | size and a shape is made into the chain form in FIG. 1 etc., it is not restricted to such a form. . It is good also as a form that the cyclic | annular member 2 of a mutually different magnitude | size continues in a chain form. In the crushing medium in which the annular members 2 having different sizes are connected in a chain, the sizes of the gaps S are different, so that the crushing medium can crush samples of various sizes. Further, only one of the annular members 2 constituting the crushing medium 1 or only two or more annular members 2 may be different in size or shape from the other annular members 2.

本発明は、たとえば、試料を破砕するために用いることができる。一例を挙げると、本発明の破砕媒体は、試料を破砕することを含む物質の抽出方法に使用することができる。また、さらなる例を挙げると、本発明の破砕媒体は、生体試料を破砕することによる、生体内物質（例えば、タンパク質、又はＤＮＡ若しくはＲＮＡ等の核酸）の抽出において使用することができる。   The present invention can be used, for example, to crush a sample. As an example, the crushing medium of the present invention can be used in a method for extracting a substance including crushing a sample. As a further example, the crushing medium of the present invention can be used in the extraction of in-vivo substances (for example, proteins, or nucleic acids such as DNA or RNA) by crushing a biological sample.

１，１ａ，１ｂ 破砕媒体
２，２０ 環状部材
１０ 破砕キット
１１ 容器（破砕容器） 1, 1a, 1b Crushing medium 2,20 Annular member 10 Crushing kit 11 Container (crushing container)

Claims (4)

２以上の環状部材が互いに可動できるように連結されていることを特徴とする破砕媒体。   A crushing medium, wherein two or more annular members are connected to each other so as to be movable. 請求項１に記載の破砕媒体であって、
前記環状部材は、連結された別の環状部材と、面で接触できるように構成されたことを特徴とする破砕媒体。 The crushing medium according to claim 1,
The crushing medium, wherein the annular member is configured to come into contact with another connected annular member on a surface. 請求項１または請求項２に記載の破砕媒体であって、
前記破砕媒体は、１以上の異なる大きさの環状部材が含まれていることを特徴とする破砕媒体。 The crushing medium according to claim 1 or 2,
The crushing medium, wherein the crushing medium includes one or more annular members having different sizes. 請求項１〜３のいずれか１項に記載の破砕媒体と、
当該破砕媒体が破砕する対象である試料を収容する容器と、を含むことを特徴とする破砕キット。 The crushing medium according to any one of claims 1 to 3,
A crushing kit comprising: a container for storing a sample which is an object to be crushed by the crushing medium.
JP2009266326A 2009-11-24 2009-11-24 Crushing medium and crushing kit Pending JP2011110448A (en)

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