JP2011078386A - Plant culture apparatus and culture vessel used for the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a plant culture apparatus achieving a random movement by floating and free fall or rotary motion close to a very small gravity state which is essentially expected, disturbing recognition in the gravity direction for plant cells, promoting redifferentiation of shoot primordia, and efficiently preparing the plurality of shoot primordia. <P>SOLUTION: The plant culture apparatus includes: a rotating means 3 for rotating a plurality of culture vessels 2 with the shaft as the center; and a tilting means 4 for vertically tilting the culture vessels 2 within a prescribed angle range. Each culture vessel 2 is composed of a bottomed tube, and provided with a plurality of protruding ridges protruding from the peripheral wall inner surface to the inside, and extending to the bottom. Rotation and tilting are provided to the culture vessels 2 with the rotating means 3 and tilting means 4 to thereby disturb the recognition in the gravity direction in the plant cells. Thereby, the cells at the growth point can be redifferentiated in a plurality of directions to efficiently prepare the shoot primordia. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a plant culturing apparatus that is optimal for culturing plant cells made of plant stems in a culture vessel and producing small clusters of meristems called shoot primordia.

  A shoot primordia method is known in which a dome-shaped shoot apical plant cell is cut out in a length of about 1 mm, cultured in a specific medium solution under visible light to obtain a shoot primordium, and the plant is regenerated from its growth point. Since the shoot primordia obtained by this culture are genetically stable and have a large growth capacity, they are used for regeneration of various plants in various fields such as forestry and agriculture (see Patent Document 1). ). Conventionally, as this type of culture device, a shelf type (stationary type), a vibration type and a rotation type device are known. A shelf-type device is a device in which a culture vessel is placed in a stationary state on a shelf for culturing. A vibration-type device places a culture vessel made of an Erlenmeyer flask on an upper plate, and the plate is placed in the left-right direction. Alternatively, a vibration motor that vibrates in the figure eight direction is provided, and by oscillating the culture vessel, a larger amount of oxygen is taken into the culture medium inside to promote differentiation of the growth points of plant cells. However, with the shelf-type and vibration-type devices, the regeneration rate of the obtained shoot primordia varies widely, and there is a limit to making it stably and in large quantities and cheap on a commercial basis.

  On the other hand, a rotating culture apparatus has a light-transmitting acrylic rotating disk installed in a vertical direction so that its axis is substantially horizontal or 15 degrees upward, and a plurality of mounting holes are provided on the disk. , By inserting a culture tube consisting of a cylindrical test tube into each mounting hole and rotating the rotating disk, the gravity direction of the plant cells in the culture tube is disturbed (not subjected to gravity stimulation), With regard to the differentiation of the growth point, it is intended to differentiate the shoot that comes out in the direction opposite to the gravity direction into a plurality of directions. However, such a rotation type device is inserted into the mounting hole of the disk in a substantially lateral state and slowly rotates 360 degrees integrally with the rotation disk, but the culture tube is a test tube having a circular cross section. In addition, since the inner culture solution has a specific gravity of approximately 1 and low viscosity, the plant cells inside move slightly on the inner surface of the circular curved surface in the direction of rotation even if the culture tube rotates, It slides back to the original bottom, and there is little movement such as floating and rotation during that time, and the posture of landing almost directly below the culture vessel is less diverse and stable. Therefore, the culture requires a lot of time.

  Therefore, even in a rotary type culture apparatus, the average number of shoot primordia obtained is 5-10 on average per month, which is not sufficient for industrial scale culture, and the plant cells placed in one culture tube are usually In order to obtain a large amount of shoot primordia, it is necessary to have a large disk that can be equipped with a large number of culture tubes. This increases the load on the drive motor that rotates the tube and increases the number of culture tubes. The work of installing on the disk becomes complicated, and further, it becomes difficult to balance the gravity of the culture apparatus itself, which causes various problems in terms of stability and strength. Therefore, in reality, the maximum number of culture tubes that can be installed is about 200, and the maximum number of shoot primordia obtained in one culture is about 2000 per month.

  Similarly, a culture apparatus that rotates two or more axes has been proposed as a culture apparatus that disturbs the gravity direction of plant cells (see, for example, Patent Documents 2 and 3), but the structure becomes complicated and the cost increases. Inevitable. In addition, the actual shape of plant cells is not a true sphere, but is a complicated shape such as a 16 pyramid or 18 pyramid, and tends to be in a stable posture at the bottom of the culture vessel, giving diversity to its landing posture. Was difficult.

JP-A-9-308401 Japanese Patent Publication No. 7-89798 Japanese Patent Laid-Open No. 2003-9852

  Therefore, in view of the above situation, the present invention intends to solve the problem by realizing a random movement by floating / free fall or rotational movement close to the originally expected microgravity state, with respect to the plant cell in the direction of gravity. The object of the present invention is to provide a plant culturing apparatus capable of efficiently producing a number of shoot primordia by disturbing recognition and improving the regeneration rate of growth points.

  In order to solve the above-mentioned problem, the present invention is a culture vessel comprising a bottomed tube, and a single or a plurality of ridges protruding inward from the inner surface of the peripheral wall and extending to the bottom, along the circumferential direction. And a rotation means for rotating the culture vessel about its axis, and a tilting means for tilting the culture vessel in the vertical direction within a predetermined angular range. 1).

  Here, the rotating means comprises a plurality of rotating rollers for rotating the culture vessel in between, and a driving means for rotating the rotating rollers in the same direction in a synchronized state, and the tilting means And a crank mechanism that tilts the support frame supporting the plurality of rotating rollers up and down within a predetermined angular range, for example, 10 ° up and down about a certain angular position and 20 ° up and down. An apparatus is preferred (claim 2).

  In addition, the culture container has an opening at the upper end, and a plurality of the culture containers are stacked by inserting one bottom part from the other opening part and placing it on the upper end part of the other protruding part. What can be used in a state is preferable (Claim 3).

  In particular, a cylindrical annular piece is provided on the outer periphery of the bottom of the culture vessel, and a concave groove that engages with the annular piece of another culture vessel inserted from the opening is provided at the upper end of the ridge. It is preferable that the one bottom portion is placed on the upper end portion of the other protruding portion in the engaged state (claim 4).

  Further, the present invention is a culture container used for the above-described plant culturing apparatus, comprising a bottomed tube having an opening at the upper end, and protruding from the inner surface of the peripheral wall inward and extending to the bottom, A single or a plurality of the culture vessels can be provided along the direction, and a plurality of the culture vessels can be used in a stacking state by inserting one bottom part from the other opening part and placing it on the upper end part of the other ridge part. A culture vessel is also provided (Claim 5).

  More specifically, a container formed by projecting a total of three protrusions at symmetrical positions 120 degrees apart from the axial center on the inner surface of the peripheral wall is preferable (Claim 6).

  Also, a cylindrical annular piece is provided on the outer periphery of the bottom, and a concave groove is provided at the upper end of the protrusion to engage the annular piece of another culture vessel inserted from the opening. (Claim 7).

  In particular, it is preferable that the bottom portion is formed as a partially convex partial spherical surface, and the annular piece of the outer peripheral portion is set to a length extending downward from the lowest position of the partial spherical shape ( Claim 8).

  Further, it is preferable that the protruding portion is a plate-like fin (rib) which is integrally formed with the container main body of a synthetic resin material and protrudes toward the center of the shaft.

  According to the plant culturing apparatus according to the first aspect of the present invention, the plant cell in the culture solution is lifted by the protrusions as the culture vessel rotates, and the movement, suspension and irregularity of the plant cell inside are accordingly accompanied. Rotation is promoted, and the tilt direction of the culture vessel changes, so the culture solution performs tidal movement while rotating slightly, increasing the movement distance of plant cells, and promoting rotation in an unspecified direction to promote landing posture. Will also be random. Then, by further rotating, the plant cells slide down in the culture solution along the wall surface of the ridge, and in that case, the change in the inclination direction is also added, and when falling, it falls while rotating in a complicated manner. It becomes an irregular movement due to the microgravity state. Therefore, the growth point cells can be redifferentiated in multiple directions by unnaturally disturbing the gravitational direction perceived by the plant, and the shoot primordia can be efficiently produced.

  According to the plant culturing apparatus according to claim 2, the rotating means comprises a plurality of rotating rollers for rotating the culture container in between, and a driving means for rotating the rotating rollers in the same direction in a synchronized state. Since the tilting means comprises a crank mechanism that tilts the support frame supporting the plurality of rotating rollers in a vertical direction within a predetermined angle range, the culture tube is inserted into the rotating disk as in the conventional case. Compared to conventional devices, it is easy to install the culture vessel between the rollers, which improves workability and keeps the device compact even if the number of culture vessels is increased compared to conventional rotary devices. It is. Further, in the conventional rotary type apparatus, there is a problem that the apparatus becomes unstable when the disk is enlarged, but in the present invention, such a concern does not occur, and a stable apparatus configuration can be obtained.

  According to the plant culturing apparatus of the third aspect, it is possible to mount a plurality of culture containers on the gap between the rotating rollers in a state of being stacked in a row, and it becomes easy to improve productivity.

  According to the plant culturing apparatus according to claim 4, the stacking state of the culture vessel is stabilized by engagement, no flapping occurs between the vessels during rotation, and a gap between the vessels is ensured in a reliable and stable posture. It is also possible to supply oxygen smoothly into the container.

  According to the culture container which concerns on Claim 5, a shoot primordia can be efficiently produced in large quantities using the plant culture apparatus of this invention.

  According to the culture container of claim 6, the irregular movement of the internal plant cells can be surely promoted by a total of three symmetrical ridges, and the landing posture can be made random while freely moving when falling. A microgravity state can be created.

  According to the culture container of claim 7, when a plurality of containers are stacked and mounted in a stacking state, the posture is stabilized by the engagement of the annular piece and the concave groove, and no flapping occurs between the containers during rotation. In addition, it is possible to ensure a gap between the containers in a reliable and stable posture, and it is possible to stack many culture containers in a row.

  According to the culture container according to claim 8, since the bottom portion is configured to have a downwardly convex partial spherical shape, the plant cell does not hurt by hitting the corner portion or the like even if the plant cell moves due to dropping or rotation, and the quality of the shoot primordia is improved. Can be reliably maintained. In addition, since the bottom is round as described above, it is unstable when handling the culture vessel alone, but the length of the annular piece for engagement at the outer periphery extending downward from the position of the lowermost part of the partial spherical shape is extended. Therefore, while the conventional culture tube requires a dedicated stand, it can be stably placed on a table or the like by the annular piece and workability is improved.

  According to the culture container of the ninth aspect, since the protrusion is integrally formed with the container main body by a synthetic resin material and is a plate-like fin protruding toward the center of the shaft, the container can be efficiently manufactured and lightweight. Can be.

  Next, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

  FIG. 1 is an explanatory diagram showing the overall configuration of a plant culture apparatus according to the present invention, in which FIGS. 1 to 5 show typical embodiments, in which 1 is a plant culture apparatus, 2 is a rotating disk, 3 is Culture containers 4 and 4 each show a crank mechanism.

  As shown in FIG. 1, the plant culture apparatus 1 includes a plurality of culture containers 2, rotation means 3 that rotates these culture containers 2 about the axis, and tilting that causes the culture containers 2 to tilt up and down within a predetermined angular range. Means 4 are provided. Each culture vessel 2 is formed of a bottomed tube body, and as shown in FIG. 2, a plurality of protrusions 5 that protrude inward from the inner peripheral surface 21 a and extend to the bottom 22 are provided. Each culture container 2 contains plant cells together with the culture solution. By rotating and tilting the culture container 2 by the rotation means 3 and the tilting means 4, the recognition of the direction of gravity in the plant cells is disturbed, and the growth occurs. Many points can appear. The present invention can be applied to all plants that can be regenerated by the shoot primordia method.

  As shown in FIGS. 4 and 5, the rotating means 3 rotates in the same direction with a plurality of rotating rollers 30 that make a pair for rotating the culture vessel 2 between them and the rotating rollers 30 in a synchronized state. And driving means 31 to be driven. In this example, the driving means 31 includes a worm shaft 34 that is belt-driven by a drive motor M1 installed on a base 33, and a worm gear drive that includes a worm wheel 35 that rotates the shaft of the rotating roller 30 by the worm shaft 34. However, the present invention is not limited to this, and it is interlocked by other gear drives that link the rotary rollers 30, other belt drives, for example, using a caterpillar-shaped timing belt, chain drive, etc. Various things can be adopted. In the case of belt drive or chain drive, it is not limited to one that is interlocked only at one end side of each rotating roller as in this example, but between adjacent rotating rollers is alternately interlocked at one end side and the other end side, and one rotation All may be driven synchronously by driving only the rollers. In the present example, the worm shaft 34 is provided with the worm 34 a substantially over the entire length, but the worm may be provided intermittently corresponding to each worm wheel 35.

  A plurality of rotating rollers 30 are arranged in parallel by a frame-shaped support frame 32, and the support frame 32 rotatably supports both ends of each roller. In this example, three or more rotating rollers 30 are provided and two or more culture vessels 2 can be mounted. However, only two rollers may be provided. In addition, the length of the rotating roller 30 is long enough to mount a plurality of culture vessels 2, for example, about 10 in a stacking state. Of course, it is also possible to make it long enough to be connected to 10 or more depending on the scale. Furthermore, in this example, the structure is such that flanges are provided at both ends of the rotating roller 30 and the bottom portion of the lowermost culture vessel placed between the rotating rollers is supported by the lower flange in an inclined state. Instead of the flange, a support rod that supports the center of the bottom of the culture vessel may be provided between the rotating rollers of the support frame 32.

  The support frame 32 is pivotally supported by the base 33 with the worm shaft 34 as an axis, and is supported by the tilting means 4 so as to be reciprocally tiltable up and down within a range of about 20 °. Of course, it is possible to use a worm shaft 34 that is supported at the middle portion, in addition to the worm shaft 34 pivotally supported at the end portion. In addition, the angle range of tilting is not particularly limited, but in this example, tilting is performed within a range in which the opening side is on the upper side so that a culture solution or the like does not leak from the opening of the culture vessel. The tilting means 4 is composed of a crank mechanism 40. The crank mechanism 40 belt-drives the crank wheel 41 by a drive motor M <b> 2 installed on the base 33, and a support arm 42 extending from the crank wheel 41 is pivotally supported on the support frame 32. As a result, as shown in FIGS. 6 and 7, the plurality of rotating rollers 30 can be tilted up and down within a predetermined angle range while maintaining the relative positional relationship with each other. Instead, it is a three-dimensional movement.

  In other words, the culture vessel 2 is swung up and down at the same time while rotating slowly, and the plant cells inside rotate in multiple directions in the culture solution and repeat the movement of free falling in an irregular posture. The tilting means 4 can be realized by other mechanisms such as a cam mechanism in addition to the crank mechanism 40. In this example, the drive motor M2 of the tilting means 4 is a motor different from the drive motor M1 of the rotating means 3 described above, and it can be easily adjusted as appropriate so that the rotation and tilt of the culture vessel are synchronized with each other at the optimum rotation speed. Of course, it is possible to use a common motor. Further, the drive motor may be fixed to the support frame 32 side, and the support arm may be extended from the crank shaft of the drive motor and pivoted to the base 33 side. In this case, the drive motor can also be used as a drive motor for the rotation means. The drive roller is tilted by a support arm extending from the crankshaft of the crankshaft and the rotation roller is driven to rotate by a belt extending from the crankshaft motor central axis. This can be achieved.

  The movement of the rotating means 3 and the tilting means 4 synchronizes optimally the force vector in the rotation direction by the rotating means 3 and the force vector in the up-and-down tilt direction by the tilting means 4 so that the two force vectors do not cancel each other. The culture medium in the vessel is set to repeat the appropriate tidal movement. In this example, as shown in FIGS. 6 and 7, a frame frame 36 is provided on the upper side of the support frame 32 so as to cover the rotating roller 30 and the entire culture vessel 2 placed thereon, A sheet 37 such as a transparent vinyl sheet is placed on the outside of the frame frame 36 so that dust or the like does not enter the culture vessel 2, and an illumination device 38 such as a fluorescent lamp or an LED lamp is provided inside the frame frame. Is provided.

  As shown in FIG. 2, the culture vessel 2 is composed of a bottomed tube body having an opening 20 at the upper end, and the cross-sectional shape from the opening 20 to the bottom is the same spherical surface as the bottom of a conventional culture tube (test tube). The shape is round. The peripheral wall inner surface 21a is provided with one or a plurality of protrusions 5 extending inward and extending to the bottom 22 along the circumferential direction. In this example, in order to further increase the moving distance of the cells, a total of three protrusions 5 are respectively provided at symmetrical positions spaced 120 degrees from the axial center of the peripheral wall inner surface 21a. The material of the culture vessel 2 may be made of a synthetic resin such as polycarbonate resin or a light transmissive material such as glass. In this example, it is made of a light-transmitting synthetic resin, and the protrusion 5 is formed integrally with the container body by a synthetic resin material and is a plate-like fin protruding toward the center of the shaft. For example, as shown in FIG. 9, the protrusion 5 may be formed as a shape in which the peripheral wall is bent inward as shown in FIG. 9, and various other forms are possible.

  When the culture vessel 2 rotates, the plant cells are lifted by the ridges 5 to a certain angle (almost 11 o'clock direction), and when the angle is exceeded, the plant cells move downward in the culture solution from the ridges 5. Free fall. Due to the structure of the device placed on the rotating roller 30, the culture vessel 2 can be set relatively freely in size (volume) as compared to the culture tube inserted in the conventional rotating disk, and two or more, for example, It is also possible to culture with 10 plant cells.

  The bottom portion 22 of the culture vessel 2 is formed in a partially convex partial spherical shape, and an annular piece 23 having a length extending downward from the lowermost portion of the partial spherical shape is projected in a cylindrical shape on the outer peripheral portion thereof. ing. Further, the upper end portion 5a of the ridge portion 5 is provided with a concave groove 5b that engages with the annular piece 23 of another culture vessel 2 inserted from the opening portion 20, as shown in FIG. By inserting the bottom 22 of the culture vessel 2 through the other opening 20 and placing it on the upper end 5a of the other protrusion 5, the annular piece 23 is engaged with the groove 5b. It is configured to be usable in a stacking state with a stable posture. By connecting a plurality of culture vessels 2 in this manner, the culture can be efficiently performed and at the same time, the supply of oxygen is ensured.

  The culture container 2 is dimensioned so that a gap for air circulation is maintained between the opening 20 and the upper container in the stacking state, and the gap moves even by rotation in the stable engagement posture. However, it is possible to cope with the problem by providing an opening hole in the side wall near the opening 20. Further, as shown in FIG. 8, it is of course possible to omit the annular piece and the groove.

  In this example, for example, the culture vessel 2 is rotated at a speed of about 2 rotations per minute by the rotating roller 30 and at the same time, 20 ° in total 10 ° up and down from a position inclined about 15 ° upward along with the rotating roller 30. These ranges are tilted at a speed of about 2 reciprocations per minute, and the rotation / tilting speed is appropriately set according to the type of plant cell. The speed of rotation and vertical tilting is appropriately set according to the type of plant cell.

  FIG. 10 is an explanatory view showing a state of the inside of the culture container 2 during operation of the plant culture apparatus 1 in a sectional view of the container. The direction of rotation is clockwise. At the rotational positions (a) to (b), the plant cells in the culture solution move on the peripheral wall inner surface 21a (inner peripheral surface) between the protrusions 5, but even if they move in the rotational direction, they are 6 directly below by gravity. Repeat the movement back to the hour position. In the drawing, no tilting action appears, but in actuality it moves in an oblique direction deviating from the rotational direction.

  At positions (b) to (c), the protrusion 5 comes to a position immediately below, and the plant cell is lifted up in the culture solution in the rotation direction. Plant cells gather at the corner between the inner peripheral wall surface 21a in the container 2 and the side wall 5c of the ridge 5 and float to promote disturbance of recognition in the direction of gravity.

  At the positions (c) to (d), the plant cell starts to land on the side wall 5c of the protrusion 5 and move. There is a high possibility that the landing posture is different from the initial position of the container 2 on the peripheral wall inner surface 21a. Thereafter, plant cells move obliquely toward the tip side on the side wall 5c of the ridge 5, and begin to fall downward in the culture solution. Since rotation is added in the tilt direction simultaneously with rotation in the rotation direction, a complicated multi-axis rotation drop occurs during the fall.

  At the positions (d) to (e), the landing is made again on the inner surface 21a of the peripheral wall of the container 2. As a result of the complicated rotation and fall, the landing posture has a high randomness that is different from the initial posture. Disturbance occurs. Thereafter, by repeating (a) to (e), the plant cell repeats floating, rotating, and moving, and repeats movement in a state close to microgravity. In the conventional culture device, the plant cells that have been sliding down with a slight rotational motion in a stable posture at the bottom of the culture vessel can reliably perturb the microgravity according to the device of this example. is there.

  According to the present inventor's confirmation by culturing experiments using the shoot apex cells of nanyo abragi (Jatropha), there is 1 shoot primordium formed by culturing the above cells with a conventional rotary type apparatus comprising a disc. Compared to 10 cells per month, according to the apparatus using the container of the present invention, 20 shoot primordia can be redifferentiated in 2 weeks. Compared to four times the number. For example, 10 culture vessels are placed between rollers in a stacking state, and this is mounted in 10 rows by 11 rotating rollers 30, that is, a total of 100 culture vessels are mounted and one culture vessel 2 is mounted. Putting 10 plant cells, the number of shoot primordia produced is about 40,000 per month. It is also possible to place the plant culture device 1 on the plant culture device 1 in two to three or more stages, and a large amount of culture is possible in a space-saving manner.

  Although the embodiments of the present invention have been described above, the present invention is not limited to these embodiments, and can of course be implemented in various forms without departing from the gist of the present invention.

The perspective view which shows the whole structure of the plant culture apparatus which concerns on typical embodiment of this invention. Explanatory drawing which similarly shows the culture container mounted in a plant culture apparatus. Similarly, the longitudinal cross-sectional view which shows a mode that the culture container was made into the stacking state. The top view of a plant culture apparatus similarly. Explanatory drawing of the principal part of a plant culture apparatus similarly. The side view of a plant culture apparatus similarly. Similarly side view. Explanatory drawing which shows the modification of a culture container. Explanatory drawing which shows the other modification of a culture container. Explanatory drawing which shows the mode inside the culture container at the time of operation | movement.

DESCRIPTION OF SYMBOLS 1 Plant culture apparatus 2 Culture container 3 Rotating means 4 Tilt means 5 Projection part 5a Upper end part 5b Concave groove 5c Side wall 20 Opening part 21a Inner surface 22 Bottom part 23 Annular piece 30 Rotating roller 31 Driving means 32 Support frame 33 Base 34 Warm shaft 34a Worm 35 Worm wheel 36 Frame frame 37 Seat 38 Illuminating device 40 Crank mechanism 41 Crank wheel 42 Support arm M1 drive motor M2 drive motor

Claims (9)

A culture vessel comprising a tube with a bottom, projecting inward from the inner surface of the peripheral wall and extending to the bottom, and a single or a plurality of ridges extending along the circumferential direction,
A rotating means for rotating the culture vessel about its axis;
Tilting means for tilting the culture vessel in a vertical direction within a predetermined angle range;
A plant culturing apparatus comprising:   The rotating means includes a plurality of rotating rollers for rotating the culture vessel in between, and a driving means for rotating the rotating rollers in the same direction in a synchronized state, and the tilting means is the plurality of rotating rollers. The plant culturing apparatus according to claim 1, comprising a crank mechanism that tilts a support frame that supports the frame vertically in a predetermined angle range.   In the stacking state, the culture vessel has an opening at the upper end, and the plurality of culture vessels are placed on the upper end of the other protruding portion by inserting one bottom portion from the other opening portion. The plant culture apparatus according to claim 1 or 2, which can be used.   A cylindrical annular piece is provided on the outer periphery of the bottom of the culture vessel, and an upper groove of the ridge is provided with a groove that engages with the annular piece of another culture vessel inserted from the opening, The plant culture apparatus according to claim 3, wherein the one bottom portion is placed on the upper end portion of the other protrusion in the engaged state. A culture vessel used in the plant culture apparatus according to any one of claims 1 to 4,
Consisting of a bottomed tube with an opening at the top,
Protruding ridges projecting inward from the inner surface of the peripheral wall and extending to the bottom are provided in the circumferential direction as a single or a plurality,
A culture vessel that can be used in a stacking state by inserting a plurality of the culture vessels into the upper end of the other protruding portion by inserting one bottom portion from the other opening.   The culture container according to claim 5, wherein a total of three protrusions are provided in a projecting manner at symmetrical positions separated from each other by 120 degrees with respect to the axial center on the inner surface of the peripheral wall.   6. A cylindrical annular piece is provided on the outer periphery of the bottom, and a concave groove is provided at the upper end of the ridge to engage the annular piece of another culture vessel inserted from the opening. Or the culture container of 6.   8. The culture according to claim 7, wherein the bottom part is formed in a partially convex partial spherical shape, and the annular piece of the outer peripheral part is set to a length extending downward from the lowest position of the partial spherical shape. container.   The culture container according to any one of claims 5 to 8, wherein the protrusion is a plate-like fin that is integrally formed with a container body of a synthetic resin material and protrudes toward an axial center.

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