JP2011062131A - Microorganism concentration regulator - Google Patents

Microorganism concentration regulator Download PDF

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JP2011062131A
JP2011062131A JP2009215360A JP2009215360A JP2011062131A JP 2011062131 A JP2011062131 A JP 2011062131A JP 2009215360 A JP2009215360 A JP 2009215360A JP 2009215360 A JP2009215360 A JP 2009215360A JP 2011062131 A JP2011062131 A JP 2011062131A
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microorganism concentration
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JP5471228B2 (en
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Shinichi Yasui
伸一 安井
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Panasonic Corp
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a microorganism concentration-regulator capable of providing a culture liquid having a required microorganism concentration. <P>SOLUTION: The microorganism concentration-regulator is equipped with a microorganism concentration-regulating tool 1, a culture liquid container 5 and a cleaning liquid container 7 selectively connected to the upstream side of the microorganism concentration-regulating tool 1, and a culture liquid-storing container 11 connected to the downstream side of the microorganism concentration-regulating tool 1. The microorganism concentration-regulating tool 1 has a plurality of microorganism-capturing electrode pairs which have at least one of different gaps between the facing electrodes and the different sizes of the facing electrodes, and a voltage-applying means connected to a plurality of the microorganism-capturing electrode pairs respectively. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、食料品や医薬品の製造に活用される微生物濃度調整装置に関するものである。   The present invention relates to a microorganism concentration adjusting apparatus used for the production of foods and pharmaceuticals.

従来のこの種、微生物濃度調整装置の構成は、以下のような構成となっていた。   The configuration of this type of conventional microorganism concentration adjusting apparatus has the following configuration.

すなわち、微生物濃度調整器と、この微生物濃度調整器の上流側に、選択的に接続される培養液容器および洗浄液容器と、前記微生物濃度調整器の下流側に接続される培養液収納容器とを備え、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさが同じ複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成となっていた（例えば下記特許文献１）。   That is, a microorganism concentration adjuster, a culture solution container and a cleaning solution container selectively connected upstream of the microorganism concentration adjuster, and a culture solution storage container connected downstream of the microorganism concentration adjuster The microorganism concentration controller includes a plurality of microorganism capture electrode pairs having the same size of the gap between the opposed electrodes and the opposed electrodes, and voltage supply means connected to the plurality of microorganism capture electrode pairs, respectively. (For example, Patent Document 1 below).

国際公開第２００８／１４９７９７号International Publication No. 2008/149797

上記従来例における課題は、希望する微生物濃度の培養液を得ることが出来ないということであった。   The problem in the conventional example is that a culture solution having a desired microorganism concentration cannot be obtained.

すなわち、上記従来例の微生物濃度調整装置は、複数の微生物捕獲電極対で捕獲した微生物を培養液収納容器に濃縮回収するものであって、希望する微生物濃度の培養液を得ることができないものであった。   That is, the above-described conventional microorganism concentration adjusting apparatus concentrates and collects microorganisms captured by a plurality of microorganism capture electrode pairs in a culture solution storage container, and cannot obtain a culture solution having a desired microorganism concentration. there were.

そこで本発明は、希望する微生物濃度の培養液を得ることを目的とするものである。   Accordingly, an object of the present invention is to obtain a culture solution having a desired microorganism concentration.

そして、この目的を達成するために本発明は、微生物濃度調整器と、この微生物濃度調整器の上流側に、選択的に接続される培養液容器および洗浄液容器と、前記微生物濃度調整器の下流側に接続される培養液収納容器とを備え、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成とし、これにより所期の目的を達成するものである。   In order to achieve this object, the present invention provides a microorganism concentration adjuster, a culture solution container and a washing solution container selectively connected upstream of the microorganism concentration adjuster, and a downstream of the microorganism concentration adjuster. A microorganism storage electrode connected to the side, wherein the microorganism concentration adjuster includes a plurality of microorganism capture electrode pairs in which at least one of the size of the opposed electrode and the gap between the opposed electrodes is different, and the plurality of microorganisms The voltage supply means is connected to each of the capture electrode pairs, thereby achieving the intended purpose.

以上のように本発明は、微生物濃度調整器と、この微生物濃度調整器の上流側に、選択的に接続される培養液容器および洗浄液容器と、前記微生物濃度調整器の下流側に接続される培養液収納容器とを備え、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成としたものであるので、希望する微生物濃度の培養液を得ることができる。   As described above, the present invention is connected to the microorganism concentration adjuster, the culture solution container and the washing solution container selectively connected to the upstream side of the microorganism concentration adjuster, and the downstream side of the microorganism concentration adjuster. A plurality of microorganism capture electrode pairs, each having a gap between opposing electrodes and at least one of the opposing electrode sizes, and each of the plurality of microorganism capture electrode pairs. Since it has a configuration having a connected voltage supply means, a culture solution having a desired microorganism concentration can be obtained.

すなわち、本発明においては、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成としたものであるので、希望する微生物濃度に応じ、微生物を捕獲した複数の微生物捕獲電極対から捕獲微生物を開放し、それを培養液収納容器に供給することで、希望する微生物濃度の培養液を得ることができるのである。   That is, in the present invention, the microorganism concentration adjuster is connected to a plurality of microorganism capture electrode pairs in which at least one of the gap between the opposing electrodes and the size of the opposing electrodes is different, and the plurality of microorganism capture electrode pairs, respectively. Therefore, according to the desired concentration of microorganisms, the captured microorganisms are released from a plurality of microorganism capture electrode pairs that have captured microorganisms and supplied to the culture solution storage container. A culture solution having a desired microorganism concentration can be obtained.

本発明の一実施形態に係る微生物濃度調整装置の構成図The block diagram of the microorganisms concentration adjustment apparatus which concerns on one Embodiment of this invention. その制御ブロック図Control block diagram その微生物濃度調整器の構成図Configuration diagram of the microorganism concentration regulator その微生物濃度調整器の捕獲電極対を示す斜視図The perspective view which shows the capture electrode pair of the microorganisms concentration regulator その動作説明を行う構成図Configuration diagram explaining its operation その動作説明を行う構成図Configuration diagram explaining its operation その動作説明を行う構成図Configuration diagram explaining its operation

以下、本発明の一実施形態を添付図面を用いて説明する。   Hereinafter, an embodiment of the present invention will be described with reference to the accompanying drawings.

図１において、１は微生物濃度調整器で、その液体流入口２には、ポンプ３、切替弁４を介して培養液収納容器５が接続されている。また、切替弁４には、切替弁６を介して洗浄液収納容器７と試料液収納容器８が選択的に接続されている。   In FIG. 1, reference numeral 1 denotes a microorganism concentration adjuster, and a culture solution storage container 5 is connected to a liquid inlet 2 via a pump 3 and a switching valve 4. Further, a cleaning liquid storage container 7 and a sample liquid storage container 8 are selectively connected to the switching valve 4 via a switching valve 6.

さらに、微生物濃度調整器１の液体流出口９には、切替弁１０を介して培養液収納容器１１が接続されている。また、切替弁１０には、切替弁１２を介して、培養液収納容器５と廃棄槽１３が選択的に接続されている。   Furthermore, a culture solution storage container 11 is connected to the liquid outlet 9 of the microorganism concentration adjuster 1 via a switching valve 10. Further, the culture solution storage container 5 and the waste tank 13 are selectively connected to the switching valve 10 via the switching valve 12.

さて、本実施形態における特徴は、微生物濃度調整器１を図３に示す構成としたものである。すなわち、液体流入口２と液体流出口９の間には液体流路１４が設けられ、この液体流路１４には、その液体流入口２から液体流出口９に向けて、１２対の微生物捕獲電極対１５ａ、１５ｂ、１５ｃ、１５ｄ、１５ｅ、１５ｆ、１５ｇ、１５ｈ、１５ｉ、１５ｊ、１５ｋ、１５ｌが直線状に配置されている。   The feature of the present embodiment is that the microorganism concentration adjuster 1 is configured as shown in FIG. That is, a liquid channel 14 is provided between the liquid inlet 2 and the liquid outlet 9, and 12 pairs of microorganisms are captured in the liquid channel 14 from the liquid inlet 2 toward the liquid outlet 9. The electrode pairs 15a, 15b, 15c, 15d, 15e, 15f, 15g, 15h, 15i, 15j, 15k, 15l are linearly arranged.

これら１２対の微生物捕獲電極対１５ａ〜１５ｌは、それぞれ櫛歯状の電極を所定のギャップを介して対向させた構成となっている。   Each of these 12 pairs of microorganism capture electrodes 15a to 15l has a configuration in which comb-like electrodes are opposed to each other with a predetermined gap therebetween.

しかし、これらの微生物捕獲電極対１５ａ〜１５ｌは、以下に説明する微生物の捕獲個数に応じて、対向する電極間のギャップと、対向する電極の大きさ（長さ）が異ならせている。   However, in these microorganism capture electrode pairs 15a to 15l, the gap between the opposed electrodes and the size (length) of the opposed electrodes are varied according to the number of captured microorganisms described below.

具体的には、微生物捕獲電極対１５ａは微生物を１×１０Ｅ０６（１０の６乗）、１５ｂは微生物を１×１０Ｅ０６（１０の６乗）、１５ｃは微生物を３×１０Ｅ０６（１０の６乗）、１５ｄは微生物を５×１０Ｅ０６（１０の６乗）、１５ｅは微生物を１×１０Ｅ０７（１０の７乗）、１５ｆは微生物を１×１０Ｅ０７（１０の７乗）、１５ｇは微生物を３×１０Ｅ０７（１０の７乗）、１５ｈは微生物を５×１０Ｅ０７（１０の７乗）、１５ｉは微生物を１×１０Ｅ０８（１０の８乗）、１５ｊは微生物を１×１０Ｅ０８（１０の８乗）、１５ｋは微生物を３×１０Ｅ０８（１０の８乗）、１５ｌは微生物を５×１０Ｅ０８（１０の８乗）を捕獲する構成となっている。   Specifically, the microorganism capture electrode pair 15a is 1 × 10E06 (10 6), 15b is 1 × 10E06 (10 6), and 15c is 3 × 10E06 (10 6). 15d is 5 × 10E06 (10 6), 15e is 1 × 10E07 (10 7), 15f is 1 × 10E07 (10 7), 15g is 3 × 10E07 (10 to the seventh power), 15h for microorganisms 5 × 10E07 (10 to the seventh power), 15i for microorganisms 1 × 10E08 (10 to the eighth power), 15j for microorganisms 1 × 10E08 (10 to the eighth power), 15k Is configured to capture 3 × 10E08 (10 8) of microorganisms, and 15 l is configured to capture 5 × 10E08 (10 8) of microorganisms.

そして、このような微生物の捕獲をさせるために、これらの微生物捕獲電極対１５ａ〜１５ｌには、それぞれ図２、図３で示すように、ドライブ回路１６を介して、電圧供給手段１７から電圧が供給される構成となっている。   In order to capture such microorganisms, a voltage is supplied to these microorganism capture electrode pairs 15a to 15l from the voltage supply means 17 via the drive circuit 16 as shown in FIGS. It is a configuration to be supplied.

すなわち、電圧供給手段１７は、希望する微生物濃度を設定する入力部１８と、この入力部１８に接続された制御部１９と、この制御部１９に接続された表示部２０と、これらに電圧を供給する電源部２１とにより構成されている。   That is, the voltage supply means 17 includes an input unit 18 for setting a desired microorganism concentration, a control unit 19 connected to the input unit 18, a display unit 20 connected to the control unit 19, and a voltage applied thereto. It is comprised by the power supply part 21 to supply.

また、ドライブ回路１６は、制御部２２と、この制御部２２に接続された捕獲電圧供給部２３、および測定電圧供給部２４と、前記捕獲電圧供給部２３と微生物捕獲電極対１５ａ間に設けたスイッチング手段２５と、前記測定電圧供給部２４と微生物捕獲電極対１５ａ間に設けたスイッチング手段２６とにより構成されている。   The drive circuit 16 is provided between the control unit 22, the capture voltage supply unit 23 and the measurement voltage supply unit 24 connected to the control unit 22, and the capture voltage supply unit 23 and the microorganism capture electrode pair 15a. The switching means 25 and the switching means 26 provided between the measurement voltage supply unit 24 and the microorganism capturing electrode pair 15a are configured.

なお、ドライブ回路１６は、微生物捕獲電極対１５ａ〜１５ｌとも共通のものを用いている。ただし、入力部１８によって設定した微生物の捕獲個数に応じて、これらの微生物捕獲電極対１５ａ〜１５ｌに選択的に電源供給が行われる構成となっている。   Note that the drive circuit 16 is common to the microorganism capture electrode pairs 15a to 15l. However, according to the number of microorganisms captured by the input unit 18, power is selectively supplied to these microorganism capture electrode pairs 15a to 15l.

以上の構成において、図５は、微生物濃度調整器１の液体流路１４に培養液収納容器５から微生物を含む培養液を供給する状態を示している。   In the above configuration, FIG. 5 shows a state in which a culture solution containing microorganisms is supplied from the culture solution storage container 5 to the liquid channel 14 of the microorganism concentration controller 1.

この場合、まず、電圧供給手段１７の電源スイッチ２７（図２には、図示していないが電源部２１に接続されているものである）を入れ、図２に示す全ての微生物捕獲電極対１５ａ〜１５ｌにドライブ回路１６を介して電圧を供給する。   In this case, first, a power switch 27 (not shown in FIG. 2 but connected to the power supply unit 21) of the voltage supply means 17 is turned on, and all the microorganism capture electrode pairs 15a shown in FIG. A voltage is supplied to ˜15 l via the drive circuit 16.

また、ポンプ３および切替弁４、１０、１２に通電し、これにより、培養液収納容器５から微生物を含む培養液を、切替弁４、ポンプ３を介して微生物濃度調整器１の液体流路１４に供給し、また、余分な培養液は、切替弁１０、１２を介して、培養液収納容器５に戻すこととする。   In addition, the pump 3 and the switching valves 4, 10, 12 are energized, whereby the culture solution containing microorganisms is transferred from the culture solution storage container 5 via the switching valve 4 and the pump 3 to the liquid flow path of the microorganism concentration regulator 1. 14 and the excess culture solution is returned to the culture solution storage container 5 via the switching valves 10 and 12.

この結果、全ての微生物捕獲電極対１５ａ〜１５ｌに、上述した設定個数の微生物がそれぞれ捕獲されることとなる。   As a result, the set number of microorganisms described above are captured by all the microorganism capturing electrode pairs 15a to 15l.

この場合、ドライブ回路１６においては、捕獲電圧供給部２３からスイッチング手段２５を介して、微生物捕獲電極対１５ａ〜１５ｌに微生物捕獲のための３ＭＨｚの電圧が供給され、これにより、周知の誘電泳動により、微生物捕獲電極対１５ａ〜１５ｌに設定個数通りの微生物が捕獲される。   In this case, in the drive circuit 16, a voltage of 3 MHz for capturing microorganisms is supplied from the capture voltage supply unit 23 via the switching means 25 to the microorganism capture electrode pairs 15 a to 15 l, thereby performing known dielectrophoresis. The set number of microorganisms are captured by the microorganism capture electrode pairs 15a to 15l.

なお、それぞれの微生物捕獲電極対１５ａ〜１５ｌで設定個数通りの微生物が捕獲された否かを測定するために、捕獲電圧供給部２３を一時停止し、測定電圧供給部２４から８００ｋＨｚの測定電圧をスイッチング手段２６を介して、微生物捕獲電極対１５ａ〜１５ｌに測定電圧を供給し、測定を行う。   In addition, in order to measure whether or not a set number of microorganisms are captured by each of the microorganism capture electrode pairs 15a to 15l, the capture voltage supply unit 23 is temporarily stopped, and a measurement voltage of 800 kHz is applied from the measurement voltage supply unit 24. A measurement voltage is supplied to the microorganism capture electrode pairs 15a to 15l via the switching means 26 to perform measurement.

そして、この測定により、全ての微生物捕獲電極対１５ａ〜１５ｌにおいて、設定個数通りの微生物が捕獲されると、入力部１８により、培養液収納容器１１に収納させる微生物濃度を設定する。   Then, by this measurement, when the set number of microorganisms are captured in all the microorganism capture electrode pairs 15a to 15l, the input unit 18 sets the concentration of microorganisms to be stored in the culture solution storage container 11.

すると、図６に示すごとく、まず、切替弁４の通電を断ち、切替弁６に通電し、これにより、洗浄液収納容器７から洗浄液として純水を切替弁６、切替弁４、ポンプ３を介して微生物濃度調整器１の液体流路１４に供給する。そして、切替弁１２の通電を断つ。   Then, as shown in FIG. 6, first, the switching valve 4 is turned off and the switching valve 6 is turned on. As a result, pure water is supplied from the cleaning liquid storage container 7 as the cleaning liquid via the switching valve 6, the switching valve 4, and the pump 3. And supplied to the liquid flow path 14 of the microorganism concentration controller 1. Then, the switching valve 12 is turned off.

その結果、液体流路１４内において、微生物捕獲電極対１５ａ〜１５ｌで捕獲されていない微生物は切替弁１０、１２を介して廃棄槽１３に排出される。   As a result, in the liquid flow path 14, the microorganisms that are not captured by the microorganism capture electrode pairs 15 a to 15 l are discharged to the waste tank 13 through the switching valves 10 and 12.

また、液体流路１４内において、微生物捕獲電極対１５ａ〜１５ｌに捕獲されている微生物は、栄養素を全く含まない純水が周りを覆ったことで、この液体流路１４内において、増殖することはない。   In addition, the microorganisms captured by the microorganism capture electrode pairs 15a to 15l in the liquid channel 14 grow in the liquid channel 14 because the pure water not containing any nutrients covers the surroundings. There is no.

つまり、この状態においては、液体流路１４内において、微生物捕獲電極対１５ａ〜１５ｌで設定された通りの微生物が、存在している状態となっている。   That is, in this state, microorganisms as set by the microorganism capture electrode pairs 15a to 15l exist in the liquid flow path 14.

ここで、この部分について、さらに詳細に説明すると、通電された微生物捕獲電極対１５ａ〜１５ｌは、上述のごとく、個々に設定された通りの個数の微生物を捕獲しているが、入力部１８によって設定された範囲外の微生物捕獲電極対１５ａ〜１５ｌは、その通電が解かれ、微生物の捕獲も開放され、この開放された微生物は、液体流路１４内に供給される純水とともに廃棄槽１３に向けて排出される。   Here, this part will be described in more detail. The energized microorganism capturing electrode pairs 15a to 15l capture the number of microorganisms as set individually as described above. The microorganism capture electrode pairs 15a to 15l outside the set range are de-energized and the capture of microorganisms is also released. The opened microorganisms are disposed in the waste tank 13 together with pure water supplied into the liquid flow path 14. It is discharged toward

そこで、この状態において、次に図７のごとく、切替弁６の通電を断ち、これにより、試料液収納容器８からの試料を切替弁６、４、ポンプ３を介して微生物濃度調整器１の液体流路１４に試料液（例えば、純水または設定された試料液）を供給し、この時に微生物捕獲電極対１５ａ〜１５ｌへの通電を絶ち、切替弁１０の通電を絶つ。   Therefore, in this state, as shown in FIG. 7, next, the energization of the switching valve 6 is cut off, so that the sample from the sample solution storage container 8 is supplied to the microorganism concentration regulator 1 via the switching valves 6, 4 and the pump 3. A sample solution (for example, pure water or a set sample solution) is supplied to the liquid flow path 14, and at this time, the energization of the microorganism capture electrode pairs 15a to 15l is stopped, and the switching valve 10 is turned off.

すると、微生物捕獲電極対１５ａ〜１５ｌによって捕獲された設定個数通りの微生物が切替弁１０を介して、培養液収納容器１１に試料液とともに流入することとなる。   Then, a set number of microorganisms captured by the microorganism capture electrode pairs 15a to 15l flow into the culture solution storage container 11 through the switching valve 10 together with the sample solution.

この時、培養液収納容器１１内に流入した微生物の個数は、入力部１８によって設定された通りのものとなり、これが、培養液収納容器１１内に供給されるので、この培養液収納容器１１内における微生物濃度は、設定された通りのものとなる。   At this time, the number of microorganisms flowing into the culture solution storage container 11 is as set by the input unit 18 and is supplied into the culture solution storage container 11. The microbial concentration in is as set.

なお、微生物捕獲電極対１５ａ〜１５ｌの設定として、微生物捕獲電極対１５ａは微生物を１×１０Ｅ０６（１０の６乗）、１５ｂは微生物を１×１０Ｅ０６（１０の６乗）、１５ｃは微生物を３×１０Ｅ０６（１０の６乗）、１５ｄは微生物を５×１０Ｅ０６（１０の６乗）、１５ｅは微生物を１×１０Ｅ０７（１０の７乗）、１５ｆは微生物を１×１０Ｅ０７（１０の７乗）、１５ｇは微生物を３×１０Ｅ０７（１０の７乗）、１５ｈは微生物を５×１０Ｅ０７（１０の７乗）、１５ｉは微生物を１×１０Ｅ０８（１０の８乗）、１５ｊは微生物を１×１０Ｅ０８（１０の８乗）、１５ｋは微生物を３×１０Ｅ０８（１０の８乗）、１５ｌは微生物を５×１０Ｅ０８（１０の８乗）とした理由は、これらの通電、あるいは通電停止を組み合わせれば、複数の設定値が簡単に得られるからである。   The microorganism capture electrode pairs 15a to 15l are set as follows: the microorganism capture electrode pair 15a is 1 × 10E06 (10 6), 15b is 1 × 10E06 (10 6), and 15c is 3 microorganisms. × 10E06 (10 6), 15d is 5 × 10E06 (10 6), 15e is 1 × 10E07 (10 7), 15f is 1 × 10E07 (10 7) 15g is 3 × 10E07 (10 to the 7th power), 15h is 5 × 10E07 (10 to the 7th power), 15i is 1 × 10E08 (10 to the 8th power), 15j is 1 × 10E08. (10 to the 8th power), 15k for microorganisms 3 × 10E08 (10 to the 8th power), and 15l for microorganisms 5 × 10E08 (10 to the 8th power). This is because a plurality of set values can be easily obtained.

例えば、捕獲する微生物の個数を９×１０Ｅ０６（１０の６乗）個とする場合には、微生物捕獲電極対１５ｂ〜１５ｄの３つに通電し、他は非通電状態とすればよい。   For example, when the number of microorganisms to be captured is 9 × 10E06 (10 6), three of the microorganism capture electrode pairs 15b to 15d may be energized, and the other may be in a non-energized state.

また、捕獲する微生物の個数を９×１０Ｅ０７（１０の７乗）個とする場合には、微生物捕獲電極対１５ｆ〜１５ｈの３つに通電し、他は非通電状態とすればよい。   Further, when the number of microorganisms to be captured is 9 × 10E07 (10 to the seventh power), the three microorganism capture electrode pairs 15f to 15h may be energized and the others may be in a non-energized state.

このように、本実施形態においては、複数設けた微生物捕獲電極対１５ａ〜１５ｌのギャップ間の距離や大きさを異ならせると共に、選択的な通電を行うことで、微生物濃度を希望する濃度に簡単に調整することができるものとなる。   As described above, in the present embodiment, the distance and size between the gaps of the plurality of microorganism capture electrode pairs 15a to 15l that are provided are made different, and selective energization is performed to easily reduce the microorganism concentration to a desired concentration. Can be adjusted.

なお、上記実施形態においては、希望する微生物の捕獲個数に関わらず、図５、図６の状態では、全ての微生物捕獲電極対１５ａ〜１５ｌに通電し、これら全ての微生物捕獲電極対１５ａ〜１５ｌで微生物を捕獲し、その後、選択的に微生物捕獲電極対１５ａ〜１５ｌの通電を絶つことで、図６の純水に流すものとしたが、図５の状態において、全ての微生物捕獲電極対１５ａ〜１５ｌの中から選んだ捕獲させる微生物捕獲電極対１５ａ〜１５ｌの数個だけに通電させる構成としてもよい。   In the above embodiment, regardless of the desired number of microorganisms to be captured, in the state of FIGS. 5 and 6, all the microorganism capture electrode pairs 15a to 15l are energized and all these microorganism capture electrode pairs 15a to 15l are energized. Then, the microorganisms are captured in the pure water shown in FIG. 6 by selectively deenergizing the microorganism-capturing electrode pairs 15a to 15l. However, in the state shown in FIG. A configuration may be adopted in which only some of the microorganism capture electrode pairs 15a to 15l to be captured selected from ˜15l are energized.

以上のように本発明は、微生物濃度調整器と、この微生物濃度調整器の上流側に、選択的に接続される培養液容器および洗浄液容器と、前記微生物濃度調整器の下流側に接続される培養液収納容器とを備え、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成としたものであるので、希望する微生物濃度の培養液を得ることができる。   As described above, the present invention is connected to the microorganism concentration adjuster, the culture solution container and the washing solution container selectively connected to the upstream side of the microorganism concentration adjuster, and the downstream side of the microorganism concentration adjuster. A plurality of microorganism capture electrode pairs, each having a gap between opposing electrodes and at least one of the opposing electrode sizes, and each of the plurality of microorganism capture electrode pairs. Since it has a configuration having a connected voltage supply means, a culture solution having a desired microorganism concentration can be obtained.

すなわち、本発明においては、前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する構成としたものであるので、希望する微生物濃度に応じ、微生物を捕獲した複数の微生物捕獲電極対から捕獲微生物を開放し、それを培養液収納容器に供給することで、希望する微生物濃度の培養液を得ることができるのである。   That is, in the present invention, the microorganism concentration adjuster is connected to a plurality of microorganism capture electrode pairs in which at least one of the gap between the opposing electrodes and the size of the opposing electrodes is different, and the plurality of microorganism capture electrode pairs, respectively. Therefore, according to the desired concentration of microorganisms, the captured microorganisms are released from a plurality of microorganism capture electrode pairs that have captured microorganisms and supplied to the culture solution storage container. A culture solution having a desired microorganism concentration can be obtained.

したがって、微生物濃度調整装置として、広く活用が期待されるものである。   Therefore, it is expected to be widely used as a microorganism concentration adjusting device.

１ 微生物濃度調整器
２ 液体流入口
３ ポンプ
４ 切替弁
５ 培養液収納容器
６ 切替弁
７ 洗浄液収納容器
８ 試料液収納容器
９ 液体流出口
１０ 切替弁
１１ 培養液収納容器
１２ 切替弁
１３ 廃棄槽
１４ 液体流路
１５ａ，１５ｂ，１５ｃ，１５ｄ，１５ｅ，１５ｆ，１５ｇ，１５ｈ，１５ｉ，１５ｊ，１５ｋ，１５ｌ 微生物捕獲電極対
１６ ドライブ回路
１７ 電圧供給手段
１８ 入力部
１９ 制御部
２０ 表示部
２１ 電源部
２２ 制御部
２３ 捕獲電圧供給部
２４ 測定電圧供給部
２５ スイッチング手段
２６ スイッチング手段
２７ 電源スイッチ DESCRIPTION OF SYMBOLS 1 Microbial concentration regulator 2 Liquid inlet 3 Pump 4 Switching valve 5 Culture liquid storage container 6 Switching valve 7 Washing liquid storage container 8 Sample liquid storage container 9 Liquid outlet 10 Switching valve 11 Culture liquid storage container 12 Switching valve 13 Disposal tank 14 Liquid flow path 15a, 15b, 15c, 15d, 15e, 15f, 15g, 15h, 15i, 15j, 15k, 15l Microorganism capture electrode pair 16 Drive circuit 17 Voltage supply means 18 Input unit 19 Control unit 20 Display unit 21 Power supply unit 22 Control unit 23 Capture voltage supply unit 24 Measurement voltage supply unit 25 Switching means 26 Switching means 27 Power switch

Claims (5)

微生物濃度調整器と、この微生物濃度調整器の上流側に、選択的に接続される培養液容器および洗浄液容器と、前記微生物濃度調整器の下流側に接続される培養液収納容器とを備え、
前記微生物濃度調整器は、対向する電極間のギャップと対向する電極の大きさの少なくとも一方が異なる複数の微生物捕獲電極対と、これら複数の微生物捕獲電極対にそれぞれ接続した電圧供給手段とを有する微生物濃度調整装置。 A microorganism concentration adjuster, a culture solution container and a washing solution container selectively connected upstream of the microorganism concentration adjuster, and a culture solution storage container connected downstream of the microorganism concentration adjuster;
The microorganism concentration adjuster includes a plurality of microorganism capture electrode pairs in which at least one of the sizes of the opposed electrodes and the gap between the opposed electrodes is different, and voltage supply means respectively connected to the plurality of microorganism capture electrode pairs. Microbial concentration adjustment device. 洗浄液容器は、純水を収納する構成とした請求項１に記載の微生物濃度調整装置。   The microorganism concentration adjusting apparatus according to claim 1, wherein the cleaning liquid container is configured to store pure water. 微生物濃度調整器と培養液収納容器との間に培養液容器への帰還路を設けた請求項１または２に記載の微生物濃度調整装置。   The microorganism concentration adjusting apparatus according to claim 1 or 2, wherein a return path to the culture solution container is provided between the microorganism concentration adjuster and the culture solution storage container. 微生物濃度調整器と培養液収納容器との間に廃棄槽を接続した請求項１から３のいずれか一つに記載の微生物濃度調整装置。   The microorganism concentration adjusting apparatus according to any one of claims 1 to 3, wherein a waste tank is connected between the microorganism concentration adjusting device and the culture solution storage container. 電圧供給手段は、希望する微生物濃度を設定する入力部と、この入力部に接続された制御部と、この制御部に接続された表示部とを有し、前記制御部に複数の微生物捕獲電極対を接続した請求項１から４のいずれか一つに記載の微生物濃度調整装置。   The voltage supply means includes an input unit for setting a desired microorganism concentration, a control unit connected to the input unit, and a display unit connected to the control unit, and the control unit includes a plurality of microorganism capture electrodes. The microorganism concentration adjusting apparatus according to any one of claims 1 to 4, wherein a pair is connected.
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