JP2010516238A5 - - Google Patents
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- JP2010516238A5 JP2010516238A5 JP2009545964A JP2009545964A JP2010516238A5 JP 2010516238 A5 JP2010516238 A5 JP 2010516238A5 JP 2009545964 A JP2009545964 A JP 2009545964A JP 2009545964 A JP2009545964 A JP 2009545964A JP 2010516238 A5 JP2010516238 A5 JP 2010516238A5
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- cells
- glycan
- cell
- cell population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 210000004027 cell Anatomy 0.000 claims 29
- 102000004190 Enzymes Human genes 0.000 claims 28
- 108090000790 Enzymes Proteins 0.000 claims 28
- 150000004676 glycans Chemical class 0.000 claims 16
- 238000000034 method Methods 0.000 claims 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 12
- LQEBEXMHBLQMDB-UHFFFAOYSA-N [[5-(2-amino-6-oxo-3h-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] (3,4,5-trihydroxy-6-methyloxan-2-yl) hydrogen phosphate Chemical compound OC1C(O)C(O)C(C)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C3=C(C(N=C(N)N3)=O)N=C2)O1 LQEBEXMHBLQMDB-UHFFFAOYSA-N 0.000 claims 8
- 230000009450 sialylation Effects 0.000 claims 7
- 102000006471 Fucosyltransferases Human genes 0.000 claims 6
- 108010019236 Fucosyltransferases Proteins 0.000 claims 6
- 102000003838 Sialyltransferases Human genes 0.000 claims 6
- 108090000141 Sialyltransferases Proteins 0.000 claims 6
- 229960002685 biotin Drugs 0.000 claims 6
- 235000020958 biotin Nutrition 0.000 claims 6
- 239000011616 biotin Substances 0.000 claims 6
- 102000005348 Neuraminidase Human genes 0.000 claims 5
- 108010006232 Neuraminidase Proteins 0.000 claims 5
- 102000004357 Transferases Human genes 0.000 claims 5
- 108090000992 Transferases Proteins 0.000 claims 5
- 238000000338 in vitro Methods 0.000 claims 5
- 239000003112 inhibitor Substances 0.000 claims 5
- 210000004700 fetal blood Anatomy 0.000 claims 4
- 230000033581 fucosylation Effects 0.000 claims 4
- 239000011159 matrix material Substances 0.000 claims 4
- TXCIAUNLDRJGJZ-UHFFFAOYSA-N CMP-N-acetyl neuraminic acid Natural products O1C(C(O)C(O)CO)C(NC(=O)C)C(O)CC1(C(O)=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(N=C(N)C=C2)=O)O1 TXCIAUNLDRJGJZ-UHFFFAOYSA-N 0.000 claims 3
- TXCIAUNLDRJGJZ-BILDWYJOSA-N CMP-N-acetyl-beta-neuraminic acid Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@]1(C(O)=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)C=C2)=O)O1 TXCIAUNLDRJGJZ-BILDWYJOSA-N 0.000 claims 3
- FDJKUWYYUZCUJX-KVNVFURPSA-N N-glycolylneuraminic acid Chemical compound OC[C@H](O)[C@H](O)[C@@H]1O[C@](O)(C(O)=O)C[C@H](O)[C@H]1NC(=O)CO FDJKUWYYUZCUJX-KVNVFURPSA-N 0.000 claims 3
- 125000000217 alkyl group Chemical group 0.000 claims 3
- 150000001412 amines Chemical class 0.000 claims 3
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 125000006850 spacer group Chemical group 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- 125000003396 thiol group Chemical class [H]S* 0.000 claims 3
- 102100031974 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 Human genes 0.000 claims 2
- 101710083574 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 Proteins 0.000 claims 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims 2
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 claims 2
- 102000051366 Glycosyltransferases Human genes 0.000 claims 2
- 108700023372 Glycosyltransferases Proteins 0.000 claims 2
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 claims 2
- 210000001671 embryonic stem cell Anatomy 0.000 claims 2
- -1 his-tag Substances 0.000 claims 2
- 210000005260 human cell Anatomy 0.000 claims 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims 2
- 239000008279 sol Substances 0.000 claims 2
- 239000007790 solid phase Substances 0.000 claims 2
- KFEUJDWYNGMDBV-UHFFFAOYSA-N (N-Acetyl)-glucosamin-4-beta-galaktosid Natural products OC1C(NC(=O)C)C(O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 KFEUJDWYNGMDBV-UHFFFAOYSA-N 0.000 claims 1
- 102100029945 Beta-galactoside alpha-2,6-sialyltransferase 1 Human genes 0.000 claims 1
- 101710136191 Beta-galactoside alpha-2,6-sialyltransferase 1 Proteins 0.000 claims 1
- 102100029962 CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase Human genes 0.000 claims 1
- 101710136075 CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase Proteins 0.000 claims 1
- 102100027098 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 Human genes 0.000 claims 1
- 101710083568 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 Proteins 0.000 claims 1
- 102100031973 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 Human genes 0.000 claims 1
- 101710083563 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 Proteins 0.000 claims 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 claims 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 claims 1
- KFEUJDWYNGMDBV-LODBTCKLSA-N N-acetyllactosamine Chemical group O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KFEUJDWYNGMDBV-LODBTCKLSA-N 0.000 claims 1
- HESSGHHCXGBPAJ-UHFFFAOYSA-N N-acetyllactosamine Natural products CC(=O)NC(C=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O HESSGHHCXGBPAJ-UHFFFAOYSA-N 0.000 claims 1
- SQVRNKJHWKZAKO-LUWBGTNYSA-N N-acetylneuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)CC(O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-LUWBGTNYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000002860 competitive effect Effects 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 229930182470 glycoside Natural products 0.000 claims 1
- 230000013595 glycosylation Effects 0.000 claims 1
- 238000006206 glycosylation reaction Methods 0.000 claims 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 239000003446 ligand Substances 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 229920001542 oligosaccharide Polymers 0.000 claims 1
- 150000002482 oligosaccharides Chemical class 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 claims 1
- 125000005629 sialic acid group Chemical group 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000006177 thiolation reaction Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20075030A FI20075030A0 (fi) | 2007-01-18 | 2007-01-18 | Menetelmä solujen modifioimiseksi |
| PCT/FI2008/050015 WO2008087256A1 (en) | 2007-01-18 | 2008-01-18 | Method for modifying cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014096320A Division JP2014168476A (ja) | 2007-01-18 | 2014-05-07 | 細胞の修飾方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010516238A JP2010516238A (ja) | 2010-05-20 |
| JP2010516238A5 true JP2010516238A5 (https=) | 2011-03-10 |
Family
ID=37745722
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009545964A Pending JP2010516238A (ja) | 2007-01-18 | 2008-01-18 | 細胞の修飾方法 |
| JP2014096320A Pending JP2014168476A (ja) | 2007-01-18 | 2014-05-07 | 細胞の修飾方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014096320A Pending JP2014168476A (ja) | 2007-01-18 | 2014-05-07 | 細胞の修飾方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20100047892A1 (https=) |
| EP (1) | EP2118273A4 (https=) |
| JP (2) | JP2010516238A (https=) |
| CA (1) | CA2712425A1 (https=) |
| FI (1) | FI20075030A0 (https=) |
| WO (1) | WO2008087256A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2166085A1 (en) * | 2008-07-16 | 2010-03-24 | Suomen Punainen Risti Veripalvelu | Divalent modified cells |
| WO2010057110A1 (en) * | 2008-11-14 | 2010-05-20 | University Of Louisville Research Foundation, Inc. | Methods and compositions for long term hematopoietic repopulation |
| FI20105348A0 (fi) | 2010-04-06 | 2010-04-06 | Suomen Punainen Risti Veripalv | Proteolyyttisen entsyymin käyttö |
| US9788539B2 (en) | 2011-05-17 | 2017-10-17 | Velico Medical, Inc. | Platelet protection solution having beta-galactosidase and sialidase inhibitors |
| JP5856029B2 (ja) | 2012-08-31 | 2016-02-09 | 阿部 博幸 | 間葉系幹細胞を未分化増殖させる方法、および間葉系幹細胞を濃縮する方法 |
| EP2903430A1 (en) | 2012-10-05 | 2015-08-12 | Velico Medical, Inc. | Platelet additive solution having a beta-galactosidase inhibitor |
| US11312940B2 (en) | 2015-08-31 | 2022-04-26 | University Of Louisville Research Foundation, Inc. | Progenitor cells and methods for preparing and using the same |
| US11684638B2 (en) * | 2016-02-16 | 2023-06-27 | Regents Of The University Of Minnesota | Targeted delivery system, system components, and methods |
| WO2017152073A1 (en) | 2016-03-04 | 2017-09-08 | University Of Louisville Research Foundation, Inc. | Methods and compositions for ex vivo expansion of very small embryonic-like stem cells (vsels) |
| CN117007655B (zh) * | 2023-05-11 | 2025-09-05 | 中国干细胞集团上海生物科技有限公司 | 用于电化学检测造血干细胞的组合物及其方法 |
| CN117405745B (zh) * | 2023-10-27 | 2025-09-05 | 中国干细胞集团上海生物科技有限公司 | 基于邻近点击标记的造血干细胞传感方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6331658B1 (en) * | 1993-04-20 | 2001-12-18 | Integris Baptist Medical Center, Inc. | Genetically engineered mammals for use as organ donors |
| US5965457A (en) | 1995-06-06 | 1999-10-12 | Magnani; John L. | Methods of screening for a candidate compound able to bind to CD34+ cells |
| JP2001507215A (ja) * | 1997-01-16 | 2001-06-05 | サイテル コーポレイション | 組換え糖タンパク質のインビトロでの実用的なシアリル化 |
| US6232450B1 (en) * | 1998-11-12 | 2001-05-15 | The Scripps Research Institute | Inhibition of human fucosyltransferases with N-linked Lewis-x and LacNAc analogs |
| AU2001281463A1 (en) * | 2000-03-16 | 2001-09-24 | The Regents Of The University Of California | Chemoselective ligation |
| JP2004528001A (ja) * | 2000-05-12 | 2004-09-16 | ネオーズ テクノロジーズ， インコーポレイテッド | インビトロにおけるフコシル化組換えグリコペプチド |
| US7166278B2 (en) | 2001-04-30 | 2007-01-23 | Rbc Biotechnology, Inc. | Modified organs and cells for xenotransplantation |
| FI20011671A7 (fi) | 2001-08-20 | 2003-02-21 | Carbion Oy | Tuumorispesifiset oligosakkaridisekvenssit ja niiden käyttö |
| US7265084B2 (en) | 2001-10-10 | 2007-09-04 | Neose Technologies, Inc. | Glycopegylation methods and proteins/peptides produced by the methods |
| US7282222B2 (en) | 2002-03-15 | 2007-10-16 | The United States Of America As Represented By The Department Of Veterans Affairs | Methods and compositions for directing cells to target organs |
| US20060014717A1 (en) * | 2002-06-28 | 2006-01-19 | Glykos Finland Oy | Therapeutic compositions for use in prophylaxis or treatment of diarrheas |
| US20070141646A1 (en) | 2003-02-17 | 2007-06-21 | Henry Stephen M | Preparation of red blood cells with a modified level of blood group antigen expression and their use in the quality control of blood typing reagents |
| US7332334B2 (en) * | 2003-04-18 | 2008-02-19 | Oklahoma Medical Research Foundation | Hematopoietic stem cells treated by in vitro fucosylation and methods of use |
| BRPI0412671A (pt) | 2003-08-08 | 2006-10-03 | Fresenius Kabi De Gmbh | conjugados de um polìmero e uma proteìna ligados por um grupo de ligação de oxima |
| WO2005051429A2 (en) | 2003-11-19 | 2005-06-09 | Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Targeted conjugates with a modified saccharide linker |
| ES2390885T3 (es) | 2004-03-11 | 2012-11-19 | Fresenius Kabi Deutschland Gmbh | Conjugados de hidroxialquilalmidón y una proteína |
| EP1893756B1 (en) * | 2005-06-08 | 2015-08-12 | The Regents of The University of California | Elimination of n-glycolylneuraminic acid from mammalian products for human use |
| FI20055398A0 (fi) * | 2005-07-08 | 2005-07-08 | Suomen Punainen Risti Veripalv | Menetelmä solupopulaatioiden evaluoimiseksi |
| WO2007006870A2 (en) * | 2005-07-11 | 2007-01-18 | Suomen Punainen Risti, Veripalvelu | Novel carbohydrate profile compositions from human cells and methods for analysis and modification thereof |
| WO2007054622A1 (en) * | 2005-11-08 | 2007-05-18 | Suomen Punainen Risti, Veripalvelu | Novel carbohydrate profile compositions from human cells and methods for analysis and modification thereof |
| EP3336174A1 (en) * | 2006-06-02 | 2018-06-20 | Robert Sackstein | Compositions and methods for modifying cell surface glycans |
-
2007
- 2007-01-18 FI FI20075030A patent/FI20075030A0/fi unknown
-
2008
- 2008-01-18 WO PCT/FI2008/050015 patent/WO2008087256A1/en not_active Ceased
- 2008-01-18 EP EP08701715A patent/EP2118273A4/en not_active Withdrawn
- 2008-01-18 US US12/522,853 patent/US20100047892A1/en not_active Abandoned
- 2008-01-18 CA CA2712425A patent/CA2712425A1/en not_active Abandoned
- 2008-01-18 JP JP2009545964A patent/JP2010516238A/ja active Pending
-
2014
- 2014-05-07 JP JP2014096320A patent/JP2014168476A/ja active Pending
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