JP2010286510A5

JP2010286510A5 -

Info

Publication number: JP2010286510A5
Application number: JP2009137842A
Authority: JP
Filing date: 2009-06-09
Publication date: 2012-07-12
Anticipated expiration: 2029-06-09

Description

このような目的を達成するため、本発明に係る観察装置は、収容部に収容された液体及び観察対象物に光源からの照明光を照射する照明光学系と、前記観察対象物及び前記収容部を介して前記照明光学系と対向する位置に配置された対物レンズを含み、前記観察対象物の像を結像する結像光学系とを備え、次式ｒ／（α−β−δ）＜ｔ＜ｒ／β及びβ＝ｓ／ｔを満たすことを特徴とする。但し、ｒは前記収容部の内面に内接する円の半径、αは前記対物レンズの開口数、δは前記照明光が前記液体を通過したときの最大屈折角、ｔは前記収容部の底面から実効的な前記光源までの距離、ｓは実効的な前記光源の半径である。 In order to achieve such an object, an observation apparatus according to the present invention includes an illumination optical system that irradiates illumination light from a light source onto a liquid and an observation object accommodated in an accommodation unit, and the observation object and the accommodation unit. And an imaging optical system that forms an image of the observation object, including an objective lens arranged at a position facing the illumination optical system via the following formula: r / (α−β−δ) < It is characterized by satisfying t <r / β and β = s / t. Where r is the radius of a circle inscribed in the inner surface of the housing portion, α is the numerical aperture of the objective lens, δ is the maximum refraction angle when the illumination light passes through the liquid, and t is from the bottom surface of the housing portion. The effective distance to the light source , s, is the effective radius of the light source .

Claims

An illumination optical system that irradiates illumination light from a light source onto the liquid and the observation object contained in the accommodation unit;
An imaging optical system that forms an image of the observation object, including an objective lens disposed at a position facing the illumination optical system via the observation object and the storage unit;
The following equation: r / (α−β−δ) <t <r / β
β = s / t
An observation apparatus characterized by satisfying
Where r is the radius of a circle inscribed in the inner surface of the housing portion, α is the numerical aperture of the objective lens, δ is the maximum refraction angle when the illumination light passes through the liquid, and t is from the bottom surface of the housing portion. The effective distance to the light source , s, is the effective radius of the light source .

The illumination optical system includes a lens system for passing illumination light emitted from the light source,
The observation apparatus according to claim 1, wherein a conjugate plane conjugate with the light source via the lens system is provided on the opposite side of the objective lens via the housing portion.

The illumination optical system includes a field stop that adjusts an illumination range of illumination light emitted from the light source, and an aperture stop that forms a radius of the effective light source through the lens system. The observation apparatus according to claim 2.

The illumination optical system includes a lens system for passing illumination light emitted from the light source,
The observation apparatus according to claim 1, wherein a conjugate plane conjugate with the light source via the lens system is provided on the objective lens side via the housing portion.

The observation apparatus according to claim 4, wherein the illumination optical system includes an aperture stop that forms a radius of the effective light source via the lens system.

The observation apparatus according to claim 1, wherein δ≈0.

The observation apparatus according to claim 1 , wherein a radius r of the housing portion is 5 mm or less.

The observation apparatus according to claim 1, further comprising an imaging unit having an imaging surface provided at an imaging position by the imaging optical system.