JP2010253372A - Gas-liquid stirrer - Google Patents

Gas-liquid stirrer Download PDF

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JP2010253372A
JP2010253372A JP2009105647A JP2009105647A JP2010253372A JP 2010253372 A JP2010253372 A JP 2010253372A JP 2009105647 A JP2009105647 A JP 2009105647A JP 2009105647 A JP2009105647 A JP 2009105647A JP 2010253372 A JP2010253372 A JP 2010253372A
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stirring
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drive shaft
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Makoto Kitano
誠 北野
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IHI Corp
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Abstract

<P>PROBLEM TO BE SOLVED: To increase the dissolving amount of gas in a liquid by making the stagnation time of the gas bubbles in the liquid longer than before. <P>SOLUTION: The gas-liquid stirrer includes a stirring tank 1 storing the liquid, a gas diffusion part 7 ejecting gas bubbles into the stirring tank 1, a drive shaft 2 suspended into the stirring tank 1 and a plurality of the stirring blades 3A and 3B provided to the drive shaft 2 in a multistage fashion, and the stirring blades 3A and 3B of the respective stages apply forces in mutually different directions to the liquid. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、気液攪拌装置に関する。   The present invention relates to a gas-liquid stirring apparatus.

下記特許文献１には、培地が封入された培養槽の中央に上下２段の攪拌翼を備えると共に酸素の気泡を下部から攪拌槽内に噴出し、攪拌翼を回転させることによって酸素を培地中に溶け込ませる培養槽の運転制御装置が開示されている。
なお、下記特許文献２には、特許文献１のように酸素（気体）を培地（液体）中に溶け込ませるものではないが、液体を貯留する攪拌槽の内壁に垂直方向に延在する複数の邪魔板を備えると共に攪拌槽の中央に攪拌翼を備え、この攪拌翼を回転させることにより液体を攪拌させる攪拌装置が開示されている。 In the following Patent Document 1, an upper and lower two-stage stirring blade is provided at the center of a culture tank in which a culture medium is sealed, oxygen bubbles are blown into the stirring tank from below, and the stirring blade is rotated to allow oxygen to enter the culture medium. An operation control device for a culture tank to be dissolved in a cell is disclosed.
In Patent Document 2 below, oxygen (gas) is not dissolved in the culture medium (liquid) as in Patent Document 1, but a plurality of pieces extending in the vertical direction on the inner wall of the stirring tank storing the liquid are disclosed. There is disclosed a stirring device that includes a baffle plate and a stirring blade in the center of the stirring tank, and rotates the stirring blade to stir the liquid.

特開２００７−２０２５００号公報JP 2007-202500 A 特開平０９−０２９０８４号公報JP 09-029084 A

ところで、気体が液体に溶け込む量は、気泡の液体中における滞留時間が長くなる程多くなることが知られている。特許文献１の技術の場合、攪拌翼の回転による培地の下降流によって気泡は一端降下してから上昇する動きをとるが、気泡が培養槽の底に接触する程に攪拌翼の回転数を上げると、これ以上回転数を上げても滞留時間を上げることができない。すなわち、気泡の最大下降量は気泡の噴出し位置と培養槽の底との差によって物理的に規定されるので、気泡を下降させることによる滞留時間の拡大には限界がある。   By the way, it is known that the amount of gas dissolved in the liquid increases as the residence time of the bubbles in the liquid increases. In the case of the technique of Patent Document 1, the bubbles move upward after being lowered by the downward flow of the medium due to the rotation of the stirring blades, but the number of rotations of the stirring blades is increased so that the bubbles contact the bottom of the culture tank. Even if the number of rotations is further increased, the residence time cannot be increased. That is, since the maximum amount of bubbles to be lowered is physically defined by the difference between the bubble ejection position and the bottom of the culture tank, there is a limit to the extension of the residence time by lowering the bubbles.

本発明は、上述した事情に鑑みてなされたものであり、液体内における気泡の滞留時間を従来よりも長くすることにより、気体の液体への溶け込み量を増大させることを目的とするものである。   The present invention has been made in view of the above-described circumstances, and an object thereof is to increase the amount of gas dissolved in a liquid by making the residence time of bubbles in the liquid longer than before. .

上記目的を達成するために、本発明では、第１の解決手段として、液体を貯留する攪拌槽と、攪拌槽内に気泡を噴出する散気部と、攪拌槽内に垂下された駆動軸と、該駆動軸に多段に設けられた複数の攪拌翼と、を備え、各段の攪拌翼は、互いに異なる方向の力を液体に与える、という手段を採用する。   In order to achieve the above object, in the present invention, as a first solving means, a stirring tank for storing a liquid, an air diffuser for jetting bubbles into the stirring tank, and a drive shaft suspended in the stirring tank, And a plurality of stirring blades provided in multiple stages on the drive shaft, and the stirring blades at each stage employ a means that applies forces in different directions to the liquid.

第２の解決手段として、上記第１の解決手段において、攪拌翼は２段に設けられ、下段の攪拌翼は液体に下向きの力を与え、上段の攪拌翼は液体に上向きの力を与える、という手段を採用する。   As the second solution, in the first solution, the stirring blades are provided in two stages, the lower stirring blade gives a downward force to the liquid, and the upper stirring blade gives an upward force to the liquid. Adopt the means.

第３の解決手段として、上記第１または第２の解決手段において、各段の攪拌翼は、各方向に押し出された液体が上下方向における各段の攪拌翼の中間位置で衝突するように、所定の位相差θを持つ姿勢で駆動軸に取り付けられている、という手段を採用する。   As a third solution, in the first or second solution, the stirrer blades of each stage collide at a middle position of the stirrer blades of each stage in the vertical direction with the liquid pushed out in each direction. A means of being attached to the drive shaft in a posture having a predetermined phase difference θ is adopted.

第４の解決手段として、上記第１〜第３いずれかの解決手段において、散気部は、攪拌槽の下部に設けられる、という手段を採用する。   As a fourth solving means, in the first to third solving means, a means is adopted in which the air diffuser is provided in the lower part of the stirring tank.

第５の解決手段として、上記第１〜第４いずれかの解決手段において、攪拌槽の内壁において攪拌翼の間に位置する部位に設けられ、気泡の上昇を邪魔する邪魔板をさらに備える、という手段を採用する。   As a fifth solving means, in any one of the first to fourth solving means, it is further provided with a baffle plate which is provided at a position located between the stirring blades on the inner wall of the stirring tank and obstructs the rise of bubbles. Adopt means.

本発明によれば、各段の攪拌翼は、互いに異なる方向の力を液体に与えるので、上下方向に２つの液体の対流が発生する。そして、これら２つの対流によって気泡が運ばれるので、液体中における気泡の滞留時間を従来よりも長くすることが可能であり、この結果として気体の液体への溶け込み量を従来よりも増大させることができる。   According to the present invention, the stirrer blades at each stage apply forces in different directions to the liquid, so that convection of two liquids occurs in the vertical direction. Since the bubbles are carried by these two convections, the residence time of the bubbles in the liquid can be made longer than before, and as a result, the amount of gas dissolved in the liquid can be increased more than before. it can.

本発明の一実施形態に係る細胞培養装置Ａの縦断面図である。It is a longitudinal cross-sectional view of the cell culture apparatus A which concerns on one Embodiment of this invention. 上記縦断面図のＸ−Ｘ線矢視図である。It is a XX arrow directional view of the above-mentioned longitudinal section.

以下、図面を参照して、本発明の一実施形態について説明する。
図１及び図２に示すように、本実施形態に係る細胞培養装置Ａは、培養槽１、駆動軸２、２つの攪拌翼３Ａ、３Ｂ、下部軸受４Ａ、上部軸受４Ｂ、連結器５、駆動モータ６、散気部７、４つの邪魔板８Ａ〜８Ｄ及び伝熱ジャケット９から構成されている。詳細は後述するが、この細胞培養装置Ａは、培養液Ｌを攪拌することにより酸素を培養液Ｌ中に溶け込ませて細胞を培養するものであり、気液攪拌装置の一種と考えることができる。 Hereinafter, an embodiment of the present invention will be described with reference to the drawings.
As shown in FIGS. 1 and 2, the cell culture apparatus A according to the present embodiment includes a culture tank 1, a drive shaft 2, two stirring blades 3A and 3B, a lower bearing 4A, an upper bearing 4B, a coupler 5, and a drive. The motor 6, the air diffuser 7, the four baffle plates 8 </ b> A to 8 </ b> D, and the heat transfer jacket 9 are configured. Although details will be described later, this cell culture device A is a device for culturing cells by dissolving the oxygen in the culture solution L by stirring the culture solution L, and can be considered as a kind of gas-liquid stirring device. .

培養槽１は、有底かつ円筒状の容器であり、図示するように中心軸線が鉛直方向となるように固定されている。このような培養槽１の内部には、所定量の培養液Ｌが貯留されている。この培養液Ｌは、培養対象となる細胞（より正確には粒子状の複数の細胞片）を含むと共に、当該細胞の栄養となる各種の栄養素を含む液体である。駆動軸２は、培養槽１の中心軸線に沿って上下方向に延在する棒状部材であり、途中部位の２箇所に攪拌翼３Ａ、３Ｂが固定されている。また、この駆動軸２は、下端が下部軸受４Ａを介して回転自在に培養槽１の底部に支持され、また上端が上部軸受４Ｂを介して回転自在に培養槽１の天井部に支持されている。   The culture tank 1 is a bottomed and cylindrical container, and is fixed so that the center axis is in the vertical direction as shown in the figure. A predetermined amount of the culture solution L is stored inside the culture tank 1. The culture solution L is a liquid that contains cells to be cultured (more precisely, a plurality of particulate cell pieces) and various nutrients that serve as nutrients for the cells. The drive shaft 2 is a rod-like member extending in the vertical direction along the central axis of the culture tank 1, and the stirring blades 3A and 3B are fixed at two locations in the middle. The lower end of the drive shaft 2 is rotatably supported by the bottom of the culture tank 1 via the lower bearing 4A, and the upper end of the drive shaft 2 is rotatably supported by the ceiling of the culture tank 1 via the upper bearing 4B. Yes.

２つの攪拌翼３Ａ、３Ｂは、上述したように駆動軸２の途中部位の２箇所、つまり上下方向に延在する駆動軸２に上下２段に固定されている。これら２つの攪拌翼３Ａ、３Ｂのうち、攪拌翼３Ａは下段に設けられており、攪拌翼３Ｂは上段に設けられている。これら２つの攪拌翼３Ａ、３Ｂは、図示するように、互いに直交する４つのパドルからなるパドル翼である。また、図１に示すように、下段に位置する攪拌翼３Ａと上段に位置する攪拌翼３Ｂとは、各々のパドルの駆動軸２に対する傾きが逆向きになっている。すなわち、下段に位置する攪拌翼３Ａの各パドルは駆動軸２に対して角度＋αの傾きを有し、上段に位置する攪拌翼３Ｂの各パドルは駆動軸２に対して角度−αの傾きを有している。   As described above, the two stirring blades 3A and 3B are fixed in two upper and lower stages on the drive shaft 2 extending in the vertical direction at two locations in the middle of the drive shaft 2. Of these two stirring blades 3A and 3B, the stirring blade 3A is provided in the lower stage, and the stirring blade 3B is provided in the upper stage. These two stirring blades 3A and 3B are paddle blades composed of four paddles orthogonal to each other as shown in the figure. Further, as shown in FIG. 1, the lower stirrer blade 3A and the upper stirrer blade 3B have the inclinations of the respective paddles with respect to the drive shaft 2 reversed. That is, each paddle of the stirring blade 3A located at the lower stage has an inclination of an angle + α with respect to the drive shaft 2, and each paddle of the stirring blade 3B located at the upper stage has an inclination of an angle −α with respect to the driving shaft 2. Have.

このような２つの攪拌翼３Ａ、３Ｂのうち、下段に位置する攪拌翼３Ａは、駆動軸２周りに回転することにより培養槽１の下部に培養液Ｌの対流（矢印で示す下部対流ＲＬ）を発生させ、また上段に位置する攪拌翼３Ｂは、同じく駆動軸２周りに回転することにより培養槽１の上部に培養液Ｌの対流（矢印で示す上部対流ＲＨ）を発生させる。 Of these two stirring blades 3A and 3B, the lower stirring blade 3A rotates around the drive shaft 2 to cause convection of the culture medium L in the lower part of the culture tank 1 (lower convection R L indicated by an arrow). The stirring blade 3B located in the upper stage also rotates around the drive shaft 2 to generate convection of the culture medium L (upper convection R H indicated by an arrow) in the upper part of the culture tank 1.

また、これら２つの攪拌翼３Ａ、３Ｂの位相差θは、図２に示すように、培養槽１の内側面近傍の各所（水平面内の各所）における下部対流ＲＬ及び上部対流ＲＨの衝突高さＨが各攪拌翼３Ａ、３Ｂの中間位置近傍となるように最適設定されている。各攪拌翼３Ａ、３Ｂは、何れも駆動軸２に固定されており、回転速度はお互いに同一なので、各攪拌翼３Ａ、３Ｂによって発生する下部対流ＲＬ及び上部対流ＲＨの流速は略同一と考えられる。 Further, as shown in FIG. 2, the phase difference θ between these two stirring blades 3A and 3B is a collision between the lower convection RL and the upper convection RH at various locations in the vicinity of the inner surface of the culture tank 1 (locations in the horizontal plane). The height H is optimally set so as to be in the vicinity of the intermediate position between the stirring blades 3A and 3B. Since each of the stirring blades 3A and 3B is fixed to the drive shaft 2 and has the same rotational speed, the flow rates of the lower convection RL and the upper convection RH generated by the stirring blades 3A and 3B are substantially the same. it is conceivable that.

したがって、培養槽１の内側面近傍における下部対流ＲＬ及び上部対流ＲＨの衝突高さＨは、下部対流ＲＬ及び上部対流ＲＨの各流路長と位相差θとによって決定される。この両者のうち、下部対流ＲＬ及び上部対流ＲＨの各流路長は、培養液Ｌの液面高さが一定で駆動軸２における各攪拌翼３Ａ、３Ｂの取り付け位置が一定の場合には略一定と考えることができるので、攪拌翼３Ａと攪拌翼３Ｂとの位相差θを調整することによって、下部対流ＲＬ及び上部対流ＲＨの衝突高さＨを培養槽１の内側面近傍の各所（水平面内の各所）において各攪拌翼３Ａ、３Ｂの上下方向における位置の中間位置近傍に設定することができる。 Accordingly, the height H collision of the lower convection R L and an upper convection R H in the inner surface near the culture tank 1 is determined by the lower convection R L and an upper convection R each flow path length of the H and the phase difference theta. Among these, the flow path lengths of the lower convection RL and the upper convection RH are such that the liquid level height of the culture medium L is constant and the mounting positions of the stirring blades 3A and 3B on the drive shaft 2 are constant. Can be considered to be substantially constant, the collision height H of the lower convection RL and the upper convection RH is adjusted in the vicinity of the inner surface of the culture tank 1 by adjusting the phase difference θ between the stirring blade 3A and the stirring blade 3B. In each of these locations (each location in the horizontal plane), it can be set in the vicinity of an intermediate position in the vertical direction of each stirring blade 3A, 3B.

下部軸受４Ａは、駆動軸２の下端を回転自在に培養槽１の底部に支持する。上部軸受４Ｂは、駆動軸２の上端を回転自在に培養槽１の天井部に支持する。これら下部軸受４Ａ及び上部軸受４Ｂのうち、下部軸受４Ａは、培養液Ｌに浸漬された状態で駆動軸２の下端部を回転自在に支持するもの、つまり培養液Ｌ中で軸受けとして機能するものなので、培養液Ｌ中に細胞の培養に悪影響を与えるような不要成分（潤滑油等）を放出しない構造のものが採用される。   The lower bearing 4A supports the lower end of the drive shaft 2 on the bottom of the culture tank 1 so as to be rotatable. The upper bearing 4 </ b> B supports the upper end of the drive shaft 2 on the ceiling of the culture tank 1 so as to be rotatable. Of these lower bearing 4A and upper bearing 4B, the lower bearing 4A is a member that rotatably supports the lower end of the drive shaft 2 while being immersed in the culture solution L, that is, a member that functions as a bearing in the culture solution L. Therefore, a structure that does not release unnecessary components (such as lubricating oil) that adversely affect cell culture in the culture solution L is employed.

連結器５は、駆動軸２の上端と駆動モータ６とを機械的に連結するものである。この連結器５は、例えば減速機能を有する減速器であり、駆動モータ６の回転を一定の減速比で駆動軸２に伝達する。駆動モータ６は、駆動軸２を回転駆動する動力源である。この駆動モータ６は、図示しない駆動回路から入力される駆動電流に基づいて駆動軸２を一定の回転速度で回転駆動する。   The coupler 5 mechanically connects the upper end of the drive shaft 2 and the drive motor 6. The coupler 5 is a speed reducer having a speed reducing function, for example, and transmits the rotation of the drive motor 6 to the drive shaft 2 at a constant speed reduction ratio. The drive motor 6 is a power source that rotationally drives the drive shaft 2. The drive motor 6 rotationally drives the drive shaft 2 at a constant rotational speed based on a drive current input from a drive circuit (not shown).

散気部７は、培養槽１の底部近傍に設けられ、酸素の気泡Ｇを培養槽１内（つまり培養液Ｌ中）に噴出する。この散気部７は、中空かつ多数の開口が表面に形成されたドーナツ状部材であり、外部の酸素供給源（図示略）から供給された酸素（Ｏ２）を各開口から培養液Ｌ中に噴射する。４つの邪魔板８Ａ〜８Ｄは、培養槽１の内側面に垂直姿勢で等間隔に設けられた板状部材である。 The air diffuser 7 is provided in the vicinity of the bottom of the culture tank 1 and jets oxygen bubbles G into the culture tank 1 (that is, in the culture medium L). The air diffuser 7 is a donut-shaped member that is hollow and has a large number of openings formed on the surface thereof. Oxygen (O 2 ) supplied from an external oxygen supply source (not shown) is passed through each opening into the culture medium L. To spray. The four baffle plates 8 </ b> A to 8 </ b> D are plate-like members provided at equal intervals in a vertical posture on the inner surface of the culture tank 1.

すなわち、これら邪魔板８Ａ〜８Ｄは、図２に示すように、駆動軸２を中心として互いに９０°の角度に相当する培養槽１の内側面の４箇所に、垂直方向に延在するように設けられている。伝熱ジャケット９は、培養槽１の外周を覆うように設けられており、培養槽１を介して内部の培養液Ｌの温度を所定温度に維持するためのものである。   That is, as shown in FIG. 2, these baffle plates 8 </ b> A to 8 </ b> D extend vertically at four locations on the inner surface of the culture tank 1 corresponding to an angle of 90 ° with respect to the drive shaft 2. Is provided. The heat transfer jacket 9 is provided so as to cover the outer periphery of the culture tank 1, and is for maintaining the temperature of the culture medium L inside the culture tank 1 at a predetermined temperature.

次に、このように構成された細胞培養装置Ａの作用効果について、図１を参照して詳しく説明する。   Next, the effect of the cell culture apparatus A configured as described above will be described in detail with reference to FIG.

本細胞培養装置Ａの運転状態では、酸素供給源から散気部７に酸素が供給され、これによって散気部７の各開口から培養液Ｌに酸素の気泡Ｇが噴射される。一方、このような培養液Ｌ中への気泡Ｇの噴射と並行して、駆動モータ６が作動することによって鉛直方向に離間する２つの攪拌翼３Ａ、３Ｂが培養液Ｌ中で回転運動を行う。   In the operating state of the cell culture apparatus A, oxygen is supplied from the oxygen supply source to the air diffuser 7, whereby oxygen bubbles G are jetted from the respective openings of the air diffuser 7 into the culture solution L. On the other hand, in parallel with the jetting of the bubbles G into the culture medium L, the two agitating blades 3A and 3B that are separated in the vertical direction by the operation of the drive motor 6 rotate in the culture medium L. .

そして、２つの攪拌翼３Ａ、３Ｂのうち、下段に位置する攪拌翼３Ａが回転運動を行うことによって、培養液Ｌは、図１に矢印で示すように、攪拌翼３Ａの近傍において上から下に向けて対流し、培養槽１の内側面近傍において下から上に向けて対流する。一方、上段に位置する攪拌翼３Ｂが回転運動を行うことによって、培養液Ｌは、図１に矢印で示すように、攪拌翼３Ｂの近傍において下から上に向けて対流し、培養槽１の内側面近傍において上から下に向けて対流する。また、このような培養液Ｌの各対流は、各攪拌翼３Ａ、３Ｂの回転によって発生するものなので、攪拌翼３Ａ、３Ｂの回転方向の速度成分を含むものである。   Then, among the two stirring blades 3A and 3B, the stirring blade 3A located at the lower stage performs a rotational movement, so that the culture solution L is located in the vicinity of the stirring blade 3A from the top as shown by the arrow in FIG. The convection is performed in the vicinity of the inner surface of the culture tank 1 from the bottom to the top. On the other hand, when the stirring blade 3B located in the upper stage performs a rotational motion, the culture solution L convects from the bottom to the top in the vicinity of the stirring blade 3B as shown by an arrow in FIG. Convection from top to bottom near the inner surface. In addition, each convection of the culture solution L is generated by the rotation of the stirring blades 3A and 3B, and therefore includes a velocity component in the rotation direction of the stirring blades 3A and 3B.

そして、下段に位置する攪拌翼３Ａの回転に起因する培養槽１の内側面近傍における下から上に向けた下部対流ＲＬと上段に位置する攪拌翼３Ｂの回転に起因する培養槽１の内側面近傍における上から下に向けた下部対流ＲＨとは、上下方向における２つの攪拌翼３Ａ、３Ｂの位置の中間位置近傍で衝突する。 The lower convection RL directed from the bottom to the top in the vicinity of the inner surface of the culture tank 1 caused by the rotation of the stirring blade 3A located in the lower stage and the inside of the culture tank 1 caused by the rotation of the stirring blade 3B located in the upper stage The lower convection RH from the top to the bottom near the side surface collides in the vicinity of the middle position between the two stirring blades 3A and 3B in the vertical direction.

そして、この衝突によって、下段に位置する攪拌翼３Ａの回転に起因する培養槽１の内側面近傍における下から上に向けた下部対流ＲＬは、培養槽１の内側面近傍から培養槽１の中心方向（駆動軸２の方向）に向きを変える。そして、この攪拌翼３Ａの回転に起因する下部対流ＲＬは、攪拌翼３Ａの上方に来ると、攪拌翼３Ａの回転に起因する吸引力によって攪拌翼３Ａの方向に向きを変え、攪拌翼３Ａに向けて流れ込む。 Due to this collision, the lower convection RL from the bottom to the top in the vicinity of the inner surface of the culture tank 1 caused by the rotation of the stirring blade 3A located in the lower stage is caused to flow from the vicinity of the inner surface of the culture tank 1 to the culture tank 1. The direction is changed in the center direction (direction of the drive shaft 2). Then, when the lower convection RL caused by the rotation of the stirring blade 3A comes above the stirring blade 3A, the direction is changed in the direction of the stirring blade 3A by the suction force caused by the rotation of the stirring blade 3A. Flow towards the.

一方、上段に位置する攪拌翼３Ｂの回転に起因する培養槽１の内側面近傍における上から下に向けた上部対流ＲＨは、培養槽１の内側面近傍から培養槽１の中心方向（駆動軸２の方向）に向きを変える。そして、この攪拌翼３Ｂの回転に起因する上部対流ＲＨは、攪拌翼３Ｂの下方に来ると、攪拌翼３Ｂの回転に起因する吸引力によって攪拌翼３Ｂの方向に向きを変え、攪拌翼３Ｂに向けて流れ込む。 On the other hand, the upper convection RH from the upper side to the lower side in the vicinity of the inner surface of the culture tank 1 due to the rotation of the stirring blade 3B located in the upper stage is from the vicinity of the inner surface of the culture tank 1 to the center direction (drive) Change the direction to the direction of axis 2). Then, when the upper convection RH resulting from the rotation of the stirring blade 3B comes below the stirring blade 3B, the direction is changed to the direction of the stirring blade 3B by the suction force resulting from the rotation of the stirring blade 3B, and the stirring blade 3B Flow towards the.

すなわち、培養槽１内においては、培養液Ｌに上下２つの対流（下部対流ＲＬ及び上部対流ＲＨ）が発生し、これら下部対流ＲＬ及び上部対流ＲＨは、互いに対流方向が逆向きである。下部対流ＲＬは、攪拌翼３Ａにおいて上から下に向かう流れであり、一方、上部対流ＲＨは、攪拌翼３Ｂにおいて下から上に向かう流れである。 That is, in the culture tank 1, two upper and lower convections (lower convection RL and upper convection RH ) are generated in the culture medium L, and the lower convection RL and the upper convection RH are opposite to each other. It is. The lower convection RL is a flow from top to bottom in the stirring blade 3A, while the upper convection RH is a flow from bottom to top in the stirring blade 3B.

このような下部対流ＲＬ及び上部対流ＲＨは、培養槽１の底部近傍の散気部７から噴射された多数の気泡Ｇに対して培養液Ｌの表面（上面）に容易に浮上させないように作用する。すなわち、散気部７から噴射された多数の気泡Ｇは、散気部７の上に位置する下段の攪拌翼３Ａに起因する下部対流ＲＬによって浮上に抗する力が作用し、容易に浮上することができない。 Such lower convection RL and upper convection RH do not easily float on the surface (upper surface) of the culture solution L with respect to a large number of bubbles G ejected from the air diffuser 7 near the bottom of the culture tank 1. Act on. That is, a large number of bubbles G ejected from the air diffuser 7 act as a force against the levitation due to the lower convection RL caused by the lower stirring blade 3A located above the air diffuser 7 and easily float. Can not do it.

また、気泡Ｇは、下部対流ＲＬによって培養槽１の内側面近傍に運ばれるが、上段の攪拌翼３Ｂに起因する上部対流ＲＨが下部対流ＲＬに上方から衝突するので、培養槽１の内側面近傍においてそのまま浮上することはできず、下部対流ＲＬによって培養槽１の中心方向に運ばれ、下段の攪拌翼３Ａの吸引力によって当該攪拌翼３Ａの下方に移動する。すなわち、散気部７から噴射された多数の気泡Ｇの殆どは、下部対流ＲＬに沿って培養槽１の下部で巡回運動を行い、容易に浮上しない。 The bubble G is carried by the lower convection RL to the vicinity of the inner surface of the culture tank 1, but the upper convection RH caused by the upper stirring blade 3B collides with the lower convection RL from above, so that the culture tank 1 It cannot float as it is in the vicinity of the inner surface, but is carried toward the center of the culture tank 1 by the lower convection RL , and moves below the stirring blade 3A by the suction force of the lower stirring blade 3A. That is, most of the many bubbles G ejected from the air diffuser 7 perform a circular motion in the lower part of the culture tank 1 along the lower convection RL and do not easily float.

また、下部対流ＲＬに沿って培養槽１の内側面近傍に運ばれた気泡Ｇの一部は、上部対流ＲＨとの衝突の際に当該対流ＲＨ内に取り込まれる。しかしながら、上部対流ＲＨ内に取り込まれた気泡Ｇは、当該上部対流ＲＨに沿って培養槽１の中心方向に運ばれるので、容易に浮上することができない。 Further, a part of the bubbles G carried along the lower convection RL to the vicinity of the inner surface of the culture tank 1 is taken into the convection RH when colliding with the upper convection RH . However, bubbles G taken into the upper convection R H is, since the conveyed toward the center of the culture tank 1 along the upper convection R H, not readily floated.

そして、上部対流ＲＨ内に取り込まれた気泡Ｇは、上段の攪拌翼３Ｂの吸引力によって当該攪拌翼３Ｂの上方に移動するが、上部対流ＲＨの勢いが原因となってそのまま浮上するものは少なく、殆どが上部対流ＲＨに沿って培養槽１の内側面近傍に移動して下降し、下部対流ＲＬと衝突することによって上部対流ＲＨに沿って培養槽１の上部で巡回運動を行い、容易に浮上しない。 The bubbles G taken into the upper convection RH move above the stirring blade 3B by the suction force of the upper stirring blade 3B, but rise as it is due to the momentum of the upper convection RH. Most of them are moved along the upper convection RH to the vicinity of the inner surface of the culture tank 1 and descend, and collide with the lower convection RL to make a circular motion in the upper part of the culture tank 1 along the upper convection RH. And does not surface easily.

さらに、下部対流ＲＬ及び上部対流ＲＨは、攪拌翼３Ａ、３Ｂの回転方向の速度成分を含むので、培養槽１の側部においては、各邪魔板８Ａ〜８Ｄが障害物として作用することにより流れが乱される。 Furthermore, since the lower convection RL and the upper convection RH include velocity components in the rotational direction of the stirring blades 3A and 3B, the baffle plates 8A to 8D act as obstacles on the side of the culture tank 1. Disturbs the flow.

このような細胞培養装置Ａによれば、培養槽１の底部近傍の散気部７から噴射された気泡Ｇは、その上方に上下２段に設けられた攪拌翼３Ａ、３Ｂに起因する培養液Ｌの下部対流ＲＬ及び上部対流ＲＨによって容易に浮上することができず、つまり培養液Ｌ中における滞留時間が従来よりも大幅に長くする。したがって、本細胞培養装置Ａによれば、培養液Ｌ中への酸素の溶け込み量を従来よりも大幅に増大させることができる。 According to such a cell culture apparatus A, the bubbles G ejected from the air diffuser 7 near the bottom of the culture tank 1 are the culture solution caused by the stirring blades 3A and 3B provided above and below in two stages. The lower convection RL and the upper convection RH cannot easily float, that is, the residence time in the culture medium L is significantly longer than before. Therefore, according to the present cell culture apparatus A, the amount of oxygen dissolved in the culture medium L can be significantly increased as compared with the prior art.

また、下部対流ＲＬ及び上部対流ＲＨが上下方向における各攪拌翼３Ａ、３Ｂの中間位置で衝突するように、攪拌翼３Ａと攪拌翼３Ｂとの位相差θが調整されているので、下部対流ＲＬに沿って移動する気泡Ｇが上部対流ＲＨに取り込まれる割合を小さくすることが可能であり、これによっても培養液Ｌ中における気泡Ｇの滞留時間を長くすることができる。 Further, since the phase difference θ between the stirring blade 3A and the stirring blade 3B is adjusted so that the lower convection RL and the upper convection RH collide with each other at an intermediate position between the stirring blades 3A and 3B in the vertical direction, It is possible to reduce the rate at which the bubbles G moving along the convection RL are taken into the upper convection RH , and this can also increase the residence time of the bubbles G in the culture solution L.

仮に、位相差θが最適設定されてなく、各攪拌翼３Ａ、３Ｂの中間位置から大幅にズレた位置で下部対流ＲＬと上部対流ＲＨとが衝突した場合、例えば上下方向における攪拌翼３Ａあるいは攪拌翼３Ｂの位置と同じような高さで下部対流ＲＬと上部対流ＲＨとが衝突した場合には、下部対流ＲＬに沿って移動する気泡Ｇのうち上部対流ＲＨに取り込まれる割合が多くなるので、気泡Ｇの滞留時間が比較的短い気泡Ｇの割合が増加する。 If the phase difference θ is not optimally set and the lower convection RL collides with the upper convection RH at a position significantly deviated from the intermediate position between the stirring blades 3A and 3B, for example, the stirring blade 3A in the vertical direction Alternatively, when the lower convection RL collides with the upper convection RH at the same height as the position of the stirring blade 3B, the bubbles G moving along the lower convection RL are taken into the upper convection RH. Since the ratio increases, the ratio of the bubbles G having a relatively short residence time increases.

また、下部対流ＲＬに沿って移動する気泡Ｇの一部は邪魔板８Ａ〜８Ｄの表面に沿って上昇するが、下部対流ＲＬに対向する上部対流ＲＨによって上昇が妨げられ、培養液Ｌの表面（上面）に容易に浮上することができない。 A part of the bubbles G moving along the lower convection RL rises along the surface of the baffle plates 8A to 8D, but the rise is hindered by the upper convection RH facing the lower convection RL , and the culture solution It cannot easily float on the surface (upper surface) of L.

なお、本発明は上記実施形態に限定されるものではなく、例えば以下のような変形例が考えられる。
（１）上記実施形態では、上下２段の攪拌翼３Ａ、３Ｂを備える構成を採用したが、本発明はこれに限定されない。攪拌翼の段数（個数）はさらに多くても良い。
（２）上記実施形態では、各攪拌翼Ａ、３Ｂとしてパドル翼を採用したが、本発明はこれに限定されない。細胞培養に供される攪拌翼には種々のものがあるので、それら各種の翼を攪拌翼として用いても良い。 In addition, this invention is not limited to the said embodiment, For example, the following modifications can be considered.
(1) In the embodiment described above, the configuration including the upper and lower two-stage stirring blades 3A and 3B is adopted, but the present invention is not limited to this. The number of stirring blades (number) may be larger.
(2) In the above embodiment, paddle blades are employed as the stirring blades A and 3B, but the present invention is not limited to this. Since there are various types of stirring blades used for cell culture, these various blades may be used as the stirring blades.

（３）上記実施形態では、２つの攪拌翼３Ａ、３Ｂの種類（形状）や回転数を全く同一としたが、本発明はこれに限定されない。
（４）上記実施形態では４つの邪魔板８Ａ〜８Ｄを設ける構成を採用したが、本発明はこれに限定されない。本発明では、多段に設けられた攪拌翼が邪魔板８Ａ〜８Ｄの機能をも奏するので、邪魔板８Ａ〜８Ｄを削除または個数を削減しても良い。 (3) In the above embodiment, the types (shapes) and rotation speeds of the two stirring blades 3A and 3B are exactly the same, but the present invention is not limited to this.
(4) In the above embodiment, the configuration in which the four baffle plates 8A to 8D are provided is adopted, but the present invention is not limited to this. In the present invention, the stirring blades provided in multiple stages also function as the baffle plates 8A to 8D. Therefore, the baffle plates 8A to 8D may be deleted or the number thereof may be reduced.

（５）上記実施形態では、攪拌翼３Ａと攪拌翼３Ｂとの位相差θを調整することにより、下部対流ＲＬ及び上部対流ＲＨの衝突高さＨを攪拌翼３Ａと攪拌翼３Ｂとの中間位置に設定したが、本発明はこれに限定されない。下部対流ＲＬの流路長と上部対流ＲＨの流路長との差を調整することによって攪拌翼３Ａと攪拌翼３Ｂとの中間位置で下部対流ＲＬ及び上部対流ＲＨが衝突するようにしても良い。 (5) In the above embodiment, the collision height H of the lower convection RL and the upper convection RH is adjusted between the stirring wing 3A and the stirring wing 3B by adjusting the phase difference θ between the stirring wing 3A and the stirring wing 3B. Although the intermediate position is set, the present invention is not limited to this. By adjusting the difference between the flow path length of the lower convection RL and the flow path length of the upper convection RH , the lower convection RL and the upper convection RH collide at an intermediate position between the stirring blade 3A and the stirring blade 3B. Anyway.

Ａ…細胞培養装置、１…培養槽、２…駆動軸、３Ａ、３Ｂ…攪拌翼、４Ａ…下部軸受、４Ｂ…上部軸受、５…連結器、６…駆動モータ、７…散気部、８Ａ〜８Ｄ…邪魔板、９…伝熱ジャケット、Ｌ…培養液、Ｇ…気泡（酸素）   DESCRIPTION OF SYMBOLS A ... Cell culture apparatus, 1 ... Culture tank, 2 ... Drive shaft, 3A, 3B ... Stirrer blade, 4A ... Lower bearing, 4B ... Upper bearing, 5 ... Coupler, 6 ... Drive motor, 7 ... Air diffuser, 8A ~ 8D ... baffle plate, 9 ... heat transfer jacket, L ... culture solution, G ... bubbles (oxygen)

Claims (5)

液体を貯留する攪拌槽と、
前記攪拌槽内に気泡を噴出する散気部と、
前記攪拌槽内に垂下された駆動軸と、
該駆動軸に多段に設けられた複数の攪拌翼と、を備え、
各段の攪拌翼は、互いに異なる方向の力を液体に与えることを特徴とする気液攪拌装置。 A stirring tank for storing liquid;
An air diffuser for ejecting bubbles into the stirring tank;
A drive shaft suspended in the stirring vessel;
A plurality of stirring blades provided in multiple stages on the drive shaft,
A gas-liquid stirring apparatus, wherein the stirring blades at each stage apply forces in different directions to the liquid. 前記攪拌翼は２段に設けられ、下段の攪拌翼は液体に下向きの力を与え、上段の攪拌翼は液体に上向きの力を与えることを特徴とする請求項１記載の気液攪拌装置。   2. The gas-liquid stirring apparatus according to claim 1, wherein the stirring blade is provided in two stages, the lower stirring blade applies a downward force to the liquid, and the upper stirring blade applies an upward force to the liquid. 前記各段の攪拌翼は、各方向に押し出された液体が上下方向における各段の攪拌翼の中間位置で衝突するように、所定の位相差θを持つ姿勢で前記駆動軸に取り付けられていることを特徴とする請求項１または２記載の気液攪拌装置。   The stirrer blades at each stage are attached to the drive shaft in a posture having a predetermined phase difference θ so that the liquid pushed out in each direction collides at an intermediate position between the stirrer blades at each stage in the vertical direction. The gas-liquid stirring apparatus according to claim 1 or 2, wherein 前記散気部は、前記攪拌槽の下部に設けられることを特徴とする請求項１〜３のいずれか一項に記載の気液攪拌装置。   The gas-liquid stirring device according to any one of claims 1 to 3, wherein the air diffuser is provided in a lower portion of the stirring tank. 前記攪拌槽の内壁において前記攪拌翼の間に位置する部位に設けられ、気泡の上昇を邪魔する邪魔板をさらに備えることを特徴とする請求項１〜４のいずれか一項に記載の気液攪拌装置。
The gas-liquid according to any one of claims 1 to 4, further comprising a baffle plate that is provided at a position located between the stirring blades on the inner wall of the stirring tank and prevents the bubbles from rising. Stirring device.
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