JP2010085151A - Light source device and photographic apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To attain size reduction and cost reduction in a light source device, while suppressing the occurrence of the speckle of a laser beam which is emitted as an exciting beam. <P>SOLUTION: An upper light source 14 is equipped with laser diodes 30a-30c, an exciting filter 31, diffusion members 32a-32c and a reflecting plate 33. The diffusion members 32a-32c, having a columnar shape have incident edge faces 35a crossing an axial direction at a right angle at their one ends and inclined edge faces 35b, inclined with respect to the incident edge faces 35a at their other ends. Each of the diffusion members 32a-32c is formed, by mixing a non-fluorescent granular member not emitting self-fluorescence with a low self-fluorescent transparent material, capable of neglecting the emission of self-fluorescence so as to disperse the same. The laser beams emitted from the laser diodes 30a-30c transmit through the exciting filter 31 to serve as exciting beams, and the exciting beams are incident on the incident-edge faces 35a of the diffusion members 32a-32c. The exciting beams incident on the inside from the incident-edge faces 35a are emitted from the inclined edge faces 35b or peripheral surfaces 35c in a diffused state. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a light source device and an imaging device that can image a fluorescent substance that is labeled on a living body.

  2. Description of the Related Art Conventionally, apparatuses that place a subject in a casing and shoot the subject by irradiating a living body with a light source provided in the casing have been used in various fields. In the fields of biochemistry and molecular biology, chemiluminescent substances and fluorescent substances are used as labeling substances, and by reading these luminescence and fluorescence, gene sequences, gene expression levels, protein separation, identification, or molecular weight There are known imaging apparatuses that perform characteristic evaluation and the like.

  In addition, a gene, protein, antibody, or pharmacological substance labeled with the above-described labeling substance is given to a living body such as a mouse or rat, and light from the labeling substance distributed in the body or body surface of the living body is photographed with a camera. 2. Description of the Related Art An imaging apparatus that performs so-called in vivo imaging for imaging gene expression, pharmacological action, and the like is known (for example, see Patent Document 1). In this photographing apparatus, for example, when labeling is performed with a fluorescent substance, the living body is irradiated with excitation light to excite the fluorescent substance, and the generated fluorescence is received by a camera to generate an image.

  When imaging a living body, the wavelength peak of the fluorescence generated by excitation appears longer than the wavelength peak of the excitation light irradiated to the living body, so the wavelength of the excitation light is blocked during observation and only the fluorescence spectral region is passed. Shoot using an optical filter.

  Conventional imaging apparatuses often use a halogen lamp or a light emitting diode (LED) as a light source of excitation light that irradiates a living body. For example, when a halogen lamp or a white LED is used, a bandpass filter When using a monochromatic LED with a narrow spectral width, the long wavelength component is cut with a dedicated optical filter. Excitation light irradiated through these optical filters may partially overlap with the wavelength of fluorescence generated by excitation. Since the excitation light having the same wavelength as the fluorescence enters the camera and is received, it becomes background noise of the image and cancels the weak fluorescent component. Therefore, it is difficult to perform high-precision observation that reads even weak fluorescence. Such a problem can be solved by using a strong light source having a narrow spectral wavelength width. However, it is considered to use a laser light source as an excitation light source that meets this condition.

  However, when a laser light source is used, unnatural illumination unevenness may appear in the image due to speckles generated during laser light illumination. Therefore, it is necessary to suppress the generation of speckles for use as an excitation light source. Become. Therefore, as a countermeasure against this speckle, in Patent Document 2, a resonator is disposed on the optical path of the laser beam, and the first partial light that is reflected when the laser beam hits the surface of the resonator and the laser beam enters the resonator. The laser beam is divided into second partial light that is emitted from the region of the incident position after being subjected to total reflection a plurality of times to the outside of the resonator, and the second partial light is further modulated in the resonator, thereby the resonator. A system for reducing the coherence of laser light emitted from a laser is described. Thereby, generation | occurrence | production of a speckle can be suppressed by reducing a laser beam coherence with a resonator. Patent Document 3 describes a configuration in which a light diffusing plate is installed on the optical path of the laser light and the laser light is diffused by vibrating and rotating the light diffusing plate to suppress speckle generation. .

JP 2003-287497 A Special table 2007-528595 gazette JP 2007-233371 A

  However, when the imaging apparatus is equipped with the configuration of the laser light source described in Patent Documents 2 and 3, the cost of the resonator and the mechanical mechanism that vibrates and rotates the light diffusing plate is high, and a space for incorporating these is necessary. Therefore, this increases the overall cost of the apparatus and hinders downsizing of the apparatus. Moreover, in the imaging system described in Patent Document 1, it is not considered to use a laser light source as an excitation light source, and naturally no speckle countermeasure is taken into consideration when a laser light source is used.

  The present invention has been made in view of the above problems, and a light source device and an imaging device capable of reducing the size and cost of the apparatus while suppressing speckle generation of laser light irradiated as excitation light. The purpose is to provide.

  In order to achieve the above object, a light source device of the present invention is a light source device that irradiates a living body with excitation light to excite a fluorescent substance and generates fluorescence that can be photographed with a camera, and a laser light source that emits excitation light And a self-fluorescent transparent material that can ignore the generation of self-fluorescence, and a non-fluorescent granular member that does not generate auto-fluorescence is mixed to form an excitation that is incident on the inside from the laser light source. And a diffusion member that diffuses light and irradiates the living body. The camera in the present invention may be any camera that generates an image from received fluorescence, such as a camera equipped with an image sensor such as a solid-state image sensor or a photographic camera that forms an image on a photographic film.

  The diffusing member has a substantially cylindrical shape, an incident end surface orthogonal to the axial direction is formed at one end, and an inclined end surface inclined with respect to the incident end surface is formed at the other end, and the peripheral surface and the inclined end surface are formed on the living body. It is preferable that the excitation light is arranged so as to face and enter the inside from the incident end face, and the living body is irradiated with the excitation light diffused from the peripheral surface and the inclined end face. Preferably, a reflecting plate is provided at a position opposite to the living body with respect to the diffusing member, and the excitation light emitted from the diffusing member is reflected by the reflecting plate to irradiate the living body. In addition, it is preferable that a plurality of the laser light sources are arranged along a direction orthogonal to the axial direction, and the diffusing members are respectively arranged at positions where excitation light from the laser light sources is incident.

  A reflection member configured to reflect the excitation light from the laser light source to the living body without passing through the diffusion member; a plurality of the laser light sources are provided, and excitation light from at least one laser diode is reflected by the reflection member by the reflection member; Is preferably irradiated.

  A reflection member that reflects excitation light from the laser light source to the living body without passing through the diffusion member, and the laser so that excitation light from one laser diode is incident on either the diffusion member or the reflection member. It is preferable to include a switching mechanism that switches the position of the light source.

  The granular member is preferably titanium oxide or barium sulfate. Alternatively, the granular member is preferably a transparent bead that reflects light at an interface with the transparent material. The transparent material is preferably silicon or quartz glass.

  An imaging device of the present invention includes the light source device according to any one of claims 1 to 9 and a camera that generates an image by receiving fluorescence of the living body generated by the excitation light.

  According to the present invention, a diffusion member formed by mixing a low autofluorescent transparent material so as to disperse a non-fluorescent granular member, and the excitation light incident on the inside of the diffusion member from the laser diode. Since it diffuses and irradiates a living body, it is possible to reduce the size and cost of the apparatus while suppressing speckles of laser light irradiated to the living body as excitation light.

  FIG. 1 shows the appearance of a photographing system embodying the present invention. The imaging system 2 receives light from a labeling substance distributed on the body surface of a living body 3 (see FIG. 2) such as a mouse or a rat (see FIG. 2) or a labeling substance distributed in the body (hereinafter referred to as an image of the labeling substance distributed on the living body 3). (Distributed image) is taken (generated) and displayed. In this embodiment, a case where a fluorescent substance that emits fluorescence by irradiating excitation light as a labeling substance is administered to the living body 3 and a distribution image of the fluorescent substance distributed in the living body 3 is generated will be described. The same applies to the case of generating a distribution image of a tissue that emits, for example, a tumor tissue.

  The photographing system 2 generates a distribution image, performs various settings on the photographing device 5 and performs various image processing on the distribution image generated by the photographing device 5 and displays the distribution image on the monitor 6a. It is composed of a PC (Personal Computer) 6 that performs recording on a medium. The PC 6 realizes a setting function, an image processing function, and the like for the photographing apparatus 5 by installing predetermined software.

  In FIG. 2, the photographing device 5 includes a housing 11, a camera unit 12, a photographing stage 13, an upper light source 14 (light source device), and a bottom light source 15. The casing 11 has a hollow, substantially rectangular parallelepiped shape, and is provided with a lid 16 (see FIG. 1) attached to the front face so as to be freely opened and closed. When the lid 16 is opened, the imaging room 17 provided inside the housing 11 is exposed so that the living body 3 can be accommodated, and the living body 3 can be taken out from the imaging room 17. When the lid 16 is closed, the inside of the photographing room 17 is shielded, and light other than fluorescence emitted from the living body 3 is prevented from entering the camera unit 12 during photographing.

  The imaging room 17 is provided with a camera unit 12 at the top. The camera unit 12 generates a distribution image of the living body 3 set on the imaging stage 13. The camera unit 12 includes an image sensor 18, a photographing lens 19, a camera side filter 20, a cooling unit 21, and the like. The image sensor 18 is the same as that used in a digital camera or the like, and a large number of light receiving elements are arranged in a matrix on the light receiving surface thereof. Specifically, a solid-state imaging device such as a CCD is used as the image sensor 18.

  In the case of generating a distribution image, as will be described later, the image sensor 18 is maintained in a state where photoelectric conversion is performed by a light receiving element and charges are accumulated, while fluorescence is received from a fluorescent substance, and the light is used as charges. Exposure is performed by accumulating.

  The taking lens 19 images the fluorescence from the living body 3 on the image sensor 18. The camera-side filter 20 is a filter that blocks excitation light from the upper light source 14 and the bottom light source 15 and transmits fluorescence from the fluorescent material distributed in the living body 3. As the camera-side filter 20, for example, a long-pass filter that cuts light having a shorter wavelength than the fluorescence from the fluorescent material is used, and only the fluorescence from the living body 3 is received by the image sensor 18. The cooling unit 21 reduces the dark current by cooling the image sensor 18 and suppresses noise in the generated distribution image.

  As the image sensor 18, various types such as a CCD type and a CMOS type can be used. Further, an image sensor using an organic photoelectric conversion film may be used in addition to a semiconductor that performs photoelectric conversion. Further, not only a single color but also a received light may be separated into a plurality of colors and photographed. The image sensor 18 is driven by the driver 23 under the control of the timing control unit 22.

  A shooting stage 13 is arranged directly below the camera unit 12. The photographing stage 13 has a box shape including a stage surface 24 on which the living body 3 is placed and a housing 25 below the stage surface 24. A bottom light source 15 is disposed in the hollow interior of the photographing stage 13. The stage surface 24 has a transparent plate shape so that the living body 3 placed thereon is irradiated with the excitation light from the bottom light source 15. The housing 25 is opaque so as to block the excitation light from the bottom light source 15 so that the excitation light is not emitted from other than the stage surface 24. As the bottom light source 15, various types such as a halogen lamp, a strobe discharge tube, a laser, and an LED can be used. In this example, the configuration includes a white LED and a short-pass filter that cuts light in the wavelength range of fluorescence from the fluorescent material and light on the longer wavelength side.

  An upper light source 14 is disposed obliquely above the photographing stage 13 and at a position symmetrical with respect to the photographing center of the camera unit 12. The upper light source 14 and the bottom light source 15 are excitation light sources that irradiate the living body 3 with excitation light for exciting and emitting the fluorescent material distributed in the living body 3. The upper light source 14 and the bottom light source 15 are driven by the corresponding upper light source driver 28 and bottom light source driver 29, respectively, under the control of the timing control unit 22.

  FIG. 3 shows the configuration of the upper light source 14 which is the light source device of the present invention. In the present embodiment, the upper light source 14 includes three laser diodes 30 a to 30 c, an excitation filter 31, three diffusion members 32 a to 32 c, and a reflection plate 33. The laser diodes 30a to 30c are turned on / off in accordance with the timing of photographing the living body under the control of the timing control unit 22. The reflecting plate 33 is fixed to a stay 34 (see FIG. 2) provided in the housing 3, and the laser diodes 30a to 30c, the excitation filter 31, and the diffusion member 32 are also held by a holding member (not shown). And is fixed to the stay 34. Red laser diodes are used as the laser diodes 30a to 30c.

  The excitation filter 31 has a plate shape whose longitudinal direction is arranged along the arrangement direction of the laser diodes 30a to 30c. The excitation filter 31 cuts light in the long wavelength region from the laser light emitted from the laser diodes 30a to 30c. Only the light in the wavelength band is transmitted and used as excitation light. This prevents the wavelength of excitation light and the wavelength of fluorescence generated by excitation from overlapping. The laser diodes 30a to 30c are arranged along a direction orthogonal to the axial direction of the diffusion members 32a to 32c.

  Each of the diffusing members 32a to 32c has a columnar shape, an incident end surface 35a orthogonal to the axial direction is formed at one end, and an inclined end surface 35b that is inclined with respect to the incident end surface 35a is formed at the other end. The inclined end surface 35b and the peripheral surface 35c face the living body 3, and are arranged at positions where excitation light from the laser diodes 30a to 30c is incident from the incident end surface 35a toward the inside. .

  These diffusing members 32a to 32c are formed by mixing non-fluorescent granular members that do not generate autofluorescence in a low autofluorescent transparent material that can ignore the generation of autofluorescence. In this embodiment, silicon is used as the low autofluorescent transparent material forming the diffusion member, and titanium oxide, which is a white powder, is used as the non-fluorescent granular member. The transparent material for forming the diffusing member is not limited to silicon, and any transparent material having low autofluorescence can be used. For example, quartz glass can be used. When the diffusing members 32a to 32c are formed from such a material, the above-mentioned diffusing members 32a to 32c are mixed by mixing the molten transparent material so as to disperse the granular member, and then pouring into a mold and curing. The shape is formed. By using such diffusing members 32a to 32c, the excitation light incident from the incident end surface 35a passes through the transparent material and is reflected and diffused by the granular member, and is emitted from the inclined end surface 35b or the peripheral surface 35c. To do.

  The reflector 33 is located on the opposite side of the living body 3 with respect to the diffusing members 32a to 32c. The reflecting plate 33 is a plate formed in a substantially U-shaped cross section composed of a bottom surface portion 33a positioned in parallel with the diffusing members 32a to 32c and side surface portions 33b and 33c on both sides, and from the diffusing members 32a to 32c. The emitted excitation light is reflected and irradiated on the living body 3.

  The upper light source driver 28 and the bottom light source driver 29 turn on the light source while the pulse timing signal is input from the timing control unit 22. The living body 3 is irradiated with excitation light from an excitation light source to generate fluorescence from the fluorescent material. The living body may be irradiated with excitation light based on the timing signal by opening and closing the shutter or the like while the light source is turned on (lit state).

  Next, the operation of the above configuration will be described. First, the user opens the lid 16, and the living body 3 to which the fluorescent substance has been administered is placed on the imaging stage 13. After the user closes the lid 16 and the photographing room 17 is in a light-shielded state, the user operates the PC 6 to select and set which of the upper light source 14 and the bottom light source 15 is used, and instructs to start photographing. Hereinafter, a case where the upper light source 14 according to the present invention is selected will be described in detail. When an instruction to start photographing is given, the timing control unit 22 sends an exposure start signal to the driver 23.

  When the driver 23 receives the exposure start signal, the image sensor 18 starts to operate, and the light received by each light receiving element is photoelectrically converted into electric charge and accumulated. On the other hand, the timing control unit 22 starts processing for generating a timing signal. Each timing signal generated in this process is sequentially sent to the driver of the selected light source. For example, when the selected light source is the upper light source 14, each timing signal is sequentially sent to the upper light source driver 28.

  When the timing signal is input to the upper light source driver 28, the upper light source 14 is turned on in response to the input of the timing signal, and excitation light is irradiated toward the living body 3. Then, the fluorescence emitted from the fluorescent material by the irradiation is received by the image sensor 18 through the camera-side filter 20 and the photographic lens 19, and the charge due to the received light is gradually accumulated. The charge accumulated in the image sensor 18 is photoelectrically converted to generate an image, which is sent to the PC 6.

  As described above, the laser light emitted from the laser diodes 30a to 30c is irradiated as excitation light, and the excitation light is irradiated to the living body 3 in a state of being diffused by the diffusion members 32a to 32c. It is possible to prevent the excitation light from overlapping the wavelength and to prevent speckle from occurring. As a result, the image generated from the charges accumulated in the image sensor 18 can read even weak fluorescence, and illumination unevenness due to speckle is eliminated, so that the living body 3 can be observed with a high-quality image. Can do. Furthermore, the upper light source 14 can diffuse and irradiate the excitation light with a simple configuration without using a complicated configuration such as a resonator or a mechanical mechanism, thereby preventing speckles from being generated. Miniaturization and low cost can be achieved.

  In the above embodiment, only the single-color red laser diodes are used as the laser diodes 30a to 30c. However, the present invention is not limited to this, and a laser diode corresponding to each color of red, green, and blue may be used. .

  In the above embodiment, the upper light source 14 (light source device) includes the diffusing members 32a to 32c respectively corresponding to the laser diodes 30a to 30c. However, the present invention is not limited to this, and as shown in FIG. It is good also as a structure provided with the one diffusion member 36 into which the excitation light irradiated from 30a-30c injects. In this case, the diffusing member 36 has a wide rectangular parallelepiped shape that matches the arrangement of the laser diodes 30a to 30c, and has an incident end surface 37a orthogonal to the axial direction at one end and an inclined end surface that is inclined with respect to the incident end surface 37a at the other end. 37 b is formed, and the inclined end surface 37 b and the upper surface 37 c are arranged so as to face the living body 3. Similar to the diffusion members 32a to 32c of the above-described embodiment, the diffusion member 36 is formed by mixing a low autofluorescent transparent material so as to disperse a non-fluorescent granular member, and the laser diodes 30a to 30a. The excitation light from 30c enters the inside of the diffusing member 36 from the incident end surface 37a, is reflected by the granular member, and is irradiated to the living body from the inclined end surface 37b and the upper surface 37c in a diffused state.

  In the above embodiment, a plurality of laser diodes are arranged in the upper light source 14 (light source device), and the diffusing members are arranged at positions where excitation light from the laser diode is incident, but the present invention is not limited to this. A configuration may be adopted in which excitation light from at least one laser diode among a plurality of laser diodes is irradiated on a living body without passing through a diffusion member. An upper light source to which this configuration is applied is shown in FIG. In FIG. 5, the same components and members as those in the above embodiment are denoted by the same reference numerals and description thereof is omitted. In this case, the upper light source 40 (light source device) includes laser diodes 41a to 41c, a reflecting mirror 42 arranged in parallel with the two diffusion members 32a and 32b, and a collimator lens 43. Further, as the laser diodes 41a to 41c, for example, IR (infrared) laser diodes are used. Excitation light emitted from the two laser diodes 41a and 41b enters the diffusing members 32a and 32b, respectively. The excitation light emitted from the one laser diode 41c is collimated by the collimator lens 43 and reflected by the reflecting mirror. 42 is incident. The reflecting mirror 42 is a reflecting member that can reflect the excitation light, and is arranged so that the optical path of the excitation light is bent by 90 ° and irradiated to the living body 3. With such a configuration, the excitation light emitted from the laser diode 41c is emitted to the living body 3 without going through the diffusing member, so that it can be used as a spotlight with a higher light quantity. In normal times, the laser diodes 41a and 41b are turned on to irradiate the living body 3 with excitation light via the diffusing members 32a and 32b. When a higher light quantity is required, the laser diode 41c is turned on by an instruction from the PC 6. Switching control is performed so that the living body 3 is directly irradiated with excitation light.

  In the above-described embodiment, a plurality of laser diodes are provided, and a diffusing member or a reflecting member corresponding to each laser diode is provided. However, the present invention is not limited to this, and excitation light from one laser diode is emitted. It is good also as a structure switched so that it may inject into either a diffusion member or a reflection member. An upper light source to which this configuration is applied is shown in FIG. In this case, the upper light source 50 (light source device) includes a diffusing member 32a, a reflecting mirror 42, a single laser diode 51, an excitation filter 52, a housing 53 that holds the laser diode 51 and the excitation filter 52, and a housing. And a switching mechanism 54 for switching the position of 53. The switching mechanism 54 includes, for example, a drive motor, a belt, a pulley, and the like, and is between an indirect irradiation position where excitation light irradiated from the laser diode 51 enters the diffusing member 32a and a direct irradiation position where the reflection light enters the reflecting mirror 42. The position of the housing 53 is switched. In normal times, the housing 53 is moved to the indirect irradiation position to irradiate the living body 3 with the excitation light from the laser diode 51 via the diffusing member 32a. When a stronger light quantity is required, an instruction from the PC 6 is used. Switching control of the switching mechanism 54 is performed so that the housing 53 is directly switched to the irradiation position and the living body 3 is directly irradiated with the excitation light.

  In addition, in the said embodiment, although the structure which applied this invention to the upper light source is enumerated, you may apply not only to this but to a bottom light source. Moreover, although the structure which uses a laser diode as a laser light source is enumerated, this invention is not restricted to this, It can apply also to the structure which consists of other laser light sources, such as solid lasers and gas lasers other than a laser diode.

  Furthermore, the granular member is not limited to titanium oxide, and may be any non-fluorescent white powder. For example, barium sulfate may be used. Alternatively, instead of these white powders, transparent beads (diffusion bodies) described in JP-A-2003-107217 or JP-A-6-347616 may be used as the granular member. Since these transparent beads reflect and diffuse light at the boundary surface with the transparent material, it is possible to obtain the same effect as in the above embodiment.

  In the above embodiment, the configuration of a camera provided with an image sensor is exemplified as a camera used in the photographing apparatus. However, the present invention is not limited to this, and a photograph that generates an image on a photographic film from received fluorescence. A camera may be used.

It is a perspective view which shows the structure of the imaging | photography system which implemented this invention. It is a block diagram which shows the outline of a structure of an imaging device. It is a perspective view which shows the structure of a light source device. It is a perspective view which shows the structure of the modification of 1st Embodiment. It is a perspective view which shows the structure of the light source of 2nd Embodiment. It is a perspective view which shows the structure of the light source of 3rd Embodiment.

Explanation of symbols

2 Imaging System 3 Living Body 5 Imaging Device 12 Camera Unit 14, 40, 50 Upper Light Source (Light Source Device)
15 Bottom light source 30a-30c, 41, 51 Laser diode (laser light source)
32a-32c Diffusing member 35a, 37a Incident end face 35b, 37b Inclined end face 42 Reflecting mirror (reflecting member)

Claims (10)

A light source device that irradiates a living body with excitation light to excite a fluorescent material and generates fluorescence that can be photographed with a camera,
Formed by mixing a laser light source that emits excitation light and a low autofluorescent transparent material that can ignore the generation of autofluorescence so as to disperse non-fluorescent granular members that do not generate autofluorescence. A light source device comprising: a diffusion member that diffuses excitation light incident on the inside and irradiates the living body.   The diffusing member has a substantially cylindrical shape, an incident end surface orthogonal to the axial direction is formed at one end, and an inclined end surface inclined with respect to the incident end surface is formed at the other end, and the peripheral surface and the inclined end surface are formed on the living body. The pumping light is disposed so as to face and enter from the incident end surface toward the inside, and the living body is irradiated with the excitation light diffused from a peripheral surface and the inclined end surface. The light source device described.   2. The reflection plate is provided at a position opposite to the living body with respect to the diffusion member, and the excitation light emitted from the diffusion member is reflected by the reflection plate to irradiate the living body. Or the light source device of 2.   The plurality of laser light sources are arranged along a direction orthogonal to the axial direction, and the diffusing members are respectively arranged at positions where excitation light from the laser light sources is incident. The light source device according to any one of 1 to 3.   A reflection member configured to reflect the excitation light from the laser light source to the living body without passing through the diffusion member; a plurality of the laser light sources are provided, and excitation light from at least one laser diode is reflected by the reflection member by the reflection member; The light source device according to claim 1, wherein the light source device is irradiated with a light source.   A reflection member that reflects excitation light from the laser light source to the living body without passing through the diffusion member, and the laser so that excitation light from one laser diode is incident on either the diffusion member or the reflection member. The light source device according to claim 1, further comprising a switching mechanism that switches a position of the light source.   The light source device according to claim 1, wherein the granular member is titanium oxide or barium sulfate.   The light source device according to claim 1, wherein the granular member is a transparent bead that reflects light at an interface with the transparent material.   The light source device according to claim 1, wherein the transparent material is silicon or quartz glass.   An imaging apparatus comprising: the light source device according to claim 1; and a camera that generates an image by receiving fluorescence of the living body generated by the excitation light.

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